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Effect of Short-Term GSM Radiation at Representative Levels in Society on a Biological Model: The Ant Myrmica sabuleti
Abstract
Well-controlled electromagnetic exposure conditions were set up at a representative societal GSM radiation intensity level, 1.5 V/m, which is the legally allowed level in Brussels. Two nests of the ant species Myrmica sabuleti were repeatedly irradiated during 10 min. before their behavior was observed, based on the analysis of the ant trajectories. Under these exposure conditions, behavioral effects were detected. The ants’ locomotion slightly changed. The ants’ orientation towards their attractive alarm pheromone statistically became of lower quality. The ants still presented their trail following behavior but less efficiently. In this controversial issue, ants could be considered as possible bioindicators.
... RF heating of insects has been reviewed in [10][11][12]. Studies not necessarily aimed at investigating the thermal effects of EMF exposure exist as well [13][14][15][16][17][18]. [19] suggests that EM radiation of mobile antennas affects the abundance and composition of wild pollinators. ...
This paper presents a study of electromagnetic field (EMF) exposure of a honey bee at radio frequencies from 2.5 GHz to 100 GHz using numerical simulations based on the finite-difference time-domain (FDTD) technique. The main goal of our study was to estimate the Specific Absorption Rate (SAR) in honey bee tissues as a dosimetric aid to predict the possible adverse effects caused by EMF energy absorption. In this study, the insect is modeled as a 3-tissue heterogeneous dielectric object, and the Sim4Life EM-FDTD solver was used for EM simulations. The whole-body and brain tissue-averaged SAR values of a honeybee were determined for all considered frequencies and normalized on 1 mW/cm2 incident plane-wave power density. The obtained SAR values were also averaged over a volume of 1 milligram of tissue. The results showed that EMF energy absorption in insects depends on E-field polarization, frequency, and the insect's body peculiarities.
... In addition, some reviews have focused on studies that investigate different physical environments, such as temperature, radiofrequency radiation, and MF exposure of insects, or prepared pesticides in magnetic solutions, or exposed compounds to an MF [Vijver, 2014;Kandil et al., 2018]. Direct exposure of insects to MF in the laboratory resulted in strong biological effects [Cammaerts et al., 2014;Kandil and Hessein, 2019]. Górski et al. [2009] reported that exposure of pesticides to MF could result in high mortality rates in the two-spotted spider mite, Tetranychus urticae. ...
This study illustrates the effect of magnetic field (MF) on the toxicity of two insecticides, emamectin benzoate (Emazoate 2.15% EC) and spinosad (SpinTor 24% SC), and determines their adverse effects on the bollworm (Earias insulana) through various biological and biochemical assays. The investigation indicated that exposure to the insecticides in a MF of 180 mT resulted in stronger toxicity, with LC50 values of 0.162, 1.211, and 1.770 ppm, respectively. In addition, the results showed that magnetized insecticides significantly increased in the duration of the total immature stages (larvae and/or pupae) 32.1 and 36.6 days, compared with 27.9 and 30.5 days, respectively, in the nonmagnetized insecticides, while untreated check was 21 days. Also, the magnetized insecticides reduced the percentage of adult emergence, and increased deformations in the larval and pupal stages. Furthermore, sex ratio was greatly affected by exposure to both insecticides in conjunction with the MF. Exposure of the larvae of E insulana to magnetized insecticides can bring about malfunction in some biochemical process and significantly decreased the invertase activity, and decreased the total protein and carbohydrates. In contrast, it can increase amylase compared with nonmagnetized insecticides and untreated controls. Results concluded that the two insecticides’ MF affected growth, survival time, and biological and biochemical parameters of E. insulana.
... In research into the biological effects of EMF, it has been known since the 1960s that many species are sensitive to low-level energy exposures. Numerous laboratory and field studies have noted heightened sensitivity and adverse effects in birds [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32]; mammals (cows and bats [33][34][35][36][37][38]); insects [39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54] bacteria/protozoa [55][56][57][58][59][60][61]; amphibians [62][63][64][65][66][67]; fish and turtles [68][69][70][71][72][73][74][75][76][77][78][79][80][81][82]; and in trees and plants [83][84][85], among many others. ...
Ambient levels of electromagnetic fields (EMF) have risen sharply in the last 80 years, creating a novel energetic exposure that previously did not exist. Most recent decades have seen exponential increases in nearly all environments, including rural/remote areas and lower atmospheric regions. Because of unique physiologies, some species of flora and fauna are sensitive to exogenous EMF in ways that may surpass human reactivity. There is limited, but comprehensive, baseline data in the U.S. from the 1980s against which to compare significant new surveys from different countries. This now provides broader and more precise data on potential transient and chronic exposures to wildlife and habitats. Biological effects have been seen broadly across all taxa and frequencies at vanishingly low intensities comparable to today’s ambient exposures. Broad wildlife effects have been seen on orientation and migration, food finding, reproduction, mating, nest and den building, territorial maintenance and defense, and longevity and survivorship. Cyto- and geno-toxic effects have been observed. The above issues are explored in three consecutive parts: Part 1 questions today’s ambient EMF capabilities to adversely affect wildlife, with more urgency regarding 5G technologies. Part 2 explores natural and man-made fields, animal magnetoreception mechanisms, and pertinent studies to all wildlife kingdoms. Part 3 examines current exposure standards, applicable laws, and future directions. It is time to recognize ambient EMF as a novel form of pollution and develop rules at regulatory agencies that designate air as ‘habitat’ so EMF can be regulated like other pollutants. Wildlife loss is often unseen and undocumented until tipping points are reached. Long-term chronic low-level EMF exposure standards, which do not now exist, should be set accordingly for wildlife, and environmental laws should be strictly enforced.
