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Selective antibacterial effects of mixed ZnMgO nanoparticles

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Jasmina Vidic at Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Jasmina Vidic
  • Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Slavica Stankic at French National Centre for Scientific Research
Slavica Stankic
Francia Haque
Francia Haque
Francia Haque
  • This person is not on ResearchGate, or hasn't claimed this research yet.
Danica Ciric at Institut Pasteur
Danica Ciric
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Antibiotic resistance has impelled the research for new agents that can inhibit bacterial growth without showing cytotoxic effects on humans and other species. We describe the synthesis and physicochemical characterization of nanostructured ZnMgO whose antibacterial activity was compared to its pure nano-ZnO and nano-MgO counterparts. Among the three oxides, ZnO nanocrystals-with the length of tetrapod legs about 100 nm and the diameter about 10 nm-were found to be the most effective antibacterial agents since both Gram-positive (B. subtilis) and Gram-negative (E. coli) bacteria were completely eradicated at concentration of 1 mg/mL. MgO nanocubes (the mean cube size ~50 nm) only partially inhibited bacterial growth, whereas ZnMgO nanoparticles (sizes corresponding to pure particles) revealed high specific antibacterial activity to Gram-positive bacteria at this concentration. Transmission electron microscopy analysis showed that B. subtilis cells were damaged after contact with nano-ZnMgO, causing cell contents to leak out. Our preliminary toxicological study pointed out that nano-ZnO is toxic when applied to human HeLa cells, while nano-MgO and the mixed oxide did not induce any cell damage. Overall, our results suggested that nanostructured ZnMgO, may reconcile efficient antibacterial efficiency while being a safe new therapeutic for bacterial infections.
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... Additionally, the contact of the bacterial cell wall with the mixture may result in the deformation of a cell wall structure (Karthik et al., 2019). Moreover, Vidic et al. (2013) and Huang et al. (2005) showed that MgO NPs inhibited bacterial growth. Furthermore, MgO-NPs showed outstanding bactericidal action against Proteus vulgaris, Pseudomonas aurigenosa, and Escherichia coli, according to research by Faisal et al. (2020). ...
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... Our results are consistent with the findings of Ref. [91], where Pl-ZnO NPs biosynthesized using Bauhini tomentosa leaf extract displayed antibacterial effects across all tested bacterial strains. The bactericidal effect of Pl-ZnO NPs was notably higher for Gram-negative bacteria than for Gram-positive bacteria, which was influenced by structural composition differences [92]. The reported zones of inhibition exhibited notable diameters for P. aeruginosa (20.3 mm), E. coli (19.8 mm), B. subtilis (8.1 mm), and S. aureus (10.7 mm). ...
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... A broad peak at 253 nm and small intensity of the peak at 320 nm consistent with the Cu-O bond in CuONPs and π-π* transition of benzoyl in the extract are shown in fig. 1A (c) 46 . CuO/ZnO nanocomposite showed that a broad peak with low intensity at 334 nm was effectively coated on ZnONPs 9,14,23 . ...
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  • RES J CHEM ENVIRON
We have focused on the green synthesis of copper oxide nanoparticles (CuONPs) and zinc oxide nanoparticles (ZnONPs) and CuO coated with ZnO nanocomposite (CuO/ZnO) by using leaf extract of Cimpobogan citrates (CC) as reducing and stabilizing agent. Copper sulfate pentahydrate (CuSO4.5H2O) and zinc acetate dihydrate (Zn(CH3COO)2.2H2O) are the forerunners in the hydrothermal synthesis. The prepared ZnONPs, CuONPs and CuO/ZnO nanocomposite were characterized using various instrument techniques such as Fourier Transform-Infrared spectroscopy (FT-IR), UV-visible spectroscopy (UV-Vis), X-ray diffraction pattern (XRD), Scanning electron microscope (SEM), Energy dispersive X-ray analysis (EDAX) and High Resolution-Transmission Electron microscope (HR-TEM). The results showed that the average size of CuO/ZnO nanocomposite was 20-50 nm. The Tauc plot of CuO/ZnO nanocomposite shows low band gap (1.56ev). Under visible light illumination, methylene blue (MB) was degraded photocatalytically using ZnONPs, CuONPs and CuO/ZnO nanocomposite which was then examined using UV-visible spectroscopy. In comparison to ZnONPs (65%) and CuONPs (80%), CuO/ZnO nanocomposite showed superior photocatalytic activity for the breakdown of MB with a high percentage degradation efficiency (85%). The photocatalytic degradation rate constant was evaluated by using a pseudo-first order kinetic equation. Additionally, the agar disc diffusion method was used to examine the antibacterial activity against two bacterial strains (Escherichia coli and Staphylococcus aureus) and a sizable zone of inhibition against the bacterial strains was found.
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... 32 When toxicity was assessed against HeLa cells 24-hour results showed a minimal effect. [36][37][38] The Minimum inhibitory concentration of MgO was calculated as 10mg/mL against E. faecalis. It has been shown to disrupt biofilm growth even seen in SEM analysis. ...
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