The diatoms of Kobylanka stream, a small watercourse near Kraków (Wyżyna Krakowsko- Częstochowska upland, S Poland), are described, illustrated with more than 1300 SEM and LM micrographs, and briefly discussed. Information on their ecology and their distribution generally, in Poland and on the Wyżyna Krakowsko-Częstochowska is provided for every taxon from Kobylanka stream. The 307 diatom taxa identified in the stream include 289 species of 64 genera, 16 varieties and two forms; 26 diatoms are identified only to genus level. The first Polish records of 27 species are reported: Achnanthidium jackii Rabhenhorst, Chamaepinnularia submuscicola (Krasske) Lange-Bertalot, Cymbella affiniformis Krammer, C. lange- bertalotii Krammer, Cymbopleura hercynica (A. Schmidt) Krammer, C. inaequaliformis Krammer, Diatoma moniliformis Kützing, Diploneis fontanella Lange-Bertalot, Encyonema lange-bertalotii Krammer, Gomphonema parvulum (Kützing) Rabenhorst var. parvulius Lange-Bertalot & Reichardt, G. parvulum var. parvulum f. saprophilum Lange-Bertalot & Reichardt, Luticola paramutica (Bock) D.G. Mann var. binodis (Bock) Van de Vijver, L. ventriconfusa Lange-Bertalot, Navicula vilaplanii (Lange-Bertalot & Sabater) Lange- Bertalot & Sabater, Nitzschia frequens Hustedt, N. tubicola Grunow, Pinnularia frequentis Krammer, P. isselana Krammer, P. kuetzingii Krammer, P. subcomutata Krammer, Planothidium minutissimum (Krasske) Morales, Sellaphora nana (Hustedt) Lange-Bertalot, Simonsenia delognei (Grunow) Lange-Bertalot, Stauroneis leguminopsis Lange-Bertalot & Krammer, S. prominula (Grunow) Hustedt, S. separanda Lange- Bertalot & Werum, Surirella terricola Lange-Bertalot & Alles. 47 diatoms are new to the Wyżyna Krakowsko- Częstochowska upland: Achnanthidium eutrophilum (Lange-Bertalot) Lange-Bertalot, A. minutissimum (Kützing) Czarnecki var. inconspicua Østrup, Amphora inariensis Krammer, A. montana Krasske, A. veneta Kützing, Caloneis aerophila Bock, Chamaepinnularia krookiformis (Krammer) Lange-Bertalot & Krammer, Cocconeis pseudolineata (Geitler) Lange-Bertalot, Craticula accomoda (Hustedt) D.G. Mann, C. molestiformis (Hustedt) D.G. Mann, Cylindrotheca gracilis (Brébisson) Grunow, Cymbella proxima Reimer, Cymbopleura subaequalis (Grunow) Krammer, Encyonema caespitosum Kützing, Eolimna subminuscula (Manguin) Moser, Lange-Bertalot & Metzeltin, Eunotia incisa Gregory, Fragilaria austriaca (Grunow) Lange-Bertalot, F. bidens Heiberg, Gomphocymbellopsis ancyli (Cleve) Krammer, Gomphonema sarcophagus Gregory, Grunowia sinuata Thwaites, Luticola acidoclinata Lange-Bertalot, L. goeppertiana (Bleisch) D.G. Mann, Mayamaea