Silvana Jukić Krmek

University of Zagreb · Department of Endodontics and Restorative Dentistry

The aim of this study was to compare the mechanical properties and ion release from a commercially available resin-modified glass ionomer cement to a formulation reinforced by the addition of short glass fibres at various percentages. Methods: Three experimental groups were prepared by adding a mass ratio of 10%, 15% and 20% of short glass fibres t...

To evaluate the 5-year clinical performance of a glass hybrid restorative system and a nano-hybrid resin composite in moderate to large two-surface class II cavities. This study was carried out by dental schools in Zagreb, Croatia; Izmir, Turkey; Belgrade, Serbia; and Milan, Italy. A total of 180 patients requiring two class-II two-surface restorat...

The aim of this study was to investigate the root canal morphology of lower second molars in the Croatian subpopulation of the Zagreb region using cone-beam computed tomography (CBCT). We analyzed 3212 CBCT scans from the two radiology centers in Zagreb. The number of roots and root canals, the occurrence of C-shaped canals and additional roots wer...

Objective: To compare short-term release of fluoride ions from ion-releasing dental restorative materials. Material and methods: Seven experimental groups were prepared using the following six different materials: alkasite (Cention Forte), resin-modified glass ionomer cement (Fuji II LC), bioactive composite (ACTIVA BioACTIVE-RESTORATIVE), fluoride...

Background: The purpose of this study was to examine the attitudes and practices of endodontists and paediatric dentists regarding RET. Material and methods: A survey was conducted among endodontists and paediatric dentists from 13 countries. A number of factors were evaluated, including frequency of RET application, followed guidelines, disinfecti...

Background An additional canal found in the mandibular first molar (M1M) is the middle mesial canal (MMC), which is often missed during root canal treatment. In this study, the prevalence of MMC in M1M on CBCT images was evaluated in 15 countries, along with the effect of some demographic factors on its prevalence. Method De-identified Cone Beam Co...

This study sought to evaluate the efficacy of SWEEPS in the removal of epoxy-resin-based and calcium-silicate-containing endodontic sealer combined with single-cone and carrier-based obturation techniques through a micro-CT analysis. Seventy-six single-rooted extracted human teeth with single root canal were instrumented with Reciproc instruments....

Over the past few decades, adhesive dentistry has advanced significantly. In light of minimal-invasive dentistry, this novel technique advocates a more conservative cavity design that relies on the efficiency of present enamel-dentine adhesives. The study aimed to address the scientific deficit in understanding the long-term bonding performance of...

Acidic drinks are known to exert negative effects on the surface properties of dental restorative materials. However, the effect of increasingly popular green smoothie drinks has not been addressed so far. The present study investigated the effect of cyclic immersions (5 min daily over 30 days) in a green smoothie drink on the surface properties of...

Acidic drinks are known to exert negative effects on the surface properties of dental restorative materials. However, the effect of increasingly popular green smoothie drinks has not been addressed so far. The present study investigated the effect of cyclic immersions (5 min daily over 30 days) in a green smoothie drink on the surface properties of...

The aim of this in vitro study was to assess the effect of two different adhesive application methods on shear dentin bond strength (ISO 29022) using three various adhesive systems. A mid-coronal section of 77 intact third human molars with fully developed apices was made to create flat bonding substrates. The materials used in the study were Excit...

Background: The aim of this study was to evaluate the effects of incorporation of hydroxyapatite (HA) derived from cuttlefish bone on the microhardness, surface roughness (SR), and fluoride release (FR) of conventional cure, and resin-modified glass-ionomer cement. Methods: There were four groups for each tested material; experimental glass-ionomer...

The objective of this study was to evaluate the efficacy of reciprocating instruments in the removal of bioceramic and epoxy resin-based sealers using micro-CT analysis. Root canals of 40 extracted human teeth were instrumented with a size R25 Reciproc instrument. Specimens were randomly divided into two groups (n = 20) according to the root canal...

Objectives: To determine the accuracy of visual damage evaluation of rotating and reciprocal endodontic instruments with the naked eye and optical devices. Material and methods: Four examiners were involved, divided into two age groups: I. 20-30 years, n = 2, II. 40-50 years, n = 2. They visually assessed the existence of damage to rotating or r...

The purpose of the study was to determine the wear rate of Equia Forte HT Fil with Equia Forte Coat or without coating and compare it with Fuji IX GP high-viscosity glass ionomer cement (GIC) in conditions with acid load or at neutral pH. The samples were stored for 7 days: (1) in artificial saliva, (2) in artificial saliva and cyclically exposed t...

