This study evaluated some of the important properties of medium density fiberboard (MDF) panels made from bamboo (Dendrocalamus asper) and rice straw. A total of 20 experimental panels with an average target density of 0.70 g/cm 3 were made from 100 percent bamboo, 100 percent rice straw and the mixture of 80 to 20 percent and 50 to 50 percent ratios of bamboo and rice straw fiber. Modulus of elasticity (MOE), modulus of rupture (MOR), internal bond strength, thickness swelling, density profiles, and surface roughness of the specimens were determined. Samples with 0.75 g/cm 3 density had 34 percent and 27 percent higher MOE and MOR values than those of samples with target density of 0.65 g/cm 3, respectively. It appears that using 5 percent of rice straw in the panels did not significantly reduce both physical and mechanical properties of the samples. Measurement of surface characteristics of the samples resulted that such panels can be used as substrate for thin overlays without any significant problems. Initial findings of this study suggest that both nonwood underutilized species can be considered as raw material to manufacture value-added MDF panels.