Cong Shen's research while affiliated with State Key Laboratory of Medical Genetics of China and other places
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Publications (74)
Intervertebral disc degeneration (IDD) is a significant cause of low back pain, characterized by excessive senescence and apoptosis of nucleus pulposus cells (NPCs). However, the precise mechanisms behind this senescence and apoptosis remains unclear. This study aimed to investigate the role of Tbxt in IDD both in vitro and in vivo, using a hydroge...
Background
Visceral obesity elevates high-grade serous carcinoma (HGSOC) risk and prognosis but its effect on biology is underexplored. This study examines obesity-related genes (VORGs) in HGSOC.
Methods
Using consensus clustering, we analyzed TCGA and GSE53963 datasets with VORGs, uncovering unique immune cell patterns and gene functions. Our pro...
Metabolic dysfunction of the extracellular matrix (ECM) is one of the primary causes of intervertebral disc degeneration (IVDD). Previous studies have demonstrated that transcription factor Brachyury (Bry) has the potential to promote the synthesis of the collagen II and aggrecan, while the specific mechanism is still kept to be unknown. In this st...
RING finger 187 (RNF187), a ubiquitin-ligating (E3) enzyme, plays a crucial role in the proliferation of cancer cells. However, it remains unclear whether RNF187 exhibits comparable functionality in the development of germline cells. To investigate the potential involvement of RNF187 in germ cell development, we conducted interference and overexpre...
Thousands of genes are expressed in the testis of mice. However, the details about their roles during spermatogenesis have not been well-clarified for most genes. The purpose of this study was to examine the effect of Slc26a1 deficiency on mouse spermatogenesis and male fertility. Slc26a1 -knockout (KO) mice were generated using CRISPR/Cas9 technol...
As a common malignant tumor among women, ovarian cancer poses a serious threat to their health. This study demonstrates that long non-coding RNA NRSN2-AS1 is over-expressed in ovarian cancer tissues using patient sample and tissue microarrays. In addition, NRSN2-AS1 is shown to promote ovarian cancer cell proliferation and metastasis both in vitro...
Intervertebral disc degeneration (IDD) is a significant cause of low back pain, characterized by excessive senescence and apoptosis of nucleus pulposus cells (NPCs). However, the precise mechanisms behind this senescence and apoptosis remains unclear. This study aimed to investigate the role of Tbxt in IDD both in vitro and in vivo , using a hydrog...
Ubiquitination is the most common post‐translational modification and is essential for various cellular regulatory processes. RNF187, which is known as RING domain AP1 coactivator‐1, is a member of the RING finger family. RNF187 can promote the proliferation and migration of various tumor cells. However, whether it has a similar role in regulating...
Nucleus pulposus (NP) degeneration is characterized by the decreased cellularity of nucleus pulposus cells (NPCs) and diminished content of hydrophilic extracellular matrix (ECM). Overexpression of brachyury has been reported to reverse the degenerated NPCs into healthy phenotypes. However, the direct correlation between brachyury and ECM has not b...
As a common malignant tumor among women, ovarian cancer poses a serious threat to their health. This study demonstrates that long non-coding RNA NRSN2-AS1 is over-expressed in ovarian cancer tissues using patient sample and tissue microarrays. In addition, NRSN2-AS1 is shown to promote ovarian cancer cell proliferation and metastasis both in vitro...
Background:
Studies have shown that ferroptosis- and oxidative stress-related genes (FORGs) perform crosstalk in ovarian cancer (OC). The specific role of FORGs in OC, however, remains unclear. We aimed to develop a molecular subtype and prognostic model associated with FORGs that could predict OC prognosis and evaluate the infiltration of tumor-a...
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Despite the rapidly growing interest in nanoparticle-mediated controllable male contraception and recovery of male fertility, novel applications of nanoparticles in these processes are limited by a knowledge gap regarding their transport and distribution in the testes. Here, we investigated the fate of gold nanoparticles in the mouse testes using t...
During spermatogenesis, mitochondria extend along the whole length of spermatid tail and offer a structural platform for microtubule reorganization and synchronized spermatid individualization, that eventually helps to generate mature sperm in Drosophila. However, the regulatory mechanism of spermatid mitochondria during elongation remains largely...
