ArticlePDF Available

Recent advances in biological pretreatment of microalgae and lignocellulosic biomass for biofuel production

Authors:
UNCORRECTED PROOF
/>G>P:;E>:G=0NLM:BG:;E>"G>K@R/>OB>PL QQQ  QQQQQQ
HGM>GMLEBLML:O:BE:;E>:M0<B>G<>!BK><M
/>G>P:;E>:G=0NLM:BG:;E>"G>K@R/>OB>PL
CHNKG:EAHF>I:@>PPP>EL>OB>K<HF
/><>GM :=O:G<>L BG ;BHEH@B<:E IK>MK>:MF>GM H? FB<KH:E@:> :G= EB@GH<>EENEHLB< ;BHF:LL
?HK ;BH?N>E IKH=N<MBHG
%HLL:BG * 7:;>=p: 0N>ER DM>Kp: 'NGAN: 6NGp: $NHR:G 7A:G@p: #:BL:E + P:=p: 5B:G@ANB .Bp:p p '+ 0:ANp;
:!).552 5, 55* '4* /525-/)'2 4-/4++8/4- /'4-9; #4/<+89/:?  %;+,; 5'* &.+40/'4-  /'4-9; ./4'
;49:/:;:+ 5, .+3/)'2 "+).4525-? ');2:? 5, .+3/9:8? #4/<+89/:? 5, !:;::-'8:  !:;::-'8: +83'4?
/1& )" &+#,
+?=58*9
)B@GH<>EENEHLB< ;BHF:LL
*B<KH:E@:> IK>MK>:MF>GM
BHEH@B<:E IK>MK>:MF>GM
:<M>KB:E IK>MK>:MF>GM
#NG@:E IK>MK>:MF>GM
)B@GBGHERMB< >GSRF>
4ABM> KHM ?NG@B
01/ 1
BHEH@B<:E IK>MK>:MF>GM - BL : IKHFBLBG@ :IIKH:<A ?HK MK>:MBG@ FB<KH:E@:> :G= EB@GH<>EENEHLB< ;BHF:LL ) 
=NKBG@ ;BH?N>EL IKH=N<MBHG MA:M NL>L FHLMER ?NG@:E :G= ;:<M>KB:E LMK:BGL HK MA>BK >GSRF>L -K>MK>:MF>GM PBMA
?NG@B K>JNBK>L EHG@ BG<N;:MBHG MBF> P>>DL MH FHGMAL PA>K>:L ;:<M>KB:E :G= >GSRF:MB< IK>MK>:MF>GML <:G ;>
<HFIE>M>= ;R HGER : ?>P AHNKL MH =:RL +>O>KMA>E>LL ?NG@:E IK>MK>:MF>GM >LI><B:EER PBMA PABM>KHM ?NG@B 4/#
BL IK>=HFBG:GMER NL>= BG - H? ;BHF:LL ?HK BML AB@A >?i<B>G<R :G= =HPGLMK>:F RB>E=L <<HK=BG@ MH MA> K><>GM
K>IHKML =>EB@GBi<:MBHG H? )  ;R 4/# F:R O:KR ;>MP>>G  :G=  PBMA : F:QBFNF  BG<K>:L> BG MA>
;BH?N>E RB>E= HFI:K>= MH MA> NGMK>:M>= FB<KH:E@:> ;BHF:LL MA> =HPGLMK>:F RB>E=L H? MA> K>LI><MBO> ;BH?N>EL
P>K> ?HNG= MH ;> BG<K>:L>= ;R  :?M>K ;:<M>KB:E IK>MK>:MF>GM PABE> >GSRF:MB< IK>MK>:MF>GM BFIKHO>= :L
FN<A :L  H? MA> iG:E RB>E= !>LIBM> MA> K>LNEML :K> IKHFBLBG@ >QIEHBM:MBHG H? - HG E:K@> L<:E> BL LMBEE ;HMME>
G><D>= ;R LHF> M><AGH><HGHFB< ANK=E>L PAB<A G>>= MH ;> HO>K<HF> MAKHN@A ?NKMA>K ?NG=:F>GM:E :G= :IIEB>=
K>L>:K<A>L 1ABL I:I>K IK>L>GML : <HFIK>A>GLBO> :G= BG=>IMA K>OB>P HG - ?HK )  :G= FB<KH:E@:> ;BHF:LL ;R
?H<NLBG@ HG MA> K>E>O:GM HO>KOB>PL :G= I>KLI><MBO>L M><AGHEH@B<:E :IIKH:<A>L F><A:GBLFL BGjN>G<BG@ ?:<MHKL
:G= K><>GM K>L>:K<A IKH@K>LL>L #BG:EER <A:EE>G@>L :G= ?NMNK> HNMEHHDL :K> =BL<NLL>= BG MA> <HG<EN=BG@ L><MBHGL
1. Introduction
BH<HGO>KLBHG H? FB<KH:E@:> :G= EB@GH<>EENEHLB< ;BHF:LL )  BGMH
;BH?N>EL A:L K><>BO>= FN<A :MM>GMBHG HO>K MA> I:LM R>:KL MH FBMB@:M>
MA> @KHPBG@ =>F:G= ?HK K>IE:<BG@ LB@GB?B<:GM IHKMBHGL H? ?HLLBE ?N>EL
;R :EM>KG:MBO> >G>K@R -K>MK>:MF>GM BL :G >LL>GMB:E LM>I BG MA> ;BH
<A>FB<:E KHNM> H? ;BHF:LL <HGO>KLBHG =N> MH MA> <HFIE>Q LMKN<MNK>
:G= I>KLBLM>GM G:MNK> H? MA> <>EE P:EE <HFIHG>GML BG MA> ;BHF:LL E
MAHN@A MA>KFH<A>FB<:E F>MAH=L :K> BG MA> F:BGLMK>:F H? ;BHF:LL
IK>MK>:MF>GM MA>K> :K> LHF> M><AGB<:E >GOBKHGF>GM:E :G= ><HGHFB<
BLLN>L PBMA MA>L> :IIKH:<A>L 1A>K>?HK> BM BL G><>LL:KR MH iG= HNM
: FHK> LNLM:BG:;E> IK>MK>:MF>GM M><AGHEH@R ?HK @>G>K:MBG@ ;BH?N>EL
EBD> ;BH:E<HAHEL ;BHAR=KH@>G :G= ;BH@:L &G MABL :LI><M ;BHEH@B<:E
IK>MK>:MF>GM - <HNE= ;> :G :MMK:<MBO> :EM>KG:MBO> MH MA> <HGO>G
MBHG:E IK>MK>:MF>GM F>MAH=L :L BM NL>L G:MNK:E FB<KHHK@:GBLFL HK MA>BK
>GSRF>L NG=>K MA> FBE= <HG=BMBHGL 89
1A> F:CHK :=O:GM:@>L H? - :K> EHP >G>K@R <HGLNFIMBHG LBFIE>
HI>K:MBG@ <HG=BMBHGL :G= >JNBIF>GM GH HK FBGBFNF BGAB;BMHK ?HKF:
MBHG EHP =HPGLMK>:F IKH<>LLBG@ <HLML :G= GH K>JNBK>F>GM ?HK K><R
<EBG@ MA> <A>FB<:EL :?M>K IK>MK>:MF>GM 89 L : K>LNEM - H??>KL :
@K>>G L:?> :G= <A>:I :IIKH:<A ?HK ;BHF:LL IK>MK>:MF>GM &G :==B
MBHG - <:G IKHOB=> HIIHKMNGBMR MH IKH=N<> O:KBHNL O:EN>:==>= IKH=
N<ML ?KHF MA> ;RIKH=N<ML HK F:BGLMK>:F <HFIHNG=L H;M:BG>= :?M>K
IK>MK>:MF>GM PAB<A BL IKHFBLBG@ ?HK :<AB>OBG@ ><HGHFB< :G= M><A
GB<:E OB:;BEBMR H? - &G I:KMB<NE:K - H? )  K>LNEML BG MA> ;K>:D
=HPG H? EB@GBG BGMH O:KBHNL LF:EE>K <HFIHNG=L MA:M <:G ;> NL>= :L
MA> LM:KMBG@ F:M>KB:EL ?HK @>G>K:MBG@ IA>GHEB< :<B=L O:GBEEBG O:GBEEB<
:<B= <BGG:FB< :<B= ;>GSHB< :<B= :G= LRKBG@:E=>AR=> 89 )BD>PBL>
- H? FB<KH:E@:> IKHOB=>L EBIB=L IKHM>BGL :G= <:K;HAR=K:M>L =>KBO>=
<HFIHNG=L PAB<A <:G ;> NL>= ?HK IKH=N<BG@ ?HH= LNIIE>F>GML iG>
((8+</':/549 $ iKLM @>G>K:MBHG $ L><HG= @>G>K:MBHG $ MABK= @>G>K:MBHG $ ?HNKMA @>G>K:MBHG ! :KRE:E<HAHE =>AR=KH@>G:L>L 10 b:SBGH;BL>MARE;>GSMAB:SH
EBG> LNE?HG:M> *- ;BH<A>FB<:E F>MA:GHE IHM>GMB:E - ;BHEH@B<:E IK>MK>:MF>GM /# ;KHPGKHM ?NG@B ,! <A>FB<:E HQR@>G =>F:G= !R- =R>=><HEHKBSBG@ I>KHQB=:L> $%$
@K>>GAHNL> @:L $),5 @ERHQ:E HQB=:L> %1  AR=KHQR;>GSHMKBSHE> %-& +AR=KHQRIAMA:EBFB=> )  EB?> <R<E> :LL>LLF>GM )  EB@GH<>EENEHLB< ;BHF:LL )B- EB@GBG I>KHQB=:L> )*0
E:<<:L> F>=B:MHK LRLM>F *G- F:G@:G>L> I>KHQB=:L> +% +AR=KHQR:<>M:GBEB=> +-  GHGIA>GHEB< <HFIHNG= -  IA>GHEB< <HFIHNG= --, IHERIA>GHE HQB=:L> ./ JNBGHG> K>=N<
M:L>L 0/# LH?MKHM ?NG@B 3- O>KL:MBE> I>KHQB=:L> 4/# PABM>KHM ?NG@B
HKK>LIHG=BG@ :NMAHK
3'/2 '**8+99 JQANCL>=N<G 5 .B
AMMIL=HBHK@CKL>K
/><>BO>= 'NG>/><>BO>=BGK>OBL>=?HKF ':GN:KR<<>IM>= ':GN:KR
O:BE:;E>HGEBG> QQQ
 a 
Renew Sust Energ Rev, 2019, 105: 105-128. doi: 10.1016/j.rser.2019.01.048.
UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
<A>FB<:EL ?HK IA:KF:<>NMB<:EL :G= EBO>LMH<D ?>>= 89 1ABL BGM>@K:M>=
- :G= ;BHK>iG>KR <HG<>IM <:G IKHOB=> : ;>MM>K LNLM:BG:;BEBMR MAKHN@A
@>G>K:MBG@ ;BH?N>EL BG : <HLM>??><MBO> :G= ><H?KB>G=ER F:GG>K 89 !>
LIBM> A:OBG@ MA> IHM>GMB:E BFIE>F>GM:MBHG H? - HG E:K@> L<:E> BL LMBEE
;HMME>G><D>= ;R LHF> D>R BLLN>L EBD> K>JNBK>F>GM H? EHG@ IK>MK>:MF>GM
MBF> EHLL H? <:K;HAR=K:M>L :G= EHP =HPGLMK>:F RB>E=L 89
1A>K> A:O> ;>>G GNF>KHNL LMN=B>L =HG> HG MA> IK>MK>:MF>GM H? ) 
:G= FB<KH:E@:> NLBG@ - MA:M K>IHKM>= IKHFBLBG@ HNM<HF>L <HFI:K>=
MH MA> K>LNEML H;M:BG>= ?KHF MA> NGMK>:M>= ;BHF:LL 89 4ABE> MA>
- BL ?HNG= MH ;> FHK> >QM>GLBO> BG )  MA:G BG FB<KH:E@:> :IIEB<:
MBHG H? MABL M><AGHEH@R BG MA> IK>MK>:MF>GM H? FB<KH:E@:> PHNE= ;> FHK>
IKHFBLBG@ ><:NL> FB<KH:E@:E LMKN<MNK> :G= <HFIHLBMBHG :K> FHK> ?:
OHK:;E> ?HK - )  <HGM:BG LB@GB?B<:GM :FHNGML H? EB@GBG BG MA>BK <>EE
P:EEL :G= ?HKF EB@GH<>EENEHLB< F:MKBQ BG MA> <>EE P:EEL PABE> FB<KH:E@:>
<HGM:BG LM:K<A EBIB= :G= IKHM>BG PBMAHNM :GR EB@GBG 89 )  BL NLN:EER
IK>MK>:M>= ?HK EB@GBG K>FHO:E :G= BFIKHOBG@ MA> =B@>LMB;BEBMR H? <>EEN
EHL> 89 ,G MA> HMA>K A:G= =BLKNIMBHG H? MA> <>EE P:EE :G= AR=KHERLBL
H? MA> F:<KHFHE><NE>L BL MA> IKBF:KR H;C><MBO> ?HK FB<KH:E@:> IK>MK>:M
F>GM 89 #NKMA>KFHK> MA> H;C><MBO> H? IK>MK>:MF>GM F:R :ELH O:KR
=>I>G=BG@ HG MA> ;BH<HGO>KLBHG IKH<>LL>L :L P>EE :L MA> MRI>L H? ;BH?N
>EL MH ;> IKH=N<>= #HK >Q:FIE> IKH=N<MBHG H? ;BH@:L MAKHN@A :G:>KH
;B< =B@>LMBHG :BFL MH AR=KHERS> MA> F:<KHFHE><NE>L PBMAHNM >QI><MBG@
: L>I:K:M> =>EB@GBi<:MBHG IKH<>LL ?HK )  89
1H :<AB>O> MA> M><AGH><HGHFB< OB:;BEBMR H? - ?HK BML E:K@>L<:E> >Q
IEHBM:MBHG LN;LM:GMB:E K>:EPHKE= K>L>:K<A >??HKML :K> LMBEE G><>LL:KR &G
MABL K>@:K= : <HFIK>A>GLBO> K>OB>P HG - PHNE= ;> O>KR A>EI?NE ?HK
MA> K>L>:K<A>KL MH <HF> :<KHLL MA> <NKK>GM =>O>EHIF>GML BG - >QBLMBG@
<A:EE>G@>L :G= IKHLI><MBO> K>L>:K<A G>>=L 1ABL I:I>K BL MA>K>?HK> =>
LB@G>= MH IK>L>GM :G BG=>IMA :G= <KBMB<:E K>OB>P HG MA> <NKK>GM LM:MNL
:G= =>O>EHIF>GML BG - ?HK LNLM:BG:;E> ;BH?N>EL IKH=N<MBHG ?KHF ) 
:G= FB<KH:E@:> 1A> <HGM>GM H? MA> I:I>K BL :KK:G@>= :L  HO>KOB>P
:G= ;:<D@KHNG= HG ;BH?N>EL ;BHF:LL <HFIHLBMBHG <HGO>KLBHG KHNM>L
:G= IK>MK>:MF>GM F>MAH=L  :IIKH:<A>L H? -  F><A:GBLFL H? -
 ?:<MHKL :??><MBG@ MA> >?i<B>G<R H? -  K>L>:K<A IKH@K>LL>L :G= =>
O>EHIF>GML :G=  <A:EE>G@>L EBFBM:MBHGL :G= ?NMNK> HNMEHHDL
2. Overviews and perspectives
 /5,;+29 :?6+9 ,++*9:5)19 '4* 9;9:'/4'(/2/:? /99;+9
BH?N>EL IKH=N<>= ?KHF )  :G= FB<KH:E@:> A:O> ;>>G <HGLB=>K>=
:L MA> FHLM IKHFBLBG@ :EM>KG:MBO>L MH ?HLLBE ?N>EL LBG<> MA>L> K>G>P:;E>
?N>EL A:O> FN<A IHM>GMB:E HO>K ?HLLBE ?N>EL BG MA> :LI><ML H? >G>K@R L><N
KBMR ><HGHFB< BLLN>L :G= >GOBKHGF>GM:E LNLM:BG:;BEBMR #B@  89
%HP>O>K ;BH?N>EL F:R :ELH K:BL> LHF> <HG<>KGL BG M>KFL H? IHEB<R M><A
GHEH@R :G= ?>>=LMH<DL #B@  89 4BMA MA> M><AGHEH@B<:E :=O:G<>
F>GML :G= IHEB<R =>O>EHIF>GML :<KHLL MA> PHKE= MA> =K:P;:<DL BGA>K
>GM BG ;BH?N>EL :K> >QI><M>= MH ;> FBGBFBS>= BG E:K@> >QM>GM &G MABL P:R
;BH?N>EL <HNE= ;> >LM:;EBLA>= :L MA> D>R :EM>KG:MBO> ?N>EL BG G>:K ?NMNK>
MA:M <:G MA>G K>IE:<> : F:CHK IHKMBHG H? ?HLLBE ?N>EL 89 1A> F:BG
LMK>:F ;BH?N>EL :K> ;BH:E<HAHEL ;BH>MA:GHE :G= ;BH;NM:GHE ;BH=B>L>E
;BH@:L ;BHHBE ;BHAR=KH@>G :G= ;BH@:LHEBG> 1A> ;BH?N>EL :K> ;KH:=ER
<E:LLBi>= BGMH ?HNK @>G>K:MBHGL $ MAKHN@A $ ;:L>= HG MA> G:MNK> H?
;BHF:LL :G= <HGO>KLBHG M><AGHEH@B>L NL>= BG MA> IKH=N<MBHG H? ;BH?N>E
#B@  89
1A> $ ;BH?N>EL :K> IKH=N<>= ?KHF LN@:KL LM:K<A O>@>M:;E> HBEL
:G= :GBF:E ?:ML 0N@:K;:L>= K:P F:M>KB:EL IKH=N<> MA> <A>:I>LM ;BH
?N>EL ?HK <HGM:BGBG@ : E:K@> :FHNGM H? ?K>> LN@:KL PAB<A FBGBFBS>L
GHM HGER MA> HI>K:MBHG LM>IL ;NM :ELH BM K>=N<>L MA> G>M <:IBM:E BG
O>LMF>GML %HP>O>K LN@:K <KHIL K>JNBK> L>E><MBO> <EBF:MB< <HG=BMBHGL
?HK @KHPMA PAB<A EBFBML MA>BK <NEMBO:MBHG :G= :O:BE:;BEBMR BG LHF> @>H
Fig. 1. -KHL :G= <HGL H? ;BH?N>EL
Fig. 2. E:LLBi<:MBHG H? ;BH?N>EL PBMA K>LI><MBO> K:P F:M>KB:EL
@K:IAB<:E K>@BHGL 89 L : K>LNEM <NKK>GMER LM:K<AR <KHIL :K> PB=>ER
NL>= ?HK @>G>K:MBG@ $ ;BH?N>EL 1A>L> <KHIL <:G ;> @KHPG :<KHLL MA>
PHKE= :G= H??>K K>E:MBO>ER EHG@>K LMHK:@> <:I:;BEBMR MA:G MA> LN@:K <KHIL
&G :==BMBHG <HGO>KLBHG H? LM:K<AR F:M>KB:EL BGMH ;BH?N>EL BL >:L>K :G=
IKH=N<>L : AB@A>K ;BH?N>E RB>E= <HFI:K>= MH $ :G= $ ?>>=LMH<DL
89 1A> F:CHK LM:K<AR ?>>=LMH<DL <HNE= ;> <>K>:EL  LM:K<A
MN;>KL :G= KHHML  LM:K<A E>@NF>L  LM:K<A :G= @K>>G
:G= BFF:MNK> ?KNBML L FN<A :L  LM:K<A 89 BH?N>EL IKH
=N<>= ?KHF )  :K> K>?>KK>= MH :L $ ;BH?N>EL PABE> FB<KH:E@:> IKH
=N<> $ ;BH?N>EL *B<KH:E@:> <:G ;> <NEMBO:M>= BG HI>G IHG=L HK IAHMH
;BHK>:<MHKL PBMAHNM K>JNBKBG@ :K:;E> E:G=L
EMAHN@A FHLM H? MA> <NKK>GM <HFF>K<B:E ;BH?N>EL :K> IKH=N<>= ?KHF
$ ?>>=LMH<DL MA>L> F:M>KB:EL :K> BG:=>JN:M> ?HK F>>MBG@ MA> K:IB=ER
@KHPBG@ @EH;:E =>F:G=L ?HK ;BH?N>EL 1A>K>?HK> @K>:M>K ?H<NL>L :K> GHP
@BO>G HG $ :G= $ ;BH?N>EL %HP>O>K <HGO>KLBHG H? )  :G= FB<KH:E
@:> ;BHF:LL BGMH ;BH?N>EL BL FHK> <HFIEB<:M>= MA:G PA:M BL K>JNBK>= ?HK
LN@:KL :G= LM:K<AR F:M>KB:EL L PBEE ;> =>L<KB;>= E:M>K 0><MBHG 
@>G>K:MBHG H? $ :G= $ ;BH?N>EL G><>LLBM:M> LHF> >LL>GMB:E LM>IL BG
<EN=BG@ IK>MK>:MF>GM H? ;BHF:LL ;>?HK> LN;C><MBG@ MA>F BGMH AR=KHERLBL
:G= ?>KF>GM:MBHG &G MA> <NKK>GM IK:<MB<> IK>MK>:MF>GM BL FHLMER =HG>
;R NLBG@ >G>K@RBGM>GLBO> MA>KFH<A>FB<:E :IIKH:<A>L PAB<A >O>GMN
:EER <HFIKHFBL> PBMA MA> LNLM:BG:;BEBMR H? $ :G= $ ;BH?N>EL
UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
1A> F:CHK LNLM:BG:;BEBMR BLLN>L BG ;BH?N>EL FHLMER K:BL> ?KHF MA> ><H
GHFB< :G= >GOBKHGF>GM:E :LI><ML PAB<A :K> I:KMB<NE:KER K>E:M>= MH MA>
?>>=LMH<DL IK>MK>:MF>GM ><HGHFR >GSRF> <HLML ?>KF>GM:MBHG >?i<:<R
:G= =HPGLMK>:F ;BH?N>EL K><HO>KR <HLML 89 1A> ;BH?N>E @>G>K:MBG@
IE:GML GHM HGER IKH=N<> >G>K@R ;NM :ELH <HGLNF> BM &G :==BMBHG MA>
IE:GML :ELH @>G>K:M> P:LM>L MA:M <HNE= IHM>GMB:EER ;> A:KF?NE ?HK MA>
>GOBKHGF>GM :G= ANF:G A>:EMA EMAHN@A ;BH?N>E BG=NLMKB>L BG @>G>K:E
M:D> LHF> LM>IL MH :==K>LL >G>K@R <HGLNFIMBHG :G= P:LM> IKH=N<MBHG
>GOBKHGF>GM:E LNLM:BG:;BEBMR LMBEE K>F:BGL : F:CHK <HG<>KG 4BMA MA> =>
O>EHIF>GM H? :=O:G<>= M><AGHEH@B>L ?HK :LL>LLBG@ >GOBKHGF>GM:E <HG
<>KGL EB?> <R<E> :LL>LLF>GM )  BL ?HNG= MH ;> HG> H? MA> FHLM >??><
MBO> :IIKH:<A &M >O:EN:M>L >GOBKHGF>GM:E BLLN>L H? MA> PAHE> IKH<>LL HK
>O>G :GR LM>I H? ;BH?N>E IKH=N<MBHG 89 *HK>HO>K )  <:G IKHOB=> :
<HFIK>A>GLBO> L<>G:KBH H? :GR ;BH?N>E HK BML IKH=N<MBHG IKH<>LL MAKHN@A
JN:GMBM:MBO> :G:ERLBL H? G>M >G>K@R :G= F:M>KB:E <HGLNFIMBHG <HNIE>=
PBMA JN:GMB?RBG@ MA> P:LM>L =BL<A:K@> :G= >FBLLBHG H? I:KMB<NE:M> F:M
M>KL LN<A :L @K>>GAHNL> @:L>L $%$ 89 #HK >Q:FIE> )  LMN=B>L ?HK
$%$ >FBLLBHGL ;R =B??>K>GM ;BH?N>EL A:O> K>O>:E>= MA:M :E@:E ;BH=B>L>E
>FBML LB@GB?B<:GMER EHP>K :FHNGML H? @K>>GAHNL> @:L>L MA:G MA> $%$
>FBMM>= ?KHF MA> <HKG >MA:GHE 89 1A> K>=N<>= $%$ >FBLLBHGL ?KHF
FB<KH:E@:E ;BH?N>EL <HNE= ;> :MMKB;NM>= MH MA> K><R<EBG@ H? ,p :L MA>
<:K;HG LHNK<> ?HK FB<KH:E@:> <NEMBO:MBHG 89
 53659/:/54 '4* 8+)'2)/:8'4)+ 5, (/53'99
1A> F:CHK <HFIHG>GML BG MA> <>EE P:EE H? )  :K> <>EENEHL>
 A>FB<>EENEHL>  :G= EB@GBG  89 1A>L>
<HFIHG>GML :K> EBGD>= MH@>MA>K :G= ?HKF : <HFIE>Q G>MPHKD DGHPG
:L EB@GH<>EENEHLB< F:MKBQ #B@  PAB<A IKHOB=> MA> K><:E<BMK:G<> BG MA>
;BHF:LL >EENEHL> BL : EBG>:K IHERF>K H? @EN<HL> ?HKF>= ;R CHBGBG@ MA>
@EN<HL> FHE><NE>L MH@>MA>K ;R β→ @ER<HLB=B< EBGD:@>L "QM>GLBO> AR
=KH@>G ;HG=BG@ :FHG@ MA> AR=KHQRE @KHNIL BG MA> <>EENEHL> FHE><NE>L
IKH=N<>L : <KRLM:EEBG> LMKN<MNK> MA:M BL <:EE>= FB<KHi;KBE *B<KHi;KBEL
F:D> <>EENEHL> FHE><NE>L FHK> LMK>G@MA :G= KB@B= PAB<A BG MNKG BG
<K>:L>L MA> <KRLM:EEBGBMR H? <>EENEHL> %>FB<>EENEHL> BL : A>M>KHIHERF>K
:G= <HGLBLML H? LAHKM EBG>:K :G= AB@AER ;K:G<A>= <A:BGL H? =B??>K>GM A>Q
HL> :G= I>GMHL> FHGHF>KL PBMA LHF> LN@:K :<B=L 89
)B@GBG BL : A>M>KHIHERF>K <HFIHL>= H? AR=KHQRIA>GREIKHI:GHB=
NGBML <:EE>= FHGHEB@GHEL HK EB@GBG IK><NKLHKL 1A> FHGHEB@GHEL :K>
EBGD>= MH >:<A HMA>K </' O:KBHNL >MA>K  ,  :G= <:K;HG
Fig. 3. 0MKN<MNK> H? : MRIB<:E ;BHF:LL : =C:<>GM <>EEL ; >EE P:EE E:R>KL < !BLMKB;N
MBHG H? EB@GH<>EENEHLB< <HFIHG>GML BG L><HG=:KR P:EE :G= = #HKF:MBHG H? EB@GH<>EENEHLB<
F:MKBQ />=K:PG PBMA FH=Bi<:MBHG ?KHF 89
<:K;HG    ;HG=L PBMA =B??>K>GM =>@K>> H? F>MAHQRE:MBHG 89 1A>K>
:K> MAK>> MRI>L H? FHGHEB@GHEL G:F>ER 6<HNF:KHRE <HGBiG>KRE :E<HAHE
:G= LBG:IRE :E<HAHE MA:M :K> <HGLBLM>= H? MA> IK><NKLHK 6AR=KHIA>GRE
% @N:B:<RE $ :G= LRKBG@RE 0 K>LI><MBO>ER 89 1A> ?HKF:MBHG H?
EB@GBG FHE><NE> BL =HG> ;R MA> K:=B<:E IHERF>KBS:MBHG H? MA> ;:LB< LMKN<
MNK:E NGBML PA>K> =B??>K>GM MRI>L H? EBGD:@>L :K> ?HNG= BG O:KRBG@ JN:G
MBMB>L =>I>G=BG@ HG MA> MRI>L H? ;BHF:LL 89 1A> , :G= ;HG=L
IKHOB=> K><:E<BMK:G<> BG EB@GBG MH AR=KHERMB< :MM:<D PABE> >F;>=F>GM
H? <>EENEHL> iE:F>GML BGMH MA> EB@GBG ?NKMA>K K>LNEML : IARLB<:E ;:KKB>K MH
MA> =>@K:=:MBHG H? EB@GH<>EENEHL> 89
*B<KH:E@:> :K> @>G>K:EER <HFIHL>= H? <:K;HAR=K:M>L  EBIB=L
 :G= IKHM>BGL  89 +>O>KMA>E>LL MA> <HFIHLBMBHG H?
FB<KH:E@:> <:G O:KR PBMABG MA> LI><B>L :G= BG K>LIHGL> MH MA> <NEMBO:
MBHG <HG=BMBHGL 89 1A> F:CHK <:K;HAR=K:M> BG FB<KH:E@:E <>EE P:EE
BL <>EENEHL> PABE> LM:K<A BL MA> F:BG <:K;HAR=K:M> BG IE:LMB=L 89
*B<KH:E@:> A:OBG@ AB@A <HGM>GM H? EBIB=L :K> I:KMB<NE:KER NL>?NE ?HK
;BH=B>L>E IKH=N<MBHG PABE> <:K;HAR=K:M>L >GKB<A>= LI><B>L :K> :MMK:<
MBO> ?>>=LMH<DL ?HK ?>KF>GM:MBO> ;BH?N>EL 89 ,G> H? MA> IKHFBLBG@
<A:K:<M>KBLMB<L H? FB<KH:E@:> BL MA> :;L>G<> H? EB@GBG :L P>EE :L EB@GH<>E
ENEHLB< F:MKBQ BG MA> ;BHF:LL MA:M F:D> MA>F FHK> IKHFBLBG@ MA:G ) 
89 *B<KH:E@:> :K> :ELH IHM>GMB:E ?HK MA>BK >Q<>IMBHG:E <>EE P:EEL
<HFIHLBMBHG MA:M :K> >GKB<A>= PBMA IHERL:<<A:KB=>L 89 1A> MPH E:R
>K>= <>EE P:EEL H? FB<KH:E@:> <HGM:BG IHERL:<<A:KB=> F:MKBQ BG MA> >QM>K
G:E E:R>K LN<A :L I><MBG :@:K :E@BG:M> :G= :E@:>G:G PABE> MA> BGM>KG:E
E:R>K <HGM:BGL A>FB<>EENEHL> I><MBG :G= @ER<HIKHM>BG BG : FB<KHi;KBEL
F:MKBQ H? <>EENEHL> #B@  89 HFIHLBMBHG :G= LMKN<MNK> H? MA> <>EE
P:EE IKHOB=> KB@B=BMR :G= K><:E<BMK:G<> MH FB<KH:E@:E ;BHF:LL MA:M IK>
O>GML >??><MBO> ;BH=>@K:=:;BEBMR H? MABL ;BHF:LL <:M>@HKR 89
 /53'99 )54<+89/54 85;:+9
1A> <HGO>KLBHG H? ;BHF:LL BGMH ;BH?N>EL BL @>G>K:EER =HG> ;R MAK>>
=BLMBG<M KHNM>L G:F>ER MA>KFH<A>FB<:E AR=KHMA>KF:E :G= ;BH<A>FB<:E
KHNM>L #B@  PAB<A I:KMB<NE:KER O:KR BG M>KFL H? M><AGHEH@R :G= iG:E
IKH=N<M 89 1A>KFH<A>FB<:E <HGO>KLBHG H? ;BHF:LL BG<EN=>L ;HMA =B
K><M :G= BG=BK><M :IIKH:<A>L &G MA> =BK><M <HF;NLMBHG IKH<>LL ;BHF:LL
BL <HGO>KM>= BGMH A>:M :G= >E><MKB<BMR 1A> BG=BK><M IKH<>LL>L BG<EN=> IR
KHERLBL :G= @:LBi<:MBHG MA:M IKH=N<> ;BHHBEL :G= LRGMA>LBL @:L LRG@:L
, %p 89 0RG@:L MANL IKH=N<>= <:G ?NKMA>K ;> <HGO>KM>= BGMH ;BH
?N>EL LN<A :L ;BH>MA:GHE
%R=KHMA>KF:E I:MAP:R ;:LB<:EER NL>L LNI>K<KBMB<:E P:M>K :L MA>
F>=BNF ?HK <HGO>KMBG@ ;BHF:LL BGMH LN@:KL :G= LRG@:L 1A> ;BH<A>FB
<:E KHNM> BG<EN=>L LHF> LM>IL ?HK ;BHF:LL <HGO>KLBHG LN<A :L IK>MK>:M
F>GM AR=KHERLBL :G= ?>KF>GM:MBHG #>KF>GM:MBHG BL : <HFFHG :G= i
G:E LM>I PA>K> LHEN;E> LN@:KL H;M:BG>= BG MA> NILMK>:F IKH<>LL>L :K>
<HGO>KM>= BGMH ;BH?N>EL EBD> ;BH>MA:GHE ;BH;NM:GHE ;BH@:L :G= ;BH
AR=KH@>G #B@  EMAHN@A >:<A ;BHF:LL <HGO>KLBHG KHNM> A:L BML
Fig. 4. 0MKN<MNK> :G= <HFIHLBMBHG H? : MRIB<:E FB<KH:E@:E <>EE P:EE K>=K:PG ?KHF 89
UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
Fig. 5. 1AK>> F:CHK KHNM>L H? ;BHF:LL <HGO>KLBHG PBMA =B??>K>GM MRI>L H? ;BH?N>EL />
=K:PG ?KHF 89
HPG <A:EE>G@>L :G= IKHLI><ML ;BH<A>FB<:E I:MAP:R BL <HGLB=>K>= FHK>
><H?KB>G=ER ?>:LB;E> :G= <HLM>??><MBO>
 8+:8+':3+4: 5, (/53'99
&G MA> ;BH<A>FB<:E <HGO>KLBHG H? ;BHF:LL BGMH ;BH?N>EL IK>MK>:MF>GM
BL MA> <KN<B:E LM>I &M BL K>JNBK>= ?HK HO>K<HFBG@ MA> K><:E<BMK:G<> H? MA>
;BHF:LL 1A> K><:E<BMK:G<> H? MA> <>EE P:EE <HFIHG>GML BG )  BL IKBG
<BI:EER :MMKB;NM>= MH EB@GBG <HGM>GM <>EENEHL> <KRLM:EEBGBMR :G= :<<>LLB
;E> LNK?:<> :K>: 89 1A>K>?HK> MA> F:BG H;C><MBO> H? IK>MK>:MF>GM
BL MH K>FHO> HK :EM>K EB@GBG ?KHF MA> EB@GH<>EENEHLB< F:MKBQ K>=N<> MA>
<KRLM:EEBGBMR H? <>EENEHL> :G= >GE:K@> MA> LNK?:<> :K>: 89 0BG<> FB
<KH:E@:> =B??>K ?KHF )  BG <>EE P:EE LMKN<MNK> =N> MH A:OBG@ GH HK IHHK
EB@GBG =>EB@GBi<:MBHG BL GHM : <HG<>KG BG MA> IK>MK>:MF>GM H? MABL ;BH
F:LL <:M>@HKR %HP>O>K FB<KH:E@:E <>EE P:EE <HGLBLML H? <>EENEHL> :G=
I><MBG MA:M @BO> : KB@B= LMKN<MNK> :G= MANL K>LBLM :<<>LLB;BEBMR H? MA> ;BH
F:LL MH MA> AR=KHERMB< >GSRF>L 89 HGL>JN>GMER ;BH=>@K:=:;BEBMR H?
FB<KH:E@:E ;BHF:LL BL :ELH : F:CHK BLLN> BG MA> IKH=N<MBHG H? $ ;BH?N
>EL 1A> H;C><MBO>L H? IK>MK>:MBG@ MA> FB<KH:E@:> ;BHF:LL :K> MH =BLBGM>
@K:M> <>EE P:EE LMKN<MNK> :G= FH=B?R MA> LMKN<MNK> H? BGMK:<>EENE:K <:K;H
AR=K:M>L EBD> <>EENEHL> :G= LM:K<A 89
&G MA> I:LM MPH =><:=>L ;NG<A>L H? IK>MK>:MF>GM F>MAH=L A:O> ;>>G
BGO>LMB@:M>= >BMA>K LBFIE> HK M><AGHEH@B<:EER FHK> BGM>GLBO> PAB<A :K>
<E:LLBi>= :L IARLB<:E <A>FB<:E IARLB<H<A>FB<:E :G= ;BHEH@B<:E ":<A
MRI> H? IK>MK>:MF>GM F>MAH= <:G ;> =HG> ;R =B??>K>GM M><AGBJN>L MA:M
<:G >Q>KM O:KRBG@ >??><ML HG MA> ;BHF:LL 1:;E>  *HLM IARLB<:E :G=
<A>FB<:E IK>MK>:MF>GML :K> <HG=N<M>= ;R NLBG@ F><A:GB<:E <HFFBGN
MBHG BKK:=B:MBHG :<B= :ED:EB LM>:F >QIEHLBHG HK <HF;BG>= IKH<>LL>L
PAB<A K>JNBK> AB@A >G>K@R LM>:F :G= >E><MKB<BMR :G= <HKKHLBHG K>
LBLM:GM AB@AIK>LLNK> K>:<MHKL 89 &G :==BMBHG IKH=N<MBHG H? O:KBHNL
BGAB;BMHKL =NKBG@ IK>MK>:MF>GM PBMA MA> MK:=BMBHG:E :IIKH:<A>L LB@GB?B
<:GMER :??><M MA> RB>E= H? =HPGLMK>:F >GSRF:MB< AR=KHERLBL :G=HK ?>K
F>GM:MBHG &G ?:<M : LNLM:BG:;E> IK>MK>:MF>GM F>MAH= LAHNE= F>>M LHF>
<KBM>KB: LN<A :L  :EM>K:MBHG H? <>EENEHL> ?HK >?i<B>GM =HPGLMK>:F >GSR
F:MB< :MM:<D  FBGBFNF EHLL H? A>FB<>EENEHL>L :G= <>EENEHL>  @>G
>K:MBHG H? GH HK E>LL BGAB;BMHKL  K>JNBK>F>GM H? EHP >G>K@R  EHP
<HLML ?HK LBS> K>=N<MBHG H? ;BHF:LL :G= K>:<MHKL  GH HK EHP K>LB=N>L
?HKF:MBHG :G=  <HGLNFIMBHG H? EBMME> HK GH <A>FB<:E 89 &G MABL
K>@:K= - <HNE= A:O> FN<A IHM>GMB:E MH ;> <HFIEB>= PBMA FHLM H? MA>L>
LNLM:BG:;E> <KBM>KB: 89
 /525-/)'2 68+:8+':3+4:
- ;:LB<:EER >QIEHBML MA> G:MNK:E :;BEBMR H? LHF> FB<KHHK@:GBLFL MH
=>@K:=> EB@GBG BG )  HK <>EE P:EEL <HFIHG>GML <:K;HAR=K:M>L EBIB=L
:G= IKHM>BGL BG FB<KH:E@:> &G : MRIB<:E FB<KH;B:E IK>MK>:MF>GM IKH<>LL
<>EEL :K> :EEHP>= MH @KHP HG ;BHF:LL >BMA>K ;R MA> LN;F>K@>= <NEMNK>
89 HK LHEB=LM:M> <NEMBO:MBHG M><AGBJN>L 89 PAB<A MA>G FNEMBIER
:G= <:KKR HNM MA>BK F>M:;HEBLF ,? MA>L> MPH <NEMBO:MBHG M><AGBJN>L
LN;F>K@>= <NEMBO:MBHG BL M><AGB<:EER ?>:LB;E> ;NM BGBMB:E LHEB= EH:=BG@ K>
JNBK>= MH ;> EBFBM>= MH :KHNG=  ?HK ;>MM>K >?i<B>G<R PABE> LHEB=LM:M>
F>MAH= <:G NL> FHK> BGBMB:E LHEB=  89 - BG<EN=> : PB=>
K:G@> H? G:MNK:EER H<<NKKBG@ FB<KHHK@:GBLFL >BMA>K LBG@E> HK BG <H<NE
MNK> HK >GSRF> LRLM>FL INK> HK FBQMNK> <<HK=BG@ER - <:G ;> <:M>
@HKBS>= BGMH FB<KH;B:E IK>MK>:MF>GM ?NG@:E ;:<M>KB:E FB<KH;B:E <HGLHK
MB: :G= >GLBEBG@ :G= >GSRF:MB< IK>MK>:MF>GM 1A> F:CHK M><AGHEH@B<:E
:LI><ML :IIKHQBF:M> IK>MK>:MF>GM MBF> I>KBH= :=O:GM:@>L :G= =BL:=
O:GM:@>L H? >:<A :IIKH:<A :K> LNFF:KBS>= BG 1:;E> 
FHG@ MA> - :IIKH:<A>L ?NG@:E :G= >GLBEBG@ IK>MK>:MF>GML K>JNBK>
K>E:MBO>ER AB@A>K MBF> NI MH FHGMAL <HFI:K>= MH MA> MBF> K>JNBK>=
?HK ;R ;:<M>KB:E <HGLHKMB: :G= >GSRFB< IK>MK>:MF>GML %HP>O>K MBF>
K>JNBK>= ?HK :G >??><MBO> IK>MK>:MF>GM LHE>ER =>I>G=L HG MA> ;BHF:LL
LMKN<MNK> :G= <HFIHLBMBHG PA>K> IK>MK>:MF>GM H? )  FB@AM G>>= EHG@
MBF> MH ;K>:D =HPG BML EB@GH<>EENEHLB< F:MKBQ :G= K>FHO:E H? EB@GBG
L LM:M>= >:KEB>K 0><MBHG  MA> M:K@>M H? IK>MK>:MF>GM :ELH O:KB>=
;>MP>>G )  :G= FB<KH:E@:> LBG<> MA> ?HKF>K >QI><ML =>EB@GBi<:MBHG
PBMAHNM HK PBMA FBGBFNF EHLL H? AHEEH<>EENEHL> PABE> AR=KHERLBL H?
F:<KHFHE><NE>L BL MA> M:K@>M H? FB<KH:E@:> IK>MK>:MF>GM L : K>LNEM :G
BGM>K <HFI:KBLHG HG MA> IK>MK>:MF>GM RB>E= ;>MP>>G )  :G= FB<KH:E
@:> ;BHF:LL PHNE= GHM ;> K>:EBLMB< +>O>KMA>E>LL =>EB@GBi<:MBHG HK AR
=KHERMB< >?i<B>G<R GHM HGER O:KR =N> MH MA> IK>MK>:MF>GM :IIKH:<A :I
IEB>= ;NM :ELH BM <:G =B??>K :FHG@ MA> ;BHF:LL BG MA> L:F> <:M>@HKR >B
MA>K )  HK FB<KH:E@:> - H? )  BL F:BGER =HG> ;R ?NG@B PABE> >G
SRF:MB< HK AR=KHERMB< ;:<M>KB:E IK>MK>:MF>GM A:O> FHLMER ;>>G K>IHKM>=
?HK FB<KH:E@:> IK>MK>:MF>GM
 "+).45+)5453/) )536'8/954 5,   '4*  (/5,;+29
-KH=N<MBHG H? $ ;BH?N>EL ?KHF FB<KH:E@:> BL M><AGH><HGHFB<:EER
FHK> ?>:LB;E> MA:G MA> IKH=N<MBHG H? $ :G= $ ;BH?N>EL 89 *B
<KH:E@:> :K> IKHFBLBG@ HO>K $ :G= $ ?>>=LMH<DL ?HK MA> GHGK>JNBK>
F>GM H? :K:;E> E:G= ?>:LB;BEBMR H? K><R<EBG@ MA> GNMKB>GML F:QBFNF ;BH
F:LL NMBEBS:MBHG EHP HI>K:MBHG:E <HLML E>LL >G>K@R <HGLNFIMBHG :G= :
LNLM:BG:;E> ;BH?N>EL IKH=N<MBHG 89 #HK BGLM:G<> FB<KH:E@:> <:G
IKH=N<> FHK> MA:G  MBF>L AB@A>K ;BHF:LL MA:G MA> ;BHF:LL H;M:BG>=
?KHF MA> ?:LM>LM @KHPBG@ M>KK>LMKB:E IE:GM LPBM<A @K:LL 89 GHMA>K
>Q<>EE>GM M><AGB<:E ?>:LB;BEBMR BG MA> IKH=N<MBHG H? FB<KH:E@:> ;BHF:LL BL
MA> :;BEBMR MH @KHP MA>F BG P:LM>P:M>K 89 *HK>HO>K LHF> A:EHIABEB<
A:EHMHE>K:GM :G= A:EH:=:IM>= FB<KH:E@:> LI><B>L A:O> K><>GMER ;>>G K>
IHKM>= ?HK LNLM:BG:;E> ;BH?N>E IKH=N<MBHG MA:M <:G ;> <NEMBO:M>= BG F:
KBG> HK L:EBG> P:M>K 89
!>LIBM> MA> ;KB@AM IKHLI><ML H? FB<KH:E@:> BG MA> IKH=N<MBHG H? ;BH
?N>EL :G= ;BHK>iG>KR BM BL LMBEE FHLMER <HGiG>= MH MA> K>L>:K<A E>O>E >Q
<>IM : ?>P K>IHKML HG BML <HFF>K<B:EBS:MBHG 89 HFI:K>= MH $
;BH?N>EL K>L>:K<A :<AB>O>F>GML :G= <HFF>K<B:E >QIEHBM:MBHG H? $ ;BH
?N>EL BL K>E:MBO>ER F:MNK> ;NM BM BL GHM M><AGH><HGHFB<:EER <HFI>MBMBO>
MH $ ;BH?N>EL 1A>K>?HK> M><AGH><HGHFB< BFIKHO>F>GML :K> LMBEE K>
JNBK>= ?HK ;HMA $ :G= $ ;BH?N>EL MH K>:<A MH MA> L:MBL?:<MHKR E>O>E H?
BG=NLMKB:E :K>G: &G MABL K>@:K= >QIEHBM:MBHG H? ;BHEH@B<:E IK>MK>:MF>GM
BG MA> @>G>K:MBHG H? $ :G= $ ;BH?N>EL <HNE= ;KBG@ : ;K>:DMAKHN@A ?HK
MA>L> ;BH?N>EL
UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
Table 1
"QIEHK:MBHG H? O:KBHNL F>MAH=L ?HK IK>MK>:MF>GM H? EB@GH<>EENEHLB< :G= FB<KH:E@:> ;BHF:LL 89
-K>MK>:MF>GM
F>MAH=L IIKH:<A>L 1BF>
*:CHK>m><ML
HG;BHF:LL
LMKN<MNK> =O:GM:@>L !BL:=O:GM:@>L
!>O>EHIF>GM
LM:@>
*><A:GB<:E
-ARLB<:E -K><HG=BMBHGBG@MH  FF LBS>
ABIIBG@MH  FF LBS>
$KBG=BG@:G=FBEEBG@MH  FF LBS>
*><A:GB<:E<HFFBGNMBHGMHiG>LBS>
?>P
FBGNM>L
 FBG
"QM>G=>=
LNK?:<>:K>:
!><K>:L>=
<KRLM:EEBGBMR
H?<>EENEHL>
"GA:G<>=
;NED=>GLBMR
&FIKHO>=
:<<>LLB;BEBMR
MH>GSRF>L
&G<K>:L>=
IHKHLBMR
"GA:G<>=
jHP IKHI>K
MB>L
":LRA:G=EBG@
&FIKHO>= F:LL :G=
A>:M MK:GL?>K BG MA>
LN;L>JN>GM AR=KHERLBL
:G=?>KF>GM:MBHG
%B@AAR=KHERLBLRB>E=
%B@A >G>K@R
BGINM
+H EB@GBG K>
FHO:E
"<HGHFB
<:EER GHM
?>:LB;E>
HFF>K<B:EER
IKHO>G
M><AGHEH@R
&KK:=B:MBHG
-ARLB<:E $:FF:BKK:=B:MBHG
"E><MKHG;>:FBKK:=B:MBHG
*B<KHP:O>BKK:=B:MBHG
2EMK:LHGB<MK>:MF>GM
*BGNM>LMH
AHNKL "QM>G=>=
LNK?:<>:K>:
!><K>:L>=
<KRLM:EEBGBMR
H?<>EENEHL>
0HEN;BEBS:
MBHG H?
A>FB<>EEN
EHL>
-:KMB:E
=>@K:=:MBHG
H?EB@GBG
!><K>:L> BG
MA> FHE><N
E:KP>B@AM
&FIKHO>= =HPGLMK>:F
>GSRF:MB< AR=KHERLBL
K:M>
%B@A>G>K@R>k<B>G<R
"QI>GLBO>
0EHP :EM>K
:MBHGK:M>
+HM ><H
?KB>G=ER
HFF>K<B:EER
IKHO>G
M><AGHEH@R
<B= A>FB<:E -K>MK>:MF>GM PBMA BGHK@:GB< :<B=L LN<A :L
%p0,p % E %p-,p %+,p PA>K> BL %p0,p
FHLMERNL>=
,K@:GB< :<B=L EBD> ?NF:KB< :G= F:E>B< :<B=L F:R
:ELH;>NL>=
<B= <HG<>GMK:MBHG BL ;>EHP  BG =BENM> :<B=
:G=  BG<HG<>GMK:M>=:<B=MK>:MF>GML
1>FI>K:MNK> K:G@>L ?KHF  MH  ` BG =BENM>
:<B= :G=  ` BG <HG<>GMK:M>= :<B= MK>:M
F>GML
 FBG "QM>G=>=
LNK?:<>:K>:
/>FHO:E H?
A>FB<>EEN
EHL>
EM>K>=
EB@GBG LMKN<
MNK>
"GA:G<>=
=B@>LMB;BEBMR
%B@AK>:<MBHGK:M>
4>EELMN=B>= M><A
GBJN>
)HP EB@GBG
LHEN;BEBS:
MBHG
%B@A BG
AB;BMHK ?HK
F:MBHG
HKKHLBHG H?
>JNBIF>GM
K>:<MHK
/>JNBK>
F>GM H?
G>NMK:EBS:
MBHG
!>FHGLMK:MBHG
IE:GM;R
'>GGBG@L20
ED:EB A>FB<:E +:,% (,% :G= :,%p :K> PB=>ER NL>= ?HK :E
D:EBG>IK>MK>:MF>GM
0>O>K:E
FBGNM>LMH
AHNKL
"QM>G=>=
LNK?:<>:K>:
0HEN;BEBS:
MBHG H?
A>FB<>EEN
EHL>
!>@K:=:MBHG
H?EB@GBG
/>JNBK>F>GM H? EHP
M>FI>K:MNK> :G= IK>L
LNK>
&FIKHO>= =HPGLMK>:F
AR=KHERLBL
$HH= ?HK EHP EB@GBG
<HGM:BGBG@;BHF:LL
/>E:MBO>ER
EHG@ K>:<
MBHGMBF>
!Bk<NEMB>L
BG G>NMK:E
BS:MBHG
):;HK:MHKRL<:E>
UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
