Supplementary Material

Abstract

Notes S1
Laccaria Systematics and Global Laccaria phylogenies with collection ID numbers.

Notes S2 Specimen information and GenBank sequence IDs.

Notes S3 Agaricomycetideae phylogeny and calibration priors for time to MRCA analysis.

Notes S4 Agaricomycetideae and Global Laccaria time to MRCA results.

Notes S5 BAMM results.

Notes S6 BiSSE results.

Notes S7 Isotope data and specimen information.

Full text

New Phytologist Supporting Information (Notes)
Article title: Evolution of ectomycorrhizas as a driver of diversification and biogeographic
patterns in the model mycorrhizal mushroom genus Laccaria
Authors: Andrew W. Wilson, Kentaro Hosaka, Gregory M. Mueller
Article acceptance date: 15 September 2016
The following Supporting Information is available for this article (click on underlined item
to jump to page):
Notes S1a – Figure S1a: Laccaria Systematics phylogeny and exemplary
images of Laccaria taxa.
S1b – Figure S1b: Global Laccaria phylogeny with sample IDs.
Notes S2a – Table S2a: Specimen Information and GenBank IDs
S2b – Table S2a: Agaricomycetideae dataset sequence data
S2c – Table S2c: Dataset length and composition information
Notes S3 – tMRCA priors and Agaricomycetideae phylogeny results
Notes S4a – Table S4a: Agaricomycetideae tMRCA results and
comparison of molecular dating results
S4b – Table S4b: Global Laccaria tMRCA results
Notes S5 –BAMM results: Global Laccaria diversification
Notes S6 – BiSSE results: Northern vs Southern Hemisphere Laccaria
Notes S7 – Isotope data and specimen information.

PNG3
PNG5
Laccaria acanthospora
Laccaria alba complex
Laccaria pseudomontana
Laccaria gomezii
Laccaria bicolor
pan-beringia
bicolor
Laccaria bicolor complex Laccaria amethystina complex
Laccaria
amethystina
Laccaria
aff amethystina1
Laccaria
aff amethystina2
Laccaria ocrhropurpurea
Laccaria bullipellis
Laccaria
himalayensis
Laccaria
vinaceobrunea
Laccaria
amethysteo-occidentalis
Laccaria trullisata
Laccaria angustilamella
Laccaria longipes
Laccaria
vinaceoavellanea
THAI1
THAI2
THAI3
THAI4
proxima 1
Laccaria major
PORT2
Laccaria salmonicolor
JP3
JP2
Laccaria aff
negrimartinata
JP1
PORT1
Laccaria
macrocystidia
Laccaria
montana/pumila
complex
CHINA1
South asian Laccaria
complex
THAI5
THAI6
CHINA2
“laccata”
Laccaria aff tortilis
JP4
Laccaria aurantia
Laccaria fulvogrisea
Notes S1a
Laccaria Systematics Phylogeny (DS1)
Maximum Likelihood Tree (RAxML)
4 genes: ITS, 28S, RPB2, EF1a
(Available sequences are indicated on branch label, respectively.
Refer to Notes S2 for GenBank accession numbers.
A “0” indicates sequence not available for that gene.)
ntaxa=237 (232 ingroup)
Numbers on branches:
whole numbers = ML bootstrap % (1000 replicates)
fractions = Posterior Probabilities (10 million gen)
Branch labels in black identify samples used
in tMRCA analysis of the Global Laccaria Dataset
(Figure 1 & Notes S1b).
To Southern
Hemisphere
Laccaria
PRL4777 USA: IL ITS 28S RPB2 0
L alba GMM6131 China JX504131 JX504210 RPB2 EF1a
L amethystina GMM7041 ITS 28S RPB2 0
L nobilis AWW584 USA: OR/BentonCo JX504110 JX504193 RPB2 0
L amethystina KH LA06 002 USA: LA ITS 28S RPB2 EF1a
L amethystina F1102432 CostaRica ITS 28S RPB2 0
L proxima GMM7020 Russia: Caucasus ITS 28S RPB2 0
L sp A3344 China ITS 0 0 0
L proxima GMM7628 France: Forest-comaniale ITS 28S RPB2 EF1a
L sp AWW596 USA: AK JX504116 JX504199 0 0
L trichodermophora GMM7733 USA: TX/Tyler ITS 28S RPB2 0
L ohiensis GMM7028 Russia: Caucasus ITS 28S RPB2 0
TYPE Cripps1625 USA: CO DQ149871 0 0 0
L sp A0576 Jap: Tsu ITS 0 0 0
L sp TWO1178 Thai: ChaingMai ITS 28S RPB2 EF1a
L sp AWW583 USA: OR/BentonCo ITS 28S 0 0
L amethysteo-occidentalis F1068333 Wisconsin ITS 28S RPB2 0
L sp GMM6776 China: Yunnan ITS 28S RPB2 EF1a
L proxima F1133825 USA: MS ITS 28S RPB2 0
L sp A0561 Jap: Sap JX504082 0 0 0
F1061267 Japan: Tottori JX504121 0 0 0
L sp A0087 Jap: Fujikawa ITS 0 0 0
AWW463 China: Tibet/Linzhi JX504098 JX504182 RPB2 0
L tortilis GMM7635 France: Forest-comaniale ITS 28S RPB2 EF1a
L sp AWW569 USA: MI/UpPeninsula JX504108 28S RPB2 0
TYPE AWW465 China: Tibet/Linzhi JX504100 JX504184 RPB2 0
L sp TWO1150 Thailand ITS 28S RPB2 0
A2986 JAP: Fushimi JN942810 JN939738 JN993520 0
AFTOL447 0 AY700200 DQ472731 0
L amethystina AB211271 Japan AB211271 0 0 0
L amethystina GMM7167 ITS 28S 0 EF1a
L bicolor F1121424 China JX504127 JX504207 RPB2 0
L amethystina F1123822 Wisconsin ITS 28S RPB2 0
L sp TWO1184 Thai: ChaingMai ITS 28S 0 EF1a
L amethystina GMM7621 France: Forest-comaniale ITS 28S RPB2 EF1a
L trichodermophora F1111951 CostaRica ITS 28S RPB2 0
L sp GMM6595 China: Yunnan ITS 0 RPB2 0
L sp GMM7630 China: Tibet ITS 28S RPB2 EF1a
L sp DED7419 Thailand ITS 28S RPB2 EF1a
L macrocystidia GMM7616 France: Vallon ITS 28S RPB2 0
L sp A0573 Jap: Nar ITS 0 0 0
L bicolor SB2238 Portugal ITS 28S RPB2 0
L major GMM6012 ITS 28S RPB2 0
L sp DED7435 Thailand ITS 28S RPB2 0
L sp AWW567 USA: MI/UpPeninsula ITS 28S 0 0
L cf laccata A2987 JAP: Fushimi JN942786 JN939739 JN993521 0
L trichodermophora KH_LA06_013 USA: LA ITS 28S 0 0
L proxima GMM7584 Russia: Komorav ITS 28S RPB2 EF1a
L bicolor S238N JGI/Genome DQ179123 0 RPB2 EF1a
L trichodermophora GMM7712 USA: TX/Newton ITS 0 RPB2 0
PRL3777 USA: IL ITS 28S RPB2 0
L sp TWO1166 Thai: ChaingMai ITS 28S RPB2 0
TYPE AWW485 China: Tibet/Linzhi JX504102 JX504186 RPB2 EF1a
L bicolor GMM6094 ITS 28S RPB2 0
Cripps1771 USA: CO DQ149870 0 0 0
L nobilis F1091206 USA: MI ITS 28S 0 0
L sp GMM6800 ITS 28S RPB2 EF1a
AWW556 USA: Ca/NevadaCity JX504107 JX504191 RPB2 0
L laccata SB2067 Portugal ITS 28S RPB2 0
KH_LA06_016 USA: LA ITS 0 0 0
L sp HKAS44062 China: Yunnan ITS 28S RPB2 0
L sp A0051 Jap: Nar ITS 0 0 0
TYPE AWW484 China: Tibet/Linzhi JX504101 JX504185 RPB2 0
L sp A0556 Jap: Mako ITS 0 0 0
L amethystina LaAM_08_1 JGI/Genome ITS 28S RPB2 EF1a
L major GMM6019 CostaRica ITS 28S RPB2 EF1a
L amethystina ALB183 Tibet JX504092 JX504176 RPB2 EF1a
L sp TWO1194 Thai: Prajenburi ITS 28S 0 EF1a
L pumila GMM7637 France: FraychiredeBog ITS 28S 0 EF1a
L sp A1800 Taiwan ITS 0 0 0
L longipes F1092175 USA: MI ITS 28S 0 0
L laccata s.l. GMM7586 Russia: Komorav ITS 28S RPB2 0
L laccata SB2135 Portugal ITS 28S RPB2 EF1a
L sp GMM7627 France: Forest-comaniale 0 28S RPB2 EF1a
L sp GMM7613 France: Vallon ITS 28S RPB2 EF1a
L trullisata PRL7587 ITS 28S RPB2 EF1a
L alba HKAS42577 China: Yunnan 0 JX504234 0 0
L sp AWW572 USA: MI, UpPeninsula ITS 28S RPB2 0
L ohiensis KH_07192006_1 USA: IN ITS 28S RPB2 0
L cf laccata A0553 JAP: Makomanai JN942780 JN939733 JN993511 0
L aff montana AWW446 China: Tibet JX504097 JX504181 RPB2 EF1a
L sp HKAS57950 China: Tibet ITS JX504242 0 0
L sp GMM7625 France: Forest-comaniale 0 28S RPB2 EF1a
L vinaceobrunnea F1110429 USA: TX 0 28S 0 0
L macrocystidia GMM7626 France: Forest-comaniale ITS 28S RPB2 EF1a
L bicolor_proxima GMM7631France France: Forest-comaniale ITS 28S RPB2 EF1a
L macrocystidia GMM7612 France: Vallon ITS 28S RPB2 EF1a
L gomezii GMM6022 ITS 0 0 0
L laccata SB2210 Portugal ITS 28S RPB2 EF1a
L sp GMM6723 ITS 28S RPB2 0
L sp DED7426 Thailand ITS 28S RPB2 EF1a
L salmonicolor GMM7596 China: Tibet ITS 28S RPB2 EF1a
L calospora F1104722 CostaRica ITS 28S 0 0
L sp F1120731 China JX504125 JX504205 0 0
L PNG3 GMM7302 PNG JX504138 JX504214 RPB2 GMM7302
L sp TWO1168 Thai: ChaingMai ITS 28S RPB2 EF1a
L sp GMM6679 China: Yunnan ITS 28S RPB2 EF1a
L sp ZT9196 Indonesia: Lombok ITS 28S RPB2 0
L pumila AF287869 0 AF287869 0 0
L albaTYPE HKAS41457 China: Yunnan, Longling 0 JX504232 0 0
L angustilamellaTYPE HKAS41483 China: Yunnan, Longling 0 JX504233 0 0
L trullisata WCG2072 ITS 0 RPB2 EF1a
L PNG5 OSC122484 PNG OSC122484 0 0 0
L sp A0577 Jap: Iwa ITS 0 0 0
L laccata GMM6172 ITS 28S RPB2 0
L sp A0584 Jap: Mako ITS 0 0 0
L sp GMM6585 ITS 28S RPB2 0
L vinaceobrunea KH_LA06_018 USA: LA 0 28S RPB2 0
L bicolor SB2239 Portugal ITS 28S RPB2 0
L tortilis F1116205 USA: IL ITS 28S 0 0
L aff ohiensis AWW545 USA: IL JX504106 JX504190 RPB2 0
L laccata GMM7605 France: Vallon ITS 28S RPB2 EF1a
L alba F1120750 China JX504166 JX504242 0 0
L laccata SB2133 Portugal ITS 28S RPB2 EF1a
L negrimarginataTYPE BAP360 China: Tibet JX504120 0 0 0
L proximella F1081079 Argentina: Neuquen ITS 28S RPB2 0
L amethystina AB211270 Japan AB211270 0 0 0
L cf laccata A3394 JAP: Suzuka JN942788 JN939770 JN993522 0
L sp3 GMM7301 PNG 0 28S RPB2 EF1a
L sp AWW591 USA: OR, BentonCo 0 28S RPB2 0
L bicolor F1091205 ITS 0 0 0
L sp A0585 Jap: Mako ITS 0 0 0
L aff bicolor AWW539 USA: IL ITS 28S 0 0
L bicolor F1077767 ITS 28S 0 0
HKAS45998 China: Tibet ITS 28S RPB2 0
L AWW555 USA: Ca, NevadaCity 0 28S RPB2 EF1a
L sp GMM7631Tibet China: Tibet JX504152 JX504226 RPB2 EF1a
L sp GMM6623 China ITS 28S RPB2 EF1a
AWW590 USA: OR, BentonCo JX504112 JX504195 RPB2 0
L alba AWW438 China: Yunnan, Shangrila JX504094 JX504178 RPB2 EF1a
12 13
14
15
16
17
18
19
20
99/
0.99
100/1.0
96
100/
1.0
97/
1.0
99/1.0
86
91/
0.99
100/1.0
85/
0.99
70/
0.99
92
100/1.0
100/1.0
99
100/1.0
100/1.0
100
100/1.0
100
88/1.0
100/1.0
96/1.0
99/
1.0
100/
1.0
100/1.0
71
100/1.0
70
97/
1.0
97/
0.98
76/0.97
93/1.0
100/1.0
83/
1.0
100
75/1.0
83/1.0
99/0.99
99/
0.98
89/0.99
97
91/1.0
88/0.99
88/1.0
87/1.0
100/1.0
97/
0.99
100/1.0
99/
1.0
99/
1.0
100/
1.0
99/1.0
94/
1.0
98/1.0
92/1.0
99/
0.99
100/
1.0
100/1.0
70
80
99/0.99
86/0.99
88/
0.99
0.96
0.97
0.99
0.96
F
G
H
J
Laccaria laccata
G.M. Muller
Laccaria vinaceobrunea
G.M. Muller
Laccaria
amethystina
G.M. Muller
Laccaria
bicolor
A.W. Wilson

