October 2017
·
73 Reads
·
5 Citations
[This corrects the article DOI: 10.1371/journal.pone.0150763.].
What is this page?
This page lists the scientific contributions of an author, who either does not have a ResearchGate profile, or has not yet added these contributions to their profile.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
Publications (52)
January 2017
·
54 Reads
ORYZA- An International Journal on Rice
October 2016
·
642 Reads
·
23 Citations
Planta
Main conclusion Genetically engineered rice lines with broad insecticidal properties against major lepidopteran pests were generated using a synthetic, truncated form of vegetative insecticidal protein (Syn vip3BR) from Bacillus thuringiensis. The selectable marker gene and the redundant transgene(s) were eliminated through Cre/ lox mediated recombination and genetic segregation to make consumer friendly Bt -rice. For sustainable resistance against lepidopteran insect pests, chloroplast targeted synthetic version of bioactive core component of a vegetative insecticidal protein (Syn vip3BR) of Bacillus thuringiensis was expressed in rice under the control of green-tissue specific ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene promoter. The transgenic plants (in Oryza sativa indica Swarna cultivar) showed high insect mortality rate in vitro against major rice pests, yellow stem borer (Scirpophaga incertulas), rice leaf folder (Cnaphalocrocis medinalis) and rice horn caterpillar (Melanitis leda ismene) in T1 generation, indicating insecticidal potency of Syn vip3BR. Under field conditions, the T1 plants showed considerable resistance against leaf folders and stem borers. The expression cassette (vip-lox-hpt-lox) as well as another vector with chimeric cre recombinase gene under constitutive rice ubiquitin1 gene promoter was designed for the elimination of selectable marker hygromycin phosphotransferase (hptII) gene. Crossing experiments were performed between T1 plants with single insertion site of vip-lox-hpt-lox T-DNA and one T1 plant with moderate expression of cre recombinase with linked bialaphos resistance (syn bar) gene. Marker gene excision was achieved in hybrids with up to 41.18 % recombination efficiency. Insect resistant transgenic lines, devoid of selectable marker and redundant transgene(s) (hptII + cre-syn bar), were established in subsequent generation through genetic segregation.
April 2016
·
116 Reads
·
7 Citations
Journal of Plant Biochemistry and Biotechnology
Identification and characterization of plant promoters from wild rice genotypes showing inducible expression under soil water stress (SWS) is desirable for transgene expression to generate stress tolerant rice cultivars. A comparative expression profiling of Wsi18, a group 3 LEA gene, revealed differential response under SWS conditions between modern cultivated rice (IR20) and its wild progenitor (Oryza nivara). Wsi18 promoter from O. nivara showed enhanced inducible expression of the reporter gusA gene, encoding β-glucuronidase, in transgenic rice plants in comparison to similar promoter from IR20. Deletion analysis unravelled the cis-acting regulatory elements minimally required for optimal expression of Wsi18 promoter from O. nivara under SWS condition. This is the first report of characterization of an inducible promoter from a wild rice genotype to drive the gene expression under water stress conditions. The Wsi18 promoter element from the wild rice genotype can be used in future genetic manipulation strategies for the generation of SWS tolerant rice cultivars with improved yield characteristics.
March 2016
·
10 Reads
Schematic representation of OsbZIP23 promoter-GFP reporter gene construct in pCAMBIA1300 plasmid. Promoter sequence of 1612 bp from drought tolerant wild genotype O. rufipogon was fused to GFP reporter gene and the transgenic lines developed with this genetic construct were designated as RuP series. (TIF)
March 2016
·
241 Reads
·
39 Citations
Drought is one of the major limiting factors for productivity of crops including rice (Oryza sativa L.). Understanding the role of allelic variations of key regulatory genes involved in stress-tolerance is essential for developing an effective strategy to combat drought. The bZIP transcription factors play a crucial role in abiotic-stress adaptation in plants via abscisic acid (ABA) signaling pathway. The present study aimed to search for allelic polymorphism in the OsbZIP23 gene across selected drought-tolerant and drought-sensitive rice genotypes, and to characterize the new allele through overexpression (OE) and gene-silencing (RNAi). Analyses of the coding DNA sequence (CDS) of the cloned OsbZIP23 gene revealed single nucleotide polymorphism at four places and a 15-nucleotide deletion at one place. The single-copy OsbZIP23 gene is expressed at relatively higher level in leaf tissues of drought-tolerant genotypes, and its abundance is more in reproductive stage. Cloning and sequence analyses of the OsbZIP23-promoter from drought-tolerant O. rufipogon and drought-sensitive IR20 cultivar showed variation in the number of stress-responsive cis-elements and a 35-nucleotide deletion at 5'-UTR in IR20. Analysis of the GFP reporter gene function revealed that the promoter activity of O. rufipogon is comparatively higher than that of IR20. The overexpression of any of the two polymorphic forms (1083 bp and 1068 bp CDS) of OsbZIP23 improved drought tolerance and yield-related traits significantly by retaining higher content of cellular water, soluble sugar and proline; and exhibited decrease in membrane lipid peroxidation in comparison to RNAi lines and non-transgenic plants. The OE lines showed higher expression of target genes-OsRab16B, OsRab21 and OsLEA3-1 and increased ABA sensitivity; indicating that OsbZIP23 is a positive transcriptional-regulator of the ABA-signaling pathway. Taken together, the present study concludes that the enhanced gene expression rather than natural polymorphism in coding sequence of OsbZIP23 is accountable for improved drought tolerance and yield performance in rice genotypes.