Objective: The objective of the study, which consisted of a motivational lecture and hands-on training, was to evaluate the role of oral hygiene education for adolescents. Methods: The study population included sixty-two high school students between fourteen and fifteen years of age (thirty males and thirty-two females). The response rate was 76...

Introduction: Forces formed during root canal instrumentation could cause the crack formation in dentinal walls. Their propagation may result in vertical root fracture and eventually tooth loss. The aim of the study was to explore microcrack formation after root canal preparation with Self-adjusting File (SAF), Reciproc Blue (RB), and ProTaper Nex...

Objective: The objective of the study was to compare compressive strengths of two glass ionomerbased materials, with and without a light-cured, nano-filled coating, after cyclic loading and thermocycling. Materials and methods: To determine compressive strength of new restorative materials over a longer period of time, materials were analysed unde...

Objective: The objective of the study was to assess the quality of root canal fillings of cases referred to endodontic surgery using preoperative radiographs and correlate it with endodontic surgery treatment decision. The objective was also to analyse clinical symptoms and size of periapical lesions on radiographs and correlate them with treatment...

Aim The aim of this study was to assess dental students' perception of different learning environment in India, Nepal and Croatia. Methods The study was conducted during a period of academic year 2016/17. A total of 849 dental students participated in the study. There were 188 respondents from Croatia, 373 from India, and 288 from Nepal. Non-respo...

AIM: To examine the use of an Nd:YAG laser in removing gutta-percha fillings from root canals when used in conjunction with eucalyptol, dimethylformamide (DMF) or no solvent. METHODOLOGY: Root-canal fillings (sealer and gutta-percha) were removed with laser irradiation of 20 Hz/1.5 W from 30 roots randomly divided in three groups. In group 1, the...

The histological response of the dental pulp after laser irradiation was studied. After pulpotomy was performed in the premolar and molar teeth of dogs, the exposed pulp tissue at the root canal opening was lased using either a CO2 or Nd:YAG laser. The laser parameters were 2 W, 10 ms, 5 times per second for 1, 2 and 3 s for CO2 laser and 2 W, 20 p...

Objectives To determine the percentage of persistant apical lesions positive for bacterial nucleic acids, to detect microorganisms difficult to cultivate in persistant apical lesions by PCR and relate them to endodontic failure, clinical symptoms and diabetes mellitus. Materials and methods The samples of persistent apical lesions were collected d...

The purpose of this study was to determine the range of threshold responses of healthy dental pulps and test the reproducibility of the electric pulp test (EPT). Forty-nine dental students participated in the study. Sound teeth in both arches, except third molars, were included. The teeth were isolated using cotton rolls and dried with compressed a...

The purpose of the research was to compare oral health behavior and plaque indices between Croatian first-year and final-year dental students. Therefore, 46.9% of first-year and 47% of final-year dental students were surveyed using the Hiroshima University Dental Behavior Inventory (HU-DBI) questionnaire and had their plaque indices determined. Des...

Background: Bacterial resistance is considered a consequence of misuse or overuse of antibiotics. Dentistry significantly contributes to this increasing public health problem. The aim of this cross-sectional study was to examine the pattern of antibiotics prescribed by Croatian dentists in Zagreb area. Methods: Out of 220 Doctors of Dental Medic...

The aim: Epidemiologic studies in many countries show uneven distribution of oral diseases (primarily caries) within the population. This is why more studies are oriented towards specific regions or subpopulations instead of large scale national surveys. The major purpose of this cross sectional study was to obtain relevant data about the oral sta...

Objectives: to compare the level of anxiety reported by patients and assessed by dentists. Also, the expected and actual pain during the treatment perceived by the patient and dentist were assessed. Methods: sixty six endodontic patients filled in two questionnaires, prior to and after the treatment, so did their therapists. The first set of questi...

Objective: Aim of the study was to translate and test the psychometric properties of the OIDP (Oral Impacts on Daily Performances) index in order to make it available for usage in Croatian population. Method: OIDP questionnaire was translated from English to Croatian language by four postgraduate dental students. Backward translation was also...

Objective: to compare the level of anxiety reported by patients and assesed by dentists. Also, the expected and actual pain during the treatment perceived by the patient and dentist were assesed. Method: Sixty six endodontic patients filled in two questionnaires, prior to and after the treatment, so did their therapists. First set of questions fo...