Q1
NZ2
SpE1
SpA
NZ3
B5
H. sublamellatum
B2
MAS1
SpS
Arg 1
Arg 2
PNG2
PNG4
SpB1
SpC2
CAN5
MAS2
NZ1 complex
masonii/fibrillosa/lilacina
complex
Q2
HCAR2
LAT3
CAN3
PNG1
LAT2
NZ4
SpD
LAT1
LAT1a
CAN4
Laccaria glabripies
NZ6
HCAR1
NZ8
NZ7
NZ10
Hydnangium kanuka
CAN2
CAR4
CAN1
CAN6
Laccaria galerinoides
To Northern Hemisphere
and Tropical Laccaria
L masonii GMM7473 NZ: Buller ITS 28S RPB2 EF1a
L spA GMM7403 Aus: Qland ITS 28S RPB2 EF1a
L violaceoniger GMM7533 NZ: Otago ITS 28S RPB2 0
L spE GMM7440 Aus: Tas ITS 28S RPB2 EF1a
L canaliculata GMM7276 Aus: Vic ITS 28S RPB2 EF1a
L spD GMM7217 Aus: Vic ITS 28S RPB2 EF1a
L spE GMM7213 Aus: Vic ITS 28S RPB2 EF1a
L sp KH_NZ06_197 NZ: Waitakere ITS 28S RPB2 EF1a
L violaceoniger GMM7580 NZ: Southland ITS 28S RPB2 0
L B2 MEL2359670 JX270726 0 JX295596 JX305610
L canaliculata GMM7205 Aus: Vic ITS 28S RPB2 EF1a
L spC GMM7212 Aus: Vic ITS 28S RPB2 0
L sp KH_NZ06_072 NZ: Coromandel ITS 0 RPB2 0
L spB GMM7257 Aus: Vic ITS 28S RPB2 EF1a
L spB GMM7265 Aus: Vic ITS 28S RPB2 EF1a
L galerinoides A2103 Argentina ITS 0 0 0
L spE GMM7454 Aus: Tas ITS 0 0 0
L lilacina GMM7531 NZ: Otago ITS 28S RPB2 EF1a
L spB GMM7254 Aus: Vic ITS 28S 0 0
H carneum Trappe27650 Aus: NSW ITS 28S RPB2 0
L glabripes GMM7534 NZ: Otago ITS 28S 0 0
L masonii GMM7200 Aus: Vic ITS 28S RPB2 EF1a
L canaliculata GMM7264 Aus: Vic ITS 28S RPB2 0
Inocybe cookei AFTOL520 DQ404391 AY702014 DQ385884 DQ435790
L CAN2 MEL2359684 JX270714 0 JX295587 JX305600
L CAN4 MEL2359686 Aus: Tas JX270723 0 0 JX305622
L violaceoniger GMM7520 NZ: Otago ITS 28S RPB2 0
L ohiensis GMM7521 NZ: Otago ITS 28S RPB2 EF1a
H carneum Trappe31270 Aus: NSW ITS 28S RPB2 EF1a
L galerinoides F1081213 Chile ITS 28S RPB2 EF1a
L lateritia GMM7220 Aus: Vic ITS 28S RPB2 0
L CAN3 MEL2359685 JX270697 0 JX295600 JX305613
H carneum GMM7272 Aus: Vic ITS 28S RPB2 EF1a
L fibrillosa GMM7508 NZ: Karamea ITS 28S RPB2 0
H HCAR2 MEL2359676 JX270733 0 0 JX305633
H sp Trappe31056 Aus: Vic ITS 0 RPB2 EF1a
Coprinus comatus AFTOL626 AY854066 AY635772 AY780934 AY881026
L B3 MEL226520 JX270713 0 JX295628 JX305639
L sp A2064 Argentina ITS 0 0 0
L lateritia/sensuMay/ GMM7221 Aus: Vic ITS 28S RPB2 0
L white/palepinkish A2111 Argentina ITS 0 0 0
L lateritia/sensuMay/ GMM7250 Aus: Vic ITS 28S RPB2 0
L violaceoniger A0492 New/Zealand ITS 0 0 0
L laccata GMM7411 Aus: Qland ITS 28S RPB2 EF1a
L spD GMM7240 Aus: Vic ITS 28S RPB2 0
L spA GMM7402 Aus: Qland ITS 28S RPB2 EF1a
L spA GMM7226 Aus: Vic ITS 28S RPB2 EF1a
H sublamellatum MEL2297141 AUS: WA ITS 28S RPB2 EF1a
L sp KH_NZ06_119 NZ: TeToto ITS 28S RPB2 EF1a
L canaliculata GMM7227 Aus: Vic ITS 28S RPB2 EF1a
L canaliculata GMM7251 Aus: Vic ITS 28S RPB2 EF1a
H carneum GMM7449 Aus: Tas ITS 28S RPB2 EF1a
L sp2 GMM7282 PNG ITS 28S RPB2 EF1a
L canaliculata GMM7222 Aus: Vic ITS 28S RPB2 0
L masoni GMM7416 Aus: Tas ITS 28S RPB2 EF1a
L galerinoides F1080983 Argentina ITS 28S RPB2 EF1a
L spA GMM7457 Aus: Tas ITS 28S RPB2 0
H carneum Trappe27676 Aus: Vic ITS 0 0 0
L sp KH_NZ06_122 NZ: TeToto ITS 28S 0 EF1a
H carneum GMM7273leptospermum Aus: Vic ITS 28S RPB2 EF1a
Mythicomyces corneipes AFTOL972 DQ404393 AY745707 AFTOL972 DQ029197
H sp Trappe14544 Aus: Tas ITS 28S RPB2 0
L spA GMM7422 Aus: Tas ITS 28S RPB2 EF1a
L sp GMM5935 ITS 28S RPB2 0
L spB GMM7274 Aus: Vic ITS 28S RPB2 EF1a
L sp4 GMM7316 PNG ITS 28S RPB2 EF1a
L canaliculata GMM7458 Aus: Tas ITS 0 0
L masoni GMM7420 Aus: Tas ITS 28S RPB2 0
L ambigua PDD89696 NZ: Coromandel Peninsula KauriGrove ITS 28S RPB2 EF1a
L Lat3 GMM7207 Aus: Vic ITS 28S RPB2 0
L sp1 GMM7285 PNG ITS 28S RPB2 0
L sp Syme1586_06 AUS: WA ITS 28S RPB2 EF1a
L sp A2062 Argentina ITS 0 0 0
L spB GMM7259 Aus: Vic ITS 28S 0 0
L canaliculata GMM7266 Aus: Vic ITS 28S RPB2 0
L canaliculata GMM7206 Aus: Vic ITS 28S RPB2 0
L fibrillosa GMM7562 NZ: Southland ITS 28S RPB2 0
Lycoperdon pyriforme AFTOL480 AY854075 AF287873 AY218495 AY883426
L caniculata GMM7267 JX504137 JX504213 RPB2 EF1a
L masoni GMM7413 Aus: Tas ITS 28S RPB2 EF1a
L sp KH_NZ06_135 NZ: TeToto ITS 28S RPB2 EF1a
H carneum Trappe27608 Aus: Vic ITS 28S RPB2 0
L sp GMM7470 Aus: Tas ITS 28S RPB2 EF1a
L canaliculata GMM7260 Aus: Vic ITS 28S 0 0
L masonii GMM7488 NZ: Nelson ITS 0 0 0
Cortinarius iodes AFTOL285 AF389133 AY702013 AY536285 AY881027
L sp KH_NZ06_034 NZ: Thames ITS 28S 0 0
L ohiensis GMM7593 NZ: Canterbury ITS 28S RPB2 EF1a
H sp Trappe31032 Aus: Vic ITS 0 RPB2 0
L lateritia GMM7447 Aus: Tas ITS 28S RPB2 EF1a
L sp GMM7451 Aus: Tas ITS 28S RPB2 0
L sp KH_NZ06_137 NZ: Hakarimata ITS 28S RPB2 EF1a
L Lat1a GMM7258 Aus: Vic ITS 28S RPB2 0
L sp2 GMM7284 PNG ITS 28S RPB2 EF1a
L sp GMM7400 Aus: Qland ITS 28S RPB2 EF1a
L ohiensis GMM7551 NZ: Southland ITS 28S RPB2 0
L ohiensis GMM7564 NZ: Southland ITS 28S RPB2 0
L canaliculata GMM7209 Aus: Vic JX504136 JX504212 RPB2 0
L sp GMM7398 Aus: Qland ITS 28S RPB2 EF1a
L ohiensis GMM7539 NZ: Southland ITS 28S RPB2 EF1a
L masonii GMM7501 NZ: Buller ITS 28S RPB2 0
L sp1 GMM7280 PNG ITS 28S RPB2 EF1a
L NZ9 GMM7522 NZ: Otago ITS 28S RPB2 0
L sp KH_NZ06_064 NZ: Moturere ITS 28S RPB2 EF1a
H NZhydnan GMM7584NZ NZ: Canterbury ITS 28S RPB2 EF1a
L tetraspora F1080957 Argentina: Tierra del Fuego ITS 28S 0 0
H carneum Trappe31123 Aus: Capital ITS 28S RPB2 EF1a
2
3
4
5
6
7
8
9
1
10
11
83/0.99
98
100/1.0
100/
1.0
97/1.0
85/1.0
90/
1.0
87/1.0
97/
1.0
88/1.0
80
87
96/1.0
87
79
86
99
100/1.0
93
89/
1.0
90
81
100/1.0
86/1.0
99/1.0
97/
1.0
100/1.0
100/
1.0
100/1.0
100
99/1.0
82
91/1.0
98/
1.0
100/
1.0
85/
1.0
94/
0.99
100/1.0
100/1.0
79/
0.99
96/1.0
95/
1.0
88/1.0
96
100/1.0
94/1.0
90/
0.99
100/1.0
100/
1.0
100/
1.0
99/1.0
88/1.0
100/1.0
91/1.0
100/1.0
97/0.99
71
85/
0.99
89
85/1.0
100/1.0
97
100/1.0
99
76/1.0
100
90/1.0
81/0.99
93
0.99
0.99
0.96
A
B
C
D
E
L
M
N
O
P
Laccaria BASAL
Kentaro Hosaka
Morphologically L. BASAL has all the
features of a good Laccaria taxon.
Morphological description in Note S8.
Laccaria sp A
G.M. Muller
Hydnangium
carneum
G.M. Muller

