Two new species of Calyptostoma Cambridge, 1875 (Acari: Prostigmata: Calyptostomatidae) from Cambodia and Sulawesi, Indonesia

Abstract

Calyptostoma giuliae sp. nov. from Cambodia and C. marantica sp. nov. from Sulawesi, Indonesia are described and illustrated from larvae collected from herbaceous plants. This is the first report of the genus Calyptostoma from Cambodia and Sulawesi, Indonesia. Calyptostoma velutinum is new to the fauna of Sicily and new to the fauna of Serbia. A key to the larval species of Calyptostoma of the world is given.

Full text

Systematic & Applied Acarology 20(8): 919–926 (2015)
http://dx.doi.org/10.11158/saa.20.8.7
919
© Systematic & Applied Acarology Society
ISSN 1362-1971 (print)
ISSN 2056-6069 (online)
http://zoobank.org/urn:lsid:zoobank.org:pub:9DB8D02E-837E-4EC4-AA1A-7C6C9D97BEA7
Two new species of Calyptostoma Cambridge, 1875 (Acari: Prostig-
mata: Calyptostomatidae) from Cambodia and Sulawesi, Indonesia
RYSZARD HAITLINGER¹ & MILOJE ŠUNDI ²
¹ Institute of Biology, Department of Invertebrate Systematics and Ecology, Wrocław University of Environmental and Life
Sciences, Ko
uchowska 5b, 51-631 Wrocław, Poland. E-mail: ryszard.haitlinger@up.wroc.pl
² University of Montenegro, Faculty of Natural Sciences and Mathematics, 2000 Podgorica, Montenegro.
E-mail: miloje@t-com.me
Abstract
Calyptostoma giuliae sp. nov. from Cambodia and C. marantica sp. nov. from Sulawesi, Indonesia are
described and illustrated from larvae collected from herbaceous plants. This is the first report of the genus
Calyptostoma from Cambodia and Sulawesi, Indonesia. Calyptostoma velutinum is new to the fauna of Sicily
and new to the fauna of Serbia. A key to the larval species of Calyptostoma of the world is given.
Key words: Taxonomy, Trombidiformes, Parasitengona, key, larva
Introduction
The genus Calyptostoma Cambridge, 1875 includes 4 species (based on larvae or larvae and adults):
Calyptostoma velutinum (Müller, 1776) widespread in Europe, found in Albania, Austria, Belgium,
Bulgaria, Czech Republic, Denmark, France, Germany, Great Britain, Greece, Hungary, Ireland,
Italy, Norway, Poland, Portugal, Russia, Sweden and Switzerland; it is known also from Japan
(Beron 2008, M
kol & Wohltmann, 2012), C. latiseta Shiba, 1976 based on adults and larvae from
Malaysia, New Caledonia and Tasmania, C. somplexa Shiba, 1976 based on larva from Malaysia and
C. gorganica Saboori & Soukhstaraii, 2012 based on larvae from Iran (Müller 1776, Koch, 1867,
Berlese, 1905, Shiba 1976, Beron 2008, M
kol & Wohltmann, 2012, Saboori et al. 2012).
In this paper, two new species of Calyptostoma from Cambodia and Indonesia, based on larvae,
are described. All specimens in this study were obtained from herbaceous plants, but some other
Calyptostoma species were reported as ectoparasite of Tipulidae (Diptera) (Wohltmann et al. 2007,
Saboori et al. 2012).
Material and methods
Four larvae of Calyptostoma giuliae sp. nov. were collected in Bantosey 20 km north of Sem Reap,
Cambodia and two larvae of C. marantica sp. nov. were collected in Marante, Sulawesi, Indonesia.
Specimens were preserved in 70% ethyl alcohol and later mounted on microscopic slides using
Berlese’s fluid. Measurements (given in micrometers) were made using microscope NIKON Eclipse
80i. Figures were drawn using Carl Zeiss Axiscop A1 microscope. The terminology and
abbreviations follows Wohltmann et al. (2007). The holotypes and paratypes of new species are
deposited at the Museum of Natural History, Wrocław University, Poland (MNHWU). All
specimens were collected by R. Haitlinger.
Article

