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Comparative Study on Blood Profiles of Indigenous and Ross-308 Broiler Breeders

Authors:
Bahman Abdi-Hachesoo at Shiraz University
Bahman Abdi-Hachesoo
Alireza Talebi at University of Tehran
Alireza Talebi
Siamak Asri-Rezaei at Urmia University
Siamak Asri-Rezaei
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Abstract

Some haematological parameters (Differential WBC counts, total white blood cell count (WBC) and packed cell volume (PCV) percentages) and biochemical values (total protein, alkaline phosphatase, alkaline transferase, calcium, phosphorous, magnesium, glucose, triglyceride and cholesterol) of adult indigenous chickens compared with adult industrial broiler breeders (Ross 308). Differences among hematological parameter of Ross-308 and indigenous cocks and Ross-308 and indigenous hens were not significant except for PCV% of Ross-308 (32.60 ± 2.32) and indigenous hens (35.50 ± 2.22) (P<0.016). Comparison of biochemical parameters, showed significant differences only for cholesterol and glucose values between indigenous and Ross-308 hens. Glucose values in Ross-308 hens was higher than indigenous hens (274.50 ± 33.22 mg/dl and 245.60 ± 28.11 mg/dl, respectively) (P<0.026). In addition, cholesterol values in Ross-308 hens was higher than indigenous hens (181.50 ± 33.22 mg/dl and 152.60 ± 28.11 mg/dl, respectively) (P<0.026). No significant differences were observed between biochemical parameters values of Ross-308 and indigenous cocks.
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Global Veterinaria 7 (3): 238-241, 2011
ISSN 1992-6197
© IDOSI Publications, 2011
Corresponding Author: Bahman Abdi-Hachesoo, Department of Clinical Science, Poultry Diseases Division,
School of Vet. Med., Shiraz University. P.O. Box 1731, Postal Code 71345, Iran.
238
Comparative Study on Blood Profiles of
Indigenous and Ross-308 Broiler Breeders
Bahman Abdi-Hachesoo, Alireza Talebi and Siamak Asri-Rezaei
1 2 3
Department of Avian Medicine,
1
School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Department of Clinical Science, Poultry Diseases Division,
2
School of Vet. Medicine, Urmia University, Urmia, Iran
Department of Clinical Science, Clinical-Pathology Division,
3
School of Vet. Medicine, Urmia University, Urmia, Iran
Abstract: Some haematological parameters (Differential WBC counts, total white blood cell count (WBC) and
packed cell volume (PCV) percentages) and biochemical values (total protein, alkaline phosphatase, alkaline
transferase, calcium, phosphorous, magnesium, glucose, triglyceride and cholesterol) of adult indigenous
chickens compared with adult industrial broiler breeders (Ross 308). Differences among hematological parameter
of Ross-308 and indigenous cocks and Ross-308 and indigenous hens were not significant except for PCV%
of Ross-308 (32.60 ± 2.32) and indigenous hens (35.50 ± 2.22) (P<0.016). Comparison of biochemical parameters,
showed significant differences only for cholesterol and glucose values between indigenous and Ross-308 hens.
Glucose values in Ross-308 hens was higher than indigenous hens (274.50 ± 33.22 mg/dl and 245.60 ± 28.11
mg/dl, respectively) (P<0.026). In addition, cholesterol values in Ross-308 hens was higher than indigenous
hens (181.50 ± 33.22 mg/dl and 152.60 ± 28.11 mg/dl, respectively) (P<0.026). No significant differences were
observed between biochemical parameters values of Ross-308 and indigenous cocks.