Notes S1b
Global Laccaria Phylogeny
Bayesian Relaxed Clock Ultrametric Tree
4 genes: ITS, 28S, RPB2, EF1a
(Sample IDs are indicated on the branch labels.
Refer to Notes S2 for GenBank accession numbers.)
ntaxa=117 (116 ingroup)
QPliMioceneOligoceneEocenePaleoEPOCH late Cretaceous
0.0 Ma
10.0
20.0
30.0
40.0
50.0
60.0
70.0
80.0
90.0
QF
FP
Q
S
Q
Q
L
Q
P
DP
D
DP
Cs Li
Li
Al
E
N
N
K
N
N
N
N
N
E
Ac
Le
P
M
E
Ac
K
Le
N
E
EN
Ac
N
N
N
Le
EN
E
E
E
E
E
E
E
E
E
E
N
N
N
N
N
N
N
N
N
N
Le
Le
N
Ac
K
Le
Le
Al
Le
Cs
D
Q
Pi
A
A
Ps
F
F
F
A
Pi
L
P
Cs
Cs
Cn
Q
A
Cn
MIX
Q
P
P
Cs
P
A
S
Pi
AP
Pi
AP
Pi
AP
Pi
N
N
E
Ac
Q
Q
P
Q
F
A
P
Cn
Q
A
F
MIX
MIX
Pi
Cs
5.95
20.49
13.07
6.16
8.03
12.02
15.05
4.04
7.19
23.91
5.73
11.97
17.87
9.58
31.17
9.29
10.28
13.07
11.13
4.62
8.77
4.13
15.25
9.69
9.04
13.04
12.83
22.16
13.14
6.68
35.98
21.51
2.96
3.38
4.63
13.76
5.63
15.68
4.3
64.2
7.48
21.27
2.56
13.73
8.48
9.99
16.81
10.99
42.49
3.66
6.69
9.72
12.06
10.32
2.19
10.69
5.09
7.83
10.88
23.78
24.05
14.71
4.41
3.83
3.76
3.86
6.37
3.93
9.56
23.69
18.55
13.64
14.5
26.61
4.79
12.19
14.05
7.0
10.14
79.53
29.29
11.79
28.34
2.71
4.83
19.59
15.44
2.88
14.37
17.04
21.24
17.61
7.9
6.15
21.79
11.78
1.94
17.68
11.79
16.08
10.37
16.0
5.64
27.97
10.12
9.86
12.62
8.98
16.99
6.86
A
B
C
D
F
E
G
H
I
J
K
L
M
N
O
P
Branch Support Statistics
Above branch = Maximum Likelihood Bootstrap %
Below branch = Bayesian Posterior Probabilities

