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Electrophysiological responses of four fungivorous coleoptera to volatiles of Trametes versicolor: implications for host selection

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Kai Drilling
Kai Drilling
Kai Drilling
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Konrad Dettner at University of Bayreuth
Konrad Dettner
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Fungi of the genus Trametes are known as important wood decomposers and are colonized by various species of Coleoptera and other arthropods. The aim of the present study was to investigate the importance of volatile chemical compounds as key attraction factors in recognition and host selection by species of Erotylidae (Dacne bipustulata, Tritoma bipustulata) as well as Cisidae (Sulcacis affinis) and Tenebrionidae (Diaperis boleti). Volatiles from freshly collected Trametes versicolor were collected by headspace sampling technique and identified by combined gas chromatography–mass spectrometry (GC–MS). To evaluate the biological significance of the volatiles we performed behavioural tests and recorded antennal responses of the fungus-inhabiting species by gas chromatography with electroantennographic detection (GC-EAD). The scent of T. versicolor was found to be dominated by sesquiterpenes; in GC-EAD 6 of these compounds elicited reproducible antennal signals in the tested species. Highly significant attraction effects to the fungus, the obtained odour samples and previously described fungal C8-compounds were observed in behavioural tests. The possibility to detect these chemical compounds as a key cue for host selection implicate that beetles are able to discriminate between fungi of different age as well as different stages of colonization.
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2009 Drilling, Dettner - Electrophysiological responses of four fungivorous coleoptera .
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Article
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  • Feb 2024
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Article
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... These findings may be similar to what occurs in mushrooms, where it has been suggested that reduction of octan-3-one through the activity of an alcohol dehydrogenase produces octan-3-ol [38], so the yeast could be producing a compound at an early stage of growth and metabolizing it at another, demonstrating the importance of the growth phase in the production of VOCs. This alcoholic compound (octan-3-ol) was also identified in the fungus Trametes versicolor in a study to determine the electrophysiological responses of Coleoptera to VOCs [39], as well as in Tuber species, where this compound shows phytotoxic activity [40]. ...
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Article
Full-text available
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  • MOLECULES
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Article
Full-text available
  • Aug 2023
  • ENVIRON SCI POLLUT R
Microbial volatile compounds (MVCs) are produced during the metabolism of microorganisms, are widely distributed in nature, and have significant applications in various fields. To date, several MVCs have been identified. Microbial groups such as bacteria and fungi release many organic and inorganic volatile compounds. They are typically small odorous compounds with low molecular masses, low boiling points, and lipophilic moieties with high vapor pressures. The physicochemical properties of MVCs help them to diffuse more readily in nature and allow dispersal to a more profound distance than other microbial non-volatile metabolites. In natural environments, plants communicate with several microorganisms and respond differently to MVCs. Here, we review the following points: (1) MVCs produced by various microbes including bacteria, fungi, viruses, yeasts, and algae; (2) How MVCs are effective, simple, efficient, and can modulate plant growth and developmental processes; and (3) how MVCs improve photosynthesis and increase plant resistance to various abiotic stressors.
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Learning about microbial language: possible interactions mediated by microbial volatile organic compounds (VOCs) and relevance to understanding Malassezia spp. metabolism
Article
Full-text available
  • Apr 2021
  • METABOLOMICS
Background Microorganisms synthesize and release a large diversity of small molecules like volatile compounds, which allow them to relate and interact with their environment. Volatile organic compounds (VOCs) are carbon-based compounds with low molecular weight and generally, high vapor pressure; because of their nature, they spread easily in the environment. Little is known about the role of VOCs in the interaction processes, and less is known about VOCs produced by Malassezia , a genus of yeasts that belongs to the human skin mycobiota. These yeasts have been associated with several dermatological diseases and currently, they are considered as emerging opportunistic yeasts. Research about secondary metabolites of these yeasts is limited. The pathogenic role and the molecular mechanisms involved in the infection processes of this genus are yet to be clarified. VOCs produced by Malassezia yeasts could play an important function in their metabolism; in addition, they might be involved in either beneficial or pathogenic host-interaction processes. Since these yeasts present differences in their nutritional requirements, like lipids to grow, it is possible that these variations of growth requirements also define differences in the volatile organic compounds produced in Malassezia species. Aim of review We present a mini review about VOCs produced by microorganisms and Malassezia species, and hypothesize about their role in its metabolism, which would reveal clues about host-pathogen interaction. Key scientific concepts of review Since living organisms inhabit a similar environment, the interaction processes occur naturally; as a result, a signal and a response from participants of these processes become important in understanding several biological behaviors. The efforts to elucidate how living organisms interact has been studied from several perspectives. An important issue is that VOCs released by the microbiota plays a key role in the setup of relationships between living micro and macro organisms. The challenge is to determine what is the role of these VOCs produced by human microbiota in commensal/pathogenic scenarios, and how these allow understanding the species metabolism. Malassezia is part of the human mycobiota, and it is implicated in commensal and pathogenic processes. It is possible that their VOCs are involved in these behavioral changes, but the knowledge about this remains overlocked. For this reason, VOCs produced by microorganisms and Malassezia spp. and their role in several biological processes are the main topic in this review.
