Supporting info Environmental Effects of Nanoceria on Seed Production of Common bean (Phaseolus vulgaris)- A Proteomic anlaysis

Full text

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SUPPORTING INFORMATION
Environmental Effects of Nanoceria on Seed Production of Common bean (Phaseolus
vulgaris): A Proteomic Analysis
Sanghamitra Majumdar
a,c
,
Igor C. Almeida
e
, Emma A. Arigi
e
, Hyungwon Choi
f
, Nathan C.
VerBerkmoes
e
, Jesica Trujillo-Reyes
a
, Juan P. Flores-Margez
g
, Jason C. White
d
, Jose R. Peralta-
Videa
a,b,c
, Jorge L. Gardea-Torresdey
a,b,c*
a
Department of Chemistry, The University of Texas at El Paso, 500 West University
Ave., El Paso, TX 79968, USA
b
Environmental Science and Engineering PhD Program, The University of Texas at El
Paso, 500 West University Ave., El Paso, TX 79968, USA.
c
University of California Center for Environmental Implications of Nanotechnology (UC
CEIN), El Paso, Texas, USA.
d
Department of Analytical Chemistry, The Connecticut Agricultural Experiment Station,
123 Huntington Street, New Haven, Connecticut 06504, USA.
e
Border Biomedical Research Center, Department of Biological Sciences, The University
of Texas at El Paso, 500 West University Ave., El Paso, TX 79968, USA.
f
Saw Swee Hock School of Public Health, National University of Singapore and National
University Health System, Singapore.
g
Autonomous University of Ciudad Juarez, Departamento de Química y Biología,
Instituto de Ciencias Biomédicas, Anillo envolvente PRONAF y Estocolmo, Ciudad Juarez,
Chihuahua 32310, México
*Corresponding author: jgardea@utep.edu, Phone: 915-747-5359; Fax: 915-747-5748
Journal: Environmental Science and Technology
45 pages in length, including 5 figures and 8 tables.
Reference numbers stand for the reference list at the end.

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Supporting experimental procedures
Procedure S1. Soil source and characteristics
In this study, two types of soil varying in their organic matter content were used. The low
organic matter soil (LOMS) was collected from an agricultural field in Fabens, TX (N 31° 29’
02.1”, W 106° 08’ 27.2”, elevation 1102 m, mineral horizon, depth 55 cm) and was air dried
before use. The organic matter enriched soil (OMES) was prepared by mixing the LOMS with
Miracle-Gro potting mix at 2:1 (v/v) ratio. The air-dried soil samples were sub-sampled for
detailed characterization in triplicates as reported in Table S1. The elemental content in aqua-
regia digested soil samples were analyzed using inductively coupled plasma- optical emission
spectroscopy (ICP-OES).
1,2
The organic matter content of LOMS and OMES was 4% and 10%,
respectively, determined by the loss on ignition method. The dried soil samples were sub-
sampled for the analysis of mineral N by 1N KCl extraction and total kjeldahl nitrogen.
3
Owing to the low density of organic matter, similar volume of OMES weighed lower
than OMES. Thus, for nano-CeO
2
treatments, 3kg of OMES and 4kg of LOMS were used,
occupying the same volume in the pots.
Procedure S2. Determination of total soluble sugar and starch content
Total soluble sugar (TSS) from 100 mg samples were extracted three times in 10 ml of 80%
ethanol and boiled for 45 min, followed by centrifugation at 4,500 x g for 20 min (Eppendorf
5804R, Hamburg, DE).
4
The pooled supernatant was reduced to 5 ml by evaporation and diluted
to 25 mL with MPW. Starch in the seeds was extracted from the dried residue obtained after the
TSS extraction. The residues were suspended in 2 mL MPW and boiled in water bath for 15 min.
The suspensions were cooled to room temperature and 2 mL of 9.2 M HClO
4
was added to
hydrolyze starch to glucose, followed by 15 min stirring and dilution to 10 ml with MPW. The

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mixture was centrifuged at 3,000 x g for 20 in. The extraction was repeated two more times with
4.6 M HClO
4
, and the supernatants were pooled together and diluted to 40 mL with MPW. 100
µL of the extract was diluted to 1 mL with MPW. One mL 5% phenol and 5 mL 96% H
2
SO
4
were then added to the extract, which was vortexed and placed in water bath at 30° C) for 20
min. Analysis of TSS and starch was performed by phenol-sulfuric acid method according to
DuBois et. al. (1956) with minor modifications, and expressed as mg g
-1
dry weight of samples.
Starch content was calculated in terms of glucose equivalent (glucose x 0.9).
5
Blanks and a series of glucose standards, were used for quantification. The absorbance was read
at 490 nm and TSS content (mg in 100 mg sample) was determined using a standard calibration
curve. Starch content was calculated in terms of glucose equivalent (Glucose x 0.9). TSS and
starch content were expressed as mg g
-1
dry weight of samples. Glucose was used as standard.
5

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Supporting Information Figures
Figure S1. Schematic diagram for proteomic analysis of kidney bean seeds.

S5
Figure S2. Seeds per pod, seed fresh weight and percent moisture content in the seeds
harvested from plants exposed to 0-500 mg/kg nano-CeO
2
in low organic matter soil
(LOMS) and organic matter enriched soil (OMES). Values are expressed as mean ± SE
(n=4). Bars with different letters within same soil type, represent significant difference with
respect to control at p ≤ 0.05.

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Figure S3. Micronutrients (µg/kg dry wt) in carpels and seeds harvested from plants
exposed to 0-500 mg/kg nano-CeO
2
in low organic matter soil (LOMS) and organic matter
enriched soil. (OMES). Values are expressed as mean ± SE (n=4). Bars with *, ** and ***
within same soil type, represent significant difference at p ≤ 0.1, 0.05 and 0.01, respectively.

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Figure S4. (a) Crude protein, (b) total soluble sugar, and (c) starch content in seeds
harvested from plants exposed to 0-500 mg/kg nano-CeO
2
treatments in low organic matter
soil (LOMS) and organic matter enriched soil (OMES). Values are expressed as mean ± SE
(n=3). Bars with different letters within same soil type, represent significant difference at p
≤ 0.05.

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Figure S5. Principal component analysis (PCA) using spectral count values from 44
differentially expressed proteins (FDR ≤ 0.2), as compared to control, from seeds harvested
from plants exposed to 0-500 mg/kg nano-CeO
2
.

S9
Supporting Information Tables
Table S1. Characteristics of low organic matter soil (LOMS) and organic matter enriched
soil (OMES)
Soil Characteristics LOMS OMES
pH 7.90 ± 0.09 7.30 ± 0.05
Organic matter (%)* 4.22 ± 0.01 10.1 ± 0.08
Electrical conductivity(µS/cm)* 208 ± 4.79 485 ± 35.7
Cation exchange capacity (meq/100g)* 23.3 ± 1.78 33.1 ± 1.28
Total dissolved solid (mg/l)* 92.5 ± 2.5 240 ± 19.2
Total elemental content (mg kg-1 soil)
Aluminum 21,200 ± 95.2 20,900 ± 783
Calcium 25,960 ± 81.9 27,100 ± 941
Cerium 38.4 ± 0.850 33.8 ± 1.04
Copper* 13.9 ± 0.057 24.4 ± 1.42
Iron 14,600 ± 59.2 14,300 ± 453
Potassium* 5,350 ± 113 5,980 ± 262
Magnesium 6,030 ± 29.6 6,151 ± 249
Manganese 351 ± 2.56 376 ± 22.3
Nickel 8.00 ± 0.119 8.40 ± 0.931
Nitrogen (inorganic)* 48.0 ± 2.24 85.4 ± 5.54
Nitrogen (total kjeldahl)* 654 ± 21.6 917 ± 37.2
Phosphorus 910 ± 5.70 846 ± 30.2
Sodium 1,420 ± 67.9 1,297 ± 84.8
Sulfur* 653 ± 11.0 779 ± 41.5
Zinc* 43.2 ± 1.09 55.3 ± 3.07
The values are expressed as mean ± SE (n=3). * denotes significant difference among LOMS and OMES at p ≤ 0.05.

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Table S2. Yield-related parameters for plants exposed to 0-500 mg/kg nano-CeO
2
in low
organic matter soil (LOMS) and organic matter enriched soil (OMES) for 50 d.
Soil type
LOMS OMES
nano-CeO
2
treatment (mg/kg) 0 62.5 125 250 500 0 62.5 125 250 500
Pods per plant 2.90 ±
0.32
a
3.40 ±
0.25
a
3.57 ±
0.12
a
3.56 ±
0.38
a
3.80 ±
0.20
a
6.29 ±
0.40
ab
5.57 ±
0.30
a
6.89 ±
0.06
b
6.34 ±
0.24
ab
6.27 ±
0.24
ab
Pod
length
(cm)
Maximum
length
10.1 11.3 12.4 11 11.7 13.2 12.7 14.8 14.2 14.2
≥7 8.80 ±
0.51
a
9.29 ±
0.30
a
10.1 ±
0.43
a
8.90 ±
0.28
a
8.98 ±
0.31
a
10.23 ±
0.26
a
10.15
± 0.26
a
10.7 ±
0.24
a
10.61 ±
0.27
a
10.4 ±
0.25
a
<7 4.53 ±
1.61
a
4.28 ±
0.36
a
4.44 ±
0.48
a
4.39 ±
0.26
a
4.58 ±
0.48
a
5.46 ±
0.21
a
5.15 ±
0.28
a
5.52 ±
0.29
a
5.76 ±
0.22
a
5.87 ±
0.18
a
Values are expressed as mean ± SE (n=4). The values with different letters within each soil type represent significant difference at p ≤ 0.05.
Table S3. Macronutrient composition (mg kg
-1
dry wt) in kidney bean carpels and seeds
collected from plants exposed to 0-500 mg/kg nano-CeO
2
in low organic matter soil
(LOMS) and organic matter enriched soil (OMES).
Nutrients
nano-CeO2
Treatments
Carpels Seeds
Ca K Mg P S Ca K Mg P S N Na:K
LOMS 0 8640 ±
632ab
24645 ±
1544a
3757 ±
130a
3613 ±
309a
1646 ±
140a
1230 ±
149a
15218 ±
98a
1880 ±
74a
6757 ±
475a
2783 ±
143a
36528 ±
648a
0.006 ±
0.001a
62.5 8264 ±
157a
25359 ±
678a
3727 ±
120a
4168 ±
148a
1771 ±
93a
1451 ±
286a
15019 ±
1065a
1815 ±
188a
5930 ±
937a
2399 ±
270a
33874 ±
1902a
0.005 ±
0.001a
125 6890 ±
390a
23574 ±
1355a
3103 ±
64a
3299 ±
254a
1635 ±
172a
1067 ±
58a
13746 ±
706a
1752 ±
122a
5313 ±
151a
2601 ±
50a
41760 ±
647b
0.006 ±
0.000a
250 11152 ±
639b
27185 ±
1031a
3806 ±
184a
3992 ±
276a
1983 ±
92a
1487 ±
230a
16641 ±
504a
1944 ±
65a
5432 ±
184a
2606 ±
88a
37615 ±
1801a
0.004 ±
0.000a
500 8571 ±
979ab
23711 ±
2039a
3446 ±
312a
3831 ±
507a
1748 ±
173a
1586 ±
103a
16880 ±
406a
1963 ±
33a
7380 ±
165a
2674 ±
29a
35651 ±
1123a
0.005 ±
0.001a
OMES 0 6885 ±
462a
21385 ±
1915a
2996 ±
130a
3230 ±
302a
1415 ±
159a
1513 ±
205a
16449 ±
836a
1905 ±
66a
8293 ±
512a
3028 ±
100a
35310 ±
1307a
0.005 ±
0.000a
62.5 7204 ±
767a
24017 ±
0a
3162 ±
80a
4819 ±
210b
2353 ±
223b
1450 ±
159a
15473 ±
1389a
1782 ±
135a
7966 ±
780a
2755 ±
228a
34870 ±
1820a
0.005 ±
0.001a
125 5851 ±
268a
24070 ±
962a
2785 ±
56a
4294 ±
250ab
2190 ±
160b
1818 ±
266a
17641 ±
571a
1930 ±
41a
7374 ±
231a
2587 ±
57a
30141 ±
1153a
0.003 ±
0.000a
250 6670 ±
1231a
23217 ±
545a
3067 ±
338a
4068 ±
534ab
2338 ±
161b
1910 ±
312a
17801 ±
641a
1973 ±
103a
9646 ±
860a
3095 ±
301a
31156 ±
1957a
0.005 ±
0.001a
500 4791 ±
437a
20646 ±
363a
2696 ±
150a
3082 ±
274a
1640 ±
109ab
2104 ±
78a
17634 ±
165a
2042 ±
26a
9263 ±
394a
2945 ±
77a
31689 ±
849a
0.003 ±
0.000a
Values are expressed as mean ± SE (n=4). The values with different letters within each soil type represent significant difference at p ≤ 0.05.

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Table S4. Two way ANOVA for nutrient quality of kidney bean carpels and seeds from
plants exposed to 0-500 mg/kg nano-CeO
2
in low organic matter soil (LOMS) and organic
matter enriched soil (OMES). SS: Type III Sum of squares, MS: Mean square, Conc: nano-
CeO
2
concentration, SOM: Soil organic matter.
Carpel Seed
Nutrient SS df MS F Sig. SS df MS F Sig.
Conc Ce 114924 4 28731.04 6.54 0.002 770 4 192.45 4.25 0.012
Ca 32492504 4 8123125.89 4.49 0.006 936934 4 234233.38 1.61 0.211
Mg 1712019 4 428004.78 3.25 0.025 260344 4 65085.93 2.08 0.122
P 6301676 4 1575419.06 3.67 0.015 12464908 4 3116227.12 3.04 0.041
K 101610246 4 25402561.51 1.28 0.300 28336172 4 7084042.89 2.80 0.054
S 2153100 4 538275.05 5.75 0.001 743832 4 185957.96 1.74 0.181
B 51014254 4 12753563.40 1.39 0.260 3471244 4 867810.89 2.05 0.117
Cu 3670599 4 917649.78 14.44 0.000 453477 4 113369.14 0.63 0.642
Fe 731889166 4 182972291.41 2.48 0.065 563168953 4 140792238.36 1.66 0.190
Mn 68194902 4 17048725.58 1.77 0.162 3332273 4 833068.20 0.79 0.541
Mo 49519699 4 12379924.83 1.89 0.137 2884941 29 4 72123532.16 20.33 0.000
Ni 1476044 4 369011.01 3.83 0.012 866076 4 216519.03 1.34 0.285
Zn 68362996 4 17090748.90 3.05 0.032 90943348 4 22735837.02 4.10 0.011
Na 661 4 165.31 0.61 0.662 795 4 198.84 0.63 0.645
N
25135167 4 6283791.84 1.05 0.405
Protein
10 4 2.45 1.05 0.406
TSS
0 4 0.12 0.52 0.725
Starch
1 4 0.21 1.92 0.146
SOM Ce 84724 1 84724.01 19.28 0.000 366 1 365.61 8.07 0.010
Ca 58704368 1 58704368.41 32.44 0.000 1652299 1 1652299.01 11.35 0.003
Mg 3922364 1 3922364.00 29.76 0.000 26190 1 26190.08 0.84 0.372
P 139850 1 139850.27 0.33 0.572 52244984 1 52244984.33 50.94 0.000
K 11006454 1 11006454.14 0.55 0.462 16123043 1 16123042.58 6.38 0.020
S 531651 1 531651.07 5.68 0.024 793901 1 793900.89 7.42 0.013
B 528332439 1 528332439.22 57.72 0.000 11 1 11.37 0.00 0.996
Cu 11908622 1 11908621.82 187.44 0.000 3005322 1 3005322.26 16.83 0.000
Fe 2202841477 1 2202841476.76 29.86 0.000 1766627685 1 1766627684.92 20.87 0.000
Mn 556559338 1 556559337.53 57.68 0.000 20116097 1 20116097.42 19.13 0.000
Mo 91635052 1 91635052.04 14.02 0.001 929748082 1 929748081.95 262.09 0.000
Ni 10478202 1 10478202.09 108.88 0.000 4540406 1 4540406.17 28.00 0.000
Zn 278026299 1 278026298.71 49.62 0.000 66010669 1 66010668.84 11.90 0.002
Na 483 1 482.76 1.77 0.194 648 1 648.19 2.06 0.164
N
148691234 1 148691234.03 24.95 0.000
Protein 58 1 58.02 24.93 0.000
TSS
0 1 0.02 0.09 0.772
Starch
13 1 12.66 114.71 0.000
Conc x
SOM Ce 28059 4 7014.79 1.60 0.214 1636 4 408.90 9.03 0.000
Ca 58704368 1 58704368.41 32.44 0.000 828369 4 207092.28 1.42 0.263
Mg 3922364 1 3922364.00 29.76 0.000 64956 4 16238.99 0.52 0.723
P 139850 1 139850.27 0.33 0.572 9864265 4 2466066.32 2.40 0.084
K 11006454 1 11006454.14 0.55 0.462 9130091 4 2282522.74 0.90 0.481
S 531651 1 531651.07 5.68 0.024 354875 4 88718.85 0.83 0.522
B 37078849 4 9269712.35 1.01 0.416 14128631 4 3532157.84 8.36 0.000
Cu 1221846 4 305461.44 4.81 0.004 815216 4 203804.07 1.14 0.360
Fe 512656919 4 128164229.85 1.74 0.168 1501941095 4 375485273.82 4.44 0.008
Mn 25656851 4 6414212.73 0.66 0.621 12307005 4 3076751.33 2.93 0.041
Mo 37340891 4 9335222.67 1.43 0.249 187587794 4 4689694 8.53 13.22 0.000
Ni 1636087 4 409021.72 4.25 0.008 1541700 4 385424.92 2.38 0.079
Zn 12935148 4 3233787.05 0.58 0.681 69525972 4 17381493.11 3.13 0.032
Na 330 4 82.42 0.30 0.874 2081 4 520.33 1.65 0.193