90% - 80% bootstrap or 0.98 - 0.95 Posterior Probability
p=0.901
p=0.098
p=0.05
p=0.096
p=0.091
L NZ5 GMM7521 - NEW ZEALAND
L pseudomontana Cripps1625 - USA
L NZ6 GMM7564 - NEW ZEALAND
L laccata SB2135 - PORTUGAL
L ambigua sp. nov. PDD89696 - NEW ZEALAND
L amethysteo-occidentalis AWW556 - USA
L MAS1 GMM7200 - AUSTRALIA
L sp KH-LA06-002 - USA
L NZ4 GMM7551 - NEW ZEALAND
L aurantia GMM6172 - CHINA
L galerenoides F1080983 - ARGENTINA
L ohiensis KH-07192006-1 - USA
L aff nobilis AWW584 - USA
L LAT1a GMM7258 - AUSTRALIA
L fibrillosa s.l. GMM7533 - NEW ZEALAND
L bicolor S238N - USA
L bullipellis AWW465 - CHINA
L sp AWW569 - USA
L sp GMM6094 - CHINA
L sp TWO1184 - THAILAND
L sp AWW572 - USA
L sp TWO1178 - THAILAND
L NZ9 GMM7522 - NEW ZEALAND
L SPD GMM7217 - AUSTRALIA
L masonii s.l. GMM7501 - NEW ZEALAND
L himalayensis AWW463 - CHINA
L masonii s.l. GMM7488 - NEW ZEALAND
L NZ3 KH-NZ06-137 - NEW ZEALAND
L NZ2 KH-NZ06-135 - NEW ZEALAND
H sp GMM7584NZ - NEW ZEALAND
L sp AWW567 - USA
L vinaceoavellanea A2986 - JAPAN
L SPA GMM7422 - AUSTRALIA
L PNG1 GMM7280 - PAPUA NEW GUINEA
L B2 MEL2359670
L ARG2 A2064 - ARGENTINA
L aff bicolor HKAS44062 - CHINA
L aff montana GMM7630 - CHINA
L NZ8 KH-NZ06-119 - NEW ZEALAND
L acanthospora AWW485 - CHINA
L Q2 GMM7411 - AUSTRALIA
L B3 MEL226520
L laccata SB2067 - PORTUGAL
L laccata var pallidifolia GMM7605 - FRANCE
L Q1 GMM7403 - AUSTRALIA
L sp GMM7451 - AUSTRALIA
L sp TWO1194 - THAILAND
L sp GMM6585 - COSTA RICA
L aff alba HKAS57950 - CHINA
L aff ohiensis AWW545 - USA
L SPC2 KH-NZ06-064 - NEW ZEALAND
H sublamellatum MEL2297141 - AUSTRALIA
L sp GMM7580 - NEW ZEALAND
L sp AB211271 - JAPAN
H sp Trappe31032 - AUSTRALIA
L calospora F1104722 - COSTA RICA
L sp GMM5935
L LAT2 GMM7276 - AUSTRALIA
L ARG1 F1080957 - ARGENTINA
L amethystina GMM7621 - FRANCE
L CAN1 GMM7227 - AUSTRALIA
Mythicomyces corneipes AFTOL 972
H HCAR2 MEL2359676
L sp DED7426 - THAILAND
L CAN2 MEL2359684
L sp GMM6639 - CHINA
L sp GMM7266 - AUSTRALIA
L Lat3 GMM7207 - AUSTRALIA
L cf laccata A2987 - JAPAN
L vinaceobrunea KH-LA06-018 - USA
L sp GMM7613 - FRANCE
L PNG5 OSC122484 - PAPUA NEW GUINEA
L sp F1102432 - COSTA RICA
L negrimarginata GMM7631Tibet - CHINA
L CAN5 GMM7205 - AUSTRALIA
L CAN3 MEL2359685
L CAN6 GMM7413 - AUSTRALIA
L ohiensis GMM7028 - RUSSIA
L violaceoniger s.l. GMM7508 - NEW ZEALAND
L PNG4 GMM7316 - PAPUA NEW GUINEA
L sp A0584 - JAPAN
L trichodermophora F1111951 - COSTA RICA
L sp GMM6723 - CHINA
L sp GMM6800
L laccata SB2133 - PORTUGAL
L longipes F1092175 - USA
H HCAR4 Trappe14544 - AUSTRALIA
L major GMM6019 - COSTA RICA
L CAN4 MEL2359686 - AUSTRALIA
L sp GMM7733 - USA
L SPB1 GMM7447 - AUSTRALIA
L sp GMM6776 - CHINA
L proxima GMM7584 - RUSSIA
L sp TWO1168 - THAILAND
L SPS Syme1586-06 - AUSTRALIA
L NZ10 GMM7539 - NEW ZEALAND
L alba AWW438 - CHINA
L trullisata PRL7587 - USA
L sp ALB183 - CHINA
L ochropurpurea PRL3777 - USA
L laccata s.l. GMM7586 - RUSSIA
L fulvogrisea GMM6623 - CHINA
L sp F1068333 - USA
L sp GMM7596 - CHINA
L PNG3 GMM7302 - PAPUA NEW GUINEA
L sp SB2238 - PORTUGAL
L masonii s.l. GMM7473 - NEW ZEALAND
L sp GMM7265 - AUSTRALIA
L pumila GMM7637 - FRANCE
L sp DED7419 - THAILAND
L lilacina GMM7531 - NEW ZEALAND
L sp A0553 - JAPAN
L MAS2 GMM7416 - AUSTRALIA
L E1 GMM7440 - AUSTRALIA
L PNG2 GMM7282 - PAPUA NEW GUINEA
L LAT1 GMM7251 - AUSTRALIA
H HCAR1 GMM7273lepto - AUSTRALIA
Host Associations
Quercus
Fagus
Castanea
Castanopsis
Lithocarpus
Allocasuarina
Nothofagus
Eucalyptus
Kunzea
Melaleuca
Leptospermum
Salix
Dipterocarpus
Acacia
Betula
Q
F
Cn
N
D
E
Ac
Le
P
L
S
Li
Al
K
M
Pi
Ps
Abies
Pinus
Larix
Picea
Pseudotsuga
A
Cs
B
Angiosperms
Gymnosperms

Notes S2 – Laccaria specimen and dataset information Wilson et al. Laccaria evolution
S2 page 1
Table S2a Laccaria specimen IDs, geography, species assignment, and GenBank accession numbers.
Genus species (when
feasable)
Specimen-
voucher
country
Assigned species/complex
ITS
28S
RPB2
EF1a
Laccaria sp.
A0051
Japan: Narusawa
JP4
KU685613
–
–
–
Laccaria sp.
A0087
Japan: Fujikawaguchiko
Laccaria amythestina complex
KU685614
–
–
–
Laccaria violaceoniger
A0492
New Zealand
NZ1
KU685615
–
–
–
Laccaria cf. laccata
A0553
Japan: Makomanai
JP1
JN942780
JN939733
JN993511
–
Laccaria sp.
A0556
Japan: Makomanai
JP1
KU685616
–
–
–
Laccaria sp.
A0561
Japan: Sapporo
Laccaria alba complex
JX504082
–
–
–
Laccaria sp.
A0573
Japan: Narusawa
Laccaria alba complex
KU685617
–
–
–
Laccaria sp.
A0576
Japan: Tsuchiyu
JP1
KU685618
–
–
–
Laccaria sp.
A0577
Japan: Iwamizawa
JP1
KU685619
–
–
–
Laccaria sp.
A0584
Japan: Makomanai
JP2
KU685620
–
–
–
Laccaria sp.
A0585
Japan: Makomanai
JP2
KU685621
–
–
–
Laccaria sp.
A1800
Taiwan
KU685622
–
–
–
Laccaria sp.
A2062
Argentina
Arg2
KU685623
–
–
–
Laccaria sp.
A2064
Argentina
Arg2
KU685624
–
–
–
Laccaria galerinoides
A2103
Argentina
KU685625
–
–
–
Laccaria sp.
A2111
Argentina
Arg1
KU685626
–
–
–
Laccaria vinaceoavellanea
A2986
Japan: Fushimi
JN942810
JN939738
JN993520
–
Laccaria sp.
A2987
Japan: Fushimi
JP1
JN942786
JN939739
JN993521
–
Laccaria sp.
A3344
China
Laccaria amythestina complex
KU685627
–
–
–
Laccaria cf laccata
A3394
Japan: Suzuka
JP1
JN942788
JN939770
JN993522
–
Laccaria ochropurpurea
AFTOL447
–
AY700200
DQ472731
–
Laccaria alba
AWW438
China: Yunnan Shangrila
Laccaria alba complex
JX504094
JX504178
KU685912
KU686072
Laccaria himalayensis
AWW463
China: Tibet, Linzhi
JX504098
JX504182
KU685913
–
Laccaria bullipellis
AWW465
China: Tibet, Linzhi
JX504100
JX504184
KU685914
–
Laccaria himalayensis
AWW484
China: Tibet, Linzhi
JX504101
JX504185
KU685915
–
Laccaria acanthospora
AWW485
China: Tibet, Linzhi
JX504102
JX504186
KU685916
KU686073
Laccaria aff bicolor
AWW539
USA: Illinois
Laccaria bicolor complex
KM067817
KU685763
–
–
Laccaria tortillis
AWW545
USA: Illinois
JX504106
JX504190
KU685917
–
Laccaria cf laccatta
AWW555
USA: California, Nevada
Co.
Laccaria laccata complex
–
KU685764
KU685918
KU686074
Laccaria amethysteo-
occidentalis
AWW556
USA: California, Nevada Co.
JX504107
JX504191
KU685919
–
Laccaria sp.
AWW567
USA: Michigan, U.P.
Laccaria bicolor complex
KM067824
KU685765
–
–

Notes S2 – Laccaria specimen and dataset information Wilson et al. Laccaria evolution
S2 page 2
Laccaria sp.
AWW569
USA: Michigan, U.P.
JX504108
KU685766
KU685920
–
Laccaria sp.
AWW572
USA: Michigan, U.P.
JP4
KM067827
KU685767
KU685921
–
Laccaria sp.
AWW583
USA: Oregon, Benton Co.
Laccaria laccata complex
KM067828
KU685768
–
–
Laccaria nobilis
AWW584
USA: Oregon, Benton Co.
JX504110
JX504193
KU685922
–
Laccaria amethysteo-
occidentalis
AWW590
USA: Oregon, Benton Co.
JX504112
JX504195
KU685923
–
Laccaria sp.
AWW591
USA: Oregon, Benton Co.
Laccaria bicolor complex
–
KU685769
KU685924
–
Laccaria sp.
AWW596
USA: AK
pan-beringia bicolor
JX504116
JX504199
–
–
Laccaria negrimarginata
BAP360
China: Tibet
JX504120
–
–
–
Laccaria sp.
DED7419
Thailand
THAI2
KX513839
KX513840
KU685770
KU685925
KU686075
Laccaria sp.
DED7426
Thailand
THAI1
KU685628
KU685771
KU685926
KU686076
Laccaria sp.
DED7435
Thailand
THAI2
KX513841
KU685772
KU686060
–
Hydnangium vinaceoavellanea
F1061267
Japan//Tottori
JX504121
–
–
–
Laccaria sp.
F1068333
USA: Wisconsin
L. bicolor complex
KU685629
KU685773
KU686061
–
Laccaria bicolor
GMM2692
Chile, Maullin
F1077767; proxima 1
KU685630
KU685774
–
–
Laccaria tetraspora
F1080957
Argentina:
Tierra/del/Fuego
Arg1
KU685631
KU685775
–
–
Laccaria galerinoides
F1080983
Argentina
KU685632
KU685776
KU685927
KU686077
Laccaria proximella
F1081079
Argentina: Neuquen
proxima 1
KU685633
KU685777
KU685928
–
Laccaria galerinoides
F1081213
Chile
KU685634
KU685778
KU685929
KU686078
Laccaria bicolor
GMM2118
USA: California,
Mendocino
F1091205
KU685635
–
–
–
Laccaria nobilis
F1091206
USA: Michigan
L. bicolor complex; "nobilis"
KU685636
KU685779
–
–
Laccaria longipes
F1092175
USA: Michigan
KU685637
KU685780
–
–
Laccaria sp.
F1102432
Costa Rica
L. aff amythestina 2
KU685638
KU685781
KU686062
–
Laccaria gomezii
F1104722
Costa Rica
KU685639
KU685782
–
–
Laccaria vinaceobrunnea
F1110429
USA: Texas
–
KU685783
–
–
Laccaria sp.
F1111951
Costa Rica
L. "trichodermophora"
KU685640
KU685784
KU686063
–
Laccaria tortilis
F1116205
USA: Illinois
KU685641
KU685785
–
–
Laccaria sp.
F1120731
China
JP1
JX504125
JX504205
–
–
Laccaria alba
F1120750
China
L. alba complex
JX504126
JX504242
–
–
Laccaria bicolor
F1121424
China
JX504127
JX504207
KU686064
–
Laccaria proxima
F1133825
USA: Mississippi
L. bicolor complex
KU685642
KU685786
KU686065
–
Laccaria sp.
GMM5935
New Zealand
KU685643
KU685787
KU686066
–
Laccaria gomezii
GMM6022
Costa Rica
KU685644
–
–
–

