In 1986, when Tom Levy indicated that distinct regional cultures emerged in the Late Chalcolithic period, he did not refer to the Galilee, as available information on that region was sparse. In the same year, Frankel and Gophna defined Galilean painted “Abu-Sinan” pottery, but additional information regarding the Late Chalcolithic material culture in the mountainous Galilee was still scarce and mainly based on a few survey collections. The discovery of the Peqi‘in secondary-burial cave in 1995 changed the hitherto general impression that the Galilee during the second half of the fifth millennium BCE was a barely populated periphery. Since then, interest in the mountainous Galilee during this period has increased, engendering additional surveys and some excavations. The updated information shows that this area housed more than 30 settlements, and that its material culture was in general typical of the period and the Ghassulian culture. However, variations in pottery types, decoration, and motifs as well as flint, differentiates this area. We suggest that these distinctive Galilean characteristics should be considered a Ghassulian Galilean sub-culture.