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N 143671659 4 35917914.85 6.03 0.002
Protein 56 4 14.04 6.03 0.002
TSS 3 4 0.66 2.88 0.049
Starch 1 4 0.26 2.39 0.085
Table S5. FTIR vibrational shifts corresponding to band regions in the seeds obtained from
plants exposed to 0-500 mg/kg nano-CeO
2
. The band regions have been referred from Dokken et
al.
34
and Taoutaou et al.
32
Low organic matter soil
nano-CeO
2
(mg/kg)
Vibrational shifts
Lipids Lipids
Lignin/ Amide II Amide I Carbohydrate Lignin
Control 3284
2925
1739 1635 1539 1242
1146
1074
853
62.5 3277
2926
1736 1640 1542 1242
1146
1074
851
125 3275
2925
1735 1640 1542 1242
1145
1074
853
250 3276
2926
1735 1635 1542 1242
1147
1074
852
500 3285
2926
1735 1640 1542 1241
1145
1074
852
Organic matter enriched soil
nano-CeO
2
(mg/kg)
Vibrational shifts
Lipids Lipids
Lignin/ Amide II Amide I Carbohydrate Lignin
Control 3285
2925
1735 1640 1542 1239
1147
1074
853
62.5 3277
2926
1735 1640 1542 1238
1147
1074
853
125 3276
2925
1735 1640 1542 1238
1146
1074
852
250 3275
2925
1735 1640 1542 1238
1146
1074
851
500 3276
2924
1734 1640 1542 1242
1147
1075
853

S13
Table S6.
List of kidney bean seed proteins identified and quantified by MS/MS analysis.
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
T2DMA9
14-3-3 family protein
OS=Phaseolus vulgaris PE=2
SV=1
-1.30 -0.13 1.00 0.93 -0.10 -0. 31 0.89 1.00 0.46 1.49 0.84 0.99 -0.76 -1.94 1.00 0.61
T2DP01 14-3-3 protein OS=Phaseolus
vulgaris PE=2 SV=1 -0.29 -0.03 0.98 1.00 0.10 0.55 1.00 0.95 -0.07 -0.31 0.93 1.00 -0.15 -0.77 0.91 0.99
T2DNK7 14-3-3 protein OS=Phaseolus
vulgaris PE=2 SV=1 -0.67 -0.06 0.99 1.00 0.43 1.03 1.00 0.97 0.30 0.64 1.00 0.98 -0.10 -0.22 0.89 0.98
C6SVV7 40S ribosomal protein S12
OS=Glycine max PE=2 SV=1 -0.28 -0.03 0.98 1.00 - 0.78 -1.04 0.99 0.96 0.72 1.29 0.9 6 0.99 -0.78 -1.04 0.95 0.95
C6SWG4 40S ribosomal protein S21
OS=Glycine max PE=3 SV=1 0.74 0.07 0 .99 1.00 -1.20 -1.71 1.00 0.66 -0.47 -0.74 0.96 1.00 -0.63 -1.10 0.96 0.93
C6SWV6 40S ribosomal protein S27
OS=Glycine max PE=3 SV=1 -2.04 -0.21 1.00 0.82 - 0.52 -0.87 0.97 1.00 -1.20 -1.39 1.00 0.86 -0.32 -0.58 0.89 1.00
C6TLT3 40S ribosomal protein S3a
OS=Glycine max PE=2 SV=1 0.55 0.05 0 .98 1.00 0.31 0.55 1.00 0.95 0.57 0.97 1.00 0.98 0.31 0.55 1.00 0.91
T2DNT1
40S ribosomal protein S5-like
protein OS=Phaseolus vulgaris
PE=2 SV=1
-0.06 -0.01 0.98 1.00 -0.40 -1. 32 1.00 0.84 -0.48 -1.35 1.00 0.88 -0.40 -1.32 0.99 0.87
B2BF98 40S ribosomal protein S6
OS=Glycine max PE=3 SV=1 -1.99 -0.21 1.00 0.82 - 0.35 -0.43 0.90 1.00 -0.44 -0.47 0.93 1.00 -0.35 -0.43 0.88 1.00
C6T514
40S ribosomal protein S8
(Fragment) OS=Glycine max PE=2
SV=1
1.03 0.10 0.99 1.00 -0.32 -0.80 0.95 1.00 0.47 1.28 0.96 0.99 -0.08 -0.22 0.89 0.98
O22518 40 S ribosomal protein SA
OS=Glycine max PE=1 SV=1 0.08 0.01 0 .98 1.00 -0.21 -0.54 0.91 1.00 0.35 0.96 1.00 0.98 -1.80 -2.69 1.00 0.31
C6TEJ6 40S ribosomal protein SA
OS=Glycine max PE=2 SV=1 0.01 0.00 0 .98 1.00 -0.01 -0.01 0.92 1.00 -1.04 -1.19 1.00 0.94 -0.95 -1.31 0.99 0.87
Q39801 51 kDa seed maturation protein
OS=Glycine max PE=2 SV=1 0.74 0.07 0 .99 1.00 0.00 -0.01 0.93 1.00 0.04 0.10 1.00 0.97 0.06 0.17 1.00 0.92
T2DLV0
5-
methyltetrahydropteroyltriglutamate
homocysteine methyltransferase
like isoform 1 OS=Phaseolus
vulgaris PE=2 SV=1
2.07 0.21 0.93 1.00 -0.18 -1.48 1.00 0.76 -0.16 -1 .19 1.00 0.94 -0.35 -2.71 1.00 0.31
E7E8T8
60S acidic ribosomal protein P0
OS=Phaseolus vulgaris PE=2
SV=1
-0.92 -0.09 1.00 1.00 -0.71 -2. 35 1.00 0.33 -0.58 -1.74 1.00 0.70 -0.91 -2.78 1.00 0.28
T2DPD1
60S acidic ribosomal protein P1-3-
like protein OS=Phaseolus vulgaris
PE=4 SV=1
-0.06 -0.01 0.98 1.00 -1.32 -1. 87 1.00 0.57 -1.40 -1.65 1.00 0.75 -1.32 -1.87 1.00 0.64
T2DN44
60S ribosomal protein L13-2-like
protein OS=Phaseolus vulgaris
PE=2 SV=1
0.85 0.09 0.99 1.00 -1.32 -1.86 1.00 0.58 -0.59 -0 .93 0.98 1.00 -1.32 -1.86 1.00 0.65
T2DNF1
60S ribosomal protein L13a
OS=Phaseolus vulgaris PE=2
SV=1
-0.06 -0.01 0.98 1.00 -0.26 -0. 52 0.91 1.00 -0.81 -1.15 1.00 0.95 -0.63 -1.10 0.96 0.93

S14
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
T2DMQ9
60S ribosomal protein L18-3-like
protein OS=Phaseolus vulgaris
PE=2 SV=1
0.60 0.07 0.99 1.00 -0.14 -0.37 0.89 1.00 -0.46 -0 .98 0.98 1.00 -0.49 -1.17 0.97 0.91
T2DLT1
60S ribosomal protein L3-like
protein OS=Phaseolus vulgaris
PE=2 SV=1
0.82 0.08 0.99 1.00 -0.49 -0.95 0.98 1.00 -1.52 -1 .74 1.00 0.70 -0.49 -0.95 0.94 0.96
T2DLN4
60S ribosomal protein l9
OS=Phaseolus vulgaris PE=2
SV=1
0.57 0.05 0.98 1.00 -0.30 -0.84 0.96 1.00 -0.31 -0 .79 0.96 1.00 -0.95 -2.23 1.00 0.49
I1KWM7
6-phosphogluconate
dehydrogenase, decarboxylating
OS=Glycine max PE=3 SV=1
4.30 0.43 0.72 1.00 0.32 0.70 1.00 0.96 -0.01 -0.02 0.99 0.98 -0.11 -0.22 0.89 0.98
N6V3T9
ABC transporter integral
membrane protein OS=Rhizobium
freirei PRF 81 GN=RHSP_79899
PE=4 SV=1
1.36 0.13 0.99 1.00 -0.57 -0.72 0.94 1.00 -0.65 -0 .71 0.95 1.00 -0.57 -0.72 0.91 1.00
T2DLM1
Acetohydroxyacid
isomeroreductase OS=Phaseolus
vulgaris PE=2 SV=1
1.87 0.18 0.96 1.00 0.01 0.02 0.93 1.00 -0.07 -0.07 0.98 0.99 0.01 0.02 0.98 0.94
T2DP56 Acid phosphatase OS=Phaseolus
vulgaris PE=2 SV=1 0.49 0.05 0.98 1.00 -0.60 -0.92 0.97 1.00 -1.03 -1 .15 1.00 0.95 -0.93 -1.27 0.99 0.88
O65016 Acti n 4 OS=Glycine max GN=SAc4
PE=3 SV=1 0.23 0.02 0.98 1.00 -0.40 -1.26 1.00 0.87 -0.35 -0 .99 0.98 0.99 -0.60 -1.75 1.00 0.69
C6SVF1
Actin depolymerizing factor 1
OS=Glycine max GN=ADF1 PE=2
SV=1
0.18 0.02 0.98 1.00 0.70 2.43 0.18 0.99 0.35 1.02 1.00 0.98 0.29 0.94 1.00 0.93
F8SMB9 Adenosylhomocysteinase
OS=Glycine max PE=2 SV=1 -0.66 -0.07 0.99 1.00 0.0 2 0.18 1.00 0.98 -0.15 -0.94 0.98 1.00 -0.26 -1.80 1.00 0.67
T2DN08
ADP-ribosylation factor
OS=Phaseolus vulgaris PE=2
SV=1
1.61 0.17 0.96 1.00 -0.42 -1.09 1.00 0.94 -0.94 -1 .79 1.00 0.68 -1.14 -2.33 1.00 0.44
F8QXQ1 Albumin-1A OS=Phaseolus
vulgaris PE=2 SV=1 0.47 0.04 0.98 1.00 -0.95 -1.29 1.00 0.86 -1.05 -1 .18 1.00 0.94 -0.95 -1.29 0.99 0.88
F8QXP8 Albumin-2 OS=Phaseolus vulgaris
PE=2 SV=1 -0.15 -0.01 0.98 1.00 -0.03 -0. 31 0.89 1.00 -0.04 -0.44 0.93 1.00 -0.03 -0.37 0.88 1.00
T2DLR9
Alcohol dehydrogenase 1
OS=Phaseolus vulgaris PE=2
SV=1
-2.04 -0.23 1.00 0.83 -0.04 -0. 55 0.91 1.00 -0.07 -0.85 0.97 1.00 -0.14 -1.92 1.00 0.62
T2DMX0
Alcohol dehydrogenase class III
OS=Phaseolus vulgaris PE=2
SV=1
-0.28 -0.03 0.98 1.00 0.19 1.17 1.00 0.98 0.03 0.15 1.00 0.97 -0.20 -1.13 0.97 0.92
I1KAB0 Aldehyde dehydrogenase
OS=Glycine max PE=3 SV=1 0.02 0.00 0 .98 1.00 -0.52 -1.41 1.00 0.80 -0.85 -1.77 1.00 0.69 -1.70 -2.98 1.00 0.23
H6WQB3 Allantoinase 1 OS=Phaseolus
vulgaris PE=2 SV=1 -0.13 -0.01 0.98 1.00 -0.53 -1. 98 1.00 0.52 -0.93 -2.63 1.00 0.29 -1.15 -3.46 0.98 0.12
A0T2V2
Alpha amylase inhibitor-1
OS=Phaseolus vulgaris PE=4
SV=1
2.78 0.31 0.85 1.00 0.07 1.16 1.00 0.98 -0.11 -1.60 1.00 0.77 -0.05 -0.80 0.92 0.99
Q6J2U4
Alpha amylase inhibitor-1
OS=Phaseolus vulgaris PE=4
SV=1
-1.25 -0.12 1.00 0.94 0.04 0.47 1.00 0.95 -0.10 -0.89 0.97 1.00 -0.11 -1.16 0.97 0.91
T2DNR4
Alpha-1,4-glucan-protein synthase
[UDP-forming]-like protein
OS=Phaseolus vulgaris PE=2
SV=1
-1.69 -0.17 1.00 0.86 -0.12 -1. 07 0.99 0.95 0.07 0.59 1.00 0.97 -0.27 -2.21 1.00 0.49

S15
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Q9S9E1
Alpha-amylase inhibitor beta
subunit, PHA-I beta subunit
OS=Phaseolus vulgaris PE=4
SV=1
-0.86 -0.08 1.00 1.00 -0.01 -0. 07 0.91 1.00 -0.09 -1.19 1.00 0.93 -0.15 -2.05 1.00 0.56
Q41115 Al pha-phaseolin OS=Phaseolus
vulgaris PE=4 SV=1 1.49 0.15 0.98 1.00 -0.14 -4.18 0.99 0.00 -0.06 -1 .59 1.00 0.77 -0.13 -3.78 0.97 0.06
C6THM9 Annexin OS=Glycine max PE=2
SV=1 -2.78 -0.32 1.00 0.85 -0.52 -1. 32 1.00 0.84 -0.76 -1.54 1.00 0.80 -0.61 -1.53 1.00 0.79
C6T7M2 Annexin OS=Glycine max PE=2
SV=1 -0.46 -0.04 0.98 1.00 1.52 2.20 0.33 0.99 0.74 0.90 1.00 0.98 1.61 2.33 1.00 0.95
I1M0U7 Annexin OS=Glycine max PE=3
SV=1 -2.95 -0.31 1.00 0.89 0.11 0.33 1.00 0.96 -0.37 -0.89 0.97 1.00 -0.57 -1.47 1.00 0.81
P84869
Antifungal lectin PVAP (Fragment)
OS=Phaseolus vulgaris PE=1
SV=1
-2.37 -0.24 1.00 0.83 -0.50 -4. 33 0.99 0.00 -0.70 -4.94 0.99 0.00 -0.59 -4.98 0.96 0.00
T2DMN2
Aquaporin PIP-type 7a-like protein
OS=Phaseolus vulgaris PE=2
SV=1
0.29 0.03 0.98 1.00 -0.01 -0.01 0.92 1.00 -0.86 -0 .96 0.98 1.00 -0.46 -0.67 0.90 1.00
C6THE3 Aspartate aminotransferase
OS=Glycine max PE=2 SV=1 -0.81 -0.08 1.00 1.00 - 0.01 -0.02 0.92 1.00 -0.42 -0.44 0.93 1.00 -0.33 -0.40 0.87 1.00
M1FPH1
ATP synthase subunit alpha
OS=Glycine max GN=atp1-3 PE=3
SV=1
0.63 0.07 0.99 1.00 -1.74 -2.51 1.00 0.26 -1.82 -2 .17 1.00 0.50 -1.74 -2.51 1.00 0.38
P24459
ATP synthase subunit alpha,
mitochondrial OS=Phaseolus
vulgaris GN=ATPA PE=1 SV=1
0.27 0.02 0.98 1.00 -1.21 -3.50 0.99 0.03 -1.65 -3 .34 1.00 0.05 -0.96 -3.03 1.00 0.21
I1NFS4 ATP synthase subunit beta
OS=Glycine max PE=3 SV=1 0.78 0.08 0 .99 1.00 -0.34 -1.99 1.00 0.51 -0.27 -1.44 1.00 0.84 -0.65 -3.48 0.98 0.11
A4GG90
ATP synthase subunit beta,
chloroplastic OS=Phaseolus
vulgaris GN=atpB PE=3 SV=1
0.01 0.00 0.98 1.00 0.01 0.01 0.93 1.00 -0.09 -0.08 0.97 0.99 0.01 0.01 0.97 0.94
P13917 Basic 7S globulin OS=Glycine max
GN=BG PE=1 SV=2 1.44 0.16 0.97 1.00 -0.30 -1.49 1.00 0.76 -0.28 -1.22 1.00 0.92 -0.72 -3.18 0.99 0.18
T2DP23 Basic 7S globulin OS=Phaseolus
vulgaris PE=2 SV=1 -2.25 -0.21 1.00 0.82 -0.61 -0. 79 0.95 1.00 -0.71 -0.78 0.96 1.00 -0.61 -0.79 0.92 0.99
I1LTJ5 Beta-galactosidase OS=Glycine
max PE=3 SV=1 0.98 0.09 0.99 1.00 0.00 0.00 0.93 1.00 -0.09 -0.09 0.97 0.99 0.00 0.00 0.97 0.95
T2DPW3
Beta-glucosidase 44-like protein
OS=Phaseolus vulgaris PE=2
SV=1
-0.10 -0.01 0.98 1.00 -0.41 -0. 76 0.95 1.00 -0.21 -0.37 0.93 1.00 -0.24 -0.48 0.88 1.00
B0M1A5
Betaine aldehyde dehydrogenase
OS=Glycine max GN=BADH2
PE=2 SV=1
-0.50 -0.05 0.98 1.00 -0.48 -1. 27 1.00 0.86 -0.65 -1.40 1.00 0.86 -0.57 -1.48 1.00 0.81
Q9M507 Beta-ketoacyl-ACP synthetase I-2
OS=Glycine max PE=2 SV=1 1.09 0.11 0 .99 1.00 0.02 0.02 0.94 1.00 -0.06 -0.06 0.98 0.99 0.02 0.02 0.98 0.94
P01060
Bowman-Birk type proteinase
inhibitor 2 OS=Phaseolus vulgaris
GN=BBI PE=1 SV=3
-0.39 -0.04 0.98 1.00 -0.09 -0. 59 0.92 1.00 -0.01 -0.03 0.99 0.99 0.03 0.22 1.00 0.92
O23959
Ca+2-binding EF hand protein
OS=Glycine max GN=GmPM13
PE=2 SV=1
-0.22 -0.02 0.98 1.00 0.10 0.39 1.00 0.96 0.01 0.03 1.00 0.98 0.04 0.13 1.00 0.93
Q39890 Cal modulin OS=Glycine max
GN=SCaM-4 PE=1 SV=1 -1.22 -0.11 1.00 0.96 -0.59 -0.76 0.95 1.00 -0.70 -0 .75 0.96 1.00 -0.59 -0.76 0.91 0.99