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SYSTEMATIC & APPLIED ACAROLOGY VOL. 20
Calyptostoma Cambridge, 1875
Calyptostoma giuliae sp. nov. (Figs. 1–12)
Diagnosis: Larvae with following features (n=4): fnSo l: 10-8-4 (8–10, 8, 3–4 paratypes) , II 8-5-5
(7–8, 5, 5), III 1-3-3, fn Tr 1-1-2, fnBf 8-8-8, fnTf 9-12-5, microsetae present on Ti I and GeI-II with
two tips, all claws without prongs, Ta I 102–107. Ta III 92–106.
Description (n=4): larva.
Dorsum covered with very numerous short and nude setae. Scutum reduced, bearing one pair of
nude sensilla (broken in holotype). Below scutum, on each side, one pair of eyes placed on ovoid
sclerite, each with two nude setae. Anterior eye 28, posterior one 23, sclerite 56 long, 33 wide (Fig.
1).
FIGURES 1–3. Calyptostoma giuliae sp. nov. (larva). 1, dorsal view of idiosoma; 2, ventral view of idiosoma;
3, gnathosoma (dorsal view left, ventral view right).
Idiosoma ventrally covered with numerous short and nude setae. Anal opening without sclerite.
Three pairs of coxae present, each with numerous setae; Claperede’s organ is located in lateral
position between coxae I and II (Fig. 2). Length of setae on coxae I 19–32. Coxae II 31–38 and coxae
III 23–31.

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2015 HAITLINGER & ŠUNDI : TWO NEW SPECIES OF CALYPTOSTOMA
Gnathosoma ventrally with a pair of nude tritosternal setae bs (34), a pair of nude setae as (16)
and very short adoral setae (cs). Supracoxal setae (elcp) absent. Palpfemur with one nude seta placed
ventrally (25), two setae on palpgenu, ventral seta 23 long and dorsal seta 24 long. Palptibia with
three nude setae and short odontus (OD). Palptarsus with 2
, 1 and 4 nude setae (Fig. 3).
Legs. Leg setal formula. Leg I: Ta (covered with numerous smooth setae or almost smooth), 10
(8–10 paratypes), 2
, numerous N, Ti 8 , 1 , 16–17 N, Ge 4 (3–4), 1 , 15N, Tf 9N, Bf 8N, Tr 1N,
Cx 26–28N (Fig. 4).
Leg II: Ta 8 (7–8), 2 numerous N, Ti 5 , 14–15 N, Ge 5 , 1 , 10N, Tf 12N, Bf 8N, Tr 1N,
Cx 23–25N (Fig. 5).
Leg III: Ta 1 , , numerous N, Ti 3 , 14N, Ge 3 , 12N, Tf 5N, Bf 8N, Tr 2N, Cx 18N (Fig. 6).
Leg lengths. Leg I 512 holotype, 470–492 paratypes; leg II 460, 422–434; leg III 509, 445–453. IP
= 1481 holotype, 1345–1401 paratypes.
Measurements are given in Table 1.
FIGURES 4–6. Calyptostoma giuliae sp. nov. (larva). 4, leg I; 5, leg II; 6, leg III.
Etymology: The species is named after Giulia Spera, the senior author’s granddaughter.
Type material: The holotype and three paratypes (all larvae) were collected by R. Haitlinger
from herbaceous plants, 4 February 2002, in Bantosey, 20 km north of Siem Reap, Cambodia. All
type specimens are deposited in MNHWU.