Key words: Haematological Parameters % Leukogram % Biochemical Parameters % Indigenous Chicken % Ross
308 Broiler Breeders
INTRODUCTION MATERIALS AND METHODS
In many species of birds, normal values forChickens: Twenty indigenous chickens (10 hens and
hematological and biochemical factors were measured and 10 cocks, with the age of 18 weeks) were purchased from
a comprehensive data base was established as theirindigenous poultry research center of Urmia agricultural
blood-profiles. Comparative measurements for diverseoffice, west Azerbaijan, Iran. Also, twenty industrial
species of birds could be lead to the different statementschickens Ross-308 (10 hens and 10 cocks, with the age of
of immune system in these birds. In recent years, new18 weeks) were purchased from a breeder broiler farm in
researches focuses on village chickens has beenUrmia city. These chickens transmitted to the Faculty of
developed in many African and Asian countries [1-4]. InVeterinary Medicine, Urmia University and were housed
the other hand, many researchers have evaluated normalin four separate groups with same rations and
biochemical and hematological parameters of industrialenvironmental conditions for eight weeks and received
and commercial hybrid chickens [5,6].but there is a little of no vaccines in the experimental period. After eight
information about compression of blood profiles between weeks, blood samples including 2ml of blood for serum
indigenous and industrial chickens. Therefore, this studybiochemical tests and 1ml of blood in vials with 2mg
was carried out to compare hematological and serum'sethylene-diamine tetra-acetic acid (EDTA) for
biochemical values between industrial (Ross-308)haematology were collected from the brachial vein as
and Azerbaijan indigenous chickens (Gallus domesticus). described [7].
Global Veterinaria, 7 (3): 238-241, 2011
239
Haematology: Differential white blood cell count [ WBC] As shown in table 1, results of present study showed
counts were made on monolayer blood films, fixed andthat there are significant statistical differences between
stained with Giemsa-Wright’s stain. Total (WBC) countblood profiles of indigenous and Ross-308 adult chickens
was determined by a manual method usingas follow:
haemacytometer. Packed cell volume (PCV) was measured
by a standard manual technique using microhaematocritHaematological Parameters
capillary tubes and centrifuged at 2500 rpm for 5 min [8]. Cocks of Indigenous and Ross-308: No significant
Biochemical Parameters: Serum biochemical parameters parameters for cocks of indigenous and Ross-308
including total protein (TP), alkaline phosphatase (ALP),chickens.
alkaline transferase (ALT), calcium, phosphorous,
magnesium, glucose, triglyceride and cholesterol) wereHens of Indigenous and Ross-308: Comparison of
determined in a Technicon RA 1000 Autoanalyzerhaematological parameters of hens of indigenous and
(Technicon Instruments Corporation, Tarrytown, NewRoss-308, showed significant difference (P<0.016) only for
York, USA). PCV values but not showed for others parameters.
Statistical Analysis: All values of hematological andBiochemical Parameters:
serum's biochemical values were expressed as mean ±Cocks of Indigenous and Ross-308: No significant
standard deviation. Two-tailed student test from SPSSdifferences were observed among values of biochemical
were used for statistical analysis of the results. Theparameters for cocks of indigenous and Ross-308
level of significance was reported at less than P < 0.05 [9]. chickens.
RESULTS Hens of Indigenous and Ross-308: Comparison of values
The results of haematological and biochemical308, showed significant differences only for cholesterol
parameters were given in mean ± standard error means for and glucose values (P<0.026 and P<0.026, respectively)
each value in Table 1. but not showed for other parameters.