Objective: to examine and compare differences in oral health behavior and plaque indices between dental students of first and final year at the University of Zagreb. Method: Cross-sectional study of 62 (68%) of the first and 47 (47%) of the final (6th) year dental students was conducted to compare differences in oral health behavior and plaque in...

Background Keratocystic odontogenic tumour (KCOT) is a benign, yet aggressive odontogenic tumour. Herein, proteome analysis of KCOT lesions in comparison with control patient-matched tissue unaffected by the disease and with inflammatory odontogenic cysts, namely radicular cysts is presented.Methods For the proteomics profiling, two complementary p...

Aim of this in vitro study was to compare accuracy of three electronic apex locators (EALs) (ES-02, ES-03 and ProPex) in root canals of extracted human teeth utilizing two meter readings and different conditions of root canals (RC). RC lengths of 46 single-rooted teeth were visually determined using a K-file # 15. Teeth and labial connector were th...

Unlabelled: The aim of this study was to compare the microleakage of class V cavities restored by silorane resin with those restored by the methacrylate resin system. Sixty standardized class V cavities were prepared on premolars. The teeth were restored with Filtek(TM) Silorane and Silorane(TM) System Adhesive, Filtek(TM) Silorane and Adper(TM) E...

The aim of this study was to evaluate the effect of Bluephase light emitting diode (LED) light on cell viability, colony-forming ability and proliferation in V79 cell culture and to determine how much the temperature of the nutrient medium rose. The investigation included a low (L), soft start (S) and high (H) illumination mode for 20, 40 and 80 se...

Objective: The aim of the present pilot- study was to evaluate the influence of oral intake of Ekolostrum (bovine colostrum) on concentration of salivary IgA(sIgA) in saliva. Materials and Methods: Twenty healthy participants between 23 and 54 years of age were randomly selected for the test and control group (n= 10). The test group took 60 capsule...

The aim of this study was to measure the concentration of fluorides in tap water in northern Croatian town Čazma and commercially available bottled drinks. Materials and Methods: Fluoride content was assessed by fluoride-selective electrode in tap water and commercial drinks from public supply. The pH was assessed by hydrogen ion-selective electrod...

To determine the prevalence of apical periodontitis and assess the quality of endodontic fillings in the population of the city of Zagreb, Croatia. A total of 1462 orthopantomograms from new patients at 6 different dental practices was analyzed during 2006 and 2007. The presence of periapical lesions was determined by using the periapical index sco...

Objectives: The aim of this study was to evaluate the role of motivation and education in oral hygiene of adolescents by mesuring plaque index. Methods: The study population consisted of 62 schoolchildren at the age of 14-15, first graders from First secondary high school in Zagreb. The plaque index was measured before and after a motivational and...

Objectives: To compare oral status of the fourth-year students of the School of Dental Medicine, and other fourth year students from the University of Zagreb. Materials and methods: Research included 78 dental students and 78 students of other colleges of the University of Zagreb. A standard dental check-up was performed following the WHO-prescribe...

The aim was to examine dental and periodontal status of adult Croatian 18th century population and compare the results with available literature data. 104 specimens from the crypt of Požega cathedral were grouped into three female and three male groups according to age. Caries was determined by visual inspection and dental probe. Ante and post mort...

The purpose of this study was to evaluate the antibacterial effect of root-end filling materials, MTA and amalgam, by agar diffusion test. Materials and methods: Blood agar plates were inoculated with bacteria Streptococcus mutans, Streptococcus mitis, Lactobacillus acidophilus, Staphylococcus aureus and Enterococcus faecalis each, and a polymicrob...

Cilj Ispitati antibakterijski učinak materijala za endodontsku opskrbu zuba. Metode Testom difuzije u agaru ispitivana su punila korijenskih kanala Ketac Endo, AH Plus, Diaket i gutaperka. Od materijala za podloge ispitivani su Cink Oksid/Eugenol cement, staklenoionomerni cementi Fuji II LC Improved i Ketac Cem, te Phosphatcement i Harvard cement....

To assess antimicrobial effects of the materials used in the endodontic treatment of the teeth. The following root-canal fillings were examined: Ketac Endo, AH Plus, Diaket, and gutta-percha, by means of the agar diffusion test. As for the base materials, Zink Oxide/Eugenol cement, glass-ionomere cements Fuji II LC Improved, Ketac Cem, and phosphat...

