Daisuke Yasuda's research while affiliated with Showa University and other places

... Moreover, after the implantation, released 5-FU plays relevant functions after diffusing into extracellular tissue fluid; the extremely small amount of the drug entering into the blood through the capillary wall results in low peak concentration, which avoids toxic and side effects. [10][11][12][13] We found that sustained-release 5-FU could inhibit liver cancer and rapid proliferation of residual cancer cells after surgical resection, and sustained-release 5-FU conforming to the concept and requirement of regional chemotherapy and interstitial chemotherapy was operable and valuable. ...

... In this retrospective study, it was found that %FLVpost/pre-op was on average 83% and 91% among patients treated with and without pre-operative chemotherapy, respectively. The latter number (91%) may seem high, but several previous studies 45, 46 have reported similar numbers. Lower numbers are being reported as well, e.g. ...

... Initially introduced by Mirizzi in 1937, intraoperative cholangiography (IOC) is recommended during cholecystectomy to prevent biliary tract injuries [6]. In the field of human medicine, intraoperative near-infrared (NIR) fluorescent cholangiography using a real-time imaging technique with intravenous administration of indocyanine green (ICG) has been widely used to assess fluorescent images of the biliary tract, enabling real-time assessment of fluorescent images of the biliary tract, and reducing biliary tract injury [7]. ...

... to perform regional lymph node dissection using the stained area as a guide has been introduced for the treatment of gastric cancer [6,7]. Several studies have validated the safety and efficacy of this method [8,9]. ...

... Several sites, including the spleen, omentum, subcutaneous spaces, intraperitoneal space, and kidney subcapsular space, have been utilized for implantation. 6,7 However, the optimum site for encapsulated stem cell implantation is unclear. 8,9 In a landmark study, Lim and Sun successfully achieved normoglycemia by micro-encapsulating islet cells in a diabetic rat model. ...

... With multiple GCSF injections spaced out like what was shown in the Verma et al study, CD34+ cell mobilization and its singular role in amelioration of liver related events is difficult to explain. (23) Hence, without proper elucidation of GCSF mediated significant pathways that promote or ameliorate liver damage, studying only CD34+ cells in DC does not improve our understanding of the disease and its mechanisms and cannot be extrapolated to outcomes in such patients.In animal studies on GCSF, an improvement in serum albumin has been shown, with doubtful long-term effect while BMSC engraftment within the liver appears to be a temporary phenomenon.(24) In our patients, similar findings as that described in preclinical studies were reproduced. ...

... For instance, Nyberg et al. 12 compared albumin production per cell, and the albumin production of cryopreserved cells was 2% of that of fresh cells. The results of albumin synthesis analysis in this study are consistent with those of other past researchers [13][14][15] . ...

... Thus, alginate encapsulation provides an approach that combines the advantages of a stirred system with the prevalence of the microenviroment, which is key for differentiation. Moreover, alginate-based hydrogels have been previously applied for toxicological applications and tested with several drug compounds, without drug binding to the scaffold (Lan and starly 2011; Koizumi et al. 2007), making it a suitable system for pre-clinical development applications. ...

... However, no groups have thus far shown functional advantages on hepatocyte grafts. Unlike the previous reports (Hayashi et al., 2007), in the present study, we did not use commercially available Nagase analbuminemic rats (not syngeneic combination), instead we used mutant F344 rats with defective albumin production (kindly provided by Professor Nishikawa) (Fukuoka et al., 2017;Matsumura et al., 2019). Thus, we could focus on physiological engraftment without paying any attention to the immunological influences, since these mutant rats are a syngeneic combination. ...

... For instance, Nyberg et al. 12 compared albumin production per cell, and the albumin production of cryopreserved cells was 2% of that of fresh cells. The results of albumin synthesis analysis in this study are consistent with those of other past researchers [13][14][15] . ...