S16
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Q93VL8 Calmodulin OS=Phaseolus
vulgaris GN=CaM PE=2 SV=1 0.27 0 .03 0.98 1.00 -0.13 -0.56 0.91 1.00 -0.52 -1.77 1.00 0.69 -0.44 -1.72 1.00 0.71
T2DLJ5
Calnexin-like protein
OS=Phaseolus vulgaris PE=2
SV=1
1.89 0.20 0.94 1.00 -0.45 -2.02 1.00 0.49 -0.32 -1 .30 1.00 0.90 -0.65 -2.71 1.00 0.31
A0A762 Calreticulin-1 OS=Glycine max
GN=Gm crt-1 PE=1 SV=1 1.07 0.11 0.99 1.00 -0.20 -0.85 0.96 1.00 -0.1 3 -0.49 0.93 1.00 -0.08 -0.35 0.88 1.00
K0VRX2
Capsular polysaccharide
biosynthesis protein (Fragment)
OS=Rhizobium sp. Pop5
GN=RCCGEPOP_15776 PE=4
SV=1
-0.24 -0.03 0.98 1.00 -0.01 -0. 01 0.92 1.00 -0.11 -0.10 0.97 1.00 -0.01 -0.01 0.96 0.95
C6TCQ8 Carbonic anhydrase OS=Glycine
max PE=2 SV=1 0.91 0.09 0.99 1.00 0.01 0.01 0.93 1.00 -0.08 -0.08 0.97 0.99 0.01 0.01 0.97 0.95
T2DP18 Cbs domain protein OS=Phaseolus
vulgaris PE=2 SV=1 -1.97 -0.18 1.00 0.84 -1.61 -2. 32 1.00 0.34 -1.71 -1.99 1.00 0.59 -1.61 -2.32 1.00 0.45
L0LT14
Chaperone protein HtpG
OS=Rhizobium tropici CIAT 899
GN=htpG PE=3 SV=1
-0.36 -0.04 0.98 1.00 0.38 0.74 1.00 0.96 0.40 0.72 1.00 0.98 -0.01 -0.01 0.96 0.95
L0LS48
Chemotaxis protein CheW
OS=Rhizobium tropici CIAT 899
GN=RTCIAT899_PC01215 PE=4
SV=1
-1.34 -0.13 1.00 0.92 -0.02 -0. 03 0.92 1.00 -0.11 -0.10 0.97 1.00 -0.02 -0.03 0.95 0.95
T2DNW6
CHP-rich zinc finger protein
OS=Phaseolus vulgaris PE=2
SV=1
0.29 0.03 0.98 1.00 -0.26 -0.37 0.89 1.00 -0.68 -0 .73 0.95 1.00 -0.58 -0.75 0.91 1.00
I1KY39 Citrate synthase OS=Glycine max
PE=3 SV=1 0.41 0.04 0.98 1.00 -0.34 -0.41 0.89 1.00 -0.44 -0 .46 0.93 1.00 -0.34 -0.41 0.88 1.00
I1KTQ4 Ci trate synthase OS=Glycine max
PE=3 SV=1 -1.40 -0.13 1.00 0.93 -0.02 -0. 02 0.92 1.00 -0.11 -0.11 0.96 1.00 -0.02 -0.02 0.96 0.95
I1JIA0 Clathrin heavy chain OS=Glycine
max PE=3 SV=2 -2.85 -0.28 1.0 0 0.88 -0.19 -0.79 0.95 1.00 -0.20 -0.74 0.96 1.00 -0.43 -1.72 1.00 0.71
I1JSP6 Coatomer subunit gamma
OS=Glycine max PE=3 SV=1 -0.89 -0.08 0.99 1.00 - 0.79 -1.04 0.99 0.96 -0.88 -0.99 0.98 0.99 -0.79 -1.04 0.95 0.94
S3HK98
Crp/Fnr family transcriptional
regulator OS=Rhizobium grahamii
CCGE 502
GN=RGCCGE502_06569 PE=4
SV=1
-0.82 -0.08 1.00 1.00 0.34 0.42 1.00 0.96 -0.08 -0.08 0.97 0.99 0.01 0.01 0.97 0.94
C6SXE1 Cyanate hydratase OS=Glycine
max GN=CYN PE=2 SV=1 -0. 46 -0.05 0.98 1.00 -0.13 -0.25 0.89 1.00 -0.03 - 0.04 0.98 0.99 -0.43 -0.78 0.91 0.99
O24323
Cysteine proteinase
OS=Phaseolus vulgaris PE=1
SV=1
0.58 0.06 0.98 1.00 0.78 1.02 1.00 0.97 -0.09 -0.09 0.97 0.99 0.78 1.02 1.00 0.93
T2DP66
Cysteine proteinase
OS=Phaseolus vulgaris PE=2
SV=1
0.00 0.00 0.98 1.00 -0.27 -0.53 0.91 1.00 -0.22 -0 .39 0.93 1.00 0.11 0.25 1.00 0.92
Q8W1A0 Cysteine synthase OS=Glycine
max PE=1 SV=1 1.76 0.17 0.97 1.00 0.16 0.30 1.00 0.96 0.24 0.41 1.00 0.97 -0.94 -1.28 0.99 0.88
A7XTY1
Cytosolic glutathione reductase
OS=Phaseolus vulgaris GN=cGR
PE=1 SV=1
-0.15 -0.02 0.98 1.00 0.23 0.87 1.00 0.97 0.11 0.37 1.00 0.97 -0.16 -0.58 0.89 1.00
F8QXP9 Defensin D1 OS=Phaseolus
vulgaris PE=4 SV=1 -0.78 -0.08 1.00 1.00 0.87 3.47 0.01 0.98 0.68 2.40 0.19 0.99 0.48 1.82 1.00 0.95

S17
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Q41111 Dehydri n OS=Phaseolus vulgaris
PE=1 SV=1 2.81 0.28 0.84 1.00 -0.31 -0.39 0.89 1.00 -0.42 -0 .44 0.93 1.00 -0.31 -0.39 0.87 1.00
Q84RP7
Double-headed trypsin inhibitor
(Fragment) OS=Phaseolus vulgaris
GN=bbi PE=3 SV=1
0.22 0.02 0.98 1.00 -0.99 -1.62 1.00 0.70 -0.59 -0 .95 0.98 1.00 -0.99 -1.62 1.00 0.75
Q8L6E6
Double-headed trypsin inhibitor
(Fragment) OS=Phaseolus vulgaris
GN=bbi20 PE=3 SV=1
-0.23 -0.02 0.98 1.00 -0.52 -1. 42 1.00 0.79 -0.43 -1.06 0.99 0.97 -0.83 -2.05 1.00 0.56
K0GYZ0
DRE-binding protein 2D1
OS=Glycine max GN=DREB2D;1
PE=2 SV=1
-1.05 -0.10 1.00 0.98 0.36 1.50 1.00 0.98 0.26 0.97 1.00 0.98 0.18 0.73 1.00 0.92
T2DN56
Elongation factor 1 beta
OS=Phaseolus vulgaris PE=2
SV=1
-0.26 -0.03 0.98 1.00 0.30 0.53 1.00 0.95 0.55 0.95 1.00 0.98 0.80 1.55 1.00 0.95
A7L3U9
Elongation factor 1-alpha
(Fragment) OS=Phaseolus vulgaris
GN=EF1-a PE=2 SV=1
-0.85 -0.08 0.99 1.00 -0.32 -2. 54 1.00 0.25 -0.25 -1.78 1.00 0.68 -0.52 -3.94 0.97 0.04
C6EVF9
Elongation factor 1-alpha
OS=Glycine max GN=EF-1A PE=2
SV=1
1.88 0.17 0.96 1.00 -0.36 -2.12 1.00 0.44 0.01 0.03 1.00 0.98 -0.48 -2.69 1.00 0.31
T2DPT7
Elongation factor 1-alpha
OS=Phaseolus vulgaris PE=2
SV=1
-1.41 -0.14 1.00 0.91 0.18 1.26 1.00 0.98 -0.09 -0.53 0.93 1.00 0.12 0.80 1.00 0.92
T2DP50
Elongation factor 1-beta
OS=Phaseolus vulgaris PE=2
SV=1
0.97 0.10 0.99 1.00 -0.54 -2.52 1.00 0.26 -0.65 -2 .54 1.00 0.32 -0.77 -3.32 0.99 0.14
T2DM33
Elongation factor 2-like protein
OS=Phaseolus vulgaris PE=2
SV=1
1.03 0.11 0.99 1.00 -0.04 -0.20 0.89 1.00 -0.11 -0 .50 0.93 1.00 -0.32 -1.55 1.00 0.78
O04299
Elongation factor-1 alpha
(Fragment) OS=Glycine max
GN=TefS1 PE=4 SV=1
0.90 0.10 0.99 1.00 -1.92 -2.86 1.00 0.15 -2.00 -2 .44 1.00 0.37 -1.92 -2.86 1.00 0.26
T2DP13
Endo-1314-beta-D-glucanase-like
protein OS=Phaseolus vulgaris
PE=2 SV=1
-1.17 -0.12 1.00 0.95 -0.63 -0. 97 0.98 0.99 -1.05 -1.20 1.00 0.93 -0.96 -1.33 0.99 0.86
K9UTZ9
Epidermis-specific secreted
glycoprotein EP1-like protein
OS=Phaseolus vulgaris PE=2
SV=1
1.41 0.14 0.98 1.00 -0.31 -0.38 0.89 1.00 -0.41 -0 .43 0.93 1.00 -0.31 -0.38 0.87 1.00
Q39856 Epoxide hyd rolase OS=Glycine
max PE=2 SV=1 -4.30 -0.44 1.0 0 0.13 -0.15 -0.82 0.96 1.00 -0.10 -0.51 0.93 1.00 -0.72 -3.23 0.99 0.17
P05088
Erythroagglutinating
phytohemagglutinin OS=Phaseolus
vulgaris GN=DLEC1 PE=1 SV=1
0.67 0.07 0.99 1.00 0.05 1.45 1.00 0.98 -0.02 -0.53 0.93 1.00 0.00 -0.02 0.96 0.95
I1JQD9
Eukaryotic translation initiation
factor 3 subunit C OS=Glycine max
PE=3 SV=1
-2.18 -0.22 1.00 0.82 -0.02 -0. 03 0.92 1.00 -0.12 -0.12 0.96 1.00 -0.02 -0.03 0.95 0.95
I1J582
Eukaryotic translation initiation
factor 3 subunit I OS=Glycine max
PE=3 SV=1
1.10 0.12 0.99 1.00 -0.12 -0.23 0.89 1.00 -0.79 -1 .12 1.00 0.96 -1.20 -1.70 1.00 0.71
C6ZHS4
Eukaryotic translation initiation
factor 5A2 OS=Glycine max PE=2
SV=1
0.39 0.04 0.98 1.00 -0.54 -1.25 1.00 0.87 -0.40 -0 .85 0.97 1.00 -0.43 -1.03 0.95 0.95
K0VF89
Excinuclease ABC subunit B
(Fragment) OS=Rhizobium sp.
Pop5 GN=RCCGEPOP_30134
PE=4 SV=1
-1.06 -0.11 1.00 0.97 0.00 -0.01 0.93 1.00 -0.44 -0.78 0.96 1.00 -0.49 -0.99 0.95 0.96

S18
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
T2DNW9
Fasciclin-like arabinogalactan
protein 1-like protein
OS=Phaseolus vulgaris PE=2
SV=1
0.83 0.09 0.99 1.00 -0.02 -0.02 0.92 1.00 -0.10 -0 .10 0.97 1.00 -0.02 -0.02 0.96 0.95
P80366
Fe(3+)-Zn(2+) purple acid
phosphatase OS=Phaseolus
vulgaris PE=1 SV=2
-1.46 -0.15 1.00 0.88 0.14 1.61 0.95 0.98 0.03 0.33 1.00 0.97 -0.19 -1.98 1.00 0.59
T2DPX5
Ferritin 3 chloroplastic-like protein
OS=Phaseolus vulgaris PE=2
SV=1
0.37 0.03 0.98 1.00 0.00 0.00 0.93 1.00 -0.10 -0.09 0.97 1.00 0.00 0.00 0.97 0.95
C6TNF0 Ferritin OS=Glycine max PE=2
SV=1 2.71 0.30 0.84 1.00 0.01 0.01 0.93 1.00 0.41 0.62 1.00 0.98 -0.24 - 0.33 0.88 0.99
P25699
Ferritin, chloroplastic
OS=Phaseolus vulgaris GN=PFE
PE=1 SV=1
1.32 0.14 0.98 1.00 0.00 0.02 0.93 1.00 -0.30 -1.00 0.99 0.99 -0.16 -0.65 0.89 1.00
D2DWA5
Formate dehydrogenase
OS=Phaseolus vulgaris PE=3
SV=1
2.33 0.22 0.91 1.00 -0.02 -0.22 0.89 1.00 -0.23 -2 .38 1.00 0.39 -0.20 -2.39 1.00 0.42
C6TIW0 Fructose-bisphosphate aldolase
OS=Glycine max PE=1 SV=1 0.85 0.09 0 .99 1.00 -0.53 -1.21 1.00 0.89 -0.25 -0.55 0.93 1.00 -0.53 -1.21 0.98 0.90
C6TMG1 Fructose-bisphosphate aldolase
OS=Glycine max PE=2 SV=1 -0.04 0.00 0.98 1.00 0.0 7 0.28 1.00 0.96 -0.37 -1.12 1.00 0.96 -0.63 -2.00 1.00 0.58
I1M6D5 Fructose-bisphosphate aldolase
OS=Glycine max PE=3 SV=1 0.99 0.11 0 .99 1.00 0.16 0.40 1.00 0.96 -0.01 -0.03 0.99 0.99 0.09 0.21 1.00 0.92
I1JH86 Fructose-bisphosphate aldolase
OS=Glycine max PE=3 SV=1 0.46 0.04 0 .98 1.00 -0.03 -0.12 0.90 1.00 0.12 0.51 1.00 0.97 -0.26 -1.13 0.97 0.92
Q43453
G.max mRNA from stress-induced
gene (H4) OS=Glycine max PE=1
SV=1
1.02 0.09 0.99 1.00 -0.01 -0.01 0.93 1.00 -0.10 -0 .10 0.96 1.00 -0.01 -0.01 0.96 0.95
I1JJP2
Glucose-1-phosphate
adenylyltransferase OS=Glycine
max PE=3 SV=1
-0.43 -0.04 0.98 1.00 -0.81 -1. 58 1.00 0.72 -1.18 -1.73 1.00 0.71 -1.26 -2.10 1.00 0.54
I1K764
Glucose-1-phosphate
adenylyltransferase OS=Glycine
max PE=3 SV=1
0.21 0.02 0.98 1.00 -0.93 -1.26 1.00 0.87 -1.02 -1 .15 1.00 0.95 -0.93 -1.26 0.98 0.89
Q84UT1
Glucose-1-phosphate
adenylyltransferase OS=Phaseolus
vulgaris GN=pvagpS1 PE=2 SV=1
0.11 0.01 0.98 1.00 -0.45 -1.68 1.00 0.67 -0.64 -1 .96 1.00 0.60 -0.83 -2.75 1.00 0.30
A8C8H3
Glutamate decarboxylase
OS=Glycine max GN=GAD PE=1
SV=1
1.46 0.15 0.98 1.00 -0.32 -0.40 0.89 1.00 -0.40 -0 .43 0.93 1.00 -0.32 -0.40 0.87 1.00
I1MV76 Gl utamate dehydrogenase
OS=Glycine max PE=3 SV=2 -2.78 -0.29 1.00 0.89 - 0.03 -0.03 0.92 1.00 -0.14 -0.14 0.96 1.00 -0.03 -0.03 0.95 0.95
O82560
Glutamine synthetase cytosolic
isozyme 2 OS=Glycine max PE=1
SV=1
-0.41 -0.04 0.98 1.00 0.18 0.50 1.00 0.95 -0.20 -0.48 0.93 1.00 0.05 0.15 1.00 0.93
C6TJN5 Glutamine synthetase OS=Glycine
max PE=2 SV=1 0.82 0.09 0.99 1.00 0.05 0.17 1.00 0.98 -0.21 -0.55 0.93 1.00 0.00 0.01 0.97 0.94
P04771
Glutamine synthetase PR-2
OS=Phaseolus vulgaris PE=2
SV=1
2.47 0.27 0.84 1.00 -0.42 -1.25 1.00 0.87 -1.04 -2 .21 1.00 0.48 -2.21 -3.40 0.98 0.13
C6T4A1 Glutathione peroxidase
OS=Glycine max PE=2 SV=1 1.33 0.13 0 .99 1.00 0.00 0.00 0.93 1.00 -0.01 -0.03 0.99 0.99 0.00 0.00 0.97 0.95
H2FH46
Glutathione transferase
OS=Phaseolus vulgaris
GN=GSTF1-1 PE=2 SV=1
-0.69 -0.07 0.99 1.00 -0.43 -1. 85 1.00 0.58 -0.46 -1.70 1.00 0.72 -0.57 -2.35 1.00 0.43