Objective:
To examine the effects and mechanisms of AGAP2 Antisense RNA 1 (AGAP2-AS1) in progression of skin cutaneous melanoma (SKCM).
Methods:
AGAP2-AS1 expression and SKCM survival outcomes were assessed using bioinformatics analysis. In vitro and in vivo assays, including cell proliferation, colony formation, migration, and tumor formation a...
Breast cancer is among the most common malignant cancers in women. B-cell-specific Moloney murine leukemia virus integration site 1 (BMI-1) is a transcriptional repressor that has been shown to be involved in tumorigenesis, the cell cycle, and stem cell maintenance. In our study, increased expression of BMI-1 was found in both human triple negative...
Background:
The function of Asb15, which encodes an ASB protein with ankyrin (ANK) repeats and a C-terminal suppressor of cytokine signaling (SOCS) box motif, in male germ cells is poorly understood. Because expression of Asb15 is enriched in mouse testis, it may have a role in spermatogenesis.
Methods and results:
We used a computer-assisted sp...
Background
Spermatogenesis accompanied by self-renewal and differentiation of spermatogonia under complicated regulation is crucial for male fertility. Our previous study demonstrated that the loss of the B-lymphoma Mo-MLV insertion region 1 (BMI1) could cause male infertility and found a potential interaction between BMI1 and proline-rich protein...
Hepatocellular carcinoma (HCC) has a poor prognosis because of its limited drug responses in clinical trials. Therefore, it is crucial to clarify the molecular mechanisms of HCC progression to identify new diagnostic markers and therapeutic targets. Here, we report that brachyury, which regulates the gene encoding the non-SMC condensin II complex s...
Background
Interactions between Sertoli and germ cells are essential for spermatogenesis and male fertility. However, the mechanism of action underlying these interactions in the testes remains largely unknown. In this study, we investigated the distribution and function of syntaxin binding protein 2 (STXBP2) in the mammalian testis.
Methods and r...
Background
Stem cell niche maintains stem cell population identity and is essential for the homeostasis of self-renewal and differentiation in Drosophila testes. However, the mechanisms of CySC lineage signals-mediated soma–germline communications in response to external stimuli are unclear.
Methods
Pre-initiation complex functions were evaluated...
As the main component of seminiferous tubules, Sertoli cells are in close contact with germ cells and generate niche signals, which exhibit pivotal functions in spermatogenesis and male fertility. However, the regulatory mechanisms of Sertoli cell–germline interactions (SGIs) in the testes of neonatal mice (NM) remain largely unclear. Previously, w...
Background
Spermatogonial stem cells (SSCs) are unique stem cells that account for the whole reproductive life of males and transmit genetic information to offspring. SSC maintenance is intricate and the underlying mechanisms are largely unclear. Here, we report that SSC maintenance is driven by the plasminogen receptor (PLGRKT).
Methods and resul...
Objectives:
Studies have demonstrated that B lymphoma Mo-MLV insertion region 1 (BMI1) plays an important role in male reproductive function and the regulation of spermatogonia proliferation. However, whether BMI1 exerts a similarly important function in spermatocyte development remains unclear.
Methods:
In this study, we investigated the role o...
Objective
Long non-coding (lnc) RNAs are critical regulators in carcinogenesis. The novel lncRNA DEPDC1 antisense RNA 1 ( DEPDC1-AS1) was recently associated with poor prognosis in triple-negative breast cancer and lung adenocarcinoma. However, its role in regulating the malignant progression of gastric cancer (GC) and its molecular mechanism are u...
The self-renewal of spermatogonial stem cells (SSCs) requires a special microenvironment and is strictly controlled. Previously, we identified BMI1 as a key regulator of spermatogenesis in a knock-out mouse model. However, the mechanisms by which BMI1 regulates SSC maintenance remain largely unknown. Herein, we show that BMI1 is essential for SSC m...
Background
Osteosarcoma (OS) is the most common malignant tumor of bone, and the clinical efficacy of current treatments and associated survival rates need to be further improved by employing novel therapeutic strategies. Although various studies have shown that BMI1 protein is universally upregulated in OS cells and tissues, its specific role and...
Background:
A major goal of spermiation is to degrade the apical ectoplasmic specialization (ES) junction between Sertoli cells and elongating spermatids in preparation for the eventual disengagement of spermatids into the lumen. E3 ubiquitin ligases mediate the process of ubiquitination and the subsequent proteasomal degradation, but their specif...