differences were observed among values haematological
biochemical parameters for hens of indigenous and Ross-
Table 1: Comparison of blood profiles for indigenous and Ross 308 adult-chickens
Indigenous Ross-308
----------------------------------------------------- --------------------------------------------------------------
Parameters Cock Hens Cock Hens
Haematocrit (%) 46.10 ± 2.85 35.50 ± 2.22 48.80 ± 4.31 32.60 ± 2.32
a b
WBC (×10³µl) 9920 ± 1560.66 8885 ± 1850.39 10220 ± 1648.43 8850 ± 2129.72
Heterophil (%) 28.30 ± 3.77 38.60 ± 2.95 30.20 ± 5.39 37.80 ± 4.82
Lymphocyte (%) 65.39 ± 4.50 51.90 ± 4.46 61.60 ± 6.60 53.00 ± 4.14
Monocyte (%) 3.40 ±1.07 3.90 ± 1.37 3.70 ±1.16 3.70 ± 1.34
Eosinophil (%) 2.60 ± 1.26 3.50 ± 1.43 3.40 ± 1.17 3.40 ± 1.50
Basophil (%) 0.40 ± 0.52 2.10 ± .99 1.1 ± 1.1 2.10 ± 0.99
Albumin (g/dl) 2.77± .70 4.12 ± 0.46 2.43± 0.42 3.86 ± 0.76
ALT (IU/L) 7.80 ± 1.62 7.20 ± 1.47 10.20 ± 2.25 7.90 ± 1.91
AST (IU/L) 191±.89 125.20 ± 11.76 198.40 ± 14.06 119.00 ± 13.32
Total Protein (g/dl) 3.87 ± .70 5.22 ± 0.46 3.53 ±0 .42 4.96 ± 0.76
Glucose (mg/dl) 260.60 ± 35.68 245.60 ± 28.11 260.50 ± 18.71 274.50 ± 33.22
a b
Triglyceride (mg/dl) 79.20 ± 14.57 64.80 ± 13.28 89.90 ± 13.08 71.10 ± 17.21
Cholesterol (mg/dl) 167.60 ± 35.68 152.60 ± 28.11 74.50 ± 18.71 181.50 ± 33.22
a b
Calcium (mg/dl) 11.32 ± 1.54 9.36 ± 0.58 12.19 ± 1.23 9.28 ± 0.91
Phosphorous (mg/dl) 5.07 ± 0.69 4.23 ± 0.26 5.57 ±0 .56 4.44 ± 0.43
Magnesium (mg/dl) 1.99 ± 0.27 1.65 ± 0.10 2.15 ± 0.2 1.64 ± 0.16
Different letters in row indicate significance at level P<0.05
Global Veterinaria, 7 (3): 238-241, 2011
240
DISCUSSION between indigenous and Ross 308 hens. Indigenous hens
Blood profiles to be a reliable health indicator. ItsRoss 308 hens. Other haematological and biochemical
variation should display a consistent pattern, with theparameters between indigenous and Ross308 broilers
inter-brood variance component [10]. Hematological andhadn’t significant differences.
biochemical parameters in indigenous chickens in various
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  • Feb 2004
  • Int J Poultry Sci
An experiment was conducted to study the hematological parameters in Fayoumi, Assil and Local Chickens of different ages reared in Sylhet region. 250 chickens of three breeds (100 Fayoumi, 50 Assil and 100 Local) were tested at different ages (1, 3, 6, 9 and 12 months) to observe the hematological parameters: i) Total erythrocyte Count (TEC) ii) Packed Cell Volume (PCV) iii) Determination of Hemoglobin (Hb) iv) Erythrocyte Sedimentation Rate (ESR) v) Differential Leukocyte Count (DLC) vi) Mean Corpuscular Volume (MCV) vii) Mean Corpuscular Hemoglobin (MCH) viii) Mean Corpuscular Hemoglobin Concentration (MCHC). Erythrocyte numbers, hemoglobin concentration and packed cell volume increased with the advancement of age in all three breeds. The TEC was higher in Fayoumi. The hemoglobin concentration was high in Assil. The PCV was slightly different or similar in all three breeds. ESR was inversely related to the age. Higher ESR in early age and lower in the advancement of age. The Fayoumi showed the higher ESR compared to other two breeds. The ESR of last two groups (9 and 12 months) of Assil and all groups of Local Chickens were negligible. Lymphocytes and heterophils were two principal leukocytes, which exert their dominance on other leukocytes. Among three breeds Local chickens possess the higher lymphocyte percentage. The heterophils were higher in Fayoumi breed. Monocyte was lower in Assil and Local chickens. Eosinophils were higher in number in local and Assil compared to Fayoumi. Higher MCV was recorded in Local chickens followed by Assil and Fayoumi chickens. MCH values were near about similar in Assil and local chicken but lower in Fayoumi breeds. The MCHC values of all three breeds were almost nearer to each other. The above study has highlighted some of the normal hematological parameters of chicken mostly reared in Sylhet region. However, more detailed study could be conducted in this regards.