Table 1 54:/4;+*
-K>MK>:MF>GM
F>MAH=L IIKH:<A>L 1BF>
*:CHK>m><ML
HG;BHF:LL
LMKN<MNK> =O:GM:@>L !BL:=O:GM:@>L
!>O>EHIF>GM
LM:@>
,SHGHERLBL
 A>FB<:E "QIHLNK> H? ;BHF:LL MH HSHG> @:L :M : jHP K:M>
H?:KHNG=  FEFBG
 FBG 0>E><MBO>
EB@GBG LHEN
;BEBS:MBHG
)HP HK BGAB;BMHK ?HK
F:MBHG
/HHF M>FI>K:MNK>
:G= :MFHLIA>KB< IK>L
LNK>
%B@A <HLML
?HK HGLBM>
HSHG> IKH
=N<MBHG :G=
NL>
0:?>MR BLLN>L
?HK j:FF:
;BEBMR :G=
MHQB<BMR H?
HSHG>
):;HK:MHKRL<:E>
&HGB<EBJNB=L&)L
 A>FB<:E "QIHLNK> H? ;BHF:LL MH &)L MA:M :K> EBJNB=L :M
:F;B>GM M>FI>K:MNK> :G= K>F:BG EBJNB= NI MH :
<>KM:BGK:G@>H?M>FI>K:MNK>L:MNIMH  `
 FBG "GE:K@>=
LNK?:<>:K>:
0HEN;BEBS:
MBHG H? <>EEN
EHL> :G=
A>FB<>EEN
EHL>
)B@GBG K>
FHO:E
&)L :K> K><R<E:;E> :G=
K>NL:;E>
)HP >G>K@R <HGLNFI
MBHG
%B@A;BHF:LLEH:=
%B@A <HLML
H?&)L
/>JNBK>
F>GM H? &)
K><HO>KR
0MBEEBGMA>
IKBF:KRLM:@>H?
K>L>:K<A
,K@:GHLHEO
 A>FB<:E "QIHLNK> H? ;BHF:LL MH : FBQMNK> H? :G HK@:GB<
EBJNB=:G=P:M>KBGK:MBH
*BG>K:E :<B= F:R ;> NL>= :L <:M:ERLM ?HK =><K>:L
BG@ IKH<>LL M>FI>K:MNK>L HK BFIKHOBG@ EB@GBG K>
FHO:E
 FBG 0HEN;BEBS:
MBHG H?
A>FB<>EEN
EHL> :G=
EB@GBG
/>FHO:E H?
<>EENEHL>
?KHF ;BH
F:LL BG
LHEB=?HKF
)HP M>FI>K:MNK> :G=
IK>LLNK>
+>NMK:E I% <HG=BMBHG
)HP<>EENEHL>EHLL
-NK><>EENEHL>RB>E=
"m><MBO> ?HK AB@A
EB@GBG <HGM:BGBG@ ;BH
F:LL
+>>= MH K>
FHO> LHE
O>GML
%B@A<HLML
&GAB;BMHK
@>G>K:MBHG
):;HK:MHKRL<:E>
0M>:F>QIEHLBHG
-ARLB<H<A>FB<:E &G<EN=>L:NMHAR=KHERLBL:G=:G>QIEHLBHGLM>I
.NB<D A>:MBG@ ;R AB@AIK>LLNK> L:MNK:M>= LM>:F
:M  ` :G=  :MF
:G ;> =HG> PBMA HK PBMAHNM :==BMBHG H? :G :<B=
:L<:M:ERLM
0>O>K:E
FBGNM>L
:KHNG=
 FBG
"QM>G=>=
LNK?:<>:K>:
0HEN;BEBS:
MBHG H?
A>FB<>EEN
EHL>
0HEN;BEBS:
MBHG H?
EB@GBG
EM>K:MBHG
H? EB@GBG
LMKN<MNK>
+H K><R<EBG@ HK >GOB
KHGF>GM:E<HLML
0AHKM=NK:MBHG
)HP >G>K@R K>JNBK>
F>GM
0N@:K EHLL
=NKBG@ IHLM
AR=KHERLBL
IKH<>LL
&GAB;BMHK
?HKF:MBHG
HFF>K<B:EER
:=:IM>=
)BJNB=AHMP:M>K
-ARLB<H<A>FB<:E 2L:@> H? P:M>K :L MA> K>:<MBHG F>=BNF :M AB@A
M>FI>K:MNK>L  `  :G=IK>LLNK>
 FBG "QM>G=>=
LNK?:<>:K>:
0HEN;BEBS:
MBHG H?
A>FB<>EEN
EHL>
0HEN;BEBS:
MBHG H?
EB@GBG
+H K>JNBK>F>GM H? <:M
:ERLMLHK<A>FB<:EL
+H HK EBMME> BGAB;BMHK
?HKF:MBHG
%B@A K><HO>KR H? LN@
:KL
%B@A P:M>K
K>JNBK>F>GM
%B@A >G>K@R
BGINM
%B@A<HLML
)HP RB>E= H?
?>KF>GM:;E>
LN@:KL
=:IM>=MHIBEHM
L<:E>
4>MHQB=:MBHG
-ARLB<H<A>FB<:E "QIHLNK> H? ;BHF:LL MH AB@A M>FI>K:MNK>
 `  :G= IK>LLNK> NI MH  *-: NL
BG@HQR@>GHK:BK
 FBG "QM>G=>=
LNK?:<>:K>:
)B@GBG =>
<HFIHLBMBHG
/>=N<>=
<KRLM:EEBGBMR
H?<>EENEHL>
*H=>K:M><:IBM:E<HLML
$HH=EB@GBGK>FHO:E
$HH= <:K;HAR=K:M> K>
<HO>KR
&FIKHO>= >GSRF:MB<
=B@>LMB;BEBMR
%B@A >G>K@R
BGINM
)HP A>FB
<>EENEHL> K>
<HO>KR
/>JNBK>
F>GM H? >Q
I>GLBO> F:
M>KB:EL ?HK
K>:<MHK
=:IM>=BG
=>FHGLMK:MBHG
IE:GM;RBH$:LHE
:G=0MK:N;BG@
UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
Table 1 54:/4;+*
-K>MK>:MF>GM
F>MAH=L IIKH:<A>L 1BF>
*:CHK>m><ML
HG;BHF:LL
LMKN<MNK> =O:GM:@>L !BL:=O:GM:@>L
!>O>EHIF>GM
LM:@>
0NI>K<KBMB<:EjNB=
-ARLB<H<A>FB<:E 2L:@> H? LHEO>GM :M MA> M>FI>K:MNK> :G= IK>L
LNK>:;HO>MA><KBMB<:EIHBGM
-K>MK>:MF>GM PBMA LNI>K<KBMB<:E ,p BL PB=>ER
NL>= ?HK BML FH=>K:M> <KBMB<:E M>FI>K:MNK> :G=
IK>LLNK>
 FBG &G<K>:L>=
LNK?:<>:K>:
"m><MBO>
EB@GBG K>
FHO:E
&G<K>:L>=
<>EENEHL> =B
@>LMB;BEBMR
%B@ALHEB=EH:=
)HPM>FI>K:MNK>
)HP LN@:K =>@K:=:MBHG
*BGBFNF HI>K:MBHG:E
<HLML
1HM:E NMBEB
MB>L <HLML
:K>E:K@>K
,NMINM BL :?
?><M>= ;R
LHF> ?:<MHKL
):;HK:MHKRL<:E>
FFHGB:i;>K
>QIEHLBHG#"5
-ARLB<H<A>FB<:E
"QIHLNK> H? ;BHF:LL MH EBJNB= :FFHGB: :M
 ` :G= IK>LLNK> FHK> MA:G  *-: ?HK
KHN@AER  FBG PAB<A MA>G ?HEEHPL : K:IB= =>
<HFIK>LLBHG
 FBG "QM>G=>=
LNK?:<>:K>:
0HEN;BEBS:
MBHG H? <>EEN
EHL> :G=
A>FB<>EEN
EHL>
/>FHO:E H?
EB@GBG :G=
:EM>K>=
EB@GBG LMKN<
MNK>
3>KR >m><MBO> ?HK EHP
EB@GBG <HGM:BGBG@ ;BH
F:LL
%B@A <HLML
?HK :FFH
GB: :G=
>JNBIF>GM
HFF>K<B:EER
:=:IM>=;R
!N-HGM
BHEH@B<:E
IK>MK>:MF>GM NEMBO:MBHG H? EB@GBG =>@K:=BG@ ?NG@B :G= ;:<M>
KB: BG ;BHF:LL :M MA> HIMBFNF M>FI>K:MNK> :G=
<NEMNK:E <HG=BMBHGL H? MA> K>LI><MBO> FB<KHHK
@:GBLFL
P>>DL !>EB@GBi<:
MBHG H? ;BH
F:LL
0MKN<MNK:E
:EM>K:MBHG H?
<>EENEHL>
:G= A>FB
<>EENEHL>
)HP<HLM
"<H?KB>G=ER
+H K>E>:L> H? MHQB<
<HFIHNG=L
+H>lN>GM@>G>K:MBHG
+H BGAB;BMHK ?HKF:MBHG
)HG@ BG<N
;:MBHGMBF>
0EHP K:M> H?
=>EB@GBi<:
MBHG
:K;HAR
=K:M>EHLL
/BLD ?HK
A>:EMA A:S
:K=
=:IM>=MHIBEHM
L<:E>
*B<KH:E@:E ;BHF:LL <:G ;> IK>MK>:M>= ;BHEH@B<:EER NLBG@ AR=KHERMB<
>GSRF>L INKBi>= HK FBQMNK> H? >GSRF>L HK FB<KHHK@:GBLFL A:OBG@ AR
=KHERMB< :<MBOBMB>L !>I>G=BG@ HG MA> ;BH:@>GML NL>= MABL IK>MK>:MF>GM
<:G K>LNEM BG MA> O:KRBG@ :FHNGML H? =>IHERF>KBS:MBHG H? MA> ;BHIHER
F>KL <>EENEHL> LM:K<A EBIB= :G= IKHM>BG PAB<A IKHOB=>L LBFIE> FHE>
<NE>L ?HK ?NKMA>K =HPGLMK>:F IKH<>LLBG@ +- :G>KH;B< =B@>LMBHG HK ?>K
F>GM:MBHG 89 ,G MA> HMA>K A:G= - H? )  F:BGER =BLBGM>@K:M> MA>
EB@GH<>EENEHLB< F:MKBQ K>FHO> EB@GBG ?KHF BM :G= K>E>:L> AHEEH<>EENEHL>
PBMAHNM AR=KHERSBG@ MA> IHERL:<<A:KB=>L "O>GMN:EER : ?NKMA>K AR=KHE
RLBL LM>I BL G><>LL:KR ?HK MA> IK>MK>:M>= )  MH H;M:BG LBFIE> LN@:KL
?KHF <>EENEHL> :G=HK A>FB<>EENEHL> 1A>K>?HK> ;BHEH@B<:E IK>MK>:MF>GM
H? FB<KH:E@:> :L P>EE :L ;BH?N>E IKH=N<MBHG ?KHF MABL ;BHF:LL <:M>@HKR
:K> M><AGH><HGHFB<:EER FHK> ?>:LB;E> MA:G MAHL> H? ) 
2G?HKMNG:M>ER MA> G>M IKH=N<MBHG <HLML H?  $ ;BH?N>EL BL ?HNG= MH ;>
AB@A>K MA:G MA> <HLML H? $ :G=  $ >MA:GHE #HK >Q:FIE> MA> <HLML ?HK
<HKG >MA:GHE $ LPBM<A@K:LL >MA:GHE $ FBL<:GMANL >MA:GHE $
:G= <HKG LMHO>K >MA:GHE $ P>K> >LMBF:M>= MH ;>    :G=
 @:EEHG K>LI><MBO>ER PABE> MA> <HLM ?HK :E@:E ;BH=B>L>E P:L K>
IHKM>= MH ;> :KHNG=  @:EEHG 89 EMAHN@A $ ;BH?N>EL :K> FHK>
><HGHFB< IKH=N<MBHG H? MABL ;BH?N>E BL <HGiG>= MH HGER : ?>P <HNGMKB>L
EBD> 20 K:SBE :G= LHF> "NKHI>:G <HNGMKB>L *HK>HO>K MA> <AHB<>
H? ?>>=LMH<DL ?HK ;BH?N>E IKH=N<MBHG BG :GR <HNGMKR K>ER HG LHF> ?:<
MHKL BG<EN=BG@ MA> @>H@K:IAB< EH<:MBHG ><HGHFB< <HG=BMBHGL :G= =>
F:G= ?HK ?HH=?N>EL H? MA:M I:KMB<NE:K <HNGMKR HGLB=>KBG@ MA>L> ?:<MHKL
$ ;BH?N>EL @>G>K:EER AHE= FHK> IHM>GMB:E HO>K $ :G= $ ;BH?N>EL >O>G
MAHN@A FHK> =>O>EHIF>GML :K> LMBEE K>JNBK>= ?HK @>G>K:MBG@ <HLM>??><
MBO> ;BH?N>EL ?KHF FB<KH:E@:> 89
3. Approaches of biological pretreatment
 /525-/)'2 68+:8+':3+4: (? ,;4-/
#NG@:E IK>MK>:MF>GML :K> EBFBM>= MH )  :G= GH K>IHKM <:G ;> ?HNG=
MBEE GHP MA:M NL>= ?NG@:E <>EEL =BK><MER ?HK IK>MK>:MF>GM H? FB<KH:E@:>
;BHF:LL 89 EMAHN@A : LMN=R P:L K><>GMER K>IHKM>= BM NL>= HGER
<KN=> ;KHMA H? "8'3+:+9 <+89/)5258 <HGM:BGBG@ FBQMNK>L H? ?NG@:E >GSRF>L
:G= GHM MA> EBOBG@ <>EEL H? MA> ?NG@NL 89 0HF> F>F;>KL H? :LB=BHFR
<HM: :K> I:KMB<NE:KER K>GHPG>= ?HK MA>BK EB@GBG=>@K:=BG@ <:I:;BEBMR MA:M
A:O> EHG@ ;>>G NL>= ?HK MA> IK>MK>:MF>GM H? )  1A> EB@GBG =><HFIHL
BG@ ?NG@B :K> <E:LLBi>= BGMH MAK>> ><HEH@B<:E @KHNIL LN<A :L PABM>KHM
;KHPGKHM :G= LH?MKHM ?NG@B 4/# /# :G= 0/# K>LI><MBO>ER 4/#
:G= /# BG<EN=> MA> F>F;>KL H? ;:LB=BHFR<>M>L PABE> 0/# @KHNI <HG
LBLML FHLMER H? :L<HFR<>M>L
 @HH= GNF;>K H? 4/# <:G =>@K:=> EB@GBG <HFIE>M>ER ;R HQB
=BSBG@ EB@GBG FHE><NE>L 89 PA>K>:L EB@GBG ;K>:D=HPG ;R 0/# BL
LEHP :G= BG<HFIE>M> 89 *HK>HO>K 0/# =>@K:=> IHERL:<<A:KB=> ?K:<
MBHGL H? ;BHF:LL ?:LM>K MA:G BML EB@GBG ;K>:D=HPG &G :G >Q<>IMBHG
'*56.58' LI P:L K>IHKM>= MH =>@K:=> EB@GBG FHK> K:IB=ER MA:G MA>
<:K;HAR=K:M>L 89 &G MA> I:LM R>:KL : ?>P 0/# A:O> ;>>G K>IHKM>=
?HK - PAB<A BG<EN=> 96+8-/22;9 4/-+8+4/)/22/;3 ).8?95-+4;3 :G= "8/
).5*+83' 8++9+/ 89 /# IK>?>K>GMB:EER =>@K:=> AHEEH<>EENEHL> K:MA>K
MA:G EB@GBG 89 %HP>O>K MA>L> ?NG@B F:R :ELH I:KMB:EER ;K>:D=HPG
MA> EB@GBG FHE><NE>L MAKHN@A #>GMHGL K>:<MBHGL MA>K>;R ?:<BEBM:MBG@
MA> :<<>LL H? AR=KHERMB< >GSRF>L =NKBG@ =HPGLMK>:F AR=KHERLBL H? MA>
IK>MK>:M>= ;BHF:LL 89 /# NL>= ?HK IK>MK>:MF>GM H? ;BHF:LL BG
<EN=>= 25+56.?22;3 :8'(+;3 89 59:/' 62')+4:' 89 54/56.58'
6;:+'4' '4* 59:/' 62')+4:' 89 FHG@ MA> EB@GBG=>@K:=BG@ ?NG@B
4/#L :K> :II>:K>= MH ;> IK>=HFBG:GM ?HK IHLL>LLBG@ >?i<B>GM EB@GBG
=>@K:=BG@ <:I:;BEBMR &G :==BMBHG 4/# <:G :ELH M:K@>M <>EENEHL> :G=
BG<K>:L> >GSRF:MB< =B@>LMB;BEBMR H? MABL IHERL:<<A:
UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
Table 2
1><AGHEH@B<:E :IIKH:<A>L H? ;BHEH@B<:E IK>MK>:MF>GM
IIKH:<A *:CHKM><AGHEH@R
-K>MK>:MF>GM
MBF> =O:GM:@>L !BL:=O:GM:@>L />F:KDL />?
#NG@:E
IK>MK>:MF>GM "QIHLNK> H? ;BHF:LL MH BG=BOB=N:E ?NG
@:E LI><B>L F:BGER ;:LB=BHFR<>M>L
?NG@B NLBG@ >BMA>K LN;F>K@>= HK LHEB=
LM:M><NEMNK>M><AGBJN>
4>>DLMH
FHGMAL )HPIK>MK>:MF>GM<HLML
)>LL K>:@>GM <HGLNFIMBHG
)>LL>G>K@RK>JNBK>F>GM
+H HK E>LL P:LM> @>G>K:
MBHG
)HP <HLML ?HK =HPG
LMK>:FK><HO>KR
+H HK E>LL BGAB;BMHKL ?HK
F:MBHG
)HLL H? <:K;H
AR=K:M>L
)HG@ IK>MK>:M
F>GMMBF>
"k<B>GM?HKEB@GBG
K>FHO:E?KHF) ;NM
EHG@MBF>:G=EHLLH?
AHEEH<>EENEHL>PHNE=;>
MA>F:CHK;HMME>G><DL
89
:<M>KB:E
IK>MK>:MF>GM "QIHLNK> H? ;BHF:LL MH EB@GBG =>@K:=
BG@ )  HK AR=KHERMB< FB<KH:E@:>
;:<M>KB:
0>O>K:E=:RL *HK> K:IB= @KHPMA MA:G
?NG@B
/>E:MBO>ER LAHKM>K IK>
MK>:MF>GMMBF>
":LB>K @>G>MB< F:GBINE:
MBHGMA:G?NG@B
*HK> <HLM>m><MBO> MA:G
?NG@B
%B@A>K :=:IM:;BEBMR MA:G
?NG@B
+H HK E>LL BGAB;BMHKL ?HK
F:MBHG
/>E:MBO>ER
EHP>K EB@GBG
=>@K:=BG@ >k
<B>G<R <HF
I:K>=MH?NG@B
-KHFBLBG@?HK<HLM
>m><MBO>IK>MK>:MF>GM
I:KMB<NE:KER?HK
FB<KH:E@:>;BHF:LL
89
-K>MK>:MF>GM
;RFB<KH;B:E
<HGLHKMB:
"QIEHBM:MBHG H? <H<NEMNK> LRLM>FL
<HGLBLMBG@ H? ?NG@:E?NG@:E HK ;:<M>K
B:E;:<M>KB:EHK;:<M>KB:E?NG@:E
0>O>K:E=:RL />E:MBO>ER EHP>K BG<N;:
MBHG MBF> <HFI:K>= MH
?NG@:EIK>MK>:MF>GM
HFIE>Q FB<KH;B:E <HF
FNGBMR :<<>E>K:M> HK
@:GB< AR=KHERLBL PAB<A BG
MNKG ?:<BEBM:M> MA> :<<>LLB
;BEBMR H? >GSRF>L BG EB@GH
<>EENEHL>L
HGMBGNHNL :G= LM:;E>
@>G>K:MBHG H? F>M:;HEBM>L
K>JNBK>= ?HK EB@GBG ;K>:D
=HPG
"GA:G<>= IKH=N<MBOBMR
:G=;BHF:LLNIM:D>
&G=BOB=N:E
LMK:BGL F:R
?:<> =Bk<NEMB>L
BG :=:IMBG@ MH
MA> >GOBKHG
F>GM:E jN<MN:
MBHGL
HNE=;>IKHFBLBG@
IK>MK>:MF>GM?HK
FB<KH:E@:>BG<EN=BG@
FBQMNK>H?AR=KHERMB<
;:<M>KB:ELMK:BGL
89
"GSRF:MB<
IK>MK>:MF>GM 2MBEBS:MBHG H? INKBi>= HK <KN=> >G
SRF>L LBG@E> HK >GSRF> <H<DM:BEL
EB@GBGHERMB<HKAR=KHERMB<HKFBQ>=
%HNKLMH:
?>P=:RL />E:MBO>ER EHP>K BG<N;:
MBHG MBF> <HFI:K>= MH
?NG@:E :G= ;:<M>KB:E IK>
MK>:MF>GM
%R=KHERLBL H? F:<KHFHE>
<NE>L MA:M ?:<BEBM:M>L ?NK
MA>K <HGO>KLBHG IKH<>LL
I:KMB<NE:KER =NKBG@ ;BH@:L
IKH=N<MBHG
-KH=N<MBHG :G=
INKBi<:MBHG H?
>GSRF>L F:R
;><HLMER
-HHK LM:;BEBMR
H? EB@GBGHERMB<
>GSRF>L BG BG
=NLMKB:E
IKH<>LL>L
1A>FHLM>k<B>GM
;BHEH@B<:EIK>MK>:MF>GM
I:KMB<NE:KER?HK
FB<KH:E@:>
89
Table 2 54:/4;+*
IIKH:<A *:CHKM><AGHEH@R
-K>MK>:MF>GM
MBF> =O:GM:@>L !BL:=O:GM:@>L />F:KDL />?
-K>MK>:MF>GM
;R>GLBEBG@ 0BFNEM:G>HNL LMHK:@> :G= IK>MK>:M
F>GM H? ;BHF:LL NLBG@ O:KBHNL ;BHEH@
B<:E LRLM>FL LN<A :L FBQMNK>L H? E:<MB<
:<B= ;:<M>KB: ) FBQMNK> H? )
:G=AR=KHERMB<>GSRF>L
4>>DLMH
FHGMAL 4B=>ER NL>= ?HK <KHI LMHK
:@>
:G ;> <:KKB>= HNM NG=>K
FH=>K:M> >GOBKHGF>GM:E
<HG=BMBHGL
)>LL HK GH P:LM>L @>G>K:
MBHG
)HP=HPGLMK>:F<HLML
)HG@ BG<N;:
MBHGMBF>
)HLL H? HK@:GB<
F:MM>K F:BGER
<>EENEHL> :G=
A>FB<>EENEHL>L
-KHFBLBG@?HK
LBFNEM:G>HNLLMHK:@>
:G=IK>MK>:MF>GMH?
) :G=FB<KH:E@:>
;BHF:LL
89
KB=> 89 %HP>O>K HG> H? MA> =K:P;:<DL H? LN<A :<MBOBMR BL MA> GHGL>
E><MBO> IK>MK>:MF>GM MA:M <:NL>L EHLL H? <>EENEHL> =NKBG@ IK>MK>:MF>GM
0>E><MBO> :G= GHGL>E><MBO> EB@GBG ;K>:D=HPG ;R 4/#L =>I>G= HG
MA>BK <:I:;BEBMR MH IKH=N<> <:K;HAR=K:M> =>@K:=BG@ AR=KHERMB< >G
SRF>L PBMA MA> L><K>MBHG H? >QMK:<>EENE:K EB@GBGHERMB< >GSRF>L 0HF>
4/#L <:G IKH=N<> HGER EB@GBGHERMB< >GSRF>L PBMAHNM IKH=N<BG@ :GR
AR=KHERMB< QRE:G:L>L :G= <>EENE:L>L PAB<A BG<EN=> !:85(/2;8;9 5.9./
3'+.'4+85).'+:+ ).8?959658/;3 :G= "8'3+:+9 <+89/)5258 89 0>O>K:E
DGHPG 4/#L +- 2+;85:;9 59:8+':;9 <:G :ELH IKH=N<> AR=KHERMB< >G
SRF>L 89 %HP>O>K  59:8+':;9 <:G =>@K:=> EB@GBG L>E><MBO>ER
PBMAHNM =><HFIHLBG@ <>EENEHL> :G= A>FB<>EENEHL> 89 &M A:L BG=B
<:M>= MA:M L>E><MBO> EB@GBG K>FHO:E FB@AM ;> K>E:M>= GHM HGER MH MA>
IKH=N<MBHG H? AR=KHE:L>L ;NM :ELH MH LHF> HMA>K ?:<MHKL LN<A :L MA>
UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
IK>MK>:MF>GM <HG=BMBHGL 1A> >?i<B>G<R H? ;BHEH@B<:E =>EB@GBi<:MBHG <:G
;> =>M>KFBG>= ;R F>:LNKBG@ MA> L>E><MBOBMR O:EN> ?HK EB@GBG ;K>:D=HPG
PAB<A BL =>iG>= :L MA> K:MBH H? EB@GBG EHLL MH <>EENEHL> EHLL 89 #NG@:E
LMK:BGL LAHPBG@ : EHP L>E><MBOBMR O:EN>   LAHNE= GHM ;> <HGLB=>K>=
?HK ;BHEH@B<:E =>EB@GBi<:MBHG 89
1A> >?i<B>G<R :L P>EE :L HNM<HF>L H? )  IK>MK>:MF>GM ;R 4/#L :K>
IKBF:KBER :??><M>= ;R MA> ?NG@:E LI><B>L :G= G:MNK> H? ;BHF:LL 1:;E>
 &G :==BMBHG MA> :FHNGM H? =>EB@GBi<:MBHG =>I>G=L HG MA> JN:GMBMR H?
EB@GBG BG MA> ;BHF:LL PA>K> MA> EHP>LM EB@GBG <HGM:BGBG@ ;BHF:LL F:R
LAHP MA> AB@A>LM EB@GBG K>FHO:E B? HMA>K ?:<MHKL K>F:BG <HGLM:GM HK HNM
H? <HGLB=>K:MBHG ,G MA> HMA>K A:G= :FHNGML H? EB@GBG K>FHO:E :G= MA>
MBF> K>JNBK>= ?HK IK>MK>:MF>GM F:R :ELH O:KR =>I>G=BG@ HG MA> G:MNK>
H? ;BHF:LL #HK >Q:FIE> PHH=R ;BHF:LL K>JNBK>L K>E:MBO>ER AB@A>K BG<N
;:MBHG MBF> PBMA >O>G : EHP>K :FHNGM H? EB@GBG K>FHO:E BG <HFI:KBLHG
PBMA MA> :@KB<NEMNK:E K>LB=N>L HK @K:LL>L 1:;E>  89 1A> K>:LHG ;>
ABG= MABL <HNE= ;> MA> IK>L>G<> H? K>E:MBO>ER AB@A>K :FHNGML H? =>@K:=
:;E> <HFIHNG=L BG MA> K>LB=N>L MA:G BG MA> PHH=L 89
 /525-/)'2 68+:8+':3+4: (? ('):+8/'
2MBEBS:MBHG H? ;:<M>KB: BG - <HNE= ;> FHK> IKHFBLBG@ ?HK K>=N<
BG@ MA> IK>MK>:MF>GM MBF> :L MA> @KHPMA K:M> :G= F>M:;HEB< :<MBOBMR
H? ;:<M>KB: :K> ?:LM>K MA:G MAHL> H? ?NG@:E LI><B>L %HP>O>K EB@GBG
=>@K:=:MBHG ;R MA> ;:<M>KB:E LMK:BGL BL GHM :L IHM>GM :L H??>K>= ;R
MA> EB@GBG =>@K:=BG@ ?NG@B 89 )B@GBG =>@K:=BG@ ;:<M>KB: I:K
MB<NE:KER BG<EN=> :<MBGHFR<>M>L αIKHM>H;:<M>KB: :G= γIKHM>H;:<M>KB:
89 #HK BGLM:G<> : βIKHM>H;:<M>KBNF LMK:BG ;68/'</*;9 ('9/2+49/9
 <:G NL> EB@GBG :L MA> LHE> <:K;HG LHNK<> :G= BM K>LNEM>= 
EB@GBG K>FHO:E :?M>K  =:RL 89 &G :==BMBHG LHF> :>KH;B< ;:<M>KB: EBD>
.5*5)5));9059:// LI !6./4-5(/;3 LI :G= ')/22;9 LI <:G IKH=N<> EB@GBG
=>@K:=BG@ >GSRF>L 89
,G MA> HMA>K A:G= AR=KHERMB< ;:<M>KB: :K> BFIHKM:GM ?HK MA> IK>
MK>:MF>GM H? FB<KH:E@:> PAB<A IKH=N<> AR=KHERMB< >GSRF>L <HGMBGN
HNLER =NKBG@ MA>BK @KHPMA NG=>K ?:OHK:;E> <HG=BMBHGL &G MABL K>@:K=
AR=KHERMB< ;:<M>KB: :K> >O>G ;>MM>K MA:G NLBG@ >GSRF>L :L MA> E:M>K
<:G EHL> MA>BK :<MBOBMR NIHG MBF> 89 %R=KHERMB< ;:<M>KB: <:G :ELH ;>
NL>= ?HK IK>MK>:MBG@ )  =NKBG@ ;BH@:L IKH=N<MBHG PAB<A F:R BG<EN=>
259:8/*/;3 LI +22;25354'9 LI ')/22;9 LI ".+83535459658' LI :G=
!:8+6:53?)+9 LI 89 1A>K> A:L ;>>G : @KHPBG@ BGM>K>LM BG ;:<M>KB:E
IK>MK>:MF>GM H? ;BHF:LL NG=>K MA> FB<KH:>KH;B< <HG=BMBHGL ?HK BFIKHO
BG@ F>MA:G> IKH=N<MBHG =NKBG@ :G:>KH;B< =B@>LMBHG &G MABL INKIHL> NG
DGHPG AR=KHERMB< ;:<M>KB: <HGLBLM>= H? ;HMA :G:>KH;B< :G= ?:<NEM:MBO>
;:<M>KB: P>K> LMN=B>= NG=>K MA>KFHIABEB< <HG=BMBHG NLBG@ ;BH@:L LENKKR
MA:M K>LNEM>= : LB@GB?B<:GM BFIKHO>F>GM BG F>MA:G> RB>E= 89 %HP
>O>K NMBEBS:MBHG H? MA>KFHIABEB< :G= ?:<NEM:MBO> ;:<M>KB:E FBQMNK>L BG
;BHF:LL IK>MK>:MF>GM <HNE= ;> <A:EE>G@BG@ BG F:BGM:BGBG@ MA> HIMBFNF
@KHPMA <HG=BMBHGL ?HK =B??>K>GM ;:<M>KB:E LI><B>L
%R=KHERMB< ;:<M>KB: NL>= ?HK IK>MK>:MBG@ FB<KH:E@:> F:BGER BGOHEO>L
;:<M>KB:E LI><B>L MA:M A:O> :E@B<B=:E <:I:;BEBMR %HP>O>K MA>K> :K>
HGER : ?>P K>IHKML HG MA> NL:@> H? :E@B<B=:E ;:<M>KB: ?HK IK>MK>:M
F>GM H? FB<KH:E@:> 89 &G :G >:KEB>K LMN=R .258+22' <;2-'8/9
P:L IK>MK>:M>= PBMA ')/22;9 :.;8/4-/+49/9 &1/&$ LMK:BG ?HK EBIB= >Q
MK:<MBHG ?KHF MA> FB<KH:E@: =NKBG@ ;BH=B>L>E IKH=N<MBHG PAB<A K>
LNEM>=  BG<K>:L> BG EBIB= K><HO>KR 89 GHMA>K LMN=R P:L =HG>
?HK =BLKNIMBG@ MA> <>EE P:EE H? .258+22' LI NLBG@ ')/22;9
Table 3
"??><ML H? LHF> L>E><M>= PABM>KHM ?NG@:E IK>MK>:MF>GM HG MA> EB@GH<>EENEHLB< <HGM>GML H? ) 
#NG@:ELMK:BG BHF:LL
NEMNK>
M><AGBJN>
-K>MK>:MF>GM
MBF>=
0>E><MBOBMR
O:EN>
)B@GBG
K>FHO:E
%>FB<>EENEHL>
EHLL
>EENEHL>
EHLL
/>F:KDLHO>K:EE
>k<B>G<R p: />?
2+;85:;959:8+':;9 /B<>LMK:P 0N;F>K@>=      *>=BNF 89
6;2354'8/;3 0N@:K<:G>MHI 0N;F>K@>=      %B@A 89
9;(<+83/9658' HKGLMHO>K 0HEB=LM:M>     %B@A 89
9;(<+83/9658' /4;9:'+*' PHH=<ABIL 0HEB=LM:M>     &G>k<B>GM 89
9;(<+83/9658' *BL<:GMANL 0N;F>K@>=      89
9;(<+83/9658' /N;;>KPHH= 0HEB=LM:M>      $HH= 89
9;(<+83/9658' /4;98'*/':' PHH=
<ABIL
0HEB=LM:M>      )HP 89
9;(<+83/9658' HKGLMHO>K 0HEB=LM:M>      %B@A 89
9;(<+83/9658' 0PBM<A@K:LL 0HEB=LM:M>      %B@A 89
9;(<+83/9658' 4A>:MLMK:P 0HEB=LM:M>   *>=BNF 89
9;(<+83/9658' %:K=PHH=L 0HEB=LM:M>      %B@A 89
'45*+83'
';9:8'2+
/4;98'*/':' PHH=
<ABIL
0HEB=LM:M>      )HP 89
?)45658;9 LI 0PBM<A@K:LL 0HEB=LM:M>  − ∼ − − 89
"8'3+:+9./89;:' HKGLMHO>K 0HEB=LM:M>      *>=BNF 89
+8/658/'2')+8':' ':I:G>L>K>=IBG>
/4;9*+49/B58'
0N;F>K@>=   − − 89
!:+8+;3./89;:;3 ':I:G>L>K>=IBG> 0N;F>K@>=   − − 89
52?658;9(8;3'2/9 ':I:G>L>K>=IBG> 0N;F>K@>=   − − 89
"8'3+:+9<+89/)5258 /N;;>KPHH=+<+'
(8'9/2/+49/9
0HEB=LM:M>      )HP 89
.'4+85).'+:+
).8?959658/;3
HMMHGLM:ED 0HEB=LM:M>   − − 89
).8?959658/;3 HMMHGLM:ED 0N;F>K@>=   − − 89
).8?959658/;3 HKGLMHO>K 0N;F>K@>=      )HP 89
?':.;96'22/*;9 HKGLMHO>K 0N;F>K@>=      )HP 89
?)45658;9
9'4-;/4+;9
HKGLMHO>K 0N;F>K@>=      )HP 89
?':.;99:+8)58+;9 HKGLMHO>K 0N;F>K@>=      )HP 89
52?658;9
)536'):;9
HKGLMHO>K 0N;F>K@>=      )HP 89
.2+(/'(8+</9658' HKGLMHO>K 0N;F>K@>=      *>=BNF 89
: ,O>K:EE I>K?HKF:G<> P:L <HGLB=>K>= ;:L>= HG L>E><MBOBMR O:EN>   BG>k<B>GM   EHP   F>=BNF  @HH=   AB@AER >k<B>GM
UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
2/).+4/,583/9 MA:M >Q>KM>= LB@GB?B<:GM >??><ML PBMABG  A 89 &G :
K><>GM LMN=R GBG> <>EENEHERMB< ;:<M>KB:E LMK:BGL ;>EHG@BG@ MH MA> @>G
>K: +85354'99+;*5354'9.8?9+5('):+8/;3 :G= '5;2:+22' P>K> BLH
E:M>= :G= >O:EN:M>= ?HK FB<KH:E@:E IK>MK>:MF>GM MA:M K>LNEM>=
 AB@A>K F>MA:G> RB>E= =NKBG@ :G:>KH;B< =B@>LMBHG
 /525-/)'2 68+:8+':3+4: (? 3/)85(/'2 )54958:/'
- BL <HFFHGER =HG> ;R >FIEHRBG@ INK> <NEMNK>L H? ?NG@B :G= ;:<
M>KB: %HP>O>K MA>K> :K> MPH F:CHK IKH;E>FL PBMA MA> INK> <NEMNK> LRL
M>FL  K>JNBK>F>GM MH LMKB<MER F:BGM:BG MA> <HG=BMBHGL >LL>GMB:E ?HK
INK> <NEMNK> :L P>EE :L MA> BG:;BEBMR H? <:KKRBG@ HNM IK>MK>:MF>GM BG :G
HI>G LRLM>F :G=  K>JNBK>F>GM H? EHG@ IK>MK>:MF>GM MBF> 89 
G>P BGLB@AM H? =>O>EHIBG@ :G= NLBG@ FB<KH;B:E <HGLHKMB: <HNE= A:O> IH
M>GMB:E ?HK HO>K<HFBG@ MA>L> BLLN>L *B<KH;B:E <HGLHKMB: <HGLBLM H? FBQ
MNK>L H? FB<KHHK@:GBLFL MA:M PHKD LRG>K@BLMB<:EER HG MA> ;BHF:LL =NK
BG@ IK>MK>:MF>GM 89 &G ?:<M FB<KHHK@:GBLFL BG G:MNK> EBO> BG
MA> <HFFNGBMR :G= <:KKR HNM LRG>K@BLMB< F>M:;HEB< :<MBOBMB>L MH =>@K:=>
<HFIE>Q LN;LMK:M>L PAB<A :K> H?M>G =B?i<NEM MH =><HFIHL> BG=BOB=N:EER
89 1A> LRG>K@BLMB< F>M:;HEB< ?>:MNK>L H;L>KO>= BG FB<KH;B:E <HGLHK
MB: P>K> E:K@>ER LMN=B>= BG K><>GM R>:KL MH :LLBFBE:M> MA>L> ?>:MNK>L BGMH
MA> ;BHIKH<>LL M><AGHEH@R 89 1A> >QIEHBM:MBHG H? MABL <HG<>IM BG
- BL GHP:=:RL <HGLB=>K>= :L HG> H? MA> IKHFBLBG@ M><AGHEH@B<:E IKH
@K>LL>L BG ;BHF:LL IK>MK>:MF>GM
&G :==BMBHG MH K>=N<BG@ MA> IK>MK>:MF>GM MBF> FB<KH;B:E <HGLHKMBNF
LRLM>F H??>KL LHF> HMA>K :=O:GM:@>L HO>K LBG@E> FB<KH;B:E IK>MK>:MF>GM
EBD> BG<K>:L>= :=:IM:;BEBMR BFIKHO>= AR=KHERLBL >?i<B>G<R :G= IKH=N<
MBOBMR <HGMKHEE>= I% :G= BG<K>:L>= LN;LMK:M> NMBEBS:MBHG 89 %HP
>O>K =>LIBM> MA> IKHFBLBG@ ?>:MNK>L FB<KH;B:E <HGLHKMB: A:O> LHF>
M><AGB<:E =B?i<NEMB>L ?HK MA> K>:EPHKE= :IIEB<:MBHG LN<A :L LM:;BEBMR H?
FB<KHHK@:GBLFL BG MA> <HGLHKMB: :G= F:BGM>G:G<> H? MA> >??><MBO> <HG
=BMBHGL ?HK LRG>K@BLMB< :<MBOBMB>L H? MA> IHINE:MBHG 1A> >??><MBO> <HGLHK
MBNF LRLM>F BL =>LBK>= MH ;> LM:;E> :G= K>@NE:MBO> PBMA H??>KBG@ MA> >G
A:G<>= AR=KHERMB< >?i<B>G<R 89
*B<KH;B:E <HGLHKMBNF A:L I:KMB<NE:KER ;>>G =K:PG :MM>GMBHG BG MA>
IK>MK>:MF>GM H? ;BHF:LL NL>= BG :G:>KH;B< =B@>LMBHG ?HK ;BH@:L;BH
F>MA:G> IKH=N<MBHG LBG<> MABL LRLM>F <:G LB@GB?B<:GMER BG<K>:L> MA>
;BH=>@K:=:;BEBMR H? MA> ;BHF:LL ;R K>=N<BG@ MA> <KRLM:EEBGBMR :G= =>
@K:=BG@ MA> F:<KHFHE><NE>L 89 *HK>HO>K BG LHF> <:L>L FB<KH
;B:E <HGLHKMB: P>K> :IIEB>= ?HK MA> INKIHL> H? >GSRF:MB< IK>MK>:MF>GM H?
;BHF:LL BG MA> :G:>KH;B< =B@>LM>K NLBG@ HGER <>EENEHERMB< ;:<M>KB: 89
"O>G MAHN@A :IIEB<:MBHG H? FB<KH;B:E <HGLHKMBNF LRLM>F BL IK>=HFBG:GM
BG ;BH@:L IKH=N<MBHG BM A:L :ELH LMN=B>= BG MA> IKH=N<MBHG H? HMA>K ;BH
?N>EL EBD> ;BH>MA:GHE 89 &G MA> I:LM R>:KL F:GR FB<KH;B:E <HGLHK
MB: A:O> ;>>G LMN=B>= ?HK IK>MK>:MF>GM H? ;BHF:LL PBMA MA> HNM<HF>L H?
LB@GB?B<:GM BFIKHO>F>GML BG MA> HO>K:EE ;BH<HGO>KLBHG >?i<B>G<B>L :G=
;BH?N>E RB>E=L PAB<A BG<EN=>= ;:<M>KB: 89 ?NG@:E 89 :G= ?NG
@:E;:<M>KB:E 89 &G :==BMBHG KNF>G FB<KHjHK: <HNE= :ELH A:O> IH
M>GMB:E MH ;> NL>= :L FB<KH;B:E <HGLHKMB: ?HK ;BHF:LL IK>MK>:MF>GM 89
4>G >M :E 89 A:O> LMN=B>= MAK>> <HGLHKMBNF LRLM>FL ?HK IK>
MK>:MBG@ +:IB>K @K:LL =NKBG@ F>MA:G> IKH=N<MBHG MA:M IKH=N<>=
?HE=L AB@A>K :FHNGML H? F>MA:G> <HFI:K>= MH MA> RB>E= H?
NGMK>:M>= @K:LL &G :GHMA>K LMN=R : LRGMA>MB< FB<KH;B:E <HGLHKMBNF P:L
=>O>EHI>= NLBG@ : ;:<M>KB:E :G= : ?NG@:E LI><B>L =NKBG@ IK>MK>:MF>GM
H? <HKG LMHO>KL PAB<A LB@GB?B<:GMER >GA:G<>= MA> RB>E= H? BLH;NM:GHE
89 /><>GMER : GHO>E <HGLHKMBNF LRLM>F <HGLBLMBG@ H?  ?NG@:E 
;:<M>KB:E :G=  :<MBGHFR<>M>L LMK:BGL P:L :IIEB>= BG MA> IK>MK>:MF>GM
H? MK>> MKBFFBG@L MA:M =>@K:=>= EB@GBG BG  = PBMA : AB@A L>E><MBOBMR
O:EN>  89 ":KEB>K : <HFIE>Q <HGLHKMBNF P:L K>IHKM>= =NKBG@
F>MA:G> IKH=N<MBHG ?KHF <HKG LMHO>KL MA:M <HFIHL>= H?  R>:LML  <>E
ENEHERMB< ;:<M>KB:  EB@GBGHERMB< 4/# :G=  E:<MB< :<B= ;:<M>KB: PAB<A
>GA:G<>= F>MA:G> RB>E= ;R  :?M>K  = 89
 /525-/)'2 68+:8+':3+4: (? +49/2/4-
"GLBEBG@ ;:LB<:EER BGOHEO>L :==BMBHG H? FB<KHHK@:GBLFL MH ;BHF:LL
>GLBE:@>L IK>?>K>GMB:EER E:<MB< :<B= ;:<M>KB: 1A> >GLBEBG@ ;:<M>KB: IKH
=N<> =B??>K>GM HK@:GB< :<B=L MAKHN@A LHEB=LM:M> ?>KF>GM:MBHG :G= =>
<K>:L> I% H? MA> LNKKHNG=BG@ >GOBKHGF>GM 1ABL :<B=B< <HG=BMBHG BG
<K>:L>L =B@>LMB;BEBMR H? MA> IHERL:<<A:KB=>L IKHM><ML @KHPMA H? NG
P:GM>= FB<KHHK@:GBLFL :G= IKHOB=>L :G >Q<>EE>GM >GOBKHGF>GM ?HK LB
FNEM:G>HNL P>:D :<B= IK>MK>:MF>GM 89 &G MABL P:R >GLBEBG@ H?
?>KL MA> HIMBHG ?HK LBFNEM:G>HNL LMHK:@> :G= IK>MK>:MF>GM H? ;BHF:LL :M
P>M LM:@>L <:NLBG@ : EHP EHLL H? ?>KF>GM:;E> <HFIHG>GML 89 1A> :I
IEB<:MBHG H? >GLBEBG@ P:L iKLM K>IHKM>= BG  !>P:K >M :E 89 PAH
K>IHKM>= MA:M LMHK:@> MBF> H? KR> @K:LL <HNE= ;> BG<K>:L>= NG=>K >GLBEBG@
<HG=BMBHGL ;R @>G>K:MBG@ :<B=B< <HG=BMBHGL :G= =>@K:=BG@ A>FB<>EENEHL>
MAKHN@A MA> :<MBOBMR H? >GSRF>L L><K>M>= ;R @K:LL BML>E?
1A> >?i<B>G<R H? >GLBEBG@ BL AHP>O>K :??><M>= ;R L>O>K:E BGMKBGLB<
:G= >QMKBGLB< ?:<MHKL 1HM:E LHEB= <HGM>GM BG MA> LBEHL BL HG> H? LN<A ?:<
MHKL MA:M <:G :??><M O:KBHNL :<MBOBMB>L H? >GLBEBG@ I>KM:BGBG@ MH F:LL MK:GL
?>K >?jN>GM KNGHm <HFIHLBMBHG H? >GLBE>= ;BHF:LL :G= M:K@>M IKH=N<M
RB>E=  EHP <HGM>GM H? LHEB= BG LBEHL F:R <:NL>L >?jN>GM KNGHm PAB<A
<:G ;> FBGBFBS>= ;R NLBG@ <H>GLBEBG@ M><AGHEH@R &G MABL M><AGBJN>
AB@A LHEB= <HGM>GM H? MA> F:CHK ;BHF:LL G>:KER  BL >GLBE>= PBMA :G
HMA>K <H>GLBEBG@ ;BHF:LL 89 GHMA>K <HG<>KG PBMA >GLBEBG@ BL MA>
K>JNBK>F>GM H? K>E:MBO>ER EHG@>K MBF> <HFI:K>= MH MA> MBF> G>>=>= >O>G
?HK HMA>K - %HP>O>K EHG@ IK>MK>:MF>GM MBF> =NKBG@ >GLBEBG@ <:G HMA
>KPBL> ;> HO>KEHHD>= :L BM H??>KL <HF;BG>= ?:<BEBMB>L H? LMHK:@> :G= IK>
MK>:MF>GM *HK>HO>K >GLBEBG@ H??>KL IHM>GMB:E :=O:GM:@>L BG =><K>:LBG@
>G>K@R <HGLNFIMBHG ;R IKHOB=BG@ MA> ?:<BEBMR MH LMHK> ;BHF:LL :M P>M
LM:@>L MA:M :OHB=>= >G>K@R K>JNBK>F>GM ?HK =KRBG@ 89 &G :==BMBHG BM
<:G ;> =HG> :M MA> :F;B>GM M>FI>K:MNK> :G= IK>LLNK> MA:M IKHOB=>L HI
IHKMNGBMR ?HK HG?:KF LMHK:@> :G= IK>MK>:MF>GM H? ;BHF:LL 89
"GLBEBG@ K>JNBK>L IK>L>G<> H? P:M>KLHEN;E> LN@:KL MH IKH=N<> K:IB=
:G= AB@A :FHNGML H? HK@:GB< :<B=L 89 :G= FBGBFBS> EHLL H? IHERL:<
<A:KB=>L 89 2G?HKMNG:M>ER FHLM H? MA> ;BHF:LL =H GHM <HGM:BG LN?
i<B>GM :FHNGML H? ?K>> LN@:KL :G= A>G<> G><>LL:KR LN@:K E>O>E LAHNE=
;> F:BGM:BG>= ;R :==BG@ :G >QM>KG:E LN@:K LHNK<> HK FH=B?RBG@ MA>
IKH<>LL G >Q:FIE> H? IKH<>LL FH=Bi<:MBHG BL MA> :==BMBHG H? <>EENE:L>L
:G= A>FB<>EENE:L>L MH MA> >GLBE:@>= ;BHF:LL MH =>@K:=> AHEEH<>EENEHL>
BGMH LHEN;E> LN@:KL =NKBG@ >GLBEBG@ 89 %HP>O>K NMBEBS:MBHG H? >QM>K
G:E >GSRF>L F:R GHM ;> M><AGH><HGHFB<:EER K>:EBLMB< =N> MH MA> <HLML
H? >GSRF>L EHLL H? IHERL:<<A:KB=>L =NKBG@ >GLBEBG@ :G= K>JNBK>F>GML
?HK F:BGM:BGBG@ =B??>K>GM <HG=BMBHGL ?HK >GSRF> :<MBOBMB>L :G= @KHPMA
H? >GLBE:@>= ;:<M>KB:E 0N@:K E>O>E <:G :EM>KG:MBO>ER ;> F:BGM:BG>= ;R
:==BG@ : LHNK<> H? ?K>> LN@:KL MH MA> >GLBE:@>= ;BHF:LL LN<A :L FH
E:LL>L 89 "O>G MAHN@A :==BMBHG H? ?K>> LN@:KL H??>KL :=O:GM:@>L HO>K
MA> NMBEBS:MBHG H? >QM>KG:E >GSRF> LRLM>FL BM ?NKMA>K BG<K>:L>L HO>K:EE
IKH<>LL <HLML :G= F:R F:D> MA> IKH<>LL <HFIE>Q
&? MA> :;HO> ?:<ML :K> M:D>G BGMH <HGLB=>K:MBHG >GLBEBG@ F:R GHM
;> : LM:G=:EHG> IK>MK>:MF>GM F>MAH= ?HK FHLM H? MA> :O:BE:;E> ;BH
F:LL LN<A :L <>K>:E LMK:PL GHKF:E <HKG LMHO>KL LH?MPHH=L :G= A:K=
PHH=L 1A> >GLBEBG@ <HNE= ;> FHK> >??><MBO> ?HK IK>MK>:MBG@ MA> ;BH
F:LL MA:M <HGM:BG BG=B@>GHNL ?K>> LN@:KL LN<A :L LP>>M <HKG LMHO>KL
89 LP>>M LHK@ANF LM:EDL 89 :G= LN@:K ;>>M E>:O>L 89 ,G
MA> HMA>K A:G= <H>GLBEBG@ H? GHKF:E ;BHF:LL PBMA MA> LN@:K <HGM:BG
BG@ ;BHF:LL F:R >QM>G= MA> :IIEB<:MBHG H? >GLBEBG@ MH MA> ;BHF:LL PBMA
GH HK IHHK LN@:KL 1ABL M><AGBJN> :ELH >Q>KML IHLBMBO> >??><ML HG MA> ;BH
F:LL :M K>E:MBO>ER LAHKM>K K>M>GMBHG MBF> #HK >Q:FIE> : K><>GM LMN=R
K>IHKM>= MA:M <H>GLBEBG@ H? PA>:M LMK:PL :G= LN@:K ;>>M E>:O>L K>
LNEM>= IHLBMBO> >??><ML HG ;BH<A>FB<:E F>MA:GHE IHM>GMB:E *- :?M>K

