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enzyme complexes and biosynthesis of kojic acid from Aspergillus. flavus using w:w sucrose:fructose as substrate
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The nanomaterials have important application in different field of science such as biology and pharmacology, which draws the attention of biologists towards this field of study more than before. As the worldwide mortality rate is high due to the pathogens and especially because of the bacteria associated Aeromonas and the antibacterial effect of metal nanoparticles is well known for centuries, these materials can be used to annihilate Aeromonas hydrophila. In this study, silver oxide nanoparticles were synthesized by sol-gel procedure and antibacterial activity of silver oxide nanoparticles as a function of particle concentration against gram-negative bacterium Aeromonas hydrophila ATCC 7966T was carried out in liquid as well as solid growth media. Synthesized Ag 2 O nanoparticles (NPs) were characterized by X-ray diffraction (XRD), scanning electron micrograph (SEM) and transmission electron microscopy (TEM). The average size of the Ag 2 O NPs determined through transmission electron microscopy (TEM) 15 nm. The bactericidal effect of silver oxide nanoparticles was compared based on the diameter of inhibition zone in well diffusion tests in nutrient culture media. Minimum bactericidal concentration (MBC) of nanoparticles dispersed in peptone water, liquid cultures in 22-25 ºC for 24 h were determined.
High-risk human papillomavirus (HPV) infection is the etiological agent of cervical cancer and some other cancers. Kaposi sarcoma-associated herpesvirus (KSHV) represents a principal causative agent of several human cancers arising in those immunocompromised patients. In fact, KSHV DNA has been detected in the female genital tract, and this virus may share some transmission routes with HPV, although the detection rate of KSHV in cervical samples is very low and the KSHV/HPV co-infection is seldom reported. Currently, it remains unclear about the role of KSHV co-infection in the development of HPV-related neoplasias. In this article, we have summarized the recent finding from clinic and bench indicating KSHV co-infection may represent a co-factor for the development of HPV-related carcinogenesis.
The KISS1 gene encodes KISS1, a protein that is rapidly processed in serum into smaller but biologically active peptides called kisspeptins (KPs). KISS1 and the KPs signal via the G-protein coupled receptor KISS1R. While KISS1 and KPs are recognized as potent positive regulators of the reproductive neuroendocrine axis in mammals, the first reported role for KISS1 was that of metastasis suppression in melanoma. Since then, it has become apparent that KISS1, KPs, and KISS1R regulate the development and progression of several cancers but interestingly, while these molecules act as suppressors of tumorigenesis and metastasis in many cancers, in breast and liver cancer they function as promoters. Thus, they join a small but growing number of molecules that exhibit dual roles in cancer highlighting the importance of studying cancer in context. Given their roles, KISS1, KPs and KISS1R represent important molecules in the development of novel therapies and/or as prognostic markers in treating cancer. However, getting to that point requires a detailed understanding of the relationship between these molecules and different cancers. The purpose of this review is therefore to highlight and discuss the clinical studies that have begun describing this relationship in varying cancer types including breast, liver, pancreatic, colorectal, bladder, and ovarian. An emerging theme from the reviewed studies is that the relationship between these molecules and a given cancer is complex and affected by many factors such as the micro-environment and steroid receptor status of the cancer cell. Our review and discussion of these important clinical studies should serve as a valuable resource in the successful development of future clinical studies.
Toll-like receptors (TLRs) play key roles in cerebral ischemia and reperfusion injury by inducing the production of inflammatory mediators, such as interleukins (ILs) and tumor necrosis factor-alpha (TNF-α). According to recent studies, ursolic acid (UA) regulates TLR signaling and exhibits notable anti-inflammatory properties. In the present study, we explored the mechanism by which UA regulates inflammation in the rat middle cerebral artery occlusion and reperfusion (MCAO/R) model. The MCAO/R model was induced in male Sprague–Dawley rats (MCAO for 2 h, followed by reperfusion for 48 h). UA was administered intragastrically at 0.5, 24, and 47 h after reperfusion. The direct high mobility group box 1 (HMGB1) inhibitor glycyrrhizin (GL) was injected intravenously after 0.5 h of ischemia as a positive control. The degree of brain damage was estimated using the neurological deficit score, infarct volume, histopathological changes, and neuronal apoptosis. We assessed IL-1β, TNF-α, and IL-6 levels to evaluate post-ischemic inflammation. HMGB1 and TLR4 expression and phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cell (NFκB) were also examined to explore the underlying mechanism. UA (10 and 20 mg/kg) treatment significantly decreased the neurological deficit scores, infarct volume, apoptotic cells, and IL-1β, TNF-α, and IL-6 concentrations. The infarct area ratio was reduced by (33.07 ± 1.74), (27.05 ± 1.13), (27.49 ± 1.87), and (39.74 ± 2.14)% in the 10 and 20 mg/kg UA, GL, and control groups, respectively. Furthermore, UA (10 and 20 mg/kg) treatment significantly decreased HMGB1 release and the TLR4 level and inactivated NFκB signaling. Thus, the effects of intragastric administration of 20 mg/kg of UA and 10 mg/kg of GL were similar. We provide novel evidence that UA reduces inflammatory cytokine production to protect the brain from cerebral ischemia and reperfusion injury possibly through the HMGB1/TLR4/NFκB signaling pathway.
