Content uploaded by Zaid Ashiq Khan
Author content
All content in this area was uploaded by Zaid Ashiq Khan on Apr 09, 2019
Content may be subject to copyright.
Submit Manuscript | http://medcraveonline.com
Introduction
LAB (Lactic Acid Bacteria) has very big importance industrially
and is used for milk preservation, fermentation and cheese, yogurt,
and ker and butter milk. Species from the Gram Positive bacteria
group are used which includes the genera Lactobacillus, Leuconostoc,
Lactococcus, Streptococcus and Pediococcus. They can be recognized
from their fermentative ability and they are enriching nutrients,
improving organoleptic attributes, improving food safety and also
provide health benets.1‒4
Milk is highly perishable in characteristics and LAB is used to
prolong its shelf life with milk fermentation. It also helps to preserve
the nutritional components of milk. The fermentation of milk with
the usage of LAB is produced high quality milk products and they
have high organoleptic attributes. Recently a variety of products are
developed from fermented milk to facilitate health sector, like to
prevent toxins, which are produced by pathogens and some spoilage
bacteria.1,3,5,6
LAB presence in the milk fermentation is immunizing starter
cultures. While milk is also known as natural habitats of LAB.7,8 Milk
fermentation technology is generally cost effective and relatively
simple. LAB is used on the other hand as important starter cultures
in large scale under controlled conditions. While comparing with
the products under natural fermentation, LAB has potential starter
cultures which reduces and dominate the microorganism’s diversity
in fermented milk products. Using LAB in preservation and milk
fermentation improves the quality and milk palatability.
The main objective of this review paper is to facilitate the reader
with the summarized data. Most of aspects related to LAB functions
in preservation and milk fermentation are reviewed. The articles and
researches already done on health properties of LAB, preservative
and antimicrobial property of LAB by various researchers were also
combined and reviewed.
Milk fermentation (functions of Lactic Acid Bacteria)
LAB (Lactic Acid Bacteria) is predominating of micro ora in
milk products, in dairy products many species are involved.9 LAB
can be divided into two groups in dairy fermentation according
their growth optimum, Mesophilic LAB have 20 to 30°C growth
temperature and thermophilic have growth optimum from 30 to 45°C.
Western and Northern European countries products have Mesophilic
bacteria, while sub-tropical countries products have thermophilic
bacteria. During the fermentation of glucose, the LAB is divided
into two groups, Homofermentative and Heterofermentative LAB.
Homofermentative bacteria’s are Pediococcus, Lactococcus and
Streptococcus, lactic acid is sole product produced in the fermentation
of glucose. While, Heterofermentative bacteria’s are Leuconostoc and
Weissella produce CO2, lactate and ethanol from glucose.10‒12
Along with milk products LAB is used extensively in food
fermentation. LAB has proteolytic activity and is very important
to produce avor compound in end product.13 For the growth of
microorganisms, proteolytic system in LAB is very important. It
involves casein utilization in LAB cells; in fermented milk products
they produce organoleptic properties.13,14
LAB plays vital role in fermentation of milk and milk products.
Various LAB strains are used as starter cultures in milk industry as
shown in Table 1. These starter cultures were collected from sequence
activity of LAB and then they were isolated, selected and conrmed.
LAB has most important property to acidify milk and gives texture
and avor because of converting milk protein.15 Fermented milk has
characteristics to produce fresh and mild acid taste like yogurt and
cheese.16,17
Lactic Acid Bacteria with health stimulating property
Due to the health property of fermented milk and milk products
it became highly consumed with high demand (Table 2). Through
several mechanisms, fermentation of milk is contributing in human
health.3 Certain strains of Lactic Acid Bacteria (LAB) like genera
MOJ Food Process Technol. 2018;6(5):414‒416. 414
© 2018 Rakhmanova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License,
which permits unrestricted use, distribution, and build upon your work non-commercially.