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Field attraction of Coleoptera to odours of the wood-decaying polypores Fomitopsis pinicola and Fomes fomentarius
Article
  • Jan 1995
The attraction of beetles to odours of the wood-decaying polypore fungi F. pinicola and F. fomentarius was studied at one spruce Picea dominated and one birch Betula dominated locality in a semi-natural forest in C Sweden. Beetles of the family Cisidae breeding in F. pinicola were strongly attracted to the odour of this fungus. In contast, the species of a similar cisid guild associated with F. fomentarius was not attracted to any of the fungal odours. Two species of the anobiid genus Dorcatoma associated with F. pinicola and F fomentarius respectively, were not significantly attracted to odour host but were caught in large numbers at living fruiting bodies. This aggregation was probably due to response to attractive pheromones. -from Authors
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Host preference of Cisidae (Coleoptera) on tree-inhabiting fungi in Northern Norway
Article
  • Jun 1998
Fruiting bodies of tree-inhabiting "macrofungi" (Polyporaceae, Hymenochaetaceae, Corticiaceae and Tremellaceae) were collected in 1993-1996 in Troms county, northern Norway for investigation of host specificity of Cisidae species. Three methods of estimating the amount of fruiting bodies were compared: 1) dry weight, 2) calculation of volume and 3) measure of volume. They were all highly correlated (R = 0.79-0.96), but only method 1) and 3) were applicable irrespective of shape and therefore used in the statistical analyses concerning host preferences. 31 different species and more than 1 000 units of tree-living fungi were investigated and yielded about 15 500 individuals and 13 species of Cisidae. The cisid species showed preference for one single genus or species of fungi. Cis boleti, Cis hispidus and Octotemnus glabriculus preferred Trametes spp., Cis comptus preferred Cerrena unicolor, Cis lineatocribratus and Cis jacquemarti preferred Fomes fomentarius, while Cis punctulatus preferred Trichaptum spp. Orthocis festivus and Ropalodontus strandi appeared to be even more specific, with occurrence exclusively in Stereum rugosum and Fomes fomentarius, respectively. Cis bidentatus showed no specific host selection (polyphagous). A spatial segregation of the cisids R. strandi, C. jacquemarti and C. lineatocribratus was evident regarding height above ground of the fungus F. fomentarius. Microclimatic factors cannot explain the host selection of the species, but Sulcacis affinis, Sulcacis fronticornis and Ennearthron cornutum were exclusively found in fungi in warm and dry positions.
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Pheromones affecting flying beetles colonizing the polypores Fomes fomentarius and Fomitopsis pinicola
Article
  • Sep 1997
The existence of long-range pheromones in five beetle species breeding in polypore fruiting bodies was studied in a field experiment. Species included were: Dorcatoma robusta Strand (Anobiidae), Cis jacquemarti Mellie (Cisidae), and Bolitophagus reticulatus (L.) (Tenebrionidae) breeding in Fomes fomentarius (L. ex Fr.), as well as Dorcatoma punctulata Mulsant & Rey and Cis glabratus Mellie breeding in Fomitopsis pinicola Fr. (Karst). Catches of these species in window traps baited with pieces of polypores, together with females or males of the same species, were compared with catches in traps baited with only polypores. Catches of D. punctulata and C. jacquemarti were low, and no B. reticulatus were caught. Males of D. robusta were strongly attracted by conspecific females. In this species, only the females were attracted to host odour. No pheromone attraction was demonstrated in C. glabratus, in which males and females were about equally attracted to the odour of their host.
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Occurrence of Edible Fungi and Other Macromycetes on Tree Stumps Over a Sixteen Year Period
Article
  • Jan 1993
Occurrence of fruit bodies of 30 macromycete species was followed between 1975 and 1991 on stumps of spruce, pine, birch and aspen near Helsinki, Finland. Armillaria mellea (coll.) and Kuehneromyces mutabilis persisted on some stumps for 13 yr, and Hypholoma capnoides was found on several stumps for 12 yr. Stereum sanguinolentum, Chondrostereum purpureum and Trametes spp. tended to disappear after 3-7 yr., Skeletocutis amorpha reached maximum frequency after five yr then declined; and Galerina marginata coll., Ischnoderma benzoinum, Pseudohydnum gelatinosum and Hygrophoropsis aurantiaca appeared on stumps after 6-7 yr. Frequency of Armillaria mellea fluctuated greatly from one year to the next. -from Author
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