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Publications (105)
Background:
The purpose of the study was to evaluate lipid homeostasis before and after treatment of everolimus, the mammalian target of the rapamycin (mTOR) inhibitor, among patients with tuberous sclerosis complex (TSC).
Methods:
The study group consisted of 15 patients with a diagnosis of subependymal giant cell astrocytoma (SEGA) related to...
The aim: of the study was to investigate clinical and genetic determinants of arterial stiffness in children and adolescents with type 1 diabetes mellitus.
Material and Methods: 122 patients (mean age: 16.0±2.35 years), with an average diabetes duration of 5.0 years and without evidence of arterial hypertension were recruited. Ambulatory arterial s...
Introduction:
It remains unclear how HbA1c recommendations influence metabolic control of paediatric patients with type 1 diabetes mellitus. To evaluate this we compared reported HbA1c with guideline thresholds.
Materials and Methods:
We searched systematically MEDLINE and EMBASE for studies reporting on HbA1c in children with T1DM and grouped th...
Purpose:
The aim of this study was to compare the incidence of dental complications in childhood cancer survivors with that of healthy controls subjects, and to determine the possible influence of various factors associated with patient and treatment.
Materials and methods:
Sixty-one panoramic radiographs of the dentition of cancer survivors wer...
Subependymal giant cell astrocytoma (SEGA) is a benign brain tumor associated with tuberous sclerosis complex (TSC). Since there were some discrepancies in SEGA classification, in 2012, a consensus statement defined SEGA as a lesion at the caudothalamic groove with either a size of more than 1. cm in any direction or a subependymal lesion at any lo...
Background:
The role of T regulatory lymphocytes has been investigated in various allergic diseases. However, the precise relation between the phenotype and severity of allergic diseases and the changes in FOXP3 mRNA expression are not fully understood.
Objective:
To compare the expression of FOXP3 mRNA in children with asthma with and without c...
Background:
Essential oils were proven to possess analgesic activity in adults. Children with diabetes are exposed to highly painful interventions such as self-monitoring of blood glucose (SMBG).
Objective:
An evaluation of the analgesic properties of two essential oils during SMBG in diabetic children.
Subjects:
We included 73 hospitalized ch...
Congenital hyperinsulinism of Infancy (CHI) comprises heterogenic defects of insulin secretion with diverse molecular aetiology, histological features, severity of symptoms, and response to pharmacotherapy. The study aimed to establish the first clinical characteristics of Polish patients with CHI and to propose a novel clinical algorithm allowing...
BACKGROUND
We investigated whether in young children with inadequately controlled type 1 diabetes and technical problems with continuous subcutaneous infusion of insulin at 100 units/mL the switch to insulin diluted to 10 units/mL (U10) can limit technical problems and improve glycemic control.
SUBJECTS AND METHODS:
Diluted U10 insulin was started...
Hepatocyte transforming factor 1B-maturity onset diabetes mellitus of the young (HNF1B-MODY) is an autosomal dominant type of monogenic diabetes caused by a mutation in the gene encoding hepatocyte nuclear factor 1beta (HNF-1beta). The aim of this study was to determine if a HNF1B gene mutation was responsible for a dominantly inherited form of dia...
The role of HLA-G is extensively studied in cancer due to its inhibition of the immune response. Several polymorphisms in the HLA-G gene have been reported to significantly affect its expression. We, therefore, investigated whether functionally relevant HLA-G polymorphisms, HLA-G-725C/G/T, and HLA-G 14-base pair, have any influence on the susceptib...
To analyze the incidence and outcome of infections with Staphylococcus spp. in children with malignancies treated in pediatric hematology and oncology (PHO) centers or undergoing hematopoietic stem cell transplantation (HSCT) over a period of 24 months.
Immunosuppressive therapy is the treatment of choice in children with acquired severe aplastic anemia (AA) and no HLA-matched family donor. The paper presents results of a multicenter study of 63 children with AA treated with rabbit antithymocyte globulin (r-ATG) and cyclosporine A as the first line treatment in the years 1996-2012. Therapeutic eff...
RAB40AL has been reported as the locus for Martin-Probst syndrome (MPS), an X-linked deafness-intellectual disability syndrome. The report was based on segregation of a missense change p.D59G with the disease in a single family and in vitro localization studies. We found the p.D59G variant by whole exome sequencing in two patients, however the diag...