March 2016
·
8 Reads
Schematic representation of the overexpression (OE) construct of 1068 bp CDS of OsbZIP23 from O. rufipogon in pCAMBIA1300 plasmid. The transgenic rice lines developed with this genetic construct were designated as OER series. (TIF)
March 2016
·
13 Reads
Schematic representation of the overexpression (OE) construct of 1083 bp CDS of OsbZIP23 from O. nivara in pCAMBIA1300 plasmid. The transgenic rice lines developed with this genetic construct were designated as OER series. (TIF)
March 2016
·
3 Reads
Structural domain prediction of OsbZIP23 protein using ScanProsite web tool (http://prosite.expasy.org/scanprosite). In both drought tolerant genotypes (A) O. rufipogon and (B) O. nivara, three conserved transactivation domains (domain I, II and III) and one basic leucine zipper (bZIP) domain was predicted. Presence of three protein kinase C phosphorylation site (red pointed serine and threonine), five casein kinase II phosphorylation site (underlined threonine residues) and 11 N-myristoylation sites (indicated in blue colour) were also predicted. The 5-amino acid (AVEHA) deletion in O. rufipogon is indicated with gap. Note that this 5-amino acid sequence is AAEHA in all other genotypes tested except O. nivara and sorghum, which have AVEHA (Fig 1B). (TIF)
March 2016
·
7 Reads
List of primers used in this study. (DOCX)
Citations (35)
... Arabidopsis bZIP1 regulates ABA signal transduction by binding to the ABREs and changes the expressions of the ABA-responsive genes to tolerate cold stress (Sun et al. 2011). Functional study of OsZIP23 and OsZIP46 revealed their involvement in drought and salt resistance by directly regulating the expression of stressrelated genes through the ABA pathway (Dey et al. 2017;Tang et al. 2012;Xiang et al. 2008). The overexpression of the ABA-depended grapevine gene, VvABF2 gene could enhance osmotic stress tolerance in Arabidopsis which could reduce the cell membrane damage (Liu et al. 2019). ...
- Citing Article
- Full-text available
October 2017
... To relieve public concerns about GM crops, several genetic engineering techniques have been developed to manipulate transgene expression in confined tissues [15][16][17][18][19][20][21][22]. For instance, the Cre/loxP site-specific recombination system is the most-mentioned tool for precisely manipulating DNA introduced into transgenic plants due to its simplicity and efficiency [23,24]. In the system, Cre recombinase, responsible for specific excision of the sequences embedded by directly repeated loxP sites, has been applied to remove transgenes from the host genome [25]. ...
- Citing Article
- Full-text available
October 2016
Planta
... The WSI18 has been reported to be induced under drought stress in various studies (Joshee et al., 1998;Kaur et al., 2017;Langille et al., 2018;Yi et al., 2011). The upregulation of WSI18 expression (upregulated 6 fold and 14 fold in seedling and mature stage) in Keketi Joha further supports its potential role in response to water scarcity ( Figure 2 & Table S2). ...
