Akira Watanabe's research while affiliated with Kagawa University and other places
What is this page?
This page lists the scientific contributions of an author, who either does not have a ResearchGate profile, or has not yet added these contributions to their profile.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
Publications (23)
The biochemical mechanism underlying the development of fruiting bodies in Flammulina velutipes, an edible mushroom, was investigated using the YBLB colorimetric assay to distinguish between the normal strain (FVN-1) and the degenerate strain (FVD-1). In this assay, the color of the YBLB medium (blue-green) inoculated with FVN-1 exhibiting normal f...
Extracellular laccase isozyme (FvLcc3) from the edible mushroom Flammulina velutipes was found to be undetectable under the culture condition for fruiting body formation. FvLcc3 was purified and determined to be an approximately 53-kDa monomeric protein. FvLcc3 showed the highest catalytic efficiency (kcat/Km) toward 2,2'-azino-bis(3-ethylbenzothia...
Some rare sugars can be potently medicinal, such as l-gulose. In this study, we present a novel alditol oxidase (fAldOx) from the soil fungus Penicillium sp. KU–1, and its application for the effective production of l-gulose. To the best of our knowledge, this is the first report of a successful direct conversion of d-sorbitol to l-gulose. We furth...
We found that l-gulose, a rare sugar, was produced from d-sorbitol efficiently, using a wheat-bran culture extract of the fungus Penicillium sp. KU-1 isolated from soil. The culture extract showed enzyme activity for the oxidation of d-sorbitol to produce l-gulose; a high production yield of approximately 94% was achieved.
Two cDNAs encoding the minor laccase isozymes (Lac2 and Lac3) of Grifola frondosa were cloned, characterized, and expressed in Pichia pastoris. The recombinant Lac2 (rLac2) was stable at pH 6.0, whereas the recombinant Lac3 (rLac3) was stable in a broad pH range (pH 4.0-8.0). In addition, rLac2 and rLac3 showed the highest catalytic efficiency (kca...
Here, we report the occurrence of the (2R,3S)-isomer of 2-amino-3,4-dihydroxybutanoic acid (D-ADHB) in the fruiting body of an edible mushroom, Hypsizygus marmoreus. This is an unusual example of the accumulation of a D-amino acid, whose enantiomer is not a proteinogenic amino acid. We show that D-ADHB occurs specifically in the mushroom H. marmore...
Laccase from Trametes polyzona WR710-1 was produced under solid-state fermentation using the peel from the Tangerine orange (Citrus reticulata Blanco) as substrate, and purified to homogeneity. This laccase was found to be a monomeric protein with a molecular mass of about 71 kDa estimated by SDS-PAGE. The optimum pH was 2.0 for ABTS, 4.0 for L-DOP...
Purified laccase from Trametes polyzona WR710-1 was used as biocatalyst for bisphenol A biodegradation and decolorization of synthetic dyes. Degradation of bisphenol A by laccase with or without redox mediator, 1-hydroxybenzotriazole (HBT) was studied. The quantitative analysis by HPLC showed that bisphenol A rapidly oxidized by laccase with HBT. B...
A major laccase isozyme from Grifola frondosa (Lac 1) was found to be effective for decolorizing of synthetic dyes and degrading of bisphenol A. The oxidative capability of Lac 1 toward synthetic dyes and bisphenol A was enhanced in the presence of the redox mediator, 1-hydroxybenzotriazole. The major product from the degradation of bisphenol A by...
In a previous study, we conducted an expression cloning screen of a cDNA library prepared from Coprinopsis cinerea mycelia using Multi-PK antibodies and detected a wide variety of Ser/Thr protein kinases. One of the isolated clones, CMZ032, was found to encode a putative Ser/Thr protein kinase designated CoPK32. In the present study, we investigate...
A major laccase isozyme (Lac 1) was isolated from the culture fluid of an edible basidiomycetous mushroom, Grifola frondosa. Lac 1 was revealed to be a monomeric protein with a molecular mass of 71 kDa. The N-terminal amino acid sequence of Lac 1 was highly similar to those of laccases of some other white-rot basidiomycetes. Lac 1 showed the typica...