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
 =:RL PABE> GHKF:E >GLBEBG@ M:D>L FHK> MA:G  =:RL ?HK @>MMBG@ LN<A
IHLBMBO> >??><ML HG *- ?KHF LMK:PL 89
&G : <H>GLBEBG@ LRLM>F LN@:K <HGM:BGBG@ ;BHF:LL IKHOB=>L LHF>
;>G>iML MH MA> HO>K:EE >GLBEBG@ IKH<>LL &M =>EBO>KL FHBLMNK> :G= LN@:KL
MH <:KKR HNM ?>KF>GM:MBHG :G= AR=KHERLBL H? GHGLN@:KR ;BHF:LL +>O
>KMA>E>LL >GLBEBG@ A:L EBFBM>= >??><M HG EB@GBG K>FHO:E 89 &G ?:<M
MA> HO>K:EE IK>MK>:MF>GM <HG=BMBHGL BG :G >GLBEBG@ LRLM>F L>>FL MH ;>
GHM <HFI>MBMBO> PBMA MA> HMA>K IK>MK>:MF>GM :IIKH:<A>L ?HK E:K@> L<:E>
?:<BEBMB>L %HP>O>K ?NKMA>K >??HKML <:G ;> F:=> MH F:D> >GLBEBG@ :L
: LM:G=:EHG> IK>MK>:MF>GM F>MAH= ;R :IIERBG@ LMKN<MNK> LI><Bi< >G
SRF>L BG >GLBE:@>L :EHG@ PBMA MA> =>O>EHIF>GM H? :IIKHIKB:M> >GSRF>L
FBQMNK>L BG :==BMBHG MH >LMBF:MBG@ MA> ?:M> H? <>EE P:EE F:M>KB:EL BG :
MRIB<:E >GLBEBG@ LRLM>F 89
 /525-/)'2 68+:8+':3+4: (? +4@?3+9
"GSRF:MB< IK>MK>:MF>GM BGOHEO>L NMBEBS:MBHG H? <KN=> HK INKBi>= HK
L>FBINKBi>= >GSRF>L =>KBO>= ?KHF MA> @KHNIL >BMA>K EB@GBGHERMB< HK AR
=KHERMB< >GSRF>L "GSRF:MB< IK>MK>:MF>GM H? )  NLBG@ EB@GBGHERMB< >G
SRF>L F:R K>LNEML BG MA> K>FHO:E H? :EFHLM LBFBE:K :FHNGML H? EB@GBG
:L H;M:BG>= ?KHF MA> ?NG@:E IK>MK>:MF>GM !>LIBM> MA> G>>= H? K>E:
MBO>ER LAHKM>K MBF> BG >GSRF:MB< IK>MK>:MF>GM 89 ?>P K>IHKML :K>
:O:BE:;E> HG MA> >GSRF:MB< IK>MK>:MF>GM H? )  NLBG@ EB@GBGHERMB< >G
SRF>L *HK>HO>K MA> HO>K:EE >?i<B>G<R H? >GSRF:MB< =>EB@GBi<:MBHG BL
R>M MH ;> M><AGH><HGHFB<:EER <HFI>MBMBO> MH MA> MK:=BMBHG:E MA>KFH
<A>FB<:E F>MAH=L #HK >Q:FIE> LN@:K<:G> ;:@:LL> P:L IK>MK>:M>= L>I
:K:M>ER PBMA :ED:EBG> +:,% :G= EB@GBGHERMB< >GSRF>L <KN=> >GSRF>
>QMK:<ML ?KHF : 4/# =NKBG@ ;BH>MA:GHE IKH=N<MBHG MA:M K>LNEM>= BG MA>
K>FHO:E H?  :G=  EB@GBG ;R +:,% >GSRF:MB< F>MAH=L K>
LI><MBO>ER /+  EHP>K =>EB@GBi<:MBHG ;R >GSRF:MB< F>MAH= 89
&GM>K>LMBG@ER MA> =HPGLMK>:F <>EENEHL> AR=KHERLBL RB>E= P:L AB@A>K BG
MA> >GSRF:MB< IK>MK>:M>= ;:@:LL>  MA:G MA> RB>E= H;M:BG>= ?KHF
MA> :ED:EB IK>MK>:M>= ;BHF:LL  89 1A> K>:LHGL ?HK LN<A iG=
BG@L <HNE= ;> :MMKB;NM>= MH MA> IK>L>G<> H? ;HMA EB@GBGHERMB< :G= <>EEN
EHERMB< >GSRF>L BG MA> <KN=> >GSRF> >QMK:<ML MA:M :EM>K>= MA> LMKN<MNK>L
H? <>EENEHL> i;>KL =NKBG@ IK>MK>:MF>GM :G= F:=> BM FHK> ?:OHK:;E> ?HK
LN;L>JN>GM AR=KHERLBL 89
IIEB<:MBHG H? AR=KHERMB< >GSRF>L BG MA> IK>MK>:MF>GM H? FB<KH:E
@:> <HNE= ;> IKHFBLBG@ ?HK MA> L>E><MBO> AR=KHERLBL H? MA> ;BHIHER
F>KL :G= =><K>:LBG@ MA> K><:E<BMK:G<> H? <>EE P:EEL 1BEE GHP >GSRF:MB<
IK>MK>:MF>GM H? FB<KH:E@:> A:L ;>>G LMN=B>= ?HK O:KBHNL INKIHL>L BG
<EN=BG@ ;BH@:L F>MA:G> IKH=N<MBHG 89 ;BHAR=KH@>G IKH=N<MBHG
89 EBIB= >QMK:<MBHG ?HK ;BH=B>L>E 89 ;BH>MA:GHE IKH=N<
MBHG 89 :G= IKHM>BG LHEN;BEBS:MBHG 89 &G :==BMBHG MH MA>
MK:=BMBHG:E AR=KHERMB< >GSRF>L *G- BG MA> <KN=> >QMK:<M H? : 4/#
4:.8')56.?22;3 */9)5258 P:L :ELH <HGLB=>K>= =NKBG@ IK>MK>:MF>GM H?
5:8?5)5));9 (8';4// ?HK ;BH@:L IKH=N<MBHG 89 "GSRF:MB< IK>MK>:M
F>GM <:G ;> =HG> ;R NMBEBS:MBHG H? >BMA>K LBG@E> >GSRF> HK >GSRF> <H<D
M:BEL "GSRF> <H<DM:BEL :K> MA> FBQMNK>L H? INK> >GSRF>L HK <KN=> >Q
MK:<ML H? ;:<M>KB: :G= ?NG@B <HGM:BGBG@ FBQMNK>L H? >GSRF>L EMAHN@A
LBG@E> >GSRF> LRLM>FL P>K> K>IHKM>= ?HK AB@A>K =HPGLMK>:F RB>E= <HF
I:K>= MH NGMK>:M>= FB<KH:E@:> ;BHF:LL 89 >GSRF> <H<DM:BEL
PHNE= ;> FHK> IKHFBLBG@ ?HK AR=KHERSBG@ =B??>K>GM ;BHIHERF>KL H? MA>
;BHF:LL 89
"O>G MAHN@A <HFF>K<B:E >GSRF> LRLM>FL P>K> :IIEB>= BG >:KEB>K
LMN=B>L 89 <KN=> >GSRF> <H<DM:BEL <HNE= ;> FHK> IKHFBLBG@
?HK >GSRF:MB< IK>MK>:MF>GM BG M>KF H? M><AGH><HGHFB< BLLN>L KN=>
>GSRF>L <:G ;> IKH=N<>= :G= <HEE><M>= >:LBER ;R ?NG@B :G= ;:<M>KB:
<:I:;E> H? L><K>MBG@ >QMK:<>EENE:K >GSRF>L 89 &G : K><>GM LMN=R
<KN=> >GSRF>L P>K> IKH=N<>= ;R 96+8-/22;9 2+4:;2;9 :G= :IIEB>= ?HK
IK>MK>:MF>GM H? FB<KH:E@:E ;BHF:LL PAB<A A:L K>LNEM>= LB@GB?B<:GM BF
IKHO>F>GM BG ,! LHEN;BEBS:MBHG :M : =HL> H?  OO
:?M>K  A :M ` 89 &G :==BMBHG MA> IK>MK>:MF>GM MBF> P:L ?NKMA>K
K>=N<>= MH HGER  FBG ?HK MA> L:F> >GSRF:MB< LRLM>F :G= ;BHF:LL ;R
<HG=N<MBG@ MA> IK>MK>:MF>GM NG=>K MA> HIMBFNF I% :G= M>FI>K:MNK> 
:G=  `  K>LI><MBO>ER 89
4. Mechanisms of biological pretreatment
 +2/-4/A)':/54 5,  (? 9+)8+:/4- 2/-4/452?:/) +4@?3+9
1A> F:CHK F><A:GBLF H? EB@GBG ;K>:D=HPG ;R 4/# :G= ;:<M>KB: BL
MA> IKH=N<MBHG H? >QMK:<>EENE:K >GSRF>L NG=>K GNMKB>GM =>IE>MBHG <HG=B
MBHGL MA:M <:M:ERS> O:KBHNL ;BH<A>FB<:E K>:<MBHGL ?HK EB@GBG ;K>:D=HPG
FHG@ MA> EB@GBG=>@K:=BG@ >GSRF>L MA> FHLM <HFFHG :K> E:<<:L>
;>GS>G>=BHE HQR@>G HQB=HK>=N<M:L>L "  EB@GBG I>KHQB=:L>
)B- "  :G= F:G@:G>L> I>KHQB=:L> *G- " 
1A> MAK>> EB@GBGHERMB< >GSRF>L =B??>K ?KHF >:<A HMA>K PBMA K>@:K= MH
LHNK<> IKHI>KMB>L :G= FH=> H? :<MBHG BG EB@GBG K>FHO:E 1:;E>  
?>P HMA>K E>LL <HFFHG EB@GBG=>@K:=BG@ >GSRF>L P>K> :ELH K>IHKM>=
?KHF >BMA>K ?NG@B HK ;:<M>KB: #HK >Q:FIE> O>KL:MBE> I>KHQB=:L> 3-
"  :KRE:E<HAHE HQB=:L> , "  @ERHQ:E HQB=:L>
$),5 "  :KRE:E<HAHE =>AR=KH@>G:L>L ! " 
IHERIA>GHE HQB=:L> --, "  =R>=><HEHKBSBG@ I>KHQB=:L>
!R- "  :G= JNBGHG> K>=N<M:L>L ./ "  #HK =>
M:BE>= K>OB>P H? MA>L> >GSRF>L K>?>K MH 89
*HLM H? MA> E:<<:L>L LMN=B>= BG MA> I>KLI><MBO> H? ;BHEH@B<:E =>EB@
GBi<:MBHG :K> FHLMER =>KBO>= ?KHF 4/# +HP:=:RL ;:<M>KB:E E:<<:L>L
A:O> :ELH ;>>G LAHPG BGM>K>LM ?HK EB@GBG ;K>:D=HPG :G= :IIEB<:MBHG BG
HMA>K ;BHM><AGHEH@B<:E IKH<>LL>L ):<<:L>L :K> LMKN<MNK:EER FNEMB<HII>K
 N >GSRF>L <HGM:BGBG@ ?HNK N :MHFL BG MA> <:M:ERMB< <>GM>K MA:M IE:R
KHE>L BG MA> <:M:ERMB< :<MBHG 89 EMAHN@A E:<<:L> BL : P>EEDGHPG
EB@GBGHERMB< >GSRF> BM BL : E:K@> FHE><NE> A:OBG@ MA> FHE><NE:K P>B@AM
G>:KER  D!: MA:M <:NL>L =B?i<NEMB>L MH >GM>K =>>I BGMH MA> LN;LMK:M>
:G= A>G<> <:G PHKD HGER HG MA> LNK?:<> H? MA> LN;LMK:M>L 89
&G :==BMBHG E:<<:L>L A:O> EHP K>=HQ IHM>GMB:E  3 PAB<A =H>L
GHM ?:OHK MH HQB=BS> GHGIA>GHEB< <HFIHNG=L NG=>K G:MNK:E <HG=BMBHGL
89 1A> HQB=:MBHG H? MA> IA>GHEB< <HFIHG>GML -  ;R E:<<:L>L H<
<NKL MAKHN@A MA> K:=B<:E <:M:ERS>= F><A:GBLF :L BEENLMK:M>= BG #B@
: %HP>O>K E:<<:L> <:G :<M HG MA> GHGIA>GHEB< <HFIHG>GML +- 
:G= =>>I BGMH MA> LN;LMK:M> BG MA> IK>L>G<> H? LHF> HQB=:MBHG F>=B
:MHKL <:EE>= E:<<:L> F>=B:MHK LRLM>F )*0 89 1A> <HFFHG )*0
BG<EN=> b:SBGH;BL>MARE;>GSMAB:SHEBG>LNE?HG:M> 10 AR
=KHQR;>GSHMKBSHE> %1 +AR=KHQRIAMA:EBFB=> %-& :G= +AR=KHQ
R:<>M:GBEB=> +% 89 1A>L> F>=B:MHKL ;:LB<:EER :<M :L O>AB<E>L
MH MK:GL?>K >E><MKHG =NKBG@ E:<<:L> F>=B:M>= HQB=:MBHG H? GHGIA>GHEB<
<HFIHG>GML #B@ ; &G : MRIB<:E F>=B:MHKF>=B:M>= <:M:ERLBL E:<<:L>
BGBMB:EER HQB=BS>L MA> F>=B:MHK 1A> HQB=BS>= F>=B:MHK MA>G FHO>L MH MA>
<>EE P:EE PA>K> BM BL K>=N<>= MH BML BGBMB:E ?HKF ;R MA> LN;LMK:M> #B@ <
)B- :ELH <:EE>= EB@GBG:L> BL : A>F><HGM:BGBG@ @ER<HIKHM>BG :G=
BL <HFI:K:MBO>ER GHGLI><Bi< MH BML LN;LMK:M>L 89 &M <:G HQB=BS>
;HMA - :G= +- BG MA> IK>L>G<> H? %p,p 89 PA>K> HQB=:MBHG
H? - BL H? IK>?>K>G<> :G= FN<A ?:LM>K <HFI:K>= MH MA> HQB=:MBHG
K:M> H? +- 89 1A> )B-F>=B:M>= HQB=:MBHG H? - :G= +- BGBMB
:M>L PBMA MA> ?HKF:MBHG H? )B-BGM>KF>=B:M> <HFIHNG= & :G= && )B-&
:G= )B-&& PAB<A :K> IKH=N<>= ;R MPH @>G>K:E LM>IL #BKLMER )B-& BL
?HKF>= MAKHN@A : >pHQB=:MBHG H? MA> G:MBO> #>p BG MA> IK>L>G<>
H? %p,p PABE> ?HKF:MBHG H? )B-&& ?HEEHP>= MPH L>JN>GMB:E K>=N<MBHG
H? )B-& PBMA >p>:<A MBF> ?KHF MA> =HGHK LN;LMK:M> #B@  89
&G MA> LN;L>JN>GM :<MBHG H? )B- - L :K> <HGO>KM>= BGMH IA>GHQR K:=
B<:EL MAKHN@A MA> K>=N<MBHG H? )B-& :G= )B-&& PAB<A IA>GHQR K:=B
<:EL MA>K>:?M>K K>:<M BG MA> IK>L>G<> H? HQR@>G :G= @>G>K:M> KBG@<E>:O
:@> IKH=N<ML ,QB=:MBO> K>:<MBHG H? +- H<<NKL ;R MA> @>G>K:MBHG H?
K:=B<:E <:MBHG MAKHN@A >pHQB=:MBHG IKH<>LL ?HEEHP>= ;R LB=><A:BG

