Practical aspects of estimation of optimal time for vaccination of chicken against IBD with use of "Deventer formula"

Abstract

One of the most commonly applied vaccination strategies against chicken infectious bursal disease (IBD) is the use of live vaccines at the optimal time estimated with the use of Deventer formula. The present study investigated the impact of different factors on maternally derived antibodies decline and therefore on the vaccination schedule against IBD. Our results suggest that blood collection from birds older than 3 days is more reliable in order to estimate the optimal date for IBD vaccination, due to disturbances in yolk sac resorption early after hatch.

Full text

DOI 10.1515/pjvs-2016-0053
Short communication
Practical aspects of estimation of optimal time
for vaccination of chicken against IBD with use
of „Deventer formula”
M. Śmiałek
1
, A. Śmiałek
2
, A. Koncicki
1
1
Department of Poultry Diseases, Faculty of Veterinary Medicine,
University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland
2
Private Veterinary Practice „Punkt Weterynaryjny”, Leszczynek 11A, 99-300 Kutno, Poland
Abstract
One of the most commonly applied vaccination strategies against chicken infectious bursal dis-
ease (IBD) is the use of live vaccines at the optimal time estimated with the use of Deventer formula.
The present study investigated the impact of different factors on maternally derived antibodies de-
cline and therefore on the vaccination schedule against IBD. Our results suggest that blood collection
from birds older than 3 days is more reliable in order to estimate the optimal date for IBD vaccina-
tion, due to disturbances in yolk sac resorption early after hatch.
Key words:chickens, Gumboro disease, vaccination, Deventer formula
Introduction
Infectious bursal disease (IBD), also known as
Gumboro disease, is a highly infectious, viral (IBDV
belongs to the Birnaviridae family) disease of
chickens. The greatest susceptibility to the clinical
course of IBD is observed in birds infected between
3 and 6 weeks of age (Mu¨ller et al. 2012) which results
from the maturation of B lymphocytes (target cells for
IBDV) in the bursa of Fabricius. While in older birds
the damage caused by IBD is less prominent, IBDV
infection of 3-6 week old and younger poults is re-
sponsible for immunosupression resulting from B cell
depletion (Mu¨ller et al. 2003, Withers et al. 2005).
Considering the above and IBDV worldwide distribu-
tion as well as their great resistance to environmental
Correspondence to: M. Śmiałek, e-mail: marcin.smialek@uwm.edu.pl
and chemical agents, IBD is one of the most economi-
cally important diseases of chickens.
A number of IBD vaccination strategies have been
applied in the field and new generation IBD vaccines
(vector and complex vaccines) are available on the
market (Mu¨ller et al. 2012). So far, one of the most
commonly applied vaccination strategies of broiler
chickens is the application of live conventional vac-
cines, characterized by different maternally derived
antibodies (MDA) titer break – through (most com-
monly 250 or 500). The basic assumption of „Deven-
ter formula” is that serological examination of chicken
poults enables determination of the optimal day for
vaccination of chickens against IBD with the use of
known MDA half-life (de Wit 2001). Recent studies
demonstrated the significance of Deventer formula
Polish Journal of Veterinary Sciences Vol. 19, No. 2 (2016), 425–427
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Table 1. Summary of origin of broiler chickens examined within the study.
Province Number of cases
Mazowieckie 76
Wielkopolskie 18
Warmińsko-Mazurskie 16
Świętokrzyskie 13
Łódzkie 11
Opolskie 2
Total 136
use in vaccination strategy against IBD (Block et al.
2007).
Materials and Methods
The study was carried out with broiler chicken
(from 136 broiler houses) blood samples (20-23 blood
samples from each broiler house were examined), col-
lected between September 2014 and July 2015. Each
broiler house originated from a different Polish breeder
flock situated in 6 different provinces (Table 1). Broilers
were hatched at 8 different commercial hatcheries.
Serum samples were stored at -20
o
C until analysis.
Serological examination of serum samples was
performed with a commercial ELISA kit specific for
detection of chicken anti-IBD antibodies (IDEXX,
USA) according to the manufacturer’s instructions.
ELISA was carried out using an Eppendorf epMotion
5075 LH automated pipetting station (Eppendorf,
Germany), BioTek ELx405 automatic plate washer
and BioTek ELx800 plate reader (BioTek, USA). Es-