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Performance and physiological responses of naked-neck chickens and their F 1 crosses with commercial layer breeds to long-term high ambient temperature
Article
Full-text available
  • Jan 2011
An experiment was conducted on forty-eight female chickens from each of the following genotypes: Naked-neck (Na, from Ethiopia), New Hampshire (NH), Lohmann White (LW) and F 1 crosses of NaxNH and NaxLW. Twenty four chickens from each genotype were randomly divided and assigned either to high (30-32 °C) or normal ambient temperature (18-20 °C). Body weights were measured during 20 and 68 weeks of age. Eggs were collected once daily while egg weight and feed intake were determined at 28-d intervals. Blood samples were taken from 12 randomly selected birds per genotype and ambient temperature at 38, 51 and 65 weeks age. Total protein levels were measured in plasma whereas differential leukocyte counts were determined from whole blood. Compared with controls, the egg production and feed consumption in commercial layers significantly reduced by 24.2 and 15%, respectively. Surprisingly, heat stress favourably increased body weight in NaxLW genotype by 1.7 and 2.8 % at 20 and 65 weeks, respectively and appeared to be the best F 1 cross combinations. 1 The effect of heat stress was significant for all differential leukocyte counts. Compared with controls, the respective increase in heterophil, basophil and monocyte counts was 19.2, 43.6 and 41% whereas the lymphocyte and eosinophil numbers reduced by 9 and 11.6%, respectively. The hetrophil:lymphocyte (H/L) ratio increased by 45, 18 and 19% at 38, 51 and 65 weeks age, respectively. At high temperature, the highest lymphocyte counts were noted in LW and the lowest in NaxNH whereas heterophil counts were highest in NH and NaxNH resulting in increased H/L ratio. The total plasma protein reduced in all heat stressed chickens although it was only significant at 38 weeks age. It was inferred that the total plasma protein mean values in heat stressed hens declined from 4.12 g/dl at 38 weeks to 2.22 g/dl at 65 weeks. In conclusion, the Na and its F crosses demonstrated better heat stress tolerant than commercial layer chickens as evidenced by measured 1 performance and physiological parameters. These results suggested that differential leukocyte counts, H/L ratio and total plasma protein could be used as a reliable indicator of long-term heat stress in layer chickens.
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Period of incubation and posthatching holding time influence on broiler performance
Article
Full-text available
  • Jul 2006
The present study had the objective of investigating the performance of broilers housed immediately after hatching or after a 12 or 24 hour of post-hatching holding time. One thousand and six hundred male Ross 308 broiler chicks with an initial body weight of 46 grams were used. These chicks were distributed in a completely randomized experimental design with 5 treatments and 8 replications of 35 birds in each treatment. The treatments in this study consisted of the removal of chicks from the hatchery in three different times: after 480, 492 and 504 hours of incubation. In each one of these times, 280 chicks were removed from the hatchery and immediately housed. Another group of an identical number of chicks of each time remained in the hatchery to be housed at 504 hours after hatching. The chick group corresponding to those hatched at 480 and 492 hours performed better until 7 days. However, no differences in body weight or body weight gain were observed at the end of the study. Feed efficiency, however was worse for the birds hatched and placed at 480 hours. There were no differences among treatments for mortality.
