Content uploaded by L. O. Sanni
Author content
All content in this area was uploaded by L. O. Sanni on Jul 23, 2015
Content may be subject to copyright.
39
NIGERIAN FOOD JOURNAL, VOL. 25, No. 2, 2007 (www.ajol.info/journals/nifoj) ISSN 0189-7241
Adeniji, T.A.1*, Sanni, L.O.2, Barimalaa, I.S.1, and Hart, A.D.1
Department of Food Science and Technology,
Rivers State University of Science and Technology,
P.M.B. 5080, Nkpolu, Port Harcourt, Nigeria.
2International Institute of Tropical Agriculture, High Rainfall Station,
Onne, P.M.B. 008, Nchia-Eleme, Port Harcourt, Nigeria.
*Address for Correspondence, E-mail: tiradeniji@yahoo.com
Mineral composition of five improved varieties of cassava
ABSTRACT
Five new cassava mosaic disease resistant (CMD) varieties
released to farmers in Nigeria were evaluated for mineral
composition. Results show that TME 419 differed
significantly (p<0.05) from other varieties in calcium (900ìg/
g) and manganese (32.3ìg/g) content. The hybrid check
(TMS 30572) and TMS 98/0505 were significantly different
(p<0.05) from other varieties in magnesium (1300ìg/g) and
potassium (3900ìg/g), respectively. TMS 97/2205 differed
significantly (p<0.05) from other varieties in phosphorus
(1200ìg/g) and sodium (63.7ìg/g). Data also revealed that
TMS 98/0581 was significantly different (p<0.05) from
other varieties in iron (184.2ìg/g) while TMS 98/0510
differed significantly (p<0.05) from other varieties both in
copper (10.9ìg/g) and zinc (8.5ìg/g). Chipping and grating
had no significant effect (p>0.05) on calcium, sodium,
copper, and zinc content of cassava flour. However,
treatment effect was obvious in other minerals, chipped
having a significant difference (p<0.05) in magnesium,
potassium and manganese contents compared to grated
samples. Conversely, grated samples differed significantly
(p<0.05) in phosphorus and iron compared to chipped
samples. Estimated daily recommended dietary allowances
(RDA) from major minerals found in CMD varieties show
that calcium, potassium, phosphorus and copper
contributions to RDA ranged from 17.5-22.5%, 13.8-20.7%,
32.1-42.9%, and 127.8-302.8% in both adult males and
females, respectively if 250g flour is eaten daily. The
highest level of magnesium contribution (81.3% and
104.8%) to RDA was found in TMS 30572 for adult males
and females, respectively, from 250 g flour. TMS 98/0581
was highest in iron contribution to RDA with 575.6% and
255.8% for adult males and females, respectively, provided
250 g flour is eaten. TMS 98/0510 had the highest level of
contribution to RDA, 19.3% for adult males, while 26.6%
is accruable to adult female, provided 250 g flour is
consumed. New cassava varieties may constitute
important raw materials in the food industries based on
their nutritional composition.
Key words: Minerals, nutrition, recommended daily
allowance, raw materials, food industry.
INTRODUCTION
Cassava (Manihot esculenta Crantz) is a major
food crop in the humid and sub-humid parts of
Africa and a major source of energy for millions of
people in these regions (Asiedu, et. al. 1992). It is
the most important root crop in Nigeria in terms of
food security, employment creation, and income
generation for crop producing households (Ugwu
and Ukpabi, 2002). World production of cassava
was 160 million tons of fresh roots, with 80 million
tons produced in Africa, out of which 34 million tons
is produced in Nigeria (FAO, 1994) and by this
figure, Nigeria is the largest producer of cassava in
the world. The daily per capital consumption of
cassava in Nigeria contributed about one megajoule
Full ReseachArticle
40 NIGERIAN FOOD JOURNAL, VOL. 25, No. 2, 2007 (www.ajol.info/journals/nifoj) ISSN 0189-7241
(mJ) to the diet. Estimated household consumption
is about 30 million metric tons, with a marketable
surplus for industrial demand of about 10% of total
production (Nwachukwu, 2005). The adaptation
of cassava to the food and farming systems and
multiplicity of uses makes it indispensable to food
security. Cassava is a cheap source of carbohydrate
from nutritional point of view. The roots of currently
available varieties are relatively low in protein,
although in Central Africa, substantial amounts of
protein are derived from cassava leave, which are a
popular vegetable (Berry, 1993).
The major uses of cassava in Nigeria (Philip, et
al., 2005) include flour, which involves several unit
operations, resulting in flour of different properties,
which are used for various purposes. Other products
include gari, creamy-white, granular flour with a
slightly fermented flavour and slightly sour taste.