Aim:
To investigate whether autoimmune thyroiditis (AIT) in children with type 1 diabetes mellitus (DM1) has any influence on glycemic control, lipid profile or thyroid volume.
Methods:
A total of 330 patients with DM1 and AIT (DM1+AIT group) were compared with 309 children with DM1 without AIT (control group). Patients were treated in four Poli...
Introduction:
Wolfram syndrome (WFS) is the most frequent syndromic form of monogenic diabetes coexisting with optic atrophy and many other disorders. The aim of this study was to estimate the prevalence of Wolfram syndrome among children with diabetes in Poland.
Material and methods:
These calculations were performed among Polish diabetic child...
The objective was to compare the impact of clinical and genetic factors on body mass index (BMI) in children with type 1 diabetes (T1DM) without severe obesity. A total of 1,119 children with T1DM (aged 4-18 years) were qualified to take part in the study. All children were genotyped for variants of FTO, MC4R, INSIG2, FASN, NPC1, PTER, SIRT1, MAF,...
Nijmegen breakage syndrome (NBS) is an autosomal recessive chromosomal instability disorder characterized by a high incidence of pediatric hematologic malignancies. Majority of patients affected are of Slavic origin and share the same founder mutation of 657del5 within the NBN gene encoding protein involved in DNA double-strand breaks (DSB) repair....
Introduction:
Sustained inflammation in sarcoidosis may lead to lung fibrosis. The activity of numerous chemokines responsible for proliferation and activity of T lymphocytes may play a crucial role in this process and may have predictive value. These include cytokines induced by interferon γ, such as CXCL9, 10, and 11-ligands of chemokine recepto...
The significance of lymph node metastases and the optimal extent of lymphadenectomy remain matters of controversy in papillary thyroid cancer. This study was designed to assess the feasibility and reliability of OSNA and real-time PCR for CK19 and TG mRNA in papillary thyroid cancer lymph nodes evaluation compared to standard histopathology.
Each o...
Novel markers of nephrotoxicity, including kidney injury molecule 1 (KIM-1), interleukin 18 (IL-18), and beta-2 microglobulin, were used in the detection of acute renal injury. The aim of the study was to establish the frequency of postchemotherapy chronic kidney dysfunction in children and to assess the efficacy of IL-18, KIM-1, and beta-2 microgl...
Background:
The presented method is able to determine 6-mercaptopurine (6-MP), 6-thioguanine, 6-mercaptopurine riboside and 6-thioguanine riboside in urine, and is thereby dedicated to control of thiopurine therapy of children with acute lymphoblastic leukemia. Good separation of the mentioned compounds was achieved on a C18 stationary phase with...
Background:
The E23K variant of the KCNJ11 gene is possibly responsible for changes in insulin secretion during the fetal life. We tried to assess the influence of the E23K variant on birth weight and metabolic profile in prepubertal children born small for gestational age (SGA).
Subjects:
One hundred and twenty-three SGA and 132 born appropriat...
Unlabelled:
Genetic and environmental factors have an influence on the process of growth and development of the body. One of numerous genetic factors can be the vitamin D receptor gene (VDR). The study aimed at evaluating the relationship between VDR polymorphism and somatic parameters in children.
Patients and methods:
The study group consisted...
Despite the progress in diagnosis and treatment of type 1 diabetes, diabetic ketoacidosis (DKA) is still a serious clinical problem. The aim of the study was too describe the epidemiology and clinical characteristic of DKA in children and adolescents with type 1 diabetes.
Medical records of 650 patients with type 1 diabetes who were under care of t...
To compare the diagnostic accuracy and cost-effectiveness of screening models based on glycated hemoglobin level (HbA1c) and psychometric measures in detecting mood disorders among children with type 1 diabetes (T1DM)MethodsA random sample of 26% out of 632 available subjects aged 8–18 years with T1DM lasting longer than 12 months were enrolled to...
To evaluate the impact on mortality and duration of stay of weekend admission of paediatric patients to the Paediatric/Neonatal Intensive Care Unit (PNICU).
Retrospective, nine-year cohort study. The study was performed in a tertiary level PNICU between 1 January 1999 and 31 December 2007. Data about the day of admission, diagnosis, outcome, and du...
