This study explores little-recognized subject of microbiological dependencies occurring in the regions where groundwater drainage occurs. The main goal is to follow variability of prokaryotic organisms (bacteriocenosis) which inhabit the shallow littoral area of the Gulf of Puck and also to characterize the occurrence of underwater groundwater drainage in the area with the environmental conditions affecting it. There were no studies of this type in the coastal zone of the sandy beaches of the Baltic Sea so far.
On the basis of the literature data, the hydrogeological and physicochemical characteristics of the phenomenon were presented, as well as the research area, which was a shallow littoral and sandy beach zone under the influence of groundwater drainage and without this influence. The research was conducted in the vicinity of the city of Hel, located at the eastern end of the Hel Peninsula.
Using microscopic, cultivation and genetic methods, the number, size structure and biomass of prokaryotic cells were analyzed. Their metabolic activity and taxonomic differentiation were determined. Due to the recreational character of the area, special attention was given to the possible impact of groundwater drainage on sanitary quality of coastal waters. The results of the research confirm that in environmental studies auto- and allochthonous bacteriocenosis develops under the influence of numerous factors, most of which can be described as "environmental stress".
It was concluded that although the inflow of groundwater in the coastal area does not cause significant changes in the number, size and biomass of bacterial cells settling in coastal sea waters and sandbanks, it does affect their activity and diversity. The intensity of these changes is however dependent on the season (mainly temperature and oxygenation). The interdependence between the availability of oxygen the value of redox potential and biochemical processes catalyzed by microorganisms with specific life requirements has been observed. It has been demonstrated, among others, that selected groups of microbiological indicators, e.g. Archaea methanogenic, identified by genetic methods (NGS) may be a sufficiently sensitive bioindicator of the impact of groundwater drainage on the marine environment.
Recognition of the taxonomic composition of bacteriocenosis that settles pores may therefore facilitate the identification of processes occurring in the sediments in the area of underground drainage occurrence, which is particularly important due to the high variability of this phenomenon.
Based on the analysis of the number of indicator bacteria (faecal enterococci and E.coli intestinal bacteria) in seawater and pore waters, the exceedances of the limit values were found only in the summer and autumn seasons. In pore water not affected by drainage, the number of indicator bacteria was similar to or lower than that in seawater. However, the lowest values were recorded in pore water under the influence of drainage. This might be due to dilution of seawater with groundwater, changes in environmental conditions (salinity, Eh, pH, nutrient concentration, oxygen availability) or periodic presence of inhibitors (CH4, H2S). The influence of allochthonous antagonistic microflora, which may affect the partial elimination of microbial contamination of faecal origin, is also not without significance.
In the experimental part of the research, it was observed that the presence of organic substances significantly increased the lifespan of faecal bacteria, both in seawater and in porous waters under the influence of drainage. This is a crucial aspect for the sanitary quality of coastal waters. Therefore the study focused in particular on the epidemiological risk and the possibility of drug resistance occurrence in faecal bacteria.
Representatives of faecal bacteria: Enterococcus spp. and Escherichia coli, were isolated from seawater, porous waters and sandy sediments. However, detailed biochemical identification of the strains showed that the used microbiological media were insufficiently selective and e.g. besides enterococci, other bacteria were also found, mainly of the Staphylococcus genus, including Staphylococcus aureus.
Antibiograms made for Enterococcus spp. strains isolated in the area of the study were similar to clinical ones and reflected the consumption of antibiotics in Poland. The observed presence of drug-resistant bacteria could be related to their selective advantage in conditions of exposure to environmental factors (e.g. UV radiation).
The research also has revealed that the complexity of the processes between biotic and abiotic factors in the sandy beach environment and the shallow littoral zone may lead to erroneous conclusions in the short-term surveys. In order to identify and understand these correlations, cyclical and detailed monitoring is necessary.
Summarizing, the results obtained in this study will contribute to a better understanding of the potential human impact on the water resources quality. They are an important contribution to the discussion on the anthropogenic pollutants inflow from various sources, including the increasingly discussed role of groundwater drainage. Results of the survey are also of significant epidemiological importance and fall within the framework of the multi-annual monitoring programme for recreational areas. This programme, according to the requirements of the European Commission, should also take into account the natural environment sector. The issues discussed are the focus of interest of many European programmes and directives, which considered the lack of monitoring data to be the most important gap in the full implementation of tasks related to water protection.