Fresh roots may also be boiled and pounded to
obtain pounded fufu, which is popular in Ghana,
and to some extent in Nigeria and Cameroon (Hahn,
1997). Fresh cassava roots also finds considerable
use as a feed for livestock such as goat, sheep, cattle,
and particularly pigs (Ihekoronye and Ngoddy,
1985). Nwosu (2005) identified some of the
industrial uses of cassava including ethanol
production, livestock feeds, confectioneries,
monosodium glutamate processing, sweeteners,
glues, textiles and pharmaceuticals.
The International Institute of Tropical
Agriculture (IITA) had developed several varieties
of cassava mosaic disease resistant cultivars. New
varieties are being currently disseminated in Nigeria
and many parts of West and Central Africa (WCA)
and East and Southern Africa (ESA) to improve
agricultural productivity, rural income and generate
raw materials for local industries. The mineral
composition of five top varieties of CMD released
cultivars was therefore investigated to enhance
delivery, utilization and industrial potential.
MATERIALS AND METHODS
Five new released varieties of cassava (98/
0505, TME 419, 97/2205, 98/0581, and 98/0510,
including the reference, TMS 30572) were
investigated. Tuberous cassava roots were harvested
one year after planting from the experimental station
of the International Institute of Tropical Agriculture,
High Rainfall Station, Onne agro ecology, located
on Latitude 04o 43' N, Longitude 07o 01' E and 10m
Altitude, near Port Harcourt, Nigeria. Root samples
were peeled manually with the aid of stainless steel
kitchen knife. Some aliquots were chipped into small
pieces, while some were grated prior to dehydration.
Samples were placed in petri dish and covered with
filter paper to avoid contamination, and transferred
carefully into Forced-Air Sanyo Gallenkamp
Moisture Extraction Oven and dried at 65oC for
about 48 hours and milled with the aid of stainless
Kenwood Chef Warring Blender, Model KM001
series. Mineral analysis was performed using the
procedure described by Allen et al., (1984). Daily
recommended dietary allowances (RDA) from major
minerals found in CMD varieties were estimated with
reference to Institute of Medicine, National
Academy of Sciences (2002) tables of RDA. All
mineral elements investigated were determined
spectrophotometrically on the Buck Scientific
Atomic Absorption/Emission Spectrophotometer
205 in the Plant Anatomy and Physiology Research
Laboratory, Faculty of Science, University of Port
Harcourt, Herbarium, Port Harcourt, Nigeria.
Data Analysis
The data generated were analysed using
Statistical Analysis Systems version 9.1 SAS (2003)
software package. Significance of treatment means
was tested at 5% probability level using Duncan’s
New Multiple Range Test (DNMRT).
RESULTS AND DISCUSSION
Mineral profile of CMD varieties is presented
in Table 1, which shows a significant difference
(p<0.05) between TME 419 and other varieties in
Determination of Some Nutritional Valuable Minerals in cassava....................................................................Adeniji et al
41
NIGERIAN FOOD JOURNAL, VOL. 25, No. 2, 2007 (www.ajol.info/journals/nifoj) ISSN 0189-7241
calcium (900ìg/g) and manganese (32.3ìg/g) content.
The range of calcium (700-900µg/g) levels found in
new CMD varieties is higher than 330µg/g earlier
reported in cassava root (Ihekoronye and Ngoddy,
1985). The physiological roles of minerals in human
diet have been widely reported (Ihekoronye and
Ngoddy, 1985; Okaka et al., 1992; Onigbinde,
2001). The hybrid check, TMS 30572 and TMS
98/0505 were significantly different (p<0.05) from
other varieties in magnesium (1300ìg/g) and
potassium (3900ìg/g), respectively. TMS 97/2205
differed significantly (p<0.05) from all other varieties
in phosphorus (1200ìg/g) and sodium (63.7ìg/g)
content. TMS 98/0581 was significantly different
(p<0.05) from other varieties in iron (184.2ìg/g) while
TMS 98/0510 differed significantly (p<0.05) from
other varieties both in copper (10.9ìg/g) and zinc
(8.5ìg/g). The levels of iron found in new CMD
varieties were much higher than 7µg/g reported in
fresh cassava roots (FAO, 1998) and 64µg/g
reported in sweet potato (Ihekoronye and Ngoddy,
1985). Moreover, this new data surpassed 1.06mg/
100g of iron obtained in raw green plantain
(Ahenkora, et. al., 1996) and 0.2-0.3mg/100g
values obtained in flours of two sweet potato varieties
(Onuh, et al., 2004). The same trend was observed
for zinc, when compared to a meager level of 0.26mg/
100g obtained by Ahenkora et al. (1996) in Apantu
pa plantain in Ghana. Chipping and grating had no
significant effect (p>0.05) on calcium, sodium,
copper, and zinc content of cassava flour (Table 3).