Spermatogonia are accountable for spermatogenesis and male fertility, but the underlying mechanisms involved in spermatogonia maintenance are not clear. B lymphoma Mo-MLV insertion region 1 (BMI1) is a key component of epigenetic silencers. BMI1 is essential for stem-cell maintenance. Here, we attempted to uncover the role of BMI1 in spermatogonia...
Spermatogonia transit-amplifying (TA) divisions are crucial for the differentiation of germline stem cell daughters. However, the underlying mechanism is largely unknown. In the present study, we demonstrated that CG6015 was essential for spermatogonia TA-divisions and elongated spermatozoon development in Drosophila melanogaster. Spermatogonia def...
Testosterone biosynthesis progressively decreases in aging males primarily as a result of functional changes to Leydig cells. Despite this, the mechanisms underlying steroidogenesis remain largely unclear. Using gene knock-out approaches, we and others have recently identified Bmi1 as an anti-aging gene. Herein, we investigate the role of BMI1 in s...
Stem cell niche is regulated by intrinsic and extrinsic factors. In the Drosophila testis, cyst stem cells (CySCs) support the differentiation of germline stem cells (GSCs). However, the underlying mechanisms remain unclear. In this study, we found that somatic CG6015 is required for CySC maintenance and GSC differentiation in a Drosophila model. K...
Meiotic homologous recombination (HR) initiates with the programmed generation of DNA double-strand breaks (DSBs), which result in the exchange of genetic information and genome diversity. This process requires the tight cooperation of the MRE11-RAD50-NBS1 (MRN) complex to promote DSB formation and DNA end resection. However, the mechanism regulati...
Background
Retinoic Acid Induced Protein 14 ( Rai14 ) is an evolutionarily conserved gene that is highly expressed in the testis. Previous experiments have reported that small interfering RNA (siRNA)-mediated gene knockdown (KD) of Rai14 in rat testis disrupted spermatid polarity and transport. Of note, a gene knockout (KO) model is considered the...
Background:
Members of the ankyrin repeat and SOCS box (Asb) family are expressed abundantly in testes. Some Asb genes/proteins are required for spermatogenesis, but the function of Asb12 during spermatogenesis is not clear. We investigated the physiological role of Asb12 in murine testes.
Methods:
The clustered regularly interspaced short palin...
Meiosis is a highly conserved process, and is responsible for the production of haploid gametes and generation of genetic diversity. We previously identified the transferrin receptor (TFRC) in the proteome profile of mice neonatal testes, indicating the involvement of the TFRC in meiosis. However, the exact molecular role of the TFRC in meiosis rem...
Objectives:
Stem cell niche regulated the renewal and differentiation of germline stem cells (GSCs) in Drosophila. Previously, we and others identified a series of genes encoding ribosomal proteins that may contribute to the self-renewal and differentiation of GSCs. However, the mechanisms that maintain and differentiate GSCs in their niches were...
In males, aging is accompanied by decline in serum testosterone levels due to impairment of testicular Leydig cells. The polycomb protein BMI1 has recently been identified as an anti-aging factor. In our previous study, BMI1 null mice showed decreased serum testosterone and Leydig cell population, excessive oxidative stress and p16/p19 signaling ac...
Myotubularin related protein 7 (MTMR7), a key member of the MTMR family, depicts phosphatase activity and is involved in myogenesis and tumor growth. We have previously identified MTMR7 in the proteomic profile of mouse spermatogonial stem cell (SSC) maturation and differentiation, implying that MTMR7 is associated with neonatal testicular developm...
The nuclear pre-mRNA spliceosome is a large complex containing five small nuclear ribonucleoprotein particles (snRNPs) and many splicing factors. Messenger RNAs (mRNAs) are generated from pre-mRNAs by the process of RNA splicing, which is conserved in eukaryotes. Precursor RNA processing 3 (Prp3) is a U4/U6-associated snRNP whose function remains l...
The aim of the present study was to identify predictive factors for cervical cancer (CC) progression using a multistage approach. The present study obtained data from 390 healthy women and 259 patients with cervical cancer between June 2012 and June 2017, and used a multiple stage re-analysis strategy for clinical detection of CC. A total of seven...