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TABLE 1. Metric data of Calyptostoma guiuliae sp. nov. (1), C. marantica sp. nov. (2) and C. velutinum
(Müller, 1776) (3) larva; H—holotype, P—paratypes
.
Remarks: Calyptostoma giuliae sp. nov., differs from C. velutinum in fnTr III (2 vs. 1), Ta III
(1
vs. without ), gnathosoma with 3 pairs of setae vs. gnathosoma with one pair of setae, shorter
Ta I (102–107 vs. 126–163), Ta II (88–99 vs. 115–146), Ta III (92–106 vs. 110–158), Ti I (67–72
vs. 84–112) and IP (1345–1481 vs. 1671–2065); from C. simplexa in longer and higher Ta I (102–
Character H 1 1 1 1 1 1 P H 2 P 2 3
IL 1118 958 1075 876 916 1059 696–1524
IW 902 918 907 785 863 898 560–1153
L 29 26 28 32 33 34 27–40
W 66 56 53 63 65 62 57–73
SB 40 42 33–43
DS 32–41 28–40 31–41 34–40 24–35 27–37 25–56
as 20 22 19 29
bs 28 18 15 39 29–39
PaFe (L) 18
PaFe (W) 26
OD 10 12 8 12 12 15 3–6
PsFv 25 23 21 30 34
S 109 94 92 110
Ta I (L) 107 104 103 102 115 122 125–163
Ta I (H) 49 43 45 44 33 40 45–63
Ti I 72 69 68 67 83 83 74–104
Ge I 54 52 51 46 57 62 60–104
Tf I 39 34 29 34 44 36 28–49
Bf I 83 89 77 76 91 94 72–117
Tr I 39 35 31 32 37 48 30–42
Cx I 118 102 111 115 118 122 122–165
Ta II (L) 99 97 96 88 105 106 111–146
Ta II (H) 49 49 47 45 42 40 45–61
Ti II 53 59 56 54 65 69 59–93
Ge II 52 47 45 49 54 59 58–74
Tf II 36 27 27 28 39 36 37–44
Bf II 79 83 70 73 92 90 79–116
Tr II 34 36 33 34 51 65 34–49
Cx II 106 105 95 105 114 113 109–137
Ta III (L) 106 100 104 92 109 107 110–158
Ta III (H) 43 47 44 38 33 39 39–54
Ti III 75 62 65 63 82 77 80–107
Ge III 57 48 51 51 66 60 66–87
Tf III 37 33 31 30 41 34 40–57
Bf III 85 73 69 76 100 92 85–134
Tr III 40 34 32 33 44 47 34–49
Cx III 109 99 101 100 103 109 111–145
Leg I 512 498 470 472 545 567 545–708
Leg II 460 454 422 431 520 538 479–650
Leg III 509 449 453 445 545 526 542–707
IP 1481 1401 1345 1348 1610 1631 1565–2065

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2015 HAITLINGER & ŠUNDI : TWO NEW SPECIES OF CALYPTOSTOMA
107 vs. 92 and 43–49 vs. 29), Ti I (67–72 vs. 52), Ta II(H) (45–59 vs. 33), leg I (excluding coxae)
(359–394 vs. 275), leg II (327–354 vs. 250), leg III (345–400 vs. 273), dorsal setae (28–41 vs. 15),
dorsal setae with basal up-shaped stalks vs. setae without cup-shaped stalks, cuticle not reticulated
vs. cuticle reticulated and ocular plate with two setae vs. ocular plate without seta; from C. latiseta
in normal dorsal and ventral setae vs. leaf-shaped setae, shorter leg I (excluding coxae) (359–394 vs.
428), and Ta I (102–107 vs. 125); from C. gorganica in longer seta bs (32 vs. 20–21), shorter Ta I
(102–107 vs. 129–144, Ti I), Ti I (67–72 vs. 87–99), Ta II (88–99 vs. 111–124), Ta III (92–106 vs.
124–144), IP (1345–1491 vs.1772–1930), fnBf (8-8-5 vs. 16-9-7), fnTf (9-12-5 vs. 11-11-10) and
idiosoma without striations vs. idiosoma with striations and from C. marantica in the shorter IP
(1345–1481 vs. 1610–1631), Ta I (102–107 vs. 115–122), Ti I (67–72 vs. 83), Ge I (46–54 vs. 57–
62), Ta II (88–99 vs. 105–107), fnBf III (5 vs. 7), fnTf (9-12-4 vs. 8-8-7) and fn Tr I–II (1,1 vs. 2,2).
Calyptostoma marantica sp. nov. (Figs. 7–12)
Diagnosis: fnSol: I (5-6-3), II (5-5-5), III (1-4-2), fn Tr 2-2-2, fnBf 8-8-7, fnTf 8-8-7, microsetae on
tibia I and genu II with two tips, all claws without prong, Ta I 115–122, Ta III 107–109.
FIGURES 7–9. Calyptostoma marantica sp. nov. (larva). 7, dorsal view of idiosoma; 8, ventral view of
idiosoma; 9, gnathosoma (dorsal view left, ventral view right).

