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Publications (25)
Withania ashwagandha, belonging to the family Solanaceae, is an important medicinal herb of India with restricted geographic distribution. It is a rich source of withaferin A (WA) and other bioactive withanolides. In the present study a rapid in vitro mass propagation protocol of W. ashwagandha was developed from nodal explants. Nodal explants were...
Withania somnifera (L) Dunal, commonly known as ashwagandha or Indian ginseng, is the source of large number of pharmacologically active withanolides. Withaferin-A (WS-3), a major withanolide of W. somnifera, has been proven to be an effective anti-cancer molecule. In this study, a liquid culture system for shoot proliferation, biomass accumulation...
A compilation of List of doctoral thesis (1968-2014)by Scientists Botanical Sciences Division while working at CSIR-RRL, jammu.
An in vitro regeneration system has been developed for Potentilla fulgens, which is an important Himalayan medicinal herb. Axillary shoot proliferation through shoot tip culture has been achieved on Murashige and Skoog (MS) medium containing 1mg l−1 6-benzylaminopurine (BAP) and 1 mg l−1 indole-3-acetic acid (IAA). Continuous production of plantlet...
Withania ashwagandha Kaul (Solanaceae) is an annual plant species of immense medicinal importance. It is a repository of a large number of pharmacologically active secondary metabolites known as withanolides. Evidence for the delimitation of the species from W. somnifera has been provided by our group using multidisciplinary approaches. Knowledge o...
The Indian germplasm of Withania somnifera (L.) Dunal shows remarkable genetic variability both in the cultivated and the wild populations. The utilization of this variability through conventional breeding requires a clear understanding of its reproductive biology and breeding system. It is an amphimictic species practicing open pollination. Howeve...
Genetic diversity of 23 accessions of Withania somnifera collected from different geographical regions of India was estimated by employing Random Amplification of Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) markers. Eighteen RAPD primers and six AFLP primer combinations revealed 37.82 and 43.94% polymorphism, respective...
Intraspecific variation in ITS regions of the rDNA among the five wild and five cultivated genotypes of Withania somnifera, were evaluated at nucleotide sequence level using restriction fragment length polymorphism (RFLP). The entire internal transcribed spacer (ITS1-5.8S-ITS2) region was first amplified by PCR and then cleaved with four different...
Realizing the inconsistencies that exist in the extent and nature of differentiation in the Withania somnifera genetic resources in India, the 21 cultivated and wild accessions, and the two hybrids (cultivated×wild accessions and
vice versa) were investigated for morphological, cytogenetical, chemical profiling, and crossability features. Their nuc...
We have developed a rapid in vitro propagation system, via axillary shoot formation from nodal explants of Swertia chirata Buch Ham. Culture medium supplemented with 2 mg/L. BAP is best for direct shoot regeneration initially formed adventitious buds from axils of the nodal explants after 30 days. The reduced BAP concentration 0.5 mg/L proliferate...
We have developed a rapid in vitro propagation system, via axillary shoot formation from nodal explants
of Swertia chirata Buch Ham. Culture medium supplemented with 2 mg/L. BAP is best for direct
shoot regeneration initially formed adventitious buds from axils of the nodal explants after 30 days. The
reduced BAP concentration 0.5 mg/L proliferate...
The present disclosure relates to methods and compositions for in vitro cultivation of species of Swertia, e.g. Swertia chirata (Ham.). The disclosure provides culture media comprising Murashige and Skoog (MS) basal culture medium, plant hormones preferably selected from the group consisting of benzyladenine (BAP), gibberellic acid (GA.sub.3), and...
An in vitro regeneration system has been developed for Potentilla fulgens, which is an important Himalayan medicinal herb.Axillary shoot proliferation through shoot tip culture has been achieved on Murashige and Skoog (MS) medium containing1mg l-1 6-benzylaminopurine (BAP) and 1 mg l-1 indole-3-acetic acid (IAA). Continuous production of plantlets...
Callus tissues derived from leaf segments of Onosma echioides var hispidum on three basal media viz. Murashige & Skoog (MS), Gamborg et al (B 5) and White's containing 3% sucrose produce napthaquinone pigments in presence of and NAA. However, β-β,dimethylacrylshikonin synthesis was triggered in dark in undifferentiated parenchyma cells on B5 agar m...
The present disclosure relates to methods and compositions for in vitro cultivation of species of Swertia, e.g. Swertia chirata (Ham.). The disclosure provides culture media comprising Murashige and Skoog (MS) basal culture medium, plant hormones preferably selected from the group consisting of benzyladenine (BAP), gibberellic acid (GA3), and auxin...
A micropropagation method has been developed for multiplication and conservation of Atropa acominata by induction of axillary shoot proliferation from shoot tips and nodal explants using Murashige and Skoog (MS) medium supplemented with BAP ( 1 mg I-1) and IBA (1 mg I-1). Revised tobacco (RT) medium with IAA (1 mg I-1) was found most suitable for s...
The present invention relates to the novel of culture media compositions, said compositions having modified Murashige and Skoog (MS) basal culture medium with varied concentrations of plant hormones and other additives, leading to extraordinarily faster in vitro propagation of Swertia Chirata, an endangered
medicinal plant species, wherein axillary...