... Also, there is no generally accepted scientific consensus on these health issues. A few effects that have been suggested are the possible interference with the brain's normal functioning [1] by inhibiting the release of melatonin and other endocrine secretions needed to replenish the immune system [2], changed glucose metabolism in the brain [3], adverse effect on the memory of rats [4], change in orientation by ants [5], impacts on biosystems and ecosystems [6], and the fact that careless building design may contribute to the so-called sick building syndrome (SBS) [7]. ...
The electromagnetic shielding effect of common building materials is reviewed in order to provide baseline data for computer simulations. The first part of this paper presents the “raw” electromagnetic properties of these materials, like brick, concrete, wood, glass, and a complex material like reinforced concrete with different reinforcement square grid sizes. The second part of the paper presents the effect of the shape of a room made from these materials and the effect of the rounding of corners and window edges on the in-house electromagnetic fields. Simulations are performed with a state-of-the-art computational tool: CST Microwave Studio. It is demonstrated that, apart from slightly focusing the electromagnetic waves to the centre of the room, rounding has a very small effect on the electric and magnetic fields. The last part presents comparative data about different building materials regarding their attenuation and electromagnetic penetration. At the end a carbon foil based shielding technique is described, and the importance of and solutions for window shielding are presented.
... The geomagnetic field reception in American cockroach is sensitive to weak radio frequency field causing a disruptive effect (Vacha et al., 2009), so these authors suggest that electromagnetic smog will have to be taken more seriously in animal magnetoreception experiments. In an experimentally-generated electromagnetic field of about 1 V/m with a realistic (and even lower) power intensity similar to those surrounding communication masts, the results and observations suggest that GSM (Global System for Mobile communications) 900 MHz radiation might have a severe impact on the nerve cells of exposed ants, especially affecting the visual and olfactory memory, causing the loss of their ability to use visual cues and suggesting that electromagnetic radiation may have an impact on the orientation behaviour and navigation of animals that use magnetic fields to find their way (Cammaerts et al., 2012(Cammaerts et al., , 2014. Honeybees are sensitive to pulsed electromagnetic fields generated by mobile phones and observable changes in the bee behaviour could be one explanation for the loss of colonies (Favre, 2011). ...
The rate of scientific activity regarding the effects of anthropogenic electromagnetic radiation in the radiofrequency (RF) range on animals and plants has been small despite the fact that this topic is relevant to the fields of experimental biology, ecology and conservation due to its remarkable expansion over the past 20years. Current evidence indicates that exposure at levels that are found in the environment (in urban areas and near base stations) may particularly alter the receptor organs to orient in the magnetic field of the earth. These results could have important implications for migratory birds and insects, especially in urban areas, but could also apply to birds and insects in natural and protected areas where there are powerful base station emitters of radiofrequencies. Therefore, more research on the effects of electromagnetic radiation in nature is needed to investigate this emerging threat.
Copyright © 2015 Elsevier B.V. All rights reserved.
Efforts to increase the quality of this environment have been going on since the beginning of the concern for shelter in the history of humanity. The main targets of the user, designers, practitioners, and decision-makers are to carry the quality of life, health, safety, and welfare of individuals and other living things to the next level. Problems have been identified to increase the function and quality of the space, spatial requirements have been determined, and researched, and some design factors have been determined to reach the most appropriate solution. The researchers focused on the proposition that the effectiveness of these factors before and during the design process would increase the quality of the space. However, since research generally focuses on indoor quality, the outdoor quality factors that are part of urban design are not clear. This work, was designed to evaluate the usability of these factors, which were determined and measured especially to deal with the interior, in designs at larger scales (street, neighborhood, city…) and to improve the existing. In designs outside the spatial scale, where almost all factors can be controlled by the relevant stakeholders of the process, the subject is approached through changeable and unchangeable parameters and their balancing. Studies on the factors determined during the process were compiled and their effects on different scales were evaluated as a result of these compilations. As a result of the evaluation, some suggestions were given. In the process of redesigning the space, selecting feasible suggestions, and incorporating them into the creation of the future physical space, using the suggestions as urban rehabilitation tools, and considering these interventions to be flexible and suitable for various factors are of great importance for the psychology of the users.
There is enough evidence to indicate we may be damaging non-human species at ecosystem and biosphere levels across all taxa from rising background levels of anthropogenic non-ionizing electromagnetic fields (EMF) from 0 Hz to 300 GHz. The focus of this Perspective paper is on the unique physiology of non-human species, their extraordinary sensitivity to both natural and anthropogenic EMF, and the likelihood that artificial EMF in the static, extremely low frequency (ELF) and radiofrequency (RF) ranges of the non-ionizing electromagnetic spectrum are capable at very low intensities of adversely affecting both fauna and flora in all species studied. Any existing exposure standards are for humans only; wildlife is unprotected, including within the safety margins of existing guidelines, which are inappropriate for trans-species sensitivities and different non-human physiology. Mechanistic, genotoxic, and potential ecosystem effects are discussed.