timation of the optimal time for IBD vaccination with
the use of intermediate plus vaccine („500” – MDA
break-through titer = 500) was established according
to the basic assumptions of Deventer formula (all re-
sults had CV% <40) (de Wit 2001). The results were
expressed as percentages of cases of vaccination on
different days of broiler life. Results were also ana-
lyzed based on the birds age at blood sampling (0-3 or
4-7 days of life), the hatchery from which the birds
originated and the distance between the hatchery and
the broiler house (different ranges were considered:
0-60; 60-120; 120-180; 180-240; 240 km and more).
Results and Discussion
The general layout of the optimal day for vaccina-
tion of broiler chickens against IBD with the use of
intermediate plus vaccine is summarized in table 2.
Block et al. (2007) demonstrated that vaccination
against IBD 24 hours before or 3 days after the es-
timated optimal time for vaccination resulted in a sat-
isfactory serological response (14 days post vaccina-
tion) in evaluated chicken flocks. In the field „500”
vaccines are most often given to birds on the 16
th
day
of life, as a routine, in cases where Deventer formula
is not implemented. In the light of the above these
routine vaccinations would result in satisfactory vac-
cine-induced immunity in 66.39% of the analyzed
cases where birds were still protected by the MDA (in
regard to vaccinal MDA break-through; 16-17
th
day of
life) and in 97.79% of the cases in general (13-17
th
day
of life). On the other hand in 30.89% of the analyzed
cases, vaccination on the 16
th
day of life is later than
the estimated date of vaccination (10-15
th
day of life)
which generates a threat of production loss due to
early infection with field IBDV.
Since reports concerning the application of De-
venter formula in the field are scare our goal was to
investigate the possible impact of different factors
(hatchery practices, transport and accommodation
period) on MDA decline, and therefore the optimal
day for vaccination of broilers against IBD. The layout
of estimated optimal days for vaccination of broiler
chickens against IBD in regard to the sampling age is
summarized in table 2. In the light of the results of
Block et al. (2007), our findings indicate that the 16
th
day vaccination routine would result in satisfactory
vaccine-induced immunity in 79.01 and 54.71% of the
analyzed cases where birds were still protected by the
MDA (in regard to vaccinal MDA break-through;
16-17
th
day of life) and in 98.76 and 100% of the cases
in general (13-17
th
day of life) if blood samples were
collected 0-3 or 4-7 days after hatch respectively. The
above data indicate that the accommodation period
and other factors interfering with yolk sac resorption
influence the outcome of chicken anti – IBD serum
MDA level, which may affect vaccination schedule
and efficiency.
No differences have been observed in the distribu-
tion of optimal days for vaccination of broilers against
IBD in regard to the hatcheries and the distance be-
tween the hatchery and the broiler house (data not
shown) after implementation of sampling age-related
differences.
426 M. Śmiałek et al.
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Table 2. Layout of estimated days of vaccination of broiler chickens against IBD (based on „Deventer formula” protocol) within
analyzed cases.
Bird age at estimated time of vaccination Number of cases Percentage of cases
10 2 1.47
11 0 0
12 0 0
13 2 1.47
14 11 8.10
15 27 19.84
16 54 39.71
17 39 28.67
18 1 0.74
Total 136
Table 3. Layout of estimated days of vaccination of broiler chickens against IBD within analyzed cases, in regard to bird age at
time of blood sampling.
Percentage (number) of cases of vaccination against IBD at different age of birds – days of life
a
13 14 15 16 17 18
Birds age at blood
sampling
(number of cases)
0-3 (81) 0 1.23
(1) 18.52
(15) 44.44
(36) 34.57
(28) 1.23
(1)
4-7 (53) 3.77
(2) 18.87
(10) 22.64
(12) 33.96
(18) 20.75
(11) 0
a
Two cases in which vaccination was estimated on the 10
th
day of life were not included (blood samples in those cases were
collected on the 4
th
and 7
th
day of bird life).
In conclusion, Deventer formula is a powerful tool
which enables precise estimation of the day of vacci-
nation of chicken flocks against IBD. Despite the fact
that this formula enables blood sampling between
1-10 days after hatch, our results suggest that collec-
tion of blood samples from birds older than 3 days is
more accurate and reliable for estimating the optimal
date of IBD vaccination.
References
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