Bone fractures may depend on Vitamin D Receptor Gene (VDR), bone mineral density, bone turnover markers. Patients and methods. 161 patients were recruited and underwent: skeletal densitometry (DXA) method and bone turnover studies (Osteocalcin and Ntx).The study group was evaluated using restriction enzyme digestion at BsmI (rs1544410), FokI (rs222...
Introduction:
The laryngeal cancer is the most common malignancy of the head and neck squamous cell carcinoma. Recent studies have revealed the role of genetic variations in the risk for laryngeal cancer. Polymorphic genes of matrix metalloproteinases may affect an individual genetic predisposition to the occurrence and clinical implications of th...
In pediatric patients with type 1 diabetes mellitus, the value of HbA1c is a predictor of the risk of late systemic complications in adulthood. In the last years significant changes in the method of treatment in pre-pubertal children with T1DM have taken place. However, there is lack of precise data concerning the results of metabolic control of th...
Introduction:
Glycated haemoglobin is currently a golden standard of evaluation of metabolic control of diabetes. According to the Evidence based Medicine data, better metabolic control of diabetes decreases the prevalence of chronic complications.
Aim of the study:
to analyse HbA1c in children with a longer duration of type 1 diabetes mellitus...
Introduction:
collection of family history of diabetes mellitus (DM) is commonly performed when this illness is diagnosed in children. However the significance of gleaned information may differ depending on the affected family members.
Aim of the study:
this study was performed in order to describe detailed familial history of DM in patients and...
Human leukocyte antigens (HLA) complex and INS-IGF2 5'VNTR loci are principal determinants of the risk of type 1 diabetes mellitus (T1DM). Carriage of class III allele is protective, while class I/I homozygosity increases the risk of T1DM.
HLA and 5'VNTR allele frequencies were summarised and multivariate logistic regression models with interaction...
Recent studies have shown that SNPs mapping to 7p12.2 (IKZF1), 9p21 (CDKN2A), 10q21.2 (ARID5B), and 14q11.2 (CEBPE) and carrier status for recessively inherited Nijmegen Breakage syndrome (NBS) influence childhood acute lymphoblastic leukemia (ALL) risk. To examine these relationship, we analysed 398 ALL cases and 731 controls from Poland. Statisti...
To determine (i) whether insulin preparations produced by three companies induce the same immune responses in insulin-naïve children with type 1 diabetes (T1DM); (ii) if switching from human insulin to rapid-acting insulin analogs influences this immune response; and (iii) if different insulin brands produce different clinical results during the fi...
Behavioral problems are an issue among adolescents with type 1 diabetes (T1D). The authors hypothesize that theft, possibly because of an underlying psychiatric morbidity, may be a way of procuring sweets leading to worse glycemic control.
To evaluate psychiatric morbidity and the association of theft and metabolic control among children and adoles...
Purpose:
Antibacterial immunity in diabetes is impaired, which increases the risk of general and local infections. The aim of the study was to evaluate non-specific local antibacterial immunity based on lactoferrin and lysozyme concentration in tears in children with diabetes type 1.
Material and methods:
Children at the age of 10-18 years old w...
Diabetes mellitus may occur in children and adolescents as an independent disease, most frequently as autoimmune type 1 diabetes, or can coexist with other abnormalities. If diabetes coincides with other disorders occurring sequentially, a syndromic form of monogenic diabetes should be suspected. Thiamine-responsive megaloblastic anaemia (TRMA) syn...
The most frequent type of diabetes in childhood is type 1 diabetes. Thanks to the development of genetic testing, the rare monogenic forms of that disease have been defined. One of them is neonatal diabetes identified within the first 6 months of life and often associated with the mutation in KCNJ11, ABCC8 or insulin gene. A less frequent mutation...
Chemokines may modulate autoimmunity through a variety of mechanisms. Recent reports suggest involvement of the IP10 (CXCL10) chemokine in autoimmunity towards pancreatic β cells.
The purpose of this study was to evaluate the effects of genetic variability of IP10 and its dependence on HLA-conferred risk of diabetes.
148 children with type 1 diabet...
