Brad Zerler's research while affiliated with Temple University and other places
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Publications (11)
Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extracellular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extracellular matrix, allowing them to perform their respective functions during osteogenesis. Several other functions have been sugg...
We are using viral oncogene probes to study the pathways by which osteoblast-specific gene expression is induced in ascorbic acid-treated MC3T3-E1 cells. The 12S product of the adenovirus E1A gene binds directly to key cellular regulators and, as a result, represses tissue specific gene expression and blocks differentiation in a wide variety of cel...
We are using viral oncogene probes to study the pathways by which osteoblast-specific gene expression is induced in ascorbic acid-treated MC3T3-E1 cells. The 12S product of the adenovirus E1A gene binds directly to key cellular regulators and, as a result, represses tissue specific gene expression and blocks differentiation in a wide variety of cel...
We are using viral oncogene probes to study the pathways by which osteoblast-specific gene expression is induced in ascorbic acid-treated MC3T3-E1 cells. The 12S product of the adenovirus E1A gene binds directly to key cellular regulators and, as a result, represses tissue specific gene expression and blocks differentiation in a wide variety of cel...
Among the various biological activities expressed by the products of the adenovirus E1A gene are the abilities to induce cellular DNA synthesis and proliferation in quiescent primary baby rat kidney cells. The functional sites for these activities lie principally within two regions of the E1A proteins: an N-terminal region and a small second region...
We have analyzed the cell cycle effects that different domains of the adenovirus E1A proteins have on quiescent primary BRK cells. Studies with deletion mutants that in combination removed all but the N-terminal 85 amino acids common to both the 12S and 13S proteins suggest that this region may be sufficient for the induction of synthesis of prolif...
The transformation and early adenovirus gene transactivation functions of the E1A region were analyzed with deletion and point mutations. Deletion of amino acids from position 86 through 120 had little effect on the lytic or transforming functions of the E1A products, while deletion of amino acids from position 121 through 150 significantly impaire...
Plasmids expressing partial adenovirus early region 1A (E1A) coding sequences were tested for activities which facilitate in vitro establishment (immortalization) of primary baby rat kidney cells and which enable the T24 Harvey ras-related oncogene and the polyomavirus middle T antigen (pmt) gene to transform primary baby rat kidney cells. E1A cDNA...
Citations
... [101,102] In addition, SV40 tag can enhance transcription from E2F-activated promoters of early growth response genes. [103,104] The process of rodent cell transformation induced by SV40 typically depends on the integration of the viral DNA into the host genome where it produces a high level of expression of the major viral oncogenic proteins, Tag, and tag. However, human cells experimentally transformed by SV40 harbor viral genomes in an episomal state in addition to integrated viral DNA. ...
... Pi groups with calcium ions further deposit amorphous calcium phosphate or hydroxyapatite crystals within the secreted ECM (Vimalraj, 2020;Ansari et al., 2022). This is considered as an early phase marker of bone tissue formation, while at later phases ALP expression reaches a peak (plateau) value and finally decreases indicating the mineralisation of ECM (Choi et al., 1996;Beck et al., 1998;St-Pierre et al., 2005). This pattern was observed only in the d7 scaffolds (Figure 9), while the d3 scaffolds exhibited a continuously increasing activity of the enzyme suggesting either lag in cell differentiation or a state of continuous ECM mineralisation. ...
... This biological activity of the ANK domain was speci®c for Notch-IC since other ankyrin repeat containing proteins like IkappaB could not substitute for Notch-IC in this assay (data not shown). All results were con®rmed by testing MTE1A cells, which express E1A under the control of the lothionin promoter (Zerler et al., 1986). In order to exclude speci®c contributions of the cellular background of the RK3E cells, the Notch-IC, the RAMANK and the ANK fragments were tested for RBP-J dependent transcription in these cells (Figure 4b). ...
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... Alkaline phosphatase is the most widely recognized early marker of osteoblast differentiation (Beck et al., 1998), as a typical protein product of the osteoblast phenotype and osteoblast differentiation. (TA@HA/Lys) n coatings have an osteogenic mineralization effect on MC3T3-E1 cells, and DPSCs have the ability to differentiate into osteoblasts as well. ...
... Col I-and PLL-coated plates, which have high biocompatibility, were used to clarify the effect of different culture conditions on biomaterial evaluation using MC3T3-E1 cells. Early ALP activity of the cells reportedly increases over time and then plateaus in many cases [5,28,29]. Measurements on day 7 in this study show the accelerating effect of the coating molecules on ALP activity. In this study, Col I, which is known to promote osteogenesis in MC3T3-E1 cells, required an αMEM-based medium supplemented with fresh VC to increase ALP activity on normal plates. ...
... These results indicate that phosphate is a signaling molecule. Several studies showed that high extracellular phosphate activates the extracellular signal-regulated kinase (ERK) kinase (MEK)/ERK pathway [35][36][37][38][39]. Our research group has also reported that high extracellular phosphate induced the phosphorylation of ERK, and induced the expression of several transcription factors downstream in the MEK/ERK pathway by transcriptome analysis in in vitro experiments [13]. ...
