Andrew A. Pascal's research while affiliated with Université Paris-Saclay and other places

... ; https://doi.org/10.1101/2024.03.20.585890 doi: bioRxiv preprint as from each other 35,36 . The ν4 band located around 960 cm -1 arises from C-H out-of-plane wagging motions coupled with C=C torsional modes (out-of-plane twists of the carbon backbone) 33 . ...

... Whereas there is a lively debate around the SF mechanism and the role of intermediate species 7,8,9,10,11,12,13,14 , there have been no major advances in the application of SF to improve photocatalytic e ciencies. Indeed, the current scarcity of feasibility studies for SF in aqueous solution is remarkable, with only a few reports involving aqueous suspensions of diketopyrrolopyrrole nanoparticles 15 or carotenoid aggregates 16,17,18 . Moreover, none of these involve molecular systems in solution, which would simplify their coupling with molecular photocatalysts signi cantly. ...

... [32,33], Sargassum angustifolium [34], Turbinaria turbinata [35], etc. Additionally, siphonaxanthin, a particular keto-carotenoid, is accumulated in certain green seaweed [36,37], such as Codium cylindricum [38,39], Codium fragile [36,37,40,41], Halimeda sp., [42], etc. The sources of three biopigments from various types of seaweed are presented in Table 1. ...

... These conformational changes illustrate the flexibilities of trimeric Bry-LHCII, which may be associated with its functional transformation between light harvesting and energy quenching. 20,42 In analogy with the Spi-LHCII, the crystal structure of Bry-LHCII may represent an aggregated state which presents stronger quenching features, 39 whereas its cryo-EM structure may represent a solubilized state, so the differences between the crystal and cryo-EM structures may represent the quenched and light-harvesting states, respectively. On the other hand, our structures showed little changes of Chls and Cars (Figures 5 and S7) between the two Bry-LHCII structures, this could be due to limitations in the resolution. ...

... The cultures were centrifuged and collected cells were resuspended in induction buffer (10 mM MgCl 2 , 10 mM MES, 150 μM acetosyringone, pH=5.6) to achieve OD 600 = 4. The mixture was incubated at room temperature for 3 h and then mixed with the same volume of a culture harboring vector pGWB702-HCProWMV at the same OD to prevent silencing 49 and induction buffer for a final OD = 0.5 for each strain. Mixtures were infiltrated in two different leaves of 5-10 3-4-week-old N. benthamiana plants grown under normal greenhouse conditions as described 49 . ...

... It has been widely assumed that heme binds to the majority of heme proteins, such as the globins and most of the catalytic heme enzymes, with high or relatively high affinity and, therefore, irreversibly. But the logic of heme also acting as a biological regulatorfor which there is now ample evidence (12)(13)(14)(15)(16)(17)(18)(19)(20) does not fit with this concept, as heme may need to bind transiently and reversibly in order to exert any regulatory role. A number of heme-regulated cellular functions have now been uncovered where low affinity, presumed to be reversible, heme binding more sensibly accounts for activation/inactivation of cellular pathways (21)(22)(23)(24)(25)(26). ...

... ; https://doi.org/10.1101/2024.03.20.585890 doi: bioRxiv preprint as from each other 35,36 . The ν4 band located around 960 cm -1 arises from C-H out-of-plane wagging motions coupled with C=C torsional modes (out-of-plane twists of the carbon backbone) 33 . ...

... As a result, the conserved Asn in FRL-AP is instead found as a surface residue, ~ 12 Å away from the α-chromophore. Thus, unlike the α-chromophores of AP and AP-B, pyrrole ring A is not stabilized by H-bonding from α-Asn71 in FRL-AP, which is interesting because H-bonding interactions are frequently observed to red shift the absorbance of cyclic tetrapyrroles (e.g., (Llansola-Portoles et al. 2020)). Residue α-Asn71 in FRL-AP is therefore conserved in sequence but not structure or function. ...

... Mixed bands of lipids and proteins and proteins and nucleic acids can be seen at 1,337 and 935 cm 21 , respectively. In the mean spectrum, the characteristic spectral pattern of carotenoids can be observed at 1,523, 1,160, and 1,007 cm 21 (29,31) that represent the most prominent peaks in the difference spectrum. These signals derive mainly from the carotenoid staphyloxanthin, the characteristic golden pigment of S. aureus present in the majority of the strains of this species (32,33). ...

... Indeed, this abnormal ratio has already been observed for Vau when comparing its 77 K resonance Raman spectra with those of diadinoxanthin and heteroxanthin ( Figure S1). 40 In Raman spectra of Vio in n-hexane, the ν 2 region displays a single main peak at 1158 cm −1 , together with two satellite bands at 1192 and 1215 cm −1 . This region constitutes a fingerprint for the carotenoid isomerization state (cis/ trans), 37−39 and such a profile unambiguously indicates that violaxanthin is in the all-trans configuration. ...