Ambient levels of nonionizing electromagnetic fields (EMF) have risen sharply in the last five decades to become a ubiquitous, continuous, biologically active environmental pollutant, even in rural and remote areas. Many species of flora and fauna, because of unique physiologies and habitats, are sensitive to exogenous EMF in ways that surpass human reactivity. This can lead to complex endogenous reactions that are highly variable, largely unseen, and a possible contributing factor in species extinctions, sometimes localized. Non-human magnetoreception mechanisms are explored. Numerous studies across all frequencies and taxa indicate that current low-level anthropogenic EMF can have myriad adverse and synergistic effects, including on orientation and migration, food finding, reproduction, mating, nest and den building, territorial maintenance and defense, and on vitality, longevity and survivorship itself. Effects have been observed in mammals such as bats, cervids, cetaceans, and pinnipeds among others, and on birds, insects, amphibians, reptiles, microbes and many species of flora. Cyto- and geno-toxic effects have long been observed in laboratory research on animal models that can be extrapolated to wildlife. Unusual multi-system mechanisms can come into play with non-human species — including in aquatic environments — that rely on the Earth’s natural geomagnetic fields for critical life-sustaining information. Part 2 of this 3-part series includes four online supplement tables of effects seen in animals from both ELF and RFR at vanishingly low intensities. Taken as a whole, this indicates enough information to raise concerns about ambient exposures to nonionizing radiation at ecosystem levels. Wildlife loss is often unseen and undocumented until tipping points are reached. It is time to recognize ambient EMF as a novel form of pollution and develop rules at regulatory agencies that designate air as ‘habitat’ so EMF can be regulated like other pollutants. Long-term chronic low-level EMF exposure standards, which do not now exist, should be set accordingly for wildlife, and environmental laws should be strictly enforced — a subject explored in Part 3.
Radiofrequency radiation (RFR), used for wireless communications and “smart” building technologies, including the “Internet of Things,” is increasing rapidly. As both RFR exposures and scientific evidence of harmful effects increase apace, it is timely to heed calls to include low RFR levels as a performance indicator for the health, safety and well-being of occupants and the environment.
Adverse biochemical and biological effects at commonly experienced RFR levels indicate that exposure guidelines for the U.S., Canada and other countries, are inadequate to protect public health and the environment.
Some industry liability insurance providers do not offer coverage against adverse health effects from radiation emitted by wireless technologies, and insurance authorities deem potential liability as “high.” Internationally, governments have enacted laws, and medical and public health authorities have issued recommendations, to reduce and limit exposure to RFR.
There is urgent need to implement strategies for no- or low-RFR emitting technologies, and shielding, in building design and retrofitting. These strategies include installing wired (not wireless) Internet networks, corded rather than cordless phones, and cable or wired connections in building systems (e.g., mechanical, lighting, security). Building science can profit from decades of work to institute performance parameters, operationalizing prudent guidelines and best practices. The goal is to achieve RFR exposures that are ALARA, “As Low As Reasonably Achievable.”
We also challenge the business case of wireless systems, because wired or cabled connections are faster, more reliable and secure, emit substantially less RFR, and consume less energy in a sector with rapidly escalating green-house gas emissions.
Insects are continually exposed to Radio-Frequency (RF) electromagnetic fields at different frequencies. The range of frequencies used for wireless telecommunication systems will increase in the near future from below 6 GHz (2 G, 3 G, 4 G, and WiFi) to frequencies up to 120 GHz (5 G). This paper is the first to report the absorbed RF electromagnetic power in four different types of insects as a function of frequency from 2 GHz to 120 GHz. A set of insect models was obtained using novel Micro-CT (computer tomography) imaging. These models were used for the first time in finite-difference time-domain electromagnetic simulations. All insects showed a dependence of the absorbed power on the frequency. All insects showed a general increase in absorbed RF power at and above 6 GHz, in comparison to the absorbed RF power below 6 GHz. Our simulations showed that a shift of 10% of the incident power density to frequencies above 6 GHz would lead to an increase in absorbed power between 3-370%.
The exponential increase of mobile telephony has led to a pronounced increase in electromagnetic fields in the environment that may affect pollinator communities and threaten pollination as a key ecosystem service. Previous studies conducted on model species under laboratory conditions have shown negative effects of electromagnetic radiation (EMR) on reproductive success, development, and navigation of insects. However, the potential effects that widespread mobile telecommunication antennas have on wild pollinator communities outside the laboratory microcosm are still unknown. Here we studied the effects of EMR from telecommunication antennas on key wild pollinator groups (wild bees, hoverflies, bee flies, remaining flies, beetles, butterflies, and wasps). We measured EMR at 4 distances (50, 100, 200 and 400 m) from 10 antennas (5 on Limnos Island and 5 on Lesvos Island, eastern Mediterranean, Greece), and correlated EMR values with insect abundance and richness (the latter only for wild bees and hoverflies). All pollinator groups except butterflies were affected by EMR. In both islands, beetle, wasp, and hoverfly abundance decreased with EMR, whereas the abundance of underground-nesting wild bees and bee flies unexpectedly increased with EMR. The effect of EMR on the abundance of remaining flies differed between islands. With respect to species richness, EMR only tended to have a negative effect on hoverflies in Limnos. As EMR affected the abundance of several insect guilds negatively, and changed the composition of wild pollinators in natural habitats, it might also have additional ecological and economic impacts on the maintenance of wild plant diversity, crop production and human welfare.
