Adam M. Pickrum's research while affiliated with CUNY Graduate Center and other places
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Publications (7)
Achromobacter xylosoxidans (Ax) is an opportunistic pathogen and causative agent of numerous infections particularly in immunocompromised individuals with increasing prevalence in cystic fibrosis (CF). To date, investigations have focused on the clinical epidemiology and genomic comparisons of Ax isolates, yet little is known about disease patholog...
Patients affected by CF develop chronic bacterial infections characterized by inflammatory exacerbations and tissue damage. Advancements in sequencing technologies have broadened the list of opportunistic pathogens colonizing the CF lung. A. xylosoxidans is increasingly recognized as an opportunistic pathogen in CF, yet our understanding of the bac...
Achromobacter xylosoxidans is increasingly recognized as a colonizer of cystic fibrosis (CF) patients but the role that A. xylosoxidans plays in pathology remains unknown. This knowledge gap is largely due to the lack of model systems available to study the toxic potential of this bacterium. Recently, a phospholipase A 2 (PLA 2 ) encoded by a major...
Transmembrane Ser/Thr kinases containing extracellular PASTA domains are ubiquitous among Actinobacteria and Firmicutes species and regulate critical processes, including antibiotic resistance, cell division, and cell envelope homeostasis. Previous studies of PASTA kinase fragments revealed autophosphorylation at multiple sites. However, the functi...
The human gastrointestinal tract (GIT) is inhabited by a dense microbial community of symbionts. Enterococci are one of the earliest members of this community and remain core members of the GIT microbiota throughout life. Enterococci have also recently emerged as opportunistic pathogens and major causes of nosocomial infections. Although recognized...
Bacterial phospholipases act as intracellular and extracellular enzymes promoting the destruction of phospholipid barriers and inflammation during infections. Identifying enzymes with a common mechanism of activation is an initial step in understanding structural and functional properties. These properties serve as critical information for the desi...
Significance
Structure–function aspects of dynamic, membrane-associated proteins are difficult to mechanistically define. In this study, computational and biophysical methods are applied to identify a ubiquitin-interaction domain essential for the activity of a bacterial cytotoxin, ExoU. Introduction of mutations that diminish or improve ubiquitin...
Citations
... They also possess secretion systems well-known for contributing to virulence in many pathogens (44)(45)(46). Pro-inflammatory power was demonstrated both in vitro and in vivo (47)(48)(49)(50) showing that Achromobacter spp. are capable of inducing inflammation in a similar way to P. aeruginosa (48,50). ...
... They also possess secretion systems well-known for contributing to virulence in many pathogens (44)(45)(46). Pro-inflammatory power was demonstrated both in vitro and in vivo (47)(48)(49)(50) showing that Achromobacter spp. are capable of inducing inflammation in a similar way to P. aeruginosa (48,50). ...
... All the above mentioned stages in the life cycle of pathogens are coordinated by Quorum-Sensing (QS) signaling [6]. Survival mechanisms help these bacteria to compete with each other and with other representatives of the lung microbiome, so microbial diversity in such infections becomes minimal [7]. ...
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... IreK responds to cephalosporin exposure by becoming more highly autophosphorylated, enhancing IreK activity to drive signaling through its downstream pathway leading to cephalosporin resistance (Labbe & Kristich, 2017). We identified three sites of phosphorylation on the activation loop of IreK (T163, T166, T168) as well as an additional site of phosphorylation (T218) on an alpha helix in the kinase helical C-lobe (distinct from the activation loop), that each influence IreK activity in vivo (Labbe et al., 2019). Functional studies revealed that phosphorylation of the IreK activation loop enhances kinase activity in vivo. ...
... gbuA encodes a component of the choline ABC transport system with applications in osmoregulation 34,35 (Fig. 2b). Three genes associated with adhesion-related functions were identified in the pangenome of Lc. raffinolactis including cell-surface-related proteins, such as elongation factor Tu (tuf), and sortase A-dependent enzymes (StrA) (Fig. 2b), which is known to affect the mucin binding ability of microorganisms 36 [37][38][39] . Supportive annotations for the production of the metabolites lactate and acetate and a number of genes linked to GABA production were widely found across the Lc. ...
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... Furthermore, a patatin-like phospholipase is involved in axon functionality of neurons 75 . Patatin-like phospholipases are also found in pathogenic bacterial species and act as toxins in host-pathogen interactions 53,76 . One of the best characterized patatin-like phosphlipases is ExoU, a cytotoxic effector protein of P. aeruginosa secreted through the type III secretion system upon host cell contact 77 . ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/282190173753350-1444290788394_Q64/David-Anderson-4.jpg)
... Although many of the foundational SDSL studies were directed toward understanding the structure and function of the integral membrane G-protein coupled receptor (GPCR) rhodopsin [6], the methods developed in those studies have proven adaptable to a wide variety of systems including soluble and peripheral membrane proteins. We have used SDSL EPR to examine the structure and stability of the bacterial outer membrane β-barrel protein FepA [7][8][9][10], to examine the membrane interactions of synthetic antimicrobial peptides [11][12][13], and to investigate the bacterial phospholipase, ExoU [14][15][16][17][18][19]. Our ongoing studies of ExoU are illustrative of the power of SDSL EPR in probing the structure and dynamics of a peripheral membrane protein. ...