S19
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
B2YDQ6 Gly m Bd 28K allergen (Fragment)
OS=Glycine max PE=4 SV=1 0.75 0.08 0 .99 1.00 -0.26 -0.64 0.93 1.00 -1.29 -1.87 1.00 0.64 -1.35 -2.27 1.00 0.47
T2DLI2
Glyceraldehyde-3-phosphate
dehydrogenase OS=Phaseolus
vulgaris PE=2 SV=1
0.68 0.06 0.99 1.00 -0.02 -0.21 0.89 1.00 -0.09 -0 .81 0.96 1.00 -0.12 -1.28 0.99 0.88
Q9S8B3
GNL-1 alpha SUBUNIT=LECTIN
(Fragment) OS=Phaseolus vulgaris
PE=1 SV=1
1.45 0.14 0.98 1.00 0.00 0.00 0.93 1.00 -0.10 -0.10 0.97 1.00 0.00 0.00 0.97 0.95
Q9XIS6
Granule-bound starch synthase I
OS=Phaseolus vulgaris
GN=pvgbss1 PE=2 SV=1
-0.39 -0.04 0.98 1.00 0.24 0.49 1.00 0.95 -1.18 -1.33 1.00 0.89 -1.09 -1.50 1.00 0.80
Q2N1E0
Group 3 late embryogenesis
abundant protein OS=Phaseolus
vulgaris GN=LEA3 PE=4 SV=1
0.85 0.09 0.99 1.00 0.09 1.37 1.00 0.98 0.14 2.01 0.42 0.99 0.00 -0.06 0. 94 0.96
O24442
Group 4-late embryogenesis
abundant protein PvLEA4-25
(Fragment) OS=Phaseolus vulgaris
GN=Pvlea4-25 PE=2 SV=1
1.02 0.10 0.99 1.00 0.20 0.31 1.00 0.96 -0.66 -0.71 0.95 1.00 -0.25 -0.35 0.88 1.00
T2DM56
GTP-binding nuclear protein Ran-3
OS=Phaseolus vulgaris PE=2
SV=1
0.68 0.07 0.99 1.00 -0.46 -2.54 1.00 0.25 -0.30 -1 .56 1.00 0.79 -0.68 -3.49 0.98 0.11
Q01899
Heat shock 70 kDa protein,
mitochondrial OS=Phaseolus
vulgaris PE=1 SV=1
0.38 0.04 0.98 1.00 -0.24 -0.91 0.97 1.00 -0.14 -0 .50 0.93 1.00 -1.64 -3.81 0.97 0.06
Q7X9E6
Heat shock cognate protein 70
(Fragment) OS=Phaseolus vulgaris
PE=2 SV=1
-0.20 -0.02 0.98 1.00 0.94 1.29 1.00 0.98 0.74 0.91 1.00 0.98 0.00 0.00 0.97 0.95
T2DMX7 Heat-shock protein OS=Phaseolus
vulgaris PE=2 SV=1 0.06 0.01 0.98 1.00 -0.01 -0.01 0.92 1.00 -0.10 -0 .10 0.97 1.00 -0.01 -0.01 0.96 0.95
C6SWE9 Histone H2B OS=Glycine max
PE=2 SV=1 1.41 0.15 0.97 1.00 -0.44 -0.81 0.96 1.00 -0.47 -0 .75 0.96 1.00 -0.88 -1.41 1.00 0.83
I1L934 Histone H4 OS=Glycine max PE=3
SV=1 0.63 0.06 0.99 1.00 0.26 0.37 1.00 0.96 -0.41 -0.44 0.93 1.00 0.01 0.01 0.97 0.94
B9MST3 Histone H4 OS=Glycine max PE=3
SV=1 0.05 0.00 0.98 1.00 -0.14 -0.26 0.89 1.00 0.41 0.80 1.00 0.98 0.65 1.45 1.00 0.95
D6C500 HSP90-2 OS=Glycine max PE=1
SV=1 0.25 0.03 0.98 1.00 -0.12 -0.35 0.89 1.00 0.14 0.39 1.00 0.97 -0.26 -0.73 0.91 1.00
T2DNI1
Hydroxyacylglutathione hydrolase
OS=Phaseolus vulgaris PE=2
SV=1
0.57 0.05 0.98 1.00 0.08 0.28 1.00 0.96 -0.24 -0.72 0.95 1.00 -0.23 -0.77 0.91 0.99
Q9FPW5
IAA-protein conjugate
OS=Phaseolus vulgaris GN=iap1
PE=2 SV=1
0.69 0.07 0.99 1.00 0.02 0.23 1.00 0.97 0.02 0.20 1.00 0.97 -0.10 - 1.15 0.97 0.92
I1JR71 Importin subunit alpha OS=Glycine
max PE=3 SV=1 -0.06 -0.01 0.9 8 1.00 -1.02 -2.47 1.00 0.27 -0.50 -1.26 1.00 0.91 -1.14 -2.66 1.00 0.32
T2DNZ6 In2-1 protein OS=Phaseolus
vulgaris PE=2 SV=1 1.68 0.17 0.96 1.00 -0.11 -0.39 0.89 1.00 -0.33 -1 .01 0.99 0.99 -0.60 -1.90 1.00 0.63
Q43634
Intracellular pathogenesis related
protein OS=Phaseolus vulgaris
GN=Ypr10 PE=3 SV=1
-0.58 -0.05 0.98 1.00 0.19 0.53 1.00 0.95 0.17 0.44 1.00 0.97 0.35 1.02 1.00 0.93
T2DMJ8
Intracellular protease
OS=Phaseolus vulgaris PE=2
SV=1
-0.36 -0.04 0.98 1.00 -0.23 -0. 46 0.90 1.00 -1.41 -1.63 1.00 0.75 -0.98 -1.58 1.00 0.76
A4PIT0
Isoamylase-type starch-
debranching enzyme 3
OS=Phaseolus vulgaris
-0.72 -0.07 0.99 1.00 0.26 0.50 1.00 0.95 -0.54 -0.74 0.96 1.00 0.14 0.26 1.00 0.92

S20
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
GN=PvISA3 PE=2 SV=1
D7F7K7 Lectin OS=Glycine max GN=Le4
PE=4 SV=1 1.86 0.18 0.95 1.00 0.00 0.00 0.93 1.00 -0.09 -0.09 0.97 0.99 0.00 0.00 0.97 0.95
F8QXP7 Legumin OS=Phaseolus vulgaris
PE=2 SV=1 0.75 0.08 0.99 1.00 -0.09 -3.02 1.00 0.11 -0.07 -2 .26 1.00 0.45 -0.18 -5.95 0.95 0.00
P05087
Leucoagglutinating
phytohemagglutinin OS=Phaseolus
vulgaris GN=DLEC2 PE=1 SV=1
2.32 0.24 0.87 1.00 -0.07 -1.86 1.00 0.58 -0.20 -4 .60 0.99 0.00 -0.20 -5.29 0.96 0.00
P15231
Leucoagglutinating
phytohemagglutinin OS=Phaseolus
vulgaris GN=PDLEC2 PE=3 SV=1
2.83 0.28 0.83 1.00 0.14 1.03 1.00 0.97 -0.17 -1.09 0.99 0.97 -0.04 -0.28 0.88 0.99
B3TDK6 Lipoxygenase OS=Glycine max
GN=Lx3 PE=3 SV=1 0.56 0.06 0.99 1.00 -0.07 -0.87 0.97 1.00 -0.23 -2.50 1.00 0.34 -0.34 -4.00 0.97 0.04
B3TDK4 Lipoxygenase OS=Glycine max
PE=3 SV=1 1.72 0.17 0.96 1.00 -0.52 -2.09 1.00 0.46 -0.64 -2 .14 1.00 0.51 -0.70 -2.62 1.00 0.34
K7L7J5 Lipoxygenase OS=Glycine max
PE=3 SV=1 1.38 0.14 0.98 1.00 -0.53 -1.25 1.00 0.87 -0.39 -0 .86 0.97 1.00 -1.16 -2.19 1.00 0.50
B3TDK5 Lipoxygenase OS=Glycine max
PE=3 SV=1 -0.24 -0.02 0.98 1.00 0.02 0.14 0.99 0.98 -0.10 -0.50 0.93 1.00 -0.03 -0.20 0.89 0.98
Q9FQF9 Lipoxygenase OS=Phaseolus
vulgaris PE=1 SV=1 -0.85 -0.08 0.99 1.00 -0.30 -2. 22 1.00 0.39 -0.35 -2.23 1.00 0.46 -0.63 -4.22 0.97 0.02
O24320 Li poxygenase OS=Phaseolus
vulgaris PE=1 SV=2 1.94 0.19 0.95 1.00 -0.55 -1.62 1.00 0.70 -0.50 -1 .31 1.00 0.89 -0.71 -1.97 1.00 0.60
L0LEG7
Major facilitator superfamily (MFS)
transporter OS=Rhizobium tropici
CIAT 899
GN=RTCIAT899_CH01800 PE=4
SV=1
0.03 0.00 0.98 1.00 0.08 0.28 1.00 0.96 -0.15 -0.42 0.93 1.00 -0.64 -1.75 1.00 0.69
I1KDM8 Malate dehydrogenase
OS=Glycine max PE=3 SV=1 2.39 0.26 0 .85 1.00 -0.80 -2.60 1.00 0.23 -1.12 -2.76 1.00 0.23 -1.18 -3.32 0.99 0.14
I1LH25 Malate dehydrogenase
OS=Glycine max PE=3 SV=1 -0.80 -0.09 1.00 1.00 0.0 1 0.02 0.93 1.00 -0.07 -0.07 0.98 0.99 0.01 0.02 0.98 0.94
T2DPK7
Malate dehydrogenase
OS=Phaseolus vulgaris PE=2
SV=1
-2.82 -0.27 1.00 0.86 -0.18 -1. 11 1.00 0.93 -0.43 -2.21 1.00 0.47 -0.48 -2.78 1.00 0.29
I1K5Y5 Malic enzyme OS=Glycine max
PE=3 SV=1 0.91 0.09 0.99 1.00 0.01 0.01 0.93 1.00 -0.08 -0.08 0.97 0.99 0.01 0.01 0.97 0.94
I1MM08 Malic enzyme OS=Glycine max
PE=3 SV=2 2.75 0.27 0.84 1.00 -0.35 -0.58 0.92 1.00 0.05 0.08 1.00 0.97 -0.93 -1.28 0.99 0.88
Q9M7M4
Mannose lectin FRIL (Fragment)
OS=Phaseolus vulgaris PE=2
SV=1
-0.98 -0.09 1.00 1.00 -0.13 -3. 07 1.00 0.10 -0.25 -5.09 0.99 0.00 -0.36 -7.91 0.95 0.00
Q01527 Maturation protein OS=Glycine
max GN=gGmpm9 PE=2 SV=1 -1.72 -0.17 1.00 0.86 - 0.01 -0.01 0.93 1.00 -0.42 -0.45 0.93 1.00 -0.33 -0.41 0.87 1.00
Q71EW8 Methionine synthase OS=Glycine
max PE=1 SV=1 0.10 0.01 0.98 1.00 -0.07 -0.53 0.91 1.00 -0.26 -1.72 1.00 0.71 -0.44 -3.13 0.99 0.19
T2DNY2
Mitochondrial aldehyde
dehydrogenase OS=Phaseolus
vulgaris PE=2 SV=1
-2.79 -0.29 1.00 0.89 -0.02 -0. 03 0.92 1.00 -0.45 -0.47 0.93 1.00 -0.35 -0.44 0.88 1.00
L0LF18
Multidrug and toxin extrusion
(MATE) family efflux pump
OS=Rhizobium tropici CIAT 899
1.44 0.14 0.98 1.00 0.26 0.51 1.00 0.95 -0.54 -0.74 0.96 1.00 -0.37 -0.61 0.89 1.00

S21
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
GN=RTCIAT899_CH02970 PE=4
SV=1
T2DNN6
NADPH-specific isocitrate
dehydrogenase OS=Phaseolus
vulgaris PE=2 SV=1
3.04 0.29 0.83 1.00 -0.77 -1.83 1.00 0.59 -0.63 -1 .38 1.00 0.86 -1.40 -2.67 1.00 0.32
B8R511
NBS-LRR type putative disease
resistance protein CNL-J1
OS=Phaseolus vulgaris PE=4
SV=1
1.09 0.10 0.99 1.00 -0.63 -1.08 1.00 0.94 -0.02 -0 .04 0.99 0.99 -0.27 -0.51 0.88 1.00
D3W146
Non-specific lipid-transfer protein
OS=Phaseolus vulgaris GN=Pha v
3.0101 PE=3 SV=1
-1.22 -0.11 1.00 0.96 0.00 0.01 0.93 1.00 -0.07 -0.63 0.94 1.00 -0.09 -0.95 0.94 0.97
T2DPA0
Nucleoredoxin 1-like protein
OS=Phaseolus vulgaris PE=2
SV=1
1.63 0.17 0.96 1.00 -0.81 -1.60 1.00 0.71 -0.86 -1 .45 1.00 0.83 -1.01 -1.86 1.00 0.65
T2DPE5
Nucleoside diphosphate kinase 1
OS=Phaseolus vulgaris PE=2
SV=1
-1.07 -0.12 1.00 0.95 -0.16 -1. 21 1.00 0.89 -0.10 -0.65 0.95 1.00 -0.38 -2.69 1.00 0.31
T2DN63
Oleosin 16 kDa-like protein
OS=Phaseolus vulgaris PE=2
SV=1
2.64 0.27 0.84 1.00 0.02 0.02 0.94 1.00 -0.07 -0.07 0.98 0.99 0.02 0.02 0.98 0.94
P25985
Pathogenesis-related protein 1
OS=Phaseolus vulgaris PE=1
SV=2
0.02 0.00 0.98 1.00 -0.25 -1.50 1.00 0.76 -0.02 -0 .12 0.96 1.00 -0.58 -3.10 1.00 0.20
I1JKR6 Pectinesterase OS=Glycine max
PE=3 SV=2 -1.88 -0.21 1.00 0.82 0.13 0.25 1.00 0.97 -0.11 -0.18 0.95 1.00 -0.14 -0.27 0.88 0.99
C6SV88 Peptidyl-prolyl cis-trans isomerase
OS=Glycine max PE=2 SV=1 -1.10 -0.11 1.00 0.96 - 1.09 -1.52 1.00 0.75 -1.18 -1.34 1.00 0.88 -0.51 -0.86 0.93 0.98
K7LNW4 Pepti dyl-prolyl cis-trans isomerase
OS=Glycine max PE=3 SV=1 -0.72 -0.08 0.99 1.00 - 0.33 -0.41 0.89 1.00 -0.43 -0.45 0.93 1.00 -0.33 -0.41 0.87 1.00
C6SVD3 Peptidyl-prolyl cis-trans isomerase
OS=Glycine max PE=4 SV=1 -0.03 0.00 0.98 1.00 - 0.92 -2.00 1.00 0.50 -1.47 -2.16 1.00 0.50 -1.29 -2.44 1.00 0.40
Q41119
Peptidyl-prolyl cis-trans isomerase
OS=Phaseolus vulgaris GN=Cyp
PE=1 SV=1
1.59 0.14 0.98 1.00 -0.19 -2.62 1.00 0.22 -0.40 -4 .63 0.99 0.00 -0.37 -4.91 0.96 0.00
Q9FE12
Peroxiredoxin OS=Phaseolus
vulgaris GN=2-Cys PRx PE=1
SV=1
0.02 0.00 0.98 1.00 0.00 0.01 0.93 1.00 -0.32 -0.68 0.95 1.00 -0.35 -0.83 0.92 0.99
B0M199
Peroxisomal fatty acid beta-
oxidation multifunctional protein
OS=Glycine max PE=1 SV=1
-1.82 -0.19 1.00 0.83 0.00 0.00 0.93 1.00 -0.09 -0.09 0.97 0.99 0.00 0.00 0.97 0.95
B0M197
Peroxisomal voltage-dependent
anion-selective channel protein
OS=Glycine max PE=2 SV=1
-2.01 -0.20 1.00 0.83 0.01 0.01 0.93 1.00 -0.08 -0.08 0.97 0.99 0.01 0.01 0.97 0.94
P80463 Phaseolin OS=Phaseolus lunatus
GN=PHA PE=1 SV=2 -0.10 -0.01 0.98 1.00 0.01 0.01 0.93 1.00 -0.08 -0.08 0.97 0.99 0.34 0.41 1.00 0.91
Q43632 Phaseoli n OS=Phaseolus vulgaris
GN=Phs PE=2 SV=1 0.37 0.03 0.98 1.00 -0.14 - 4.98 0.99 0.00 -0.11 -3.66 1.00 0.02 -0.15 -5.56 0.95 0.00
Q43633 Phaseoli n OS=Phaseolus vulgaris
GN=Phs PE=2 SV=1 0.30 0.03 0.98 1.00 -0.07 - 1.77 1.00 0.63 -0.09 -2.17 1.00 0.49 -0.06 -1.62 1.00 0.75
P07219
Phaseolin, alpha-type
OS=Phaseolus vulgaris PE=1
SV=1
2.41 0.24 0.88 1.00 -0.19 -5.91 0.98 0.00 -0.10 -2 .79 1.00 0.22 -0.17 -5.38 0.96 0.00
P02853
Phaseolin, beta-type
OS=Phaseolus vulgaris PE=1
SV=2
0.31 0.03 0.98 1.00 -0.09 -3.23 0.99 0.07 -0.06 -1 .87 1.00 0.64 -0.12 -4.08 0.97 0.03

