Paecilomyces echinosporus sp. nov., a species isolated from soil in China

Abstract

During a survey of entomopathogenic fungi in China, a new species of Paecilomyces was isolated from a soil sample collected from Anhui province in China. It is differentiated from previously described species based on the morphology of its minutely echinulate conidia and conidiophores that possess penicillate phialides. Phylogenetic analyses with ITS region indicate that it is distantly related to Isaria and a close relative of P. carneus. The new species, Paecilomyces echinosporus, is presented with its Latin diagnosis, English description, and illustration. The type isolate and holotype are deposited in the Research Center for Entomogenous Fungi of Anhui Agricultural University (RCEF).

Full text

  
MYCOTAXON

Volume 114, pp. 25–32 October–December 2010

Paecilomyces echinosporus sp. nov.,
a species isolated from soil in China

chenmingjun2007@yahoo.cn zhouna0116@yahoo.com.cn
zzli@ahau.edu.cn bhuang@ahau.edu.cn
1 Anhui Provincial Key Laboratory of Microbial Control
Anhui Agricultural University, Hefei 230036, P. R. China
sung97330@korea.kr
2 Mushroom Research Division, Dept of Herbal Crop Research
National Institute of Horticultural and Herbal Science
Suwon 404-707, Republic of Korea
        
Paecilomyces 
           
       
Isaria
P. carneusPaecilomyces echinosporus, 

 


Introduction
Paecilomyces
Penicillium

  Paecilomyces        
Isaria  Spicaria        
P. variotiiPaecilomyces    
Paecilomyces
    P. Paecilomyces  P.  Isarioidea  



         
      Paecilomyces    
Paecilomyces 
SordariomycetesEurotiomycetesP.Isarioidea
   
Isaria    
  P  Isarioidea
PIsarioidea Isaria
         Paecilomyces  
   Paecilomyces        
        
        Taifanglania, 
   
      Paecilomyces       

         
    Paecilomyces    
Isaria Paecilomyces
echinosporus
Materials and methods
Sample collection and strain isolation
          
   
       
  
             
        


Strain identification


        





DNA extraction



Paecilomyces echinosporus... 
Paecilomyces 

   
 Cordyceps militaris   
 Isaria amoenerosea   
I. cateniannulata
  
 I. cicadae  
I. coleopterorum

 
 I. farinosa  
 I. fumosorosea  
I. javanica
  
 I. tenuipes  
 Mariannaea camptospora   
 Metarhizium anisopliae
 anisopliae
 
 M. cylindrosporum

 
 M. flavoviride 
 flavoviride
 
 Nomuraea rileyi   
Paecilomyces carneus 

 
P. cinnamomeus 

 
 P. echinosporus 

 
 P. gunnii   
 P. lilacinus   
 P. marquandii   
 P. niphetodes   
 P. penicillatus   
 P. viridis   
 Taifanglania curticatenata 

 
            
     


PCR amplification and determination of ITS sequencing









          
    

Sequence alignment and phylogenetic analysis
          


           
        


            


Results
Taxonomy
Paecilomyces echinosporus  

Coloniae in agaro Czapekii ad 30–37 mm diam post 14 dies 25°C, in medio modice
sulcatae, albae, pulverulentae, margine regulari; reversum luteolum; 35°C haud crescit.
Hyphae vegetativae hyalinae, septatae, ramosae, leves, 2.0–3.5 µm latae. Apparatus
conidialis elongatus vel compactus, seu phialides singulae seu capitula verticillos ramorum
et phialidum ferentia; stipites ex hyphis aeriis orientes, vulgo 45–95 × 2.5 μm. Phialides
ad quinae verticillatae, 9.5–15.5 × 2.0–3.0 μm, e basi cylindrica et collulo angusto minus
quam 0.5 µm lato composita. Conidia unicellularia, minute echinulata, subglobosa vel
elllipsoidea, 2.7–5.0 × 2.0–3.0 μm. Chlamydosporae absentes.



         
     
         



      




Paecilomyces echinosporus... 
Paecilomycesechinosporus

Molecular Characteristics of Paecilomyces echinosporus
         
  
   
        

P. carneus  
P. echinosporus
       P. echinosporus    
P. marquandiiP. lilacinus
 P. carneus     
P. echinosporus
 
P. carneus
    
P. echinosporus, 


   
       Mariannaea
camptospora

Paecilomyces echinosporus... 
Discussion
Paecilomyces
    P. carneusP. gunniiP.marquandii, 
P. lilacinus 
P. carneusP. gunnii
P. carneus
P. gunnii        
    P. marquandii  P. lilacinus    
        
  P. marquandii  P. lilacinus    
  P. echinosporus     

    Paecilomyces  P. echinosporus
 P. carneus           
   
  P. carneus     P. echinosporus 
P. carneus
P. echinosporus  
           
P. carneus

      

Paecilomyces,P. echinosporus.
Acknowledgements


    
         
           

Literature cited
    Paecilomyces    Byssochlamys
        

           Metarhizium   






Isaria

Paecilomyces

        Metarhizium
cylindrospora Nomuraea viridula
 Isaria

           Cordyceps gunnii 

Paecilomyces

             Paecilomyces   
Taifanglania
Paecilomyces
Isarioidea
Paecilomyces

   Paecilomyces        

           Beauveria
bassianaDeuteromycotina: Hyphomycetes


  
    


              
    

  