Chipped samples had a significant difference
(p<0.05) in magnesium, potassium and manganese
contents compared to grated samples. Conversely,
grated samples differed significantly (p<0.05) in
phosphorus and iron compared to chipped samples.
Ebuehi (2005) reported significant losses in various
minerals including calcium, magnesium, phosphorus,
iron, sodium and chloride ions identified in the roots
and raw leaves of cassava as a result of boiling.
Boiling and frying has also been implicated in losses
of certain micronutrient in plantain including iron,
copper and zinc (Ahenkora, et al., 1996). Daily
recommended dietary allowances from major
minerals found in CMD varieties were estimated
(Table 3). Results show that calcium, potassium,
phosphorus and copper contribution to RDA ranged
from 17.5-22.5%, 13.8-20.7%, 32.1-42.9%, and
127.8-302.8% in both adult males and females,
respectively if 250g flour is eaten daily. The highest
level of magnesium contribution (81.3% and 104.8%)
to RDA was found in TMS 30572 for adult males
and females, respectively, from 250 g flour. TMS
98/0581 was highest in iron contribution to RDA
with 575.6% and 255.8% for adult males and
females, respectively. The trend with zinc follows a
different pattern, with TMS 98/0510 having the
highest level of contribution to RDA, 19.3% for adult
males, while 26.6% is accruable to adult female,
provided that 250 g flour is consumed. Increased
production and utilization of new cassava varieties
may contribute to offset micronutrient deficiencies
prevailing in Africa.
Determination of Some Nutritional Valuable Minerals in cassava....................................................................Adeniji et al
42 NIGERIAN FOOD JOURNAL, VOL. 25, No. 2, 2007 (www.ajol.info/journals/nifoj) ISSN 0189-7241
Table 1. Mineral composition of cassava mosaic disease resistant (CMD) varieties
Cultivar Ca Mg K P Na Mn Fe Cu Zn_
µg/g
TMS 97/2205 800b800c2900e1200a63.7a28.1d163.5d6.3c8.0c
TMS 98/0505 700c700d3900a1000b50.3e28.2c184.0b4.6d6.0e
TMS 98/0510 700c900b3500b1000b46.9f24.5e117.3f10.9a8.5a
TMS 98/0581 800b700d3100c1200a50.4d28.2c184.2a6.3c5.6f
TME 419 900a700d2600f1000b58.5b32.3a168.5c8.1b7.6d
TMS 30572 800b1300a3000d900c55.1c31.9b123.1e6.3c8.3b
Values in the same column with different letters are significantly different at p<0.05.
Table 2. Effect of processing on mineral composition of cassava mosaic disease resistant varieties
Ca Mg K P Na Mn Cu Fe Zn
ug/g
Treatment
Chipped 800a1040a 3920a720b53.53a30.80a7.28a105.64b6.44a
Grated 783a 850b 3170b1050a54.15a28.87b7.08a156.77a7.33a
Chipped=Fresh cassava roots were chipped after peeling prior to dehydration, Grated=Fresh cassava roots were
grated after peeling prior to dehydration.
Values in the same column with different letters are significantly different at p<0.05.
Determination of Some Nutritional Valuable Minerals in cassava....................................................................Adeniji et al
43
NIGERIAN FOOD JOURNAL, VOL. 25, No. 2, 2007 (www.ajol.info/journals/nifoj) ISSN 0189-7241
Table 3. Mineral composition of cassava mosaic disease resistant varieties to daily recommended dietary
allowance
% Minerals from daily consumption of 250g flour
Adult Male/Female
Cultivar Ca Mg K P Cu Fe Zn
TMS 97/2205 20/20 50/64.5 15.4/15.4 42.9/42.9 175/175 510.9/227.1 18.2/25
TMS 98/0505 17.5/17.5 43.8/56.5 20.7/20.7 35.7/35.7 127.8/127.8 575/255.6 13.6/18.8
TMS 98/0510 17.5/17.5 56.3/72.6 18.6/18.6 35.7/35.7 302.8/302.8 366.6/162.9 19.3/26.6
TMS 98/0581 20/20 43.8/56.5 16.5/16.5 42.9/42.9 175/175 575.6/255.8 12.7/17.5
TME 419 22.5/22.5 43.8/56.5 13.8/13.8 35.7/35.7 225/225 526.6/234.0 17.3/23.8
TMS 30572 20/20 81.3/104.8 15.9/15.9 32.1/32.1 175/175 384.7/170.9 18.9/25.9
RDA for adult males and females are 1000mg Ca each, 400mg and 310mg Mg, respectively, 4.7g K
each, 700mg P each, 900ìg/g Cu each, 8mg and 18mg Fe, respectively and 11mg and 8mg Zn
(Institute of Medicine, National Academy of Sciences, 2002).