Fire is an important and frequent disturbance in Mediterranean-type ecosystems affecting the structure and dynamics of bee communities. We explored the effect of fire history on taxonomic and functional composition of wild bees on Rhodes Island, Greece, using UV-bright pan trap sampling. We carried out a fourth-corner analysis based on Generalized Linear Models to identify significant interactions between functional traits (nesting preferences, trophic specialization and body size) and fire history, defined by fire frequency and post-fire age of sites. Our analysis showed that species diversity and abundance at various taxonomic levels did not display significant differences among different fire history regimes (twice-burnt, recently-burnt, old-burnt and unburnt). The effect of fire was detected when focusing on functional traits rather than species. Ground nesting bees were positively associated with recently-burnt sites, which provide bare soil and therefore nesting sites for this group of bees. Polylectic bees were positively associated with twice-burnt sites, indicating the dominance of generalist bee species. Larger bees were positively associated with post-fire age, probably due to the larger foraging range of larger bees that enables a more efficient exploitation of aggregated resources. Our results showed that responses of wild bee species to fire are driven by nesting preference, trophic specialization and body size.
Gametogenesis in all animals, seems to be a much more sensitive developmental stage than later stages of their lives. In Drosophila melanogaster, gametogenesis -especially oogenesis - is shown to be a very sensitive biological phase, in response to exogenous stress factors like radiation - including electromagnetic fields (EMFs) - chemicals, elevated or low temperature, food deprivation, etc. Stress-induced cell death during oogenesis is already studied for a variety of stress factors. The high sensitivity of oogenesis, in combination with the fact that particularly in Drosophila it is very well studied, makes this animal a valuable tool for the study of the biological activity of different environmental agents. The developmental stages of oogenesis, embryonic, and post-embryonic development in Drosophila exhibit a good timing under certain environmental conditions, and this has led us to the design of experimental protocols based on the assessment of the reproductive capacity, by the number of the first filial generation (F1) pupae, reflecting the effect of the different exogenous factors on gametogenesis and, in turn, on oviposition. Due to the existence of special checkpoints during oogenesis (most sensitive developmental stages), all the abnormally developing eggs are properly eliminated by stress-induced cell death on the nurse and follicle cells during early and mid-oogenesis leading to the destruction of the whole egg-chamber. Thus, usually, environmental factors do not have an effect on the offspring's genome and morphology but rather on the number of laid eggs (oviposition) and, consequently, on the number of developing post-embryos (larvae and pupae). For this, the number of F1 pupae, under certain conditions, may reflect biological changes due to exogenous factors. The situation may be more complicated when the exogenous stress factor is microwave radiation. This kind of man-made radiation is found to damage DNA not only in the nurse and follicle cells during oogenesis as by other previously known stress factors, but also in the oocyte from which the progeny organism will be developed after fertilization. DNA damage in the oocyte may result in inherited mutations transferred to the next generations and for this reason the biological changes due to microwave radiation may not be restricted only to changes in the reproductive capacity. In this chapter, Drosophila gametogenesis, embryonic, post-embryonic development and life-cycle are briefly described. The chapter then focuses on stress-induced cell death during oogenesis, and discusses a basic experimental protocol regarding the effect of environmental stress factors on reproductive capacity, based on the increased sensitivity of Drosophila gametogenesis and the good timing of the embryonic and post-embryonic developmental phases.
This paper summarizes the effect of radio-frequency electromagnetic field (RF-EMF) from cell towers and wireless devices on the biosphere. Based on current available literature, it is justified to conclude that RF-EMF radiation expo- sure can change neurotransmitter functions, blood-brain barrier, morphology, electrophysiology, cellular metabolism, calcium efflux, and gene and protein expression in certain types of cells even at lower intensities. The biological consequences of such changes remain unclear. Short-term studies on the impacts of RF-EMF on frogs, honey bees, house sparrows, bats, and even humans are scarce and long-term studies are non-existent in India. Identification of the frequency, intensity, and duration of non-ionizing electromagnetic fields causing damage to the biosystem and ecosystem would evolve strategies for mitigation and would enable the proper use of wireless technologies to enjoy its immense benefits, while ensuring one’s health and that of the environment.
The direct targets of extremely low and microwave frequency range electromagnetic fields (EMFs) in producing non-thermal effects have not been clearly established. However, studies in the literature, reviewed here, provide substantial support for such direct targets. Twenty-three studies have shown that voltage-gated calcium channels (VGCCs) produce these and other EMF effects, such that the L-type or other VGCC blockers block or greatly lower diverse EMF effects. Furthermore, the voltage-gated properties of these channels may provide biophysically plausible mechanisms for EMF biological effects. Downstream responses of such EMF exposures may be mediated through Ca(2+) /calmodulin stimulation of nitric oxide synthesis. Potentially, physiological/therapeutic responses may be largely as a result of nitric oxide-cGMP-protein kinase G pathway stimulation. A well-studied example of such an apparent therapeutic response, EMF stimulation of bone growth, appears to work along this pathway. However, pathophysiological responses to EMFs may be as a result of nitric oxide-peroxynitrite-oxidative stress pathway of action. A single such well-documented example, EMF induction of DNA single-strand breaks in cells, as measured by alkaline comet assays, is reviewed here. Such single-strand breaks are known to be produced through the action of this pathway. Data on the mechanism of EMF induction of such breaks are limited; what data are available support this proposed mechanism. Other Ca(2+) -mediated regulatory changes, independent of nitric oxide, may also have roles. This article reviews, then, a substantially supported set of targets, VGCCs, whose stimulation produces non-thermal EMF responses by humans/higher animals with downstream effects involving Ca(2+) /calmodulin-dependent nitric oxide increases, which may explain therapeutic and pathophysiological effects.
Flowers and Bees Have “Sparks”
Plants and their pollinators have very intimate interactions, as emphasized by the many classic cases of coevolution among species of each. Such close relationships require signaling between plant and pollinator. Coordination between plant signals and pollinator perception has been shown to exist in flower color, shape, and odor. Clarke et al. (p. 66 , published online 21 February) report the potential for a distinct mode of plant–pollinator communication, electric fields. Natural floral electric fields, which are impacted by visits from naturally charged bees, were easily discriminated by bees, based on their level, pattern, and structure, and improved the rate at which bees remembered the location of a nectar reward.