S22
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Q9AU12
Phosphoenolpyruvate carboxylase
OS=Phaseolus vulgaris PE=1
SV=1
-0.17 -0.02 0.98 1.00 0.01 0.01 0.93 1.00 -0.41 -0.44 0.93 1.00 -0.33 -0.40 0.87 1.00
I1MJC6 Phosphoglycerate kinase
OS=Glycine max PE=3 SV=1 0.10 0.01 0 .98 1.00 -0.19 -0.44 0.90 1.00 -1.62 -1.89 1.00 0.63 -0.43 -0.93 0.94 0.97
I1MJC7 Phosphoglycerate kinase
OS=Glycine max PE=3 SV=1 1.58 0.17 0 .96 1.00 -0.11 -0.45 0.90 1.00 -0.18 -0.65 0.95 1.00 -0.42 -1.62 1.00 0.75
I1LWR4 Phosphorylase OS=Glycine max
PE=3 SV=1 -2.66 -0.27 1.00 0.87 -0.04 -0. 29 0.89 1.00 -0.09 -0.64 0.94 1.00 -0.44 -3.31 0.99 0.15
T2DN52
Phytohemagglutinin
OS=Phaseolus vulgaris PE=2
SV=1
0.85 0.08 0.99 1.00 -0.47 -1.60 1.00 0.71 -0.84 -2 .15 1.00 0.50 -0.79 -2.38 1.00 0.42
Q43628
Phytohemagglutinin
OS=Phaseolus vulgaris PE=4
SV=1
0.36 0.04 0.98 1.00 -0.11 -2.03 1.00 0.49 -0.21 -3 .38 1.00 0.05 -0.23 -4.32 0.97 0.02
T2DNK2
Plasminogen activator inhibitor 1
RNA-binding protein
OS=Phaseolus vulgaris PE=2
SV=1
0.68 0.07 0.99 1.00 0.79 1.07 1.00 0.97 0.41 0.46 1.00 0.97 0.02 0.02 0.98 0.94
Q9SWB4
Poly [ADP-ribose] polymerase 3
OS=Glycine max GN=PARP3
PE=2 SV=1
0.76 0.07 0.99 1.00 -0.03 -0.15 0.89 1.00 -0.06 -0 .28 0.93 1.00 -0.31 -1.66 1.00 0.73
T2DPD6 Profilin OS=Phaseolus vulgaris
PE=2 SV=1 -2.21 -0.24 1.00 0.83 -0.26 -0. 72 0.94 1.00 -0.75 -1.55 1.00 0.79 -0.72 -1.74 1.00 0.70
P49231 Profilin-1 OS=Phaseolus vulgaris
PE=1 SV=1 1.41 0.17 0.97 1.00 -0.63 -1.08 0.99 0.95 -0.80 -1 .12 1.00 0.96 -1.20 -1.70 1.00 0.71
I1ME45 Pro line iminopeptidase
OS=Glycine max PE=3 SV=1 1.10 0.12 0 .99 1.00 0.08 0.20 1.00 0.97 -0.16 -0.33 0.93 1.00 -0.73 -1.44 1.00 0.82
Q41125
Proline-rich 14 kDa protein
OS=Phaseolus vulgaris PE=2
SV=1
2.01 0.19 0.95 1.00 0.26 0.36 1.00 0.96 -0.41 -0.42 0.93 1.00 -0.32 -0.38 0.87 1.00
C6TNX9 Proteasome subunit alpha type
OS=Glycine max PE=2 SV=1 0.21 0.02 0 .98 1.00 -0.46 -1.87 1.00 0.57 -0.83 -2.61 1.00 0.29 -0.80 -2.93 1.00 0.24
C6TH59 Proteasome subunit alpha type
OS=Glycine max PE=2 SV=1 0.70 0.07 0 .99 1.00 -0.62 -2.10 1.00 0.45 -0.55 -1.66 1.00 0.74 -0.88 -2.68 1.00 0.32
C6TG97 Proteasome subunit alpha type
OS=Glycine max PE=2 SV=1 -0.34 -0.04 0.98 1.00 0.4 3 0.79 1.00 0.96 -0.05 -0.07 0.97 0.99 0.16 0.28 1.00 0.92
C6TAW4 Proteasome subunit alpha type
OS=Glycine max PE=2 SV=1 -1.45 -0.15 1.00 0.89 - 0.12 -0.24 0.89 1.00 -0.03 -0.06 0.98 0.99 -0.43 -0.79 0.92 0.99
I1JBH2 Proteasome subunit alpha type
OS=Glycine max PE=3 SV=1 1.06 0.10 0 .99 1.00 -0.39 -1.24 1.00 0.88 -0.53 -1.39 1.00 0.86 -0.94 -2.46 1.00 0.39
I1L3S2 Proteasome subunit alpha type
OS=Glycine max PE=3 SV=1 0.57 0.06 0 .99 1.00 -0.32 -0.39 0.89 1.00 -0.42 -0.44 0.93 1.00 -0.01 -0.01 0.96 0.95
I1MN39 Proteasome subunit alpha type
OS=Glycine max PE=3 SV=1 -1.30 -0.13 1.00 0.94 - 0.44 -0.79 0.95 1.00 -0.23 -0.39 0.93 1.00 -0.64 -1.08 0.96 0.93
C6SWQ4 Proteasome subunit beta type
OS=Glycine max PE=2 SV=1 -0.35 -0.04 0.98 1.00 - 0.76 -1.94 1.00 0.53 -0.90 -1.92 1.00 0.62 -0.87 -2.19 1.00 0.50
C6TLJ9 Proteasome subunit beta type
OS=Glycine max PE=2 SV=1 -1.30 -0.14 1.00 0.92 - 0.45 -0.84 0.96 1.00 -0.82 -1.18 1.00 0.94 -0.64 -1.13 0.97 0.92
C6TBS6 Proteasome subunit beta type
OS=Glycine max PE=2 SV=1 2.19 0.22 0 .92 1.00 0.05 0.05 0.95 1.00 -0.03 -0.03 0.99 0.99 0.05 0.05 1.00 0.94
T2DNM6 Proteasome subunit beta type-6-
like protein OS=Phaseolus vulgaris - 2.02 -0.20 1.00 0.83 0.28 0.76 1.00 0.96 0.40 1.00 1.00 0.98 -0.39 -0.89 0.93 0.98

S23
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
PE=2 SV=1
A9CPA7
Protein disulfide isomerase family
OS=Glycine max GN=GmPDIM
PE=2 SV=1
0.03 0.00 0.98 1.00 -0.58 -0.73 0.94 1.00 0.91 1.53 0.82 0.99 -0.58 -0.73 0.91 1.00
B1Q2X4
Protein disulfide isomerase
OS=Glycine max GN=PDIL-1
PE=1 SV=1
-0.73 -0.08 0.99 1.00 -0.01 -0. 01 0.92 1.00 -0.11 -0.10 0.97 1.00 -0.01 -0.01 0.96 0.95
O24319
Purple acid phosphatase
OS=Phaseolus vulgaris GN=pap
PE=1 SV=1
0.31 0.03 0.98 1.00 0.27 3.43 0.01 0.98 0.25 2.75 0.10 0.99 0.05 0.60 1.00 0.91
N6V5Q7
Putative fructose transport system
kinase OS=Rhizobium freirei PRF
81 GN=RHSP_19647 PE=4 SV=1
0.89 0.09 0.99 1.00 0.02 0.02 0.94 1.00 -0.08 -0.08 0.97 0.99 0.02 0.02 0.98 0.94
C6F117 Putative ribosomal protein S15
OS=Glycine max PE=2 SV=1 -2.43 -0.28 1.00 0.88 - 0.89 -2.42 1.00 0.30 -0.83 -1.99 1.00 0.58 -0.80 -2.28 1.00 0.47
G3E7M5
Putative rubisco subunit binding-
protein alpha subunit OS=Glycine
max PE=2 SV=1
-0.11 -0.01 0.98 1.00 0.31 0.55 1.00 0.95 0.20 0.31 1.00 0.97 -0.21 -0.32 0.88 0.99
C6TD94
Putative uncharacterized protein
(Fragment) OS=Glycine max PE=1
SV=1
0.42 0.04 0.98 1.00 -0.51 -1.31 1.00 0.84 -0.94 -1 .81 1.00 0.67 -1.15 -2.41 1.00 0.41
C6TD18
Putative uncharacterized protein
(Fragment) OS=Glycine max PE=2
SV=1
-1.07 -0.11 1.00 0.98 -0.35 -0. 84 0.96 1.00 -0.94 -1.56 1.00 0.79 -0.90 -1.78 1.00 0.68
C6SX09
Putative uncharacterized protein
(Fragment) OS=Glycine max PE=2
SV=1
-0.51 -0.05 0.98 1.00 -1.21 -1. 67 1.00 0.68 -1.30 -1.47 1.00 0.83 -1.21 -1.67 1.00 0.73
C6TNT7
Putative uncharacterized protein
(Fragment) OS=Glycine max PE=2
SV=1
-0.22 -0.02 0.98 1.00 -0.18 -0. 31 0.89 1.00 -1.04 -1.17 1.00 0.94 -0.96 -1.30 0.99 0.87
C6T6K1
Putative uncharacterized protein
(Fragment) OS=Glycine max PE=2
SV=1
0.06 0.01 0.98 1.00 -0.89 -1.78 1.00 0.62 -0.32 -0 .67 0.95 1.00 -1.66 -2.45 1.00 0.40
C6T7C4
Putative uncharacterized protein
(Fragment) OS=Glycine max PE=2
SV=1
-1.21 -0.14 1.00 0.92 0.32 0.39 1.00 0.96 -0.10 -0.10 0.97 1.00 -0.02 -0.02 0.96 0.95
C6TIJ2
Putative uncharacterized protein
(Fragment) OS=Glycine max PE=2
SV=1
-0.97 -0.10 1.00 0.99 0.02 0.02 0.94 1.00 -0.09 -0.08 0.97 0.99 0.02 0.02 0.98 0.94
C6TGF3
Putative uncharacterized protein
(Fragment) OS=Glycine max PE=2
SV=1
2.02 0.20 0.94 1.00 0.02 0.02 0.93 1.00 -0.07 -0.07 0.98 0.99 0.02 0.02 0.98 0.94
C6TJT5 Putative uncharacterized protein
OS=Glycine max PE=1 SV=1 0.69 0.06 0 .99 1.00 -0.63 -1.93 1.00 0.54 -0.65 -1.71 1.00 0.72 -0.77 -2.24 1.00 0.48
C6TID1 Putative uncharacterized protein
OS=Glycine max PE=1 SV=1 -0.94 -0.10 1.00 0.99 - 0.37 -0.74 0.94 1.00 -1.42 -1.61 1.00 0.76 -0.54 -1.03 0.95 0.95
C6SWE2 Putative uncharacterized protein
OS=Glycine max PE=1 SV=1 -1.15 -0.13 1.00 0.94 - 0.57 -0.73 0.94 1.00 -0.65 -0.70 0.95 1.00 -0.57 -0.73 0.91 1.00
C6SW15 Putati ve uncharacterized protein
OS=Glycine max PE=1 SV=1 0.56 0.06 0 .98 1.00 -0.44 -0.64 0.93 1.00 -0.37 -0.48 0.93 1.00 -0.77 -1.00 0.95 0.95
C6TFA0 Putative uncharacterized protein
OS=Glycine max PE=1 SV=1 1.58 0.17 0 .96 1.00 0.00 0.00 0.93 1.00 -0.10 -0.10 0.97 1.00 0.00 0.00 0.97 0.95
C6T7Z3 Putative uncharacterized protein
OS=Glycine max PE=1 SV=1 1.84 0.18 0 .95 1.00 0.00 0.00 0.93 1.00 -0.09 -0.09 0.97 0.99 0.00 0.00 0.97 0.95

S24
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
C6SY23 Putative uncharacterized protein
OS=Glycine max PE=1 SV=1 2.78 0.27 0 .84 1.00 0.02 0.02 0.94 1.00 -0.08 -0.08 0.97 0.99 0.02 0.02 0.98 0.94
C6TBD8 Putative uncharacterized protein
OS=Glycine max PE=1 SV=1 0.25 0.02 0 .98 1.00 0.00 -0.01 0.93 1.00 -0.03 -0.05 0.98 0.99 0.0 0 -0.01 0.96 0.95
C6TI56 Putative uncharacterized protein
OS=Glycine max PE=1 SV=1 0.00 0.00 0 .98 1.00 0.35 0.58 1.00 0.96 0.39 0.59 1.00 0.97 0.20 0.32 1.00 0.91
C6TGA8 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.98 0.10 0 .99 1.00 -0.40 -2.27 1.00 0.37 -1.63 -5.06 0.99 0.00 -0.93 -4.44 0.96 0.01
C6T5N1 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.36 -0.04 0.98 1.00 - 0.57 -1.52 1.00 0.75 -1.00 -1.95 1.00 0.60 -1.40 -2.72 1.00 0.30
C6TB70 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 2.01 0.19 0 .95 1.00 -0.07 -0.73 0.94 1.00 -0.23 -1.92 1.00 0.62 -0.18 -1.78 1.00 0.68
C6SZ83 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.78 -0.09 1.00 1.00 - 0.72 -1.53 1.00 0.74 -1.25 -1.84 1.00 0.66 -1.33 -2.26 1.00 0.47
C6T898 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.53 -0.16 1.00 0.87 - 0.47 -2.55 1.00 0.24 -0.36 -1.76 1.00 0.69 -0.51 -2.76 1.00 0.29
C6TAX1 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.10 -0.01 0.98 1.00 0.2 0 0.54 1.00 0.95 -1.21 -1.75 1.00 0.70 -0.01 -0.02 0.96 0.95
C6T7T2 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 1.51 0.16 0 .97 1.00 -0.07 -0.46 0.90 1.00 -0.32 -1.72 1.00 0.71 -0.34 -2.04 1.00 0.57
C6SVR5 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.75 0.07 0 .99 1.00 -0.32 -1.06 0.99 0.95 -0.59 -1.53 1.00 0.80 -0.38 -1.22 0.98 0.90
C6SVX0 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.28 -0.14 1.00 0.90 - 0.86 -1.53 1.00 0.74 -1.03 -1.49 1.00 0.82 -1.43 -2.09 1.00 0.54
C6TNT8 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.86 -0.17 1.00 0.86 0.0 7 0.26 1.00 0.97 -0.53 -1.44 1.00 0.84 -0.60 -1.86 1.00 0.65
C6TCF1 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.97 -0.09 1.00 0.99 - 0.73 -1.41 1.00 0.80 -0.77 -1.26 1.00 0.91 -1.18 -1.93 1.00 0.62
C6TDL5 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.33 -0.03 0.98 1.00 - 0.25 -0.69 0.94 1.00 -0.58 -1.25 1.00 0.92 -0.98 -2.13 1.00 0.53
C6SWE3 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.07 0.01 0 .98 1.00 -0.94 -1.29 1.00 0.85 -1.02 -1.19 1.00 0.94 -0.94 -1.29 0.99 0.87
C6SZK1 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -2.57 -0.26 1.00 0.86 - 0.98 -1.29 1.00 0.85 -1.07 -1.17 1.00 0.94 -0.98 -1.29 0.99 0.87
C6SVH1 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 1.20 0.15 0 .98 1.00 0.27 0.52 1.00 0.95 -1.01 -1.17 1.00 0.94 0.27 0.52 1.00 0.91
C6SYI8 Putati ve uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.65 -0.18 1.00 0.84 - 0.24 -0.61 0.92 1.00 -0.57 -1.16 1.00 0.94 -0.67 -1.50 1.00 0.80
C6T4R2 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.10 -0.01 0.98 1.00 - 0.60 -0.90 0.97 1.00 -1.00 -1.15 1.00 0.95 -0.92 -1.26 0.98 0.89
C6T116 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.32 -0.03 0.98 1.00 - 0.01 -0.02 0.92 1.00 -0.69 -1.11 1.00 0.96 -0.48 -0.97 0.94 0.96
C6TGZ9 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 2.15 0.20 0 .94 1.00 -0.26 -1.01 0.99 0.97 -0.32 -1.10 0.99 0.96 -0.73 -2.48 1.00 0.39
C6TDL9 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -4.10 -0.44 1.00 0.12 - 0.02 -0.03 0.92 1.00 -0.91 -1.00 0.99 0.99 -0.48 -0.70 0.90 1.00
C6SX72 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.49 -0.16 1.00 0.87 - 0.35 -0.83 0.96 1.00 -0.49 -0.97 0.98 1.00 -0.45 -1.04 0.95 0.95
C6TK38 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.14 -0.11 1.00 0.97 - 0.77 -1.03 0.99 0.97 -0.86 -0.97 0.98 1.00 -0.77 -1.03 0.95 0.95