Background:
Sequencing cell-free DNA in maternal plasma is an effective noninvasive prenatal testing technique that has been used in fetal aneuploidy screening worldwide. However, its clinical application is limited by the low fetal fraction (<4%) of cell-free DNA in many singleton pregnancies, which usually results in screen failures or no calls....
The ribonucleoprotein (RNP) spliceosome machinery triggers the precursor RNA splicing process in eukaryotes. Major spliceosome defects are implicated in male infertility; however, the underlying mechanistic links between the spliceosome and the ribosome in Drosophila testes remains largely unresolved. Small ribonucleoprotein particle protein SmD3 (...
Self-renewal and differentiation in germline stem cells (GSCs) are tightly regulated by the stem cell niche and via multiple approaches. In our previous study, we screened the novel GSC regulatory gene Srlp in Drosophila testes. However, the underlying mechanistic links between Srlp and the stem cell niche remain largely undetermined. Here, using g...
Germ cell maturation is essential for spermatogenesis and testis homeostasis. ATP synthase serves significant roles in energy storage in germ cell survival and is catalyzed by alterations in the mitochondrial membrane proton concentration. The intrinsic cellular mechanisms governing stem cell maturation remain largely unknown. In the present study,...
The incidence of fertile women with missed abortion dramatically increased in recent years, while very few serum indices have been identified for the diagnosis of missed abortion. The aim of this study was to identify related factors for missed abortion through a retrospective study of serum indices.
A total of 795 cases of women with missed aborti...
Study question:
What is the function of CAV1 in folliculogenesis and female reproduction?
Summary answer:
CAV1 regulates germline cyst breakdown and primordial follicle (PF) formation in mice, and CAV1 mutation may be related to premature ovarian insufficiency (POI).
What is known already:
Pre-granulosa cells are essential for the establishmen...
While it is known that spermatogonial stem cells (SSCs) initiate the production of male germ cells, the mechanisms of SSC self-renewal, proliferation, and differentiation remain poorly understood. We have previously identified Strawberry Notch 1 (SBNO1), a vertebrate strawberry notch family protein, in the proteome profile for mouse SSC maturation...
Stromal interaction molecule 1 (STIM1) is a transmembrane endoplasmic reticulum protein, and it serves as a Ca2+ sensor and activator of store-operated Ca2+ entry (SOCE). We have previously identified STIM1 in the proteome profile of mice neonatal testes, revealing STIM1 to be associated with neonatal testicular development. Here, to further explor...
Background:
Non-invasive prenatal testing (NIPT) has been widely used for detecting fetal trisomy 13, 18 and 21 and sex chromosome aneuploidies (SCAs), but few studies have been published comparing the performance of NIPT for fetal SCAs between different next-generation sequencing platforms.
Method:
Retrospective analysis of prospectively collec...
Objective:
To explore the cause of failure of non-invasive prenatal testing (NIPT) using cell-free fetal DNA from peripheral maternal blood.
Methods:
A total of 31 832 cases of NIPT were retrospectively analyzed. The clinical data of pregnant women were analyzed and the outcome of pregnancy was followed up.
Results:
Among the 31 832 cases, 200...
The cellular nucleic acid-binding protein (CNBP), also known as zinc finger protein 9 (ZNF9), is a highly conserved zinc finger protein that is strikingly conserved among vertebrates. Data collected from low vertebrates showed that CNBP is expressed at high levels and distributed in the testes during spermatogenesis. However, the location and funct...
The early stage of embryogenesis is an important and complex cell-remodeling event in reproductive biology. To develop into a normal zygote, maternal-to-zygotic transition (MZT) is especially important for both zygotic genome activation (ZGA) and degradation of maternal products during the early stage of embryonic development. β-Catenin has been id...
Generation of haploid gametes by meiosis is a unique property of germ cells and is critical for sexual reproduction. Leaving mitosis and entering meiosis is a key step in germ cell development. Several inducers or intrinsic genes are known to be important for meiotic initiation, but the regulation of meiotic initiation, especially at the protein le...
Osteoarthritis (OA) is a complex disorder characterized by degenerative articular cartilage and is largely attributed to genetic risk factors. Single nucleotide polymorphisms (SNPs) are common DNA variants that have shown promising and efficiency, compared with positional cloning, to map candidate genes of complex diseases, including OA.