The glycated A1c hemoglobin (HbA(1)c) is an accepted marker of glycemic control in patients with type 1 diabetes (T1DM) and a predictor of long-term microvascular diabetic complications. There have been reports of seasonal fluctuations of HbA(1)c levels in type 1 and type 2 diabetic patients of different age.
The aim of the study was to investigate...
Recent studies on the pathogenesis of type 1 diabetes (T1DM) show that autoimmune activity in human pancreatic 1-cells is accompanied by abnormalities of fatty tissue metabolism, which is the underlying process in type 2 diabetes.The aim of the study was to determine the correlation between C-peptide concentration, indicating residual insulin secre...
Proinsulin 5'VNTR polymorphism determines susceptibility to type 1 diabetes (T1DM). The authors tested whether it affects intravenous glucose tolerance test (IVGTT) results. Aim of the study: To evaluate a possible relationship between 5'VNTR proinsulin gene (INS) polymorphism and glucose, insulin and C-peptide levels during IVGTT among siblings of...
Self-monitoring of blood glucose (SMBG) is one of the cornerstones of diabetes management.
To evaluate the potential for miscoding of a personal glucometer, to define a target population among pediatric patients with diabetes for a non-coding glucometer and the accuracy of the Contour TS non-coding system.
Potential for miscoding during self-monito...
In order to assess if any differences exist in children germ cell tumours depending on age, we compared some features of germ cell tumours in two age groups:younger than 10 and between 11 and 18 years.
Data of 146 patients with germ cell tumours treated in 15 Polish paediatric oncology departments between 1995 and 2005 were evaluated. They were div...
Very few recent Polish data are available on the epidemiology of Chlamydophil pneumoniae (C. pneumoniae) as causative agent among paediatric patients, hospitalized with respiratory tract disorders. Extending these data would serve as rationale for empirical antimicrobial therapy. The aim of the study was to evaluate the frequency of C. pneumoniae i...
Objectives
To determine the influence of parent and child depressive and anxiety symptoms, family functioning and its influence on child quality of life and diabetes control among children and adolescents.
Methods
149 Children and adolescents (aged 8-18 years) with duration of diabetes longer than 1 year were enrolled to the study. Participants an...
Pemphigus is an autoimmune blistering disease mediated by circulating IgG autoantibodies directed against desmogleins 3 and/or 1. As pemphigus is a T cell-mediated disease, one may assume that genetically determined disregulation of costimulatory signal may be involved in its pathogenesis.
The aim of the present study was to evaluate the relationsh...
Regulatory T cells (Tregs) have an essential role in tolerance and immune regulation. However, few and controversial data have been published to date on the role and number of these cells in food allergic children. The forkhead/winged-helix transcription factor box protein 3 (FOXP3) is considered the most reliable marker for Tregs.
This study aims...
The pathogenesis of type 1 diabetes is connected with immune-mediated beta-cell destruction leading to insulin deficiency. The majority of patients will become completely incapable of insulin secretion within a few years, however, some individuals will have persistent beta-cell function years after the diagnosis of diabetes. Despite clinical sympto...
Due to the lack of precise diagnostic criteria, current search strategy for monogenic diabetes is predominantly based on atypical clinical course of diabetes and intuition of the attending physician. Yet another issue is the common view that monogenic diabetes is rare. It discourages from performing deepened diagnostics and makes it difficult to ga...
Cicatrisation of the renal cortex is closely related to chronic infections of the urinary system. Static renal scintigraphy is used as the method enabling detection of local defects of radiopharmaceutical uptake, and is treated as the "gold standard" in the diagnosis of renal scars. The aim of the reported investigation was a comparison of the diag...
The aim of the paper is to present the initial results of molecular examination which was started in 2006 for children with acute myeloid leukemia. Better knowledge of biology of this disease, can result in establishing of new risk factors what allows more precise patient stratification to different therapeutic groups. Study was obtained patients u...
Currently over 90% of children and adolescents with Hodgkin's disease (HD) can be cured thanks to use of multidrug chemotherapy (CT) combined with involved-field radiotherapy (IF-RT). However, the intensive treatment may increase the risk of late complications which may impair the patients' quality of life. In order to decrease the incidence of lat...
Approximately 60 children aged 0-18 years are diagnosed of NBL each year in Poland. About 60% of all patients suffering from NBL have a chance for durable cure. Unfortunately the prognosis for patients within the high-risk group accounting for more than 50% of all NBL patients remains poor despite the introduction of more intensive chemotherapy reg...
