B.C. Hatapakki's scientific contributions

The ethanol and aqueous extracts of leaves of Neptunia Oleracea lour (Mimosaceae) were tested for their efficacy against carbon tetrachloride (CCl4) induced hepatotoxicity in Wistar albino rats. The different groups of animals were administered ethanol and aqueous extracts to carbon tetrachloride treated rats. In the present study ethanol extract (p<0.01) and aqueous extract (p<0.05) significantly decreases the level of alanine aminotransferase, aspartase aminotransferase, alkaline phosphatatase, total bilirubin and direct bilirubin in blood whereas total protein level is elevated. The phytochemical screening revealed the presence of active phytoconstituents i.e. flavonoids, triterpinoids and tannins, which may offer hepatoprotection. The present work support the traditional claim of plant in the treatment of liver injury, may provide a new drug against a war with liver diseases.

Objective: To evaluate the anticonvulsant effect of Cyperus rotundus Linn rhizomes against maximal electroshock (MES) and pentylenetetrazole (PTZ) induced tonic seizers in albino rats. Materials and methods: Ethanol extract of Cyperus rotundus Linn rhizomes were obtained by continuous hot soxhlet extraction. The extract was assessed for anticonvulsant activity by measuring (sec) the duration of tonic flexion, tonic extensor, clonus, stupor and recovery phase in rats. Results: Phytochemical investigation of ethanol extract revealed the presence of flavonoids, vitamins and carbohydrates. The ethanol extract (100 mg / kg, p.o.) reduced hind limb extension and duration of convulsion significantly, (P < 0.001) which was comparable to standard drug phenytoin (25 mg / kg, i.p.) and diazepam (4 mg /kg, i.p.) respectively. Conclusion: The ethanol extract of Cyperus rotundus rhizomes is worthwhile to develop the potent phytoconstituent for treatment of epilepsy and the flavonoids present in ethanol extract could be attributed for anticonvulsant activity.

For site-specific one-time controlled delivery of ornidazole, an antimicrobial compound with excellent activity against anaerobic microorganism was studied in the treatment of periodontal disease. Periodontal disease which can and do occur in all groups, ethnicities, races, genders and socioeconomic level associated with periodontium. The drug device which accommodate perfectly into the specified periodontal pocket (6-8mm) with very low loading of 1mg drug was prepared by solvent casting technique using ethyl cellulose, hydroxypropylcellulose, hydropropylmethylcellulose K4M and Eudragit RL-100 with dibutylphthalate as plasticizer. The physicochemical parameters like thickness, weight variation, content uniformity, and release characteristics were evaluated. The drug release was initially high on day one to achieve immediate therapeutic level of drug in periodontal pocket followed by marked fall in release by day two with progressive moderate release profile to maintain therapeutic level following anomalous transport mechanism. Formulation (F4) containing mixture of ethyl cellulose (800mg) and eudragit RL-100 (25mg) released 97.07% of drug at the end of 120 h and was considered as best formulation. Clinical study was carried out on above formulation in which Plaque index, Gingival index, Bleeding index and Probing depth were performed. Results indicated very good improvement which was equally effective as conventional treatment (7.2 gm) of drug and patient compliance was also very high.

Objective: To evaluate ethanol extract of pods of Cassia auriculata Linn at different doses (100, 200 mg/kg, p.o.) for laxative activity in rats. Materials and Methods: The ethanol extract of pods of Cassia auriculata was obtained by continuous soxhlet extraction and further assessed for laxative activity by charcoal meal test and feacal output in experimental rats and compared with standard senna. The extract was subjected to qualitative chemical analysis to identify the phytoconstituents. Results: Upon evaluation of Laxative activity on experimental animals, the ethanol extract at the dose 200 mg/kg, p.o. showed significant (P<0.001) laxative activity as observed from different evaluation parameters. However, the same at the dose 100 mg/kg, p.o. was less significant. The ethanol extract showed the presence of flavonoids, triterenoids, tannins, sterols and anthracene derivatives. Conclusion: From the results, it is revealed that, the active ethanol extract (200 mg/kg, p.o.) of Cassia auriculata pods is worthwhile to develop the bioactive principle for laxative activity and it is also concluded that, the anthracene derivatives present, could be attributed for the laxative activity.

Objective: To evaluate the antidiabetic activity of flowers of Cassia auriculata Linn against alloxan-induced diabetes in rats and to isolate the bioactive constituents from the activity guided fraction. Materials and methods: The ethanol & aqueous extracts of flowers of Cassia auriculata were obtained by continuous soxhlet extraction & cold maceration respectively. Each extract was assessed for antidiabetic activity by estimating serum glucose level in diabetic rats. The active ethanol extract was subjected to qualitative chemical analysis to identify the active phytoconstituents. The sterol; β sitosterol, was isolated from ethanol extract by column chromatography. Results: In alloxan-induced diabetic rats the ethanol extract (250 mg/kg, p.o.) showed significant (P<0.001) antidiabetic activity as observed from serum glucose level in diabetic rats. However, the aqueous extract (250 mg/kg, p.o.) did not significantly reduce the serum glucose level in diabetic rats. The ethanol extract showed the presence of sterols, triterpenoids, flavonoids and tannins. Conclusion: From the results, it is revealed that, the active ethanol extract of Cassia auriculata flowers is worthwhile to develop the bioactive principle for diabetes mellitus and it is also concluded that the isolated sterol; β sitosterol, could be attributed for antidiabetic activity.