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Effect of a chitosan final rinse on the bond strength of root canal fillings
Abstract
This study was designed to evaluate the effect of a final rinse with 0.2% chitosan solution on the adhesion in roots filled with gutta percha and an epoxy resin based sealer. Thirty extracted human maxillary canines selected to ensure specimen standardization were used in the study. After the coronal portion of each tooth was removed, the roots were instrumented and irrigated with 1% sodium hypochlorite (NaOCl). Roots were distributed into 3 groups according to the final rinsing solution (n = 10): 0.2% chitosan, 17% ethylenediaminetetraacetic acid (EDTA), or 1% NaOCl. The canals were irrigated with 5 mL of each solution for 5 minutes and then filled with gutta percha cones and the resin based sealer. Ten roots in each group were prepared, sectioned, and submitted to push-out testing. Data were analyzed with analysis of variance and Tukey test (P < 0.05). In the push-out test, final irrigation with chitosan (mean, 0.37 [SD, 0.12] MPa) or EDTA (0.38 [0.11] MPa) resulted in significantly greater bond strength of the sealer to the root canal (P < 0.05) than did irrigation with 1% NaOCl (0.13 [0.04] MPa). The cervical third had greater bond strength than the other thirds (P < 0.05). Adhesive failure was the most frequent type in all groups. A final rinse with 0.2% chitosan or 17% EDTA resulted in greater bond strength of root fillings to the root canal than did 1% NaOCl.
... 7 Previous studies have shown that chitosan increases the bond strength between the root canal sealer and dentinal tubules. 6,8 Silver nanoparticles (AgNPs) are a new material with unique surface properties and high antimicrobial efficacy. 9 AgNPs have become popular because they release silver ions, which cause bacterial cell deterioration. ...
Background. This study evaluated the combined effects of silver nanoparticles (AgNPs) and chitosan on the dentin bond strength of resin-based root canal sealers using the push-out test and scanning electron microscopy (SEM).
Methods. This in vitro study was conducted on 72 extracted mandibular premolar teeth. All the teeth were decoronated perpendicular to the long axis to leave a 13-mm root length. The root canals were prepared, and the samples were randomly divided into seven experimental groups and one control group based on final irrigation solutions. All the final irrigation procedures were performed for one minute. The root canals were dried using paper points and filled with a resin-based sealer and gutta-percha points using a lateral condensation technique. Sections measuring 2 mm in thickness were taken from the apical, middle, and coronal thirds of each root using a cutting machine. The push-out test was performed using a universal testing machine.
Results. The solution of AgNPs combined with 0.4% chitosan showed higher bond strength in the coronal region than a combination with 0.2% chitosan. Samples treated with 0.4% chitosan solution exhibited a higher bond strength than the 0.2% chitosan group. There were no significant differences between chlorhexidine (CHX) solution alone and in combination with 0.2% or 0.4% chitosan solution.
Conclusion. The combination of chitosan and AgNPs was as effective as CHX in improving the bond strength of resin-based sealers.
... The use of chitosan extracts has been highlighted in several areas of medicine and dentistry [6][7][8]. Chitosan is a biopolymer obtained from the deacetylation of chitin and naturally occurs in the cell walls of fungi, yeasts, insects, and mainly in crustaceans' shells [9]. It is a promising material, biocompatible, biodegradable, non-toxic and bioadhesive, in addition to furthering teeth remineralization through calcium and phosphate deposition. ...
Objectives:
Metalloproteinase-inhibiting agents, such as chitosan, can prevent collagen degradation in demineralized dental substrates, thereby improving the adhesive interface. This study evaluated the bond strength (BS) and chemical and morphological characterization of the adhesive interface after applying chitosan solution to demineralized dentin.
Materials and methods:
The 80 third molars were selected. Forty teeth underwent caries induction using the pH cycling method. The teeth were divided according to the treatment: distilled water (control) and 2.5% chitosan solution. The surfaces were restored using adhesive and composite resins. Half of the specimens in each group were aged, and the other half underwent immediate analyses. The teeth were sectioned and underwent the microtensile bond strength test (µTBS), and chemical and morphological analyses using energy-dispersive spectroscopy and scanning electron microscopy, respectively. Data analysis was performed using 3-way analysis of variance.
Results:
For µTBS, sound dentin was superior to demineralized dentin (p < 0.001), chitosan-treated specimens had higher bond strength than the untreated ones (p < 0.001), and those that underwent immediate analysis had higher values than the aged specimens (p = 0.019). No significant differences were observed in the chemical or morphological compositions.
Conclusions:
Chitosan treatment improved bond strength both immediately and after aging, even in demineralized dentin.
... Moreover, sealer penetration results were in agreement with Aydın et al (2019) (39) , who concluded that EDTA enhanced sealer penetration that was superior to chitosan nanoparticle penetration. On the other hand, previous researches contradicted this study's findings, which reported that 0.2% chitosan provided the same bond strength as 17% EDTA (26,31,56) and similar sealer penetration at the middle and apical thirds (31) . ...