A clinical criterion of the Wolfram syndrome is the coexistence of diabetes and optic atrophy recognized before the age of 15. Diabetes present in Wolfram syndrome is a result of the selective β cell loss and failed insulin secretion which is probably associated with non-autoimmune pathogenesis.
The aim of the study was an evaluation of HLA subtype...
In children born small for gestational age (SGA), some of the metabolic syndrome (MS) components are observed. The goal of the study was an evaluation of the incidence of particular components of MS in 5-9-years-old SGA children.
Ninety-one prepubertal SGA children (34 boys and 57 girls) were qualified into the study, aged 4.78-9.75 yrs (6.9±1.37 y...
The current paper presents a case of 14 months old girl with WAGR's syndrome. This syndrome is a genetic disorder characterized by the deletion at 11p13 locus which gives clinical presentation of aniridia, Wilms' tumor, genitourinary anomalies and mental retardation. Although WAGR's syndrome is a rare disorder, knowledge of its presentation is help...
Repeated administration of l-asparaginase leads to the development of specific antibodies and hypersensitivity reactions. The aim of the study was to evaluate a possible cross-reaction of anti-asparaginase antibodies, developed against the native E. coli
l-asparaginase (Asparaginase Medac), with other preparations of the enzyme. Sixteen patients wi...
The rapid rising prevalence of childhood obesity is related to the increased risk of cardiovascular morbidities. The type 1 diabetic patients are the group at special risk of macroangiopathy. The aim of the study was to estimate the prevalence of abdominal obesity in type 1 diabetic children compared to the general population, and to estimate the p...
Prevalence of arterial hypertension and prehypertension in children and adolescents with type 1 diabetes mellitus is higher than in healthy people, even when diabetic nephropathy symptoms (microalbuminuria) in these patients are not detected.
To estimate the influence of body mass and body composition on blood pressure in children and adolescents w...
Loss of circadian blood pressure (BP) variation (i.e., lack of nocturnal BP dip by at least 10mmHg, 'non-dipping') is associated with increased mortality rate in subjects with diabetes. We studied whether angiotensin converting enzyme (ACE) insertion/deletion (I/D) gene polymorphism may play a role in 24-h BP rhythm control.
The study group was 38...
To estimate insulin sensitivity in Type 1 diabetic children and adolescents, and assess the relationship between insulin sensitivity and clinical markers of adiposity and parameters of the metabolic syndrome.
A total of 202 patients aged 8-18 years with Type 1 diabetes and disease duration 1.5-15 years participated. Insulin sensitivity was estimate...
Unlabelled:
The aim of the study was a prospective evaluation of the lipid profile in children with acute lymphoblastic leukaemia, before and during the treatment with L-asparaginase.
Patients and methods:
Twenty-four children treated according to the ALLIC 2002 protocol entered the study. Blood samples were collected during the induction phase...
Bone marrow transplantation from HLA identical family donors is the treatment of choice for children with severe aplastic anaemia (SAA). When there is no donor available, combined immunosuppressive therapy is given.
evaluation of results of immunosupressive therapy in children with severe aplastic anaemia. Material and methods: SAA was diagnosed in...
According to the WHO classification, type 1 diabetes is divided into type 1A - autoimmune and type 1B - idiopathic. The pathogenesis of the latter remains unknown. Clinical observations confirm that type 1 diabetes is often associated with the presence of other organ-specific autoantibodies. Besides, the idiopathic type 1 diabetes is suggested as r...
Based on etiology a new classification for diabetes mellitus was proposed by the Experts of WHO in 1999. According to the classification, type 1 diabetes was subclassified as type 1A (autoimmune) and type 1B (idiopathic). Both forms result from the destruction of beta cells, however in type 1A the process is immune-mediated but in type 1B the origi...
The most frequent form of diabetes in the childhood is type 1 diabetes. Moreover, the rare forms of diabetes have been also identified in children. Besides of neonatal diabetes caused by the mutations in KCNJ11, SUR1 and GCK genes, other forms of monogenic diabetes are associated with different chronic disorders. These rare forms of syndromic diabe...