The usage of mobile phone increases globally. However, there is still a paucity of data about the impact of electromagnetic fields (EMF) on human health. This study investigated whether EMF radiation would alter the biology of glial cells and act as a tumor-promoting agent. We exposed rat astrocytes and C6 glioma cells to 1950-MHz TD-SCDMA for 12, 24 and 48 h respectively, and found that EMF exposure had differential effects on rat astroctyes and C6 glioma cells. A 48 h of exposure damaged the mitochondria and induced significant apoptosis of astrocytes. Moreover, caspase-3, a hallmark of apoptosis, was highlighted in astrocytes after 48 h of EMF exposure, accompanied by a significantly increased expression of bax and reduced level of bcl-2. The tumorigenicity assays demonstrated that astrocytes did not form tumors in both control and exposure groups. In contrast, the unexposed and exposed C6 glioma cells show no significant differences in both biological feature and tumor formation ability. Therefore, our results implied that exposure to the EMF of 1950-MHz TD-SCDMA may not promote the tumor formation, but continuous exposure damaged the mitochondria of astrocytes and induce apoptosis through a caspase-3-dependent pathway with the involvement of bax and bcl-2.
The worldwide maintenance of the honeybee has major ecological, economic, and political implications. In the present study,
electromagnetic waves originating from mobile phones were tested for potential effects on honeybee behavior. Mobile phone
handsets were placed in the close vicinity of honeybees. The sound made by the bees was recorded and analyzed. The audiograms
and spectrograms revealed that active mobile phone handsets have a dramatic impact on the behavior of the bees, namely by
inducing the worker piping signal. In natural conditions, worker piping either announces the swarming process of the bee colony
or is a signal of a disturbed bee colony.
Keywordsworker bee–acoustic communication–mobile phone handset–worker piping–induction
In the present study we examine the effect of microwave exposure to a GSM-like frequency of 970 MHz pulsed waves on the memory in rats by means of an object recognition task. Two populations of Wistar albino rats, each composed of 2 subgroups of 32 rats, have been exposed to low level radiofrequent radiation for 2 hours/day during 2 and 15 months, respectively. The rats that have been exposed for 2 months show normal exploratory behaviour. The animals that have been exposed for 15 months show derogatory behaviour. They do not make the distinction between a familiar and an unfamiliar object
The kinetics of the acquisition and loss of the use of olfactory and visual cues were previously obtained in six experimental colonies of the ant Myrmica sabuleti meinert 1861, under normal conditions. In the present work, the same experiments were conducted on six other naive identical colonies of M. sabuleti, under electromagnetic radiation similar to those surrounding GSM and communication masts. In this situation, no association between food and either olfactory or visual cues occurred. After a recovery period, the ants were able to make such an association but never reached the expected score. Such ants having acquired a weaker olfactory or visual score and still undergoing olfactory or visual training were again submitted to electromagnetic waves. Not only did they lose all that they had memorized, but also they lost it in a few hours instead of in a few days (as under normal conditions when no longer trained). They kept no visual memory at all (instead of keeping 10% of it as they normally do). The impact of GSM 900 MHz radiation was greater on the visual memory than on the olfactory one. These communication waves may have such a disastrous impact on a wide range of insects using olfactory and/or visual memory, i.e., on bees.
The protozoan Paramecium caudatum was examined under normal conditions versus aside a switched-on GSM telephone (900 MHz; 2 Watts). Exposed individuals moved more slowly and more sinuously than usual. Their physiology was affected: they became broader, their cytopharynx appeared broader, their pulse vesicles had difficult in expelling their content outside the cell, their cilia less efficiently moved, and trichocysts became more visible. All these effects might result from some bad functioning or damage of the cellular membrane. The first target of communication electromagnetic waves might thus be the cellular membrane.
Numerous incidents of aspen decline have been recorded in North America over the past half century, and incidents of very rapid mortality of aspen clones have been observed in Colorado since 2004. The radio frequency (RF) environment of the earth has undergone major changes in the past two centuries due to the development and use of electricity in power and communications applications, and the anthropogenic RF background continues to increase in intensity and complexity. This study suggests that the RF background may have strong adverse effects on growth rate and fall anthocyanin production in aspen, and may be an underlying factor in aspen decline.
Behavioural experiments for magnetoreception in eusocial insects in the last decade are reviewed. Ants and bees use the geomagnetic field to orient and navigate in areas around their nests and along migratory paths. Bees show sensitivity to small changes in magnetic fields in conditioning experiments and when exiting the hive. For the first time, the magnetic properties of the nanoparticles found in eusocial insects, obtained by magnetic techniques and electron microscopy, are reviewed. Different magnetic oxide nanoparticles, ranging from superparamagnetic to multi-domain particles, were observed in all body parts, but greater relative concentrations in the abdomens and antennae of honeybees and ants have focused attention on these segments. Theoretical models for how these specific magnetosensory apparatuses function have been proposed. Neuron-rich ant antennae may be the most amenable to discovering a magnetosensor that will greatly assist research into higher order processing of magnetic information. The ferromagnetic hypothesis is believed to apply to eusocial insects, but interest in a light-sensitive mechanism is growing. The diversity of compass mechanisms in animals suggests that multiple compasses may function in insect orientation and navigation. The search for magnetic compasses will continue even after a magnetosensor is discovered in eusocial insects.