In this st...
To detect complex fetal subchromosomal abnormalities by noninvasive prenatal testing (NIPT).
Case presentation
After routine prenatal serum screening, the plasma of high-risk pregnant women were tested via NIPT, and the NIPT results were further validated by fetal karyotype analysis and array-based comparative genomic hybridization (aCGH) through a...
Primordial germ cells (PGCs) are embryonic founders of germ cells that ultimately differentiate into oocytes and spermatogonia. Embryonic proliferation of PGCs starting from E11.5 ensures the presence of germ cells in adulthood, especially in female mammals whose total number of oocytes declines after this initial proliferation period. To better un...
Testicular cord formation in male gonadogenesis involves assembly of several cell types, the precise molecular mechanism is still not well known. With the high-throughput quantitative proteomics technology, a comparative proteomic profile of mouse embryonic male gonads were analyzed at three time points (11.5, 12.5 and 13.5 days post coitum), corre...
Citations
... Transfection of shRNA was performed with Lipo2000 (Invitrogen, Carlsbad, USA) according to the manufacturer's instructions, following a previously established protocol. [25][26][27] OVCAR3 cells, which had been genetically modified with short hairpin (sh)-TRIM8 or a sh-negative control (NC), were rinsed twice with phosphate-buffered saline (PBS) and then treated with trypsin for digestion. Finally, the cells were resuspended in PBS at 3 × 10 7 cells/ mL. ...
... The immunoprecipitation products were detected by LC/ MS, as previously described. 28,29 ...
... According to a recent study, the authors used IT-AuNP injection, but AuNPs could not penetrate through the blood-testis barrier (BTB), and no reproductive toxicity was observed in mice. However, when they used RT-AuNP injection, AuNPs were transferred from the adluminal to the interstitial compartment via Sertoli cells, resulting in testicular damage and inflammatory response [163]. ...
... In single cell RNA-seq studies characterizing human and Drosophila spermatogenesis, it was discovered that male germ cell migration and spermatid elongation heavily rely on oxidative phosphorylation and mitochondrial ATP generation. 5, 38 We also discovered that migration phase spermatocytes possessed upregulated ATP synthesis-related genes. This may be connected to the fact that in early gametogenic gonads, a small number of spermatocytes migrating toward the gonadal tubules are accompanied by the process of spermatocyte formation. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/277894103355394-1443266525122_Q64/Fei-Sun-14.jpg)
... Approval was received from the Research Ethics Committee of Suzhou Municipal Hospital. Collected tissues were stored in RNA Keeper Tissue Stabilizer (Vazyme, Nanjing, China) at −80°C as previously described [18]. ...
... [27] Transcription of NCAPG2 promotes the proliferation and migration of liver cancer cells. [28] NCAPG2 is associated with the tumor immune-suppressive microenvironment. Therefore, NCAPG2 may play an important role in the pathological progression of colorectal cancer and gastric cancer. ...
... The colonies were then observed and quantified using a brightfield microscope (Carl Zeiss, Oberkochen, Germany), following a previously established protocol. 23,24 ...
... Drosophila oo18 RNA-binding protein (Orb) and Orb2 are two highly conserved RNA-binding proteins belonging to the cytoplasmic polyadenylation element binding (CPEB) protein family, which plays a critical role in mRNA transport and local translation [55]. Both Orb and Orb2 are highly enriched in elongated spermatids and regulate spermatogenesis [22,56]. Immunofluorescence staining in the Drosophila testis shows that Orb and Orb2 proteins have low levels of expression in mitotic spermatogonia and extremely high levels in elongated spermatids. ...
... The docking of NRSN2-AS1 bound to PTK2 were predicted with HDOCK (http:// hdock.phys.hust.edu.cn/) and visualized with PyMOL (https://pymol.org/ 2/) as previously described [16,17]. The cut-off employed to dichotomize OC patients in high and low NRSN2-AS1 expression is median. ...
... After a duration of 4 h, the microplate reader (Bio-Rad Model 680, Richmond, CA, USA) was employed to measure the absorbance at 450 nm for each well, following the established protocol. 21,22 In addition, colony formation tests were performed to evaluate cell proliferation capacity. Cells, at a density of 1000 cells per well, were transferred to six-well plates and cultured for 2 weeks. ...