Diabetes, which is diagnosed before 6 months of age, is patogenetically different than type 1 diabetes. This kind of diabetes also known as a neonatal diabetes is genetically determined with monogenic mode of inheritance. Most of these patients are carriers of heterozygous mutation in the KCNJ11 or ABCC8 gene. These mutations may activate the Kir6....
The aims of the study were: 1. to evaluate the levels of insulin antibodies (IA) during the first 2 years of type 1 diabetes in children and adolescents, 2. to analyze relations between IA and several clinical and metabolic markers. Material and methods: Fifty seven patients (37 males and 20 females) aged 7.6-17.9 years (mean 13.2±2.4) participated...
The direct involvement of the human leukocyte antigen class II DR-DQ genes in type 1 diabetes (T1D) is well established, and these genes display a complex hierarchy of risk effects at the genotype and haplotype levels. We investigated, using data from 38 studies, whether the DR-DQ haplotypes and genotypes show the same relative predispositional eff...
The primary aim of the study was to evaluate the importance of anti-asparaginase antibodies for l-asparaginase activity in children with standard and medium risk acute lymphoblastic leukemia (ALL). Forty-seven children with newly diagnosed ALL were included into the prospective study. Enzyme activity and the presence of anti-asparaginase antibodies...
of this study was the evaluation of some factors having an influence on post-exercise albuminuria level in children with newly diagnosed type 1 diabetes as a prognostic factor of developing nephropathy.
24 newly diagnosed type 1 diabetic children, aged 5.5-16.9 years, mean 10.2+/-3.2, were examined. In order to provoke albuminuria the patients unde...
was to estimate the relationship between serum leptin levels and insulin sensitivity and components of metabolic syndrome in type 1 diabetic children and adolescents.
158 patients with type 1 diabetes mellitus (70 girls and 88 boys) aged from 8.2 to 18.4 years (mean: 14.1+/-3.1 years) were included into the study. The diabetes duration ranged from...
Proinsulin connecting peptide (C-peptide) has been initially regarded as deprived of biological functions other than correct scaffolding of insulin. This was caused by the lack of evident effect of C-peptide administration to healthy subjects or animals. At present, in view of numerous studies concerning its structure, membrane binding and biologic...
The aim of the study was to identify individual, clinical and metabolic factors that predict the C-peptide levels and appearance of clinical partial remission during the first year of type 1 diabetes (T1D) in children.
197 type 1 diabetic patients (84 female and 113 male, mean age 10.4 years) were examined. C-peptide levels were detected by radioim...
To evaluate gastric myoelectrical activity in children with newly diagnosed type 1 diabetes melliltus (T1DM) in relation to blood glucose control and visceral neuropathy.
Percutaneous electrogastrograpy (EGG) was performed on 42 children (20 F; mean age 12.9 +/- 3.1 years) with T1DM of <1 year's duration and on 35 healthy controls (18 F; mean age 1...
The aim of the study was to evaluate an association between ACE genotypes and blood pressure (BP) disturbances in children and adolescents withType 1 diabetes mellitus.
126 normo-albuminuric, type 1 diabetic children and adolescents at the age 10.5-19.7 years, and duration of diabetes 2.0-17.8 years, were included in the study. All patients were cl...
The influence of amifostine alone and in combination with doxorubicin, cytarabine, and etoposide on the cell growth and on bcl-2, bax and p65 gene expression was investigated in human acute promyelocytic leukemia cell line HL-60. No or very little influence of the exposure of HL-60 cells to amifostine (10(-6) to 10(-2) M) on cell proliferation was...
The aim of the study was to estimate the prevalence of metabolic syndrome in type I diabetic children and adolescents. Material and methods. 173 patients with type 1 diabetes mellitus (96 males) aged from 12 to 19.3 years (mean: 16.0±2.1) were included into the study. The diabetes duration ranged from 0.5 to 17.5 years (mean: 4.2±3.6 years). The he...
The significance of genetic background in childhood acute lymphoblastic leukemia (ALL) is not well understood. Polymorphisms of genes encoding for xenobiotics and drug transporters are potential factors, which can influence the risk of developing ALL and its clinical outcome. P-glycoprotein (P-gp) is an adenosine triphosphate-binding cassette (ABC)...
C-peptide level is the most reliable factor evaluating the endogenous insulin secretion in patients with type 1 diabetes.
