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JIMI, Khotso; THUSEHO, Khotso; MOSALA, Malefetsane; PHAKISO, Toma; LEKATSA, Teboho;
HLOKOANE, Oriel
Medicinal Chemistry Research Group, Department of Pharmacy, Faculty of Health Sciences,
National University of Lesotho, P.O. Roma 180, Lesotho
NULISTICE2020 Expo, Pioneer Mall, Maseru, 21-24 January 2020.
Contact Details:
Mr. Oriel K. Hlokoane
Faculty of Health Sciences
Department of Pharmacy
National University of Lesotho
Roma, Lesotho
Phone: (+266) 58146670
Email: hlokoaneo@gmail.com
INTRODUCTON MATERIALS & METHODS
RESULTS & DISCUSSIONS
We thank Mr. Moretloa Polaki of
Department of Biology, National Univeristy
of Lesotho, for plant species authentication.
ACKNOWLEDGEMENTS
Potential Antibiotic Bioprospecting Candidates Leucosidea
sericea and Gazania krebsiana
Acetone/Water (90:10) crude extracts of Leucosidea sericea,H01, and Gazania krebsiana,H23, exhibit great inhibition against
Staphylococcus aureus. No inhibition was observed against strains of Escherichia coli. The relative abundance and tendency of these
two plant species to evade in nature suggest an abundant reservoir of raw materials for potential commercialization.
Lesotho Herbal Medicines
Repository (LHMR)
S. aureus (Gram-positive, skin and upper respiratory tract
pathogenic bacteria) and E. coli (Gram-negative, extra-
intestinal pathogenic bacteria) cause serious infections
such as urinary and respiratory tract infections, skin
infections, and bacteremia. The emergence of Methicillin-
Resistant S. aureus (MRSA) and Multidrug Resistant
(MDR) E. coli is a global concern. The potential of H01
and H23 to address the antibiotic resistance has not yet
been investigated. Our strategies are expected to isolate
and characterize compounds from these two plant
species that have good activities against clinically-
isolated strains of Methicillin-Resistant S. aureus (MRSA)
at concentrations less than 10 µg/mL.
H01
100 g
EXTRACTION
Organic layer,
7.3 g
Acetone/Water
90:10
8.6 %
7.3 %
H23
100 g Organic layer,
8.6 g
Fitoterapia, 91 (2013) 148–153
Successful results against
Staphylococcus aureus
Unsuccessful results
against Escherichia coli
H23 H01 Control
H23 H01 Control
Antibiotic assay
Potential Applications
Antibiotic Cream
Can be used for dressing
septic wounds.
Conclusion
We have studied the antimicrobial activities of two plant species, and
as a result we invented the antibiotic cream that can be used for
dressing of septic wounds.
Future plan
To isolate and characterize compounds from these two plant species
that exhibit good activities against clinically-isolated strains of
Methicillin-Resistant S. aureus (MRSA) at concentrations less than 10
µg/mL.