Nowadays, for the choice of reproducers the selection pressure is focused mainly on
bulls characteristics, however, little attention is taken to the uterine environment of
female cows. In milk production the reproduction is a fundamental pillar, since in
order to achieve the highest efficiency of the system it is necessary for the cows to
conceive and calve at regular intervals. Uterine diseases, mainly non-specific
infections, are the most common cause of infertility and produce significant economic
losses to the dairy industry. Endometritis is a mucosa uterine inflammation, which in
the absence of clinical signs is defined as subclinical endometritis (ES). The main
characteristic of this entity is the presence of neutrophils in the uterus and in cows
subclinical endometritis can be detected by cytology using the cytobrush technique.
Cytobrush is an operator dependent technique which requires time and technical
skills, for this reason it is not a routine practice in a farm. Cervico-vaginal mucus
(CVM) provides valuable information about the health condition of the female
reproductive tract, therefore, it has great potential as a source of biomarkers for
pathologies of the reproductive system in women and animals. Furthermore, it is
collected easily and quickly, without causing damage to the uterine mucosa and
without special training. The goal of this thesis was to evaluate the physicochemical
characteristics of cervico-vaginal mucus of Holando Argentino dairy cows with and
without subclinical endometritis. The study population consisted of 450 Holando
Argentino cows from farms located in the dairy basin of Santa Fe, Argentina. Cows
were classified into three experimental groups according to discharge type and
cytobrush reading: healthy, subclinical endometritis, and clinical endometritis. The variables analysed in the mucus were: pH, discharge type, macroscopic and
microscopic characteristics, leukocyte esterase enzyme activity (LE), total solids
concentration, proteins and ions (Na+
, K+
, Mg2+), refractive index and water
percentage. Furthermore, the prevalence of the disease was evaluated. As a result, it
was obtained that the prevalence of ES in the dairy basin of Santa Fe was 35%.
Significant differences were found between the behaviour of the entities and the
seasons of the year, being spring the season with the lowest percentage of
endometritis. Regarding the macroscopic analysis of the mucus, no significant
relationship was found between the cloudy aspect and the entity. Differences were
found between the type of background of MCV spreads and uterine status
(p<0.0001), as well as between the percentage of neutrophils in cytobrush in
samples with a clean and dirty background (p<0.0001). The background study as a
diagnostic technique yielded 58% sensitivity and 85% specificity. 86% of the MCV
samples were positive for the LE test, statistically significant differences were found
in the LE reading between the S and ES groups (p<0.0001). The leukocyte esterase
test revealed 86% sensitivity and 95% specificity for the diagnosis of ES. Regarding
the pH, differences were obtained between the groups (p=0.0026), being more
alkaline in MCV of females that had ES. No differences were found between the
groups in the total solids concentration, total proteins concentration, Mg2+
, Na+
, water
percentage and refractivity values (p>0.05). Differences were found in the K+
concentration between the groups (p=0.01), as well as in the Na
+
/K+ relationship
(p=0.003). In conclusion, no association was found between discharge type and ES.
An association was found between the percentage of neutrophils in cytobrush and
the type of MCV spread background. The physicochemical characteristics that were
modified in ES cow MCV were: pH, K+ concentration and LE activity. The total solids
concentration, total proteins concentration, Mg2+
, Na+
, water percentage, and
refractivity values did not change. The leukocyte esterase test that revealed high
sensitivity and specificity for the diagnosis of subclinical endometritis in mucus
samples.