In this experimental study, first the killing effect of silver nanoparticles alone or in combination with 3mA of Half-Wave Rectified Sine current was assessed in promastigote culture for 10 minutes. The survival rate of infected promastigotes was evaluated by Flow cytometery. In the second step, BALB/c mice were infected experimentally with L. major, followed by silver nanoparticles injected inter-lesion and simultaneously 3 mA a Half-Wave Rectified Sine current induction was applied directly into the wound. Finally, the lesion size and the mice body weight changes were measured during 5 weeks. Results indicated that simultaneous use of nanoparticle and electricity increased the mortality of promastigotes significantly. However, when 3 mA of HWRS and 160 µm/ml nanosilver were used alone in medium culture only 73.4% and 32% of promastigotes were killed respectively but the combined use destroys the promastigotes completely. The diameter of the lesions after six weeks in the control group; group treated with meglumine antimoniate and the group treated with HWRS increased to 6.01, 0.02 and 0.52 mm but in group treated with HWRS plus Nanosilver was reduced to -0.14 mm. The results showed that, when silver nanoparticles with HWRS current electricity were used in mice, the skin lesions were reduced in size but like Glucantime, complete healing was not achieved. © 2018, Malaysian Society for Parasitology. All rights reserved.
This chapter has been extensively revised to address several important issues in the field of cervical neoplasia. These include new information about the origin of cervical squamous neoplasia and its impact on our perceptions of lesion development. This leads in to a discussion of the conundrum of lesion grading, specifically the laboratory management of lesions that fall between cervical intraepithelial neoplasia grade 1 (CIN1) and grade 3 (CIN3). Strategies for managing this problem are offered, and the concept of squamous intraepithelial lesion (SIL) of intermediate (or indeterminate) grade is unveiled. The underpinning of this concept is the absence of any biomarker that can be depended upon to segregate low-grade squamous intraepithelial lesion (LSIL) from high-grade squamous intraepithelial lesion (HSIL) in light of the widespread variations in interpretation between observers and the lack of compelling information to support p16 as either a marker of HSIL or a predictor thereof. Other important topics include new approaches to superficially invasive squamous carcinoma, new management schemes, and the promise of vaccination programs. Finally, the concept of prophylactic ablation of the squamocolumnar junction (SCJ) is addressed as another possible approach to cervical cancer risk reduction in vulnerable populations.
Background:
Pharyngitis is a common disease in the emergency department (ED). Despite a relatively low incidence of complications, there are many dangerous conditions that can mimic this disease and are essential for the emergency physician to consider.
Objective:
This article provides a review of the evaluation and management of group A β-hemolytic Streptococcal (GABHS) pharyngitis, as well as important medical conditions that can mimic this disease.
Discussion:
GABHS pharyngitis often presents with fever, sore throat, tonsillar exudates, and anterior cervical lymphadenopathy. History and physical examination are insufficient for the diagnosis. The Centor criteria or McIsaac score can help risk stratify patients for subsequent testing or treatment. Antibiotics may reduce symptom duration and suppurative complications, but the effect is small. Rheumatic fever is uncommon in developed countries, and shared decision making is recommended if antibiotics are used for this indication. Oral analgesics and topical anesthetics are important for symptom management. Physicians should consider alternate diagnoses that may mimic GABHS pharyngitis, which can include epiglottitis, infectious mononucleosis, Kawasaki disease, acute retroviral syndrome, Lemierre's syndrome, Ludwig's angina, peritonsillar abscess, retropharyngeal abscess, and viral pharyngitis. A focused history and physical examination can help differentiate these conditions.
Conclusions:
GABHS may present similarly to other benign and potentially deadly diseases. Diagnosis and treatment of pharyngitis should be based on clinical evaluation. Consideration of pharyngitis mimics is important in the evaluation and management of ED patients.