Sem Samuel Surja

Atma Jaya Catholic University of Indonesia | atmajaya · Parasitology

Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a potential and relatively simple rapid diagnostics method for COVID-19 detection. This study aims to evaluate and optimize the RT-LAMP performance on saliva specimens based on a commercially available kit.Modifications on an established protocol (Protocol A) wer...

Since January 2022, offline learning in the COVID-19 pandemic era has been going on for nearly a year. The government is attempting to revive the education sector, including universities. Nonetheless, this policy may result in the formation of a new SARS-CoV-2 infection cluster on campuses. One of the prevention measurements is conducting COVID-19...

Purpose: H. capsulatum is endemic in Indonesia, but the value of Histoplasma antigen detection has not been studied. Patients and methods: Histoplasma galactomannan (GM) ELISA was applied to sera of patients with unproven pulmonary tuberculosis (TB) and patients with a positive Aspergillus GM. Both Histoplasma and Aspergillus GM tests were perfo...

Atma Jaya Catholic University of Indonesia (AJCUI) COVID-19 Laboratory has become a reference testing site, which contributed to prevent the SARS-CoV-2 transmission in Indonesia. Through the LSSR and CARE, the Indonesian government has implemented moderate yet arguably successful policies to combat this pandemic. This study aims to assess and stren...

Invasive candidiasis (IC) has a high mortality rate of 70%, thus diagnosis should be established without delay. Given its fast result, serological test such as β-d-glucan (BDG) test is one alternative diagnosis modalities. However, it lacks specificity. Candida albicans germ tube antibody (CAGTA) test is an alternative serological test which has a...

Inflammatory bowel disease (IBD) consists of ulcerative colitis (UC) and Crohn’s disease (CD). The global incidence of IBD is increasing recently. Westernization is hypothesized to play role in this phenomenon. Inflammation is the basic etiology of IBD. Genetic and environmental factors are important determinants of IBD. Gastrointestinal microorgan...

Inflammatory bowel disease (IBD) consists of ulcerative colitis (UC) and Crohn’s disease (CD). The global incidence of IBD is increasing recently. Westernization is hypothesized to play role in this phenomenon. Inflammation is the basic etiology of IBD. Genetic and environmental factors are important determinants of IBD. Gastrointestinal microorgan...

Resistance of antifungal therapeutics has been reported, for instance, azole antifungal against Candida and Aspergillus. The discovery of new antifungal agent is thus warranted. Lagerstreomia speciosa is a notable Asian medicinal plant with profound antidiabetic activity. We previously demonstrated that the plant extract and one of the active subst...

Fungal infections have emerged as a significant and potentially lethal complication in critically ill patients with Covid-19. Infections caused by Aspergillus, Candida, and Mucormycetes, are a common cause of morbidity and mortality in critically ill patients, especially those with preexisting comorbidities. The underlying mechanisms that predispos...

Background: The demand for novel, useful, potential, and safe antifungal drugs and rapid fungal susceptibility test methods due to antifungal resistance and high prevalence of Candida albicans infection are continuing. Therefore, this study aimed to assess and compare the antifungal activity of lactoferrin, apolactoferrin, and oligosaccharides isol...

More sensitive diagnostic methods for detecting worms’ eggs are needed in areas with low soil-transmitted helminth (STH) prevalence. The Harada-Mori technique was first introduced by Mr. Harada and Mr. Mori. This technique is easy, simple, and does not require sophisticated equipment. The Harada-Mori technique is based on larvae tropism nature in t...

Saliva as a sample matrix has been an attractive alternative for the detection of SARS-CoV-2. However, due to potential variability in collection and processing steps, evaluating a proposed workflow amongst the local population is recommended. Here, we aim to validate the collection and treatment of human saliva as a direct specimen for RT-qPCR-bas...

Systemic fungal infection can disseminate to the skin and require prompt treatment, making early diagnosis very important. This study describes the use of a simple, quick touch biopsy method for the diagnosis of invasive mycoses in patients with AIDS with cutaneous manifestations. We identified fungal infections in 24 of the 29 investigated patient...

Saliva as a sample matrix has been an attractive alternative for the detection of SARS-CoV-2. However, due to potential variability in collection and processing steps, it is recommended to evaluate a proposed workflow amongst the local population. Here, we aim to validate collection and treatment of human saliva as a direct specimen for RT-qPCR bas...

Pendahuluan: Preservasi yang baik dan tepat dapat mempertahankan koleksi kultur dengan baik. Teknik preservasi subkultur berkala merupakan teknik lama yang kurang dapat menjamin sifat-sifat genetik jamur dalam jangka waktu lama. Air steril adalah salah satu metode alternatif dalam berbagai penelitian dalam menjamin sifat-sifat genetik jamur dalam w...

Background: A good collection of fungi culture can be maintained with good preservation methods. With appropriate methods, preservation can be performed in low-budget laboratories, such as with periodic subculture and preservation in either sterile water or in paraffin oil. The aim of this study was to assess the viability, morphology, contaminatio...

Objective Soil-transmitted helminth (STH) infections is prevalent in some areas in Indonesia. We predicted that there are few urban area hotspots, especially in the less developed urban area. This study aimed to determine the prevalence of STH infection in children and its association with environmental factor in Jakarta's less developed urban area...

Talaromycosis marneffei is a mycotic disease caused by Talaromyces marneffei which primarily infectimmunocompromised patient. This disease is endemic in Southeast Asia, and Indonesia consider as endemic area dueto a case of tourist who got the infection after visiting Indonesia. Diagnosis was made based on clinical suspicion withlaboratory confirma...

We performed morphology, molecular study and antifungal susceptibility test on 10 Talaromyces sp. isolates: eight clinical isolates (human immunodeficiency virus (HIV) and non-HIV-patient) and two isolates from rats. All strains produced red soluble pigment and microscopically showed Penicillium-like structure in room temperature and yeast-like str...

Some potentially human pathogenic fungi are known to be zoonoses. Rodent is one of potential reservoir of fungi. To date, little is known about the role of rodent in transmitting fungal disease in Indonesia. Therefore, purpose of this study was to find evidence of potential fungal zoonoses in rodent. We caught two house rats, one rat was from the h...

Background The prevalence of type 2 diabetes mellitus (DM) is increasing. Diabetic patients have a higher risk of getting dermatomycosis. Dermatomycoses, although a common health problem amongst DM, is often misdiagnosed and consequently undertreated. Studies on the association between dermatomycosis and type 2 diabetes are lacking, especially in...

Background Children with dysfunction in sensory integration(DSI) have difficulty effectively and efficiently interacting withtheir environment. It has been estimated that 5 to 10% ofchildrenwithout disabilities have DSI. Late assessment and interventionin children with this problem may significantly impact furtherdevelopment. To date, there is no a...

Background Children with dysfunction in sensory integration (DSI) have difficulty effectively and efficiently interacting with their environment. It has been estimated that 5 to 10% of children without disabilities have DSI. Late assessment and intervention in children with this problem may significantly impact further development. To date, there i...