Abstract In the present paper, a group of species separated from Chrysotus Meigen, 1824 is establishied as new genus, Dubius gen. nov., of which five new species from Guizhou, Southwestern China are described: D. autumnalus sp. nov., D. curtus sp. nov., D. frontus sp. nov., D. hongyaensis sp. nov. and D. succurtus sp. nov. It may be reasonable conjecture that Dubius gen. nov. derives from the Southern land (Gondwana land) and spreads to Oriental Region because of the new genus is only found in Neotropical Realm and Southwestern China belonging to Oriental Region.