To compare the ability of the Variable Square Pulse technology (VSP-technology)-based erbium-doped yttrium aluminium garnet (Er:YAG) laser working in maximum speed (MAX) mode (1000 mJ, 300 micros, 20 Hz) and the high-speed handpiece to ablate enamel, to compare the ability of for the VSP-technology-based Er:YAG laser working in MAX mode and the low...

The aim of this study was to evaluate microleakage along resin restoration in cavities prepared with an erbium:yttrium-aluminium-garnet (Er:YAG) laser, with and without acid etching, and to compare it with that in diamond-drilled cavities. Thirty intact molars were divided into three equal groups. In the teeth in group I, class V cavities were prep...

Aim: To evaluate the dye leakage of three flowables with a new "three dimensional" method. Methods: Three groups of twelve premolars have received Class V cavities of uniform standardized dimensions. The cavities were restored with: Clearfil liner Bond 2 with adhesive SE Bond (group I), Definite flow with Etch & Prime 3.0 (group II) and, the Tetric...

The aim of this study was to examine the temperature changes in the pulp chamber during cavity preparation with the Er:YAG laser (2940 nm) with a very short pulse (VSP). Nine groups of 10 intact molars each were used. One root of each sample was amputated and a thermocouple was inserted into the chamber. Class V cavity preparation in enamel was per...

Undergraduate dental education changes students's perception of oral health, as well as oral hygiene habits and attitudes. Aim of this study was to compare oral hygiene and oral health habits and attitudes between 4th year students of dental medicine and 4th year students of other faculties of University of Zagreb in order to determine the possible...

Undergraduate dental education changes students’s perception of oral health, as well as oral hygiene habits and attitudes. Aim of this study was to compare oral hygiene and oral health habits and attitudes between 4th year students of dental medicine and 4th year students of other faculties of University of Zagreb in order to determine the possible...

The aim of this study was to evaluate the time and dose dependent effect of halogen light from dental curing unit on the cell viability, colony-forming ability and proliferation of the V79 cell culture. The investigation included the medium mode (M), exponential (E) and standard (S) illumination mode for 20, 40 and 80 seconds. The viability was det...

The purpose of this study was to evaluate vibrations induced by an erbium:yttrium-aluminum-garnet (Er:YAG) laser in the non-contact mode and compare the vibrations with different pulse durations and energy parameters. The experiment was conducted on an extracted tooth built up in silicone impression material. The vibrations were measured by piezoel...

The purpose of this study was to determine fragile histidine triad (FHIT) and p53 protein expression, and to analyze FHIT and p53 gene status in keratocystic odontogenic tumor (KOT), dentigerous cysts (DC) and radicular cysts (RC). The methods used were immunohistochemistry and molecular genetic methods including loss of heterozygosity (LOH) and ge...

The purpose of this study was to evaluate polymicrobial coronal leakage of mineral trioxide aggregate (MTA) and amalgam. There were 108 single-rooted teeth randomly divided into 3 groups of 32 teeth each and positive and negative control groups of 6 teeth and obturated with gutta percha and either Diaket (3M/ESPE, Seefeld, Germany), AH Plus (Dentsp...

The purpose of the study was to evaluate the general health condition i.e. overall health-status of the dental patients population by processing the data obtained from the FDI health-questionnaires which was recommended by the Croatian Dental Chamber. The data which was collected from a total of 2045 individual medical history questionnaires - pers...

To determine the prevalence and distribution of dilaceration in all tooth groups by using radiographs. The sample included 953 periapical intraoral radiographs and 488 panoramic radiographs from different caucasian patients. The ages of the patients ranged from 18-65 years. Dilaceration of the root was detected by measuring the degree of deviation...

The aim of this study was to determine the leakage of SuperEBA and intermediate restorative material (IRM) in root canal samples, with or without orthograde filling, by evaluating bovine serum albumin (BSA) microleakage using spectrophotometry. Thirty-five single-rooted teeth were divided into five groups, instrumented, and had apices resected. Roo...

The aim of this study was to compare the accuracy of measurements of endometers ES-02 and ES-03 in dry canals and canals filled with sodium-hypochlorite and ethylenediaminetetraacetic acid (EDTA). For the experiment 31 one-rooted human extracted teeth were used. Working length of the root canal was determined visually using a #15 reamer as a contro...

The aim of this in vitro study was to compare the sealing effectiveness of the Erbium:YAG laser to the sealing effectiveness of an ultrasonic device in the preparation of similar retrograde cavities using different retrograde filling materials. After root-canal instrumentation and filling, apices of 60 single-rooted teeth were resected. Retrograde...

