Cheryl L Meyerkord's research while affiliated with Emory University and other places

... A parallel assay system was established for this purpose. The uHTS campaign was carried out at the Emory Chemical Biology Discovery Center (ECBDC) [22]. Screening data were analyzed using CambridgeSoft Bioassay software [23]. ...

... TMEM106B has been reported to increase lysosomal hydrolase synthesis in lung cancer cells, which is packaged into lysosomes and enlarge lysosomes (11). A hyperinvasive microenvironment is created as lysosomes secrete their protease cargo into the extracellular matrix under conditions of calcium flux (18)(19)(20). ...

... La BiFC a également l'avantage de pouvoir visualiser les IPP directement in vivo, que ce soit au sein de cellules en culture (Dard et al. 2019), de plantes (Kodama et Wada 2009) ou encore chez la mouche drosophile . Cette technique a permis d'identifier des inhibiteurs d'IPPs, comme par exemple un peptide pouvant perturber la formation d'un complexe oncogène, le dimère PIKE-A/Akt, impliqué dans la survie, l'invasion et la migration des cellules de certains glioblastomes multiformes (Qi et al. 2013). ...

... [20,[22][23][24][25][26] In contrast to plant 14-3-3, a large number of inhibitors and stabilizers for mammalian 14-3-3 have been developed toward cancer and neuropathy therapies. [10,[27][28][29][30][31][32][33][34][35][36] However, it remains very challenging to design isoform-selective modulators that distinguish the highly conserved peptide-binding pocket ( Figure 1). [37][38][39][40] Nevertheless, development of covalent and/or allosteric 14-3-3 binders have provided valuable insight into the isoform-specific modulation. ...

... While many autophagy marker proteins exhibit scattered punctate patterns of subcellular localization, ATG9B exhibited a unique elongated tubular pattern in USCs ( Figure 4). ATG9-positive cytoplasmic structures have been observed in elongated tubular patterns, as Atg9 can be localized to Golgi membranes during fragmentation under serum starvation conditions [25]. Our observations reflect a similar phenomenon in serum-starved USCs. ...

... Protein phase separation forms liquid tissue on the surface of the membrane, which exerts compressive stress on the membrane, thus affecting the curvature of the membrane [16]. Bax interaction factor 1 (Bif-1) was originally identified as a Bax-binding protein, containing N-BAR (Bin-Amphiphysin-Rvs) domain and SH3(SrC-homology 3) domain [17]. As a key regulatory molecule for cell membrane binding and bending activities, Bif-1 could regulate intracellular events by inducing membrane curvature [17,18]. ...

... Given the relatively lower co-localization of endogenous PUMA to the ER marker in MCF-7 cells, we examined localization of endogenous PUMA after the induction of PUMA expression by ER stress (Reimertz et al., 2003;Yu and Zhang, 2008) or genotoxic stress (Meyerkord et al., 2008;Jamil et al., 2015). Previous results suggest that a P53-dependent response to genotoxic stress results resident endoplasmic reticulum membrane markers), Calr-KDEL (recycling between ER and Golgi), ERGIC (ER-Golgi intermediate compartment), GalT (trans-Golgi), Golgin84 (cis-Golgi), MAO (outer mitochondrial membrane), CCO (inner mitochondrial membrane), MAM (mitochondrial associated ER membrane), Rab5A, Rab7A, VAMP2 (transport and secretory vesicles), VAMP5 (Secretory pathway to the plasma membrane), ΔTMD-VAMP1 (cytoplasm), Lamin A (nuclear envelope), PTS1 (peroxisomes), LAMP1 (Lysosomes) (Schormann et al., 2020) in the extension of peripheral tubular ER and promotes the formation of ER-mitochondrial contact sites (Wang et al., 2007) which may be binding sites for PUMA. ...

... Next, we established and tested marker proteins to identify each cell cycle phase of a single cell. This identification can be based on the number of cells in a specific cell cycle phase by protein alterations like translocation (16)(17)(18), differences in expression (17,19,20) and/or changes in the phosphorylation level (14,18,21). Table 1 lists stainings that are commonly used to detect the stage of a cell in the different cell cycle phases via immunofluorescence. ...

... Bax-interacting factor 1 (Bif-1), also known as SH3GLB1 or lipophilic enzyme B1, is present in the cytoplasm, and is a key protein in the process of autophagy and autophagosome formation (19)(20)(21). The loss of Bif-1 may impede the endogenous apoptotic pathway while facilitating tumor development (22,23). Bif-1 exerts inhibitory effects on the production of mitochondrial and glycolytic ATP, and its downregulation contributes to the proliferation of melanoma cells (24). ...