Large numbers of bats are killed by collisions with wind turbines and there is at present no accepted method of reducing or preventing this mortality. Following our demonstration that bat activity is reduced in the vicinity of large air traffic control and weather radars, we tested the hypothesis that an electromagnetic signal from a small portable radar can act as a deterrent to foraging bats. From June to September 2007 bat activity was compared at 20 foraging sites in northeast Scotland during experimental trials (radar switched on) and control trials (no radar signal). Starting 45 minutes after sunset, bat activity was recorded for a period of 30 minutes during each trial and the order of trials were alternated between nights. From July to September 2008 aerial insects at 16 of these sites were sampled using two miniature light-suction traps. At each site one of the traps was exposed to a radar signal and the other functioned as a control. Bat activity and foraging effort per unit time were significantly reduced during experimental trials when the radar antenna was fixed to produce a unidirectional signal therefore maximising exposure of foraging bats to the radar beam. However, although bat activity was significantly reduced during such trials, the radar had no significant effect on the abundance of insects captured by the traps.
The possible risks of radio-frequency electromagnetic fields for the human body is a growing concern for our society. We have previously shown that weak pulsed microwaves give rise to a significant leakage of albumin through the blood-brain barrier. In this study we investigated whether a pathologic leakage across the blood-brain barrier might be combined with damage to the neurons. Three groups each of eight rats were exposed for 2 hr to Global System for Mobile Communications (GSM) mobile phone electromagnetic fields of different strengths. We found highly significant (p< 0.002) evidence for neuronal damage in the cortex, hippocampus, and basal ganglia in the brains of exposed rats.
A possible effect of long-term exposure to low-intensity electromagnetic radiation from mobile phone (GSM) base stations on the number of House Sparrows during the breeding season was studied in six residential districts in Belgium. We sampled 150 point locations within the 6 areas to examine small-scale geographic variation in the number of House Sparrow males and the strength of electromagnetic radiation from base stations. Spatial variation in the number of House Sparrow males was negatively and highly significantly related to the strength of electric fields from both the 900 and 1800 MHz downlink frequency bands and from the sum of these bands (Chi(2)-tests and AIC-criteria, P<0.001). This negative relationship was highly similar within each of the six study areas, despite differences among areas in both the number of birds and radiation levels. Thus, our data show that fewer House Sparrow males were seen at locations with relatively high electric field strength values of GSM base stations and therefore support the notion that long-term exposure to higher levels of radiation negatively affects the abundance or behavior of House Sparrows in the wild.
Using an especially-designed facility, the Mode Stirred Reverberation Chamber, we exposed tomato plants (Lycopersicon esculentum Mill. VFN8) to low level (900 MHz, 5 V m(-1)) electromagnetic fields for a short period (10 min) and measured changes in abundance of three specific mRNA soon after exposure. Within minutes of electromagnetic stimulation, stress-related mRNA (calmodulin, calcium-dependent protein kinase and proteinase inhibitor) accumulated in a rapid, large and 3-phase manner typical of an environmental stress response. Accumulation of these transcripts into the polysomal RNA also took place (indicating that the encoded proteins were translated) but was delayed (indicating that newly-synthesized mRNA was not immediately recruited into polysomes). Transcript accumulation was maximal at normal Ca(2+) levels and was depressed at higher Ca(2+), especially for those encoding calcium-binding proteins. Removal of Ca(2+) (by addition of chelating agents or Ca(2+) channel blocker) led to total suppression of mRNA accumulation. Finally, 30 min after the electromagnetic treatment, ATP concentration and adenylate energy charge were transiently decreased, while transcript accumulation was totally prevented by application of the uncoupling reagent, CCCP. These responses occur very soon after exposure, strongly suggesting that they are the direct consequence of application of radio-frequency fields and their similarities to wound responses strongly suggests that this radiation is perceived by plants as an injurious stimulus.
Medically unsubstantiated 'intolerances' to foods, chemicals and environmental toxins are common and are frequently discussed in the media. Idiopathic environmental intolerance attributed to electromagnetic fields (IEI-EMF) is one such condition and is characterized by symptoms that are attributed to exposure to electromagnetic fields (EMF). In this experiment, we tested whether media reports promote the development of this condition.
Participants (N=147) were randomly assigned to watch a television report about the adverse health effects of WiFi (n=76) or a control film (n=71). After watching their film, participants received a sham exposure to a WiFi signal (15min). The principal outcome measure was symptom reports following the sham exposure. Secondary outcomes included worries about the health effects of EMF, attributing symptoms to the sham exposure and increases in perceived sensitivity to EMF.
82 (54%) of the 147 participants reported symptoms which they attributed to the sham exposure. The experimental film increased: EMF related worries (β=0.19; P=.019); post sham exposure symptoms among participants with high pre-existing anxiety (β=0.22; P=.008); the likelihood of symptoms being attributed to the sham exposure among people with high anxiety (β=.31; P=.001); and the likelihood of people who attributed their symptoms to the sham exposure believing themselves to be sensitive to EMF (β=0.16; P=.049).
Media reports about the adverse effects of supposedly hazardous substances can increase the likelihood of experiencing symptoms following sham exposure and developing an apparent sensitivity to it. Greater engagement between journalists and scientists is required to counter these negative effects.