Notes S2 – Laccaria specimen and dataset information Wilson et al. Laccaria evolution
S2 page 3
Laccaria bicolor
GMM6094
China: Jilin Province
KM067831
KU685788
KU686067
–
Laccaria alba
GMM6131
China: Chang Bai Shan
JX504131
JX504210
KU685930
KU686079
Laccaria aurantia
GMM6172
China: Yunnan
KU685645
KU685789
KU685931
–
Laccaria fulvogrisea
GMM6623
China
KU685646
KU685790
KU685932
KU686080
Laccaria sp.
GMM6585
Costa Rica
KU685647
KU685791
KU685933
–
Laccaria sp.
GMM6595
China: Yunnan
CHINA1
KU685648
–
KU685934
–
Laccaria sp.
GMM6679
China: Yunnan
CHINA1
KU685649
KU685792
KU685935
KU686081
Laccaria sp.
GMM6723
China: Yunnan
N25° 40.566' E 98° 42.166'
KU685650
KU685793
KU685936
–
Laccaria sp.
GMM6776
China: Yunnan
CHINA2
KU685651
KU685794
KU685937
KU686082
Laccaria sp.
GMM7020
Russia: Caucasus
"laccata"
KU685652
KU685795
KU685938
–
Laccaria sp.
GMM7028
Russia: Caucasus
L. macrocystidia complex
KU685653
KU685796
KU685939
–
Laccaria amethystina
GMM7041
Russia: Caucasus
KU685654
KU685797
KU685940
–
Laccaria sp.
GMM7167
Costa Rica
L. aff amythestina 2
KU685655
KU685798
–
KU686083
Laccaria masonii
GMM7200
Australia: Victoria
MAS1
KU685656
KU685799
KU685941
KU686084
Laccaria canaliculata
GMM7205
Australia: Victoria
CAN5
KU685657
KU685800
KU685942
KU686085
Laccaria canaliculata
GMM7206
Australia: Victoria
CAN5
KU685658
KU685801
KU685943
–
Laccaria canaliculata
GMM7209
Australia: Victoria
CAN1
JX504136
JX504212
KU685944
–
Laccaria sp.
GMM7212
Australia: Victoria
sp.C
KU685659
KU685802
KU685945
–
Laccaria sp.
GMM7213
Australia: Victoria
sp.E
KU685660
KU685803
KU685946
KU686086
Laccaria sp.
GMM7217
Australia: Victoria
sp.D
KU685661
KU685804
KU685947
KU686087
Laccaria lateritia
GMM7220
Australia: Victoria
LAT2
KU685662
KU685805
KU685948
–
Laccaria lateritia
GMM7221
Australia: Victoria
LAT1
KU685663
KU685806
KU685949
–
Laccaria canaliculata
GMM7222
Australia: Victoria
LAT1
KU685664
KU685807
KU685950
–
Laccaria sp.
GMM7226
Australia: Victoria
sp.A
KU685665
KU685808
KU685951
KU686088
Laccaria canaliculata
GMM7227
Australia: Victoria
CAN1
KU685666
KU685809
KU685952
KU686089
Laccaria sp.
GMM7240
Australia: Victoria
sp.D
KU685667
KU685810
KU685953
–
Laccaria lateritia
GMM7250
Australia: Victoria
LAT2
KU685668
KU685811
KU685954
–
Laccaria canaliculata
GMM7251
Australia: Victoria
LAT1
KU685669
KU685812
KU685955
KU686090
Laccaria sp.
GMM7254
Australia: Victoria
sp.B1
KU685670
KU685813
–
–
Laccaria sp.
GMM7257
Australia: Victoria
sp.C2
KU685671
KU685814
KU685956
KU686091
Laccaria sp.
GMM7259
Australia: Victoria
sp.B1
KU685672
KU685815
–
–
Laccaria canaliculata
GMM7260
Australia: Victoria
LAT1
KU685673
KU685816
–
–
Laccaria canaliculata
GMM7264
Australia: Victoria
LAT1
KU685674
KU685817
KU685957
–
Laccaria sp.
GMM7265
Australia: Victoria
sp.B5
KU685675
KU685818
KU685958
KU686092

Notes S2 – Laccaria specimen and dataset information Wilson et al. Laccaria evolution
S2 page 4
Laccaria sp.
GMM7266
Australia: Victoria
"canaliculata"
KU685676
KU685819
KU685959
–
Laccaria caniculata
GMM7267
Australia: Victoria
LAT1
JX504137
JX504213
KU685960
KU686093
Hydnangium carneum
GMM7272
Australia: Victoria
HCAR1
KU685677
KU685820
KU685961
KU686094
Hydnangium carneum
GMM7273lepto.
Australia: Victoria
HCAR1
KU685678
KU685821
KU685962
KU686095
Laccaria sp.
GMM7274
Australia: Victoria
sp.E1
KU685679
KU685822
KU685963
KU686096
Laccaria canaliculata
GMM7276
Australia: Victoria
LAT2
KU685680
KU685823
KU685964
KU686097
Laccaria PNG1
GMM7280
Papua New Guinea
PNG1
KU685681
KU685824
KU685965
KU686098
Laccaria PNG2
GMM7282
Papua New Guinea
PNG2
KU685682
KU685825
KU685966
KU686099
Laccaria PNG2
GMM7284
Papua New Guinea
PNG2
KU685683
KU685826
KU685967
KU686100
Laccaria PNG1
GMM7285
Papua New Guinea
PNG1
KU685684
KU685827
KU685968
–
Laccaria PNG3
GMM7301
Papua New Guinea
PNG3
–
KU685828
KU685969
KU686101
Laccaria sp.
GMM7302
Papua New Guinea
PNG3
JX504138
JX504214
KU685970
KU686102
Laccaria PNG4
GMM7316
Papua New Guinea
PNG4
KU685685
KU685829
KU685971
KU686103
Laccaria sp.
GMM7398
Australia: Queensland
Q2
KU685686
KU685830
KU685972
KU686104
Laccaria sp.
GMM7400
Australia: Queensland
Q2
KU685687
KU685831
KU685973
KU686105
Laccaria sp.
GMM7402
Australia: Queensland
Q1
KU685688
KU685832
KU685974
KU686106
Laccaria sp.
GMM7403
Australia: Queensland
Q1
KU685689
KU685833
KU685975
KU686107
Laccaria laccata
GMM7411
Australia: Queensland
Q2
KU685690
KU685834
KU685976
KU686108
Laccaria masoni
GMM7413
Australia: Tasmania
CAN1
KU685691
KU685835
KU685977
KU686109
Laccaria masoni
GMM7416
Australia: Tasmania
MAS2
KU685692
KU685836
KU685978
KU686110
Laccaria masoni
GMM7420
Australia: Tasmania
MAS1
KU685693
KU685837
KU685979
–
Laccaria sp.A
GMM7422
Australia: Tasmania
sp.A
KU685694
KU685838
KU685980
KU686111
Laccaria sp.E
GMM7440
Australia: Tasmania
sp.E
KU685695
KU685839
KU685981
KU686112
Laccaria lateritia
GMM7447
Australia: Tasmania
sp.B1
KU685696
KU685840
KU685982
KU686113
Hydnangium carneum
GMM7449
Australia: Tasmania
HCAR1
KU685697
KU685841
KU685983
KU686114
Laccaria sp.
GMM7451
Australia: Tasmania
KU685698
KU685842
KU685984
–
Laccaria sp.E
GMM7454
Australia: Tasmania
sp.E
KU685699
–
–
–
Laccaria sp.A
GMM7457
Australia: Tasmania
sp.A
KU685700
KU685843
KU685985
–
Laccaria canaliculata
GMM7458
Australia: Tasmania
CAN4
KU685701
–
–
–
Laccaria sp.
GMM7470
Australia: Tasmania
sp.B1
KU685702
KU685844
KU685986
KU686115
Laccaria masonii
GMM7473
New Zealand: Buller
NZ1complex
KU685703
KU685845
KU685987
KU686116
Laccaria masonii
GMM7488
New Zealand: Nelson
masonii/fibrillosa/lilacina
complex
KU685704
–
–
–
Laccaria masonii
GMM7501
New Zealand: Buller
masonii/fibrillosa/lilacina
complex
KU685705
KU685846
KU685988
–