S25
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
C6TB24 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 1.58 0.17 0 .97 1.00 -0.77 -1.02 0.99 0.97 -0.86 -0.94 0.98 1.00 -0.77 -1.02 0.95 0.95
C6TBI1 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.57 0.06 0 .99 1.00 0.08 0.20 1.00 0.97 -0.41 -0.81 0.96 1.00 -0.65 -1.36 1.00 0.85
C6THU7 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.86 -0.09 1.00 1.00 - 0.60 -0.78 0.95 1.00 -0.69 -0.76 0.96 1.00 -0.60 -0.78 0.91 0.99
C6TAZ2 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.17 -0.13 1.00 0.94 - 0.60 -0.75 0.95 1.00 -0.69 -0.74 0.96 1.00 -0.60 -0.75 0.91 1.00
C6THG4 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.55 0.07 0 .99 1.00 0.22 0.34 1.00 0.96 -0.64 -0.70 0.95 1.00 -0.54 -0.69 0.90 1.00
C6TJB9 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 1.35 0.14 0 .98 1.00 0.00 0.01 0.93 1.00 -0.35 -0.55 0.93 1.00 -0.14 -0.26 0.88 0.99
C6TMQ9 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 1.05 0.11 0 .99 1.00 -0.07 -0.17 0.89 1.00 -0.24 -0.51 0.93 1.00 -0.38 -0.87 0.93 0.98
C6SVG3 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.54 -0.06 0.98 1.00 - 0.34 -0.41 0.89 1.00 -0.43 -0.45 0.93 1.00 -0.34 -0.41 0.87 1.00
C6TNC2 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.50 0.05 0 .98 1.00 -0.33 -0.40 0.89 1.00 -0.43 -0.44 0.93 1.00 -0.33 -0.40 0.87 1.00
C6TMV0 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.38 0.04 0 .98 1.00 -0.32 -0.39 0.89 1.00 -0.40 -0.42 0.93 1.00 -0.32 -0.39 0.87 1.00
C6SVZ7 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 1.40 0.14 0 .98 1.00 -0.31 -0.39 0.89 1.00 -0.38 -0.41 0.93 1.00 -0.31 -0.39 0.87 1.00
C6TKP4 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.14 -0.01 0.98 1.00 - 0.21 -0.65 0.93 1.00 -0.14 -0.40 0.93 1.00 -0.21 -0.65 0.89 1.00
C6TG44 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.63 -0.07 0.99 1.00 - 0.38 -0.62 0.92 1.00 -0.22 -0.34 0.93 1.00 -0.95 -1.30 0.99 0.87
C6TA60 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.65 0.06 0 .99 1.00 -0.15 -0.26 0.89 1.00 -0.20 -0.31 0.93 1.00 -0.36 -0.59 0.89 1.00
C6SVZ3 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 1.74 0.18 0 .96 1.00 -0.12 -0.43 0.90 1.00 -0.09 -0.28 0.93 1.00 -0.63 -1.86 1.00 0.64
C6T8Y7 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.27 -0.12 1.00 0.94 - 0.01 -0.01 0.92 1.00 -0.12 -0.12 0.96 1.00 -0.01 -0.01 0.96 0.95
C6TIR2 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 2.65 0.27 0 .84 1.00 -0.02 -0.13 0.90 1.00 -0.02 -0.12 0.96 1.00 -0.15 -0.86 0.93 0.98
C6TCN9 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -2.80 -0.28 1.00 0.88 0.0 0 0.00 0.93 1.00 -0.11 -0.11 0.96 1.00 0.00 0.00 0.97 0.95
C6TLQ4 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.79 0.07 0 .99 1.00 -0.01 -0.01 0.93 1.00 -0.11 -0.11 0.96 1.00 -0.01 -0.01 0.96 0.95
C6TEA4 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.26 -0.12 1.00 0.95 0.3 3 0.40 1.00 0.96 -0.09 -0.09 0.97 0.99 0.00 0.00 0.96 0.95
C6TL04 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.06 0.01 0 .98 1.00 0.01 0.01 0.93 1.00 -0.09 -0.09 0.97 0.99 0.01 0.01 0.97 0.94
C6TGG3 Putati ve uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.56 -0.05 0.98 1.00 0.0 0 0.00 0.93 1.00 -0.09 -0.09 0.97 0.99 0.00 0.00 0.96 0.95
C6SXV0 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 2.99 0.29 0 .83 1.00 0.01 0.01 0.93 1.00 -0.09 -0.09 0.97 0.99 0.01 0.01 0.97 0.94
C6TGR8 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.42 -0.05 0.98 1.00 0.3 4 0.41 1.00 0.96 -0.08 -0.08 0.97 0.99 0.00 0.00 0.97 0.95
C6TCC4 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.33 -0.03 0.98 1.00 0.0 1 0.01 0.93 1.00 -0.08 -0.08 0.97 0.99 0.01 0.01 0.97 0.94

S26
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
C6SZF9 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.11 -0.01 0.98 1.00 0.0 1 0.01 0.93 1.00 -0.07 -0.07 0.97 0.99 0.01 0.01 0.97 0.94
C6THN1 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.47 0.05 0 .98 1.00 0.00 0.00 0.93 1.00 -0.03 -0.05 0.98 0.99 -0.88 -1.39 1.00 0.84
C6TK81 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.65 -0.17 1.00 0.86 - 0.17 -0.41 0.89 1.00 0.01 0.03 1.0 0 0.98 0.23 0.60 1.00 0.91
C6SVF4 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 1.09 0.11 0 .99 1.00 -0.13 -0.36 0.89 1.00 0.05 0.13 1.00 0.97 -0.45 -1.12 0.97 0.93
C6SZ11 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 1.03 0.11 0 .99 1.00 -1.53 -2.68 1.00 0.20 0.05 0.13 1.00 0.97 -1.86 -2.84 1.00 0.27
C6TEK4 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.03 0.00 0 .98 1.00 0.01 0.03 0.94 1.00 0.07 0.16 1.00 0.97 -1.09 -1.79 1.00 0.67
C6T9C2 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.63 -0.16 1.00 0.87 0.1 2 0.42 1.00 0.96 0.09 0.28 1.00 0.97 0.09 0.29 1.00 0.91
C6TA32 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.74 -0.17 1.00 0.86 0.3 0 0.36 1.00 0.96 0.37 0.42 1.00 0.97 -0.04 -0.04 0.95 0.95
C6TAN1 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.81 -0.08 0.99 1.00 - 0.03 -0.03 0.92 1.00 0.37 0.42 1.0 0 0.97 -0.03 -0.03 0.95 0.95
C6T7U0 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.08 -0.01 0.98 1.00 0.2 4 0.49 1.00 0.95 0.26 0.49 1.00 0.97 -0.76 -1.19 0.97 0.91
C6SXU0 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 1.63 0.17 0 .96 1.00 -0.13 -0.25 0.89 1.00 0.28 0.52 1.00 0.97 -1.08 -1.50 1.00 0.80
C6TM35 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.49 -0.17 1.00 0.86 0.7 6 1.17 1.00 0.98 0.41 0.54 1.00 0.97 0.26 0.37 1.00 0.91
C6SWN7 Putati ve uncharacterized protein
OS=Glycine max PE=2 SV=1 1.47 0.15 0 .97 1.00 -0.31 -0.38 0.89 1.00 0.41 0.55 1.00 0.97 0.00 0.01 0.97 0.95
C6TEC2 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 0.31 0.03 0 .98 1.00 0.10 0.31 1.00 0.96 0.19 0.57 1.00 0.97 0.22 0.72 1.00 0.92
C6TL09 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.51 -0.05 0.98 1.00 - 0.06 -0.17 0.89 1.00 0.22 0.60 1.0 0 0.98 -0.12 -0.34 0.88 1.00
C6T498 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -2.03 -0.21 1.00 0.82 0.5 7 1.16 1.00 0.98 0.40 0.73 1.00 0.98 0.49 1.00 1.00 0.93
C6TKW9 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.56 -0.06 0.99 1.00 - 0.08 -0.28 0.89 1.00 0.22 0.79 1.0 0 0.98 0.00 -0.02 0.96 0.95
C6T8X3 Putative uncharacterized protein
OS=Glycine max PE=2 SV=1 -2.37 -0.21 1.00 0.82 0.2 9 1.22 1.00 0.98 0.26 0.97 1.00 0.98 -0.03 -0.12 0.91 0.97
C6SVE3 Putative uncharacterized protein
OS=Glycine max PE=4 SV=1 2.19 0.25 0 .87 1.00 -0.21 -0.46 0.90 1.00 -0.24 -0.47 0.93 1.00 -0.21 -0.46 0.88 1.00
C6SVK9 Putative uncharacterized protein
OS=Glycine max PE=4 SV=1 0.83 0.08 0 .99 1.00 0.00 0.00 0.93 1.00 -0.10 -0.09 0.97 0.99 0.00 0.00 0.97 0.95
C6T0H2 Putative uncharacterized protein
OS=Glycine max PE=4 SV=1 1.54 0.16 0 .97 1.00 -0.28 -0.87 0.97 1.00 0.00 0.00 1.00 0.98 -0.28 -0.87 0.93 0.98
C6SW37 Putati ve uncharacterized protein
OS=Glycine max PE=4 SV=1 1.49 0.15 0 .97 1.00 0.00 0.00 0.93 1.00 0.41 0.47 1.00 0.97 0.00 0.00 0.97 0.95
E9NZS9
Putative uncharacterized protein
OS=Phaseolus vulgaris PE=4
SV=1
0.79 0.08 0.99 1.00 0.00 0.00 0.93 1.00 0.30 0.64 1.00 0.98 -0.23 - 0.47 0.88 1.00
Q41108 Pv42p OS=Phaseol us vulgaris
PE=2 SV=1 -1.09 -0.11 1.00 0.97 0.04 0.32 1.00 0.96 0.02 0.11 1.00 0.97 -0.11 -0.82 0.92 0.99

S27
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
O24439 PvLEA-18 OS=Phaseolus vulgaris
GN=Pvlea-18 PE=4 SV=1 -0.32 -0.03 0.98 1.00 0.13 0.67 1.00 0.96 0.12 0.58 1.00 0.97 0.16 0.85 1.00 0.92
Q9FT25
Pyridoxal biosynthesis protein
PDX1 OS=Phaseolus vulgaris
GN=PDX1 PE=2 SV=1
0.95 0.10 0.99 1.00 0.34 0.42 1.00 0.96 0.74 0.92 1.00 0.98 0.95 1.30 1.00 0.94
I1N5N0 Pyruvate kinase OS=Glycine max
PE=3 SV=1 -1.03 -0.11 1.00 0.97 -0.89 -1. 42 1.00 0.80 -1.32 -1.53 1.00 0.80 -1.22 -1.74 1.00 0.70
I1JPV5 Pyruvate kinase OS=Glycine max
PE=3 SV=1 -0.20 -0.02 0.98 1.00 -0.33 -0. 40 0.89 1.00 -0.41 -0.43 0.93 1.00 0.01 0.01 0.97 0.94
T2DLS9
Quinone-oxidoreductase-like
protein OS=Phaseolus vulgaris
PE=2 SV=1
1.27 0.13 0.98 1.00 -0.43 -0.92 0.97 1.00 -1.61 -1 .89 1.00 0.63 -1.52 -2.20 1.00 0.50
B7T1N8 Rack OS=Phaseolus vulgaris
GN=RACK PE=2 SV=1 -1.28 -0.13 1.00 0.93 0.05 0.27 1.00 0.97 0.04 0.20 1.00 0.97 -0.39 -1.97 1.00 0.60
C6T0K5 Reticulon-like protein (Fragment)
OS=Glycine max PE=2 SV=1 -0.89 -0.09 1.00 1.00 0.5 8 1.25 1.00 0.98 0.10 0.17 1.00 0.97 0.00 -0.01 0.96 0.95
C6TKL2 Reticulon-like protein OS=Glycine
max PE=2 SV=1 -1.70 -0.16 1.0 0 0.87 -0.02 -0.03 0.92 1.00 -0.11 -0.11 0.96 1.00 -0.02 -0.03 0.95 0.95
C6SV78 Ribosomal protein L15 OS=Glycine
max PE=2 SV=1 -0.57 -0.06 0.9 9 1.00 -0.47 -1.06 0.99 0.95 -0.33 -0.70 0.95 1.00 -0.47 -1.06 0.96 0.94
E9NZT0
Ribosomal protein L19
OS=Phaseolus vulgaris PE=3
SV=1
2.66 0.29 0.83 1.00 0.01 0.01 0.93 1.00 0.41 0.48 1.00 0.97 0.01 0.01 0.97 0.94
C6SZN4 Ribosomal protein L37 OS=Glycine
max PE=3 SV=1 0.52 0.05 0.98 1.00 1.22 1.74 0.81 0.99 1.00 1.32 0.94 0.99 0.97 1.33 1.00 0.94
C6SVL1 Ribosomal protein OS=Glycine
max PE=2 SV=1 1.25 0.12 0.99 1.00 -0.01 -0.02 0.92 1.00 0.03 0.04 1.00 0.98 -0.38 -0.63 0.89 1.00
K0VSU2
Secretion protein HlyD family
protein OS=Rhizobium sp. Pop5
GN=RCCGEPOP_14127 PE=4
SV=1
-0.07 -0.01 0.98 1.00 0.01 0.01 0.93 1.00 0.40 0.47 1.00 0.97 0.01 0.01 0.97 0.94
C6K8D1
Seed biotinylated protein 68 kDa
isoform OS=Glycine max PE=2
SV=1
1.38 0.14 0.98 1.00 0.14 0.37 1.00 0.96 0.19 0.47 1.00 0.97 0.01 0.03 0.98 0.94
Q9XER5
Seed maturation protein PM22
OS=Glycine max GN=PM22 PE=2
SV=1
0.94 0.10 0.99 1.00 -0.20 -0.61 0.92 1.00 -0.21 -0 .58 0.94 1.00 -0.26 -0.80 0.92 0.99
Q9XES8
Seed maturation protein PM28
OS=Glycine max GN=PM28 PE=4
SV=1
0.44 0.05 0.98 1.00 -0.02 -0.03 0.92 1.00 -0.12 -0 .12 0.96 1.00 -0.02 -0.03 0.95 0.95
Q9XET0
Seed maturation protein PM30
OS=Glycine max GN=PM30 PE=2
SV=1
0.30 0.03 0.98 1.00 -0.13 -0.80 0.95 1.00 0.08 0.48 1.00 0.97 -0.61 -3.19 0.99 0.17
C6THM7 Seri ne hydroxymethyltransferase
OS=Glycine max PE=1 SV=1 -0.91 -0.11 1.00 0.98 - 0.02 -0.10 0.90 1.00 0.02 0.08 1.0 0 0.97 -0.19 -0.87 0.93 0.98
T2DNF5
S-formylglutathione hydrolase
OS=Phaseolus vulgaris PE=2
SV=1
-0.27 -0.03 0.98 1.00 -0.33 -1. 06 0.99 0.96 -1.07 -2.25 1.00 0.46 -2.26 -3.41 0.98 0.12
S5RUY3
Sporulation related domain-
containing protein OS=Rhizobium
etli bv. mimosae str. Mim1
GN=REMIM1_CH01868 PE=4
SV=1
-0.14 -0.02 0.98 1.00 0.63 0.94 1.00 0.97 -0.43 -0.44 0.93 1.00 -0.32 -0.38 0.87 1.00
Q8VX07 Starch branching enzyme
(Fragment) OS=Phaseolus vulgaris 2.20 0.24 0.88 1.00 0.64 0.95 1.00 0.97 -0.39 -0.40 0.93 1.00 -0.30 -0.36 0.88 1.00

S28
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
GN=pvsbe2 PE=4 SV=1
T2DNN1
Steroid binding protein
OS=Phaseolus vulgaris PE=2
SV=1
-0.84 -0.10 1.00 0.99 -0.20 -1. 07 0.99 0.95 -0.51 -2.27 1.00 0.44 -0.92 -4.01 0.97 0.04
Q8GTA3 Sucrose synthase OS=Phaseolus
vulgaris PE=1 SV=1 1.07 0.11 0.99 1.00 -0.58 -0.74 0.94 1.00 -0.67 -0 .72 0.95 1.00 -0.58 -0.74 0.91 1.00
S5SWM9
Sugar ABC transporter substrate-
binding protein OS=Rhizobium etli
bv. mimosae str. Mim1
GN=REMIM1_PF00622 PE=4
SV=1
-1.90 -0.17 1.00 0.85 -0.03 -0. 03 0.92 1.00 -0.13 -0.13 0.96 1.00 -0.03 -0.03 0.95 0.95
C6SWE8 Superoxide dismutase [Cu-Zn]
OS=Glycine max PE=2 SV=1 -0.27 -0.03 0.98 1.00 0.0 7 0.37 1.00 0.96 -0.19 -0.82 0.97 1.00 -0.38 -1.78 1.00 0.68
A5JVZ7 Superoxide dismutase OS=Glycine
max PE=1 SV=1 -1.45 -0.15 1.0 0 0.89 -0.07 -0.19 0.89 1.00 -0.02 -0.04 0.98 0.99 -0.12 -0.35 0.88 1.00
I1LCI3 Superoxide dismutase OS=Glycine
max PE=3 SV=1 -1.27 -0.14 1.0 0 0.91 -0.18 -0.43 0.90 1.00 -0.41 -0.79 0.96 1.00 -0.28 -0.65 0.89 1.00
S5SL82
Tetratricopeptide repeat-containing
TonB-dependent receptor protein
OS=Rhizobium etli bv. mimosae
str. Mim1 GN=REMIM1_CH02733
PE=4 SV=1
-0.20 -0.02 0.98 1.00 0.00 0.00 0.93 1.00 -0.09 -0.09 0.97 1.00 0.00 0.00 0.97 0.95
I1J8A4 T hioredoxin reductase OS=Glycine
max PE=3 SV=2 -0.99 -0.10 1.0 0 0.99 -0.46 -0.68 0.94 1.00 -0.88 -0.99 0.98 0.99 -0.79 -1.04 0.95 0.94
S5RYF5
TonB-dependent
hemoglobin/transferrin/lactoferrin
family receptor protein
OS=Rhizobium etli bv. mimosae
str. Mim1 GN=REMIM1_CH03122
PE=4 SV=1
0.42 0.05 0.98 1.00 -0.34 -0.41 0.89 1.00 0.41 0.55 1.00 0.97 -0.34 -0.41 0.87 1.00
S3I214
Transcriptional regulator
OS=Rhizobium grahamii CCGE
502 GN=RGCCGE502_07104
PE=4 SV=1
0.51 0.05 0.98 1.00 0.02 0.02 0.93 1.00 -0.06 -0.06 0.98 0.99 0.02 0.02 0.98 0.94
C6T7V6 Triosephosphate isomerase
OS=Glycine max PE=1 SV=1 -0.18 -0.02 0.98 1.00 0.3 5 0.42 1.00 0.96 -0.08 -0.08 0.97 0.99 0.02 0.03 0.98 0.94
Q5JZZ3
Triosephosphate isomerase
OS=Phaseolus vulgaris var. nanus
GN=tpi PE=1 SV=1
0.10 0.01 0.98 1.00 -0.07 -0.64 0.93 1.00 -0.12 -0 .95 0.98 1.00 -0.25 -2.13 1.00 0.53
A2VBN4
Trypsin/chymotrypsin inhibitor
OS=Phaseolus vulgaris GN=BBI
PE=3 SV=1
0.90 0.08 0.99 1.00 0.08 0.74 1.00 0.96 0.22 1.94 0.48 0.99 -0.21 - 1.87 1.00 0.64
T2DLN1 Tubulin beta chain OS=Phaseolus
vulgaris PE=2 SV=1 0.51 0.05 0.98 1.00 -0.68 -2.16 1.00 0.42 -0.41 -1 .24 1.00 0.92 -0.81 -2.49 1.00 0.38
K7VK11
UDP-glucuronic acid
decarboxylase 1-like isoform 1
OS=Phaseolus vulgaris PE=2
SV=1
0.96 0.09 0.99 1.00 -0.42 -1.85 1.00 0.58 -0.25 -1 .03 0.99 0.98 -0.52 -2.19 1.00 0.50
K7LX62
Uncharacterized protein
(Fragment) OS=Glycine max PE=3
SV=1
-0.37 -0.04 0.98 1.00 0.00 0.00 0.93 1.00 0.40 0.45 1.00 0.97 0.34 0.41 1.00 0.91
K7KUJ1
Uncharacterized protein
(Fragment) OS=Glycine max PE=4
SV=1
0.44 0.04 0.98 1.00 -0.03 -0.10 0.90 1.00 -0.26 -0 .86 0.97 1.00 -0.27 -1.03 0.95 0.95