We used the ant species Myrmica sabuleti as a model to study the impact of electromagnetic waves on social insects' response to their pheromones and their food collection. We quantified M. sabuleti workers' response to their trail, area marking and alarm pheromone under normal conditions. Then, we quantified the same responses while under the influence of electromagnetic waves. Under such an influence, ants followed trails for only short distances, no longer arrived at marked areas and no longer orientated themselves to a source of alarm pheromone. Also when exposed to electromagnetic waves, ants became unable to return to their nest and recruit congeners; therefore, the number of ants collecting food increases only slightly and slowly. After 180 h of exposure, their colonies deteriorated. Electromagnetic radiation obviously affects social insects' behavior and physiology.
Increase in the usage of electronic gadgets has led to electropollution of the environment. Honeybee behaviour and biology has been affected by electrosmog since these insects have magnetite in their bodies
which helps them in navigation. There are reports of sudden disappearance of bee populations from honeybee colonies. The reason is still not clear. We have compared the performance of honeybees in cellphone radiation exposed and unexposed colonies. A significant (p < 0.05) decline in colony strength and in the egg laying rate of the queen was observed. The behaviour of exposed foragers was negatively influenced by the exposure, there was neither honey nor pollen in the colony at the end of the experiment.
Leafcutter ants, Atta colombica, forage over 250 m in structurally complex, Neotropical rainforests that occlude sun or polarized light cues. Night foraging makes the use of celestial cues and landmarks all the more difficult. We investigated the directional cues used by leafcutter ants to orient homeward by experimentally reversing the polarity of the local magnetic field and by experimentally subjecting the ants to a strong magnetic pulse to disrupt a magnetic compass. In both experiments, we transferred homeward-bound ants from a foraging trail to a table in a chamber that occluded landmark and celestial cues. In both experiments, control ants showed path integration and walked directly towards the nest. In the reversed field, one-half of the experimental ants oriented according to the reversed field (geographically 180° opposite to the nest's direction), indicating that they used a magnetic compass to update their positional reference derived from path integration. The other half walked towards the nest, suggesting that they may have used an egocentric reference to measure their rotation when displaced, although other explanations have not been entirely excluded. With application of a very brief, but strong, magnetic pulse, experimental ants oriented randomly. We conclude that the leafcutter ants use the earth's magnetic field as a reference by which to orient when path-integrating towards home.
The use of mobile phones is now widespread. A great debate exists about the possible damage that the radiofrequency electromagnetic radiation (RF-EMR) emitted by mobile phones exerts on different organs and apparatuses. The aim of this article was to review the existing literature exploring the effects of RF-EMR on the male reproductive function in experimental animals and humans. Studies have been conducted in rats, mice, and rabbits using a similar design based upon mobile phone RF exposure for variable lengths of time. Together, the results of these studies have shown that RF-EMR decreases sperm count and motility and increases oxidative stress. In humans, 2 different experimental approaches have been followed: one has explored the effects of RF-EMR directly on spermatozoa and the other has evaluated the sperm parameters in men using or not using mobile phones. The results showed that human spermatozoa exposed to RF-EMR have decreased motility, morphometric abnormalities, and increased oxidative stress, whereas men using mobile phones have decreased sperm concentration, decreased motility (particularly rapid progressive motility), normal morphology, and decreased viability. These abnormalities seem to be directly related to the duration of mobile phone use.
The dramatic increase in use of cellular telephones has generated concern about possible negative effects of radiofrequency signals delivered to the brain. However, whether acute cell phone exposure affects the human brain is unclear.
To evaluate if acute cell phone exposure affects brain glucose metabolism, a marker of brain activity.
Randomized crossover study conducted between January 1 and December 31, 2009, at a single US laboratory among 47 healthy participants recruited from the community. Cell phones were placed on the left and right ears and positron emission tomography with ((18)F)fluorodeoxyglucose injection was used to measure brain glucose metabolism twice, once with the right cell phone activated (sound muted) for 50 minutes ("on" condition) and once with both cell phones deactivated ("off" condition). Statistical parametric mapping was used to compare metabolism between on and off conditions using paired t tests, and Pearson linear correlations were used to verify the association of metabolism and estimated amplitude of radiofrequency-modulated electromagnetic waves emitted by the cell phone. Clusters with at least 1000 voxels (volume >8 cm(3)) and P < .05 (corrected for multiple comparisons) were considered significant.
Brain glucose metabolism computed as absolute metabolism (μmol/100 g per minute) and as normalized metabolism (region/whole brain).
Whole-brain metabolism did not differ between on and off conditions. In contrast, metabolism in the region closest to the antenna (orbitofrontal cortex and temporal pole) was significantly higher for on than off conditions (35.7 vs 33.3 μmol/100 g per minute; mean difference, 2.4 [95% confidence interval, 0.67-4.2]; P = .004). The increases were significantly correlated with the estimated electromagnetic field amplitudes both for absolute metabolism (R = 0.95, P < .001) and normalized metabolism (R = 0.89; P < .001).
In healthy participants and compared with no exposure, 50-minute cell phone exposure was associated with increased brain glucose metabolism in the region closest to the antenna. This finding is of unknown clinical significance.
It is important to determine the possible effects of exposure to radiofrequency (RF) radiation on the genetic material of cells since damage to the DNA of somatic cells may be linked to cancer development or cell death and damage to germ cells may lead to genetic damage in next and subsequent generations. The objective of this study was to investigate whether exposure to radiofrequency radiation similar to that emitted by mobile phones of second-generation standard Global System for Mobile Communication (GSM) induces genotoxic effects in cultured human cells. The cytogenetic effects of GSM-900 MHz (GSM-900) RF radiation were investigated using R-banded karyotyping after in vitro exposure of human cells (amniotic cells) for 24 h. The average specific absorption rate (SAR) was 0.25 W/kg. The exposures were carried out in wire-patch cells (WPCs) under strictly controlled conditions of temperature. The genotoxic effect was assessed immediately or 24 h after exposure using four different samples. One hundred metaphase cells were analyzed per assay. Positive controls were provided by using bleomycin. We found no direct cytogenetic effects of GSM-900 either 0 h or 24 h after exposure. To the best of our knowledge, our work is the first to study genotoxicity using complete R-banded karyotyping, which allows visualizing all the chromosomal rearrangements, either numerical or structural.