Notes S2 – Laccaria specimen and dataset information Wilson et al. Laccaria evolution
S2 page 5
Laccaria fibrillosa
GMM7508
New Zealand: Karamea
NZ1complex
KU685706
KU685847
KU685989
–
Laccaria violaceoniger
GMM7520
New Zealand: Otago
NZ1complex
KU685707
KU685848
KU685990
–
Laccaria glabripies
GMM7521
New Zealand: Otago
KU685708
KU685849
KU685991
KU686117
Laccaria lilacina
GMM7531
New Zealand: Otago
masonii/fibrillosa/lilacina
complex
KU685709
KU685850
KU685992
KU686118
Laccaria violaceoniger
GMM7533
New Zealand: Otago
NZ1complex
KU685710
KU685851
KU685993
–
Laccaria glabripes
GMM7534
New Zealand: Otago
KU685711
KU685852
–
–
Laccaria ohiensis
GMM7539
New Zealand: Southland
NZ10
KU685712
KU685853
KU685994
KU686119
Laccaria ohiensis
GMM7551
New Zealand: Southland
NZ4
KU685713
KU685854
KU685995
–
Laccaria fibrillosa
GMM7562
New Zealand: Southland
masonii/fibrillosa/lilacina
complex
KU685714
KU685855
KU685996
–
Laccaria ohiensis
GMM7564
New Zealand: Southland
NZ6
KU685715
KU685856
KU685997
–
Laccaria violaceoniger
GMM7580
New Zealand: Southland
NZ1complex
KU685716
KU685857
KU685998
–
Laccaria proxima
GMM7584
Russia: Komorav
proxima 1
KU685717
KU685858
KU685999
KU686120
Laccaria laccata
GMM7586
Russia: Komorav
Russia-China group?
KM067835
KU685859
KU686000
–
Laccaria ohiensis
GMM7493NZ
New Zealand: Canterbury
NZ3
KU685718
KU685860
KU686001
KU686121
Laccaria macrocystidia
GMM7612
France: Vallon
KM067847
KU685861
KU686002
KU686122
Laccaria macrocystidia
GMM7613
France: Vallon
KM067848
KU685862
KU686003
KU686123
Laccaria macrocystidia
GMM7616
France: Vallon
KM067850
KU685863
KU686004
–
Laccaria sp.
GMM7625
France: Forest comaniale
"laccata"
–
KU685864
KU686005
KU686124
Laccaria macrocystidia
GMM7626
France: Forest comaniale
KM067856
KU685865
KU686006
KU686125
Laccaria sp.
GMM7627
France: Forest comaniale
"laccata"
–
KU685866
KU686007
KU686126
Laccaria macrocystidia
GMM7628
France: Forest comaniale
KM067857
KU685867
KU686008
KU686127
Laccaria sp.
GMM7630tibet
China: Tibet
montana/pumula complex
JX504151
JX504225
KU686009
KU686128
Laccaria bicolor/proxima
GMM7631France
France: Forest comaniale
proxima 1
KM067858
KU685869
KU686010
KU686129
Laccaria negrimarginata
GMM7631Tibet
China: Tibet
JX504152
JX504226
KU686011
KU686130
Laccaria trichodermophora
GMM7712
USA: Texas, Newton Co.
L. bicolor complex
KM067866
–
KU686012
–
Laccaria trichodermophora
GMM7733
USA: Texas, Tyler Co.
L. "trichodermophora"
JX504157
JX504230
KU686013
–
Laccaria alba
HKAS41457
China: Yunnan Longling
–
JX504232
–
–
Laccaria angustilamella
HKAS41483
China: Yunnan Longling
–
JX504233
–
–
Laccaria sp.
HKAS42577
China: Yunnan
specimen named "alba"
–
JX504234
–
–
Laccaria sp.
HKAS44062
China: Yunnan
pan-beringia bicolor
JX504159
JX504235
KU686068
–
Laccaria acanthospora
HKAS45998
China: Tibet
KU685719
KU685870
KU686069
–
Laccaria sp.
HKAS57950
China: Tibet
L. alba complex
JX504166
JX504242
–
–
Laccaria ohiensis
KH_07192006_1
USA: Indiana
KU685720
KU685871
KU686014
–

Notes S2 – Laccaria specimen and dataset information Wilson et al. Laccaria evolution
S2 page 6
Laccaria trichodermophora
KH_LA06_013
USA: Louisiana
L. "trichodermophora"
KM067881
KU685872
–
–
Laccaria ochropurpurea
KH_LA06_016
USA: Louisiana
KU685721
–
–
–
Laccaria vinaceobrunea
KH_LA06_018
USA: Louisiana
–
KU685873
KU686015
–
Laccaria sp.
PDD 89649
New Zealand: Thames
NZ4
KU685722
KU685874
–
–
Laccaria sp.
PDD 89677
New Zealand: Moturere
sp.C2
KU685723
KU685875
KU686016
KU686131
Laccaria sp.
PDD 89685
New Zealand: Coromandel
NZ4
KU685724
–
KU686017
–
Laccaria ambigua
PDD 89696
New Zealand: Kauri Grove
L ambigua
KU685725
KU685876
KU686018
KU686132
Laccaria sp.
PDD 89737
New Zealand: TeToto
NZ8
KU685726
KU685877
KU686019
KU686133
Laccaria sp.
PDD 89739
New Zealand: TeToto
NZ3
KU685727
KU685878
–
KU686134
Laccaria sp.
PDD 89750
New Zealand: TeToto
NZ2
KU685728
KU685879
KU686020
KU686135
Laccaria sp.
PDD 89752
New Zealand: Hakarimata
NZ3
KU685729
KU685880
KU686021
KU686136
Laccaria sp.
PDD 89812
New Zealand: Waitakere
NZ2
KU685730
KU685881
KU686022
KU686137
Laccaria amethystina
LaAM-08-1
JGI/Genome
JGI
JGI
JGI
JGI
Hydnangium sublamellatum
MEL2297141
Australia: Western
Australia
KU685731
KU685882
KU686023
KU686138
Laccaria sp.
MEL2359686
Australia: Tasmania
CAN4
JX270723
–
–
JX305622
Laccaria sp.
MEL2359670
B2
JX270726
–
JX295596
JX305610
Laccaria sp.
MEL226520
B3
JX270713
–
JX295628
JX305639
Laccaria sp.
MEL2359685
CAN3
JX270697
–
JX295600
JX305613
Hydnangium HCAR2
MEL2359676
HCAR2
JX270733
–
–
JX305633
Laccaria sp.
MEL2359684
CAN2
JX270714
–
JX295587
JX305600
Laccaria ochropurpurea
PRL3777
USA: Illinois
KU685732
JX504246
KU686024
–
Laccaria ochropurpurea
PRL4777
USA: Illinois
KU685733
KU685883
KU686025
–
Laccaria bicolor
S238N
JGI/Genome
DQ179123
–
JGI
JGI
Laccaria sp.
SB2067
Portugal
L. amythestina complex
KM067885
JX504248
KU686026
–
Laccaria sp.
SB2133
Portugal
PORT1
KM067887
KU685884
KU686027
KU686139
Laccaria sp.
SB2135
Portugal
"laccata"
JX504172
JX504249
KU686028
KU686140
Laccaria sp.
SB2210
Portugal
PORT1
KM067890
KU685885
KU686029
KU686141
Laccaria bicolor
SB2238
Portugal
PORT2
KM067892
KU685886
KU686030
–
Laccaria bicolor
SB2239
Portugal
PORT2
KM067893
KU685887
KU686031
–
Laccaria sp.
Syme1586/06
Australia: Western
Australia
sp.S
KU685734
KU685888
KU686032
KU686142
Hydnangium sp.
Trappe14544
Australia: Tasmania
CAR4
KU685735
KU685889
KU686033
–
Hydnangium carneum
Trappe27608
Australia: Victoria
HCAR1
KU685736
KU685890
KU686034
–
Hydnangium carneum
Trappe27650
Australia: New South
Wales
HCAR1
KU685737
KU685891
KU686035
–

Notes S2 – Laccaria specimen and dataset information Wilson et al. Laccaria evolution
S2 page 7
Hydnangium carneum
Trappe27676
Australia: Victoria
HCAR1
KU685738
–
–
–
Hydnangium sp.
Trappe31032
Australia: Victoria
KU685739
–
KU686036
–
Hydnangium sp.
Trappe31056
Australia: Victoria
HCAR1
KU685740
–
KU686037
KU686143
Hydnangium carneum
Trappe31123
Australia: Capital
HCAR1
KU685741
KU685892
KU686038
KU686144
Hydnangium carneum
Trappe31270
Australia: New South
Wales
HCAR1
KU685742
KU685893
KU686039
KU686145
Laccaria sp.
TWO1150
Thailand
THAI3
KU685743
KU685894
KU686040
–
Laccaria sp.
TWO1166
Thailand: Chaing Mai
THAI1
KU685744
KU685895
KU686041
–
Laccaria sp.
TWO1168
Thailand: Chaing Mai
THAI3
KU685745
KU685896
KU686042
KU686146
Laccaria sp.
TWO1178
Thailand: Chaing Mai
THAI4
KU685746
KU685897
KU686043
KU686147
Laccaria sp.
TWO1184
Thailand: Chaing Mai
THAI6
KU685747
KU685898
–
KU686148
Laccaria sp.
TWO1194
Thailand: Prajenburi
THAI5
KU685748
KU685899
–
KU686149
Laccaria trullisata
WCG2072
KU685749
–
KU686044
KU686150
Laccaria sp.
ZT9196
Indonesia: Lombok
THAI4
KU685750
KU685900
KU686070
–
Laccaria salmonicolor
GMM7596tibet
China: Tibet
JX504143
JX504218
KU686045
KU686151
Laccaria amethystina
GMM7621
France: Forest comaniale
JX504150
JX504224
KU686046
KU686152
Laccaria trullisata
PRL7587
KM067882
JX504247
KU686047
KU686153
Laccaria laccata var. pallidifolia
GMM7605
France: Vallon
KM067844
KU685901
KU686048
KU686154
Laccaria sp.
GMM7258
Australia: Victoria
Lat1a
KU685751
KU685902
KU686049
–
Laccaria sp.
GMM7207
Australia: Victoria
Lat3
KU685752
KU685903
KU686050
–
Laccaria sp.
GMM7522
New Zealand: Otago
NZ9
KU685753
KU685904
KU686051
–
Hydnangium sp.
GMM7484NZ
New Zealand: Canterbury
NZhydnan
KU685754
KU685905
KU686052
KU686155
Laccaria sp.
OSC122484
Papua New Guinea
PNG5
KU685755
–
–
–
Laccaria pumila
AF287869
–
AF287869
–
–
Laccaria tortilis
GMM7635
France: Forest comaniale
KM067859
KU685906
KU686053
KU686156
Laccaria aff. montana
AWW446
China: Tibet
montana/pumula complex
JX504097
JX504181
KU686054
KU686157
Laccaria pumila
GMM7637
France: Fraychirede Bog
montana/pumula complex
KM067861
JX504229
–
KU686158
Laccaria pseudomontana
Cripps1625
USA: Colorado
DQ149871
–
–
–
Laccaria pseudomontana
Cripps1771
USA: Colorado
DQ149870
–
–
–
Laccaria sp.
GMM6800
Guatemala
KU685756
KU685907
KU686055
KU686159
Laccaria major
GMM6019
Costa Rica
KU685757
KU685908
KU686056
KU686160
Laccaria major
GMM6012
Costa Rica
KU685758
KU685909
KU686057
–
Laccaria sp.
ALB183
China: Tibet
Laccaria aff amythestina 1
JX504092
JX504176
KU686058
KU686161
Laccaria amethystina
KH_LA06_002
USA: Louisiana
KU685759
KU685910
KU686059
KU686162
Laccaria sp.
F1123822
USA: Wisconsin
Laccaria aff amythestina 1
KU685760
KU685911
KU686071
–
Laccaria amethystina
AB211270
Japan
AB211270
–
–
–
Laccaria amethystina
AB211271
Japan
AB211271
–
–
–