We identified a gene encoding a soluble quinoprotein glucose dehydrogenase homologue in the hyperthermophilic archaeon Pyrobaculum aerophilum. The gene was overexpressed in Escherichia coli, after which its product was purified and characterized. The enzyme was extremely thermostable, and the activity of the pyrroloquinoline quinone (PQQ)-bound hol...
We isolated cDNA clones for novel protein kinases by expression screening of a cDNA library from the basidiomycetous mushroom Coprinus cinereus. One of the isolated clones was found to encode a calmodulin (CaM)-binding protein consisting of 488 amino acid residues with a predicted molecular weight of 53,906, which we designated CoPK12. The amino ac...
Although multifunctional Ca(2+)/calmodulin-dependent protein kinases (CaM-kinases) are widely distributed in animal cells, the occurrence of CaM-kinases in the basidiomycetous mushroom has not previously been documented. When the extracts from various developmental stages from mycelia to the mature fruiting body of Coprinus cinereus were analyzed b...
We isolated and characterized the class IV chitin synthase gene (Pochs1) from the edible basidiomycetous mushroom, Pleurotus ostreatus. Real-time quantitative RT-PCR analysis of the transcriptional expression pattern of Pochs1 in the course of fruit-body formation of P. ostreatus revealed that the transcriptional level of Pochs1 is higher in the st...
We isolated and characterized the genomic and complementary DNAs encoding a chitin synthase from an edible basidiomycetous
mushroom, Lentinula edodes. The gene (which we designated Lechs1) contains a large open reading frame encoding a polypeptide of 1937 amino acid residues. The open reading frame is interrupted
by 14 small introns (49–116 bp). Th...
We isolated and characterized the genomic and complementary DNAs encoding a chitin synthase from an edible basidiomycetous mushroom, Lentinula edodes. The gene (which we designated Lechs1) contains a large open reading frame encoding a polypeptide of 1937 amino acid residues. The open reading frame is interrupted by 14 small introns (49–116 bp). Th...
We isolated and characterized the class IV chitin synthase gene (Pochs1) from the edible basidiomycetous mushroom, Pleurotus ostreatus. Real-time quantitative RT-PCR analysis of the transcriptional expression pattern of Pochs1 in the course of fruit-body formation of P. ostreatus revealed that the transcriptional level of Pochs1 is higher in the st...
Free d-alanine was detected in a cell extract of the fruit-body of an edible basidiomycetous mushroom, Lentinus edodes (Shiitake), by means of reverse-phase high performance liquid chromatography. We also found an amino acid racemase activity in L. edodes fruit-body, and purified the enzyme. The enzyme has a molecular weight of approximately 86,000...
The gene encoding the endo-beta-N-acetylglucosaminidase from Flavobacterium sp. (Endo-Fsp) was sequenced. The Endo-Fsp gene was overexpressed in Escherichia coli cells, and was purified from inclusion bodies after denaturation by 8 M urea. The renatured Endo-Fsp had the same optimum pH and substrate specificity as the native enzyme. Endo-Fsp had 60...
The gene encoding endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) was cloned, and its nucleotide sequence was determined. A single open reading frame consisting of 1935 base pairs and encoding a polypeptide composed of signal peptides of 24 amino acids and a mature protein of 621 amino acids was found. The primary structu...
To study the mechanism of regulation and structure/function relationship of the Pleurotus ostreatus manganese (11) peroxidase (MnP), we amplified the full-length genomic and complementary DNAs for the major isozyme of the MnP mainly by the cassette-primer PCR technique and then sequenced them. The cDNA contained an open reading frame of 1083 by enc...
The activity of aryl-alcohol oxidase was detected in the mycelial extracts of a lignin-degrading basidiomycete, Phanerochaete chrysosporium. The induction of production of the enzyme by aryl-alcohols was suggested. The enzyme was purified to homogeneity. The molecular weight was estimated to be about 78,000. The prosthetic group was found to be FAD...