Studies were performed to determine the effects of microwave on synaptic vesicles and the expression of synaptic vesicular associated proteins including synapsin I, VAMP-2, syntaxin, and synaptophysin. 25 Wistar rats were exposed to microwave which the average power density was 30 mW/cm(2), and whole body average specific absorption rate was 14.1 W/kg for 5 min. Synaptosome preparations in the cerebral cortex and hippocampus were obtained by isotonic Percoll/sucrose discontinuous gradients at 6 h, 1, 3, and 7 days after radiation. The expression of synaptic vesicular associated proteins was measured using Western blots and image analysis. The interaction between VAMP-2 and syntaxin was examined by coimmunoprecipitation analysis. Synapsin I in the cerebral cortex were decreased at 3 days (P < 0.01) after radiation and in the hippocampus increased at 1 day (P < 0.01), decreased at 3 days (P < 0.01), increased again at 7 days (P < 0.01) after exposure, compared with the sham-treated controls. Synaptophysin were increased in 1-7 days (P < 0.01) after exposure in the cerebral cortex and hippocampus. VAMP-2 were decreased at 1 and 3 days (P < 0.01) and syntaxin were decreased in 6 h to 3 days (P < 0.01) after radiation in the cerebral cortex and hippocampus. The interactions between VAMP-2 and syntaxin were decreased at 3-7 days (P < 0.01) after radiation in the cerebral cortex and hippocampus, compared with the sham-treated controls. These results suggest that 30 mW/cm(2) (SAR 14.1 W/kg) microwave radiation can result in the perturbation of the synaptic vesicles associated proteins: synapsin I, synaptophysin, VAMP-2, and syntaxin. The perturbation could induce the deposit of synaptic vesicle, which might be relative to the dysfunction of the synaptic transmission, even the cognition deficit.
The exposure to non-thermal microwave electromagnetic field (MW-EMF) at 1.95 MHz, a frequency used in mobile communication, affects the refolding kinetics of eukaryotic proteins (Mancinelli et al., 2004). On these basis we have evaluated the in vivo effect of MW-EMF in human epidermoid cancer KB cells. We have found that MW-EMF induces time-dependent apoptosis (45% after 3 h) that is paralleled by an about 2.5-fold decrease of the expression of ras and Raf-1 and of the activity of ras and Erk-1/2. Although also the expression of Akt was reduced its activity was unchanged likely as a consequence of the increased expression of its upstream activator PI3K. In the same experimental conditions an about 2.5-fold increase of the ubiquitination of ras and Raf-1 was also found and the addition for 12 h of proteasome inhibitor lactacystin at 10 μM caused an accumulation of the ubiquitinated isoforms of ras and Raf-1 and counteracted the effects of MW-EMF on ras and Raf-1 expression suggesting an increased proteasome-dependent degradation induced by MW-EMF. The exposure of KB cells to MW-EMF induced a differential activation of stress-dependent pathway with an increase of JNK-1 activity and HSP70 and 27 expression and with a reduction of p38 kinase activity and HSP90 expression. The overexpression of HSP90 induced by transfection of KB cells with a plasmid encoding for the factor completely antagonized the apoptosis and the inactivation of the ras → Erk-dependent survival signal induced by MW-EMF. Conversely, the inhibition of Erk activity induced by 12 h exposure to 10 mM Mek-1 inhibitor U0126 antagonized the effects induced by HSP90 transfection on apoptosis caused by MW-EMF. In conclusion, these results demonstrate for the first time that MW-EMF induces apoptosis through the inactivation of the ras → Erk survival signaling due to enhanced degradation of ras and Raf-1 determined by decreased expression of HSP90 and the consequent increase of proteasome dependent degradation.
The aim of this study was to investigate microwave (MW) effects on neuronal apoptosis in vitro.
Human neuroblastoma cells SH-SY5Y were exposed to a 900 MHz global system for mobile communication (GSM) or continuous-wave (CW) radiofrequency fields for 24 h in a wire-patch cell. The specific absorption rates (SAR) used were 2 W/kg for CW and 0.25 W/kg average for GSM. During CW exposure, an increase of 2 degrees C was measured, and controls with cells exposed to 39 degrees C were then performed. Apoptosis rate was assessed immediately or 24 h after exposure using three methods: (i) 4',6-diamino-2-phenylindole (DAPI) staining; (ii) flow cytometry using double staining with TdT-mediated dUTP nick-end labeling (TUNEL) and propidium iodide (PI); and (iii) measurement of caspase-3 activity by fluorimetry.
No statistically significant difference in the apoptosis rate was observed between sham and 24 h MW-exposed cells, either GSM-900 at an average SAR of 0.25 W/kg, or CW 900 MHz at a SAR of 2 W/kg, either 0 h or 24 h post-exposure. Furthermore, for CW-exposure, apoptosis rates were comparable between sham-, CW-, 37 degrees C- and 39 degrees C-exposed cells. All three methods used to assess apoptosis were concordant.
These results showed that, under the conditions of the present experiment, MW-exposure (either CW or GSM-900) does not significantly increase the apoptosis rate in the human neuroblastoma cell line SH-SY5Y.
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