A mini review fermentation and preservation: role
of Lactic Acid Bacteria
Volume 6 Issue 5 - 2018
Aizhan Rakhmanova,1 Zaid Ashiq Khan,2
Kamran Shah3
1College of Food Science, Northwest Agriculture and Forestry
University, China
2College of Agriculture Economics and Management, Northwest
Agriculture and Forestry University, China
3College of Horticulture, Northwest Agriculture and Forestry
University, China
Correspondence: Aizhan Rakhmanova, College of Food
Science, Northwest Agriculture and Forestry University, Yangling,
Shaanxi, China, Tel +8613299091332,
Email aizhan020994@gmail.com
Received: August 31, 2018 | Published: September 10, 2018
Abstract
The main focal point of this review is the investigation of the roles of LAB (Lactic
Acid Bacteria) in food preservation and milk fermentation. The LAB is composed
of Gram-Positive, cocci, rods, non-spore forming and is producing lactic acid at
the time of fermentation process of carbohydrates. LAB is used from ancient times
for fermentation of food and dairy items. But nowadays it is the main attention
phenomenon for scientific world. LAB has unique properties and has the ability
to produce Bacteriocins, which is anti-microbial compound. LAB is also used for
health medical treatment and is also used as substitute for chemicals in the process
of food preservation. In the recent years, the scientific reports show that LAB has
many positive health effects. LAB is used as starter culture in milk fermentation.
LAB also gives flavor to yogurt and produces acid in milk fermentation and works as
preservative agent. Due to dynamic characteristics of LAB it is important to educate
the world about LAB vital roles in food industry and its hygiene properties.
Keywords: milk, preservation, fermentation, lactic acid bacteria
MOJ Food Processing & Technology
Mini Review Open Access
A mini review fermentation and preservation: role of Lactic Acid Bacteria 415
Copyright:
©2018 Rakhmanova et al.
Citation: Rakhmanova A, Khan ZA, Shah K. A mini review fermentation and preservation: role of Lactic Acid Bacteria. MOJ Food Process Technol.
2018;6(5):414‒417. DOI: 10.15406/mojfpt.2018.06.00197
Lactobacillus are highly utilized for health promoting bacteria.18
Certain Lactobacillus strains are believed to Show immune modulator
activity, anti-hypertensive, calcium binding activity and anti-cancer.19
Table 1 The main Lactic Acid Bacteria associated with milk and milk product fermentation
Species/ Subspecies Their main uses in different milk product References
Lactococcus
Lc. Lactic subsp. lactis Mesophilic starter used for many cheese types, butter and butter milk. Broome et al. (2003) &
Wouters et al.8
Lc. lactis subsp. actis biovar
diacetylactis Used in Gouda, Edam, Sour cream and lactic butter and butter milk. Wood(1997), Leroy & De
Vusyt (2004)
Lc. lactis subsp. cremoris Mesophilic starter used for many cheese types, butter and butter milk. Weerkam et al. (1996)
Streptococcus
Sc. thermophilus Thermophilic starter used for yogurt and many cheese types’ particularly hard
and semi hard high-cook cheeses.
Broome et al. (2003) &
Beresford et al. (2001)
Lactobacillus
Lb. acidophilus Probiotic adjunct culture used in cheese and yogurt. Brigiler-Marco et al.(2007)
Lb. delbrueckii subsp. bulgaricus Thermophilic starter for yogurt and many cheese types, particularly hard and
semi-hard high-cook cheeses. Salaterry et al. (2010)
Lb. delbrueckii subsp. lactis Used in fermented milks and high-cook cheese. Broome et al. (2003) &
Giraffa26
Lb. helveticus Broome et al. (2003) &
Grifth & Tellez17
Lb. casei Thermophilic starter for fermented milks and many cheese types, particularly
hard and semi hard high-cook. Briggs (2003), Kongo16
Lb. plantarum Probiotic milk and cheese ripening adjunct culture. Cheese ripening adjunct
culture. Leroy & De Vuyst (2004)
Lb. rhamnosus Probiotic adjunct culture used in cheese. Coppola et al. (2005)
Leuconostoc
Ln. mesenteroides subsp. remoris Mesophilic culture used for Edam, Gouda, fresh cheese, Lactic butter and sour
cream.
Weerkam et al. (1996) &
Slattery et al. (2010)
Lb, Lactobacillus; Lc, Lactococcus; Ln, Leuconostoc; Sc, Streptococcus; subsp, Subspecies.
Table 2 Health benets when milk is fermented
Effect of fermentation Changes in milk Effect on health
Increase in Lactic Acid
Bacteria levels
Reduced lactose contents in milk Reduced content
of bad bacteria
No diarrhea and bloating
Improved gut health
Prevention of protection from bacterial vaginosis and
fungal infections in women
Ability to digest remaining lactose in the fermented
milk and use as energy source
Breakdown to shorter
chain proteins
Identication of casein peptides and whey peptides
with functional properties
Easier digestion
Some with anti-hypertensive effects
Some with pain relief effects
Some with immune enhancing properties
Some with calcium binding bone building properties
Increased acidity Sharpness of taste Prevents harmful bacterial growth in milk
Source: http://whqlibdoc.who.int/publications/2003/9241591196.pdf and ftp://ftp.fao.org/docrep/fao/007/y5686e/y5686e00.pdf
A mini review fermentation and preservation: role of Lactic Acid Bacteria 416
Copyright:
©2018 Rakhmanova et al.