Citations
... Hu et al. (2019) described the yellow strain of cultivated F. velutipes (FVY), which is known as Jinhua mushroom in Taiwan, and it has become popular among customers due to its distinct texture that is utterly different from the white strain of this species (FVW). Also, it is a commercially important cultivated edible mushroom especially in China and Japan (Yang et al. 2012;Dong et al. 2017;Cesur et al. 2022). But, in the 1980s, Magae et al. (2005) reported some cases with degenerate strains of F. velutipes, which exhibiting the loss of the fruiting bodies formation, which led to substantial financial losses in the mushroom industry (Cesur et al. 2022). ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/806972630781953-1569408676418_Q64/Aylin-Cesur.jpg)
... This might be because Lac2 was a single laccase or the redox potential of Lac2 was low. Some mushrooms contain multiple laccase-coding genes and can secrete a variety of laccase isozymes, such as P. ostreatus (Zhuo et al., 2018), F. velutipes (Wang et al., 2015), and Grifola frondosa (Nitheranont, Watanabe, & Asada, 2017). We have found that G. sinense strain Gs-1 secreted three laccase isozymes (data not shown), which may be one of the reasons why G. sinense efficiently degrades AFB 1 in corn. ...
... DSD is highly specific to D-Ser. D-Thr, D-allo-Thr, and D-2-amino-3,4-dihydroxybutanoic acid were identified to be poor substrates for DSD (Ito et al., 2007(Ito et al., , 2008(Ito et al., , 2017. Other proteinogenic Lamino acids and their corresponding enantiomers do not act as the substrates. ...
... [37]. Interestingly, a similarly neutral pH optimum characterizes aryl-alcohol oxidase [38,39], which among others is supposed to cooperate in the extracellular environment. Alcohol oxidase activity in wood-degrading fungi was proved to be inhibited by ions (Cu 2+ , Fe 2+ ) [20], whereas AOs from yeast and other ascomycetous fungi are also affected by various compounds: alcohols [40], DMSO [41], or formaldehyde [42], H2O2 [40]. ...
... Mukhopadhyay and Banerjee [95] found that 1 mM sodium salt decreased laccase activity in Trametes versicolor. Chairin et al. [96] recorded that sodium chloride has an increasing inhibitory effect with increasing its concentration on Trametes polyzona and T. versicolor laccase and a reversible effect of more than 50% at 20 mM NaCl. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/484699823972354-1492572851714_Q64/Thanunchanok-Chairin.jpg)
... Anthraquinone dye (Reactive Blue 4) confirmed decolorization of approximately 61% and decreased the COD with the aid of using twofold in 12 h due to laccase remedy. (Chairin et al., 2013).. (Table 3) The majority of yeast research has focused on biosorption. Yeast has numerous advantages over bacteria and filamentous fungi, including the ability to withstand unfavorable circumstances as well as rapid growth similar to that of bacteria and filamentous fungi (Van Wylick et al., 2021). ...
... Although the basic steps in mushroom development have been determined, morphogenesis is still mostly unknown. The developmental stages ascertained are namely: 16 Hyphae knot formation Formation of initial aggregates Differentiation of the primordium with distinct cap and stipe tissues ...
Reference: An Overview on Mushrooms
... Isolation of a class IV chitin synthase gene from the edible basidiomycete Pleurotus ostreatus was conducted in the study by Nishihara et al. [47]. ...
... To overcome this drawback, they form complexes with organic acids naturally secreted by the fungus, such as malonic or oxalic acid (Hofrichter 2002;Wong 2009). There are three mnp genes, namely mnp1, mnp2, and mnp3, that have been isolated, and their products were characterized from P. ostreatus (Asada et al. 1995;Giardina et al. 2000;. Besides, both MnP2 and MnP3 have been purified (Kamitsuji et al. 2004;Sarkar et al. 1997). ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/326804649857024-1454927708181_Q64/Toru-Nakayama.jpg)
... In the case of negative controls, there was no evidence of elimination. Similar findings indicate that Bisphenol A (0.015%) was incubated with 1.5 U/mL of Lac 1 from Grifola frondos is capable of degrading Bisphenol A 30% without the use of a mediator in 6 hrs (46) . In just seven days, T. versicolor, a white-rot fungus, was found to be the most effective of the species, being reduced by 93% total phenol content under optimal conditions (47) . ...