Purpose: To evaluate the effect of 3% green tea extract (GTE) on wettability, depth of penetration, and push out bond strength (PBS) of AH Plus sealer to intraradicular dentin following the use of 0.2% Nano chitosan or 17% EDTA. Materials and methods: Twenty human radicular dentin segments were used for wettability assessment. Following immersion in 2.6% NaOCl solution, samples were divided into 2 groups (n=10) according to the chelating agent used: group I: 0.2% Nano chitosan, and group II: 17% EDTA, each used for 3 min, then the contact angle (CA) was measured. The samples were further immersed in 3% GTE solution for 5 min followed by CA measurement. Twenty human mandibular premolars were prepared up to # X4 ProTaper Next files, then divided into two groups as mentioned previously. Each group was subdivided into two subgroups based on the final flush used (n=5); subgroups A and B; no treatment, and 3% GTE, respectively. Samples were obturated by cold lateral compaction using AH Plus sealer and gutta-percha. PBS was measured using a universal testing machine and sealer penetration was assessed using confocal laser scanning microscopy. Results: Using 3% GTE following the use of 0.2% Nano chitosan significantly increased the wettability and PBS of AH Plus sealer to intraradicular dentin, while its use following 17% EDTA significantly decreased the wettability and PBS of AH Plus sealer. Using GTE increased the maximum depth of AH Plus sealer penetration into the dentinal tubules. Conclusion: GTE improved the wettability, depth of penetration, and push out bond strength of AH Plus sealer to intraradicular dentin treated with Nano-chitosan. Nevertheless, it adversely affected those properties of AH Plus sealer to EDTA-treated dentin.
... After the analysis of titles and abstracts, 23 articles were selected for full-text reading. After comprehensive reading of these studies, 11 were excluded because they did not present a control group without a chelating agent (17)(18)(19)(20)(21)(22)(23)(24)(25), used different push-out methodology (26) or did not use human teeth (27). Therefore, 12 studies fulfilled the eligibility criteria and were included in this systematic review. ...
This systematic review (PROSPERO – CRD42020150722) was performed to answer the following question: Does the use of chelating agents affect the dislodgment resistance of epoxy resin-based root canal sealers? A search was conducted in PubMed, EMBASE, Scopus, LILACS and Web of Science, hand searching of two endodontic journals and grey literature until September 2020. Extracted data included: teeth used, sample size, root canal preparation, irrigant, chelating solution, neutralisation solution, type of resin-based sealer and obturation approach, storage method and duration, root third and slice thickness, plunger dimension and loading direction and the push-out testing parameters and values. The search retrieved a total of 2.389 studies. After analysis, 12 studies fulfilled the eligibility criteria and were included. Most of the final irrigation protocols with chelating agents had a positive impact and promoted an improvement in the dislodgment resistance of epoxy resin-based sealers to the root dentin.
... e cleanliness of the root canal wall from the smear layer increases bond strength to dentin and reduces microleakage for sealers by enhancing the sealer penetration into the dentinal tubules resulting in mechanical interlocking to the canal walls [8]. Several previous studies were in accordance with this study which stated that 0.2% chitosan produced the same bond strength as 17% EDTA [34,35] and greater sealer penetration with a variety of application times [36,37]. e advantage of chitosan compared to EDTA as the final irrigation material is chitosan has antibacterial properties, while EDTA has no antibacterial properties [16], although antibacterial properties of chitosan were not tested in this study. ...
Materials and methods:
Fifty-six premolars were used in this study and divided by two evaluations: 28 teeth for apical sealing ability and 28 others for bond strength. Each study was assigned randomly into two groups of fourteen teeth: Group-1, final irrigation with 17% EDTA; Group-2, with 0.5% chitosan nanoparticles. Each group was further divided into two groups of 7 each: Group-A, final irrigation was applied for 1 minute; Group-B, for 3 minutes. All teeth were obturated with epoxy resin-based sealer and gutta-percha. In the apical sealing ability study, the obturated teeth were immersed in 2% methylene blue and observed under a stereomicroscope (8x magnification). In the bond strength study, the teeth were tested using the push-out technique and observed under a stereomicroscope (40x magnification) to determine the failure type. Data from each evaluation were analysed with two-way ANOVA followed by the LSD test.
Results:
Final irrigation using 0.5% chitosan nanoparticles produced the same apical sealing ability and bond strength as 17% EDTA (p > 0.05). A significant difference occurred between application times (p < 0.05). The failure type was observed predominantly as cohesive, and the least was adhesive.
Conclusion:
Regardless of the final irrigation solution used, 3-minute application time produced greater apical sealing ability and push-out bond strength than 1-minute application time.
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