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Serum Lipid, Growth and Haematological Parameters of Broilers Fed Garlic, Ginger and Their Mixtures
Article
Full-text available
  • Jan 2009
The study determined the effects of garlic, ginger and their mixtures on the growth, serum lipids, haematological indices, carcass and organs of broilers. Garlic and ginger were each added to broiler diets at 1, 1.5 and 2%. Four mixtures of garlic and ginger were also formulated. Control diet did not contain any test ingredient. The results showed that garlic, ginger and mixtures contained 7.41, 5.85 and 6.62% crude protein respectively. Dietary treatment significantly influenced the final liveweights (FLW) of broiler chicks (P
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Haematological Studies in Adolescent Breeding Cocks
Article
Full-text available
  • Sep 2000
The objective of the study was to point out several significant changes in haematological values of male poultry occurring during sexual maturation and development of spermiogenesis using chickens (Gallus gallus domesticus L.) as a model species. The investigations were carried out in a set of 50 clinically normal breeding cockerels of the paternal line RIR-05 and covered the age span of 10 through 35 weeks. Blood samples collected at 5-week intervals from vena basilica were tested for total erythrocyte count (Er), packed cell volume (PCV), haemoglobin content (Hb), mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin content (MCH), mean erythrocytic volume (MCV), and total leukocyte (Le), basophil (Ba), eosinophil (Eo), neutrophil (Ne), lymphocyte (Ly), and monocyte (Mo) counts. Changes in the erythrocytic component observed during the investigation period included highly significant (P less than or equal to 0.01) increases in Er from 1.793 to 3,484 T (.) l(-1), PCV from 0.243 to 0.4351 (.) l(-1), and Hb from 69.94 to 136.57 g (.) l(-1) . No significant changes were demonstrated for MCHC, MCH, and MCV in which mean values ranged from 26.17 to 31.531 (.) l(-1), from 36.39 to 44.20 pg, and from 125.61 to 157.86 fl, respectively. A reverse tendency was observed in the leukocytic component. Le decreased highly significantly (P less than or equal to 0.01) from 20.270 to 12.788 G (.) l(-1). For this change, highly significant decreases in Pa from 0.138 to 0.065 G (.) l(-1), Eo from 0.0-104 to 0.063 G (.) l(-1), and mostly Ly from 11.502 to 3.784 g (.) l(-1) (P less than or equal to 0.01) and Mo from 0.104 to 0.063 G (.) l(-1) were responsible. Ne remained fairly constant varying within a narrow range of 7.742 to 8.626 G (.) l(-1). The paper provides new comprehensive information on haematological changes occurring during sexual maturation of male breeding chickens up to the onset of reproductive activity.
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Haematological and Serum Biochemical Indices of Naked Neck and Normally Feathered Nigerian Indigenous Chickens in a Sub Humid Tropical Environment
Article
Full-text available
  • Jan 2008
  • Int J Poultry Sci
Haematological and serum biochemical indices of Nigerian indigenous chickens of two genetic groups were evaluated. One hundred and twenty (120) cocks with average weight of 1.10kg, corresponding to sixty (60) naked neck (NaNa) and sixty (60) normally feathered (nana) were utilized in the study. On the basis of feather colour, the normal plumage cocks were subdivided into two equal phenotypic groups. Each genetic group was divided into four replicates of fifteen birds each in a completely randomized design. There were no significant (P>0.05) differences between the genotypes in the mean values of white blood cells(WBC), mean corpuscular volume(MCV), mean corpuscular haemoglobin concentration(MCHC). However, naked neck cocks were significantly superior in packed cell volume (PCV) haemoglobin (Hb) and red blood cells(RBC) compared with their normally feathered counterparts(41.00 vs 35.90%; 13.68 vs 11.60 g/dl; 4.84 vs 4.21x10<SUP>6</SUP>/ml; p>0.05). With the exception of PCV which was significantly (p>0.05) higher in brown cocks than white coloured cocks (38.30 vs 33.50%), plumage colour had no significant effect on the variables estimated. No significant differences were observed in total protein, albumin, urea, glucose, cholesterol, serum alanine amino transaminase (SALT) and serum aspartate amino transferase (SAST) of the genetic groups. The normally feathered cocks, however had significantly (P<0.05) higher globulin content (1.53 vs 1.15 g/dl) and lower creatinine value (0.88 vs 0.95 mg/dl) compared to the naked neck cocks. Plumage colour did not significantly (P>0.05) affect serum biochemical parameters suggesting that the two colour variants might not be true representation of genetically distinct subpopulations. The present result on blood parameters of both naked neck and normally feathered cocks could serve as a baseline data, which could be exploited in the improvement of local stock.