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
Table 4
0HNK<>L <A:K:<M>KBLMB<L :G= F><A:GBLF H? MA> F:CHK EB@GBGHERMB< >GSRF>L
+:F>H?
>GSRF>L 0HNK<>L *:CHK<A:K:<M>KBLMB<L *:CHKFH=>H?:<MBHG />F:KDL />?
):<<:L>L %B@A>KIE:GMLBGL><ML?NG@B+- "8'3+:+9
<+89/)5258;:<M>KB:+- @596/8/22;3
2/65,+8;3')/22;92/).+4/,583/9:G=
:<MBGHFR<>M>L+- !:8+6:53?)+9-8/9+;9
*:BGER FHGHF>KB<
@ER<HIKHM>BG K:K>ER
AHFH =BF>KB< A>M>KH
=BF>KB< HK AHFH
M>MK:F>KB<
HGM:BGL FNEMB<HII>K
?HNK :MHFL ;HNG= MH
=Bm>K>GM ;BG=BG@ LBM>L
BGMA>:<MBO>LBM>
>EHG@L MH MA> @KHNI
H?;EN>HQB=:L>L
:M:ERS>L MA> HQB=:MBHG H? IA>GHEB< <HFIH
G>GML -  BG EB@GBG ;R K>=N<BG@ ,p BGMH
%p,
+HGIA>GHEB< <HFIHNG=L +-  <:G :ELH
;> HQB=BS>= BG MA> IK>L>G<> H? LHF> F>=B:
MHKL
- <:G;>
=>@K:=>=
PBMAHNM)*0
;NM+- G>>=L
)*0
89
)B@GBG
I>KHQB=:L>
)B-
#NG@B+- ).8?959658/;3:G=;:<M>KB:
+- ')/22;99;(:/22/9"QBLML BG O:KBHNL BLH
?HKF PAB<A :K> @ER<H
IKHM>BGL PBMA FHE><N
E:KP>B@AM  D!:
HGM:BG FHE><NE> H?
A>F> :L BKHG IKHMH
IHKARKBG &5 BG >:<A
FHE><NE>H?IKHM>BG
2GBJN> ?HK BML AB@A K>
=HQIHM>GMB:E
4HKDL :M NGNLN:EER
EHPI% 
:G HQB=BS> ;HMA -
:G=+-
)B- -KH=N<>L BGM>KF>=B:M> <HFIHNG= &
)B-& MAKHN@A >pHQB=:MBHG H? MA> G:
MBO> #>p BG MA> IK>L>G<> H? %p,p PAB<A
<E>:O>L,,;HG=
/>=N<MBHG H? )B-& ;R MPH L>JN>GMB:E K>=N<
MBHG PBMA >p>:<A MBF> ?KHF MA> =HGHK
LN;LMK:M> K>LNEML BG MA> ?HKF:MBHG H? MA>
<HFIHNG=&&)B-&&
)B-& :G= )B-&& M:D> I:KM BG MA> =>@K:=:MBHG
H?- :G=+- 
#BKLMEB@GBG
=>@K:=BG@
>GSRF>BLHE:M>=
?KHF 
).8?959658/;3
89
*:G@:G>L>
I>KHQB=:L>
*G-
#NG@B+- 2+;85:;9 LI:G=;:<M>KB:+-
;68/'</*;9('9/2+49/9 G :<B=B< :G= @ER<HLR
E:M>= @ER<HIKHM>BG I&

HGM:BG FHE><NE> H?
A>F> :L BKHG IKHMH
IHKARKBG &5 BG >:<A
FHE><NE>H?IKHM>BG
*HE><NE:K P>B@AM
K:G@>L ?KHF  MH
 D!:
*:QBFNF :<MBOBMR <:G
;> ?HNG= :M MA> *Gp
<HG<>GMK:MBHG :;HO>
 μ*
,QB=:MBHG H? *Gp MH *Gp BG MA> IK>L
>G<> H? %p,p PAB<A *Gp BG MNKG ;>BG@
<A>E:M>= PBMA HK@:GB< :<B= <:G :<ML :L :
=BmNLB;E> HQB=:GM :G= HQB=BS>L - BG EB@GBG
1A> *Gp F>=B:M>= HQB=:MBHG H? +- H<
<NKL ;R ?HKFBG@ K>:<MBO> K:=B<:EL K>JNBKBG@
:G:==BMBHG:EF>=B:MHK
:GHQB=BS>
;HMA- :G=
+-
89
)*0 E:<<:L> F>=B:MHK LRLM>F +-  GHGIA>GHEB< <HFIHNG=L -  IA>GHEB< <HFIHNG=L
Fig. 6. :M:ERMB< F><A:GBLF H? E:<<:L> BG EB@GBG =>@K:=:MBHG : ,QB=:MBHG H? IA>GHEB< <HFIHG>GML BG EB@GBG ;R E:<<:L> PBMAHNM K>JNBKBG@ :GR F>=B:MHKL ; HQB=:MBHG H? GHGIA>GHEB<
<HFIHG>GML BG EB@GBG ;R F>=B:MHK 10F>=B:M>= E:<<:L> :G= < />=HQ <R<E> H? : MRIB<:E E:<<:L>F>=B:MHK HQB=:MBHG LRLM>F />=K:PG PBMA FH=Bi<:MBHG ?KHF 89

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
Fig. 7. :M:ERMB< <R<E> H? )B- LAHPBG@ MA> F><A:GBLF H? ?HKFBG@ )B-& :G= )B-&& BGM>KF>
=B:M>L />=K:PG ?KHF 89
<E>:O:@> =>F>MARE:MBHG BGMK:FHE><NE:K :==BMBHG :G= K>:KK:G@>F>GML
BG +- L ?HK =>M:BEL K>?>K MH 89
*G-L :ELH DGHPG :L %p,p =>I>G=>GM HQB=HK>=N<M:L>L :K> >QMK:
<>EENE:K @ER<HIKHM>BGL :G= L><K>M>= BG FNEMBIE> BLH?HKFL <HGM:BGBG@ HG>
FHE><NE> H? A>F> :L BKHG IKHMHIHKIARKBG &5 L@A>K >M :E 
*G- :<ML HG EB@GBG ;R HQB=BSBG@ *Gp MH *Gp BG MA> IK>L>G<> H?
%p,p MRIB<:E K>:<MBHG BL *Gp  %p  %p,p  *Gp  %p,
PAB<A BL MA>G <A>E:M>= MH :G HK@:GB< :<B= EBD> HQ:EB< :G= F:EHGB< :<B=L
1A> <A>E:M>= *Gp <HFIE>Q :<ML :L : LMKHG@ HQB=:GM :G= <:G =B??NL>
MAKHN@AHNM MA> EB@GBG F:MKBQ 89 1A> *Gp <A>E:MHK <:G HQB
=BS> O:KBHNL - L BG EB@GBG MAKHN@A K>:KK:G@>F>GML ;HG= <E>:O:@>L :G=
GHG>GSRF:MB< =>@K:=:MBHG :L LAHPG BG #B@ : 89 %HP>O>K MA>
*Gp <A>E:MHK BL GHM : LMKHG@ HQB=:GM :G= MANL MA> :<MBOBMR H? MABL <HF
IE>Q BL EBFBM>= HGER MH - L 89 ,G MA> HMA>K A:G= *G- <:G HQB=BS>
+- L MAKHN@A MA> ?HKF:MBHG H? K>:<MBO> K:=B<:EL BG MA> IK>L>G<> H? :G
:==BMBHG:E F>=B:MHK FHLMER MABHEL LN<A :L @ENM:MABHG> 89 1A> *Gp
HQB=BS>L MABHEL BGMH MABRE K:=B<:EL PAB<A BG MNKG :<<>IM AR=KH@>G ?KHF
+- :G= ?HKF ;>GSREB< K:=B<:E >GSREB< K:=B<:E LN;L>JN>GMER IKH=N<>L
iG:E IKH=N<ML MAKHN@A GHG>GSRF:MB< K>:<MBHG 4ABE> >GSRF> K>:<MBHG
H? *Gp :ELH <HNIE>L PBMA MA> I>KHQB=:MBHG H? EBIB=L MH <:M:ERS> αβ
:G= β:KRE >MA>K <E>:O:@>L H? MA> FH=>E +- :G= β, EB@GBG K>LI><
MBO>ER #B@ ; 89
Fig. 8. *G- <:M:ERS>= HQB=:MBHG H? IA>GHEB< : :G= GHGIA>GHEB< ; EB@GBG FH=>E <HFIHNG=L />=K:PG PBMA FH=Bi<:MBHG ?KHF 89

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
 +2/-4/A)':/54 5,  :.85;-. +4:549 ).+3/9:8?
1A> /# <:G I:KMB:EER =>@K:=> EB@GBG GHG>GSRF:MB<:EER </' MA> IKBG
<BIE>L H? #>GMHGL <A>FBLMKR PAB<A <:G ;> <HGLB=>K>= :L MA> /4 </<5
<A>FB<:E IK>MK>:MF>GM H? ;BHF:LL 0HF> 4/# EBD>  ).8?959658/;3 <:G
:ELH NL> #>GMHGL <A>FBLMKR ?HK MA> L:F> INKIHL> BG :==BMBHG MH MA>BK
>GSRF:MB< EB@GBG =>@K:=:MBHG LRLM>F 89 %HP>O>K MABL GHG>G
SRF:MB< EB@GBG ;K>:D=HPG BL GHM IK>?>K>GMB:E L : K>LNEM EB@GBG =>@K:
=:MBHG ;R #>GMHGL <A>FBLMKR BL FHLMER IKHGHNG<>= ?HK /#
/# A:L MA> :;BEBMR MH IKH=N<> >QMK:<>EENE:K %p,p MA:M M:D>L I:KM BG
MA> #>GMHGL K>:<MBHG PBMA */O:E:G<>= F>M:E BHGL FHLMER #>p MH IKH
=N<> ?K>> K:=B<:EL :<<HK=BG@ MH MA> "J  :G= "J  PAB<A BG MNKG
HQB=BS> EB@GBG FHE><NE>L 89 1A> AR=KHQRE ?K>> K:=B<:E IKH=N<>= BG
#>GMHGL K>:<MBHG <:G :ELH :MM:<D HG MA> @ERHLB=B< EBGD:@>L BG MA> AHEEH
<>EENEHL> :G= MANL <:G =>@K:=> <>EENEHL> :G= A>FB<>EENEHL> BG :==BMBHG
MH EB@GBG 89 1ABL A:L F:=> EB@GBG =>@K:=:MBHG ;R #>GMHGL <A>F
BLMKR :L MA> GHGL>E><MBO> :IIKH:<A :G= BL E>LL :MMK:<MBO> ?HK ;BHF:LL IK>
MK>:MF>GM 89 1ABL ;HMME>G><D A:L :ELH EBFBM>= MA> NL:@> H? /# ?HK
- H? ;BHF:LL >O>G MAHN@A #>GMHGL <A>FBLMKR A:L MA> IHM>GMB:E MH H??>K
: LMK:B@AM ?HKP:K= :G= >?i<B>GM IK>MK>:MF>GM :IIKH:<A ?HK ;BH?N>E IKH
=N<MBHG 89


 /98;6:/54 5, 3/)85'2-'2 )+229 '4* .?*852?9/9 5, (/5652?3+89 (?
9+)8+:/4- +4@?3+9
1A> AR=KHERMB< FB<KHHK@:GBLFL NL>= ?HK FB<KH:E@:E IK>MK>:MF>GM
@>G>K:EER >Q>KM MA>BK >??><ML HG MA> <>EE P:EE =BLKNIMBHG :G= AR=KHERLBL
H? BGMK:<>EENE:K ;BHIHERF>KL ;R L><K>MBG@ MA> K>LI><MBO> AR=KHERMB< >G
SRF>L !NKBG@ IK>MK>:MF>GM PBMA BG=BOB=N:E FB<KHHK@:GBLF >BMA>K LBG
@E> 89 HK FBQMNK>L H? AR=KHERMB< >GSRF>L F:R ;> L><K>M>= 89
,G MA> HMA>K A:G= IK>MK>:MF>GM PBMA FB<KH;B:E <HGLHKMB: K>LNEML BG
MA> :O:BE:;BEBMR H? <H<DM:BEL H? AR=KHERMB< >GSRF>L 89 !>I>G=BG@
HG MA> FB<KHHK@:GBLFL AR=KHERMB< >GSRF>L F:R BG<EN=> <>EENE:L>L QR
E:G:L>L IKHM>:L>L I><MBG:L>L <ABMBG:L>L ERLHSRF>L EBI:L>L :G= :FR
E:L>L 89 #HK >Q:FIE> FB<KH:E@:E <>EEL P>K> =BLKNIM>= ;R 58
*/' '2-/)/*' MAKHN@A L><K>MBHG H? >QMK:<>EENE:K IKHM>:L>L :L P>EE :L ;K>:D
=HPG H? IKHM>BG 89 )BD>PBL> =BLBGM>@K:MBHG H? FB<KH:E@:E <>EE P:EE
P:L K>IHKM>= ;R 259:8/*/;3 :.+835)+22;3 =N> MH MA> IKH=N<MBHG H? <>EEN
E:L> ;R MA> ;:<M>KBNF 89
FHG@ MA> O:KBHNL ;BHIHERF>KL IK>L>GM BG FB<KH:E@:E <>EEL BFIHK
M:GM <HFIHG>GML ?HK ;BH?N>EL IKH=N<MBHG :K> IHERL:<<A:KB=>L <>EENEHL>
:G= LM:K<A EBIB= :G= IKHM>BG 1A> AR=KHERLBL I:MM>KG H? MA> F:CHK
;BHIHERF>KL MAKHN@A MA> :<MBHG H? K>LI><MBO> AR=KHERMB< >GSRF>L :K>
LAHPG BG #B@  EMAHN@A AR=KHERLBL H? >:<A <HFIHG>GM BL LAHPG NG
MBE MA> ?HKF:MBHG H? K>LI><MBO> iG:E IKH=N<M IKH@K>LL BG AR=KHERLBL :L
P>EE :L MA> ?HKF:MBHG H? iG:E IKH=N<M LHE>ER =>I>G= HG MA> >?i<B>G<R
H? MA> LMK:BG MRI>L :G= E>O>E H? L><K>M>= >GSRF>L :G= IKH<>LL <HG=B
MBHG :L>= HG MA>L> ?:<MHKL AR=KHERLBL H? MA> ;BHFHE><NE>L F:R ;> >B
MA>K <HFIE>M> HK BG<HFIE>M> &G @>G>K:E MA> <HFIE>M> ;K>:D=HPG H? <>E
ENEHL> H? FB<KH:E@:E ;BHF:LL H<<NKL ;R MA> LRG>K@BLMB< :<MBHG H? MAK>>
<>EENEHERMB< >GSRF>L #B@ : #BKLMER :G >G=Hβ @EN<:G:L> K:G
=HFER <E>:O>L MA> BGM>KG:E β@ER<HLB=B< EBGD:@>L H? <>EENEHL> <A:BG 0><
HG=ER :G >QHβ @EN<:G:L> ;K>:DL β@ER<HLB=B< EBGD:@>L ?KHF MA>
>G= H? <>EENEHL> FHE><NE> :G= @>G>K:M> LF:EE>K HEB@HL:<<A:KB=>L PAB<A
:K> ?NKMA>K ;KHD>G =HPG BGMH @EN<HL> :G= F:EMHL> ;R MA> :<MBHG H? :
β@EN<HLB=:L> 89
0M:K<A BL :GHMA>K FHLM BFIHKM:GM IHERL:<<A:KB=>L :G= BL IK>L>GM BG
LB=> MA> FB<KH:E@:E <>EEL ?M>K =BLKNIMBHG H? <>EEL LM:K<A BL :O:BE:;E> ?HK
:FREHERMB< <HGO>KLBHG %HP>O>K MA> IKH<>LL BL EBMME> ;BM <HFIE>Q :G=
G>>=L MA> :<MBHGL H? LHF> >GSRF>L :L LAHPG BG #B@ ; #BKLMER MPH
@KHNIL H? :FREHERMB< >GSRF>L BLH:FRE:L>L :G= INEENE:G:L>L :<M HG MA>
:FREHI><MBG H? LM:K<A :G= <HGO>KM BM BGMH :FREHL> ;R <E>:OBG@ α
@ER<HLB=B< EBGD:@> 0N;L>JN>GMER >G=Hα:FRE:L>L PHKD K:G=HFER HG
:FREHL> :G= K>F:BGBG@ :FREHI><MBG PAB<A K>LNEML MA> ;K>:D=HPG H?
;HMA IHERL:<<A:KB=> BGMH @EN<HL> F:EMHL> :G= F:EMH=>QMKBGL )BD>PBL>
>QHβ–:FRE:L>L :<M HG MA> GHGK>=N<BG@ >G=L H? :FREHL> :G= <HGO>KM>=
BM BGMH F:EMHL> #BG:EER F:EMHL> BL ;KHD>G =HPG BGMH @EN<HL> ;R MA> :<
MBHG H? @EN<H:FRE:L>L 89 -KHM>BG BG MA> FB<KH:E@:> ;BHF:LL BL AR
=KHERS>= ;R MA> :<MBHG H? IKHM>HERMB< >GSRF>L &GBMB:EER MA> IHERI>I
MB=> <A:BG H? IKHM>BG BL ;KHD>G =HPG BGMH LF:EE I>IMB=>L MA:M :K> LN;
L>JN>GMER <HGO>KM>= BGMH MA> :FBGH :<B=L #B@ < )BIB= BL MA> F:BG
<HFIHG>GM BG ;BH=B>L>E IKH=N<MBHG &M BL :ELH :G BFIHKM:GM <HFIHG>GM
?HK @>G>K:MBG@ ;BH@:L :G= ;BHAR=KH@>G PA>K> BM G>>=L MH ;> AR=KHERS>=
BGMH ?:MMR :<B=L 2G=>K MA> :G>KH;B< <HG=BMBHG EBIB=L :K> BGBMB:EER AR
=KHERS>= BGMH @ER<>KHE PAB<A BL ?NKMA>K <HGO>KM>= MH EHG@ <A:BG ?:MMR
:<B=L ;R MA> :<MBHG H? >QMK:<>EENE:K EBI:L>L 89
5. Factors a*ecting biological pretreatment
&G HK=>K MH :<AB>O> MA> F:QBFNF >?i<B>G<R H? - DGHPE>=@> HG
MA> ?:<MHKL :??><MBG@ FB<KH;B:E @KHPMA :G= F>M:;HEBLF BL O>KR BFIHKM:GM
89 1A> HNM<HF>L H? - BL :??><M>= ;R O:KBHNL ?:<MHKL PAB<A :K> FHK>
:II:K>GM BG ?NG@:E HK ;:<M>KB:E IK>MK>:MF>GM 1A> I:K:F>M>KL :??><MBG@
- <:G ;> K>E:M>= MH ;BHF:LL HK LN;LMK:M>L +- G:MNK> H? ;BHF:LL I:K
MB<E> LBS> :G= FHBLMNK> <HGM>GM ;BH:@>GML +- MRI>L H? FB<KHHK@:G
BLF HK >GSRF>L :G= BGH<NENF <HG<>GMK:MBHG :G= IKH<>LL <HG=BMBHGL
+- IK>MK>:MF>GM MBF> M>FI>K:MNK> I% :G= :>K:MBHG K:M> 1A> G:MNK>
H? ;BHF:LL BL HG> H? MA> IKBF:KR ?:<MHKL PAB<A =B??>K GHM HGER ?HK BML
<A>FB<:E <HFIHLBMBHG ;NM :ELH ?HK ;HM:GB<:E HKB@BG :L =BL<NLL>= >:KEB>K
0><MBHG  1RI>L H? FB<KHHK@:GBLFL :G= LMK:M>@B>L H? NLBG@ MA> ;BH
EH@B<:E :@>GML @K>:MER :??><M MA> >?i<B>G<R H? - PAB<A P>K> =BL<NLL>=
BG MA> IK>OBHNL L><MBHG 0><MBHG 
0NK?:<>L H? ;BHF:LL <HGLBLM H? >QM>KG:E :G= BGM>KG:E :K>: PA>K> MA>
LA:I> :G= LBS> H? I:KMB<E>L :K> BFIHKM:GM ?HK F:BGM:BGBG@ MA> LNBM:;E>
>QM>KG:E LNK?:<> :K>: :G= <:IBEE:KR LMKN<MNK> H? ;BHF:LL <HFIHG>GML
89 0F:EE I:KMB<E> LBS>L <HNE= ;> =>LBK>= :L BM IKHOB=>L BG<K>:L>= =B
@>LMB;BEBMR :G= iG:E RB>E= ;R >GA:G<BG@ MA> LNK?:<> :K>: %HP>O>K =B?
i<NEMB>L BG MA> =HPGLMK>:F IKH=N<M K><HO>KR BL :G BLLN> BG MA> NL:@> H?
LF:EE LBS> I:KMB<E>L 89 &G ?:<M MHH LF:EE I:KMB<E> LBS> BL GHM ?:OHK:;E>
?HK :<AB>OBG@ : ;>MM>K >?i<B>G<R :L MA> LF:EE>K BGM>KI:KMB<E> LI:<> :?
?><M FB<KH;B:E @KHPMA :G= F>M:;HEBLF ;R K>=N<BG@ MA> K:M> :G= :FHNGML
H? :>K:MBHG 89 !NKBG@ - H? ;BHF:LL :>K:MBHG BL BFIHKM:GM ?HK HQR
@>G:MBHG INKIHL> ,p K>FHO:E A>:M =BLLBI:MBHG ANFB=BMR F:BGM>G:G<>
:G= =BLMKB;NMBHG H? OHE:MBE> <HFIHNG=L IKH=N<>= ;R FB<KH;B:E F>M:;H
EBLF 89 ,G MA> HMA>K A:G= : E:K@>K I:KMB<E> LBS> :??><ML MA> >?i<B>G<R
H? MA> IK>MK>:MF>GM IKH<>LL ;R EBFBMBG@ FB<KH;B:E I>G>MK:MBHG BGMH MA>
LN;LMK:M>L :G= =><K>:LBG@ MA> NGB?HKF =B??NLBHG H? :BK MAKHN@AHNM MA>
LRLM>F
-K>MK>:MF>GM MBF> BL HG> H? MA> FHLM BFIHKM:GM ?:<MHKL BG - %HP
>O>K MA> HIMBFNF IK>MK>:MF>GM MBF> F:R O:KR BG K>LIHGL> MH MA> MRI>L
H? ;BHEH@B<:E :@>GML FB<KH;B:E <NEMNK>L HK >GSRF>L FB<KH;B:E LMK:BGL
;BHF:LL K><:E<BMK:G<> :G= IKH<>LL <HG=BMBHGL #HK >Q:FIE> F:QBFNF
LN@:K RB>E= P:L H;M:BG>= =NKBG@ AR=KHERLBL H? KB<> LMK:P IK>MK>:M>=
PBMA  59:8+':;9 :?M>K  = 89 PA>K>:L LN@:K RB>E= =B= GHM BG
<K>:L> LB@GB?B<:GMER :?M>K  = =NKBG@ IK>MK>:MF>GM H? <HKG LMHO>K PBMA
: ?NG@:E LMK:BG  9;(<+83/9658' 89 ,G MA> HMA>K A:G= LN@:K <HG
<>GMK:MBHG P:L ?HNG= MH ;> =><K>:L>= :?M>K  = PA>G PA>:M LMK:P
P:L IK>MK>:M>= PBMA  ).8?959658/;3 89 &G <HGMK:LM :G BG<K>:L>
BG LN@:K RB>E= P:L K><HK=>= NGMBE  = =NKBG@ IK>MK>:MF>GM

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
Fig. 9. *><A:GBLF H? =>@K:=BG@ MAK>> BFIHKM:GM ;BHIHERF>KL BG FB<KH:E@:> ;R MA> :<MBHG H? AR=KHERMB< >GSRF>L  LM:K<A  <>EENEHL> :G=   IKHM>BG />=K:PG :G= FH=Bi>= ?KHF
89
H? PHH= <ABIL PBMA " <+89/)5258 89 &G @>G>K:E BG<N;:MBHG MBF> PBEE
;> FN<A EHG@>K BG ?NG@:E IK>MK>:MF>GM P>>DL MH FHGMAL <HFI:K>= MH
MA> HMA>K :IIKH:<A>L
*HBLMNK> <HGM>GM BG MA> ;BHF:LL BL :GHMA>K <KBMB<:E ?:<MHK :G= LN?
i<B>GM E>O>E H? FHBLMNK> BL G><>LL:KR ?HK : A>:EMAR FB<KH;B:E @KHPMA
:G= ;BH=>@K:=:MBHG :<MBOBMR 89 %HP>O>K MA> HIMBFNF FHBLMNK>
<HGM>GM K>JNBK>= ?HK - O:KR =>I>G=BG@ HG MA> MRI> H? FB<KHHK@:GBLF
LI><B>L LMK:BG :G= ;BHF:LL 89 1A> ;:<M>KB:E :G= ?NG@:E <>EEL @>G
>K:EER @KHP P>EE PBMA MA>BK :<MBO> F>M:;HEBLF NG=>K : FHBLMNK> E>O>E
;>MP>>G  :G=  89 "Q<>LLBO> FHBLMNK> <HGM>GM =><K>:L>L BG
M>K I:KMB<E> LI:<>L :G= IHKHLBMR H? MA> LN;LMK:M>L PAB<A LN;L>JN>GMER
K>=N<>L HQR@>G =B??NLBHG MAKHN@AHNM MA> LRLM>F :G= BGAB;BML :>KH;B<
@KHPMA H? FB<KHHK@:GBLFL ,G MA> HMA>K A:G= MHH EHP E>O>E H? P:M>K
<:NL>L =>E:R>= @KHPMA H? FB<KH;B:E <>EEL =NKBG@ IK>MK>:MF>GM 89
1>FI>K:MNK> BL HG> H? MA> FHLM BFIHKM:GM IKH<>LL I:K:F>M>KL BG
- MA:M BL K>JNBK>= MH ;> F:BGM:BG>= :M MA> HIMBFNF E>O>E ?HK :<AB>O
BG@ IKHI>K @KHPMA :G= F>M:;HEB< :<MBOBMB>L H? <>EEL =NKBG@ - !>
I>G=BG@ HG MA> FB<KHHK@:GBLFL HIMBFNF M>FI>K:MNK>L ?HK 4/# O:K
B>= ;>MP>>G  ` :G=  `  L<HFR<>M>L ?NG@:E LI><B>L <:G @KHP :M :
AB@A>K M>FI>K:MNK> NI MH G>:KER  ` PABE> MA> @KHPMA M>FI>K:MNK>L
?HK ;:LB=BHFR<>M>L LMK:BGL K:G@>= ;>MP>>G  :G=  ` 89 1A>
K>:LHGL ?HK O:KB:MBHGL BG @KHPMA :G= IK>MK>:MF>GM M>FI>K:MNK>L H? =B?
?>K>GM ?NG@B <HNE= ;> =N> MH MA> O:KB:MBHG BG ?NG@:E IARLBHEH@R LMK:BGL
:G= MRI>L H? LN;LMK:M>L 89 1A> F>M:;HEBLF H? 4/# =NKBG@ LHEB=LM:M>
<NEMBO:MBHG NLN:EER @>G>K:M>L A>:M PAB<A BG MNKG BG<K>:L>L MA> M>FI>K
:MNK> @K:=B>GM BG MA> ;BHK>:<MHK 89 1ABL BL : D>R <A:EE>G@> BG MA> =>
LB@GBG@ H? ;BHK>:<MHK ?HK :IIEB<:MBHG H? LHEB=LM:M> IK>MK>:MF>GM HG E:K@>
L<:E>
1A> I% BG <NEMNK> F>=BNF :??><ML @KHPMA H? FB<KHHK@:GBLFL :G=
L><K>MBHG H? >GSRF>L *HLM H? MA> 4/# NLN:EER @KHP :M LEB@AMER :<B=B<
I%  89 I% <:G ;> =><K>:L>= :?M>K : ?>P =:RL H? FB<KH
;B:E @KHPMA 89 ,G MA> HMA>K A:G= jN<MN:MBHGL BG I% BG MA> F>=BNF
@K>:MER :??><M MA> IKH=N<MBHG ! LMKN<MNK>L :G= :<MBOBMR H? EB@GBGHERMB<
:G= AR=KHERMB< >GSRF>L 89 4BMA :G >Q<>IMBHG LHF> AB@A EB@GBG
=>@K:=BG@ ?NG@B EBD> $'8'8/' +,,;9)':' :G= /).53/:;9 97;'2+49 P>K> K>
IHKM>= ?HK IKH=N<BG@ EB@GBGHERMB< >GSRF>L PBMA : AB@A>K :<MBOBMR NG=>K
EHP I% <HG=BMBHGL 89

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
6. Research progresses and developments
1A> =HPGLMK>:F RB>E= BL MA> IKBF:KR BG=B<:MHK ?HK >O:EN:MBG@ MA> >?
i<B>G<R H? - &G I:KMB<NE:K - H? )  ?:<BEBM:M>L LN;L>JN>GM AR=KHERLBL
:G= ?>KF>GM:MBHG ?HK @>G>K:MBG@ MA> M:K@>M ;BH?N>EL - FHLMER PHKDL BG
)  ;R K>FHOBG@ EB@GBG <HGM>GM ?KHF MA> ;BHF:LL MA:M >GA:G<>L IHK>
LBS> H? MA> ;BHF:LL :G= IKHOB=>L >GE:K@>= LNK?:<> :K>: ?HK >GA:G<>=
:<<>LLB;BEBMR H? MA> AR=KHERMB< >GSRF>L <>EENE:L>L :G=HK QRE:G:L>L
89 %R=KHERLBL H? MA> <>EENEHL> :G=HK A>FB<>EENEHL> H? MA> IK>
MK>:M>= ;BHF:LL K>LNEM BG MA> H;M:BGF>GM H? LHEN;E> LN@:KL MA:M :K> LN;
L>JN>GMER ?>KF>GM>= BGMH ;BH?N>EL )BD>PBL> FB<KH:E@:> ;BHF:LL BL LN;
C><M>= MH MA> =HPGLMK>:F ?>KF>GM:MBHG HK :G>KH;B< =B@>LMBHG MH IKH=N<>
K>LI><MBO> ;BH?N>EL 1A> =HPGLMK>:F RB>E=L H? LHF> )  :G= FB<KH:E@:>
H;M:BG>= :?M>K IK>MK>:MF>GM :K> LNFF:KBS>= BG 1:;E>  &M <:G ;> GHM>=
MA:M MA> BG<K>:L> BG ;BH?N>E RB>E= =N> MH MA> :IIEB<:MBHG H? - BL FN<A
AB@A>K BG FB<KH:E@:> MA:G BG )  #HK >Q:FIE> BKK>LI><MBO> H? MA> -
:IIKH:<A :G= ;BHF:LL MA> F:QBFNF BG<K>:L> BG F>MA:G> RB>E= :?M>K -
H? )  P:L K>IHKM>= MH ;>  PAB<A P:L  BG FB<KH:E@:> 1:;E>

1A> =HPGLMK>:F RB>E=L <:G O:KR =>I>G=BG@ HG MA> IK>MK>:MF>GM
:IIKH:<A>L ;BHF:LL LMKN<MNK> :G= <HFIHLBMBHG :G= MA> IKH<>LL <HG
=BMBHGL &M BL MANL =B?i<NEM MH <HFI:K> MA> iG:E RB>E= <HG<ENLBO>ER NGE>LL
:EE MA> ?:<MHKL :K> M:D>G BGMH <HGLB=>K:MBHG HK : <HFIK>A>GLBO> HIMBFNF
<HG=BMBHG <:G ;> >LM:;EBLA>= +>O>KMA>E>LL MA> F:BG ?H<NL H? - HK HMA>K
IK>MK>:MF>GM F>MAH=L BL MH BG<K>:L> MA> =HPGLMK>:F RB>E= H? MA> M:K@>M
;BH?N>EL <<HK=BG@ER MA> BG<K>:L>= =HPGLMK>:F RB>E= :G= M><AGH><H
GHFB< OB:;BEBMR K>F:BG :L MA> ;:LBL ?HK :EFHLM :EE MA> K><>GM K>L>:K<A >?
?HKML HG - L BL LNFF:KBS>= BG 1:;E>  MA> K><>GM K>L>:K<A IKH@K>LL>L
BG - <:G ;> <:M>@HKBS>= BGMH MAK>> ;:LB< :LI><ML LN<A :L BLHE:MBHG :G=
L<K>>GBG@ H? MA> IHM>GM ;BH:@>GML HK LNBM:;E> ;BHF:LL M><AGH><HGHFB<
BFIKHO>F>GML :G= HIMBFBS:MBHG H? MA> IK>MK>:MF>GM <HG=BMBHGL
,G> H? MA> F:CHK :G= ;:LB< K>L>:K<A MK>G=L BG - BL MA> BLHE:MBHG
:G=HK L<K>>GBG@ H? IHM>GM EB@GBGHERMB< :G= AR=KHERMB< LMK:BGL :L >B
MA>K BG=BOB=N:E LMK:BGL 89 HK FB<KH;B:E <HGLHKMB: 89 G
HMA>K BGM>K>LMBG@ :G= IKHFBLBG@ K>L>:K<A >??HKM P:L F:=> K><>GMER HG
MA> NMBEBS:MBHG H? KNFBG:GM FB<KHjHK: ?HK MA> IK>MK>:MF>GM H? FB<KH:E
@:> ;BHF:LL 89 /NFBG:GM FB<KHjHK: :K> MA> G:MNK:E AR=KHERMB<
;:<M>KB:E <HGLHKMB: ?HNG= BG MA> =B@>LMBO> LRLM>FL H? M>KFBM>L :G= KN
FBG:GML 89 #HK >Q:FIE> :KK:@TG1KBGB=:= >M :E 89 K>IHKM>=
: LB@GB?B<:GM K>=N<MBHG BG F>MA:G> IKH=N<MBHG MBF> ;R MA> IK>=HFB
G:GM KNFBG:GM ;:<M>KB: EBD> 259:8/*/;3 85:+5)':+22' '4* 9+;*5354'9
&G :==BMBHG MH L<K>>G HNM IHM>GM FB<KHHK@:GBLFL L<K>>G
Table 5
0NFF:KR HG MA> =HPGLMK>:F IKH=N<M RB>E=L ?HK LHF> ;BHF:LL :?M>K ;BHEH@B<:E IK>MK>:MF>GML
BHF:LL
#BG:E
IKH=N<M -K>MK>:MF>GM:IIKH:<A
-K>MK>:MF>GM
<HG=BMBHGL
1>FIMBF> !HPGLMK>:FRB>E=L
HFI:KBLHGPBMANGMK>:M>=
;BHF:LL />?

