Tatsuya Mishima

Kyorin University · Department of Medical Physiology

Ultrasound is highly biopermeable and can non-invasively penetrate deep into the brain. Stimulation with patterned low-intensity ultrasound can induce sustained inhibition of neural activity in humans and animals, with potential implications for research and therapeutics. Although mechanosensitive channels are involved, the cellular and molecular m...

De novo heterozygous mutations in the STX1B gene, encoding syntaxin 1B, cause a familial, fever‐associated epilepsy syndrome. Syntaxin 1B is an essential component of the pre‐synaptic neurotransmitter release machinery as a soluble N‐ethylmaleimide‐sensitive factor attachment protein receptor protein that regulates the exocytosis of synaptic vesicl...

In neuronal plasma membrane, two syntaxin isoforms, HPC-1/syntaxin1A (STX1A) and syntaxin1B (STX1B), are predominantly expressed as soluble N-ethylmaleimide-sensitive fusion attachment protein receptors, also known as t-SNAREs. We previously reported that glutamatergic and GABAergic synaptic transmission is impaired in Stx1b null mutant (Stx1b−/−)...

Supplementary material

This paper reports the data for the effects of organelle stresses on the ER-Golgi-soluble N-ethylmaleimide-sensitive factor-attachment protein receptors (ER-Golgi SNAREs) syntaxin 5 (Syx5) in neuronal cells. Quantitative as well as qualitative data are presented here to verify the upregulation of Syntaxin 5 (Syx5) under ER and Golgi stresses in hip...

Unresolved endoplasmic reticulum (ER) stress causes neuronal death and has been implicated in neurodegenerative conditions such as Alzheimer's disease (AD). However, the mechanisms by which stress signals propagate from the ER through the Golgi apparatus and their effects on the transport and processing of AD-related proteins, such as β-amyloid pre...

Two types of syntaxin 1 isoforms, HPC ‐1/syntaxin 1A ( STX 1A) and syntaxin 1B ( STX 1B), are thought to have similar functions in exocytosis of synaptic vesicles. STX 1A −/− mice which we generated previously develop normally, possibly because of compensation by STX 1B. We produced STX 1B −/− mice using targeted gene disruption and investigated th...

Two syntaxin 1 (STX1) isoforms, HPC-1/STX1A and STX1B, are coexpressed in neurons and function as neuronal target membrane (t)-SNAREs. However, little is known about their functional differences in synaptic transmission. STX1A null mutant mice develop normally and do not show abnormalities in fast synaptic transmission, but monoaminergic transmissi...

Apoptosis is a cell death modality that is initiated by the activation of caspases. Theoretically, fluorescence resonance energy transfer (FRET) analysis should be a convenient tool for visualizing the activation of caspase. Since the FRET probe cannot be transfected in primary neuronal cultures effectively, the FRET signal is not sufficiently stro...

The membrane protein HPC-1/syntaxin 1A is believed to play a key role in synaptic vesicle exocytosis, and it was recently suggested to be required for synaptic plasticity. Despite evidence for the function of HPC-1/syntaxin 1A in synaptic plasticity, the underlying cellular mechanism is unclear. We found that although fast synaptic transmission and...

The lateral accessory lobe (LAL) and the ventral protocerebrum (VPC) are a pair of symmetrical neural structures in the insect brain. The LAL-VPC is regarded as the major target of olfactory responding neurons as well as the control center for olfactory-evoked sequential zigzag turns. Previous studies of the silkworm moth Bombyx mori showed that th...

The concept of the mitochondrial permeability transition (mPT) has been used to explain cell death induced by calcium deregulation, which is in turn induced by a disruption in the mitochondrial loading capacity of cytosolic calcium (CLC). Whether mitochondria have specific morphologies representing the CLC and the mPT remains controversial. We exam...

In an earlier study, we showed that mitochondria hyperpolarized after short periods of oxygen-glucose deprivation (OGD), and this response appeared to be associated with subsequent apoptosis or survival. Here, we demonstrated that hyperpolarization following short periods of OGD (30 min; 30OGD group) increased the cytosolic Ca(2+) ([Ca(2+)](c)) buf...

Mitochondrial membrane potential (MMP) appears to play an important role in apoptotic cascade and has been proposed as an index for apoptosis or necrosis. We examined the neuroprotective effect of propofol on mode of death, focusing on MMP. Hippocampal cell culture was divided into three groups: control, oxygen-glucose deprivation for 30 min (30OGD...

The protein HPC-1/syntaxin 1A is abundantly expressed in neurons and localized in the neuronal plasma membrane. It forms a complex with SNAP-25 (25 kDa synaptosomal-associated protein) and VAMP-2 (vesicle-associated membrane protein)/synaptobrevin called SNARE (a soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex...

Mitochondrial membrane potential (MMP) regulates the production of high-energy phosphate and apoptotic cascade, both occurring after ischemic impact. The timed profile of MMP differing from grading ischemic impact has to be determined. Primary rat hippocampal cultures were exposed to oxygen-glucose deprivation (OGD) for 30, 60, and 90 min and then...

Ischemia limits the delivery of oxygen and glucose to cells and disturbs the maintenance of mitochondrial membrane potential (MMP). MMP regulates the production of high-energy phosphate and apoptotic cascading. Thus, MMP is an important parameter determining the fate of neurons. Differences in the time course of MMP according to the grading of the...

The membrane protein HPC-1/syntaxin 1A plays a key role in synaptic vesicle exocytosis in the presynaptic terminal. In particular, the H3 domain of HPC-1/syntaxin 1A participates in several protein-protein interactions that regulate neurotransmitter release. To investigate H3 domain function in neurotransmitter release, we used paired whole-cell pa...

In order to understand the neural mechanisms of pheromone-oriented walking in male silkworm moths, Bombyxmori, we have characterized olfactory responses and three-dimensional structure of two clusters (Group-I, Group-II) of descending interneurons in the brain by intracellular recording and staining with lucifer yellow. Neurons were imaged with las...

Male silkworm moths, Bombyx mori, move their heads side-to-side during zigzag walking toward a source of sex pheromone. High-speed video analysis revealed that changes in walking direction were synchronized with this head turning. Thus the direction of the walking is indicated by the direction of the head turning. Head turning was regulated by neck...

Male silkworm moths, Bombyx mori, exhibited sidewise movements of the head when they showed zigzagging walking in response to pheromonal stimulation. When the moth changed the direction of walking, the head angles also changed to those associated with the walking direction. The sidewise movements of the head were thought to be regulated by neck mot...

Thesis (Ph. D. in Science)--University of Tsukuba, (A), no. 2018, 1999.3.25