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The complete mitochondrial genome of Paecilomyces penicillatus (Hypocreales: Sordariomycetes)

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Mitochondrial DNA Part B
Authors:
Chen Cheng at Zhejiang University
Chen Cheng
Qiang Li at Chengdu University
Qiang Li
Haiying Chen
Haiying Chen
Haiying Chen
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Rongtao Fu
Rongtao Fu
Rongtao Fu
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Abstract and Figures

Paecilomyces penicillatus is the causative pathogens of white mould on cultivated Morchella. Here, we report the complete mitochondrial genome of P. penicillatus for the first time. The mitogenome is composed of circular DNA molecules, with the total length of 27,480 bp, which encoded 17 protein-coding genes (PCGs), 2 ribosomal RNA genes (rRNA), and 24 transfer RNA (tRNA) genes. The base composition of the P. penicillatus mitogenome is as follows: A (36.69%), T (35.79%), G (15.27%), and C (12.25%). Phylogenetic analysis revealed that P. penicillatus had a close relationship with Epichloe festucae and Epichloe typhina from Clavicipitaceae. This study provided important information on the evolution and taxonomy of P. penicillatus.
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MITOGENOME ANNOUNCEMENT
The complete mitochondrial genome of Paecilomyces penicillatus (Hypocreales:
Sordariomycetes)
Cheng Chen
a
, Qiang Li
a
, Haiying Chen
b
, Rongtao Fu
a
, Jian Wang
a
, Xuejuan Chen
a
, Rongping Hu
a
and
Daihua Lu
a
a
Institute of plant protection, Sichuan Academy of Agricultural Sciences, Chengdu, P.R. China;
b
Bazhou Soil and Fertilizer Workstation,
Bazhong, P.R. China
ABSTRACT
Paecilomyces penicillatus is the causative pathogens of white mould on cultivated Morchella. Here, we
report the complete mitochondrial genome of P. penicillatus for the first time. The mitogenome is com-
posed of circular DNA molecules, with the total length of 27,480bp, which encoded 17 protein-coding
genes (PCGs), 2 ribosomal RNA genes (rRNA), and 24 transfer RNA (tRNA) genes. The base composition
of the P. penicillatus mitogenome is as follows: A (36.69%), T (35.79%), G (15.27%), and C (12.25%).
Phylogenetic analysis revealed that P. penicillatus had a close relationship with Epichloe festucae and
Epichloe typhina from Clavicipitaceae. This study provided important information on the evolution and
taxonomy of P. penicillatus.
ARTICLE HISTORY
Received 19 October 2018
Accepted 20 October 2018
KEYWORDS
Paecilomyces penicillatus;
mitogenome;
phylogenetic analysis
The genus Paecilomyces was established by Bainier, which
was considered to be similar to Penicillium (Bainier 1907).
Luangsa-Ard et al. (2004) demonstrated that Paecilomyces
was polyphyletic across two subclasses (Sordariomycetidae
and Eurotiomycetidae) through phylogenetic analysis of the
18S rRNA. Inglis and Tigano (2006) further supported the
polyphyly of the genus through phylogenetic analysis of the
5.8S rRNA and internal transcribed spacer (ITS1 and ITS2)
sequences from some Paecilomyces species. Paecilomyces pen-
icillatus was originally isolated from rotten mushrooms
(Samson 1974). He et al. (2017) demonstrated that P. penicil-
latus was the causative pathogen of morels which produced
white mold-like symptoms on the caps and stripes of
Morchella. Furthermore, P. penicillatus was not monophyletic,
which had an uncertain affinity with the Clavicipitaceae
within the Hypocreales (Luangsa-Ard et al. 2005). The
mitogenome of P. penicillatus reported here would provide
important information on the evolution and taxonomy of
P. penicillatus.
The strain was isolated from the diseased fruiting body of
cultivated morels from Chengdu, Sichuan Province, China
(103.87 E; 30.68 N). Koch’s postulates, morphological features,
and ITS sequences analysis showed that the strain was identi-
cal to the causative agent of white mould on cultivated
Morchella (He et al. 2017). The specimen (P. penicillatus) was
stored in Sichuan Academy of Agricultural Sciences (No.