0:P=NLM BH@:L:G=
F>MA:G>
*B<KH;B:E<HGLHKMBNFR>:LM:G=
;:<M>KB:ELMK:BGL
 ` = BH@:LRB>E=  )D@ 30
*>MA:G>RB>E=  )D@ 30
AB@A>K;BH@:LRB>E=
 AB@A>K F>MA:G>
RB>E=
89
/B<>LMK:P *>MA:G> #NG@:E2+;85:;959:8+':;9  ` = *>MA:G>RB>E=  )D@ 30  AB@A>K F>MA:G> RB>E= 89
/B<>LMK:P *>MA:G> #NG@:E"8/).5*+83'8++9+/ ` = *>MA:G>RB>E=  )D@ 30  AB@A>K F>MA:G>
RB>E=
89
HKGLM:ED 0N@:K #NG@:E.'4+85).'+:+
).8?959658/;3
 ` = *:Q L:<<A:KBi<:MBHG RB>E=

AB@A>KRB>E= 89
HKGLMHO>K "MA:GHE #NG@:E+8/658/569/9
9;(<+83/9658'
 ` = *:Q@EN<HL>RB>E=
*:Q>MA:GHERB>E=
AB@A>K>MA:GHERB>E= 89
0PBM<A@K:LL 0N@:K #NG@:E?)45658;9 LI  ` = *:Q =B@>LMB;BEBMR H? <>EENEHL>

AB@A>KLN@:KRB>E= 89
*BL<:GMANL $EN<HL> #NG@:E+8/658/569/9
9;(<+83/9658'
 ` = *:Q@EN<HL>RB>E=  MBF>LAB@A>KRB>E= 89
#BO>:@KB<NEMNK:E
K>LB=N>L
BG=BOB=N:EER
"MA:GHE "GLBEBG@PBMA>GSRF>L F;B>GM= "MA:GHE RB>E=  @@ <HMMHG
LM:ED MH  @@ MKBMB<:E>
A:R
 MKBMB<:E> A:R MH
 <HMMHG LM:ED AB@A>K
RB>E=
89
/)85'2-'+
);:5*+93;95(2/7;;9 *>MA:G> "GSRF:MB<<>EENE:L>IKHM>:L> β
@EN<:G:L>:G=QRE:G:L>
 `  A *>MA:G>RB>E=  FE@ 30 AB@A>KRB>E= 89
.258+22' LI *>MA:G> :<M>KB:E')/22;92/).+4/,583/9 `  A *>MA:G>RB>E=  FE@ 30 AB@A>KRB>E= 89
'445).258569/9
-'*/:'4'
*>MA:G> :<M>KB:E '5;2:+22'
584/:./452?:/)'
 `  A  AB@A>KRB>E= 89
!)+4+*+93;95(2/7;;9 *>MA:G> "GSRF:MB<<>EENE:L>:G=>G=H
@:E:<MHNKHG:L>
 `  A *>MA:G>RB>E=  FE@ 30 AB@A>KRB>E= 89
!)+4+*+93;95(2/7;;9 *>MA:G> "GSRF:MB<>LM>K:L>:G=IKHM>:L> `  A *>MA:G>RB>E=  FE@ 30 AB@A>KRB>E= 89
!)+4+*+93;95(2/7;;9 *>MA:G> "GSRF:MB<<>EENE:L>>LM>K:L>:G=
IKHM>:L>>G=H@:E:<MHNKHG:L>
 `  A *>MA:G>RB>E=  FE@ 30 AB@A>KRB>E= 89
*BQ>=FB<KH:E@:> *>MA:G> #NG@:E"8'3+:+9<+89/)5258 *>MA:G>RB>E=  FE@ 30 AB@A>KRB>E= 89
*BQ>=FB<KH:E@:> *>MA:G> "GSRF:MB<E:<<:L> *>MA:G>RB>E=  FE@ 30 AB@A>KRB>E= 89
.258+22'6?8+45/*59' 0N@:K:G=
EBIB=
"GSRF:MB< >EENE:L> `  A  BG<K>:L> BG <>EENEHL>
AR=KHERLBL
 BG<K>:L> BG EBIB= K><HO
>KR
89
.258+22'<;2-'8/9 %R=KH@>G "GSRF:MB<<KN=>>GSRF>L  `  A  FE %@<>EEL MBF>LAB@A>KRB>E= 89
.258+22'<;2-'8/9 %R=KH@>G
:G=
F>MA:G>
:<M>KB:E259:8/*/;3
:.+835)+22;3
 ` = AB@A>KRB>E= 89
30 3HE:MBE> LHEB=

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
Table 6
0HF> L>E><M>= K>L>:K<A IKH@K>LL>L :G= =>O>EHIF>GML BG MA> ;BHEH@B<:E IK>MK>:MF>GM =NKBG@
E:LM iO> R>:KL 
6>:K ,;C><MBO>L *:CHKiG=BG@L />F:KDL />?
 HF;BG>=
<A>FB<:E
+:p ,p :G=
;:<M>KB:E ;68/
'</*;9 ('9/2+49/9
 IK>MK>:M
F>GM H? KB<>
LMK:P
0N@:K RB>E= BF
IKHO>= ;R
 HO>K
LBG@E> +:p ,p
IK>MK>:MF>GM
0N@:K RB>E= BG
<K>:L>= ;R
?HE=L
HO>K NGMK>:M>=
KB<>LMK:P
 G>P LMK:M
>@R BG MA>
IK>MK>:MF>GM
H? ;BHF:LL NL
BG@ LRG>K@R H?
<A>FB<:E :G=
;:<M>KB:E
LMK:BG
89
 HF;BG>= :G=
BG=BOB=N:E IK>
MK>:MF>GM H?
<HKG LMHO>KL ;R
MPH ?NG@:E
LMK:BGL "8/).5
*+83'
.'8@/'4;3 :G=
96+8-/22;9 LI
>QMK:<M>= >G
SRF>L :G=
<HF;BG>= >G
SRF>L:ED:EB
+:,% ?HK
F>MA:G> IKH
=N<MBHG
*BQ>= ?NG@:E
IK>MK>:MF>GM =>
<K>:L>= F>MA:G>
IKH=N<MBHG ;R

*>MA:G> RB>E=
BG<K>:L>= ;R
 :?M>K :E
D:EB>GSRF> IK>
MK>:MF>GM
*BQ>= ?NG@:E
IK>MK>:MF>GM
F:R GHM ;>
@HH= ?HK
F>MA:G> IKH
=N<MBHG ;NM
FBQ>= >G
SRF>L <:G BG
<K>:L>
F>MA:G>
RB>E=
89
 -K>MK>:MF>GM H?
<HKG LMHO>KL NG
=>K MA>K
FHIABEB< 
`  :G=
3+95IABEB< 
`  <HG=BMBHGL
;R ')/22;9 9;(
:/22/9
*>LHIABEB< M><A
GBJN> <HGLNF>=
E>LL >G>K@R ;NM
RB>E= P:L K>E:
MBO>ER EHP>K
MA:G MA:M H;
M:BG>= ?KHF
MA>KFHIABEB<
IK>MK>:MF>GM
1A>KFHIABEB<
IK>MK>:MF>GM
BL ;>MM>K MA:G
3+95IABEB<
IK>MK>:MF>GM
B? AB@A>K >G
>K@R <HG
LNFIMBHG <:G
;> :==K>LL>=
IKHI>KER
89
 &LHE:MBHG :G=
B=>GMBi<:MBHG H?
EB@GBG =>@K:=
BG@ ;:<M>KB:
?KHF MA> KHMM>G
PHH= :G= :IIEB
<:MBHG BG MA>
IK>MK>:MF>GM H?
PA>:MLMK:P
1A> AB@A>LM
EB@GBGHERMB< =B
@>LMB;BEBMR P:L
LAHPG ;R ;81
.52*+8/' LI %
LMK:BG HNM H? 
BLHE:M>L
HFI:K>= MH
?NG@B ;:<M>K
B:E IK>MK>:M
F>GM BL
LAHKM>K :G=
>:LR MH FH=
B?R @>G>MB
<:EER 0H G>P
;:<M>KB:E
LMK:BGL PHNE=
;> IKHFBLBG@
?HK - H? ) 
89
 !>EB@GBi<:MBHG
H? LM>:F IK>
MK>:M>= IHIE:K
;R : LF:EE E:<
<:L>L L):< BLH
E:M>= ?KHF 3?
)52':569/9 LI
/>E>:L> H? :<B=
IK><BIBM:;E>
IHERF>KB< EB@GBG
BG<K>:L>= ;R
?HE=L PABE>
:<B=LHEN;E>
EB@GBG JN:GMBMR
=><K>:L>= ;R

L):< MH@>MA>K
PBMA <>EENE:L>
BG<K>:L>=  H?
@EN<HL>RB>E=
1ABL BL MA>
iKLM K>IHKM HG
=>EB@GBi<:
MBHG H? L):<
HG PHH=R
;BHF:LL
L):< BL
IKHFBLBG@
=N> MH MA> LM:
;BEBMR MH :
PB=> K:G@> H?
I% :G= M>F
I>K:MNK> LBF
IE> IKH=N<
MBHG :G= >:LR
>G@BG>>KBG@
89
Table 6 54:/4;+*
6>:K ,;C><MBO>L *:CHKiG=BG@L />F:KDL />?
 IIEB<:MBHG H?
KNFBG:E jNB= :L
AR=KHERMB< ;:<
M>KB:E LHNK<> BG
MA> IK>MK>:M
F>GM H? FBQ>=
FB<KH:E@:E ;BH
F:LL ?HK
F>MA:G> IKH
=N<MBHG
*:CHK ;:<M>KB:E
@>G>K: BG MA> KN
FBG:E jNB= P>K>
259:8/*/;3 85
:+5)':+22' :G=
9+;*5354'9
-K>MK>:MF>GM
:G= F>MA:G>
@>G>K:MBHG P>K>
<HFIE>M>= ;R
HGER  = PBMA
F:QBFNF
F>MA:G> RB>E= H?
 FE %p @
,!
/NFBG:GM
;:<M>KB:
<HNE= AHE=
FN<A IHM>G
MB:E ?HK
IKHFBLBG@
IK>MK>:MF>GM
H? I:KMB<N
E:KER FB<KH:E
@:>;BHF:LL
89
 0<K>>GBG@ H?
?HNK DGHPG
:@KB<NEMNK:E
K>LB=N>L <HKG
LMHO>K ;:KE>R
LMK:P <HKG<H;
:G= PA>:M
LMK:P ?HK ?NG
@:E 86+> 2')
:+;9 IK>MK>:M
F>GM
HKG LMHO>K
LAHP>= MA> ;>LM
K>LNEM BG M>KFL H?
EB@GBG K>FHO:E
F:Q 
LN@:K EHLL FBG
 :G= L>
E><MBOBMR O:EN>
F:Q
!HPGLMK>:F =B
@>LMB;BEBMR H? <:K
;HAR=K:M>L BG
<K>:L>= ;R

1A> ?HNK ;BH
F:LL>L P>K>
L>E><M>=
;:L>= HG
MA>BK <HFIH
LBMBHG :G= @>
H@K:IAB<
:O:BE:;BEBMR
HKG LMHO>K
<HNE= ;> : IH
M>GM F:M>KB:E
?HK-
89
 0<K>>GBG@ ?HK
L>E><MBO> EB@GBG
=>@K:=BG@ ?NG
@:E LMK:BGL ?KHF
 4/# :G=
IK>MK>:MF>GM H?
<HKGLMHO>K
1AK>> AB@A L>E><
MBO> 4/# LMK:BGL
P>K> L<K>>G>=
HNM G:F>ER ?
':.;9 9:+8)58+;9
?)45658;9 9'4
-;/4+;9 :G=
.2+(/' (8+</9
658'
0>E><M>= LMK:BGL
IKH=N<>=
 F@
@ H?LN@:KL
0>E><MBO>
EB@GBG =>@K:
=:MBHG BL MA>
IKBF:KR <HG
<>KG BG - H?
)  :G= MA>
MAK>> ?NG@:E
LMK:BGL
LAHP>= =>LBK
:;E> L>E><MBO
BMR
89
 "O:EN:MBHG H?
MA> ?>:LB;BEBMR H?
?NG@:E IK>MK>:M
F>GM "8'3+:+9
./89;:+ ?HK KB<>
LMK:PL =NKBG@
;BH>MA:GHE IKH
=N<MBHG
HFI:KBLHG H?
?NG@:E IK>MK>:M
F>GM PBMA
LM>:F IK>MK>:M
F>GM
>EENEHL> K><HO
>KR P:L LEB@AMER
EHP>K BG ?NG@:E
 MA:G BG
LM>:F IK>MK>:M
F>GM
)B@GBG K>FHO:E
P:L LB@GBi<:GMER
AB@A>K PBMA ?NG
@:E K>LB=N:E
 MA:G BG
LM>:F IK>MK>:M
F>GM K>LB=N:E

"MA:GHE <HG<>G
MK:MBHG P:L EHP
BG ;HMA IK>MK>:M
F>GML ;NM ?NG
@:E IK>MK>:MF>GM
P:L <HFI>MBMBO>
PBMA LM>:F BG
>MA:GHE IKH=N<
MBHG
0M>:F IK>
MK>:MF>GM
G>>=>=
A:KLA>K <HG
=BMBHGL
 ILB :G=
`  MA:G
?NG@:E IK>
MK>:MF>GM
PABE> MA> E:M
M>K K>JNBK>=
EHG@>K MBF>
 = MA:G MA>
?HKF>K
 FBG
89

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
Table 6 54:/4;+*
6>:K ,;C><MBO>L *:CHKiG=BG@L />F:KDL />?
 #NG@:E 
9;(<+83/9658'
:G= ;:<M>KB:E
'):5(')/22;9
(;).4+8/ >G
LBEBG@ P>K>
<HFI:K>= BG
=BOB=N:EER :G=
BG <HF;BG>=
=NKBG@
;BH>MA:GHE
:G= ;BH@:L
IKH=N<MBHG
HF;BG>= ?NG@:E
:G= >GLBEBG@ BF
IKHO>= HO>K:EE
>MA:GHE IKH=N<MBHG
;NM K>JNBK>= EHG@
MBF>G>M=
HF;BG>= F>MAH=
P:L GHM >m><MBO>
?HK ;BH@:L IKH=N<
MBHG :L F>MA:G>
RB>E= =Bm>K>= BG
LB@GBi<:GMER ;>
MP>>G BG=BOB=N:E
:G= <HF;BG>=
F>MAH=L
HF;BG:MBHG
H? ?NG@:E :G=
;:<M>KB:E >G
LBEBG@ <HNE=
;> @HH= ?HK
;BH>MA:GHE
IKH=N<MBHG
;NM GHM ?HK
;BH@:L
89
 .N:GMBi<:MBHG
H? @>GHFB<
!+ P:L
=HG> BG MABL
LMN=R ;R J
- / NLBG@
LMK:BGL MH
JN:GMB?R ?NG
@:E <>EEL BG
LHEB= LM:M> <NE
MNK>
1A> J- / M><A
GBJN> LAHP>= BML
IHM>GMB:E MH =>M><M
FR<>EB:E @KHPMA H?
MA>?NG@:ELMK:BGL
1A> FBGBFNF =>
M><MBHG EBFBM O:KB>=
;>MP>>G  :G=
 μ@ FR<>EBNF
@ ;BHF:LL =KR
P>B@AM
*HGBMHKBG@
H? FB<KH;B:E
@KHPMA =NK
BG@ IK>MK>:M
F>GM BL BF
IHKM:GM
*HGBMHKBG@
H? ?NG@:E
@KHPMA BL
>:LR ?HK LN;
F>K@>= <NE
MNK> ;NM
IKH;E>F:MB<
?HK LHEB=
LM:M><NEMNK>
1A> J- /
M><AGBJN>
<HNE= ;> NL>
?NE ?HK FHG
BMHKBG@
@KHPMA BG
LHEB= LM:M>
<NEMNK>
89
 "O:EN:MBHG H?
 =Bm>K>GM
?NG@B ?HK IK>
MK>:MF>GM H?
LPBM<A @K:LL BG
M>KFL H? <H
IKH=N<MBHG H?
EB@GBGHERMB<
>GSRF>L :G=
MA> >k<B>G<R
H? =HPG
LMK>:F AR
=KHERLBL
:L>= HG MA> M:K@>M
BG=B<>L :G BG>=B;E>
FNLAKHHF G:F>ER
-'8/);9 352?(*/:+9
P:L ?HNG= IKHFBL
BG@ ?HK IK>MK>:M
F>GM H? LPBM<A
@K:LL
1A> ?NG@B L><K>M>=
>B@AM :<<>LLHKR >G
SRF>L PAB<A ?:<BE
BM:M> <>EENE:L> =B
@>LMB;BEBMR
1H @>M :G >?
i<B>GM AR
=KHERLBL H?
LPBM<A @K:LL
: <HF;BG:
MBHG H? ?NG
@:E IK>MK>:M
F>GM :G= AR
=KHERLBL PBMA
:==BMBHG H?
<>EENE:L> :G=
LRG>K@BLMB<
>GSRF>L
<HNE= ;> :
IKHFBLBG@
LRLM>F
89
Table 6 54:/4;+*
6>:K ,;C><MBO>L *:CHKiG=BG@L />F:KDL />?
 "O:EN:MBHG H?
EB@GBGHERMB< :G=
AR=KHERMB< >G
SRF>L ?KHF MA>
<KN=> >GSRF>L
H;M:BG>= ?KHF :
<H<NEMNK> H?
568/4;9 )53'
:;9 :G= "8/).5
*+83'8++9+/
1A> :<MBOBMR H?
EB@GH<>EENEHERMB<
>GSRF>L P:L BG
<K>:L>= ;R MA>
<H<NEMNK> <HF
I:K>= MH LBG@E>
<NEMNK> H? MA>
?NG@B
*:QBFNF :<MBO
BMR H? E:<<:L> >G
A:G<>= ;R
MBF>L :?M>K 
= >:KEB>K MA:G
MA> :<MBOBMR :G=
MBF> H? LBG@E>
<NEMNK> H?  )5
3':;9
1A> AB@A>LM
:FHNGML H?
EB@GBG K>FHO:E
:G= =HPGLMK>:F
LN@:K RB>E= P>K>
:<AB>O>= ;R
 :G= 
K>LI><MBO>ER
- K>JNBK>L :
LN;L>JN>GM
AR=KHERLBL
LM>I MA:M >F
IEHRL <HLMER
AR=KHERMB< >G
SRF>L PAB<A
<:G ;> FBGB
FBS>= ;R NL
BG@ EB@GB
GHERMB< :G=
AR=KHERMB< >G
SRF>L MH
@>MA>K H;
M:BG>= ?KHF :
<H<NEMNK>
LRLM>F
89
 "O:EN:MBHG H?
MA> >m><ML H?
<A>FB<:E ?:<
MHKL HG MA> BGM>
@K:M>= ?NG@:E
?>KF>GM:MBHG
&## H? LN@:K
<:G> ;:@:LL>
NLBG@ .2+(/' LI
*$
&GBMB:E FHBLMNK>
:m><M>= EB@GBG
K>FHO:E >k
<B>G<R PA>K>
 FHBLMNK>
P:L ?HNG= MH ;>
HIMBF:E ?HK
=>EB@GBi<:MBHG
:G= >MA:GHE IKH
=N<MBHG
0NIIE>F>GM:
MBHG H? ;:L:E
F>=BNF BG
<K>:L>= EB@GBG
K>FHO:E :G=
>MA:GHE IKH=N<
MBHG
0>O>K:E =BO:E>GM
BGHK@:GB< <A>F
B<:EL #>p
*Gp Np =>
<K>:L>= AHEEH<>E
ENEHL> EHLL =NK
BG@ IK>MK>:M
F>GM :G= ?NK
MA>K >GA:G<>=
>MA:GHERB>E=
-KH=N<MBHG
:G= :<MBOBMR
H? EB@GBGHERMB<
>GSRF>L :K>
:m><M>= ;R
L>O>K:E <A>F
B<:E ?:<MHKL
PAB<A G>>= MH
HIMBFBS> ?HK
>k<B>GM-
&## P:L LMN=
B>= ?HK MA>
iKLM MBF> MA:M
<:KKB>= HNM
=>EB@GBi<:
MBHG :G= <HG
LHEB=:M>= ;BH
IKH<>LLBG@
:EHG>
89
BG@ H? LNBM:;E> ;BHF:LL ?HK - :G= ;BH?N>E IKH=N<MBHG BL :GHMA>K K><>GM
K>L>:K<A MK>G= 89 ><:NL> MA> >?i<B>G<R H? FB<KH;B:E IK>MK>:M
F>GM GHM HGER =>I>G=L HG MA> ;BH:@>GML ;NM :ELH HG MA> MRI> H? ;BH
F:LL =N> MH MA> O:KB:MBHGL BG <HFIHLBMBHG :G= LMKN<MNK>L H? MA> ;BHF:LL
89 #HK BGLM:G<> : L<K>>GBG@ H? ;BHF:LL P:L K><>GMER =HG> NLBG@ ?HNK
PB=>ER :O:BE:;E> :@KB<NEMNK:E K>LB=N>L MH >O:EN:M> MA> O:KB:MBHG BG MA> K>
LIHGL> H? ?NG@:E @KHPMA :G= =>EB@GBi<:MBHG ?HK ;HM:GB<:E HKB@BGL H? ;BH
F:LL 89
1><AGHEH@B<:E BFIKHO>F>GM BG - BL NMFHLM BFIHKM:GM ?HK H;M:BG
BG@ : ;>MM>K >?i<B>G<R PBMA : AB@A>K =HPGLMK>:F RB>E= &G MABL K>
@:K= HG> H? MA> GHM>PHKMAR M><AGHEH@B<:E IKH@K>LL>L BG K><>GM MBF>L
BL MA> <HF;BG:MBHG H? - PBMA HMA>K MK:=BMBHG:E IK>MK>:MF>GM F>MA
H=L 1A> <HG<>IM H? LN<A <HF;BG:MBHG A:L ;>>G BGO>LMB@:M>= =NKBG@
MA> E:LM L>O>K:E R>:KL 89 HF;BG:MBHG H? - <:G ;> =HG> ;R
O:KBHNL P:RL LN<A :L ;BHEH@B<:EIARLB<:E 89 ;BHEH@B<:E<A>F
B<:E 89 ;BHEH@B<:EIARLB<H<A>FB<:E 89 :G= >O>G ;BH
EH@B<:E;BHEH@B<:E 89 1A> F:CHK :=O:GM:@>L H? <HF;BG>= IK>MK>:M
F>GM HO>K MA> LBG@E> - :K> K>E:MBO>ER AB@A>K EB@GBG K>FHO:E :G= =B

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
@>LMB;BEBMR H? MA> ;BHF:LL LAHKM>K IK>MK>:MF>GM MBF> EHP>K <:K;HAR
=K:M>L EHLL :G= AB@A>K =HPGLMK>:F RB>E=L 89 1H :<AB>O> M><AGH><H
GHFB< LNLM:BG:;BEBMR BG - :GHMA>K IKHFBLBG@ M><AGB<:E :LI><M <HNE= ;>
LHEB=LM:M> <NEMNK> MA:M H??>KL ?>:LB;BEBMR MH <HG=N<M - NG=>K AB@A LHEB=
EH:= %HP>O>K FHGBMHKBG@ H? FB<KH;B:E @KHPMA =NKBG@ IK>MK>:MF>GM BL
BFIHKM:GM :G= BM BL GHM :G >:LR CH; LN<A LRLM>F <HFI:K>= MH MA> LN;
F>K@>= <NEMNK> 1H :==K>LL MABL BLLN> JN:GMBi<:MBHG H? @>GHFB< !+
P:L =HG> ;R J- / ?HK JN:GMB?RBG@ MA> <>EEL BG LHEB= LM:M> <NEMNK> 89
"LM:;EBLAF>GM H? MA> HIMBFNF HI>K:MBHG:E <HG=BMBHGL BG - BL BFIHK
M:GM ?HK >GLNKBG@ BML M><AGH><HGHFB< ?>:LB;BEBMR ?HK MA> M:K@>M ;BH?N>EL
&G @>G>K:E - A:L ;>>G K><>BO>= BML K>L>:K<A BGM>K>LML ?HK FBE= HI>K
:MBG@ <HG=BMBHGL PABE> FBE= M>FI>K:MNK>L F:R <K>:M> IKH;E>FL BG MA>
<HGLHEB=:M>= :G:>KH;B< =B@>LMBHG =HG> NG=>K MA>KFHIABEB< <HG=BMBHGL
1H LHEO> MABL BLLN> MA>KFHIABEB<  `  IK>MK>:MF>GM P:L LMN=B>= NL
BG@ ;BH@:L LENKKB>L :L MA> BGH<NENF ?HK ;:<M>KB:E IK>MK>:MF>GM H? <HKG
LMHO>KL 89 &M P:L K>IHKM>= MA:M F>MA:G> RB>E= BG<K>:L>= ;R 
NG=>K MA>KFHIABEB< <HG=BMBHG <HFI:K>= MH MA> RB>E= H? NGMK>:M>= ;BH
F:LL 89 !>LIBM> MA> >??><MBO>G>LL H? MABL M><AGBJN> MA>K> :K> MPH
F:CHK BLLN>L PBMA MABL :IIKH:<A LN<A :L NMBEBS:MBHG H? NGDGHPG FB
<KHjHK: ?KHF MA> LENKKR :G= K>JNBK>F>GM H? AB@A M>FI>K:MNK>L 1A>K>
?HK> ?HK iG=BG@ : FHK> L>E><MBO> M><AGBJN> PBMA K>=N<>= >G>K@R <HG
LNFIMBHG FB<KH:>KH;B< IK>MK>:MF>GM H? LMHO>KL P:L <HG=N<M>= NG=>K
3+95IABEB< <HG=BMBHGL  `  NLBG@ : INK> ;:<M>KB:E LMK:BG ')/22;9 9;(
:/2/9 89 1ABL :IIKH:<A P:L ?HNG= MH ;> >?i<B>GM GHM HGER ?HK =><K>:L
BG@ MA> IK>MK>:MF>GM M>FI>K:MNK>L ;NM :ELH BM BG<K>:L>= F>MA:G> RB>E=
 >O>G AB@A>K MA:G MA> IK>OBHNL LMN=R  89 &G :G
HMA>K LMN=R : INK> ;:<M>KB:E LMK:BG G:F>ER ')/22;9 2/).+4/,583/9 P:L BG
O>LMB@:M>= =NKBG@ F>MA:G> IKH=N<MBHG MA:M K>IHKM>= LB@GB?B<:GM =><K>:L>
BG M>FI>K:MNK> :G= MBF> 89
7. Challenges and future perspectives
 .'22+4-+9 /4  5, 
!>LIBM> A:OBG@ MA> IHM>GMB:EL H? - BG ) ;:L>= ;BH?N>EL :G= ;BHK>
iG>KB>L >QIEHBM:MBHG H? MABL M><AGHEH@R HG E:K@> L<:E> BL LMBEE JN>LMBHG
:;E> FHLMER ?HK MAK>> <A:EE>G@>L EHG@ IK>MK>:MF>GM MBF> GHGL>E><MBO>
EB@GBG ;K>:D=HPG :G= K>E:MBO>ER EHP>K =HPGLMK>:F RB>E= <HFI:K>= MH
HMA>K <HGM>FIHK:KR MA>KFH<A>FB<:E F>MAH=L 1A> HNM<HF>L H? - F:R
O:KR =>I>G=BG@ HG MA> LMK:BGL :G= ;BHF:LL <HFIHLBMBHG =N> MH MA> O:KB
:MBHGL BG F>M:;HEB< >?i<B>G<R H? FB<KHHK@:GBLFL :G= =>@K>> H? I>KLBL
M>G<> H? MA> ;BHF:LL ,G MA> HMA>K A:G= LHF> LMN=B>L K>IHKM>= >O>G :
AB@A>K >?i<B>G<R H? - <HFI:K>= MH MA> <HGO>GMBHG:E M><AGBJN>L #HK
>Q:FIE> IK>MK>:MF>GM H? KB<> LMK:P ;R : ?NG@:E LMK:BG :G= LM>:F ;>
?HK> AR=KHERLBL :G= ?>KF>GM:MBHG ?HK ;BH>MA:GHE IKH=N<MBHG LAHP>= :
AB@A>K EB@GBG K>FHO:E :G= AR=KHERLBL >?i<B>G<R MA:G MAHL> IKHOB=>= ;R
MA> LM>:F IK>MK>:MF>GM 89 &GM>K>LMBG@ER >MA:GHE RB>E= P:L EHP>K BG
?NG@:E IK>MK>:M>= LMK:P MA:G BG LM>:F IK>MK>:M>= LMK:P 89 EMAHN@A
EB@GBG K>FHO:E :G= =HPGLMK>:F RB>E=L H? ?NG@:E IK>MK>:MF>GM :K> LHF>
MBF>L <HFI:K:;E> PBMA MA> MK:=BMBHG:E :IIKH:<A>L MA> MBF> K>JNBK>= ?HK
MA> E:MM>K BL FN<A LAHKM>K : ?>P FBGL MH AHNKL HGER 89 <HF
I:K>= MH MA> P>>DL MH FHGMAL H? MA> ?HKF>K 89
4ABE> ?NG@:E IK>MK>:MF>GM <HNE= ;> MA> ;>LM :IIKH:<A :FHG@ MA>
- ?HK IK>MK>:MF>GM H? )  89 EHG@ BG<N;:MBHG MBF> ;HMME>G><DL
BML >QIEHBM:MBHG HG E:K@> L<:E> &G <HGMK:LM ;:<M>KB:E @KHPMA BL ?:LM>K
:G= A>G<> IK>MK>:MF>GM MBF> BL LAHKM>K 89 ;NM RB>E= BL EHP>K BG ;:<
M>KB:E IK>MK>:MF>GM #HK >Q:FIE> IK>MK>:MF>GM H? MA> (K:?M EB@GBG ;R
: ;:<M>KB:E LMK:BG ;68/'</*;9 ('9/2+49/9  K>LNEM>=  EB@GBG K>
FHO:E :?M>K  =:RL 89 PABE> : ?NG@:E IK>MK>:MF>GM "8'3+:+9 ./8
9;:' H? <HKG LMHO>KL K>LNEM>= :L FN<A :L  K>FHO:E H? EB@GBG :?M>K
 =:RL 89 GHMA>K EBFBM:MBHG BG MA> - H? )  BL MA> GHGL>E><
MBO> EB@GBG K>FHO:E HK EHLL H? AHEEH<>EENEHL> 89 L =BL<NLL>= >:K
EB>K 0><MBHG  1:;E>  IK>MK>:MF>GM PBMA 4/# <:NL>=  EHLL
BG <>EENEHL> :G=  EHLL BG A>FB<>EENEHL> =>I>G=BG@ HG MA> ?NG@:E
LMK:BGL ;BHF:LL :G= IKH<>LL <HG=BMBHGL 1:;E>  EMAHN@A MA> EHLL H?
AHEEH<>EENEHL> >LI><B:EER A>FB<>EENEHL> BL :ELH H;L>KO>= BG MA> <HGO>G
MBHG:E F>MAH=L PBMA MA> ?HKF:MBHG H? O:KBHNL BGAB;BMHKL MA> EHLL H? <:K
;HAR=K:M>L L>>FL MH ;> AB@A>K BG ?NG@:E IK>MK>:MF>GM 1A> MABK= <A:E
E>G@> BG - BL K>E:MBO>ER MA> EHP>K >?i<B>G<R BG EB@GBG K>FHO:E :G= iG:E
RB>E= <HFI:K>= MH MA> MK:=BMBHG:E F>MAH=L #HK BGLM:G<> IK>MK>:MF>GM H?
LN@:K<:G> ;:@:LL> PBMA :ED:EB:<B=  +:,% :G=  I>K:<>MB< :<B=
K>LNEM>= : K>FHO:E H? :KHNG=  EB@GBG BG  A 89
1H HO>K<HF> MA> <A:EE>G@>L L>O>K:E >??HKML A:O> ;>>G =>OHM>= BG
K><>GM R>:KL #BKLMER - P:L <HF;BG>= PBMA HMA>K M><AGBJN>L MA:M <:G
K>=N<> HO>K:EE IK>MK>:MF>GM MBF> PBMA : AB@A>K >?i<B>G<R 0><MBHG 
#HK BGLM:G<> <HF;BG>= ?NG@:E :G= FBEEBG@ IK>MK>:MF>GM A:L K>LNEM>=
: LB@GB?B<:GM BFIKHO>F>GM BG =>EB@GBi<:MBHG H? KB<> LMK:P ?KHF 
?NG@:E MH  ?NG@:E :G= FBEEBG@ 89 &G :GHMA>K LMN=R <HF
;BG>= =BENM> :<B= :G= ;:<M>KB:E ;68/'</*;9 ('9/2+49/9  IK>MK>:MF>GM
H? KB<> LMK:P BG<K>:L>= =HPGLMK>:F >GSRF:MB< =B@>LMB;BEBMR ;R 
:G=  <HFI:K>= MH MA> HNM<HF>L H? :<B= IK>MK>:MF>GM :EHG>
:G= NGMK>:M>= KB<> LMK:P K>LI><MBO>ER 89 0><HG=ER NMBEBS:MBHG H? FB
<KH;B:E <HGLHKMB: <:G K>=N<> IK>MK>:MF>GM MBF> MH LHF> >QM>GM 89
1ABK=ER <KN=> HK INKBi>= EB@GBGHERMB< >GSRF>L P>K> NL>= ?HK IK>MK>:M
F>GM PAB<A M:D> L>O>K:E AHNKL MH =:RL 89 #HK >Q:FIE> IK>MK>:M
F>GM H? PA>:M LMK:P PBMA ?NG@:E "8'3+:+9 </2259' :G= ;:<M>KB:E !:8+6
:53?)+9 /6535+'+ E:<<:L>L MHHD HGER  A MH =>@K:=> EB@GBG >??><MBO>ER
PA>K> ;HMA MRI>L H? E:<<:L>L LAHP>= :EFHLM MA> L:F> >?i<B>G<R BG EB@GBG
;K>:D=HPG 89 #BG:EER L<K>>GBG@ :G= NMBEBS:MBHG H? IHM>GM FB<KH;B:E
LMK:BGL A:OBG@ AB@A L>E><MBO> O:EN> P>K> LN@@>LM>= ?HK FBGBFBSBG@ MA>
<:K;HAR=K:M> EHLL 89 )HLL H? <:K;HAR=K:M>L <:G ?NKMA>K ;> K>
=N<>= ;R HIMBFBSBG@ MA> IK>MK>:MF>GM <HG=BMBHGL :G= @>G>MB< FH=Bi<:
MBHG H? MA> @>G>L >G<H=BG@ EB@GBGHERMB< HK AR=KHERMB< >GSRF> 89
 .'22+4-+9 /4  5, 3/)85'2-'+
4ABE> >GSRF:MB< IK>MK>:MF>GM BL IK>=HFBG:GM BG MA> - H? FB<KH:E
@:> O:KB:MBHG BG MA> <>EE P:EE <HFIHLBMBHG :G= =>@K>> H? K><:E<BMK:G<>
:FHG@ MA> FB<KH:E@:> LI><B>L <:NL>L =B?i<NEMB>L MH H;M:BG : NGBO>KL:E
IK>MK>:MF>GM :IIKH:<A ?HK MA>L> ;BHF:LL>L #HK >Q:FIE> L>O>K:E FB
<KH:E@:> LI><B>L EBD> .258+22' ,;9)' :G= '45).258;3 +;)'8?5:;3'8+ :K>
LNKKHNG=>= ;R : IKHM><MBO> MKBE:FBG:K HNM>K P:EE DGHPG :L MKBE:FBG:K
LA>:MA MA:M BG<K>:L>L K>LBLM:G<> H? FB<KH:E@:E <>EE P:EEL 89 GHMA>K
K>LBLM:GM ;BHIHERF>K ?HNG= BG LHF> FB<KH:E@:> BL :E@:>G:G PAB<A BL
LHF>MBF>L >O>G GHGAR=KHERL:;E> 89 &G :==BMBHG FB<KH:E@:> @KHPG
I:KMB<NE:KER BG P:LM>P:M>K F:R ;> :LLH<B:M>= PBMA MA> ;:<M>KB:E jHK:
:G= <:G ?HKF jH< MA:M <HNE= ?NKMA>K BG<K>:L> <HFIE>QBMR BG MA> ;BHF:LL
<HFIHLBMBHG 89 GHMA>K <A:EE>G@> BG MA> >GSRF:MB< IK>MK>:MF>GM H?
FB<KH:E@:> BL MA> <HLML H? AR=KHERMB< >GSRF> <H<DM:BEL PAB<A <HNE= ;>
: F:CHK ><HGHFB< ABG=K:G<> BG BML E:K@>L<:E> >QIEHBM:MBHG 89 1A>
<HLML H? >GSRF>L <HNE= ;> FBGBFBS>= MH LHF> >QM>GM ;R BG<K>:LBG@ MA>BK
K>NL:;BEBMR MAKHN@A BFFH;BEBS:MBHG %HP>O>K K><R<EBG@ H? BFFH;BEBS>=
HK ?K>> >GSRF>L =><K>:L>L MA> :<MBOBMR :G= >?i<B>G<R H? MA> MK>:MF>GM
IKH<>LL &G :G >:KEB>K LMN=R #N >M :E 89 A:O> K>IHKM>= FHK> MA:G
 =><K>:L> BG MA> RB>E= H? AR=KHERLBL :?M>K iO> <R<E>L K><R<EBG@ H?
<>EENE:L> >GSRF>L EM>KG:MBO>ER NMBEBS:MBHG H? EBOBG@ AR=KHERMB< ;:<M>
KB: ?HK MA> IK>MK>:MF>GM H? FB<KH:E@:> P:L LN@@>LM>= :G= BGO>LMB@:M>=
89
!>LIBM> AR=KHERMB< ;:<M>KB: A:O> IHM>GMB:E ?HK FB<KH:E@:> IK>MK>:M
F>GM @>G>K:MBHG H? O:KBHNL IKH=N<ML PBMA EHP LI><Bi<BMR =NKBG@ MA>BK
@KHPMA :G= F>M:;HEBLF BL : @K>:M <A:EE>G@> ;><:NL> MA>L> IKH=N<ML
F:R <HFIEB<:M> MA> LN;L>JN>GM ;BH<HGO>KLBHG :G= =HPG

