Bo Zhao's research while affiliated with Changhai Hospital, Shanghai and other places

There has been few research on the affect and distribution of different shapes of nanoparticles inside an organism during extraction and drug targeting. In order to obtain the distribution of magnetic nanoparticles with different morphology and size in vivo, a general method of Re-188 labeled Magnetic Core–Shell Nanoparticles (MNPs) Materials was developed. Based on the prepared magnetic particles with three different morphologies and sizes, including 230 nm spherical Fe3O4@SiO2 particles (S-230), 100 nm spherical Fe3O4@SiO2 particles (S-100) and peanut shaped Fe3O4@SiO2 particles (P-180, the length of the short axis is about 100 nm and the length of the long axis is about 180 nm), the aminated MNPs were labeled with radionuclide Re-188 through the coupling of diethylenetriamine pentaacetic anhydride. The nuclide Re-188 was labeled to investigate their distribution behavior in mice. Most of the small-size particles S-100 can be separated from the capture of the endothelial reticular system and removed by renal metabolism. Most of the larger particles, S-230 and P-180, will be captured by the endothelial reticular system, and the nanoparticles P-180 with large aspect ratio are easier to be captured by the tissue in the spleen and enter the cells through endocytosis.

There has been few research on the affect and distribution of different shapes of nanoparticles inside an organism during extraction and drug targeting. In order to obtain the distribution of magnetic nanoparticles with different morphology and size in vivo, a general method of Re-188 labeled Magnetic Core-Shell Nanoparticles (MNPs) Materials was developed. Based on the prepared magnetic particles with three different morphologies and sizes, including 230 nm spherical Fe 3 O 4 @SiO 2 particles (S-230), 100 nm spherical Fe 3 O 4 @SiO 2 particles (S-100) and peanut shaped Fe 3 O 4 @SiO 2 particles (P-180,the length of the short axis is about 100 nm and the length of the long axis is about 180 nm),the aminated MNPs were labeled with radionuclide Re-188 through the coupling of diethylenetriamine pentaacetic anhydride (DTPAA). The nuclide Re-188 was labeled to investigate their distribution behavior in mice. Most of the small-size particles S-100 can be separated from the capture of the endothelial reticular system and removed by renal metabolism. Most of the larger particles, S-230 and P-180, will be captured by the endothelial reticular system, and the nanoparticles P-180 with large aspect ratio are easier to be captured by the tissue in the spleen and enter the cells through endocytosis.

Hydrophilic mesoporous organosilica nanoparticles (HMNPs) were synthesized by introducing Fe3O4 on the basis of mesoporous organosilicon nanoparticle hollow spheres as a drug carrier. N- and S- doped graphene quantum dots (NS-GQDs) and polyethylenimine (PEI) were grafted onto the surface of HMNPs. Finally, two types of composite mesoporous particles, HMNPs-NS-GQDs-PEI-HA and HMNPs-NS-GQDs-PEI-FA which can enter VX2 cells for fluorescence confocal imaging, were further modified by targeting ligand hyaluronic acid (HA) and folic acid (FA). The heteroatoms of NS-GQDs have intelligent functional effects, which can not only improve the photoluminescence performance of GQDs, but also generate conjugated quantum dots with GA, so as to synergistically improve the drug effectiveness. Under illumination by near-infrared (NIR) radiation, gambogic acid (GA)-loaded HMNPs was promoted to the sustained release of gambogic acid. The loading and sustained release of the anticancer drug GA on the compound mesoporous particles were studied. The cytotoxicity and antitumor properties of the compound mesoporous particles were investigated by both in vitro cell experiments and in vivo experiments in mice. The results showed that HMNPs-NS-GQDs-PEI-HA and HMNPs-NS-GQDs-PEI-FA have desired fluorescence and magnetic properties for cell fluorescence imaging and magnetic targeting of cancer cells. Both of composite mesoporous particles have desired loading capacity on GA and pH-responsive drug release behavior as well as low cytotoxicity and good biocompatibility. The cell survival rate of HMNPs-NS-GQDs-PEI-HA and HMNPs-NS-GQDs-PEI-FA loaded with GA was only 7.69% and 8.71%, respectively, after 24 h, which can effectively induce the apoptosis of VX2 cells. Tumor experiments in mice demonstrated that HMNPs-NS-GQDs-PEI-HA inhibits tumor growth and exhibits magnetic targeting function.