Background and Objectives Variable square pulse (VSP) Er:YAG laser should be quicker than older Er:YAG lasers. The objectives were: (1) comparison of VSP laser and mechanical handpiece efficacy for apicectomy and (2) determination of optimal pulse width/energy/frequency combination.Study Design/Materials and Methods Sixty extracted, single-rooted m...

The aim of the study was to investigate the antibacterial effect of Nd:YAG laser in root canal samples using suspension of Enterococcus faecalis and Staphylococcus aureus. Twenty-two single-rooted teeth were instrumented, sterilized and inoculated with bacterial suspension concentration of 106 CFU/ml in brain-heart broth. The samples were irradiate...

The aim of this study was to examine mutagenic activity of four commercially available gutta-percha solvents by means of the Salmonella/ microsome assay. The examined solvents were: chloroform, orange oil, eucalyptus oil and halothane in amounts of 10 μl, 30 μl, 50 μl, 100 μl and 200 μl. Standard plate incorporation Ames test was preformed by using...

The aim of the investigation was to evaluate the amount of remaining gutta-percha and cement on dentine walls of the root canal after retreatment with three different gutta-percha solvents: eucalyptus oil, halothane and orange oil. Seventy one-rooted teeth were instrumented by “step-back” technique and filled with gutta-percha and Diaket cement usi...

The purpose of the study was to assess the patient’s attitude towards the use of a rubber dam and to determine whether any clinical factors influence it. After receiving endodontic treatment under a rubber dam, patients were asked to complete an anonymous questionnaire. They were divided into 2 groups: Group 1 - operators were final-year dental stu...

Three-rooted maxillary first premolars do not occur usually, but their possibility always has to be considered. The buccal orifices of the root canals are not clearly visible. In spite of a good knowledge of tooth anatomy, clinical experience, and X-rays, the third canal is often overlooked. The incidence of maxillary first premolars with three roo...

The aim of this study was to evaluate the expression of estrogen receptor (ER)-alpha in human pulp tissue. Tissue samples were collected from 51 teeth extracted for orthodontic reasons or from endodontically treated teeth. Immunohistochemical staining was performed with mouse antihuman ER-alpha 1D5 by using the alkaline phosphatase antialkaline pho...

A fluid transport model study was used to compare the sealing ability of five root canal sealers (AH26, AH Plus, Apexit, Diaket, and Ketac-Endo) on 60 single-rooted teeth after 1 yr of storage. The root canals were prepared with Gates Glidden drills by using a step-back technique before lateral condensation of gutta-percha with the tested sealers....

Inserting a self-threading pin can cause dentinal cracks, but it is not known whether dentin bonding agents can penetrate these cracks. Part I of this in vitro pilot study was conducted to document the presence of dentinal cracks after the placement of self-threading pins with 3 methods. Part II was conducted to observe changes in dentin when a den...

The aim of this study was to evaluate the antibacterial effect of Er:YAG laser in the root canal using bacteria of Enterococcus and Staphylococcus species. Eleven single-rooted teeth were instrumented, sterilized and ten of them inoculated with bacterial suspension of concentration 106 CFU/ml in brain heart broth. Nine samples were irradiated with...

Chloroform was the most frequently used solvent for removing guttapercha from the root canal. However, dispute with regard to its safety prompted the use of new solvents. The aim of this study was to compare the time required for dissolving gutta-percha by eucalyptol, halothane and orange oil. Seventy single rooted teeth were sterilised, treated by...

The aim of this study was to examine the mutagenic potential of canal sealers AH+ and AH26 by Salmonella/microsome assay. The materials were tested immediately after mixing, 1 hr and 1 month later, respectively. The dimethyl sulfoxide extracts of sealers in amounts of 3.0, 1.5, and 0.75 microliters/plate were used. The plated bacterial strains of S...

The purpose of the study was to evaluate cytotoxicity of AH Plus, compared to AH26 in vitro on Chinese hamsters V79 fibroblasts. The materials were prepared according to the manufacturer's instructions. After the initial setting period, the materials were crumbled and dissolved in dimetil-sulfoxide solution. The extracts obtained were incubated at...

The object of the study was to investigate where and in what way iconography and homage to St. Appolonia, patron saint of the dental profession, can be found in the Franciscan province of St. Cyril and Methodius. The study included 30 monasteries and counties and data were collected by means of a questionnaire, electronic communication and the exhi...