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Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector

Authors:
Luis Herrera-Estrella at Center for Research and Advanced Studies of the National Polytechnic Institute
Luis Herrera-Estrella
G Van den Broeck
G Van den Broeck
G Van den Broeck
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R Maenhaut
R Maenhaut
R Maenhaut
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Marc Van Montagu at Ghent University
Marc Van Montagu
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Abstract

A chimaeric gene, consisting of the 5'-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimaeric gene is light-inducible in chloroplast-containing transformed tissue.
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© Nature Publishing Group1984
© Nature Publishing Group1984
© Nature Publishing Group1984
© Nature Publishing Group1984
© Nature Publishing Group1984
© Nature Publishing Group1984
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... these experiments either the entire gene was introduced (Brol et al., 1984) or the promoter was fused to an assayable g (Herrera-Estrella et al., 1984). The construction was introd ed either into callus which was artificially greened with cytoki (Broglie et al., 1984) or with a partially disarmed T-DNA wh confers, in addition to the gene of interest, a cytokinin synth but not an auxin synthesis capacity on the transformed p1 material (Herrera-Estrella et al., 1984). Such experiments h, only been reported on the expression of pea SSU genes in petu or tobacco. ...
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  • EMBO J
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... This study demonstrated that the light regulatory mechanism of gene expression is conserved among different plant species. To find out the location of the DNA sequences responsible for light inducible gene expression, a study was done by transferring a chimeric gene (product of two or more genes) to tobacco cells containing chloroplast (Herrera-Estrella et al., 1984). The study revealed that 5′ flanking sequences (side sequences) of the rbcS genes contain the DNA sequences responsible for light inducible gene expression. ...
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Full-text available
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Expression of the Gene Coding for the Small Subunit of Ribulosebisphosphate Carboxylase during Differentiation of Tobacco Plant Protoplasts
Article
  • Oct 1982
A hybridization probe was used to study the regulation of expression of the gene coding for the small subunit of ribulose 1,5-bisphosphate carboxylase, during functional differentiation of protoplasts. A library of cDNA from poly(A)-containing RNA extracted from specially treated tobacco leaves was constructed in the plasmid pBR322 by blunt-end ligation. This library was screened by colony hybridization with 32P-labelled cDNA prepared from mRNA coding for the precursor of the small subunit. A positive colony was identified containing recombinant plasmids with a nucleotide sequence homologous to this mRNA. These plasmids, bound to diazobenzyloxymethylated cellulose paper, were then used as a hybridization probe. The results showed unambiguously that the small subunit was not transcribed in protoplasts but was transcribed in undifferentiated white and chlorophyll-containing green callus cultures derived from protoplasts. The discrepancy between these results and those obtained with classical techniques is discussed.
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