Estimated values were obtained from data on mineral composition of CMD varieties in Table 1.
REFERENCES
Ahenkora, K., Kyei, M.A., Marfo, E.K, Banful, B.
(1996). Nutritional composition of False Horn
Apantu pa plantain during ripening and processing.
African Crop Science Journal, 4 (2): 243-247.
Allen, S.E., Grimshaw, H.M, Parkinson, J.A. and
Quarmby, C. (1984). Chemical analysis of ecological
materials. Edited by S.E. Allen. Blackwell Scientific
Publications, Oxford London, Edinburgh,
Melbourne, 565pp.
Asiedu, R., Ng, S.Y.C., Vuylsteke, D., Terauchi,
R., and Hahn, S.K. (1992). Analysis of the need
for biotechnology research on cassava, yam, and
plantain. In: Thottappilly, G., Monti, L., Mohan Raj,
D.R., and Moore, A.W. (eds.). Biotechnology:
Enhancing research on tropical crops in Africa. CTA/
IITA co-publication. IITA, Ibadan, Nigeria, 376pp.
Berry, S.S. (1993). Socio-economic aspects of
cassava cultivation and use in Africa: implication for
the development of appropriate technology. COSCA
Working Paper No. 8, Collaborative Study on
Cassava in Africa, International Institute of Tropical
Agriculture, Ibadan.
Ebuehi, O.A.T., Babalola O and Ahmed, Z (2005).
Phytochemical, nutritive and anti-nutritive
composition of cassava (Manihot esculenta L)
tubers and leaves. Nigerian Food Journal, 23: 40-
46.
FAO (1994). FAO Production Year Book, Food
and Agricultural Organisation of the United Nations,
Rome.
FAO (1998). The Food Composition Tables for
use in Africa. FAO and US Department of Health
and Welfare.
Determination of Some Nutritional Valuable Minerals in cassava....................................................................Adeniji et al
44 NIGERIAN FOOD JOURNAL, VOL. 25, No. 2, 2007 (www.ajol.info/journals/nifoj) ISSN 0189-7241
Hahn, S.K. (1997). Traditional processing and
utilization of cassava in Africa. IITA Research Guide
41. Training Program, International Institute of
Tropical Agriculture (IITA), Ibadan, Nigeria, Fourth
Edition, p 42.
Ihekoronye, A.I. and Ngoddy, P.O. (1985).
Integrated Food Science and Technology for the
Tropics. Macmillan Publishers Ltd., London and
Basingstoke, p 386.
Institute of Medicine, National Academy of Sciences
(2002). Dietary Reference Intakes for Energy,
Carbohydrate, Fiber, Fat, Fatty Acids, Cholesterol,
Protein, and Amino Acids, Food and Nutrition
Board, Washington D.C., National Academic Press.
Nwachukwu, E.O. (2005). Cassava Processing and
Equipment Assessment: Tool for Poverty Alleviation.
Presented at State Workshop on Empowering the
Grassroots Through Promotion of Commercial
Cassava Production and Processing, October 11-
12, Owerri, Nigeria.
Nwosu, O.S.U. (2005). Cassava Cultivation,
Varieties Available and Land Preparation. Presented
at State Workshop on Empowering the Grassroots
Through Promotion of Commercial Cassava
Production and Processing, October 11-12, Owerri,
Nigeria.
Okaka, J.C., Akobundu, E.N.T., and Okaka,
A.N.C. (1992). Human Nutrition: an integrated
approach, Enugu State University of Science and
Technology, Enugu, Nigeria.
Onigbinde, A.O. (2001). Human Nutrition
(Biochemical Integration). Ilupeju Publishers Ltd.,
Benin City, Nigeria, p300.
Onuh, J.O., Akpapunam, M.A., and Iwe, M.O.
(2004). Comparative studies of the physico-
chemical properties of two local varieties of sweet
potato flours. Nigerian Food Journal, 2: 141-146.
Philip, T., D. Taylor, L. Sanni, R. Okechukwu, C.
Ezedinma, M. Akoroda, J. Lemchi, P. Ilona, F.
Ogbe, E. Okoro and A.G.O. Dixon (2005). The
Nigerian cassava industry: Statistical handbook,
International Institute of Tropical Agriculture (IITA),
Ibadan, p 94.
SAS (2003). Statistical Analysis Software (SAS)
Systems for Windows, SAS Institute Inc., Cary, NC,
USA.
Ugwu, B.O. and Ukpabi, U.J. (2002). Potential of
soy-cassava flour processing to sustain increasing
cassava production in Nigeria. Outlook on
Agriculture, Vol. 31, No. 2, p. 129-133.
Determination of Some Nutritional Valuable Minerals in cassava....................................................................Adeniji et al