Notes S2 – Laccaria specimen and dataset information Wilson et al. Laccaria evolution
S2 page 8
Laccaria sp.
A4296
New Caledonia
CAL
KU685761
–
–
–
Laccaria sp.
A4366
New Caledonia
CAL
KU685762
–
–
–
Coprinus comatus
AFTOL626
AY854066
AY635772
AY780934
AY881026
Cortinarius iodes
AFTOL285
AF389133
AY702013
AY536285
AY881027
Inocybe cookei
AFTOL520
DQ404391
AY702014
DQ385884
DQ435790
Lycoperdon pyriforme
AFTOL480
AY854075
AF287873
AY218495
AY883426
Mythicomyces corneipes
AFTOL972
DQ404393
AY745707
AFTOL972
DQ029197
Table S2b Agaricomycetideae dataset.
Genus species
Sample ID
Taxonomic group
18S
28S
RPB1
RPB2
EF1a
Coniophora arida
AFTOL 698
Boletales
AY293123
-
GU187492
DQ366282
DQ408146
Suillus pictus
AFTOL 717
Boletales
AY662659
AY684154
AY858965
AY786066
AY883429
Gomphidius roseus
AFTOL 1780
Boletales
DQ534682
DQ534669
GU187459
GU187818
GU187702
Mycena amabilissima
AFTOL 1686
Agaricomycotina
DQ457647
DQ457691
DQ447926
DQ474121
GU187727
Marasmius rotula
AFTOL 1505
Agaricomycotina
DQ113912
DQ457686
DQ447922
DQ474118
GU187723
Fibularhizoctonia sp
AFTOL 576
Athelioid (root)
AY654887
AY635779
AY857985
AY885161
AY879115
Inocybe cookei
AFTOL 520
Agaricomycotina
AY752967
AY702014
DQ447915
DQ385884
DQ435790
Inocybe dulcamara
AFTOL 482
Agaricomycotina
AY657016
AY700196
DQ447916
AY803751
DQ435791
Cortinarius iodes
AFTOL 285
Cortinariaceae
AY771605
AY702013
AY857984
AY536285
AY881027
Cortinarius aurilicis
AFTOL 812
Cortinariaceae
AY705957
AY684152
DQ083826
DQ083880
DQ061278
Galerina semilanceata
PBM 1398
Hymenogastraceae
DQ440639
AY038309
AF389531
AY337357
-
Hebeloma velutipes
AFTOL 980
Hymenogastraceae
AY752972
AY745703
DQ447904
DQ472718
-
Hebeloma olympianum
BK21_Nov_8_20/UTC/
Hymenogastraceae
-
AY038310
AF389532
AY337359
-
Agrocybe praecox
AFTOL 728
Strophariaceae
AY705956
AY646101
DQ516069
DQ385876
DQ061276
Hyphaloma fasciculare
PBM 1844
Strophariaceae
-
AY380409
AY351829
AY337413
-
Tubaria confragosa
AFTOL 498
Tubareae
AY665776
AY700190
DQ447944
DQ408113
-
Bolbitius vitellinus
AFTOL 730
Bolbitiaceae
AY705955
AY691807
DQ435802
DQ385878
DQ408148
Lycoperdon pyriforme
AFTOL 480
Agaricaceae
AF026619
AF287873
AY860524
AY218495
AY883426
Coprinus comatus
AFTOL 626
Agaricaceae
AY665772
AY635772
AY857983
AY780934
AY881026
Mythicomyces corneipes
AFTOL 972
Psathrellaceae
DQ092917
AY745707
DQ447929
Unpublished
DQ029197
Coprinopsis cinerea
MIX
Psathrellaceae
GenomeM92991
AF041494
Genome
Genome
-
Lacrymaria velutina
AFTOL 478
Psathrellaceae
AY654885
AY700198
-
DQ472733
-
Laccaria amethystina
LaAM-08-1
Laccaria (ingroup)
JGI
-
JGI
JGI
-
Laccaria galerinoides
F1080983
Laccaria (ingroup)
-
KU685776
-
KU685927
KU686077
Laccaria bicolor
S238
Laccaria (ingroup)
JGI
JGI
JGI
JGI
-
Laccaria ochropurpurea
AFTOL 477
Laccaria (ingroup)
AY654886
AY700200
-
DQ472731
-
Laccaria ambigua
PDD 89696
Laccaria (ingroup)
-
KU685876
-
KU686018
KU686132
Laccaria carneum
GMM7272
Laccaria (ingroup)
-
KU685820
-
KU685961
KU686094
Laccaria acanthospora
AWW485
Laccaria (ingroup)
-
JX504186
-
KU685916
KU686073

Notes S2 – Laccaria specimen and dataset information Wilson et al. Laccaria evolution
S2 page 9
Laccaria Thailand
DED7426
Laccaria (ingroup)
-
KU685771
-
KU685926
KU686076
Laccaria alba
AWW438
Laccaria (ingroup)
-
JX504178
-
KU685912
KU686072
Laccaria canaliculata
GMM7205
Laccaria (ingroup)
-
KU685800
-
KU685942
KU686085
Laccaria masonii
GMM7200
Laccaria (ingroup)
-
KU685799
-
KU685941
KU686084
Laccaria lilacina
GMM7531
Laccaria (ingroup)
-
KU685850
-
KU685992
KU686118
Laccaria spA
GMM7402
Laccaria (ingroup)
-
KU685832
-
KU685974
KU686106
Table S2c Dataset length statistics.
Dataset
Total Taxa
Total Char.
18S
ITS
28S
RPB2
RPB1
EF1a
Laccaria Systematics
237
3755
NA
780
768
1091
NA
1116
Global Laccaria*
116
3243
NA
676
732
1010
NA
825
Agaricomycetideae*
35
6626
1685
NA
1351
2075
573
942
*=introns removed from protein sequences
NA = region not used for dataset

Notes S3 – tMRCA priors Wilson et al. Laccaria evolution
Agaricomycetideae Dataset (Notes S3 Figure 1)
tMRCA
Node
Distribution
Initial
Value
Calibration
Mean*
Prior
Mean**
Log
(Stdev)
Offset
Mean in
Real Space
Reference
marasmioid
5
Lognormal
92
10
1
90
yes
Hibbett et al. 1997
Suillineae
3
Lognormal
55
10
1
50
yes
LePage et al. 1997
Global Laccaria Dataset (Fig. 1)
tMRCA
Node
Distribution
Initial
Value
Calibration
Mean*
Prior
Mean**
Log
(Stdev)
Offset
Mean in
Real Space
Reference
root
A
Lognormal
87
86.94
26
0.5
63
yes
Floudas et al. 2012
(node 15)
Hydnangiaceae
B
Lognormal
58
50.19
23
0.5
37
yes
Agaricomycetideae
Dataset
* This is the mean age, based on literature/analysis, used to calibrate the dataset
** This is the mean prior supplied to BEAUTi to approximate the Calibration Mean

Notes S4 – Results of tMRCA analyses Wilson et al. Laccaria evolution
1
Table S4a Dating results and age comparisons for Step 1 – Agaricomycetideae Dataset. (Notes S3)
Total Trees = 18002
likelihood = -44883.682 (ESS 1620)
Comparison to Floudas et al.
2012
Node
tMRCA
Mean
Median
95% CI
ESS
Node
AGA
BOL
ASC
95% CI
AGA
BOL
95% CI
AGA
ASC
95% CI
BOL
ASC
95% CI
1
Root -
Agaricomycetideae
145.8
143.59
117.7-
179.23
1285
13
148.94
117.17-
187.56
139.6
109.89-
176.71
152.95
117.84-
194.63
140.42
102.64-
185.11
2
Boletales
101.72
98.86
71.62-
136.72
479
16
104.23
73.93-
138.7
97.62
70.03-
129.44
110.18
78.12-
147.24
100.35
67.64-
136.98
3*
Suillineae
54.57
53.52
50.16-
61.90
4112
4
Agaricales
115.06
111.68
93.52-
144.35
739
14
122.74
97.46-
154.27
115.48
94.6-
144.78
125.12
98.12-
158.97
114.1
80.37-
154.32
5*
Marasmiaceae
101.83
98.03
90.19-
125.22
1141
6
Agariceae
64.47
63.70
36.10-
93.59
391
7
Cortinariaceae
31.75
30.36
15.53-
49.57
413
8
Inocybaceae +
Tubariaceae
60.09
58.97
42.32-
80.91
606
9
Inocybaceae
39.02
38.22
24.56-
56.01
299
10
Strophariaceae
77.06
75.53
56.05-
100.21
659
11
Psathyrellaceae
67.84
66.56
47.08-
90.98
708
A**
Laccaria+
Psathyrellaceae
86.26
84.39
65.16-
112.51
731
15
86.94
62.65-
115.18
82.39
59.38-
108.4
86.89
61.6-
117.1
81.09
51.78-
114.07
B**
Laccaria
(=Hydnangiaceae)
58.05
56.90
39.14-
79.82
786
C
Laccaria s.s.
minus Laccaria
ambigua
27.26
F
NH Laccaria
11.48
11.20
7.34-
16.17
242
* = Node used in calibration of the dataset.
** = tMRCA results used to calibrate step 2 analysis.
ESS = effective sample size. A value >200 is the
target for effective statistical sampling.