The aim of the study was to investigate whether the age at onset, gender, presence of autoantibodies and ketoacidosis at diagnosis and insulin requirement, HbA1c levels could be applied to predict the C-peptide levels in the fir...
The significance of genetic background in childhood acute lymphoblastic leukemia (ALL) is not well understood. Polymorphisms of genes encoding for xenobiotic and drug transporters are potential factors, which can influence the risk of developing ALL and its clinical outcome. P-glycoprotein (P-gp) is an ABC-family transporter involved in protection...
The HLA complex, located on the short arm of chromosome 6, is the strongest genetic marker for type 1 diabetes (T1DM). In previous study we demonstrated association between genes HLA-DRB1 and HLA-DQB1 and T1DM in the Polish population. There is a strong-independent association of alleles HLA-DRB1*0401 and DQB1*302, despite population linkage disequ...
Patients with autoimmune type 1 diabetes mellitus have often, besides immune diabetes markers, also other organ-specific antibodies, particularly thyroid autoantibodies (antithyreoglobulin antibodies - ATG and/or thyroid peroxidase antibodies - TPO). In many of these patients autoimmune thyroid diseases, i.e. Hashimoto thyroiditis and Grave's disea...
To investigate angiotensin-converting enzyme (ACE) gene deletion/insertion (D/I) polymorphism as a risk factor of renal scarring in children, we determined ACE genotypes in 63 paediatric patients with primary vesicoureteric reflux (VUR) grade I-V and recurrent urinary tract infections (UTI). Renal cortical scars were evaluated applying Technetium-9...
The influence of carboplatin alone and carboplatin in combination with cytoprotective agent amifostine on the growth, caspase 3 activity and some apoptotic genes expression was investigated in vitro in human acute promyelocytic leukemia HL-60 cells. Proliferation of HL-60 cells exposed to carboplatin dropped down with increasing dose of the drug. T...
Clinical symptoms of type 1 diabetes are preceded by a long period of prediabetes stage characterised by anti-islet antibodies occurrence as well as insulin and C-peptide secretion disturbances. The aim of this study was to define the prognostic value of type 1 diabetes antiislet humoral markers (ICA, anti-GAD, anti-IA2 and IAA) and to find out thr...
In some patients the ketoacidosis at the onset of type 1 diabetes has been observed.
The aim of this study was to investigate an effect of the clinical, genetic, immunological and metabolic parameters on the occurrence of ketoacidosis at the clinical onset of the disease.
106 children with type 1 diabetes, aged 1.8-18.2 years (average 10.6), 40 fem...
Human lymphocytes, p53 protein-deficient acute promyelocytic leukemia cell line HL-60, murine pro-B lymphoid cell line BaF3 and its TEL/ABL-transformed clone cells were exposed to idarubicin with and without pre-treatment with amifostine. Idarubicin at 0.5-5 microM evoked DNA damage measured by the Comet assay. Amifostine at 14 mM decreased DNA-dam...
The infections occurring during the cancer treatment are severe clinical problems. The aim of the study was to estimate the changes occurring in the bacteriological profile of infections among children undergoing chemotherapy and changes in antibiotic-sensitivity. A retrospective analysis of 218 cases of infections that occurred in 105 children age...
The study aimed to verify the hypothesis that some alleles of HLA-DRB 1, -DQA 1, -DQB 1 genes may increase the risk for familial clustering of type 1 diabetes (T1D). We analysed 507 patients with T1D derived from simplex (S) and multiplex (M) families. PCR method followed by hybridisation with SSO probes was performed to genotype of HLA-DRB1, DQA1,...
The study aimed to evaluate fasting proinsulin levels in sibilings of type 1 diabetes patients with different risk for developing the disease (n=30) and children with type 1 diabetes (n=115). We studied fasting proinsulin (PRO), insulin (INS) and C-peptide (P-C) levels by radioimmunoassay. Moreover, the PRO/INS, PRO/P-C ratios were calculated. High...
Idarubicin is an anthracycline anticancer drug used in haematological malignancies. The main side effect of idarubicin is free-radicals based cardiotoxicity. Using the comet assay we showed that the drug at concentrations from the range 0.001 to 10 microM induced DNA damage in normal human lymphocytes, measured as the increase in percentage of DNA...