S29
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
K7L9E5
Uncharacterized protein
(Fragment) OS=Glycine max PE=4
SV=1
-2.40 -0.23 1.00 0.83 -0.35 -0. 42 0.90 1.00 -0.44 -0.46 0.93 1.00 -0.35 -0.42 0.88 1.00
I1KG91
Uncharacterized protein
(Fragment) OS=Glycine max PE=4
SV=1
-0.89 -0.09 1.00 1.00 0.00 0.00 0.93 1.00 -0.11 -0.11 0.96 1.00 0.00 0.00 0.96 0.95
K7KD30
Uncharacterized protein
(Fragment) OS=Glycine max PE=4
SV=1
1.96 0.20 0.93 1.00 0.04 0.04 0.94 1.00 -0.04 -0.04 0.99 0.99 0.04 0.04 0.99 0.94
K7MND3
Uncharacterized protein
(Fragment) OS=Glycine max PE=4
SV=1
-1.80 -0.19 1.00 0.83 0.31 0.68 1.00 0.96 0.14 0.26 1.00 0.97 0.00 0.00 0.97 0.95
C6TFP4 Uncharacterized protein
OS=Glycine max PE=1 SV=1 -0.05 -0.01 0.98 1.00 - 1.08 -1.50 1.00 0.76 -1.18 -1.36 1.00 0.87 -1.08 -1.50 1.00 0.80
C6TD56 Uncharacterized protein
OS=Glycine max PE=1 SV=1 0.72 0.08 0 .99 1.00 -0.39 -1.35 1.00 0.83 -0.14 -0.47 0.93 1.00 -0.49 -1.65 1.00 0.74
C6T073 Uncharacterized protein
OS=Glycine max PE=1 SV=1 1.29 0.13 0 .98 1.00 0.08 0.21 1.00 0.97 -0.10 -0.21 0.94 1.00 -0.64 -1.31 0.99 0.87
C6T172 Uncharacterized protein
OS=Glycine max PE=1 SV=1 -1.62 -0.17 1.00 0.85 - 0.02 -0.02 0.92 1.00 -0.11 -0.11 0.96 1.00 -0.02 -0.02 0.96 0.95
C6TMA8 Uncharacterized protein
OS=Glycine max PE=1 SV=1 -0.52 -0.05 0.98 1.00 0.0 1 0.01 0.93 1.00 -0.09 -0.08 0.97 0.99 0.01 0.01 0.97 0.94
C6SZN6 Uncharacterized protein
OS=Glycine max PE=1 SV=1 3.07 0.30 0 .84 1.00 0.12 0.25 1.00 0.97 -0.01 -0.02 0.99 0.98 0.12 0.25 1.00 0.92
C6SW33 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.26 -0.02 0.98 1.00 - 1.98 -3.01 1.00 0.11 -2.07 -2.53 1.00 0.33 -1.65 -2.86 1.00 0.26
C6T1V2 Uncharacterized protein
OS=Glycine max PE=2 SV=1 1.79 0.18 0 .96 1.00 -0.83 -1.49 1.00 0.76 -1.48 -1.75 1.00 0.70 -1.40 -2.02 1.00 0.58
C6TAB7 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -4.87 -0.54 1.00 0.03 - 1.01 -1.64 1.00 0.69 -0.94 -1.34 1.00 0.88 -1.34 -1.90 1.00 0.63
C6TGT0 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.58 0.05 0 .98 1.00 -0.23 -0.47 0.90 1.00 -0.92 -1.30 1.00 0.89 -0.55 -1.02 0.95 0.95
C6TGW2 Unchar acterized protein
OS=Glycine max PE=2 SV=1 -0.90 -0.09 1.00 1.00 - 1.09 -2.02 1.00 0.49 -0.69 -1.28 1.00 0.90 -1.09 -2.02 1.00 0.58
C6SZQ3 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.60 -0.19 1.00 0.84 - 0.96 -1.26 1.00 0.87 -1.07 -1.16 1.00 0.94 -0.96 -1.26 0.98 0.88
C6T8Y2 Uncharacterized protein
OS=Glycine max PE=2 SV=1 1.98 0.20 0 .94 1.00 -0.94 -1.26 1.00 0.87 -1.03 -1.16 1.00 0.95 -0.94 -1.26 0.98 0.89
C6SVV1 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.42 -0.15 1.00 0.88 - 0.89 -1.41 1.00 0.80 -0.81 -1.14 1.00 0.95 -1.22 -1.74 1.00 0.70
C6T827 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.40 -0.04 0.98 1.00 - 0.88 -1.39 1.00 0.81 -0.80 -1.10 0.99 0.96 -0.62 -1.07 0.96 0.94
C6TBY0 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.95 -0.20 1.00 0.83 - 0.80 -1.07 0.99 0.95 -0.90 -1.00 0.99 0.99 -0.80 -1.07 0.96 0.94
C6SXD0 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.30 0.03 0 .98 1.00 0.31 0.55 1.00 0.95 -0.88 -0.98 0.98 0.99 -0.22 -0.35 0.88 1.00
C6SXM4 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.26 -0.03 0.98 1.00 - 0.02 -0.10 0.90 1.00 -0.25 -0.96 0.98 1.00 -0.21 -0.92 0.94 0.97
C6T0H9 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.01 0.00 0 .98 1.00 -0.41 -1.19 1.00 0.90 -0.36 -0.93 0.98 1.00 -0.88 -2.19 1.00 0.50
C6TKH0 Uncharacterized protein
OS=Glycine max PE=2 SV=1 1.27 0.12 0 .99 1.00 -0.24 -1.05 0.99 0.96 -0.21 -0.81 0.96 1.00 -0.81 -2.91 1.00 0.25

S30
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
C6TCX5 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.56 -0.06 0.98 1.00 0.0 5 0.16 0.99 0.98 -0.27 -0.79 0.96 1.00 -0.04 -0.14 0.91 0.97
C6TLF4 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.33 0.03 0 .98 1.00 -0.59 -0.77 0.95 1.00 -0.67 -0.75 0.96 1.00 -0.27 -0.38 0.87 1.00
C6SZ88 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.38 0.04 0 .98 1.00 0.67 1.56 1.00 0.98 -0.48 -0.75 0.96 1.00 0.00 0.00 0.97 0.95
C6SWE6 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.64 -0.06 0.99 1.00 - 0.96 -1.30 1.00 0.85 -0.55 -0.74 0.96 1.00 -0.63 -0.94 0.94 0.97
C6SZW2 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.16 -0.01 0.98 1.00 - 0.32 -0.39 0.89 1.00 -0.41 -0.43 0.93 1.00 -0.32 -0.39 0.87 1.00
C6T2E5 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.09 -0.12 1.00 0.95 - 0.14 -0.28 0.89 1.00 -0.25 -0.43 0.93 1.00 -0.45 -0.83 0.92 0.99
C6T1L4 Uncharacterized protein
OS=Glycine max PE=2 SV=1 2.15 0.22 0 .91 1.00 -0.05 -0.18 0.89 1.00 -0.13 -0.39 0.93 1.00 -0.23 -0.76 0.91 0.99
C6SWE0 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.76 0.09 0 .99 1.00 0.01 0.02 0.93 1.00 -0.14 -0.35 0.93 1.00 -0.55 -1.32 0.99 0.86
C6SV94 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.73 -0.08 0.99 1.00 0.1 3 0.66 1.00 0.96 -0.07 -0.30 0.93 1.00 0.06 0.30 1.00 0.91
C6T977 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.47 0.05 0 .98 1.00 0.01 0.02 0.93 1.00 -0.17 -0.22 0.94 1.00 0.01 0.02 0.98 0.94
C6TJH2 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.14 -0.01 0.98 1.00 - 0.38 -0.85 0.96 1.00 -0.10 -0.21 0.94 1.00 -1.58 -2.33 1.00 0.44
C6SVD7 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.62 -0.07 0.99 1.00 - 0.01 -0.01 0.92 1.00 -0.11 -0.11 0.96 1.00 -0.01 -0.01 0.96 0.95
C6T588 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.25 -0.13 1.00 0.94 - 0.02 -0.02 0.92 1.00 -0.11 -0.11 0.96 1.00 -0.02 -0.02 0.96 0.95
C6TGD9 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -1.63 -0.18 1.00 0.85 - 0.02 -0.02 0.92 1.00 -0.11 -0.11 0.96 1.00 -0.02 -0.02 0.95 0.95
C6SX86 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.48 0.05 0 .98 1.00 -0.02 -0.03 0.92 1.00 -0.11 -0.11 0.96 1.00 -0.02 -0.03 0.95 0.95
C6SYC1 Uncharacterized protein
OS=Glycine max PE=2 SV=1 1.15 0.11 0 .99 1.00 0.01 0.01 0.93 1.00 -0.09 -0.09 0.97 0.99 0.01 0.01 0.97 0.94
C6SW24 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.18 -0.02 0.98 1.00 0.3 3 0.41 1.00 0.96 -0.09 -0.09 0.97 0.99 0.57 0.75 1.00 0.92
C6T9Z5 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.65 -0.07 0.99 1.00 - 0.22 -0.58 0.92 1.00 0.01 0.03 1.0 0 0.98 -0.60 -1.40 1.00 0.84
C6SXB4 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.80 0.08 0 .99 1.00 -0.93 -1.26 1.00 0.87 0.05 0.07 1.00 0.97 -0.61 -0.93 0.94 0.97
C6TCC6 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.32 0.03 0 .98 1.00 -0.34 -0.42 0.90 1.00 0.06 0.08 1.00 0.97 -0.34 -0.42 0.88 1.00
C6SW04 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.46 0.04 0 .98 1.00 0.08 0.18 1.00 0.98 0.09 0.21 1.00 0.97 0.08 0.18 1.00 0.92
C6T389 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.63 0.06 0 .99 1.00 0.54 1.01 1.00 0.97 0.21 0.33 1.00 0.97 0.00 0.00 0.97 0.95
C6SWV3 Uncharacterized protein
OS=Glycine max PE=2 SV=1 0.08 0.01 0 .98 1.00 0.00 0.00 0.93 1.00 0.10 0.34 1.00 0.97 -0.31 -1.02 0.95 0.95
C6SWG9 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.07 -0.01 0.98 1.00 0.0 1 0.02 0.93 1.00 0.41 0.62 1.00 0.98 -0.57 -0.72 0.91 1.00
C6T4R9 Uncharacterized protein
OS=Glycine max PE=2 SV=1 -0.21 -0.02 0.98 1.00 0.7 1 3.47 0.01 0.98 0.29 1.14 1.00 0.99 0.15 0.66 1.00 0.92

S31
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
I1MC31 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -2.03 -0.20 1.00 0.83 - 0.72 -3.29 0.99 0.06 -1.44 -4.28 0.99 0.00 -1.13 -4.49 0.96 0.01
I1KPN3 Unch aracterized protein
OS=Glycine max PE=3 SV=1 -1.26 -0.13 1.00 0.92 - 0.42 -3.21 0.99 0.07 -0.42 -2.81 1.00 0.21 -0.57 -4.17 0.97 0.03
I1MJU7 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -0.88 -0.09 1.00 1.00 - 0.34 -1.29 1.00 0.85 -1.00 -2.61 1.00 0.30 -0.80 -2.60 1.00 0.34
I1L655 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -1.45 -0.15 1.00 0.89 - 1.00 -2.78 1.00 0.17 -1.07 -2.48 1.00 0.35 -1.09 -2.91 1.00 0.25
I1KP71 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -0.31 -0.03 0.98 1.00 - 0.12 -0.99 0.98 0.98 -0.35 -2.33 1.00 0.42 -0.28 -2.19 1.00 0.50
I1MDT4 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.29 0.03 0 .98 1.00 -0.25 -1.40 1.00 0.80 -0.49 -2.26 1.00 0.45 -0.42 -2.24 1.00 0.48
I1LFG6 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.00 0.00 0 .98 1.00 -1.68 -2.49 1.00 0.27 -1.76 -2.11 1.00 0.52 -1.68 -2.49 1.00 0.38
I1JT28 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -2.57 -0.26 1.00 0.85 - 0.06 -0.17 0.89 1.00 -0.90 -1.68 1.00 0.73 -0.94 -2.00 1.00 0.58
I1L314 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -0.43 -0.04 0.98 1.00 - 1.32 -1.86 1.00 0.58 -1.40 -1.63 1.00 0.76 -1.32 -1.86 1.00 0.65
I1JVE0 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.84 0.09 0 .99 1.00 0.34 0.80 1.00 0.96 -1.41 -1.61 1.00 0.76 0.00 0.01 0.97 0.94
I1JGP8 Uncharacterized protein
OS=Glycine max PE=3 SV=1 2.00 0.18 0 .96 1.00 -1.20 -1.70 1.00 0.66 -1.30 -1.50 1.00 0.81 -1.20 -1.70 1.00 0.71
I1N7G4 Unch aracterized protein
OS=Glycine max PE=3 SV=1 0.62 0.06 0 .98 1.00 -0.54 -1.24 1.00 0.88 -0.78 -1.43 1.00 0.84 -0.67 -1.47 1.00 0.81
I1KAB7 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -1.24 -0.12 1.00 0.95 - 0.05 -0.26 0.89 1.00 -0.31 -1.43 1.00 0.84 -0.06 -0.32 0.88 0.99
I1KDL4 Uncharacterized protein
OS=Glycine max PE=3 SV=1 1.01 0.11 0 .99 1.00 -0.45 -1.15 1.00 0.91 -0.69 -1.40 1.00 0.86 -0.45 -1.15 0.97 0.92
I1M5L7 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -0.77 -0.08 1.00 1.00 - 0.31 -0.57 0.92 1.00 -1.19 -1.35 1.00 0.88 -1.10 -1.53 1.00 0.79
I1JJB5 Uncharacterized protein
OS=Glycine max PE=3 SV=1 1.67 0.17 0 .96 1.00 -0.50 -1.22 1.00 0.88 -0.64 -1.32 1.00 0.89 -1.49 -2.55 1.00 0.36
I1KAJ5 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.38 0.04 0 .98 1.00 -0.46 -1.34 1.00 0.83 -0.51 -1.30 1.00 0.90 -0.14 -0.46 0.88 1.00
I1M3M0 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -0.52 -0.07 0.99 1.00 - 0.57 -1.14 1.00 0.92 -0.78 -1.26 1.00 0.91 -0.93 -1.65 1.00 0.74
I1KQ93 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -1.95 -0.22 1.00 0.82 - 0.11 -0.41 0.89 1.00 -0.42 -1.23 1.00 0.92 -0.16 -0.56 0.88 1.00
I1K135 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.38 0.04 0 .98 1.00 -0.59 -1.71 1.00 0.65 -0.46 -1.18 1.00 0.94 -0.67 -1.89 1.00 0.63
I1KPA1 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.73 0.08 0 .99 1.00 -0.16 -0.27 0.89 1.00 -1.03 -1.16 1.00 0.94 -0.93 -1.27 0.98 0.88
I1KZJ0 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.81 0.08 0 .99 1.00 -0.94 -1.27 1.00 0.86 -1.03 -1.16 1.00 0.94 -0.94 -1.27 0.99 0.88
I1JDH6 Uncharacterized protein
OS=Glycine max PE=3 SV=1 1.10 0.11 0 .99 1.00 0.01 0.02 0.93 1.00 -0.60 -1.09 0.99 0.96 -0.17 -0.40 0.87 1.00
I1MUR2 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -2.06 -0.21 1.00 0.82 - 0.79 -1.05 0.99 0.96 -0.88 -0.98 0.98 1.00 -0.79 -1.05 0.96 0.94
I1LCI1 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -1.10 -0.10 1.00 0.98 - 0.74 -1.28 1.00 0.86 -0.60 -0.95 0.98 1.00 -0.74 -1.28 0.99 0.88