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
LMK>:F K><HO>KR LM>IL 89 +>O>KMA>E>LL BGM>K?>K>G<> H? LN<A ;RIKH=
N<ML BG MA> HO>K:EE ;BH<HGO>KLBHG IKH<>LL F:R =>I>G= HG MA> LMK:BGL HK
LI><B>L H? AR=KHERMB< ;:<M>KB: MA>BK :IIEB<:MBHG >BMA>K :L LBG@E> HK BG
<HGLHKMB: :G= @H:E H? MA> LN;L>JN>GM IKH<>LL LM>IL #HK >Q:FIE> :G:>K
H;B< IK>MK>:MF>GM H? .2'3?*5354'9 8+/4.'8*:// PBMA LBG@E> 259:8/*/;3
(;:?8/);3 ?HK LN;L>JN>GM AR=KH@>G IKH=N<MBHG K>LNEM>= BG MA> IKH=N<
MBHG H? LHF> ;RIKH=N<ML G:F>ER :<>MB< :<B= ?HKFB< :<B= IKHIBHGB<
:<B= :G= ;NMRKB< :<B= 89 1A>L> ;RIKH=N<ML =B= GHM F:D> :GR <HF
IEB<:MBHG K:MA>K MA> ;RIKH=N<ML P>K> NL>= ;R MA> FB<KHHK@:GBLFL MA:M
K>LNEM>= BG FHK> AR=KH@>G IKH=N<MBHG =NKBG@ IAHMHLRGMA>MB< ?>KF>G
M:MBHG &G <HGMK:LM IK>MK>:MF>GM H? .258+22' <;2-'8/9 NLBG@ AR=KHERMB<
;:<M>KB: ?HK HBE >QMK:<MBHG K>LNEM>= BG MA> IKH=N<MBHG H? LHEN;E> LN@:KL
PAB<A P>K> <HGLNF>= ;R MA> ;:<M>KB: 89 EMAHN@A <HGLNFIMBHG H?
LN@:KL ;R MA> AR=KHERMB< ;:<M>KB: P:L GHM <HGLB=>K>= :L : G>@:MBO> >??><M
=NKBG@ HBE >QMK:<MBHG MA> L:F> PHNE= ;> : F:CHK M><AGH><HGHFB< ABG
=K:G<> B? BM A:II>GL BG <:L> H? ?>KF>GM:MBO> ;BH?N>EL IKH=N<MBHG BG<EN=
BG@ ;BH:E<HAHEL ;BH@:L :G= ;BHAR=KH@>G 89 1H BFIKHO> MA> LI><Bi<
:<MBOBMR H? AR=KHERMB< ;:<M>KB: =NKBG@ IK>MK>:MF>GM :IIEB<:MBHG H? F>M:
;HEB< >G@BG>>KBG@ <HNE= ;> : OBM:E HIMBHG 1ABL :IIKH:<A <:G H??>K DGH<D
BG@ HNM LHF> @>G>L K>LIHGLB;E> ?HK ;RIKH=N<ML ?HKF:MBHG HK DGH<DBG@
BG H? LHF> M:K@>M @>G>L MH H;M:BG L>E><MBO> IK>MK>:MF>GM :L P>EE :L FBGB
FBS> MA> <HGLNFIMBHG H? MA> M:K@>M <HFIHG>GML =NKBG@ IK>MK>:MF>GM
 ;:;8+ 6+896+):/<+9
<<HK=BG@ MH MA> HO>K:EE =BL<NLLBHG H? MABL K>OB>P BFIE>F>GM:MBHG
H? - HG E:K@> L<:E> BL LMBEE ABG=>K>= ;R LHF> D>R M><AGH><HGHFB< BL
LN>L HGL>JN>GMER FHK> LMN=B>L :K> K>JNBK>= BG ?NMNK> ?HK M><AGHEH@B
<:E =>O>EHIF>GML BG - MAKHN@A <HG=N<MBG@ ;HMA ?NG=:F>GM:E :G= :I
IEB>= K>L>:K<A>L 1><AGHEH@B<:E :G= ><HGHFB< LNLM:BG:;BEBMR H? - <:G
:ELH ;> :<AB>O>= ;R BGM>@K:MBG@ - PBMA ;BHK>iG>KR :G= <HGLHEB=:M>=
;BHIKH<>LLBG@ <HG<>IML *HK>HO>K <HFIK>A>GLBO> L<:E> NI LMN=B>L PBMA
FH=>EEBG@ LBFNE:MBHG :G= ><HGHFB< :G:ERLBL LAHNE= @>M K>L>:K<A IKBHK
BMR MH H;M:BG IK:<MB<:E :G= K>EB:;E> BG?HKF:MBHG ?HK E:K@> L<:E> :=:IM:
MBHG H? - 1A> D>R BLLN>L G>>=>= MH ;> :==K>LL>= BG LN<A L<:E>NI LMN=
B>L F:R BG<EN=> K>:<MHK =>LB@G ;BHF:LL LNIIER <A:BG =><HGM:FBG:MBHG
:G= <HHEBG@ H? ?>>=LMH<DL IK>I:K:MBHG H? BGH<NENF HK >GSRF> <H<DM:BEL
FHGBMHKBG@ H? FB<KH;B:E @KHPMA :G= F>M:;HEBLF M>FI>K:MNK> :G= O>G
MBE:MBHG <HGMKHE :G= >O:EN:MBHG H? MA> ><HGHFB< ?:<MHKL ?HK <HLM >LMBF:
MBHG 89
,G> H? MA> FHLM IKHFBLBG@ ?NMNK> K>L>:K<A MK>G=L BG - PHNE=
;> MA> =>O>EHIF>GM H? IHM>GM ;BH:@>GM LRLM>FL PAB<A F:R BG<EN=>
>BMA>K L<K>>GBG@ H? G:MNK:E FB<KHjHK: HK BFIKHO>F>GM H? LMK:BGL NL
BG@ O:KBHNL K:MBHG:E :G= :=O:G<>= LMK:BGBFIKHO>F>GM M><AGHEH@B>L &G
MABL K>@:K= KNFBG:GM FB<KHjHK: <HNE= @>M K>L>:K<A ?H<NL>L ?HK H;M:BG
BG@ MA> IHM>GM AR=KHERMB< LMK:BGL /NF>G FB<KHHK@:GBLFL MA:M K>LB=>
BG MA> :EBF>GM:KR <:G:E H? KNFBG:GM :GBF:EL AR=KHERS> O:KBHNL K>
<:E<BMK:GM <HFIHG>GML IK>L>GM BG MA> IE:GM <>EE P:EEL ?HK A:OBG@ MA>BK
:;BEBMR MH IKH=N<> :M E>:LM  >QMK:<>EENE:K AR=KHERMB< >GSRF>L 89
?M>K : K>OB>P HG MA> :<MBOBMB>L :G= IKHLI><ML H? KNF>G FB<KHjHK:
:KKBEEH/>R>L >M :E 89 A:O> <HG<EN=>= MA:M MA>L> FB<KHHK@:GBLFL
<:G AHE= FN<A IHM>GMB:E ?HK IK>MK>:MF>GM H? FB<KH:E@:> 1ABL A:L ?NK
MA>K ;>>G IKHO>G ;R : ?>P K><>GM LMN=B>L MA:M K>IHKM>= MA> IKHFBL
BG@ >QI>KBF>GM:E K>LNEML HG - H? FB<KH:E@:> ;R KNF>G FB<KHHK@:G
BLFL 89 %HP>O>K FHK> K>L>:K<A >??HKML LAHNE= ;> =>OHM>=
HG MA> @>G>MB<L :G= F><A:GBLFL I:MAP:RL ?HK MA> AR=KHERMB< :<MBO
BMB>L H? KNFBG:GM LMK:BGL &G MABL K>@:K= ?NKMA>K >??HKML LAHNE= ;> ?H
<NL>= HG :<AB>OBG@ MA> ;>MM>K >?i<B>G<R LI><Bi<BMR :G= MHE>K:G<> ;R :I
IERBG@ F>M:;HEB< >G@BG>>KBG@ 89 FNM:@>G>LBL :G= @>GHF> LAN?jBG@
89 0>O>K:E FH=>KG :G= :=O:G<>= M><AGHEH@B>L :K> GHP :O:BE:;E>
?HK @>G> >=BMBG@ MA:M <:G ;> :IIEB>= BG MA> EB@GBGHERMB< :G= AR=KHERMB<
FB<KHHK@:GBLFL ?HK BG<K>:LBG@ F>M:;HEB< :G= IK>MK>:MF>GM >?i<B>G<R
1A> FH=>KG @>G> >=BMBG@ MHHEL :K> <ENLM>K>= K>@NE:KER BGM>KLI:<>=
LAHKM I:EBG=KHFB< K>I>:ML  /&0-/ PBMA :LLH<B:M>= GN<E>:L>L  :L
SBG< iG@>K IKHM>BGL 7+#L :G= MK:GL<KBIMBHG :<MBO:MHKEBD> >??><MHK GN
<E>:L>L 1)"+L 89
1H :<AB>O> M><AGH><HGHFB< OB:;BEBMR BG :GR ;BH<HGO>KLBHG IKH<>LL
BGM>@K:M>= ;BHK>iG>KR BL GHP:=:RL PB=>ER <HGLB=>K>= :L : IKHFBLBG@
<HG<>IM MA:M @>G>K:M>L ;BH?N>EL :G= ;BHIKH=N<ML BG MA> L:F> IE:M?HKF
<<HK=BG@ MH L>O>K:E K>IHKML BGM>@K:MBHG H? $ $ :G= $ ;BH?N>EL PBMA
HMA>K ;BHIKH=N<M LRGMA>LBL <:G IKHOB=> LN;LM:GMB:E M><AGH><HGHFB< OB
:;BEBMR BG MA> HO>K:EE IKH<>LL 89 %HP>O>K :IIEB<:MBHG H? - BG
;BHIKH=N<M LRGMA>LBL ?KHF ;BHF:LL K>F:BGL :EFHLM HNM H? MA> K>L>:K<A
:K>: PABE> - >LI><B:EER >GSRF:MB< IK>MK>:MF>GM H? ;BHF:LL A:L MA>
IHM>GMB:E MH ;> NL>= BG FB<KH:E@:>;:L>= ;BHK>iG>KB>L 89 &G K><>GM
R>:KL ;BHF:LL =>KBO>= LN@:KL :G= ;BH?N>E =>KBO>= @ER<>KHE :K> PB=>ER
NL>= ?HK ;BHF:GN?:<MNKBG@ H? O:KBHNL IE:M?HKF <A>FB<:EL BG ;BHK>iG
>KR PAB<A BG<EN=> -KHI:G>=BHE 89 !M:@:MHL> 89 !:K:;BMHE
89 %R=KHQRIKHIBHGB< :<B= 89 QREBMHE 89 :G= LH HG &F
IKHO>F>GM :G= :IIEB<:MBHG H? - BG ;BHK>iG>KR PBEE E:K@>ER >QM>G= MA>
:IIEB<:MBHG H? MABL IK>MK>:MF>GM M><AGBJN> PAB<A BG MNKG PBEE IKHOB=>
M><AGH><HGHFB< OB:;BEBMR BG MA> ;BH?N>E IKH=N<MBHG NLBG@ -
HGLHEB=:M>= ;BHIKH<>LLBG@  - BL :G >F>K@BG@ M><AGHEH@B<:E
IKH@K>LL BG MA> @>G>K:MBHG H? ;BH?N>EL PAB<A FHLMER ?H<NL>L HG MA> BGM>
@K:MBHG HK FBGBFBS:MBHG H? MA> HO>K:EE IKH<>LL LM>IL L F>GMBHG>= >:K
EB>K 0><MBHG  ;BH<HGO>KLBHG H? ;BHF:LL BGMH ;BH?N>EL K>JNBK>L LHF>
@>G>K:E LM>IL BG<EN=BG@ IK>MK>:MF>GM AR=KHERLBL :G= ?>KF>GM:MBHG MA:M
F:D> MA> ;BH<HGO>KLBHG IKH<>LL <HFIEB<:M>= B? <HFI:K>= PBMA MA> IKH
=N<MBHG H? $ ;BH?N>EL 1H FBGBFBS> MA> LM>IL L>O>K:E <HF;BG:MBHGL
A:O> :EK>:=R ;>>G K>IHKM>= #HK >Q:FIE> : L>I:K:M> L:<<A:KBi<:MBHG
LM>I <:G ;> FBGBFBS>= ;R :IIERBG@ LBFNEM:G>HNL L:<<A:KBi<:MBHG :G=
?>KF>GM:MBHG 00# 89 &G MA>L> BGM>@K:MBHG :IIKH:<A>L IK>MK>:MF>GM
A:L ;>>G B@GHK>= :G= MANL : L>I:K:M> IK>MK>:MF>GM LM>I BL LMBEE K>JNBK>=
- <:G IKHOB=> : HG>LM>I IKH<>LL ?HK <HGO>KMBG@ ;BHF:LL BGMH ;BH?N
>EL PA>K> FB<KHHK@:GBLFL NL>= BG - <HNE= ;> MA> FHLM IKHFBLBG@ ;BH:
@>GML ?HK =>O>EHIBG@ : LN<<>LL?NE - 1H =>O>EHI - @>G>MB< >G@B
G>>KBG@ BL NLN:EER K>JNBK>= MH >LM:;EBLA MA> >GSRF> LRLM>FL HK I:MAP:RL
BG MA> LMK:BG LH MA:M : LBG@E> FB<KHHK@:GBLF <:G I>K?HKF :EE MA> G><>L
L:KR <HGO>KLBHGL 89 2MBEBS:MBHG H? LMK:BGL BG - PBMA MA> <:
I:;BEBMR H? IK>MK>:MBG@ ;BHF:LL PHNE= IKHOB=> :G >:LR LMK:M>@R ?HK >L
M:;EBLABG@ - LMK:BG #HK >Q:FIE> ?HNK >G=HIARMB< ?NG@:E LMK:BGL P>K>
K><>GMER K>IHKM>= MH A:O> K>L>KOHBKL H? AR=KHERMB< :G= EB@GBGHERMB< >G
SRF>L PAB<A P>K> F:KD>= :L MA> IKHFBLBG@ ?>:MNK>L H? MA>L> LMK:BGL ?HK
=>O>EHIBG@ - 89
8. Conclusion
HFI:K>= MH MA> <HGO>GMBHG:E MA>KFH<A>FB<:E IK>MK>:MF>GM M><A
GBJN>L - <HNE= ;> FHK> IKHFBLBG@ ?HK ;BHF:LL IK>MK>:MF>GM =N> MH
BML EHP>K >G>K@R <HGLNFIMBHG K>JNBK>F>GM FBE=>K HI>K:MBG@ <HG=BMBHGL
:G= EHP>K ;RIKH=N<M ?HKF:MBHG %HP>O>K <HFF>K<B:E >QIEHBM:MBHG H?
MABL M><AGBJN> BL LMBEE BG BML BG?:G<R ?HK >G<HNGM>KBG@ LHF> ANK=E>L &G
I:KMB<NE:K - H? )  BL ABG=>K>= ;R EHG@ BG<N;:MBHG MBF> AB@A <:K;HAR
=K:M> EHLL :G= EHP >?i<B>G<R )BD>PBL> O:KB:MBHGL BG MA> K><:E<BMK:G<>
:G= <HFIHLBMBHG H? MA> <>EE P:EEL :FHG@ MA> FB<KH:E@:E LI><B>L :K> F:
CHK ABG=K:G<>L BG MA> - H? FB<KH:E@:> +>O>KMA>E>LL : ?NKMA>K <HFIK>
A>GLBO> :G= K>:EPHKE= M><AGH><HGHFB< K>L>:K<A >??HKML <HNE= HO>K<HF>
MA>L> BLLN>L :G= IKHOB=> : LNLM:BG:;E> - ?HK E:K@> L<:E> IK:<MB<>L E
MAHN@A : ?>P MA>KFH<A>FB<:E IK>MK>:MF>GM F>MAH=L A:O> LAHPG : ;>M
M>K I>K?HKF:G<> MA:G - :G= A:O> ;>>G :=:IM>= BG E:K@>L<:E> ?:<BEBMB>L
MA>L> :IIKH:<A>L :K> GHM ><HGHFB< :G= ><H?KB>G=ER 0H - BL GHP BG
<K>:LBG@ER @:BGBG@ @K>:M>K :MM>GMBHG :G= BM PHNE= K>:EER ;> GB<> MH iG=
- :L : LM:G=:EHG> :G= LNLM:BG:;E> IK>MK>:MF>GM F>MAH= BG MA> G>:K ?N
MNK>

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
Acknowledgements
1ABL PHKD P:L LNIIHKM>= ;R MA> +:MBHG:E +:MNK:E 0<B>G<> #HNG=:
MBHG H? ABG: $K:GM +H  ABG: -HLM=H<MHK:E 0<B>G<> #HNG
=:MBHG $K:GM +H * %B@AE>O>E M:E>GML IKHC><M H? 0BQ 1:E
>GM ->:DL -KHC><M BG 'B:G@LN -KHOBG<> $K:GM +H 0466 :G= +:
MBHG:E (>R / ! -KH@K:F $K:GM +H 6# 
Declarations of interest
+HG>
References
89   0:A: + .NK>LAB $' (>GG>=R * HMM: BHEH@B<:E IK>MK>:MF>GM H? <HKG
LMHO>K PBMA PABM>KHM ?NG@NL ?HK BFIKHO>= >GSRF:MB< AR=KHERLBL &GM BH=>M>KBHK
BH=>@K:=   
89 ' 3:L<H HKK>: 5 $> 6 )B BHEH@B<:E IK>MK>:MF>GM H? EB@GH<>EENEHLB< ;BHF:LL
BG 0& *NLL:MMH "= BHF:LL ?K:<MBHG:MBHG M><AGHEH@B>L ?HK : EB@GH<>EENEHLB<
?>>=LMH<D ;:L>= ;BHK>iG>KR "EL>OB>K FLM>K=:F  II 
89  =: 0BEO: *:<A:=H  #>KK:S BHEH@B<:E IK>MK>:MF>GM H? LN@:K<:G> ;:@:LL>
PBMA ;:LB=BHFR<>M>L IKH=N<BG@ O:KB>= I:MM>KGL H? ;BH=>@K:=:MBHG BHK>LHNK
1><AGHE   
89 / 0BG=AN - BGH=  -:G=>R BHEH@B<:E IK>MK>:MF>GM H? EB@GH<>EENEHLB< ;BH
F:LLG HO>KOB>P BHK>LHNK 1><AGHE   
89 / *BEE:MB 0 0R:FLB:A +BDE:LLHG *+ :AR:GMH ( )N=JNBLM *'
1:A>KS:=>A BHEH@B<:E IK>MK>:MF>GM H? EB@GH<>EENEHL>L PBMA PABM>KHM ?NG@B :G=
BML :IIEB<:MBHGL : K>OB>P BH/>LHNK<>L   
89 / KHK: +( 0A:KF: 0 (NF:K 3:EHKBS:MBHG H? ;RIKH=N<ML ?HEEHPBG@ MA>
;BHK>iG>KR <HG<>IM <HFF>K<B:E :LI><ML H? ;RIKH=N<ML H? EB@GH<>EENEHLB< ;BH
F:LL BG ( A:G=>E *% )N<B:GH 0BEO>BK: "=L =O:G<>L BG LN@:K<:G>
;BHK>iG>KR "EL>OB>K FLM>K=:F  II 
89 ' 0BG@A 0 $N HFF>K<B:EBS:MBHG IHM>GMB:E H? FB<KH:E@:> ?HK ;BH?N>EL IKH=N<
MBHG />G>P 0NLM:BG "G>K@R />O   
89 " AF:= (( -:GM )B@GBG <HGO>KLBHG : D>R MH MA> <HG<>IM H? EB@GH<>EENEHLB<
;BHF:LL;:L>= BGM>@K:M>= ;BHK>iG>KR BG 1 A:LD:K  -:G=>R 03 *HA:G
!' )>> 0( (A:G:E "=L 4:LM> ;BHK>iG>KR "EL>OB>K FLM>K=:F  II

89 % 7:;>= 0 0NEM:G: '+ 0:AN 5 .B G HO>KOB>P HG MA> :IIEB<:MBHG H? EB@GB
GHERMB< FB<KHHK@:GBLFL :G= >GSRF>L ?HK IK>MK>:MF>GM H? EB@GH<>EENEHLB< ;BHF:LL
BG -( 0:K:G@B 0 +:G=: - *HA:GMR "=L /><>GM :=O:G<>F>GML BG ;BH?N>EL
:G= ;BH>G>K@R NMBEBS:MBHG 0IKBG@>K+:MNK> 0BG@:IHK>  II 
89 ' :KKBEEH/>R>L * :KK:@TG1KBGB=:= $ NBMK\G BHEH@B<:E IK>MK>:MF>GML H?
FB<KH:E@:E ;BHF:LL ?HK @:L>HNL ;BH?N>E IKH=N<MBHG :G= MA> IHM>GMB:E NL> H? KN
F>G FB<KHHK@:GBLFL : K>OB>P E@:E />L   
89 5 7A:H ( )NH 6 7A:G@ 7 7A>G@ 6 :B  4>G >M :E &FIKHOBG@ MA>
F>MA:G> RB>E= H? F:BS> LMK:P ?H<NL HG MA> >??><ML H? IK>MK>:MF>GM PBMA ?NG@B :G=
MA>BK L><K>M>= >GSRF>L <HF;BG>= PBMA LH=BNF AR=KHQB=> BHK>LHNK 1><AGHE
  
89 /$ 0:K:M:E> $ (NF:K / :GN  5B: 0 ->KBR:L:FR $! 0:K:M:E>  <KBMB<:E
K>OB>P HG :G:>KH;B< =B@>LMBHG H? FB<KH:E@:> :G= F:<KH:E@:> :G= <H=B@>LMBHG H?
;BHF:LL ?HK >GA:G<>= F>MA:G> @>G>K:MBHG BHK>LHNK 1><AGHE  

89 ' 3:L<H HKK>: 5 $> 6 )B #NG@:E IK>MK>:MF>GM H? GHGLM>KBE> FBL<:GMANL ?HK
>GA:G<>= >GSRF:MB< AR=KHERLBL BHK>LHNK 1><AGHE   
89 ! %>KGTG=>S  /B:[H * H<: *  $:K<Z:$HGSTE>S 0:<<A:KBi<:MBHG H? <:K
;HAR=K:M>L BG FB<KH:E@:E ;BHF:LL ;R IARLB<:E <A>FB<:E :G= >GSRF:MB< IK>MK>:M
F>GML :L : IK>OBHNL LM>I ?HK ;BH>MA:GHE IKH=N<MBHG A>F "G@ '  

89 / %:KNG *( !:GJN:A "GSRF:MB< AR=KHERLBL H? FB<KH:E@:E ;BHF:LL ?HK
;BH>MA:GHE IKH=N<MBHG A>F "G@ '   
89 # -:LLHL " 2@@>MMB % :KKXK> & #>KK>K -K>MK>:MF>GM H? FB<KH:E@:> MH BFIKHO>
;BH@:L IKH=N<MBHG : K>OB>P BHK>LHNK 1><AGHE   
89 3 :KN: 33 $HN= 0 (:E:F=A:= *B<KH;B:E IK>MK>:MF>GM H? P:M>K AR
:<BGMA ?HK >GA:G<>= AR=KHERLBL ?HEEHP>= ;R ;BH@:L IKH=N<MBHG />G>P "G>K@
  
89 -0 +B@:F  0BG@A -KH=N<MBHG H? EBJNB= ;BH?N>EL ?KHF K>G>P:;E> K>LHNK<>L
-KH@ "G>K@R HF;NLM 0<B   
89  !>FBK;:L -HEBMB<:E ><HGHFB< :G= >GOBKHGF>GM:E BFI:<ML H? ;BH?N>EL : K>
OB>P IIE "G>K@   00
89 % 7:;>= $ #:KNJ + HR<> '+ 0:AN - $:G>L:G "O:EN:MBHG H? AB@A LN@:K
<HGM:BGBG@ <HKG @>GHMRI>L :L OB:;E> ?>>=LMH<DL ?HK =><K>:LBG@ >GSRF> <HGLNFI
MBHG =NKBG@ =KR@KBG= >MA:GHE IKH=N<MBHG ' 1:BP:G &GLM A>F "G@  

89 % 7:;>= + HR<> ' 0:AN $ #:KNJ "O:EN:MBHG H? MA> JN:EBMR H? =KB>= =BL
MBEE>KL @K:BGL PBMA LHEN;E>L ?HK GHKF:E :G= AB@A LN@:KR <HKG @>GHMRI>L =NKBG@
=KR@KBG= >MA:GHE IKH=N<MBHG ' E>:G -KH=   
89 *# !>FBK;:L BHK>iG>KB>L ?HK ;BH?N>E NI@K:=BG@ : <KBMB<:E K>OB>P IIE "G>K@
  00
89 % 7:;>= $ #:KNJ '+ 0:AN *0 SBKNG / %:LABF  +:LKNEA:J HR<>
BH>MA:GHE IKH=N<MBHG ?KHF ?>KF>GM:;E> LN@:K CNB<> 0<B 4HKE= '  AMMIL
=HBHK@
89 % 7:;>= '+ 0:AN  0N>ER + HR<> $ #:KNJ BH>MA:GHE IKH=N<MBHG ?KHF
K>G>P:;E> LHNK<>L <NKK>GM I>KLI><MBO>L :G= M><AGHEH@B<:E IKH@K>LL />G>P 0NL
M:BG "G>K@R />O   
89 % 7:;>= $ #:KNJ ' 0:AN  HR<> - $:G>L:G  <HFI:K:MBO> LMN=R HG GHK
F:E :G= AB@A LN@:KR <HKG @>GHMRI>L ?HK >O:EN:MBG@ >GSRF> <HGLNFIMBHG =NKBG@
=KR@KBG= >MA:GHE IKH=N<MBHG A>F "G@ '   
89 d) 0:GM:G: * *>BK>E>L +>P LM:K<A>L :K> MA> MK>G= ?HK BG=NLMKR :IIEB<:
MBHGL : K>OB>P #HH= -N;EB< %>:EMA   
89 % 7:;>=  HR<> $ #:KNJ ' 0:AN  <HFI:K:MBO> >O:EN:MBHG H? :@KHGHFB<
I>K?HKF:G<> :G= D>KG>E <HFIHLBMBHG H? GHKF:E :G= AB@A LN@:KR <HKG @>GHMRI>L
7>: F:RL ) @KHPG ?HK =KR@KBG= >MA:GHE IKH=N<MBHG &G= KHI -KH=  

89 + $:NK:O 0 0BO:L:GD:KB $0 (BK:G  +BG:P> ' 0>EOBG 2MBEBS:MBHG H? ;BHK>
LHNK<>L ?HK LNLM:BG:;E> ;BH?N>EL : K>OB>P />G>P 0NLM:BG "G>K@R />O  

89 * /HL>G "GOBKHGF>GM:E LNLM:BG:;BEBMR MHHEL BG MA> ;BH?N>E BG=NLMKR BH?N>E
/>L '   
89 # A>KN;BGB +! BK=  HPB> $ 'NG@F>B>K  0<AE:F:=BG@>K 0 4H>LL$:E
E:L<A "G>K@R:G= @K>>GAHNL> @:L;:L>= )  H? ;BH?N>E :G= ;BH>G>K@R LRLM>FL
D>R BLLN>L K:G@>L :G= K><HFF>G=:MBHGL />LHNK HGL>KO /><R<E  

89 ( !NMM:  !:O>K>R '$ )BG "OHENMBHG K>MKHLI><MBO> ?HK :EM>KG:MBO> ?N>EL iKLM
MH ?HNKMA @>G>K:MBHG />G>P "G>K@   
89 0 <ABG:L $'4 "NO>KBGD HGLHEB=:M>= ;KB>?BG@ H? ;BH<A>FB<:E >MA:GHE IKH
=N<MBHG ?KHF EB@GH<>EENEHLB< ;BHF:LL "E><MKHG ' BHM><AGHE   
89 1!% N@@ * AF:= "* %:K=BF:G / /:AF:GIHNK -:MAP:RL ?HK =>@K:=:
MBHG H? EB@GBG BG ;:<M>KB: :G= ?NG@B +:M -KH= />I   
89  )BF:R>F 0  /B<D> )B@GH<>EENEHLB< ;BHF:LL ?HK ;BH>MA:GHE IKH=N<MBHG <NK
K>GM I>KLI><MBO>L IHM>GMB:E BLLN>L :G= ?NMNK> IKHLI><ML -KH@ "G>K@R HF;NLM 0<B
  
89 $ => $HGS:EH !& HEI: *% %:;B; *4 #K::BC> :<M>KB:E >GSRF>L BGOHEO>=
BG EB@GBG =>@K:=:MBHG ' BHM><AGHE   
89 ) 7A:G@ $ %>GKBDLLHG $ $>EE>KLM>=M 1A> ?HKF:MBHG H? β–β LMKN<MNK>L BG EB@GBG
;BHLRGMA>LBL:K> MA>K> MPH =B??>K>GM I:MAP:RL ,K@ BHFHE A>F 

89 / EF:KL=\MMBK & $NGG:KLLHG #K:FE>BhLE: >M:G\EL ^K j\DGNF EZ?F:LL: F>h
ABM:DcKNF ;:DM>KZNF 2GBO>KLBMR H? DNK>RKB  AMMILLD>FF:GBLA:G=E>

89 ' -WK>S ' *NGHS!HK:=H 1 => E: /N;B: ' *:KMBG>S BH=>@K:=:MBHG :G= ;BH
EH@B<:E MK>:MF>GML H? <>EENEHL> A>FB<>EENEHL> :G= EB@GBG :G HO>KOB>P &GM *B<KH
;BHE   
89 0( -K:C:I:MB - (:NLABD  *:EBD 3( 3BC:R -AR<HK>F>=B:MBHG <HNIE>= IKH
=N<MBHG H? :E@:E ;BHF:LL A:KO>LMBG@ :G= :G:>KH;B< =B@>LMBHG IHLLB;BEBMB>L :G=
<A:EE>G@>L BHM><AGHE =O   
89 $HGSTE>S#>KGTG=>S  0B:EO> + >KG>M '- 0M>R>K &FI:<M H? FB<KH:E@:>
<A:K:<M>KBLMB<L HG MA>BK <HGO>KLBHG MH ;BH?N>E -:KM && ?H<NL HG ;BHF>MA:G> IKH
=N<MBHG BH?N>E BHIKH= BHK>iG   
89 ' 3>E:SJN>S)N<BH /* /H=KZ@N>S':LLH )* HEE:  0T>GS$:EBG=H !" >K
O:GM>L BLG>KHL + @NBE:K >M :E *B<KH:E@:E ;BHF:LL IK>MK>:MF>GM ?HK
;BH>MA:GHE IKH=N<MBHG : K>OB>P BH?N>E />L '   
89 " 2@@>MMB  0B:EO> " 1K:;ER '- 0M>R>K &GM>@K:MBG@ FB<KH:E@:> IKH=N<MBHG
PBMA :G:>KH;B< =B@>LMBHG : ;BHK>iG>KR :IIKH:<A BH?N>E BHIKH= BHK>iG
  
89 6 A>G 5. 7A:H %4 6>G 0% %H ) A>G@ !' )>> >M :E *B
<KH:E@:>;:L>= <:K;HAR=K:M>L ?HK ;BH?N>E IKH=N<MBHG BH<A>F "G@ '  

89 0BF:L/H=KB@N>L %!* 3BEE>E: - *:KMBGL )$ *:KJN>L - HE>IB<HEH
- 1HGHG *B<KH:E@:> ?HK ><HGHFB< :IIEB<:MBHGL :=O:GM:@>L :G= I>KLI><MBO>L
?HK ;BH>MA:GHE ' "QI HM   
89 7 -HII>K *$ 1NHAR >RHG= MA> @K>>G NG=>KLM:G=BG@ MA> >OHENMBHG:KR INS
SE> H? IE:GM :G= :E@:E <>EE P:EEL -E:GM -ARLBHE   
89 *' 0<AHES 1) 4>BLL /" 'BGD>KLHG ' 'BG@ / /HMA 2 $HH=>GHN@A >M :E
2EMK:LMKN<MNK> :G= <HFIHLBMBHG H? MA> +:GGH<AEHKHILBL @:=BM:G: <>EE P:EE "N
D:KRHM >EE   
89 0 +:G=: ' *HA:FF:= 0+ />==R ' (HSBGLDB ( !:E:B -:MAP:RL H? EB@
GH<>EENEHLB< ;BHF:LL <HGO>KLBHG MH K>G>P:;E> ?N>EL BHF:LL HGO>KL BHK>iG
  
89 ( 0DCUG>L - )BG=;E:= ' *NEE>K BH , : FNEMB=BL<BIEBG:KR ;BHEH@B<:E :I
IKH:<A NLBG@ LHE:K >G>K@R MH <:IMNK> , PABE> IKH=N<BG@ % :G= AB@A O:EN>
IKH=N<ML BHFHE "G@   
89 7 0A>G ( 7A:G@ * 0B * )BN 0 7ANH ! )BN >M :E 0RG>K@R H? EB@GH<>EENEHL>L
IK>MK>:MF>GM ;R LH=BNF <:K;HG:M> :G= ;:<M>KBNF MH >GA:G<> >GSRF:MB< AR=KHER
LBL H? KB<> LMK:P BHK>LHNK 1><AGHE   
89 00 %:LL:G $ 4BEEB:FL ( ':BLP:E "F>K@BG@ M><AGHEH@B>L ?HK MA> IK>MK>:M
F>GM H? EB@GH<>EENEHLB< ;BHF:LL BHK>LHNK 1><AGHE   
89 14* %>G=KBDL $ 7>>F:G -K>MK>:MF>GML MH >GA:G<> MA> =B@>LMB;BEBMR H? EB@
GH<>EENEHLB< ;BHF:LL BHK>LHNK 1><AGHE   

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
89 % 7:;>= ' 0:AN  HR<> $ #:KNJ #N>E >MA:GHE IKH=N<MBHG ?KHF EB@GH<>EEN
EHLB< ;BHF:LL :G HO>KOB>P HG ?>>=LMH<DL :G= M><AGHEH@B<:E :IIKH:<A>L />G>P
0NLM:BG "G>K@R />O   
89 # -:LLHL ' :KK>M>KH & #>KK>K HFI:KBG@ IK>MK>:MF>GM F>MAH=L ?HK BFIKHOBG@
FB<KH:E@:> :G:>KH;B< =B@>LMBHG MA>KF:E AR=KHMA>KF:E FB<KHP:O> :G= NEMK:
LHNG= A>F "G@ '   
89 ! (NF:KB / 0BG@A -K>MK>:MF>GM H? EB@GH<>EENEHLB< P:LM>L ?HK ;BH?N>E IKH=N<
MBHG : <KBMB<:E K>OB>P />G>P 0NLM:BG "G>K@R />O   
89  $NIM: '- 3>KF: 0NLM:BG:;E> ;BH>MA:GHE IKH=N<MBHG ?KHF :@KHK>LB=N>L :
K>OB>P />G>P 0NLM:BG "G>K@R />O   
89   0:A: $' (>GG>=R + .NK>LAB * HMM: BHEH@B<:E IK>MK>:MF>GM H? <HKG
LMHO>K PBMA -AE>;B: ;K>OBLIHK: +//) ?HK >GA:G<>= >GSRF:MB< AR=KHERLBL
:G= >?i<B>GM >MA:GHE IKH=N<MBHG BHM><AGHE -KH@   
89 6 7A>G@ ' 7A:H # 5N 6 )B -K>MK>:MF>GM H? EB@GH<>EENEHLB< ;BHF:LL ?HK >G
A:G<>= ;BH@:L IKH=N<MBHG -KH@ "G>K@R HF;NLM 0<B   
89 4 ANMMH ( .NK>LAB ( %:KBC:G / ;KH 1 ;;:L  :SFB >M :E &GLB@AM
BGMH IKH@K>LL BG IK>MK>:MF>GM H? EB@GH<>EENEHLB< ;BHF:LL "G>K@R  

89 3 *BLAK: ( ':G: ** ':G:  $NIM: "GA:G<>F>GM BG FNEMBIE> EB@GHERMB<
>GSRF>L IKH=N<MBHG ?HK HIMBFBS>= EB@GBG =>@K:=:MBHG :G= L>E><MBOBMR BG ?NG@:E
IK>MK>:MF>GM H? LP>>M LHK@ANF ;:@:LL> BHK>LHNK 1><AGHE   
89 + 6:AF>= % :KK>K> *+ *:KSHNDB & 0F::EB "GA:G<>F>GM H? ;BH@:L IKH
=N<MBHG ?KHF 2EO: LI ;R NLBG@ LHEB=LM:M> ?>KF>GM:MBHG :L ;BHEH@B<:E IK>MK>:M
F>GM E@:E />L   
89 ) 6:G@ # 5N 5 $> 6 )B A:EE>G@>L :G= LMK:M>@B>L ?HK LHEB=LM:M> :G:>KH;B< =B
@>LMBHG H? EB@GH<>EENEHLB< ;BHF:LL />G>P 0NLM:BG "G>K@R />O  

89 4:G 6 )B #NG@:E IK>MK>:MF>GM H? EB@GH<>EENEHLB< ;BHF:LL BHM><AGHE =O
  
89 " /HN<A>L & %>KIHYE$BF;>KM ' 0M>R>K % :KK>K> &FIKHO>F>GM H? :G:>KH;B<
=>@K:=:MBHG ;R PABM>KHM ?NG@B IK>MK>:MF>GM H? EB@GH<>EENEHLB< ;BHF:LL : K>OB>P
/>G>P 0NLM:BG "G>K@R />O   
89 5 6:G 7 4:G@ ( 7A:G@ * 0B * )BN ) A:B >M :E :<M>KB:>GA:G<>= =BENM>
:<B= IK>MK>:MF>GM H? EB@GH<>EENEHLB< ;BHF:LL BHK>LHNK 1><AGHE  

89 6 0AB 5 6:G . )B 5 4:G@ * EBN 0 5B> >M :E !BK><M>= ;BH<HGO>KLBHG H?
(K:?M EB@GBG MH IHERAR=KHQR:ED:GH:M> ;R NIKB:OB=NL ;:LBE>GLBL  PBMAHNM :GR
IK>MK>:MF>GM -KH<>LL BH<A>F   
89 1!% N@@ * AF:= "* %:K=BF:G / 0BG@A 1A> >F>K@BG@ KHE> ?HK ;:<M>
KB: BG EB@GBG =>@K:=:MBHG :G= ;BHIKH=N<M ?HKF:MBHG NKK ,IBG BHM><AGHE
  
89 ( -HLSRM>D * B>SDHPLD:  0DEH=HPLD: ) !K>PGB:D *B<KH;B:E <HGLHKMBNF
PBMA AB@A <>EENEHERMB< :<MBOBMR * %  ?HK >GA:G<>= ;BH@:L IKH=N<MBHG #KHGM
*B<KH;BHE    AMMIL=HBHK@?FB<;
89 0 4HG@PBE:BP:EBG 2 /:MM:G:<AHFLKB 1 ):HMA:G:<A:K>HG ) "NKPBE:B<ABMK 6
&@:K:LAB 3 A:FIK>=: G:ERLBL H? : MA>KFHIABEB< EB@GH<>EENEHL> =>@K:=BG@ FB
<KH;B:E <HGLHKMBNF :G= FNEMBLI><B>L EB@GH<>EENEHERMB< >GSRF> LRLM>F "GSRF *B
<KH; 1><AGHE   
89 . 7A:G@ ' %> * 1B:G 7 *:H ) 1:G@ ' 7A:G@ >M :E "GA:G<>F>GM H?
F>MA:G> IKH=N<MBHG ?KHF <:LL:O: K>LB=N>L ;R ;BHEH@B<:E IK>MK>:MF>GM NLBG@ :
<HGLMKN<M>= FB<KH;B:E <HGLHKMBNF BHK>LHNK 1><AGHE   
89  %HF!B:S # -:LLHL & #>KK>K 1 3B<>GM - ETGJN>S "GSRF:MB< IK>MK>:MF>GM
H? FB<KH:E@:> NLBG@ ?NG@:E ;KHMA ?KHF 1K:F>M>L O>KLB<HEHK :G= <HFF>K<B:E E:<
<:L> ?HK BFIKHO>= ;BH@:L IKH=N<MBHG E@:E />L   
89 0 A:N=KR - :AKB +/ *HA>BF:GB 1><AGH><HGHFB< :G:ERLBL H? FBEDBG@ H?
HMKRH<H<<NL ;K:NGBB ?HK K>G>P:;E> AR=KH<:K;HG IKH=N<MBHG E@:E />L
  
89 ( -AP:G %  ,G@ 4% A>G 1  )BG@ "- +@ -) 0AHP ,O>KOB>P
<HFI:KBLHG H? IK>MK>:MF>GM M><AGHEH@B>L :G= ?>KF>GM:MBHG IKH<>LL>L H?
;BH>MA:GHE ?KHF FB<KH:E@:> "G>K@ HGO>KL *:G:@   
89 * HKHPBMSD: +/ *HA>BF:GB 0NLM:BG:;E> ;BH?N>EL ?KHF :E@:> *BMB@ =:IM
0MK:M $E   
89 6 )B * %HKLF:G + 4N . ):G + !N;HBL :E>KH BH?N>EL ?KHF FB<KH:E@:>
BHM><AGHE -KH@   
89 ( (NF:K 0 $AHLA & G@>EB=:DB 0) %HE=M ! (:K:D:LA>O * *HK:E>L >M
:E /><>GM =>O>EHIF>GML HG ;BH?N>EL IKH=N<MBHG ?KHF FB<KH:E@:> :G= F:<KH:E
@:> />G>P 0NLM:BG "G>K@R />O   
89 1 &LABD: +/ *HA>BF:GB - :AKB 0NLM:BG:;E> L:EBG> FB<KH:E@:> <H<NEMBO:
MBHG ?HK ;BH?N>E IKH=N<MBHG : <KBMB<:E K>OB>P />G>P 0NLM:BG "G>K@R />O
  
89 1 &LABD: - :AKB !4 ):BK= +/ *HA>BF:GB 1A> >??><M H? @K:=N:E BG<K>:L>
BG L:EBGBMR HG MA> ;BHF:LL IKH=N<MBOBMR :G= ;BH<A>FB<:E <HFIHLBMBHG H? L>O>K:E
F:KBG> A:EHMHE>K:GM :G= A:EHIABEB< FB<KH:E@:> ' IIE -AR<HE  

