Suresh Babu Sringeri Vasudeva

Sri Sidhartha Academy of Higher Education · Central Research Facilities

A method for urinary peptide(s) and protein hydrolysis, involving autoclaving at 15 psi (121 • C) for 60 min, is described. Using three candidate proteins (bovine serum albumin, casein and gelatin) and urine specimens, the effect of autoclaving with respect to the optimum time required for hydrolysis under both acidic (6 N HCl) and alkaline (6 N KO...

Quantification of total and individual amino acids in biological fluids such as plasma, urine and cerebrospinal fluid has an important diagnostic implication in laboratory medicine. The present paper describes protocols for the assay of total amino acids by modified method based on dinitrophenyl and HPLC profile involving pre-column derivatization...

Vol. 45B, July 2006, 1747-52.

Oxidative stress abnormalities have been proposed to explain the pathogenesis of schizophrenia. The present study examined neopterin and oxidative stress abnormalities in schizophrenia patients before and after treatment. Serum neopterin, total anti-oxidants, nitrites and thiols in antipsychotic-naïve schizophrenia patients (n=45) were assessed at...

Background: Biological reference intervals (BRI) of most of the laboratory parameters are significantly influenced by lifestyle and need to be reviewed periodically. Objectives: To determine the reference intervals of common clinical-chemistry parameters such as fasting glucose, urea, creatinine, total cholesterol, triglycerides, HDL- C (Direct), L...

Pepsin, Obesity,

Background Serum Cys C has been acknowledged as a simple and reliable biomarker for assessing renal function in research studies. Evolving evidence show its efficacy in improving the diagnostic approach when included in routine clinical practice. Objectives The study intended to evaluate and compare serum Cys C sCys C levels in healthy individuals...

Osteoporosis is a bone disease resulting in reduction in bone mass and the bone become susceptible for fracture. The major factor involved in the development of osteoporosis includes, low calcium intake or decrease in Calcium absorption rate in aged population, bone strength in later life depends on development of bones earlier in life, adequate ca...

Original Research Paper Biochemistry INTRODUCTION: Albumin is the major protein present in the blood; any conditions causing lowering of albumin will lead to reduced total proteins in blood. This condition is called as Hypoproteinemia. Albumin is a 585 [2] amino acid polypeptide chain. It is only synthesized in liver. This is produced mainly by sal...

Calcium calmodulin dependent protein ser/thr phosphatase, also referred to as protein phosphatase 2B (PP2B), is rich in neural tissue, and plays an important role in the overall function of the nervous system. Routinely phosphatase assay employs, para-Nitrophenlylphosphate (p-NPP), as a substrate, is also extended to assay PP2B. However, in the pre...

Handigodu disease is a disorder of osteoarticular system, specific to particular region viz shimoga and chickmagalore districts of Karnataka state, India. The present study focuses to understand the metabolism of aggrecan, a key glycoprotein of cartilage matrix in Handigodu Disease (HD) using serum specimen. The Dimethylmethylene Blue Chloride (DMB...

Handigodu Disease (HD) is disorder of the osteoarticular system prevalent in few villages of two districts of the state Karnataka in southern India. 24 hrs urinary excretions of proline (Pro) and 4-hydroxyproline (Hyp) were analyzed by HPLC. Decreased peptide bound Hyp excretions (mumole/24 hrs) were found in patient group when compared with contro...

To establish/develop an assay method for measuring Ornithine Aminotransferase (EC.2.6.1.13) activity using rat brain mitochondria as a source of enzyme in presence and absence of Pyridoxal Phosphate (PLP). The modified method, with the improved sensitivity, is adopted for the assay of ornithine amino transferase activity in rat brain mitochondria....

Handigodu Disease (HD) is disorder of the osteoarticular system prevalent in few villages of two districts of the state Karnataka in southern India. 24 hrs urinary excretions of proline (Pro) and 4-hydroxyproline (Hyp) were analyzed by HPLC. Decreased peptide bound Hyp excretions ( μ mole/24 hrs) were found in patient group when compared with contr...

Assay of urinary imino acids, in particular peptide derived, is of immense utility in diagnosis of collagen-related disorders. The often-used methods for hydrolysis of urinary peptides need a long time and are cumbersome, hence the need for relatively simpler, but effective methods. The method described, based on alkaline hydrolysis by autoclaving...

Maple Syrup Urine Disease is an autosomal recessive disorder caused by a deficiency in the activity of the branched-chain α-ketoacid dehydrogenase complex. This rare disorder represents one of the causes of acute neonatal illness which results in devastating disturbances of neurological development. On investigation of 1750 infants with neurologica...