Colin R. Jefcoate's research while affiliated with University of Wisconsin–Madison and other places

... These enzymes affect multiple biochemical pathways, including those related to hormone synthesis and regulation [18][19][20]; estrogen and iron metabolism in particular are closely related [141,142]. For instance, the Cytochrome-P450 enzymes, which process steroid hormones like estrogen and progesterone, significantly depend on iron [143]. Similarly, iron is indispensable for the enzymes Prolyl Hydroxylase [144] and Lysyl Hydroxylase [145] involved in collagen synthesis [146], thereby influencing the health of the connective tissue in reproductive organs [147]. ...

... Our data are consistent with studies performed in experimental rodent and human liver disease (57)(58)(59), showing that injuryinduced release of Hedgehog ligands from epithelia leads to hepatic myofibroblast activation. Studies in other organs in humans, mice, zebrafish, and fruit flies also support the ability of Hedgehog signaling to drive local and remote tissue development, cancer, and post-injury fibrosis (60)(61)(62)(63)(64)(65). The literature on cardiac Hedgehog signaling has focused mainly on the beneficial impact of Hedgehog signaling in cardiac development and on successful scar formation in the first 7 days after myocardial infarction (66). ...

... This complex is required for the transport of precursor proteins into the mitochondria to maintain mitochondrial function 1 . Furthermore, TOMM40 has been shown to interact with key regulators of mitochondrial function such as BAP31 2 , affect calcium signalling via its interaction with VDAC channels (voltage dependent anion channels) [3][4][5] , and have effects at mitochondria-ER contact sites (MERCs) that impact mitochondrial cholesterol transport via steroidogenic acute regulatory protein (STAR aka STARD1) 6,7 . In mouse Leydig cells, the absence of TOMM40 causes STAR to lose its ability as a cholesterol transporter, depleting mitochondrial cholesterol content 8 . ...

... Гемопоэтические стволовые и прогениторные клетки имеют минимальный набор цитохромов P450. В двух исследованиях было показано присутствие мРНК и белков только цитохромов P450 1B1и 2E1 в CD34+ гемопоэтических стволовых и клетках-предшественниках и отсутствие P4503A4/7 и P450 2C9 [28,29]. На более поздних стадиях дифференцировки и в клетках периферической крови количество экспрессируемых цитохромов Р450 возрастает. ...

... Cyp1a1 deletion has much less of an effect. These Cyp1b1-AhR changes show non-canonical features that could arise from ROS participation, mediated by NF-κB [38][39][40][41] or Nrf2 partnership with AhR [42][43][44][45]. The relationship between Cyp1b1 and AhR in these adherent BM assemblies has been systematically addressed by examining the gene expression effects of in vivo Cyp1 deletions (1a1 -/-, 1b1 -/-, and dual deletions 1a1 -/-;1b1 -/-(DKO)). ...

... This OLFR's presence in both DKO(+) and DKO(-) clusters, as well as in BMS2 cells, suggested expression in lineage-related BM-MSC cells. These GPCRs sense volatile organic molecules generated by microbiome bacteria [13,23,62]. This ectopic expression has been documented in pancreatic islet cells, with appreciable overlap with the present OLFR set (Table S8) [25]. ...

... But the depth of the cavity is non-uniform with this technique [91] . Injection molding has good reproducibility, while the process of the mold is complex [92] . Hot embossing can produce high-aspect-ratio structures, but the process is expensive [93] . ...