89 1 &LABD: +/ *HA>BF:GB - :AKB !4 ):BK= 0 E:BK ! -:KE>OEB>M
%:EH:=:IM>= FB<KH:E@:> ?HK ?N<HQ:GMABG IKH=N<MBHG >??><M H? BG<K>F>GM:E BG
<K>:L> BG L:EBGBMR E@:E />L   
89 BH>G>K@R " HFF>K<B:EL<:E> =>FHGLMK:MBHGL H? :E@:> ?HK ;BH?N>EL IKH=N<MBHG
 AMMIPPP>MBI;BH>G>K@R>NO:EN><A:BGL<HGO>KLBHGM><AGHEH@B>L
:=O:G<>=M><AGHEH@B>L<HGO>KLBHGH?:JN:MB<;BHF:LL
<HFF>K<B:EL<:E>=>FHGLMK:MBHGLH?:E@:>?HK;BH?N>ELIKH=N<MBHG
89 ,BE@:> E@:> ;BH=B>L>E <HFF>K<B:E O>GMNK>L  AMMIPPPHBE@:><HF
:E@:>HBE;BH=<HL<HLAMFE
89 ( %:FF>E #NG@:E =>@K:=:MBHG H? EB@GBG BG $ :=BL<A (" $BEE>K "=L !KBO>G
;R G:MNK> IE:GM EBMM>K JN:EBMR :G= =><HFIHLBMBHG  &GM>KG:MBHG:E 4:EEBG@?HK=
 II 
89 ( A:G=>E   $HGV:EO>L ') 0MK:I 00 =: 0BEO: BH=>EB@GBi<:MBHG H? EB@GH
<>EENEHL> LN;LMK:M>L :G BGMKBGLB< :G= LNLM:BG:;E> IK>MK>:MF>GM LMK:M>@R ?HK <E>:G
>G>K@R IKH=N<MBHG KBM />O BHM><AGHE   
89 " 0ABKD:O:G= 0 :KHNMB:G !' $:I>L / 6HNG@ HF;BG:MBHG H? ?NG@:E :G=
IARLB<H<A>FB<:E IKH<>LL>L ?HK EB@GH<>EENEHLB< ;BHF:LL IK>MK>:MF>GM : K>OB>P
/>G>P 0NLM:BG "G>K@R />O   
89  0 %:LMKNI %HP>EE #% ):KL>G + 0:MABMLNDL:GHA  $HH=>EE ' '>EEBLHG
!B??>K>G<>L BG <KRLM:EEBG> <>EENEHL> FH=Bi<:MBHG =N> MH =>@K:=:MBHG ;R ;KHPG :G=
PABM> KHM ?NG@B #NG@:E BHE   
89 3 K:GM>L ' '>EEBLHG  $HH=>EE -><NEB:KBMB>L H? ;KHPGKHM ?NG@B :G= ;BH<A>FB
<:E #>GMHG K>:<MBHG PBMA K>@:K= MH MA>BK IHM>GMB:E :L : FH=>E ?HK ;BHIKH<>LLBG@
;BHF:LL IIE *B<KH;BHE BHM><AGHE   
89 7 $:H 1 *HKB / (HG=H 1A> IK>MK>:MF>GM H? <HKG LMHO>K PBMA $EH>HIAREENF
MK:;>NF (2 ?HK >GSRF:MB< AR=KHERLBL BHM><AGHE BH?N>E   
89 '0 0<ABEEBG@ ' B / E:G<A>MM> 0* !NG<:G 1/ #BEE>R 24 1L<ABKG>K
)B@GH<>EENEHL> FH=Bi<:MBHGL ;R ;KHPG KHM ?NG@B :G= MA>BK >??><ML :L IK>MK>:M
F>GML HG <>EENEHERLBL BHK>LHNK 1><AGHE   
89 *' /:R !' )>:D -! 0I:GN /' *NKIAR KHPG KHM ?NG@:E >:KER LM:@> =><:R
F><A:GBLF :L : ;BHEH@B<:E IK>MK>:MF>GM ?HK LH?MPHH= ;BHF:LL BG ;BH?N>E IKH=N<
MBHG BHF:LL BH>G>K@   
89  KHK: 0 -KBR: - 0A:KF: 0 0A:KF: ) +:BG "O:EN:MBG@ ;BHEH@B<:E IK>MK>:M
F>GM :L : ?>:LB;E> F>MAH=HEH@R ?HK >MA:GHE IKH=N<MBHG ?KHF I:==R LMK:P BH
<:M:E @KB< BHM><AGHE   
89 0TG<A>S )B@GH<>EENEHLB< K>LB=N>L ;BH=>@K:=:MBHG :G= ;BH<HGO>KLBHG ;R ?NG@B
BHM><AGHE =O   
89 ( ,D:FHMH 0 +:K:R:F:  (:MLNH & 0AB@>F:MLN % 6:G:L> BHLRGMA>LBL H?
I:GBL:E=>AR=> ;R MA> PABM>KHM ;:LB=BHFR<>M> -E>NKHMNL HLMK>:MNL ' BHL<B BH
>G@   
89 * 1:GB@N<AB % 0NSNDB ! 4:M:G:;> ( 0:D:B ( %HLABGH 1 1:G:D: "O:EN:
MBHG H? IK>MK>:MF>GM PBMA -E>NKHMNL HLMK>:MNL ?HK >GSRF:MB< AR=KHERLBL H? KB<>
LMK:P ' BHL<B BH>G@   
89  (:F<A:KH>G 3 A:FIK>=: ) "NKPBE:B<ABMK - HHGL:P:G@ 0<K>>GBG@ :G=
HIMBFBS:MBHG H? I:K:F>M>KL :??><MBG@ ?NG@:E IK>MK>:MF>GM H? HBE I:EF >FIMR ?KNBM
;NG<A "# ;R >QI>KBF>GM:E =>LB@G &GM ' />G>P "G>K@R "GOBKHG "G@  

89 5 7A:G@ % 6N % %N:G@ 6 )BN "O:EN:MBHG H? ;BHEH@B<:E IK>MK>:MF>GM PBMA
PABM> KHM ?NG@B ?HK MA> >GSRF:MB< AR=KHERLBL H? ;:F;HH <NEFL &GM BH=>M>KBHK
BH=>@K:=   
89  #>KK:S -:KK: ' #K>>K ' :>S: ' /H=KZ@N>S A:K:<M>KBS:MBHG H? PABM>
SHG>L IKH=N<>= HG -BGNL K:=B:M: PHH= <ABIL ;R $:GH=>KF: :NLMK:E> :G= >KBIH
KBHILBL LN;O>KFBLIHK: 4HKE= ' *B<KH;BHE BHM><AGHE   
89 4:G 6 )B *B<KH;B:E =>EB@GBi<:MBHG H? <HKG LMHO>K ;R >KBIHKBHILBL LN;O>K
FBLIHK: ?HK BFIKHOBG@ <>EENEHL> =B@>LMB;BEBMR "GSRF *B<KH; 1><AGHE  

89 0 (NF:KB ! !:L BHEH@B<:EER IK>MK>:M>= LN@:K<:G> MHI :L : IHM>GMB:E K:P F:M>
KB:E ?HK MA> >GA:G<>F>GM H? @:L>HNL >G>K@R K><HO>KR ;R MPH LM:@> ;BHARMA:G>
IKH<>LL BHK>LHNK 1><AGHE   
89  $N>KK: / *>G=HGV:  #>KK:S *HE><NE:K P>B@AM =BLMKB;NMBHG H? PHH= <HF
IHG>GML >QMK:<M>= ?KHF -BGNL M:>=: ;BHMK>:M>= ;R >KBIHKBHILBL LN;O>KFBLIHK:
"GSRF *B<KH; 1><AGHE   
89 # +:S:KIHNK !( ;=NEE:A + ;=NEE:A / 7:FBKB "O:EN:MBHG H? ;BHEH@B<:E
IK>MK>:MF>GM H? KN;;>KPHH= PBMA PABM> KHM ?NG@B ?HK >GSRF:MB< AR=KHERLBL *:M>
KB:EL   
89 4:G 6 )B "??><MBO>G>LL H? FB<KH;B:E IK>MK>:MF>GM ;R >KBIHKBHILBL LN;O>K
FBLIHK: HG =B??>K>GM ;BHF:LL ?>>=LMH<DL BHK>LHNK 1><AGHE  

89 ' )BN *) 4:G@  1HGGBL * %:;M>L>E:LLB> 5 )B:H . %N:G@ #NG@:E IK>
MK>:MF>GM H? LPBM<A@K:LL ?HK BFIKHO>= L:<<A:KBi<:MBHG :G= LBFNEM:G>HNL >GSRF>
IKH=N<MBHG BHK>LHNK 1><AGHE   
89 )B' 0NG #A 6Q 6N:G 7R 6:G 5? )BN "??><M H? ;BHEH@B<:E IK>MK>:MF>GM
PBMA 1K:F>M>L ABKLNM: RC HG >GSRF:MB< AR=KHERLBL H? <HKG LMHO>K &GM BH=>M>KBHK
BH=>@K:=   
89 '4 )>> (0 $P:D '6 -:KD *' -:KD !% AHB * (PHG >M :E BHEH@B
<:E IK>MK>:MF>GM H? LH?MPHH= -BGNL =>GLBjHK: ;R MAK>> PABM> KHM ?NG@B ' *B<KH
;BHE   
89 ' 0AB *0 ABGG // 0A:KF:0ABO:II: *B<KH;B:E IK>MK>:MF>GM H? <HMMHG
LM:EDL ;R LHEB= LM:M> <NEMBO:MBHG H? -A:G>KH<A:>M> <AKRLHLIHKBNF BHK>LHNK 1><A
GHE   
89 ' 0AB // 0A:KF:0ABO:II: *0 ABGG *B<KH;B:E IK>MK>:MF>GM H? <HMMHG
LM:EDL ;R LN;F>K@>= <NEMBO:MBHG H? -A:G>KH<A:>M> <AKRLHLIHKBNF BHK>LHNK
1><AGHE   
89 $* /:LAB= *' !NK:G->G: / /:AF:GIHNK ! 0:IL?HK= 1! N@@ !>EB@GB
i<:MBHG :G= >GA:G<>= @:L K>E>:L> ?KHF LHBE <HGM:BGBG@ EB@GH<>EENEHL> ;R MK>:M
F>GM PBMA ;:<M>KB:E EB@GBG =>@K:=>KL ' IIE *B<KH;BHE   
89 4 5N 0 #N 7 6:G@ ' )N $NH /HG@;H &FIKHO>= F>MA:G> IKH=N<MBHG ?KHF
<HKG LMK:P ;R FB<KH:>KH;B< IK>MK>:MF>GM PBMA : INK> ;:<M>KB: LRLM>F BHK>LHNK
1><AGHE   
89 # )_ ' 'B ) 0A:H - %> :<M>KB:E ;BH:N@F>GM:MBHG ?HK BFIKHOBG@ F>MA:G> :G=
AR=KH@>G IKH=N<MBHG ?KHF FB<KH:E@:> BHM><AGHE BH?N>E   

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
89 %( 0A:KF: 5N 4 .BG BHEH@B<:E IK>MK>:MF>GM H? EB@GH<>EENEHLB< ;BHF:LL
?HK ;BH?N>EL :G= ;BHIKH=N<ML :G HO>KOB>P 4:LM> BHF:LL 3:EHK  AMMIL
=HBHK@LR
89 0# #N # 4:G@ 57 6N:G 7* 6:G@ 0' )NH 0 4:G@ >M :E 1A> MA>K
FHIABEB< `  FB<KH:>KH;B< IK>MK>:MF>GM H? <HKG LMK:P ?HK :G:>KH;B< =B@>LMBHG
BHK>LHNK 1><AGHE   
89 6 A>G *! :B '0 A:G@ &FIKHOBG@ FB<KH:E@:E HBE <HEE><MBG@ >?i<B>G<R
;R IK>MK>:MBG@ MA> FB<KH:E@:E <>EE P:EE PBMA =>LMKN<MBO> ;:<M>KB: BH<A>F "G@ '
  
89 *! :B 6 A>G 4  )N %- 4:G 0% %H '0 A:G@ "GA:G<BG@ MA>
HBE >QMK:<MBHG >?i<B>G<R H? AEHK>EE: ONE@:KBL PBMA <>EE=BLKNIMBO> IK>MK>:MF>GM
NLBG@ :<MBO> >QMK:<>EENE:K LN;LM:G<>L ?KHF :<BEENL MANKBG@B>GLBL &1/&$
BH<A>F "G@ '   
89 0 %> 5 #:G +/ (:MNDNKB 5 6N:G # 4:G@ / $NH "GA:G<>= F>MA:G>
IKH=N<MBHG ?KHF FB<KH:E@:E ;BHF:LL ;R :G:>KH;B< ;BHIK>MK>:MF>GM BHK>LHNK
1><AGHE   
89  4>G 5 6N:G .5 )B ' )BN ' />G 5 4:G@ >M :E HFI:KBLHG :G= >O:EN:
MBHG H? <HG<NKK>GM L:<<A:KBi<:MBHG :G= :G:>KH;B< =B@>LMBHG H? +:IB>K @K:LL :?M>K
IK>MK>:MF>GM ;R MAK>> FB<KH;B:E <HGLHKMB: BHK>LHNK 1><AGHE  

89 ! (:ER:GB (* )>> 10 (BF ' )B 00 !ABF:G 6  (:G@ >M :E *B<KH;B:E
<HGLHKMB: ?HK L:<<A:KBi<:MBHG H? PHH=R ;BHF:LL :G= >MA:GHE ?>KF>GM:MBHG #N>E
  
89 00 !ABF:G '/ %:P ! (:ER:GB 3  (:EB: 6  (:G@ '( )>> 0BFNEM:G>
HNL IK>MK>:MF>GM :G= L:<<A:KBi<:MBHG @K>>G M><AGHEH@R ?HK >GA:G<>= LN@:K
RB>E=L ?KHF ;BHF:LL NLBG@ : ?NG@:E <HGLHKMBNF BHK>LHNK 1><AGHE  

89 *" !:O>R $ ,MHHE> *B<KH;B:E ;BHiEFL ?KHF ><HEH@R MH FHE><NE:K @>G>MB<L
*B<KH;BHE *HE BHE />O   
89 ' 0AHG@ */ 'BF>G>S !B:S % HEEBGL 1HP:K=L LRGMA>MB< FB<KH;B:E <HGLHKMB:
?HK ;BHIKH<>LLBG@ NKK ,IBG BHM><AGHE   
89 ( K>GG>K ) 6HN #% KGHE= "G@BG>>KBG@ FB<KH;B:E <HGLHKMB: : G>P ?KHGMB>K
BG LRGMA>MB< ;BHEH@R 1K>G= BHM><AGHE   
89 * !:LAM;:G % 0<AK:?M 4 .BG #NG@:E ;BH<HGO>KLBHG H? EB@GH<>EENEHLB<
K>LB=N>L HIIHKMNGBMB>L  I>KLI><MBO>L &GM ' BHE 0<B   
89 1/ 7NKHm 0 5BJN>L 4/ NKMBL HGLHKMB:F>=B:M>= ;BHIKH<>LLBG@ H? <>EEN
EHL> MH >MA:GHE PBMA : LRF;BHMB< EHLMKB=BNF IARMH?>KF>GM:GLR>:LM <H<NEMNK>
BHM><AGHE BH?N>E   
89 00 EB "# ;HFHAK: ' 0NG "??><MBO> ;BHIK>MK>:MF>GM H? L:P=NLM P:LM>
PBMA : GHO>E FB<KH;B:E <HGLHKMBNF ?HK >GA:G<>= ;BHF>MA:G:MBHG BHK>LHNK
1><AGHE   
89 5 #:G@ . )B 6 )BG 5 )BG 6 !:B 7 $NH >M :E 0<K>>GBG@ H? : FB<KH;B:E <HG
LHKMBNF ?HK L>E><MBO> =>@K:=:MBHG H? EB@GBG ?KHF MK>> MKBFFBG@L BHK>LHNK 1><A
GHE   
89 *N[HS %B=:E@H * 7:I:M: ! '>BLHG /BJN>EF> * /BO:L 2L> H? <>EEN
EHERMB< F:KBG> ;:<M>KB: ?HK >GSRF:MB< IK>MK>:MF>GM BG FB<KH:E@:E ;BH@:L IKH=N<
MBHG IIE "GOBKHG *B<KH;BHE   
89 * *:DB 0 &LDA:DHO: 1 7A:G@ 4 .BG :<M>KB:E <HGLHKMB: <HGLMKN<M>= ?HK MA>
=><HFIHLBMBHG H? @:O> ;BHF:LL BH>G@BG>>K>=   
89 ' %N 6 5N> % $NH *M $:H ' )B 0 7A:G@ >M :E !>LB@G :G= <HFIHLBMBHG
H? LRGMA>MB< ?NG@:E;:<M>KB:E FB<KH;B:E <HGLHKMB: MA:M BFIKHO> EB@GH<>EENEHERMB<
>GSRF> :<MBOBMR BHK>LHNK 1><AGHE   
89 '' *BGMR *" 0BG@>K 0 0<AHES % :> '% AG " #HLM>K >M :E !>
LB@G :G= <A:K:<M>KBS:MBHG H? LRGMA>MB< ?NG@:E;:<M>KB:E <HGLHKMB: ?HK =BK><M IKH
=N<MBHG H? BLH;NM:GHE ?KHF <>EENEHLB< ;BHF:LL -KH< +:ME <:= 0<B 20
  
89 4 7AHG@ 7 7A:G@ 6 )NH 0 0NG 4 .B:H * 5B:H "??><M H? ;BHEH@B<:E IK>
MK>:MF>GML BG >GA:G<BG@ <HKG LMK:P ;BH@:L IKH=N<MBHG BHK>LHNK 1><AGHE
  
89 /H=KB@N>S  E:LP:= ( >GRHNGBL  ,E:;B -K>MK>:MF>GM M><AGBJN>L NL>=
BG ;BH@:L IKH=N<MBHG ?KHF @K:LL />G>P 0NLM:BG "G>K@R />O  

89 02 ):KL>G ( %CHKM$K>@>KL>G % 3:SB?>ADAHK:G '* 1KBHEH H>GLBEBG@ H?
LMK:P PBMA LN@:K ;>>M E>:O>L BG<K>:L>L MA> F>MA:G> RB>E= ?KHF LMK:P BHK>LHNK
1><AGHE   
89 4 !>P:K - *<!HG:E= / 4ABMM>G;NKR 1A> AR=KHERLBL H? @K:LL A>FB<>EENEHL>L
=NKBG@ >GLBE:@> ' 0<B #HH= @KB<   
89 6 7A>G@ 6N 60 A>G@ / 7A:G@  '>GDBGL '0 3:G=>K$A>RGLM "??><ML H?
>GLBE:@> HG LMHK:@> :G= >GSRF:MB< =>@K:=:;BEBMR H? LN@:K ;>>M INEI BHK>LHNK
1><AGHE   
89 !+ 1AHFILHG '* :KG>L 1- %HN@AMHG "??><M H? :==BMBHGL HG >GLBEBG@ :G=
FB<KH;B:E <HFFNGBMR H? L>G>L<>= PA>:M LMK:P IIE BH<A>F BHM><AGHE
  
89 ! $:EE>@HL % 4>=PBML<AD: ) *H>EE>K  7>AGL=HK? 4 0MBGG>K "??><M H? I:K
MB<E> LBS> K>=N<MBHG :G= >GLBEBG@ ?>KF>GM:MBHG HG ;BH@:L ?HKF:MBHG :G= LBE:@>
JN:EBMR H? PA>:M LMK:P BHK>LHNK 1><AGHE   
89 ' :KKHL/BHL  /HF:GZ $ $:KKHM>  ,K=:L BHF:LL LN@:K :G= ;BH>MA:GHE
IHM>GMB:E H? LP>>M <HKG $  BH>G>K@R  
89 1% />@:LL: 0 4HKMF:GG 0P>>M LHK@ANF :L : ;BH>G>K@R <KHI EBM>K:MNK> K>
OB>P BHF:LL BH>G>K@   
89 6 A>G // 0A:KF:0ABO:II: A>G "GLBEBG@ :@KB<NEMNK:E K>LB=N>L ?HK
;BH>MA:GHE IKH=N<MBHG IIE BH<A>F BHM><AGHE   
89 * F;R>'>GL>G (0 'HA:GL>G 1 !B=BHG 7 (T=TK '" 0<AFB=M 0 *>R>K
"GLBEBG@ :L ;BHEH@B<:E IK>MK>:MF>GM H? @K:LL ?>LMNEHEBNF ARDHK MA> >?
?><M H? <HFIHLBMBHG =KR F:MM>K :G= BGH<NE: HG <>EENEHL> <HGO>KMB;BEBMR BHF:LL
BH>G>K@   
89 ' -ET<B=H 0 :I:K>=: )B@GBGHERMB< >GSRF>L : ;BHM><AGHEH@B<:E :EM>KG:MBO> ?HK
;BH>MA:GHE IKH=N<MBHG BHK>LHNK BHIKH<>LL   
89 * L@A>K 7 AF:= %*+ &J;:E ED:EB :G= >GSRF:MB< =>EB@GBi<:MBHG H? LN@
:K<:G> ;:@:LL> MH >QIHL> <>EENEHL> IHERF>KL ?HK L:<<A:KBi<:MBHG :G= ;BH>MA:GHE
IKH=N<MBHG &G= <KHI -KH=   
89 3:G>@:L  %>KGHG ' :KME>MM "GSRF:MB< :G= HK@:GB< :<B= IK>MK>:MF>GM H?
L>:P>>= >??><M HG K>=N<BG@ LN@:KL IKH=N<MBHG :G= HG ;BH@:L BGAB;BMBHG &GM '
F;B>GM "G>K@R   
89  *:A=R ) *>G=>S * :EE>LM>KHL $HGSTE>S#>KGTG=>S "GA:G<>= F>MA:G>
IKH=N<MBHG H? AEHK>EE: ONE@:KBL :G= AE:FR=HFHG:L K>BGA:K=MBB ;R AR=KHERMB<
>GSRF>L :==BMBHG "G>K@ HGO>KL *:G:@   
89 6* 6NG !% (BF 6( ,A %0 0ABG (4 'NG@ IIEB<:MBHG H? : GHO>E >G
SRF:MB< IK>MK>:MF>GM NLBG@ <KN=> AR=KHERMB< >QMK:<>EENE:K >GSRF> LHENMBHG MH FB
<KH:E@:E ;BHF:LL ?HK =:KD ?>KF>GM:MBO> AR=KH@>G IKH=N<MBHG BHK>LHNK 1><AGHE
  
89   #N 1  %NG@ '6 A>G % 0N 41 4N %R=KHERLBL H? FB<KH:E@:>
<>EE P:EEL ?HK IKH=N<MBHG H? K>=N<BG@ LN@:K :G= EBIB= >QMK:<MBHG BHK>LHNK 1><A
GHE   
89 % 7A>G@ ' 6BG 7 $:H % %N:G@ 5 'B !HN !BLKNIMBHG H? AEHK>EE: ONE@:KBL
<>EEL ?HK MA> K>E>:L> H? ;BH=B>L>EIKH=N<BG@ EBIB=L : <HFI:KBLHG H? @KBG=BG@ NEMK:
LHGB<:MBHG ;>:= FBEEBG@ >GSRF:MB< ERLBL :G= FB<KHP:O>L IIE BH<A>F
BHM><AGHE   
89 (% (BF &0 AHB %* (BF 0$ 4B %' :> BH>MA:GHE IKH=N<MBHG ?KHF
MA> GNMKB>GM LMK>LLBG=N<>= FB<KH:E@: AEHK>EE: ONE@:KBL ;R >GSRF:MB< AR=KHERLBL
:G= BFFH;BEBS>= R>:LM ?>KF>GM:MBHG BHK>LHNK 1><AGHE   
89 0- AHB *1 +@NR>G 0' 0BF "GSRF:MB< IK>MK>:MF>GM H? AE:FR=HFHG:L
K>BGA:K=MBB ;BHF:LL ?HK >MA:GHE IKH=N<MBHG BHK>LHNK 1><AGHE  

89  *:A=R ) *>G=>S 0 E:G<H * :EE>LM>KHL $HGSTE>S#>KGTG=>S -KHM>:L>
<>EE P:EE =>@K:=:MBHG H? AEHK>EE: ONE@:KBL >??><M HG F>MA:G> IKH=N<MBHG BHK>
LHNK 1><AGHE   
89 $ BN=:= , /N;BE:K ) S\<:K 1HKH * >: d 1HKK>L >M :E ->K?HKF:G<>
H? :G >GSRF:MB< >QMK:<M BG HMKR<H<<NL ;K:NGBB <>EE P:EE =BLKNIMBHG ' BHL<B BH
>G@   
89 " "ABF>G ' %HEF+B>EL>G * -HNEL>G '" H>ELF:G= &GjN>G<> H? =B??>K
>GM IK>MK>:MF>GM KHNM>L HG MA> :G:>KH;B< =B@>LMBHG H? : iE:F>GMHNL :E@:> />
G>P "G>K@   
89 ( 7B>FBnLDB & /HF:GHPLD: * (HP:ELD: "GSRF:MB< IK>MK>:MF>GM H? EB@GH<>E
ENEHLB< P:LM>L MH BFIKHO> ;BH@:L IKH=N<MBHG 4:LM> *:G:@  

89 - (:NLABD  *:EBD 0 0A:KF: 3>KFB<HFIHLMBG@ :G ><H?KB>G=ER HIMBHG ?HK ?>K
F>GM:MBHG :G= =R> =><HEHNKBS:MBHG P:LM> =BLIHL:E E>:G0HBE BK 4:M>K
  
89 0( -K:C:I:MB  A:MM:<A:KR:  *:EBD 3( 3BC:R -K>MK>:MF>GM H? :E@:E ;BH
F:LL NLBG@ ?NG@:E <KN=> >GSRF>L E@:E />L   
89 0( -K:C:I:MB  *:EBD 3 3BC:R 1 0K>>DKBLAG:G "GA:G<>= F>MA:G> IKH=N<MBHG
?KHF :E@:E ;BHF:LL MAKHN@A LAHKM =NK:MBHG >GSRF:MB< IK>MK>:MF>GM :G= <H=B@>L
MBHG PBMA <:K;HG KB<A P:LM> /0 =O  
89 * !:LAM;:G % 0<AK:?M 1 0R>= 4 .BG #NG@:E ;BH=>@K:=:MBHG :G= >GSR
F:MB< FH=Bi<:MBHG H? EB@GBG &GM ' BH<A>F *HE BHE   
89 !40 4HG@ 0MKN<MNK> :G= :<MBHG F><A:GBLF H? EB@GBGHERMB< >GSRF>L IIE
BH<A>F BHM><AGHE   
89 ' )B % 6N:G ' 6:G@ :<M>KB: :G= EB@GBG =>@K:=:MBHG #KHGM BHE ABG:
  
89 0 *:CNF=:K 1 )NDD ', 0HE;B:MB 0 :N>K 0( +:BK '" KHG:G >M :E /HE>L
H? LF:EE E:<<:L>L ?KHF 0MK>IMHFR<>L BG EB@GBG =>@K:=:MBHG BH<A>F  

89 3 />G@:G:MA:G *% $HE= 0I><MK:E <A:K:<M>KBS:MBHG H? MA> HQB=BS>= LM:M>L H?
EB@GBG I>KHQB=:L> :G >QMK:<>EENE:K A>F> >GSRF> ?KHF MA> PABM> KHM ;:LB=
BHFR<>M> -A:G>KH<A:>M> <AKRLHLIHKBNF BH<A>F   
89 "* 4HHEKB=@> *BQ>= >GSRF> LRLM>FL ?HK =>EB@GBi<:MBHG H? EB@GH<>EENEHLB< ;BH
F:LL :M:ERLML  
89 )- AKBLMHIA>K  6:H 6 'B )B@GBG ;BH=>@K:=:MBHG PBMA E:<<:L>F>=B:MHK LRL
M>FL #KHGM "G>K@ />L  
89 / HNK;HGG:BL * -:B<>  #K>B>KFNMA " H=B> 0 HKG>F:G />:<MBOBMB>L H?
O:KBHNL F>=B:MHKL :G= E:<<:L>L PBMA DK:?M INEI :G= EB@GBG FH=>E <HFIHNG=L
IIE "GOBKHG *B<KH;BHE   
89 $:EEB - $>GMBEB A>FB<:E F>LL>G@>KL F>=B:M>= HQB=:MBHGL PBMA MA> >GSRF>
E:<<:L> ' -ARL ,K@ A>F   
89 0 !>L:B +BMR:G:G= *B<KH;B:E E:<<:L>L :G= MA>BK :IIEB<:MBHGL : K>OB>P LB:G
' BHM><AGHE   
89 - BGH= (2 ':GN / 0BG=AN  -:G=>R %R=KHERLBL H? EB@GH<>EENEHLB< ;BHF:LL
?HK ;BH>MA:GHE IKH=N<MBHG BG - LAHD ) AKBLMB:G / 0M>O>G !
E:N=>$BEE>L $ "=@:K= "=L BH?N>EL :EM>KG:MBO> ?>>=LMH<DL :G= <HGO>KLBHG
IKH<>LL>L <:=>FB< -K>LL FLM>K=:F  II 
89 ( 3:EEB % 4:KBBLAB *% $HE= ,QB=:MBHG H? FHGHF>MAHQRE:M>= :KHF:MB< <HF
IHNG=L ;R EB@GBG I>KHQB=:L> KHE> H? O>K:MKRE :E<HAHE BG EB@GBG ;BH=>@K:=:MBHG
BH<A>F   
89 - %:KO>R ' -:EF>K ,QB=:MBHG H? IA>GHEB< <HFIHNG=L ;R EB@GBG:L> ' BHM><A
GHE   
89 $ #>BCHH *1 *HK>BK: - EO:K>S 1 )^ A:N '* )>F: "O:EN:MBHG H? MA>
>GSRF> F:G@:G>L> I>KHQB=:L> BG :G BG=NLMKB:E L>JN>G<> ?HK MA> EB@GBG HQB

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
=:MBHG :G= ;E>:<ABG@ H? >N<:ERIMNL DK:?M INEI ' IIE -HERF 0<B  

89 (" %:FF>E ( '>GL>G *! *HSN<A )) ):G=N<<B * 1B>G " ->:L> )B@GB
GHERLBL ;R : INKBi>= EB@GBG I>KHQB=:L> ' BHE A>F   
89 $3 />==R * 0KB=A:K *% $HE= E>:O:@> H? GHGIA>GHEB< β =B:KREIKHI:G>
EB@GBG FH=>E =BF>KL ;R F:G@:G>L> I>KHQB=:L> ?KHF >OB=>G<> ?HK : AR=KH@>G :;
LMK:<MBHG F><A:GBLFIA:G>KH<A:>M> <AKRLHLIHKBNF >OB=>G<> ?HK : AR=KH@>G :;
LMK:<MBHG F><A:GBLF "NK ' BH<A>F   
89 2 1NHK % 4:KBBLAB %" 0<AH>F:D>K *% $HE= ,QB=:MBHG H? IA>GHEB< :KRE
@ER<>KHE ;>M::KRE >MA>K EB@GBG FH=>E <HFIHNG=L ;R F:G@:G>L> I>KHQB=:L> ?KHF
-A:G>KH<A:>M> <AKRLHLIHKBNF HQB=:MBO> <E>:O:@> H? :G :EIA:<:K;HGRE FH=>E
<HFIHNG= BH<A>F   
89 4 :H 6 #NDNLABF: ( '>GL>G 'K * *H>G (" %:FF>E ,QB=:MBO>
=>@K:=:MBHG H? GHGIA>GHEB< EB@GBG =NKBG@ EBIB= I>KHQB=:MBHG ;R ?NG@:E F:G
@:G>L> I>KHQB=:L> #"0 )>MM   
89 + (:IB<A (1 0M>??>G * %H?KB<AM>K  %:M:DD: &GOHEO>F>GM H? EBIB= I>KHQB
=:MBHG BG MA> =>@K:=:MBHG H? : GHGIA>GHEB< EB@GBG FH=>E <HFIHNG= ;R F:G
@:G>L> I>KHQB=:L> H? MA> EBMM>K=><HFIHLBG@ ?NG@NL 0MKHIA:KB: <HKHGBEE:
BH<A>F BHIARL />L HFFNG   
89 !* (:MH + "EZ: * #ERMA>   )RGG -K>MK>:MF>GM H? EB@GH<>EENEHLB< ;BHF:LL
NLBG@ #>GMHG <A>FBLMKR BHK>LHNK 1><AGHE   
89 3 $\F>S1HKB;BH  $:K<Z:*:KMZG *' *:KMZG>S d1 *:KMZG>S # $NBEEWG
&G=N<MBHG H? >QMK:<>EENE:K AR=KHQRE K:=B<:E IKH=N<MBHG ;R PABM>KHM ?NG@B MAKHN@A
JNBGHG> K>=HQ <R<EBG@ IIE "GOBKHG *B<KH;BHE   
89  /BML<ADHm !><:R F><A:GBLFL H? ;KHPGKHM ?NG@B 1><AGB<:E />L>:K<A >GMK>
H? #BGE:G= "LIHH  AMMIPPPOMMiBG?I=?IN;EB<:MBHGL-I=?
89   $BE;>KM !* (BG@  1AHF:L 1A> HQB=:MBHG H? LHF> IHERL:<<A:KB=>L ;R
MA> AR=KHQRE K:=B<:E :G "0/ BGO>LMB@:MBHG :K;HAR=K />L   
89 1( (BKD / &;:<A *! *HSN<A % HGG>K ) %B@AE>R A:K:<M>KBLMB<L H? <HM
MHG <>EENEHL> =>IHERF>KBS>= ;R : ;KHPGKHM ?NG@NL ;R :<B= HK ;R <A>FB<:E HQB
=:GML %HES?HKL<A &GM ' BHE A>F -ARL 1><AGHE 4HH=   
89 ( *B<A:ELD: ( *B:S>D ) (KSRLM>D 0 )>=:DHPB<S &GjN>G<> H? IK>MK>:MF>GM
PBMA #>GMHGL K>:@>GM HG ;BH@:L IKH=N<MBHG :G= F>MA:G> RB>E= ?KHF EB@GH<>EEN
EHLB< ;BHF:LL BHK>LHNK 1><AGHE   
89 5 4:G@ 7 )B ' 0N 6 1B:G 5 +BG@ % %HG@ >M :E )RLBL H? : K>=MB=> <:NLBG@
:E@: E>Q:G=KBNF M:F:K>GL> <:NL>= ;R ;:<M>KB: ?KHF BML IAR<HLIA>K> BHE HG
MKHE   
89 -:NE $ -HAG>KM &GM>K:<MBHGL H? MA> :E@B<B=:E ;:<M>KBNF (HK=B: :E@B<B=: PBMA
=B:MHFL K>@NE:M>= IKHM>:L> >Q<K>MBHG ?HK LI><Bi< :E@:E ERLBL -)H0 ,G>  
>
89 . E ;=:EE:A 1 +BQHG '/ #HKMP>G=>E 1A> >GSRF:MB< <HGO>KLBHG H? F:CHK
:E@:E :G= <R:GH;:<M>KB:E <:K;HAR=K:M>L MH ;BH>MA:GHE #KHGM "G>K@R />L  

89 ! BKG> 5 -:EHNF>M ) C]KGLLHG * EO>L  *:MMB:LLHG G:>KH;B< =B@>LMBHG
H? EBIB=KB<A P:LM>>??><ML H? EBIB= <HG<>GMK:MBHG />G>P "G>K@  

89 !- *:NKR:  0BG@E: 0 +>@B G HO>KOB>P H? D>R IK>MK>:MF>GM IKH<>LL>L ?HK
;BHEH@B<:E <HGO>KLBHG H? EB@GH<>EENEHLB< ;BHF:LL MH ;BH>MA:GHE  BHM><A  

89 0 HE:=H/H=KZ@N>S 1HJN>KH ' *:KMZG'NTK>S / 1K:O:BGB -
$:K<Z:"G<BG: "??><M H? MA>KF:E :<B= :ED:EBG> :G= :ED:EBG>I>KHQB=> IK>MK>:M
F>GML HG MA> ;BH<A>FB<:E F>MA:G> IHM>GMB:E :G= DBG>MB<L H? MA> :G:>KH;B< =B@>L
MBHG H? PA>:M LMK:P :G= LN@:K<:G> ;:@:LL> BHK>LHNK 1><AGHE  

89 ! 0:EO:<A^:  -KB>MH * )\I>S;>E:BK:L 1 )N A:N d1 *:KMZG>S *'
*:KMZG>S #NG@:E IK>MK>:MF>GM :G :EM>KG:MBO> BG L><HG=@>G>K:MBHG >MA:GHE ?KHF
PA>:M LMK:P BHK>LHNK 1><AGHE   
89 00 %P:G@ 0' )>> %( (BF ', (: (' (BF %$ 0HG@ BH=>@K:=:MBHG
:G= L:<<A:KBi<:MBHG H? PHH= <ABIL H? -BGNL LMKH;NL :G= )BKBH=>G=KHG MNEBIB?>K:
;R PABM> KHM ?NG@B ' *B<KH;BHE BHM><AGHE   
89 - $>KO:BL - *HEBG 1A> KHE> H? P:M>K BG LHEB=LM:M> ?>KF>GM:MBHG BH<A>F "G@ '
  
89 // 0BG@A:GB: ( -:M>E / 0H<<HE  -:G=>R /><>GM :=O:G<>L BG LHEB=LM:M>
?>KF>GM:MBHG BH<A>F "G@ '   
89 * *NLM:?: 1$ -HNEL>G ( 0A>G@ #NG@:E IK>MK>:MF>GM H? KB<> LMK:P PBMA
-E>NKHMNL HLMK>:MNL :G= 1KB<AH=>KF: K>>L>B MH >GA:G<> F>MA:G> IKH=N<MBHG NG
=>K LHEB=LM:M> :G:>KH;B< =B@>LMBHG IIE "G>K@   
89 (0*0 /:@A:O:K:H 13 /:G@:G:MA:G +$ (:K:GMA 0HF> >G@BG>>KBG@ :LI><ML
H? LHEB=LM:M> ?>KF>GM:MBHG BH<A>F "G@ '   
89 &! />B= BHEH@B<:E =>EB@GBi<:MBHG H? :LI>G PHH= ;R LHEB=LM:M> ?>KF>GM:MBHG
PBMA MA> PABM>KHM ?NG@NL *>KNEBNL MK>F>EEHLNL IIE "GOBKHG *B<KH;BHE
  
89 &! />B= 0HEB=LM:M> ?>KF>GM:MBHGL ?HK ;BHEH@B<:E =>EB@GBi<:MBHG "GSRF *B<KH;
1><AGHE   
89 )* *:KK: 0*= ,EBO>BK:)HG@:MMB / 0H:K>L '*= )BF: #) ,EBO:K>L #
*HK>BK: &GBMB:E I% H? F>=BNF :??><ML HK@:GB< :<B=L IKH=N<MBHG ;NM =H GHM :??><M
IAHLIA:M> LHEN;BEBS:MBHG K:S ' *B<KH;BHE   
89 $ $>B@>K % K:G=E $ #NKK>K / 0<ANEBG 1A> >??><M H? <HII>K HG MA> :<MBOBMR H?
<>EENE:L> :G= β@EN<HLB=:L> BG MA> IK>L>G<> H? FHGMFHKBEEHGBM> HK EFHGMFHKBE
EHGBM> 0HBE BHE BH<A>F   
89 " @HLBG " ,=B>K 0HEB=LM:M> ?>KF>GM:MBHG EB@GBG =>@K:=:MBHG :G= K>LNEMBG@ =B
@>LMB;BEBMR H? PA>:M LMK:P ?>KF>GM>= ;R L>E><M>= PABM>KHM ?NG@B IIE *B<KH;BHE
BHM><AGHE   
89 ) 7A:H $) :H ' 4:G@ %6 />G ! !HG@ 7+ )BN >M :E #NG@:E IK>
MK>:MF>GM H? <HKGLM:ED PBMA -A:G>KH<A:>M> <AKRLHLIHKBNF ?HK >GA:G<BG@ >GSR
F:MB< L:<<A:KBi<:MBHG :G= AR=KH@>G IKH=N<MBHG BHK>LHNK 1><AGHE  