Citation: Rakhmanova A, khan ZA, Shah K. A mini review fermentation and preservation: role of Lactic Acid Bacteria.. MOJ Food Process Technol.
2018;6(5):414‒417. DOI: 10.15406/mojfpt.2018.06.00197
Lactic Acid Bacteria producing anti-microbial
compounds
During fermentation process Lactic Acid Bacteria (LAB) produces
anti-microbial metabolites, include organic acids like propionic,
acetic acid and lactic acids as nal products. They create unfavorable
environment to spoilage and pathogenic microorganism’s growth.20 In
habitory compounds includes Bacteriocins and hydrogen peroxide are
against other bacteria.21 According to some studies LAB fermentation
is preventing diarrheal diseases with the characteristics of changing
composition of microorganisms in intestines.22 LAB also produces
Bacteriocins which are protein anti-microbial agents.23
Functional starter cultures (role of Lactic Acid
Bacteria)
Application of LAB (Lactic Acid Bacteria) as starter strains in the
manufacturing of different fermented milk products is very important.
Particularly, L. lactis, L. helveticus, streptococcus thermophiles, L.
delbruicki sub sp. The frequently used as milk starter cultures are
bulgaricus. Yoghurt is made with L. bulgaricus and S. thermophilus
and Lactococcus casei is found in cheeses. The manufacturer of
milk fermented products needs to select proper and balanced amount
of LAB used for starter cultures to make their desirable avor and
texture.24
Lactic acid bacteria as preservative
It is known that milk is perishable material. The normal milk can
be kept stored just for few hours, while the Lactic acid Bacteria (LAB)
fermented milk product can be stored for the whole year. Some variety
of cheese can be stored for 5 years. Preservation of milk products with
LAB fermentation is the cheapest technique. LAB has long history of
consumption and application in cheese processing.25,26 The fermented
food is more popular then unfermented according to consumers
because of its organoleptic properties. It reduces pH below 4°C
because of acid production and it stops pathogenic microorganism’s
growth. These microorganisms can produce disease and making milk
spoiled.27
Conclusion
LAB’s (Lactic Acid Bacteria) are gram positive organism used
as starter cultures. S. thermophilus, L. helveticus, L. lactis and L.
delbruckii subsp. bulgaricus are used mostly. In fermented foods
LAB shows numerous anti-microbial roles because of production of
organic acids. By producing Bacteriocins some strains are helpful
in the fermented milk preservation. Milk fermentation is protable
due to decontamination of toxins, preservation and improving milk
quality. Due to the good taste and texture it is also completing
consumer expectations. Fermentation with LAB is also effective and
cheap preservation method. It also improves nutritional value of milk
products. This technology must be developed further to facilitate the
ease and safety of application in poor rural resource setting.
Acknowledgements
None.
Conict of interest
Author declares that there is none of the conicts.
References
1. Panesar PS. Fermented Dairy Products: Starter Cultures and Potential
Nutritional Benets. Food Nutr Sci. 2011;2(1):47‒51.
2. Liu SN, Han Y, Zhou ZJ. Lactic Acid Bacteria in Traditional Fermented
Chinese Foods. Food Res Int. 2011;44(3):643‒651.
3. Sharma R, Sanodiya BS, Bagrodia D, et al. Efcacy and Potential of
Lactic Acid Bacteria Modulating Human Health. Int J Pharma Bio Sci.
2012;3(4):935‒948.
4. Steele J, Broadbent J, Kok J. Perspective on the Contribution of Lactic
Acid Bacteria to Cheese Flavour Development. Curr Opin Biotechnol.
2013;24(2):135‒141.
5. Shah NP. Functional Cultures and Health Benets. Int Dairy J.
2007;17(11):1262‒1277.
6. Ali A. Benecial role of Lactic Acid Bacteria in food Preservation and
Human Health: A review. Res J Microbiol. 2010;5(12):1213‒1221.
7. Delavenne E, Mounier J, Deniel F, et al. Biodiversity of Anti‒fungal Lactic
Acid Bacteria Isolated from Raw Milk Samples from Cow, Ewe and Goat
over One‒Year Period. Int J Food Microbiol. 2012;155(3):185‒190.