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Blood Cell Characteristics and Hematological Values of Free Ranging-Red Jungle Fowl ( Gallus gallus ) in Northeastern, Thailand
Article
  • Apr 2007
Blood cell characteristics and hematological values were studied in thirty-three healthy free ranging-red jungle fowl ( Gallus gallus ) (male, n = 17; female, n = 16), at the age of 2-3 years, which had been free ranged at Nakhonratchasima Zoo, Nakhon Ratchasima province northeastern, Thailand. The results revealed the following information: the morphologic features of blood cells from red jungle fowls were similar to those of other species of chicken. TRBC, Hb, PCV, MCV, MCH, MCHC, TWBC, lymphocyte, heterophil, monocyte, eosinophil, basophil, thrombocyte of Red Jungle Fowl were 4.43±0.67 (3.76-5.10) x10<sup>6</sup> cells μL•<sup>-1</sup>, 13.63±2.28 (11.35-15.91) g %, 41.34±6.71 (34.63-48.05) %, 93.57±7.97 (85.60-101.54) fl, 30.80±2.24 (28.56-33.04) g dL•<sup>-1</sup>, 30.41±1.60 (28.81-32.01) pg, 9,140±3,839.27 (5,300.73-12, 979.27) cells /μL, 74.6±20.27 (54.33-94.87) %, 21.83±19.18 (2.65-41.01) %, 2.00±1.47 (0.53-3.47) %, 3.50±1.34 (2.16-4.84) %, 1.67±2.79 (0-4.46) %, respectively. Width and length of red blood cell of red jungle fowls were 6.70±0.57 and 10.65±0.59 μm. Diameter of heterophil, lymphocyte, monocyte, eosiniphil, basophil and thrombocyte of red jungle fowls were 10.85±0.75, 5.60±0.48, 9.35±1.04, 8.25±0.91, 7.63±1.40 and 4.25±0.72 μm, respectively. Moreover, total red blood cell, hemoglobin concentration and packed cell volume of males red jungle fowl were higher than females (p<0.05). Finally, MCV, MCH and MCHC of the male and female red jungle fowl were not significantly different (p>0.05).
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Haematology, Serum Chemistry and Electrocardiographic Evaluation in Native Chicken of Kashmir
Article
  • Aug 2007
  • Int J Poultry Sci
Basic physiology, hematology and serum chemistry of indigenous chicken, reared in eco-friendly environment of backyard of small farmers in temperate regions of J and K State (altitude range 1500-2000 m amsl, average temperature 13°C), was studied in adult chicken from different pockets of Kashmir. The average values for haemoglobin (Hb), Packed Cell Volume (PCV), Total Leukocyte Count (TLC), Total Erythrocyte Count (TEC), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC) were 13.21±0.39g%, 40.70±0.51%, 16.80±0.47×10<SUP>3</SUP>/cumm, 3.20±0.07×10<SUP>6</SUP>/cumm, 127.18±2.68fl, 41.28±0.56pg, 32.45±0.57g% and 11.32±0.19g%, 35.21±0.61%, 22.29±0.37×10<SUP>3</SUP>/cumm, 2.98±0.08×10<SUP>6</SUP>/cumm, 118.15±1.76 fl, 37.98±0.56pg, 32.14±0.39g% in cock and hen respectively. Percentage presence of different leukocytes in blood was 32.55±0.61, 48.87±0.81, 10.90±0.35, 5.67±0.24, 2.05±0.18 and 26.32±0.46, 49.98±0.72, 10.85±0.36, 8.69±0.26, 3.19±0.23 for heterophils, lymphocytes, monocytes, eosinophils, basophils respectively in cock and hen. The cytometry of erythrocytes revealed 13.86±0.11 micrometre and 12.78±0.09 micrometre length, 8.37±0.09 micrometre and 7.86±0.07 micrometre breadth in cock and hen respectively. The values for Hb, PCV, TLC, TEC and RBC size differed significantly (p<0.01) among the cocks and hens. Serum was analysed for glucose, cholesterol, total