SAAS_ppe1). Total DNA was extracted from the mycelia using
the fungal DNA Kit D3390-00 (Omega Bio-Tek, Norcross, GA)
according to the manufacturer’s instructions. Purified DNA
was used to construct the sequencing libraries following the
instructions of NEBNext Ultra II DNA Library Prep Kit (NEB,
Beijing, China). Mitochondrial (mt) genome sequencing was
performed using an Illumina HiSeq 2500 Platform (Illumina, San
Diego, CA). We performed quality control and de novo assem-
bly of the mitogenome according to Bi (2017). The SPAdes 3.9.0
(Bankevich et al. 2012) was used to de novo assemble the
mitogenome; the MITObim 1.9 (Hahn et al. 2013) was used to
fill the gaps between contigs. The MFannot (http://megasun.
bch.umontreal.ca/cgi-bin/mfannot/mfannotInterface.pl)andMITOS
(Bernt M et al. 2013) tool were used for mt genome annotation of
P. penicillatus. The tRNAscan-SE 2.0 (Lowe and Chan 2016)wasused
to predict tRNA genes.
The mitogenome of P. penicillatus was assembled as circular
DNA molecules with 27,480 bp in length, which encoded 17
protein-coding genes (PCGs), 24 tRNA genes, and 2 ribosomal
RNA genes (rnl and rns). The overall base composition of P.
penicillatus is as follows: A (36.69%), T (35.79%), G (15.27%), and
C (12.25%). The mitogenome of P. penicillatus was submitted to
GenBank database under Accession No. MK069583.
The Bayesian inference (BI) methods using the MrBayes
version 3.2.6 (Ronquist et al. 2012) software were performed
for phylogenetic analysis of P. penicillatus and 17 closely
related species. The 15 typical PCGs (14 conserved core PCGs
and rps3) were used to construct the phylogenetic trees, and
the node support was calculated according to Bayesian pos-
terior probabilities (BPP) (Figure 1). The phylogenetic analysis
revealed that P. penicillatus has a close relationship with
Epichloe festucae and Epichloe typhina from Clavicipitaceae.
CONTACT Daihua Lu 453831354@qq.com Institute of plant protection, Sichuan Academy of Agricultural Sciences, 20 # Jingjusi Rd, Chengdu 610066,
Sichuan, P.R. China
ß2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use,
distribution, and reproduction in any medium, provided the original work is properly cited.
MITOCHONDRIAL DNA PART B
2019, VOL. 4, NO. 1, 199–200
https://doi.org/10.1080/23802359.2018.1545549
Disclosure statement
No potential conflict of interest was reported by the authors.
Funding
This research was funded by the Foundation Program of the Financial &
Innovational Capacity Building Project of Sichuan [2018QNJJ-013 &
2016GYSH-014] and Science and Technology Support Project of Sichuan
Province [2016NYZ0053].
References
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Figure 1. Molecular phylogenies of 18 species based on Bayesian inference analysis of the combined mitochondrial gene set (15 core protein-coding genes). Node
support values are Bayesian posterior probabilities (BPP). Mitogenome accession numbers used in this phylogeny analysis: Epichloe festucae (NC_032064), Epichloe
typhina (NC_032063), Metacordyceps chlamydosporia (NC_022835), Metarhizium anisopliae (NC_008068), Trichoderma reesei (NC_003388), Fusarium circinatum
(NC_022681), Fusarium mangiferae (NC_029194), Fusarium verticillioides (NC_016687), Fusarium commune (NC_036106), Fusarium oxysporum (NC_017930), Fusarium
culmorum (NC_026993), Fusarium gerlachii (NC_025928), Fusarium graminearum (NC_009493), Fusarium solani (NC_016680), Ilyonectria destructans (NC_030340),
Ilyonectria sp. (MF924828), Nectria cinnabarina (NC_030252).
200 C. CHEN ET AL.
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