The complete mitochondrial genome sequence of Rana dabieshanensis (Anura: Ranidae)

Abstract

Rana dabieshanensis is a species of frog within the family Ranidae. In this study, we assembled a complete mitochondrial genome (mito-genome) for R. dabieshanensis by high-throughput sequencing technology. It is 18,291 bp and includes 13 protein-coding genes, 22 tRNA genes, two rRNA genes and one control region. The nucleotide composition is A: 27.0%, T: 28.6%, C: 29.3% and G: 15.2%. Two overlaps among the 13 protein-coding genes were found: ATP8/ATP6, ND4L/ND4. The study of phylogenetic analysis based on complete mitochondrial genome showed that there was close genetic relationship between R. dabieshanensis and R. omeimontis and it is useful for systematic analyses of genus Rana.

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MITOGENOME ANNOUNCEMENT
The complete mitochondrial genome sequence of Rana dabieshanensis
(Anura: Ranidae)
Jingmei Gan
a
, Ying Li
a
, Yaqiong Wan
b
, Jiaqi Li
b
and Baowei Zhang
a
a
School of Life Sciences, Anhui University, Hefei, China;
b
Environmental Protection Key Laboratory on Biosafety, Nanjing Institute of
Environmental Science, Ministry of Ecology and Environment, Nanjing, Jiangsu, China
ABSTRACT
Rana dabieshanensis is a species of frog within the family Ranidae. In this study, we assembled a com-
plete mitochondrial genome (mito-genome) for R. dabieshanensis by high-throughput sequencing tech-
nology. It is 18,291bp and includes 13 protein-coding genes, 22 tRNA genes, two rRNA genes and one
control region. The nucleotide composition is A: 27.0%, T: 28.6%, C: 29.3% and G: 15.2%. Two overlaps
among the 13 protein-coding genes were found: ATP8/ATP6,ND4L/ND4. The study of phylogenetic ana-
lysis based on complete mitochondrial genome showed that there was close genetic relationship
between R. dabieshanensis and R. omeimontis and it is useful for systematic analyses of genus Rana.
ARTICLE HISTORY
Received 5 February 2021
Accepted 17 April 2021
KEYWORDS
Rana dabieshanensis;
mitochondrial genome;
phylogenetic analysis
The Dabie mountain brown frog (Rana dabieshanensis)
belongs to the family Ranidae, which is distributed in Dabie
Mountains regions, Anhui Province, China (Wang et al. 2017).
It is controversy about the classification of species within the
genus Rana in the Dabie Mountains (Wang 2018). To better
understand the mitochondrial genomic characteristics, phyl-
ogeny and evolution of the R. dabieshanensis, we determined
and described the mitogenome sequence of R. dabieshanen-
sis in order to obtain basic genetic information about
this species.
A specimen of R. dabieshanensis was collected from
Sucheng County, Anhui Province, China (3131013.6100N,
11632044.8700E) and stored in Anhui University Museum,
Research Center for Biology (Voucher number: DBS202001).
The genomic DNA extraction, library preparation and Illumina
sequencing were done by Novogene Bioinformatics
Technology Co. Ltd. (Tianjin, China).
We obtained the complete mitochondrial genome of R.
dabieshanensis is 18,291 bp and submitted to the GenBank
with the accession number MW526989. It included 13 pro-
tein-coding genes, two ribosomal RNAs genes, 22 transfer
RNAs genes and one control region. The overall nucleotide
composition is A: 27.0%, T: 28.6%, C: 29.3% and G: 15.2%,
with a total A þT content of 55.6%. The mitogenome of R.
dabieshanensis shows the typical gene observed in Ranidae
mitogenomes (Liu et al. 2017; Fang et al. 2020; Jiang et al.
2020). Within 37 mitochondrial genes, the ND6 gene and 8
tRNA genes (tRNA
Ser
,tRNA
Glu
,tRNA
Pro
,tRNA
Gln
,tRNA
Ala
,
tRNA
Asn
,tRNA
Cys
and tRNA
Tyr
) were encoded on the light
strand and other genes were encoded by the H-strand. In 13
mitochondrial protein-coding genes, except COX1,ATP6 and
ND4L begin with GTG, ND6 begin with TCT, the other nine
genes begin with ATG as start codon. In all six types of stop
codon were annotated, TAT for ND2, AGG for COX1 and ND5,
TAG for ATP8, TAA for ND4L and CYTB, CAT for ND6 and an
incomplete stop codon T for the remaining six PCGs (COX2,
ATP6,COX3,ND3,ND4 and ND1), which is presumably com-
pleted as TAA by posttranscriptional polyadenylation (Ojala
et al. 1981). With regard to the codon of ND6 and ND2,itis
consistent with the Rana omeimontis submitted on NCBI
(Jiang et al. 2020). Two overlaps among the 13 protein-cod-
ing genes were found: ATP8/ATP6 and ND4L/ND4. The two
rRNA genes were 930 bp (rrnS) and 1576 bp (rrnL),
respectively.
To best understand its phylogenetic position within the
genus Rana, we reconstructed the phylogenetic tree of some
species in Rana with maximum-likelihood method (ML)
(http://iqtree.cibiv.univie.ac.at/), which is based on 13 com-
plete mitochondrial genome sequences for Rana species.
Another species (Pelophylax nigromaculatus) is included as
the outgroup (Figure 1). The result showed that phylogenetic
CONTACT Baowei Zhang zhangbw@ahu.edu.cn School of Life Sciences, Anhui University, Hefei, Anhui 230601, China; Jiaqi Li lijiaqihao@163.com
Environmental Protection Key Laboratory on Biosafety, Nanjing Insititute of Environmental Science, Ministry of Ecology and Environment, Nanjing Jiangsu
210042, China
These authors contributed equally to this work.
ß2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use,
distribution, and reproduction in any medium, provided the original work is properly cited.
MITOCHONDRIAL DNA PART B
2021, VOL. 6, NO. 8, 2418–2419
https://doi.org/10.1080/23802359.2021.1920502

relationship of Dabie mountains wood frog is very close to
R. omeimontis.
Disclosure statement
No potential conflict of interest was reported by the authors.
Funding
This work was supported by the Biodiversity Survey, Monitoring and
Assessment Project of Ministry of Ecology and Environment, China and
2017 Anhui Province Academic and Technical Leaders Candidates
[No. 2017H130].
Data availability statement
The data that support the findings of this study are openly available in
NCBI at https://www.ncbi.nlm.nih.gov/ (MW526989).
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Figure 1. Phylogenetic tree of the relationships of Rana dabieshanensis with maximum-likelihood method (ML) based on complete mitochondrial sequences. The
bootstrap support values for ML analyses are shown orderly on the nodes.
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