protein, albumin, globulin, uric acid, creatinine, calcium, phosphorus and alkaline phosphatase, the average values recorded were 227.83±6.07 mg dL<SUP>-1</SUP>, 116.33±4.90 mg dL<SUP>-1</SUP>, 3.99±0.18 g dL<SUP>-1</SUP>, 1.78±0.06 g dL<SUP>-1</SUP>, 2.15±0.17 g dL<SUP>-1</SUP>, 6.31±0.33 mg dL<SUP>-1</SUP>, 1.54±0.17 mg dL<SUP>-1</SUP>, 10.79±0.50 mg dL<SUP>-1</SUP>, 4.19±0.20 mg dL<SUP>-1</SUP>, 9.84±0.67 K.A.U and 227.43±4.93 mg dL<SUP>-1</SUP>, 141.02±5.36 mg dL<SUP>-1</SUP>, 4.97±0.16 g dL<SUP>-1</SUP>, 2.20±0.07 g dL<SUP>-1</SUP>, 2.79±0.14 g dL<SUP>-1</SUP>, 6.21±0.40 mg dL<SUP>-1</SUP>, 1.62±0.08 mg dL<SUP>-1</SUP>, 12.01±0.47 mg dL<SUP>-1</SUP>, 4.74±0.30 mg dL<SUP>-1</SUP>, 11.11±0.84 K.A.U in cock and hen respectively. The values for cholesterol, total protein and albumin differed significantly (p<0.01) among the sexes where as no significant difference for glucose, uric acid, creatinine, calcium, phosphorus or alkaline phosphatase was observed. Besides haematology and serum chemistry, heart rate, respiration rate and rectal temperature were recorded and the values were 285.80±6.78 beats/min and 359.92±8.48 beats/min, 27.29±0.49/min and 31.10±0.32/min, 105.59±0.14<SUP>o</SUP>F and 106.31±0.09<SUP>o</SUP>F in cock and hen respectively that differed significantly (p<0.01) among sexes. Mean electrical axis of QRS was -81.4±1.97<SUP>o</SUP> and -78.38±1.76<SUP>o</SUP> in cock and hen respectively. The time intervals of different phases of cardiac cycle in cock and hen were 205 ms and 169 ms, 59.16 ms and 49.44 ms, 102.5 ms and 87.77 ms and 23.75 ms and 12.5 ms for R-R, P-R, R-T and T-P intervals respectively. The study revealed significant effect of sex on various physiological parameters in native chicken of Kashmir.
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Comparative Studies on Haematological Values of Broiler Strains (Ross, Cobb, Arbor-acres and Arian)
Article
  • Aug 2005
  • Int J Poultry Sci
Measurement of haematological parameters provides valuable information for evaluation of health status of man and animals, but due to lack of reference values for avian blood profiles, it is not widely used in poultry. This study has contributed to the current knowledge on avian blood profiles, or more specifically demonstrated leukograms and haemograms of four main broiler strains (Ross, Cobb, Arbor-Acres, and Arian) during 8 weeks husbandry period. Blood samples were collected from chickens at different age (1 to 56 days old) with 7-day sampling intervals. Total numbers of RBC and WBC, PCV, Hb, MCV, MCH, MCHC, ESR, together with absolute count of heterophils, lymphocytes, monocytes, eosinophils, and basophils as well as H / L ratio were determined. One-way ANOVA, Wilcoxon, Spearman correlation and Kruskal-Wallis H tests from SPSS11 were used for statistical analysis of the results. This study showed that age affects significantly (P< 0.05) the haematological profiles of the broiler strains. With increasing of age, the erythrocytic parameters (except MCV, MCH, and MCHC) and leukocytic parameters (except heterophils and H / L ratio) were significantly increased (P< 0.01), but MCV, MCH and absolute count of heterophils as well as H / L ratio were significantly decreased (P< 0.01).
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