Cynthia Ghobril's scientific contributions
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Publications (2)
The β-carbonic anhydrase (CA, EC 4.2.1.1) from the genome of the opportunistic pathogen Malassezia restricta (MreCA), which was recently cloned and characterised, herein has been investigated for enzymatic activation by a panel of amines and amino acids. Of the 24 compounds tested in this study, the most effective MreCA activators were L-adrenaline...
The cloning, purification, and initial characterization of the β-carbonic anhydrase (CA, EC 4.2.1.1) from the genome of the opportunistic pathogen Malassezia restricta (MreCA), which a fungus involved in dandruff and seborrheic dermatitis (SD), is reported. MreCA is a protein consisting of 230 amino acid residues and shows high catalytic activity f...
Citations
... Thus, we measured the catalytic activity of EcoCAb at a pH of 8.3 and determined its kinetic constants (k cat and K M ) for comparison to those of the thoroughly studied human (h) CA isoforms hCA I and II, belonging to the a-CA class (Table 1) as well as a recently investigated pathogenic, fungal b-CA, from Malassezia restrica, MreCA 44 . The experiments were also performed in the presence of 10 mM L-Trp as an activator (Table 1) or in the presence of a sulphonamide inhibitor (data not shown). ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/531549712273408-1503742735715_Q64/Claudiu-Supuran.jpg)
... to ensure the integrity of the gene and absence of errors at the ligation sites, the vector containing the fragment underwent bidirectional automated sequencing. the pet100D-topo/tg_ ca vector was employed to transform competent escherichia coli Bl21 (De3) codon plus cells (agilent) 50 . the induced cellular culture with iPtG resulted in the overexpression of the recombinant tg_ca. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/272432439230474-1441964363210_Q64/Daniela-Vullo.jpg)
