Smriti Shrivastava

Amity University | AU · Amity Institute of Biotechnology

The conversion of plant‐based lignocellulosic materials into alternative fuels entails various pretreatment strategies (chemical, physico‐chemical or enzymatic) to boost the release of sugar constituents from biomass. This investigation reports the influence of different pretreatment techniques such as acid, alkali and steam treatment followed by e...

The study aims to explore potential xylanase‐producing indigenous fungi isolated from soil and vegetable wastes containing plant degraded matter, reporting multilocus phylogenetic analysis and xylanase enzyme activity from selective strains. Four potential xylanolytic fungi were identified through distinct primary and secondary screening of 294 iso...

Lignocelluloses comprise of celluloses and hemicelluloses which can be effectively depolymerized to obtain fermentable sugars using diverse microbial enzymes, for subsequent conversion to various value-added products. Present study reports the bioprospecting of industrially significant microorganisms and their characterization to attain xylanases w...

In response to the scarcity of non-renewable energy sources, sustainable and renewable biofuels from biomass have gained utmost attention. Utilization of lignocellulosic biomass for production of varied energy forms (second generation fuels) like biogas, biodiesel, bioethanol, etc has increased in the past decade. Their properties of being naturall...

Microbial proteins are diverse and fascinating matter to study. Majority of time it happens that a protein cannot be studied at fine details as its properties are masked by others in vicinity. For the same, purification to homogeneity is required, and there are enormous number of techniques for protein purification currently followed in various lab...

Extracellular xylanase production by Thermomyces lanuginosus (NCIM-1374/DSM 28966) could be enhanced using statistical tools of Response surface methodology. Optimized process parameters (pH: 6.3; temperature: 52°C; fermentation time: 108 h; substrate concentration: 1.8% wheat bran) were obtained using ANNOVA. Saccharification process under optimiz...

Plant growth-promoting rhizobacteria (PGPR) are well known for their application in a wide range of agricultural crops and medicinally important plants toward growth enhancement, harvest yield, value addition to plants, etc. These various attributes are the result of varied mechanisms of which few are nitrogen fixation, quorum sensing, signal inter...

Piriformospora indica (Hymenomycetes, Basidiomycota) is a cultivable endophyte that colonizes roots and has been extensively studied. P. indica has multifunctional activities like plant growth promoter, biofertilizer, immune-modulator, bioherbicide, phytoremediator, etc. Growth promotional characteristics of P. indica have been studied in enormous...

Microbial association with plants has multidimensional attribute. Mycorrhizosphere microbes contribute effectively to disease resistance and nutrient cycling in plant and thus can be applied for crop development, optimization of plant health, nutrition, yield, etc. This chapter gives insight of root anatomy and microbe-associated complex root struc...

Extracellular cellulase free xylanase from Thermomyces lanuginosus sp. SS-8, isolated from self heating plant wreckage material was identified as β-1,4-endo-xylanase precursor, a monomer of 21.3 kDa with no carbohydrate residue. This xylanase retained 80 % activity at 60 °C for 96 h, was active at a wide pH range of 3–11 and uniquely hydrolyzed xyl...

Thermomyces lanuginosus SS-8 was isolated from soil samples that had been collected from near self-heating plant material and its extracellular cellulase-free xylanase purified approximately 160-fold using ion exchange chromatography and continuous elution electrophoresis. This xylanase was thermoactive (optimum temperature 60 °C) at pH 6.0 and had...

Fungal xylanases has important applications in food, baking, pulp and paper industries in addition to various other industries. Xylanases are produced extensively by both bacterial and fungal sources and has tremendous potential of being active at extremes of temperature and pH. In the present study an effort has been made to explore the codon bias...

Xylanases are unique hydrolytic enzymes with potential applications in industries. In the present study a complete comparative analysis of DNA and amino acid sequences from the xylanase precursor from Thermomyces lanuginosus has been carried out with respect to other fungal xylanases. A study was made on phylogenetic trees showing genetic and morph...

Optimization of different process parameters for lipase production by Rhizopus oryzae KG-10 and its validation using response surface methodology was carried out during the present study. Four various nutritional parameters were screened using Plackett–Burman experimental design were further optimized by Box–Behnken factorial design of response sur...

Deinococcus radiodurans R1 recovering from acute dose of gamma radiation shows a biphasic mechanism of DNA double-strand break repair. The possible involvement of microsequence homology-dependent, or non-homologous end joining type mechanisms during initial period followed by RecA-dependent homologous recombination pathways has been suggested for t...