- Citing Article
April 2016
Journal of Plant Biochemistry and Biotechnology
... Furthermore, OsPP2C09 exhibits PP2C phosphatase activity, and its mutation inhibits growth, including plant height and panicle length [27]. OsbZIP23 is a transcriptional regulator, and its overexpression shows a significant improvement in yield-related traits [28,53]. OsSIPP2C1 is negatively regulated by ABL1 and is responsible for panicle development in rice [29]. ...
- Citing Article
- Full-text available
March 2016
... However, a common problem associated with this control strategy is the development of insect resistance to the Bt toxin present in the transgenic plants [2,3]. Several approaches have been used to reduce or prevent the development of insect resistance including the use of refuge crops (providing sufficiently high populations of susceptible insects to prevent resistance genes from becoming homozygous), high expression of Cry genes in plants, deploying different Cry genes in individual plants in a crop (seed mixtures), and combining multiple Cry genes (i.e., stacking) in the same plant [4][5][6][7][8]. Of these, high expression and stacking of Cry genes in the same plant are considered the most practical effective strategies [1,5,9,10]. ...
- Citing Article
- Publisher preview available
February 2016
World Journal of Microbiology and Biotechnology
... Sesame powder has one of the highest oil contents among the main oilseeds [29]. It is also rich in proteins, carbohydrates, vitamins, polyunsaturated fatty acids in the composition, such as triacylglycerols fat, lignans (sesamin and sesamolin), tocopherol, phytosterols, phytates and other micronutrients [30]. Features of its chemical composition are reflected in the IR absorption spectra (Fig. 5, sample 4). ...
- Citing Article
September 2014
Journal of Crop Science and Biotechnology
... Plant plastidial ω-3 FAD7 catalyses the synthesis of C16 and C18 trienoic fatty acids, essential for cell membrane fluidity modulated cold temperature tolerance [69]. Silencing of the gene encoding chloroplastic ω-3 FAD7 (which synthesizes trienoic fatty acids essential for cold temperature tolerance) resulted in decreased levels of trienoic fatty acids and enhanced high-temperature acclimation in FAD7-silenced transgenic tobacco plants [70]. ...
- Citing Article
- Publisher preview available
January 2016
Plant Cell Reports
... Two sesame cytochrome P450 genes responsible for sesamin and sesamolin were targeted using A. rhizogenes-mediated transformation, showing the feasibility of CRISPR-based genome editing in sesame (You et al. 2022). The particle bombardment in cultivar Rama, the genetic transformation used in apical meristematic tissue, showed approximately 16% transformation efficiency (Bhattacharyya et al. 2015), which is almost double the Agrobacterium-mediated transformation efficiency in the same cultivar (Gayatri and Basu 2020). The enhanced transformation efficiency could be due to the reduced toxicity or browning of the tissue caused by Agrobacterium; however, the particle bombardment method needs to be evaluated for successful transformation on other sesame varieties. ...
- Citing Article
August 2015
Plant Cell Tissue and Organ Culture
... In light of the atomic structure of Vip3 proteins, it has become evident that Domains IV and V do not have a critical role in the core structure of the protein, and because of this it is likely that they did not have strong restrictions of accumulating changes during the evolutionary diversification of Vip3 proteins. Nevertheless, there are many reports showing a drastic loss of toxicity as a result of point mutations and truncations in Domains IV and V leading to protein instability (Banyuls et al., 2018;Gayen et al., 2012;Gayen et al., 2015;Li et al., 2007;Quan and Ferré, 2019;Selvapandiyan et al., 2001). Also, some point mutations in Domains IV and V were recently reported to increase the toxicity of Vip3Aa in some species . ...
- Citing Article
- Full-text available
April 2015
Planta
... SWAP70 functions as a Rac/Rop guanine nucleotide-exchange factor in rice and regulates immune responses through activation of important signaling factors like Rho GTPase OsRac1.OsFAD7 encoding fatty acid desaturase7 was identified as imparting disease resistance against blast by suppressing of ω-3 Fatty Acid desaturases which contribute to signaling on defense responses (Yara et al. 2007). Interestingly this region chr3:6,165,992…29,813,274 was also harbouring several QTLs and genes for grain size related traits like GS3, qGW3, qGY3, qGL3, qLWR3, GL3.1 and genes like pdhk encoding pyruvate dehydrogenase kinase (Mao et al. 2003;Miyata et al. 2007;Wan et al. 2005;Mukherjee et al. 2012). Gene bph19(t) is also located in this region (Chen et al. 2006a, b). ...
- Citing Article
- Publisher preview available
July 2012