8. Wouters JTM, Ayad EHE, Hugenholtz J, et al. Microbes from Raw Milk
for Fermented Dairy Products. Int Dairy J. 2002;12(2‒3):91‒109.
9. Ayad EHE, Nashat S, El‒Sedek N, et al. Selection of wild Lactic Acid
Bacteria isolated from traditional Egyptian dairy products according to
production and technological criteria. Food Microbiol. 2004;21(6):715‒725.
10. Capice E, Fitzgerald GF. Food Fermentation: Role of Microorganisms
in Food Production and Preservation. Int J Food Microbiol.
1999;50(1‒2):131‒149.
11. Jay JM. Modern Food Microbiology. 6th ed. USA: An Aspen Pubication,
Aspen Publishers, Inc. Gaithersburg; 2000:113‒130.
12. Kuipers OP, Buist G, J Kok. Current Strategies for Improving Food
Bacteria. Res Microbiol. 2000;151(10):815‒822.
13. Moulay M, Benlancen K, Aggad H, et al. Diversity and Technological
Properties of Pre‒dominant Lactic Acid Bacteria Isolated from Algerian
Raw Goat Milk. Adv Environ Biol. 2013;7(6):999‒1007.
14. Yamina M, Wassila C, Kenza Z, et al. Physico Chemical and
Microbiological Analysis of Algerian Raw Camel’s Milk and Identication
of predominating thermophilic Lactic Acid Bacteria. J Food Sci Eng.
2013;3:55‒63.
15. Mayra‒Makinen A, Bigret M. Industrial Use and Production of Lactic Acid
Bacteria. In: Salminen S, Von Wright A, Ouwehands A, editors. Lactic Acid
Bacteria. Microbiological and Functional Aspects. New York: Marcel
Dekker, Inc; 2004:175‒198.
16. Kongo JM. Lactic Acid Bacteria as Starter‒Cultures for Cheese
Processing: Past, Present and Future Developments. 2013.
17. Grifth MW, Tellez AM. Lactobacillus helveticus: The Proteolytic System.
Front Microbiol. 2013;4:30.
18. Saxelin M, Tynkkynen S, Mattila‒Sandholm T, et al. Probiotic and Other
Functional Microbes: from Markets to Mechanisms. Curr Opin Biotechnol.
2005;16(2):204‒211.
19. Nouaille S, Ribeiro LA, Miyoshi A, et al. Heterologous Protein
Production and Delivery Systems for Lactococcus Lactis. Genet Mol Res.
2003;2(1):102‒111.
20. Rattanacaikunsopon P, Phumkhachorn P. Lactic Acid Bacteria: Their
Anti‒Microbial Compounds their Uses in Food Production. Ann Biol Res.
2010;1(4):218‒228.
A mini review fermentation and preservation: role of Lactic Acid Bacteria 417
Copyright:
©2018 Rakhmanova et al.
Citation: Rakhmanova A, Khan ZA, Shah K. A mini review fermentation and preservation: role of Lactic Acid Bacteria. MOJ Food Process Technol.
2018;6(5):414‒417. DOI: 10.15406/mojfpt.2018.06.00197
21. Oyewole OB. Lactic Fermented Foods in Africa and their Benets. Food
Control. 1997;8(5‒6):289‒297.
22. Schnurer J, Magnusson J. Anti‒Fungal Lactic Acid Bacteria as Bio
Preservatives. Trends Food Sci Technol. 2005;16(1‒3):70‒78.
23. Carolissen‒Mackay V, Arendse G, Hasting JW. Purication of Bacteriocins
of Lactic Acid Bacteria: Problems and Pointers. Int J Food Microbiol.
1997;34(1):1‒16.
24. Derek AA, Joost VDB, Inge MKM, et al. Anaerobic Homolactate
Fermentation with Saccharomyces Cerevisiae Results in Depletion of ATP
and Impaired Metabolic Activity. FEMS Yeast Res. 2009;9(3):349‒357.
25. Aquilanti L, Dell’Aquila L, Zannini E, et al. Resident Lactic Acid
Bacteria in Raw Milk Canestrato Pugaliese Cheese. Lett Appl Microbiol.
2006;43(2):161‒167.
26. Giraffa G, Chanishvili N, Widyastuti Y. Importance of Lactobacilli in Food
and Feed Biotechnology. Res Microbiol. 2010;161(6):480‒487.
27. Ananou S, Maqueda M, Martinez‒Bueno M, et al. Biopreservation, An
Ecological Approach to Improve the Safety and Shelf‒Life of Foods.
FORMATEX. 2007:1‒12.