89 */ $KN;>KKNGANF>K ' '>KG>R " 7HA:K * +NLL;:NF>K %B>@>K $
H<AF:GG >M :E <NMH=>LFNL H;EBJNNL :L : ;>G<AF:KD LMK:BG ?HK >O:EN:MBG@
F>MA:G> IKH=N<MBHG ?KHF FB<KH:E@:> BGjN>G<> H? =B??>K>GM LMHK:@> :G= IK>MK>:M
F>GM F>MAH=L HG ;BH@:L RB>E= E@:E />L   
89 *N[HS %B=:E@H * 7:I:M: ! '>BLHG /BJN>EF> * /BO:L 2L> H? <>EEN
EHERMB< F:KBG> ;:<M>KB: ?HK >GSRF:MB< IK>MK>:MF>GM BG FB<KH:E@:E ;BH@:L IKH=N<
MBHG IIE "GOBKHG *B<KH;BHE  8"* 9
89 # ,F>MMH $ .NBKH@: - -o>GBgD: / 4ABMMHG  '>??>KLHG / 3BEE: &FI:<ML H?
FB<KH:E@:> IK>MK>:MF>GML ?HK BFIKHO>= :G:>KH;B< =B@>LMBHG MA>KF:E MK>:MF>GM
MA>KF:E AR=KHERLBL NEMK:LHNG= :G= >GSRF:MB< AR=KHERLBL 4:M>K />L  

89 5 6:G@ 1 4:G@ )+ $:H %' 6BG 5 )_ &LHE:MBHG B=>GMBi<:MBHG :G= <A:K
:<M>KBS:MBHG H? EB@GBG=>@K:=BG@ ;:<M>KB: ?KHF .BGEBG@ ABG: ' IIE *B<KH;BHE
  
89 0 %:AGD> ;>G=KHMA 1 ):G@>K #* H=H[>K - /:FF * -HK<:K >M :E
HFIE>M> @>GHF> L>JN>G<> H? : G>P /NFBGH<H<<:<>:> ;:<M>KBNF BLHE:M>= ?KHF
:G:>KH;B< ;BHF:LL AR=KHERLBL $>GHF> GGHNG<   >
89 $$ AHG@ 5' %N:G@ '% !B !7 5N 6  %> 6+ ->B >M :E BH=>@K:
=:MBHG H? :ED:EB EB@GBG ;R : G>PER BLHE:M>= /AH=H<H<<NL IRKB=BGBOHK:GL
72 BHIKH<>LL BHLRLM "G@   
89 * :KK:@TG1KBGB=:= ' :KKBEEH/>R>L $ NBMK\G %R=KHERLBL H? FB<KH:E@:E ;BH
F:LL NLBG@ KNFBG:E FB<KHHK@:GBLFL :L : IK>MK>:MF>GM MH BG<K>:L> F>MA:G> K>
<HO>KR BHK>LHNK 1><AGHE   
89 ' $BFWG>S ! @N:=H  HNS:L ' #>KK>K  0><H 2L> H? KNF>G FB<KHHK
@:GBLFL MH ;HHLM MA> :G:>KH;B< ;BH=>@K:=:;BEBMR H? FB<KH:E@:> E@:E />L
  
89 ( 0KBO:LM:O: - @K:P:E  /:ABF:G -K>MK>:MF>GM :G= IKH=N<MBHG H?
;BH>MA:GHE ?KHF =B??>K>GM EB@GH<>EENEHLB< ;BHF:LL &GM '   
89 . 4:G@ 7 4:G@ # 0A>G ' %N # 0NG ) )BG >M :E -K>MK>:MBG@ EB@GH<>EENEHLB<
;BHF:LL ;R MA> <HG<>GMK:M>= IAHLIAHKB< :<B= IENL AR=KH@>G I>KHQB=> -%- ?HK
>GSRF:MB< AR=KHERLBL >O:EN:MBG@ MA> IK>MK>:MF>GM j>QB;BEBMR HG ?>>=LMH<DL :G=
I:KMB<E> LBS>L BHK>LHNK 1><AGHE   
89 ' A:G@ ' #:G 5 4>G 0<K>>GBG@ H? ?NG@B <:I:;E> H? AB@AER L>E><MBO> =>@K:
=:MBHG H? EB@GBG BG KB<> LMK:P &GM BH=>M>KBHK BH=>@K:=   
89 * $:K<Z:1HKK>BKH * )\I>S;>E:BK:L 1 )N A:N '* )>F: #NG@:E IK>
MK>:MF>GM H? :@KB<NEMNK:E K>LB=N>L ?HK ;BH>MA:GHE IKH=N<MBHG &G= KHI -KH=
  
89 # *: + 6:G@ 5N % 6N ' 4N 5 7A:G@ HF;BG:MBHG H? ;BHEH@B<:E IK>
MK>:MF>GM PBMA FBE= :<B= IK>MK>:MF>GM ?HK >GSRF:MB< AR=KHERLBL :G= >MA:GHE IKH
=N<MBHG ?KHF P:M>K AR:<BGMA BHK>LHNK 1><AGHE   
89 * E>Q:G=KHIHNEHN $ GMHGHIHNEHN " #K:@DHN & +M:BDHN $ )R;>K:MHL
#NG@:E IK>MK>:MF>GM H? PBEEHP L:P=NLM :G= BML <HF;BG:MBHG PBMA :ED:EBG> MK>:M
F>GM ?HK >GA:G<BG@ ;BH@:L IKH=N<MBHG ' "GOBKHG *:G:@   
89 6 !:B * 0B 6 A>G + 7A:G@ * 7AHN . )B:H >M :E HF;BG:MBHG H? ;BHEH@B
<:E IK>MK>:MF>GM PBMA +:,%2K>: IK>MK>:MF>GM :M <HE= M>FI>K:MNK> MH >GA:G<>
>GSRF:MB< AR=KHERLBL H? KB<> LMK:P BHK>LHNK 1><AGHE   
89 * *NLM:?: 1$ -HNEL>G 6 5B: ( 0A>G@ HF;BG:MBHGL H? ?NG@:E :G= FBEEBG@
IK>MK>:MF>GML ?HK >GA:G<BG@ KB<> LMK:P ;BH@:L IKH=N<MBHG =NKBG@ LHEB=LM:M>
:G:>KH;B< =B@>LMBHG BHK>LHNK 1><AGHE   
89 $ )B % A>G 0RG>K@BLMB< F><A:GBLF H? LM>:F >QIEHLBHG <HF;BG>= PBMA ?NG@:E
MK>:MF>GM ;R -A>EEBGNL ;:NFBB ?HK MA> IK>MK>:MF>GM H? <HKG LM:ED BHF:LL BH>G
>K@   
89 5 $NB $ 4:G@ * %N 6 6:G HF;BG>= ?NG@:E :G= FBE= :<B= IK>MK>:MF>GM H?
$ER<RKKABS: NK:E>GLBL K>LB=N> ?HK >GA:G<BG@ >GSRF:MB< AR=KHERLBL :G= HBE IKH=N<
MBHG BH/>LHNK<>L   
89 ** &LAHE: &LKHB *' 1:A>KS:=>A "??><M H? ?NG@:E :G= IAHLIAHKB< :<B= IK>
MK>:MF>GM HG >MA:GHE IKH=N<MBHG ?KHF HBE I:EF >FIMR ?KNBM ;NG<A>L ,-"#
BHK>LHNK 1><AGHE   
89 % 6:G@ ( 4:G@ 4 4:G@ /  0NG &FIKHO>= ;BH<HGO>KLBHG H? IHIE:K ;R
LRG>K@BLMB< MK>:MF>GML PBMA PABM>KHM ?NG@NL 1K:F>M>L O>ENMBG: ! IK>MK>:M
F>GM :G= :ED:EBG> ?K:<MBHG:MBHG BHK>LHNK 1><AGHE   
89 4:G 6 )B "??><M H? AHM P:M>K >QMK:<MBHG :G= EBJNB= AHM P:M>K IK>MK>:MF>GM HG
MA> ?NG@:E =>@K:=:MBHG H? ;BHF:LL ?>>=LMH<DL BHK>LHNK 1><AGHE  

89 01 1AHFL>G '" )HG=H[H * F;R>'>GL>G 0 %>BLD> 7 (T=TK 0 *>R>K
HF;BG:MBHG H? >GLBEBG@ :G= ?NG@:E =>EB@GBi<:MBHG :L >??><MBO> PA>:M LMK:P IK>
MK>:MF>GM BHM><AGHE BH?N>E   
89 7 0BF>G@ $ 0:<A: %$ &L:;>EE> / *:KB>+HYEE> - /;:L>= F>MAH= MH
JN:GMB?R ?NG@:E @KHPMA =NKBG@ IK>MK>:MF>GM H? EB@GH<>EENEHLB< ;BHF:LL ' *B<KH
;BHE *>MAH=L   
89 0 %> 5 #:G 0 )NH +/ (:MNDNKB / $NH "GA:G<>= MA> >G>K@R HNM<HF>L ?KHF
FB<KH:E@:E ;BHF:LL ;R MA> GHO>E ;BHIK>MK>:MF>GM "G>K@R HGO>KL *:G:@
  
89 / 0BG@A ' %N */ />@G>K '4 /HNG= ' /:EIA '+ 0:==E>K >M :E "G
A:G<>= =>EB@GBi<:MBHG H? LM>:FIK>MK>:M>= IHIE:K ;R : ;:<M>KB:E E:<<:L> 0<B />I
  

UNCORRECTED PROOF
&'(+*+:'2 +4+='(2+'4*!;9:'/4'(2+4+8-? +</+=9 >>>  >>>>>>
89 01 1AHFL>G '"$ )HG=H[H * F;R>'>GL>G 0 %>BLD> 7 (T=TK 0
*>R>K HF;BG:MBHG H? >GLBEBG@ :G= ?NG@:E =>EB@GBi<:MBHG :L >??><MBO> PA>:M
LMK:P IK>MK>:MF>GM BHM><AGHE BH?N>E   
89 ' )BN 00 0B=AN *) 4:G@  1HGGBL * %:;M>L>E:LLB> ' *:H >M :E "O:EN:
MBHG H? O:KBHNL ?NG@:E IK>MK>:MF>GM H? LPBM<A@K:LL ?HK >GA:G<>= L:<<A:KBi<:MBHG
:G= LBFNEM:G>HNL >GSRF> IKH=N<MBHG ' E>:G -KH=   
89 ( *: 7 /N:G -KH=N<MBHG H? : EB@GH<>EENEHERMB< >GSRF> LRLM>F ?HK LBFNEM:G>HNL
;BH=>EB@GBi<:MBHG :G= L:<<A:KBi<:MBHG H? <HKG LMHO>K >FIEHRBG@ <H<NEMNK> H?
?NG@B BHK>LHNK 1><AGHE   
89 )! (ANHG@ / (HG=H /! )>HG 1( GA 0 *>@NKH ( 0ABFBSN >M :E "??><M
H? <A>FB<:E ?:<MHKL HG BGM>@K:M>= ?NG@:E ?>KF>GM:MBHG H? LN@:K<:G> ;:@:LL> ?HK
>MA:GHE IKH=N<MBHG ;R : PABM>KHM ?NG@NL -AE>;B: LI *$ BHK>LHNK 1><AGHE
  
89 + -:KD %6 (BF 4 (HH % 6>H &$ AHB ,K@:GHLHEO IK>MK>:MF>GM PBMA
O:KBHNL <:M:ERLML ?HK >GA:G<BG@ >GSRF:MB< AR=KHERLBL H? IBM<A IBG> -BGNL KB@B=:
BHK>LHNK 1><AGHE   
89 4 :H 0NG / )BN / 6BG 5 4N HFI:KBLHG H? MA> >??><ML H? iO> IK>MK>:M
F>GM F>MAH=L HG >GA:G<BG@ MA> >GSRF:MB< =B@>LMB;BEBMR :G= >MA:GHE IKH=N<MBHG
?KHF LP>>M LHK@ANF ;:@:LL> BHK>LHNK 1><AGHE   
89 + 0:KD:K 0( $AHLA 0 :GG>KC>> ( BD:M BH>MA:GHE IKH=N<MBHG ?KHF :@KB
<NEMNK:E P:LM>L :G HO>KOB>P />G>P "G>K@   
89 5 6:G 7 4:G@ ( 7A:G@ * 0B * )BN ) A:B >M :E :<M>KB:>GA:G<>= =BENM>
:<B= IK>MK>:MF>GM H? EB@GH<>EENEHLB< ;BHF:LL BHK>LHNK 1><AGHE  

89 5 7A:H # ->G@ ( A>G@ ! )BN "GA:G<>F>GM H? MA> >GSRF:MB< =B@>LMB;BEBMR H?
LN@:K<:G> ;:@:LL> ;R :ED:EBI>K:<>MB< :<B= IK>MK>:MF>GM "GSRF *B<KH; 1><AGHE
  
89 * !> ): 1HKK> / *:KMZG0:FI>=KH e #BEE:M *" "N@>GBH  ETGJN>S *
%>KGTG=>S >M :E HFI:KBLHG H? MA> >?i<B>G<R H? ;:<M>KB:E :G= ?NG@:E E:<<:L>L BG
=>EB@GBi<:MBHG :G= =>MHQBi<:MBHG H? LM>:FIK>MK>:M>= EB@GH<>EENEHLB< ;BHF:LL ?HK
;BH>MA:GHE IKH=N<MBHG ' &G= *B<KH;BHE BHM><AGHE   
89 0 !>K>GG> ):K@>:N >KD:EHm  /HNLL>:N 4BEA>EF - %:M<A>K
+HGAR=KHERL:;E> F:<KHFHE><NE:K <HGLMBMN>GML ?KHF HNM>K P:EEL H? AEHK>EE:
?NL<: :G= +:GH<AEHKNF >N<:KRHMNF -ARMH<A>FBLMKR   
89 d 1HKK>L #$ #>KFHLH  /BG<\G ' :KM:<>D / HKC: ! '>BLHG A:EE>G@>L
?HK <HLM>??><MBO> FB<KH:E@:> :G:>KH;B< =B@>LMBHG BG /HE:G=H / #K:G<BL<:
"=L BH=>@K:=:MBHG >G@BG>>KBG@ :G= M><AGHEH@R &G1><A  II 
89 % 4:G@ /1 %BEE 1 7A>G@ 5 %N  4:G@ "??><ML H? ;:<M>KB:E <HFFNGBMB>L HG
;BH?N>EIKH=N<BG@ FB<KH:E@:> LMBFNE:MBHG BGAB;BMBHG :G= A:KO>LMBG@ KBM />O
BHM><AGHE   
89 - HANMLDRB " HNP>K BH@:L IKH=N<MBHG ?KHF :E@:> :G= <R:GH;:<M>KB:
MAKHN@A :G:>KH;B< =B@>LMBHG : />OB>P G:ERLBL G= />L>:K<A +>>=L BG '4
)>> "= !O:G<>= BH?N>EL G= BHIKH=N<ML 0IKBG@>K +>P 6HKD +>P 6HKD
+6  II 
89 *0 (BF '0 :>D 60 6NG 0' 0BF 0 -:KD 0  (BF %R=KH@>G IKH=N<
MBHG ?KHF AE:FR=HFHG:L K>BGA:K=MBB ;BHF:LL NLBG@ : MPHLM>I <HGO>KLBHG
IKH<>LL :G:>KH;B< <HGO>KLBHG :G= IAHMHLRGMA>MB< ?>KF>GM:MBHG &GM ' %R=KH@ "G
>K@R   
89 $* 0<HMM * DAM:K *' )>GMS /" 0P:G>R "G@BG>>KBG@ L<:E>NI :G= ><H
GHFB< :LI><ML H? ?NG@:E IK>MK>:MF>GM H? PHH= <ABIL "GOBKHGF>GM:EER ?KB>G=ER
M><AGHEH@B>L ?HK MA> INEI :G= I:I>K BG=NLMKR 'HAG 4BE>R  0HGL +>P 6HKD

89 1B:G 5? 7 #:G@ # $NH &FI:<M :G= IKHLI><MBO> H? ?NG@:E IK>MK>:MF>GM H? EB@GH
<>EENEHLB< ;BHF:LL ?HK >GSRF:MB< AR=KHERLBL BH?N>E BHIKH= BHK>iG  

89 6 4:G@ 1 *<EEBLM>K /NF>G FB<KH;>L >GSRF>L :G= ?>>= =B@>LMBHG: K>OB>P
LB:GNLM ' GBF 0<B   
89 0 R=BG " 6fE=fKfF , &G<>  &G<> /NF>G :G:>KH;B< ?NG@B <K>:M> G>P HIIHK
MNGBMB>L ?HK >GA:G<>= F>MA:G> IKH=N<MBHG ?KHF FB<KH:E@:> ;BHF:LL E@:E />L
  
89 * BE:E %* &J;:E % %N 4 4:G@ 5 7A:G@ *>M:;HEB< >G@BG>>KBG@ :G= >G
SRF>F>=B:M>= IKH<>LLBG@ : ;BHM><AGHEH@B<:E O>GMNK> MHP:K=L ;BH?N>E IKH=N<
MBHG: K>OB>P />G>P 0NLM:BG "G>K@R />O   
89 1 *:@H<A: % 7:;>= * 6:G@ ' 6NG % 7A:G@ 5 .B &FIKHO>F>GM H? BG
=NLMKB:EER BFIHKM:GM FB<KH;B:E LMK:BGL ;R @>GHF> LAN?jBG@ <NKK>GM LM:MNL :G= ?N
MNK> IKHLI><ML BHK>LHNK 1><AGHE   
89 " #HDNF %* 7:;>= . $NH ' 6NG % -:G@ 6 G >M :E *>M:;HEB< >G@B
G>>KBG@ H? ;:<M>KB:E LMK:BGL NLBG@ /&0-/ :L LRLM>FL ?HK ;BHLRGMA>LBL H?
O:EN>:==>= IKH=N<ML #HH= BHL<B  AMMIL=HBHK@C?;BH

89 ' *HG<:=: ' 1:F:RH  :K=HG: &GM>@K:MBG@ iKLM L><HG= :G= MABK= @>G
>K:MBHG ;BHK>iG>KB>L BG<HKIHK:MBG@ FB<KH:E@:> BGMH MA> LN@:K<:G> ;BHK>iG>KR
A>F "G@ 0<B   
89 * *:G=>@:KB 0 #:KS:= '# $]K@>GL /><>GM MK>G=L HG M><AGH><HGHFB< :L
L>LLF>GM 1" H? LN@:K<:G> ;BHK>iG>KB>L BH?N>E />L '   
89 * *:G:G 4>;; *H=>KG FB<KH;B:E LHEB= LM:M> ?>KF>GM:MBHG M><AGHEH@R ?HK
?NMNK> ;BHK>iG>KB>L ?HK MA> IKH=N<MBHG H? :==>=O:EN> IKH=N<ML BH?N>E />L '
  
89 # A>KN;BGB 1A> ;BHK>iG>KR <HG<>IM NLBG@ ;BHF:LL BGLM>:= H? HBE ?HK IKH=N<BG@
>G>K@R :G= <A>FB<:EL "G>K@R HGO>KL *:G:@   
89 * 6:G@ ' 6NG % 7A:G@ 1 *:@H<A: % 7:;>= 6 5N> >M :E $>G>MB<:EER
>G@BG>>K>= LMK:BGL :IIEB<:MBHG :G= :=O:G<>L ?HK  IKHI:G>=BHE IKH=N<MBHG
?KHF @ER<>KHE #HH= 1><AGHE BHM><AGHE   
89 . $NH 6 G ' 6NG * 6:G@ 1 *:@H<A: ' 7AN >M :E "GA:G<>=
qM:@:MHL> IKH=N<MBHG ;R LIHK> LNK?:<>=BLIE:R>= r:K:;BGHL> BLHF>K:L> ?KHF BLH
E:M>= ):<MH;:<BEENL ;K>OBL -  :G= ;BHMK:GL?HKF:MBHG BHK>LHNK 1><AGHE
  
89 . $NH % 7:;>= % 7A:G@ 5 4:G@ ' 6NG $ 7A:G@ >M :E ,IMBFBS:MBHG H?
?>KF>GM:MBHG F>=BNF ?HK : G>PER BLHE:M>= R>:LM LMK:BG 7R@HL:<<A:KHFR<>L
KHNQBB '*  :G= >O:EN:MBHG H? ?:<MHKL :??><MBG@ ;BHLRGMA>LBL H? !:K:;BMHE
)41   
89 0 (P:D 6  -:KD '% 0>H BHLRGMA>LBL H? AR=KHQRIKHIBHGB< :<B= ?KHF
@ER<>KHE BG K><HF;BG:GM "L<A>KB<AB: <HEB >QIK>LLBG@ ):<MH;:<BEENL ;K>OBL =A:
:G= =A:/ @>G> <ENLM>KL :G= " <HEB ( :E=% BHK>LHNK 1><AGHE  

89 % 7A:G@ ' 6NG % 7:;>= * 6:G@ $ 7A:G@ 6 .B >M :E -KH=N<MBHG H? QREBMHE
;R >QIK>LLBG@ QREBMHE =>AR=KH@>G:L> :G= :E<HAHE =>AR=KH@>G:L> ?KHF $EN
<HGH;:<M>K MA:BE:G=B<NL :G= <H;BHMK:GL?HKF:MBHG H? PAHE> <>EEL BHK>LHNK 1><A
GHE   
89 / =>G %::G =:IMBG@ MA> R>:LM <HGLHEB=:M>= ;BHIKH<>LLBG@ I:K:=B@F ?HK ;BHK>
iG>KB>L BH?N>E />L '   
89 - *:CB=B:G * 1:;:M:;:>B * 7>BGHE:;>=BGB *- +:@ALA;:G=B 6 ABLMB *>M:
;HEB< >G@BG>>KBG@ H? FB<KHHK@:GBLFL ?HK ;BH?N>E IKH=N<MBHG />G>P 0NLM:BG "G
>K@R />O   
89 4 4N /4 !:OBL * 1K:G$R:Fi  (NH ( ):NMMB 0 *BA:EM<A>O: >M :E
A:K:<M>KBS:MBHG H? ?HNK >G=HIARMB< ?NG@B :L IHM>GMB:E <HGLHEB=:M>= ;BHIKH<>LLBG@
AHLML ?HK <HGO>KLBHG H? EB@GH<>EENEHL> BGMH :=O:G<>= ;BH?N>EL IIE *B<KH;BHE
BHM><AGHE   

... This reduction can be attributed to the degradation of fermentable sugars into inhibitors as the residence time increased (Fig. S1, cf. Electronic Supplementary Material, ESM) [27]. It is evident that pretreatment temperature had a greater impact on the efficient dissolution of cellulose and hemicellulose in the LHW liquid compared to pretreatment time. ...
Article
In the present study, a sustainable pretreatment methodology combining liquid hot water and deep eutectic solvent is proposed for the efficient fractionation of hemicellulose, cellulose, and lignin from sugarcane bagasse, thereby facilitating the comprehensive utilization of both C5 and C6 sugars. The application of this combined pretreatment strategy to sugarcane bagasse led to notable enhancements in enzymatic saccharification and subsequent fermentation. Experiment results demonstrate that liquid hot water-deep eutectic solvent pretreatment yielded 85.05 ± 0.66 g·L−1 of total fermentable sugar (glucose: 60.96 ± 0.21 g·L−1, xylose: 24.09 ± 0.87 g·L−1) through enzymatic saccharification of sugarcane bagasse. Furthermore, fermentation of the pretreated sugarcane bagasse hydrolysate yielded 34.33 ± 3.15 g·L−1 of bioethanol. These findings confirm the effectiveness of liquid hot water-deep eutectic solvent pretreatment in separating lignocellulosic components, thus presenting a sustainable and promising pretreatment method for maximizing the valuable utilization of biomass resources.
... Various enzymes are used for dark fermentation. Cellobiohydrolase, hemicellulase, lysozymes, pectinases, chitinases are capable of effectively hydrolyzing the complex carbohydrates, whereas enzymes such as proteases and lipases tend to decompose proteins and lipids (Zabed et al. 2019). Special enzymes are often used to hydrolyze food waste. ...
Chapter
Dark fermentative biohydrogen production from various organic wastes has attracted increased interest in recent years due to the possibility of obtaining a carbon-neutral energy carrier along with partial stabilization of organic matter. This chapter compiles essential information on substrates of various origins that are of key importance for dark fermentative biohydrogen productivity. Simple sugars, industrial crops rich in carbohydrates, agricultural, industrial waste and wastewater are considered as the feedstocks. In addition, the role of the biochemical composition and concentration of the substrate are discussed. The diversity of native bacteria and the predominant groups of microorganisms inhabiting considered substrates of dark fermentation is studied. A significant point for increasing the efficiency of the dark fermentation process is physical, chemical, biological, and combined pretreatment of the feedstock in order to make it more accessible for microorganisms. At the end of the chapter, the main points that should be addressed in further research are outlined.
... Over the years, several lignocellulosic biomass pretreatments have been proposed such as reactive extrusion, microwave, ultrasound, pyrolysis, liquid hot water (LHW), ammonia fiber explosion (AFEX), sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL), and biological pretreatments [5][6][7][8]. However, their results remain limited in terms of efficiency, economics, or scaling up [9,10]. It is difficult to remove a high percentage of lignin from lignocellulosic biomass in a single pretreatment step, hence the need to explore the combination of two (or even more) pretreatments becomes interesting [11]. ...
Article
Full-text available
Lignocellulosic biomasses, mainly forestry and agricultural residues, are inexpensive, available and attractive to reduce the dependence of the world on fossil fuels. However, before their processing in biorefineries, they must undergo a pre-treatment to allow access to the desired compounds of interest (cellulose, hemicellulose, or lignin). However, the pretreatment step significantly reduces and affects the profitability of the biorefinery process. Several pretreatment technologies have been developed so far. However, taken individually, these methods do not make lignocellulosic biomass a fully cost-effective input for biorefineries, hence the current trend to combine technologies. Extrusion is currently one of the most attractive technologies. It is relatively new and has been combined with many other methods to pretreat various types of biomasses with interesting benefits and results. This article provides a critical review of pretreatment combinations involving extrusion and discusses the challenges, solutions, and R&D needs for these combinations.
Article
In light of the excessive use of fossil fuels and their associated environmental effects, more stress has been devoted to using lignocellulosic-rich resources to produce sustainable biofuel. Among lignocellulosic-rich biomass, sugarcane bagasse is a major agro-waste byproduct of the sugarcane industry. Due to high cellulose and low ash content, it has excellent potential to produce bio-ethanol. Conventional methods used for pre-treatment of lignocellulosic waste need more process efficiency and consume a large ecological footprint. Several processes in industries are carried out at high NaCl concentrations, high temperatures, and in the presence of solvents. Therefore, the scientific community has focused on halophiles for bio-ethanol production. The present review describes the use of potential lignocellulolytic haloarchaea for efficient pre-treatment and hydrolysis of sugarcane bagasse to produce bio-ethanol. Haloarchaea can increase the economic feasibility of the process since they can thrive in high salt concentration, extreme pH, elevated temperature, and low water activity conditions. Further, this review summarizes the traditionally employed strategies for the pre-treatment and hydrolysis of sugarcane bagasse, along with the latest findings on using extremophilic microbes and extremozymes. Besides this, the review explores the functional role of haloarchaea and their plant polymer degrading enzymes in acting as biocatalysts in biorefinery under extreme conditions. The integrated approach using haloarchaea to produce bio-ethanol will be technically feasible and benefit society economically and environmentally.
Preprint
Full-text available
The study aimed to assess the lignolytic enzyme production in three white rot fungi (WRF) upon stimulation with metal ion (FeSO 4 .7H 2 O) and thereby develop an optimal fungal consortium for biopulping applications. Invitro wood decay experiments conducted with Eucalyptus and Bamboo demonstrated significant weight loss following inoculation with Trametes versicolor (TV), Ganoderma lucidum (GL), and Schizophyllum commune (SC). Over time (45–90 days), the weight loss gradually increased in all inoculations, ranging from 6.5–16.9% for Eucalyptus and 7.9–17.7% for Bamboo. Qualitative plate assays revealed TV’s positive ligninolytic potential in all tests, SC in three, and GL in only two tests. Secondary screening confirmed laccase, manganese peroxidase, and lignin peroxidase activities for all fungi, with SC consistently exhibiting higher activity. Fourier Transform Infrared Spectroscopy analysis of Eucalyptus sawdust treated with individual fungi and the consortium (TV, GL, and SC) showed significant lignin degradation compared to the control. While individual strains displayed a decrease in lignin-associated bands, the consortium exhibited a more pronounced decrease (98.55% and 84.21% at I 1511 /I 1739 and I 1248 /I 1739 ), indicating synergistic effects. Conversely, cellulose and hemicellulose associated band intensities increased, suggesting preferential lignin degradation by the fungal consortium. Overall, the study highlights the potential of all three fungal isolates to produce lignolytic enzymes with industrial applications.
Chapter
The concept of renewable carbon and its relevance in the context of sustainable economy and mitigation of environmental impacts caused by fossil fuel usage is essential. Carbon is a versatile chemical element, present in various molecules and widely utilized in the industry for fuel production, chemicals, polymer materials, energy, and others. However, the use of fossil carbon is limited and has adverse environmental impacts, such as the emission of carbon dioxide (CO2) responsible for global warming. In pursuit of alternatives, the concept of renewable carbon gains prominence, referring to resources that can be regenerated through the carbon cycle, such as CO2 and methane from natural or anthropogenic sources. Renewable carbon sources include microalgae biomass, lignocellulosic residues, the pulp and paper industry, as well as fruit and vegetable processing waste. The production of biofuels and high-value products from these sources forms the foundation of sustainable bioeconomy, which seeks to employ advanced technologies for efficient and circular utilization of natural resources. Sustainable bioeconomy is crucial to address the challenges posed by the growing global population and to ensure environmental preservation. Combining agricultural and forest biomass is a promising strategy to meet the demand for raw materials and expand clean energy and bioproducts production. However, the implementation of biorefineries and other renewable carbon technologies still faces challenges, such as the lack of detailed technical–economic assessments and adequate life cycle analyses.
Chapter
Bioactive metabolites are a diverse group of compounds with specific biological activities and are produced by various living organisms. Plants and microbes produces different bioactive molecules which plays an important role in biofuel production. Bioactive materials enhances the biofuel production by modulating different metabolic pathways. Several enzymes and organic acids produced by microbes helps in degrading the complex sugar to improve the fermentation process which helps in increasing the efficacy and yield of biofuel production. Phytocompounds such as terpenoids, are considered as precursor molecule for bioethanol and biodiesel generation. Additionally, these metabolites also possess antifungal and antibacterial properties, which reduces the contamination occurring during the process of biofuel generation. Several studies also reveal that these phytocompounds also helps in extraction and purification of biofuel. Hence bioactive compounds plays a crucial and multifaceted role in biofuel production. As sustainability and efficacy of biofuel production hold major importance, microbial and plant derived bioactive compounds can redefine the field of renewable energy.
Article
Protection of environment is of immediate concern and this can only be achieved by avoiding the use of chemicals for fuel production. Lignocellulosic waste is becoming popular as a feedstock for biofuel production. The can be converted into usable form for biofuel production by using a suitable pretreatment method. Different pretreatment methods have been used by researchers which are physical, chemical, physico-chemical, biological and combined pretreatments. Evidently chemical pretreatment is found to be more expensive as a large amount of chemicals are used for pretreating the lignocellulosic substrate. It has been shown that combined pretreatments are more effective as compared to single pretreatment and there is an extensive scope of combinations which can also be applied in future. Recent review critically discusses and compares different pretreatment methods, biomass resources, chemical composition of different agricultural biomass and the use of this biomass for bioenergy generation. Various pretreatment processes used for bio-hydrogen, bio-methane, bio-ethanol, bio-methanol bio-butanol and bio-diesel production are also discussed.
Article
Increasing energy demands are not only exploiting the fossil resources but, also depleting natural environment. Biofuels from lignocellulosic biomass is a renewable, ecofriendly, sustainable and could be a promising alternative to fossil fuels. However, pretreatment is an essential step to disarray the layers of lignocellulose prior to enzymatic hydrolysis. Among various pretreatments of lignocellulose, the biological pretreatment using microorganisms such as bacteria and fungi are gaining popularity due to its financial and environmental benefits. Careful selection of the suitable microbial consortium for efficient pretreatment of biomass is a critical step. The co-culture of bacteria and/or fungi in consolidated bioprocessing (CBP) is highly beneficial in the breakdown of complex biopolymers due to their high enzyme activity. Our selection of highly promising bacterial and/or fungal consortium has the ability to produce various extracellular enzymes including cellulase, hemicellulase, and lignases. It can be used in CBP for efficient biological pretreatment of lignocellulosic biomass following production of biofuels and bioproducts.
Article
In order to commercially exploit microalgae for the production of fucoxanthin, species must remain productive in the increasingly saline environment typical in outdoor cultivation ponds. To this end, this study investigated the salinity range, growth, fucoxanthin content and productivity of two halotolerant and four marine microalgae under salinity increase condition. The semi-continuous cultivation followed by gradual salinity increase and slow adaptation helped saline microalgae to extend the salinity range up to 55%. Tested species showed about 12% to 90% more fucoxanthin content at their optimal salinity compared to when grown at non-optimal salinities. Fucoxanthin productivity was found directly linked to biomass productivity. Marine microalgae performed best at salinities < 55‰ (ppt, parts per thousand) and halotolerant microalgae was best at salinities > 55‰. Among marine species, the highest fucoxanthin content and productivity was observed in Chaetoceros muelleri which was 2.92 mg g− 1 and 0.072 mg L− 1 d− 1 of ash free dry weight (AFDW), respectively, at 45‰ and fucoxanthin content was relatively consistent over the range of salinity between 35 and 55‰. Between two halotolerants, Amphora sp. showed the highest content and productivity of fucoxanthin which was 1.2 mg g− 1 and 0.053 mg L− 1 d− 1 of AFDW, respectively at 85‰ salinity. The results indicate that it is most likely possible to achieve continuous production of fucoxanthin by cultivating marine and halotolerant species one after another when salinity rises due to evaporation. Details of fucoxanthin content and productivity for Chrysotila carterae, Chaetoceros muelleri, Amphora sp. and Navicula sp. are reported for the first time.
Article
Metabolically engineered bacterial strains are fast becoming the center of attraction in the biosynthesis of various bio-based ingredients. Currently, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) with its associated RNA guided endonuclease (Cas9), is considered as the most promising tool that has brought a breakthrough in the genome editing approach. Although this versatile system originates from the immune systems of bacteria, its application in bacterial genome editing as an approach of metabolic engineering is still not so extensive. This review paper discusses the challenges, opportunities and application of CRISPR/Cas system in bacterial genome editing for biosynthesis of various value-added ingredients. The content of this paper mainly emphasizes Type II CRISPR/Cas9 system used for knock-in and knock-out of various target genes in the bacterial strains as a pre-requisite for increasing the production of bio-based materials. Keywords CRISPR/Cas9, Value-added ingredients, Bacterial genome editing, Strain improvement, Microbial biosynthesis
Article
A newly isolated yeast strain, namely Zygosaccharomyces rouxii JM-C46, was reported to be promising for enhanced D-arabitol production from glucose. In this study, further efforts were made to optimize fermentation conditions for this strain through one-factor-at-a-time strategy considering seven important factors of shake flask fermentation. Subsequently, composition of fermentation medium was optimized using Box-Behnken design (BBD) of response surface methodology (RSM) including three major nitrogen providing ingredients, namely yeast extract, (NH4)2SO4 and peptone. Maximum D-arabitol concentration produced in one-factor-at-a-time experiments was found to be 72.69 g/L under the optimum conditions consisting of 200 g/L initial glucose, 100 ml medium, 5% (equivalent to 8.78 g/L) initial inoculum, temperature 30 °C, pH 5.0, fermentation time 96 h, and agitation rate 200 rpm. In the experiments of BBD, the predicted yield of D-arabitol varied between 67.73 g/L and 76.76 g/L, while the actual concentration of D-arabitol ranged from 68.02 g/L to 76.95 g/L. The optimum composition of medium F (final medium) contained D-glucose (200 g/L), yeast extract (10 g/L), (NH4)2SO4 (2 g/L) and peptone (7.5 g/L), which further increased D-arabitol production from 72.69 g/L to 76.32 g/L.
Chapter
Generation of biofuels from lignocellulosic biomass has received much interest in recent times to achieve an alternative energy source over conventional fossil fuels. Pretreatment is a vital step in the bioconversion of lignocellulosic biomass into biofuels, which is required to break down the lignocellulosic network of biomass. It is necessarily applied prior to the production of bioalcohols (bioethanol and biobutanol), biohydrogen, and biogas through fermentation. Delignification is the main objective of pretreatment that releases polysaccharides from the lignocellulosic matrix and increases enzymatic digestibility of cellulose. Although pretreatment can be done by using different physical, chemical, physicochemical, and biological methods, the latter is considered more promising as it is less expensive and eco-friendly, generates low or no inhibitors, and consumes relatively lower energy (steam and electricity). Many naturally occurring ligninolytic microorganisms and enzymes are used for delignification of biomass biologically. The aim of this chapter is to present an overview of different ligninolytic microorganisms (fungi and bacteria) and their enzymes for biological pretreatment of lignocellulosic biomass.
Article
Continuous exploitation of natural resources especially in fossil fuels to fulfil the market demand has jeopardized the natural resources in the Earth. Bioethanol produced by microalgae is one of the promising biofuel for energy security and ecological sustainability. Yet, to date only a modest review has been reported on the bioethanol production technology from microalgae by using various pretreatment methods, fermentative microorganisms, fermentation processes, commercialization techniques and its environmental perspectives. This review paper aims to provide a comprehensive information by comparing the recent pretreatment technologies by using different types of ethanologenic microorganisms in various fermentation processes to maximize the bioethanol production. Studies of the economic viability and environmental perspectives of bioethanol from microalgae have been carried out to investigate its potentialities to eliminate environmental issues and towards commercialization. Therefore, this review is essential in providing ideas for the future researches in renewable energy resources technology to develop a more efficient way to scale-up the bioethanol production from microalgae for commercial and industrial application.
Article
Pretreatment of lignocellulosic biomass to overcome its intrinsic recalcitrant nature prior to the production of valuable chemicals has been studied for nearly 200 years. Research has targeted eco-friendly, economical and time-effective solutions, together with a simplified large-scale operational approach. Commonly used pretreatment methods, such as chemical, physico-chemical and biological techniques are still insufficient to meet optimal industrial production requirements in a sustainable way. Recently, advances in applied chemistry approaches conducted under extreme and non-classical conditions has led to possible commercial solutions in the marketplace (e.g. High hydrostatic pressure, High pressure homogenizer, Microwave, Ultrasound technologies). These new industrial technologies are promising candidates as sustainable green pretreatment solutions for lignocellulosic biomass utilization in a large scale biorefinery. This article reviews the application of selected emerging technologies such as ionizing and non-ionizing radiation, pulsed electrical field, ultrasound and high pressure as promising technologies in the valorization of lignocellulosic biomass.