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SYSTEMATIC & APPLIED ACAROLOGY VOL. 20
Description (n=2): larva.
Dorsum covered with numerous short and nude setae. Scutum reduced bearing one pair of nude
sensilla. Below scutum, on each side, one pair of eye placed on ovoid sclerites, each with two nude
setae. Sclerite 48 long, 32 wide, anterior eye 15, posterior eye 23 (Fig. 7).
Idiosoma ventrally covered with numerous short and nude setae. Anal opening without
surrounding sclerite. Three pairs of coxae, each with numerous smooth setae. Length of coxal setae
I 22–32, II 21–31, III 20–30, number of coxal setae I 16, II 18, III 20. Claperede’s organ placed in
lateral position at coxa I (Fig. 8).
Gnathosoma ventrally with a pair of nude tritosternal setae (bs), a pair of nude oral setae (as)
and very short adoral setae (cs). Supracoxal setae absent. Palpfemur with one nude seta placed
ventrally (34), palpgenu with three nude setae (dorsal setae 28, 30, ventral seta 31). Palptibia with
3nude setae and short odontus. Palptarsus with 2
, 1 and 5 nude setae (Fig. 9).
Leg setal formula. Leg I: Ta 5 , 2 , numerous N, Ti 6 , 1 , 21, Ge 1 , 3 , 18N (20), Tf 8N, Bf
8N, Tr 2 (1N, 1B), Cx 18N (Fig. 10).
Leg II: Ta 5 , 2 , numerous N, Ti 5 , 23N, Ge 1 , 5 , 15N, Tf 8N, Bf 8N, Tr 2N, Cx 18N (Fig.
11).
Leg III: Ta 1 , 1 , numerous N, Ti 4 , 31N, Ge 2 , 10N, Tf 7N, Bf 7N, Tr 2N, Cx 20N (Fig. 12).
Leg lengths. Leg I 545 holotype, 567 paratype, II 520, 538, III 545, 526.
FIGURES 10–12. Calyptostoma marantica sp. nov. (larva). 10, leg I; 11, leg II; 12, leg III.

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2015 HAITLINGER & ŠUNDI : TWO NEW SPECIES OF CALYPTOSTOMA
IP = 1610 holotype, 1631 paratype
Measurements are given in Table 1.
Etymology: The species was named after the place where the holotype was collected.
Type material: The holotype and paratype larvae were collected by R. Haitlinger from
herbaceous plants, 18 February 2009 in Marante, Sulawesi, Indonesia. All type specimens in
MNHWU.
Remarks: Calyptostoma maranica sp. nov. differs from C. velutinum in number of subcapitular
setae (3 vs. 1), longer OD (12–15 vs. 3–6), fnTr (2-2-2 vs. 1-1-1), from C. latiseta in absence of leaf-
shaped setae on idiosoma and legs vs. leaf-shaped setae present on idiosoma and legs, Ta I
(5 vs.
1); from C. simplexa in longer idiosomal setae (24–37 vs. 15), number of subcapitular setae (3 pairs
vs. 1 pair), longer Ta I (115–122 vs. 95), Ti I (83 vs. 52), Ta II (105–106 vs. 80), leg I (545–567 vs.
275), leg II (520–538 vs. 250) and leg III (526–545 vs. 273); fn Ta I
(5 vs. 1), fn Ta II (5 vs. 1),
from C. gorganica in fnTr (2-2-2 vs. 1-1-2), fnBf (8-8-7 vs. 16-9-7), fnTf (8-8-7 vs.11-10-10),
shorter palpfemoralae (33–34 vs. 47–50), Ta I (L) (115–122 vs. 129–144), Ta I (H) (33–40 vs. 47–
52), leg I (545–567 vs. 613–655), leg II (520–538 vs. 540–615), leg III (526–545 vs. 619–660) and
IP (1610–1631 vs. 1772–1930).
Calyptostoma velutinum (Müller, 1776)
Measurements were obtained from specimens collected in Austria (1), Czech Republic (1), Serbia
(1), Poland (6) and Sicily (1).
Specimen from Sicily was collected in Chiusa Sclafani, Palermo province, 30 May 2012; coll.
R. Haitlinger. First record from Sicily. Specimen from Serbia was collected in Zlatibor, 3 August
2012; coll. M. Šundi
. First record from Serbia.
Key to larval species of Calyptostoma of the world
1. Gnathosoma with one pair of tritosternal setae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
- Gnathosoma with three pairs of tritosternal setae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2. Idiosoma and legs with a lot leaf-shaped setae . . . . . . . . . . . . . . . . . . . . . . . . . .C. latiseta Shiba; Malaysia
- Idiosoma and legs without such setae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
3. Idiosomal setae short < 20 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C. simplexa Shiba; Malaysia
- Idiosomal setae longer >24 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C. velutinum Müller; Europe, Japan
4. fnTr 2-2-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C. marantica sp. nov., Sulawesi, Indonesia
- fnTr 1-1-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
5. fnBf 8-8-5, fnTf 9-12-5 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C. giuliae sp. nov.
- fnBf 16-9-7, fn Tf 11-10-10 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C. gorganica Saboori, Soukhrsaraii; Iran
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Submitted: 24 Feb. 2015; accepted by Qing-Hai Fan: 10 Jun. 2015; published: 30 Nov. 2015