Notes S4 – Results of tMRCA analyses Wilson et al. Laccaria evolution
2
Table S4b Dating results for Step 2 - Global Laccaria Dataset. (Figure 1)
Total Trees = 49756
likelihood = -28609.301 (ESS 2851)
Node
tMRCA
Mean
Median
95% CI
ESS
A*
Root = Laccaria+
Mythicomyces
80.89
79.53
68.32-96.14
13367
B*
Laccaria
64.78
64.20
47.7-82.62
1722
C
Laccaria s.s. minus
Laccaria ambigua
43.14
42.49
29.42-58.46
669
D
36.62
36.03
24.75-49.79
634
E
31.69
31.17
21.02-43.05
581
F
NH Laccaria
24.17
23.77
15.81-32.94
587
G
NH Laccaria w/o SE
Asia
21.87
21.51
14.56-29.99
586
H
18.87
18.56
12.42-26.07
642
I
17.40
17.09
11.41-23.97
625
J
SE Asia Laccaria +
China
19.94
19.60
13.14-27.51
573
K
22.39
21.97
13.79-31.31
556
L
16.27
15.68
8.38-25.09
866
M
24.49
24.05
15.41-34.25
604
N
29.16
28.63
18.80-40.63
611
O
New Zealand Clade
18.31
17.87
9.28-28.19
615
P
27.36
26.70
17.00-39.79
406
* = Node used in calibration of the dataset.
ESS = effective sample size. A value >200 is the
target for effective statistical sampling.

Notes S5 – BAMM Statistics Wilson et al. Laccaria evolution
Notes S5b – 95% credible set of macroevolutionary rate configurations
(CSS) in Laccaria based on Bayes factors (sum f > 0.95). Letters on
phylogenies identify nodes associated with rate shifts.
Notes S5a – Most significant marginal
shift probabilities in Laccaria. Branch
lengths in phylogeny are proportional to
the probability of a rate shift along that
branch. Letters on phylogeny identify
nodes associated to branches with the
rate shift.

Notes S6 – BiSSE Results Wilson et al. Laccaria evolution
Notes S6 – a) Results of BiSSE analysis and contrasting diversification rates between
northern (red) and southern (blue) hemisphere Laccaria. a) Northern hemisphere
Laccaria speciation (a1) and diversification (a2) rates are elevated relative to southern
hemisphere Laccaria. b) Likelihood ratio test using 1000 posterior sampled trees from
BEAST analysis. Unconstrained speciation rates show a difference (mean P = 0.075,
green vertical line) from the constrained (null) model with equal speciation rates (b1)
using a P < 0.1. In contrast the model comparing diversification rates (b2) show a
greater difference (mean P < 0.05) between the unconstrained versus the constrained
models (mean P = 0.014).
Diversification rates (r) are measured as the difference between speciation (or lambda =
λ), and extinction (or mu = µ) rates (r = λ − µ). These rates are measured independently
for two different character states representing Southern Hemisphere (state 0) and
Northern Hemisphere (state 1) Laccaria. For example, lambda0 represents the Southern
Hemisphere speciation rate, while lambda1 represent the Northern Hemisphere
speciation rate. The value Rrel (rrel) is the relative diversification rate between the two
states (Rrel = Southern Hemisphere diversification rate / Northern Hemisphere
diversification rate = r0 / r1). An Rrel >1 signifies a faster Southern Hemisphere
diversification rate while <1 signifies a faster Northern Hemisphere diversification rate.

Notes S7 – Isotope data Wilson et al. Laccaria evolution
Table S7 – Specimen Isotope date generated for this study
Specimen ID
Taxa
Number
in figure
Site
Host
Ecology
δ13C
δ15N
PDD 89696
L. ambigua
1
Kauri Grove, Coromandel Peninsula
Leptospermum
unk
-21.96
11.56
F1080983
L. galerinoidies
2
Argentina
Nothofagus
L
-26.79
-0.51
F1081213
L. galerinoidies
3
Chile
Nothofagus
L
-26.75
4.36
PDD 89737
(KH_NZ06_119)
Laccaria NZ8
4
NZ, Mt Karioi, Waikato
Kunzea
L
-26.14
6.13
PDD 89685
(GMM7551 in
tree)
Laccaria NZ4
5
NZ, Chiltern Scenic Reserve, Coromandel
Peninsula
Kunzea,
Leptospermum
L
-25.26
5.39
GMM7400
L Q2
6
Aus: Queensland
Allocasuarina,
Eucalyptus
L
-26.43
1.11
GMM7411
L Q2
7
Aus: Queensland
Allocasuarina
L
-26.47
1.05
PDD 89677
(KH_NZ06_064)
Laccaria SpC2
8
NZ, Motutere, Coromandel Peninsula
Eucalyptus,
Leptospermum
L
-25.59
5.61
GMM7316
Laccaria PNG4
9
Paupua New Guinea
L
-25.10
1.48
PDD 89812
(KH_NZ06_197)
Laccaria NZ2
10
NZ, Waitakere Regional Park, Aukland
Leptospermum,
Agathis
L
-26.76
3.03
PDD 89739
(KH_NZ06_122)
Laccaria NZ3
11
NZ, Mt Karioi, Waikato
Kunzea
L
-25.30
6.01
GMM7301
L. PNG 3
12
PNG
Castanopsis,
Lithocarpus
L
-26.20
3.10
GMM7302
L. PNG 3
13
PNG
Castanopsis,
Lithocarpus
L
-26.10
4.24
GMM6776
Laccaria
14
China
L
-26.37
2.19
GMM7020
L. laccata s.s.
15
Russia
L
-23.33
2.16
AWW555
(SB2135 in tree)
Laccaria proxima
2
16
Magnolia Way, Nevada City, Nevada Co. CA
L
-24.11
5.43
AWW556
Laccaria
17
San Juan Ridge, Nevada City, Nevada Co.
CA
L
-24.18
5.69
AWW596
(HKAS44061 in
tree)
L. bicolor
18
Alaska, Highway 3, Mile 224, Carlo Creek
Lodge
Picea
L
-25.96
2.02

Notes S7 – Isotope data Wilson et al. Laccaria evolution
AWW572
L. JP4
19
USA: Michigan, UP
L
-25.79
1.24
GMM7028
L. macrocystidia
20
Russia
L
-25.72
0.81
AWW628
Armillaria
13894 Magnolia Way, Nevada City, Nevada
Co., CA., USA
Under Oak, Fir
nearby
s
-20.85
5.96
AWW626
Boletus
13894 Magnolia Way, Nevada City, Nevada
Co., CA., USA
Under Oak, Fir
nearby
m
-21.51
10.11
AWW659
Boletus
m
-23.52
9.74
PDD 89655
Hydnum
Thames, Coromandel Peninsula
m
-25.90
9.47
PDD 89697
Hygrocybe
Kauri Grove, Coromandel Peninsula
unk
-25.29
6.78
PDD 89743
Hymenogaster
Mt Karioi, Waikato
m
-25.12
7.59
PDD 89672
Hyphaloma
Coromandel, Coromandel Peninsula
s
-24.69
4.28
PDD 89798
Hysterangium
Waharau Regional Park
m
-26.73
9.54
GMM6788
Leucoagaricus
China
s
-22.06
5.28
AWW647
Mycetinus
Camp Augusta, Lawn area near climbing
wall, Nevada City, Nevada Co., CA
On lawn and under
pine.
s
-22.86
4.89
PDD 89704
Pluteus
Kauri Grove, Coromandel Peninsula
s
-25.56
2.49
AWW656
Russula
Camp Augusta, near corrals, Nevada City,
Nevada Co., CA
Under pine and
cedar
m
-25.18
5.75
GMM6781
Russula
China
m
-23.63
8.51
PDD 89621
Scleroderma
Waiomu Valley, Coromandel Peninsula
m
-26.53
6.58
PDD 89703
Stephanospora
Kauri Grove, Coromandel Peninsula
unk
-22.89
9.29
PDD 89810
Tympanella
Waitakere Regional Park, Aukland
s
-22.18
1.73
PDD 89639
Weraroa
Thames, Coromandel Peninsula
s
-25.53
1.13
Abbreviations under the “Ecology” column refer to: L = Laccaria ECM, m = mycorrhizal, s = saprotrophic, unk = unknown.
Stable isotope abundances are reported as: δ15N or δ13C (‰) = (Rsample/Rstandard -1) × 1000, where R is the ratio 13C/12C or
15N/14N. All δ13C and δ15N values were normalized on VPDB (δ13C) and AIR (δ15N) reference scales with laboratory
working standards of NIST 1515 (apple leaves), and tuna muscle, as well as an internal Boletus standard.

References
Floudas D, Binder M, Riley R, Barry K, Blanchette RA, Henrissat B, Martínez AT, Otillar
R, Spatafora JW, Yadav JS, et al. 2012. The Paleozoic origin of enzymatic lignin
decomposition reconstructed from 31 fungal genomes. Science 336(6089): 1715-1719.
Hibbett DS, Grimaldi D, Donoghue MJ. 1997. Fossil mushrooms from Cretaceous and
Miocene ambers and the evolution of homobasidiomycetes. American Journal of Botany
84: 981-991.
LePage BA, Currah RS, Stockey RA, Rothwell GW. 1997. Fossil ectomycorrhizae from the
Middle Eocene. American Journal of Botany 84: 410-412.