S32
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
I1KMP3 Uncharacterized protein
OS=Glycine max PE=3 SV=1 2.40 0.22 0 .92 1.00 -0.76 -1.00 0.99 0.98 -0.84 -0.92 0.98 1.00 -0.76 -1.00 0.95 0.95
I1JJL6 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -0.33 -0.03 0.98 1.00 - 0.12 -0.55 0.91 1.00 -0.20 -0.78 0.96 1.00 -0.38 -1.61 1.00 0.75
I1MA56 Unch aracterized protein
OS=Glycine max PE=3 SV=1 -0.56 -0.06 0.98 1.00 - 0.07 -0.36 0.89 1.00 -0.16 -0.77 0.96 1.00 -0.07 -0.36 0.88 1.00
I1JXH8 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -1.33 -0.14 1.00 0.92 - 0.59 -0.76 0.95 1.00 -0.69 -0.74 0.96 1.00 -0.59 -0.76 0.91 1.00
K7MVD3 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.99 0.10 0 .99 1.00 -0.23 -1.41 1.00 0.80 -0.13 -0.72 0.95 1.00 -0.51 -2.92 1.00 0.24
I1JYK1 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.57 0.05 0 .98 1.00 -0.57 -0.73 0.94 1.00 -0.66 -0.72 0.95 1.00 -0.57 -0.73 0.91 1.00
I1MTR0 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -0.36 -0.04 0.98 1.00 - 0.57 -0.72 0.94 1.00 -0.66 -0.71 0.95 1.00 -0.57 -0.72 0.91 1.00
I1KTW3 Uncharacterized protein
OS=Glycine max PE=3 SV=1 1.23 0.12 0 .99 1.00 -0.57 -0.72 0.94 1.00 -0.66 -0.70 0.95 1.00 -0.57 -0.72 0.91 1.00
I1J4X9 Unch aracterized protein
OS=Glycine max PE=3 SV=1 -0.02 0.00 0.98 1.00 - 0.34 -0.99 0.98 0.98 -0.26 -0.69 0.95 1.00 -0.58 -1.56 1.00 0.77
I1MT10 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.28 0.03 0 .98 1.00 -0.04 -0.40 0.89 1.00 -0.07 -0.66 0.95 1.00 -0.33 -3.21 0.99 0.17
K7LZH1 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -2.15 -0.21 1.00 0.82 - 0.07 -0.43 0.90 1.00 -0.12 -0.60 0.94 1.00 -0.24 -1.35 0.99 0.85
I1NF03 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -0.41 -0.04 0.98 1.00 - 0.02 -0.03 0.92 1.00 -0.39 -0.51 0.93 1.00 -0.79 -1.04 0.95 0.94
I1KU96 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.65 0.07 0 .99 1.00 0.17 0.30 1.00 0.96 -0.38 -0.50 0.93 1.00 -0.19 -0.31 0.88 0.99
I1KAY1 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -2.26 -0.26 1.00 0.85 - 0.34 -0.41 0.89 1.00 -0.41 -0.44 0.93 1.00 -0.34 -0.41 0.87 1.00
I1NAI7 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.07 0.01 0 .98 1.00 0.05 0.36 1.00 0.96 -0.05 -0.30 0.93 1.00 -0.13 -0.89 0.93 0.98
I1KIP9 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -1.47 -0.15 1.00 0.89 0.3 1 0.39 1.00 0.96 -0.11 -0.12 0.96 1.00 -0.02 -0.03 0.95 0.95
I1NJ85 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -1.49 -0.15 1.00 0.89 0.0 3 0.18 1.00 0.98 -0.02 -0.11 0.96 1.00 -0.03 -0.18 0.90 0.97
I1J5A5 Unch aracterized protein
OS=Glycine max PE=3 SV=1 0.15 0.01 0 .98 1.00 -0.02 -0.02 0.92 1.00 -0.11 -0.11 0.96 1.00 -0.02 -0.02 0.95 0.95
I1JRF2 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.47 0.05 0 .98 1.00 0.00 0.00 0.93 1.00 -0.10 -0.10 0.97 1.00 0.00 0.00 0.97 0.95
I1KJN4 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.22 0.02 0 .98 1.00 -0.01 -0.01 0.93 1.00 -0.10 -0.09 0.97 0.99 -0.01 -0.01 0.96 0.95
I1L1Q8 Uncharacterized protein
OS=Glycine max PE=3 SV=1 1.21 0.12 0 .99 1.00 0.00 0.00 0.93 1.00 -0.09 -0.09 0.97 0.99 0.00 0.00 0.97 0.95
K7L2A0 Uncharacterized protein
OS=Glycine max PE=3 SV=1 1.62 0.18 0 .96 1.00 -0.01 -0.01 0.93 1.00 -0.09 -0.09 0.97 0.99 -0.01 -0.01 0.96 0.95
I1LNE6 Uncharacterized protein
OS=Glycine max PE=3 SV=1 1.01 0.10 0 .99 1.00 0.00 0.00 0.93 1.00 -0.08 -0.09 0.97 0.99 0.00 0.00 0.97 0.95
I1JMB4 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.35 0.04 0 .98 1.00 -0.01 -0.01 0.92 1.00 -0.08 -0.08 0.97 0.99 -0.01 -0.01 0.96 0.95
I1JQX3 Uncharacterized protein
OS=Glycine max PE=3 SV=1 1.42 0.14 0 .98 1.00 0.02 0.03 0.94 1.00 -0.08 -0.08 0.97 0.99 0.02 0.03 0.98 0.94

S33
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
I1KG78 Uncharacterized protein
OS=Glycine max PE=3 SV=1 1.18 0.11 0 .99 1.00 0.02 0.03 0.94 1.00 -0.08 -0.07 0.97 0.99 0.02 0.03 0.98 0.94
I1JY29 Unch aracterized protein
OS=Glycine max PE=3 SV=1 1.86 0.19 0 .95 1.00 0.03 0.03 0.94 1.00 -0.07 -0.07 0.98 0.99 0.03 0.03 0.98 0.94
K7KAJ1 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.07 0.01 0 .98 1.00 0.00 0.00 0.93 1.00 -0.04 -0.06 0.98 0.99 -0.63 -1.11 0.96 0.93
K7KWB0 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -0.08 -0.01 0.98 1.00 0.0 2 0.03 0.94 1.00 -0.03 -0.04 0.98 0.99 0.02 0.03 0.98 0.94
I1LIN0 Uncharacterized protein
OS=Glycine max PE=3 SV=1 2.53 0.23 0 .90 1.00 0.05 0.06 0.95 1.00 -0.04 -0.04 0.98 0.99 0.05 0.06 1.00 0.94
I1JPW5 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -1.37 -0.14 1.00 0.90 0.1 4 1.10 1.00 0.97 0.04 0.26 1.00 0.97 -0.15 -1.11 0.96 0.93
I1JD78 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.32 0.04 0 .98 1.00 -0.29 -0.66 0.93 1.00 0.12 0.28 1.00 0.97 -0.52 -1.11 0.96 0.93
I1MAE6 Uncharacterized protein
OS=Glycine max PE=3 SV=1 1.29 0.14 0 .98 1.00 -0.27 -0.72 0.94 1.00 0.14 0.37 1.00 0.97 -0.27 -0.72 0.91 1.00
I1JW22 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -1.13 -0.11 1.00 0.97 0.7 7 1.01 1.00 0.97 0.40 0.45 1.00 0.97 -0.01 -0.01 0.96 0.95
I1KGY6 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -0.43 -0.04 0.98 1.00 0.0 0 -0.01 0.93 1.00 0.40 0.51 1.00 0.97 - 0.33 -0.39 0.87 1.00
K7LDQ4 Uncharacterized protein
OS=Glycine max PE=3 SV=1 0.07 0.01 0 .98 1.00 0.25 0.35 1.00 0.96 0.41 0.54 1.00 0.97 -0.34 -0.40 0.87 1.00
I1JGR5 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -1.93 -0.20 1.00 0.83 0.0 6 0.60 1.00 0.96 0.07 0.67 1.00 0.98 -0.06 -0.61 0.89 1.00
I1MB04 Unch aracterized protein
OS=Glycine max PE=3 SV=1 0.99 0.11 0 .99 1.00 0.01 0.01 0.93 1.00 0.60 0.95 1.00 0.98 0.01 0.01 0.97 0.94
I1JCI6 Uncharacterized protein
OS=Glycine max PE=3 SV=1 1.04 0.10 0 .99 1.00 -0.01 -0.01 0.93 1.00 0.60 0.96 1.00 0.98 -0.25 -0.35 0.88 1.00
I1KRC0 Uncharacterized protein
OS=Glycine max PE=3 SV=1 -2.38 -0.22 1.00 0.82 1.1 0 2.07 0.44 0.99 0.91 1.55 0.80 0.99 0.83 1.50 1.00 0.95
I1LF29 Unch aracterized protein
OS=Glycine max PE=3 SV=1 -2.29 -0.22 1.00 0.82 0.0 0 0.00 0.93 1.00 1.19 1.59 0.78 0.99 0.00 0.00 0.97 0.95
I1L4U3 Uncharacterized protein
OS=Glycine max PE=3 SV=2 -0.43 -0.04 0.98 1.00 - 0.44 -1.29 1.00 0.86 -0.70 -1.67 1.00 0.74 -0.76 -2.04 1.00 0.57
I1MW49 Uncharacterized protein
OS=Glycine max PE=3 SV=2 -0.92 -0.09 1.00 1.00 - 0.34 -1.64 1.00 0.69 -0.29 -1.25 1.00 0.91 -0.50 -2.27 1.00 0.47
I1MJE1 Uncharacterized protein
OS=Glycine max PE=3 SV=2 0.58 0.06 0 .99 1.00 -0.27 -0.63 0.93 1.00 -0.41 -0.82 0.96 1.00 -0.27 -0.63 0.89 1.00
I1NIE7 Uncharacterized protein
OS=Glycine max PE=3 SV=2 1.20 0.13 0 .99 1.00 -0.20 -0.31 0.89 1.00 -0.37 -0.47 0.93 1.00 -0.78 -0.99 0.95 0.96
I1KD00 Uncharacterized protein
OS=Glycine max PE=3 SV=2 -0.53 -0.06 0.99 1.00 - 0.02 -0.03 0.92 1.00 -0.12 -0.12 0.96 1.00 -0.02 -0.03 0.95 0.95
I1L0N6 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.74 -0.16 1.00 0.87 - 0.16 -1.61 1.00 0.71 -0.40 -3.29 1.00 0.06 -0.27 -2.69 1.00 0.31
I1JXA0 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.45 0.04 0 .98 1.00 -0.06 -0.24 0.89 1.00 -1.06 -2.71 1.00 0.25 -0.79 -2.65 1.00 0.33
K7LQ69 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.91 0.08 0 .99 1.00 -0.42 -2.60 1.00 0.23 -0.48 -2.51 1.00 0.34 -0.48 -2.92 1.00 0.24
K7KWZ7 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.74 0.07 0 .99 1.00 -0.56 -1.97 1.00 0.52 -0.88 -2.38 1.00 0.40 -0.52 -1.84 1.00 0.65

S34
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
I1LH18 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.57 -0.16 1.00 0.87 - 0.31 -0.87 0.97 1.00 -1.26 -2.18 1.00 0.49 -0.67 -1.67 1.00 0.73
C6SWX1 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -2.36 -0.23 1.00 0.83 - 0.83 -1.62 1.00 0.70 -1.69 -2.03 1.00 0.57 -1.03 -1.88 1.00 0.64
I1LT76 Unch aracterized protein
OS=Glycine max PE=4 SV=1 -0.43 -0.04 0.98 1.00 - 0.67 -1.97 1.00 0.52 -0.84 -2.02 1.00 0.57 -1.02 -2.65 1.00 0.33
I1KU21 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.59 -0.16 1.00 0.87 - 0.17 -1.28 1.00 0.86 -0.31 -1.97 1.00 0.60 -0.48 -3.36 0.99 0.14
I1LU53 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.38 -0.13 1.00 0.92 - 1.21 -2.49 1.00 0.27 -1.01 -1.91 1.00 0.62 -1.66 -2.86 1.00 0.26
I1N8M5 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.13 -0.12 1.00 0.94 - 0.47 -1.34 1.00 0.83 -0.79 -1.77 1.00 0.69 -1.81 -3.18 0.99 0.18
K7LWE4 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.19 0.12 0 .99 1.00 -0.51 -1.63 1.00 0.70 -0.65 -1.75 1.00 0.70 -1.05 -2.80 1.00 0.28
I1KFR0 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.32 0.12 0 .99 1.00 -0.28 -0.64 0.93 1.00 -1.19 -1.73 1.00 0.71 -0.65 -1.33 0.99 0.86
I1L849 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.09 0.11 0 .99 1.00 -0.07 -0.47 0.90 1.00 -0.27 -1.66 1.00 0.74 -0.42 -2.73 1.00 0.30
I1LCN7 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.63 -0.06 0.98 1.00 - 0.74 -1.31 1.00 0.84 -1.42 -1.64 1.00 0.75 -1.31 -1.87 1.00 0.64
I1LM73 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.55 0.06 0 .98 1.00 -0.26 -0.86 0.96 1.00 -0.62 -1.64 1.00 0.75 -1.03 -2.71 1.00 0.31
I1J8K6 Unch aracterized protein
OS=Glycine max PE=4 SV=1 0.79 0.08 0 .99 1.00 -1.32 -1.88 1.00 0.57 -1.41 -1.63 1.00 0.76 -1.32 -1.88 1.00 0.64
I1LVR2 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.24 0.02 0 .98 1.00 -0.74 -1.27 1.00 0.86 -1.40 -1.62 1.00 0.76 -0.74 -1.27 0.99 0.88
I1LH56 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.12 -0.12 1.00 0.95 - 0.21 -0.66 0.93 1.00 -0.65 -1.56 1.00 0.79 -0.40 -1.19 0.98 0.90
I1MRX8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.64 0.15 0 .98 1.00 -0.73 -1.61 1.00 0.71 -0.83 -1.55 1.00 0.79 -0.86 -1.83 1.00 0.66
K7L7D2 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.75 -0.17 1.00 0.86 - 0.15 -0.61 0.92 1.00 -0.46 -1.45 1.00 0.83 -0.62 -2.10 1.00 0.54
K7MIV6 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.86 -0.18 1.00 0.84 - 0.66 -1.57 1.00 0.72 -0.70 -1.44 1.00 0.84 -0.66 -1.57 1.00 0.77
I1MKY2 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.07 0.12 0 .99 1.00 -1.07 -1.48 1.00 0.76 -1.15 -1.35 1.00 0.88 -1.07 -1.48 1.00 0.80
K7MIH4 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.45 -0.16 1.00 0.88 - 0.24 -0.50 0.90 1.00 -0.92 -1.34 1.00 0.88 -1.33 -1.93 1.00 0.62
I1J6Z7 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.37 -0.04 0.98 1.00 - 1.10 -1.51 1.00 0.75 -1.17 -1.34 1.00 0.88 -1.10 -1.51 1.00 0.79
I1LEE5 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.90 -0.20 1.00 0.83 - 1.19 -1.91 1.00 0.55 -0.80 -1.27 1.00 0.91 -1.19 -1.91 1.00 0.62
I1MD61 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.54 0.06 0 .98 1.00 -0.29 -1.24 1.00 0.87 -0.32 -1.22 1.00 0.92 -0.54 -2.19 1.00 0.50
I1K8E3 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.46 -0.05 0.98 1.00 - 0.94 -1.28 1.00 0.86 -1.03 -1.17 1.00 0.94 -0.94 -1.28 0.99 0.88
I1KZJ9 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.20 -0.02 0.98 1.00 - 0.16 -0.28 0.89 1.00 -1.03 -1.15 1.00 0.95 -0.93 -1.25 0.98 0.89
I1JLC8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.64 0.08 0 .99 1.00 0.01 0.04 0.94 1.00 -0.17 -1.14 1.00 0.95 -0.20 -1.51 1.00 0.79

S35
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
I1MQS6 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.01 0.10 0 .99 1.00 -0.73 -1.73 1.00 0.65 -0.45 -1.02 0.99 0.99 -0.73 -1.73 1.00 0.70
I1LJP3 Unch aracterized protein
OS=Glycine max PE=4 SV=1 -0.07 -0.01 0.98 1.00 - 0.13 -0.35 0.89 1.00 -0.44 -1.01 0.99 0.99 -0.26 -0.72 0.91 1.00
K7LY40 Unchar acterized protein
OS=Glycine max PE=4 SV=1 -2.13 -0.22 1.00 0.82 - 0.51 -1.33 1.00 0.84 -0.44 -1.00 0.99 0.99 -0.61 -1.52 1.00 0.79
K7KYL8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.44 0.04 0 .98 1.00 -0.14 -0.37 0.89 1.00 -0.46 -1.00 0.99 0.99 -0.39 -0.97 0.94 0.96
I1LGX3 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.97 -0.18 1.00 0.84 0.3 0 0.54 1.00 0.95 -0.88 -0.97 0.98 1.00 -0.46 -0.67 0.90 1.00
I1JTY8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.20 0.02 0 .98 1.00 -0.09 -0.29 0.89 1.00 -0.36 -0.95 0.98 1.00 -0.37 -1.12 0.97 0.92
I1L957 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.81 0.09 0 .99 1.00 -0.77 -1.01 0.99 0.97 -0.86 -0.95 0.98 1.00 -0.77 -1.01 0.95 0.95
K7KSG7 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.86 0.08 0 .99 1.00 -0.78 -1.03 0.99 0.97 -0.87 -0.95 0.98 1.00 -0.78 -1.03 0.95 0.95
I1JXS8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -2.09 -0.23 1.00 0.83 - 0.19 -0.77 0.95 1.00 -0.27 -0.93 0.98 1.00 -0.16 -0.63 0.89 1.00
I1M561 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.67 -0.07 0.99 1.00 - 0.28 -0.76 0.95 1.00 -0.38 -0.87 0.97 1.00 -0.45 -1.15 0.97 0.92
I1LVC1 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.26 -0.02 0.98 1.00 0.3 4 0.91 1.00 0.97 -0.42 -0.82 0.96 1.00 0.34 0.91 1.00 0.93
I1N2Z5 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.18 -0.11 1.00 0.97 - 0.05 -0.21 0.89 1.00 -0.22 -0.81 0.96 1.00 -0.16 -0.68 0.90 1.00
K7MMG8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -2.43 -0.25 1.00 0.84 - 0.27 -0.39 0.89 1.00 -0.69 -0.76 0.96 1.00 -0.27 -0.39 0.87 1.00
I1LDX0 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.48 0.05 0 .98 1.00 0.00 0.00 0.93 1.00 -0.48 -0.76 0.96 1.00 -0.26 -0.50 0.88 1.00
K7LM33 Uncharact erized protein
OS=Glycine max PE=4 SV=1 -1.03 -0.10 1.00 0.99 - 0.60 -0.77 0.95 1.00 -0.69 -0.75 0.96 1.00 -0.60 -0.77 0.91 0.99
K7KM59 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.11 -0.01 0.98 1.00 0.1 1 0.24 1.00 0.97 -0.47 -0.74 0.95 1.00 -0.12 -0.24 0.89 0.98
I1M4W1 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.46 -0.04 0.98 1.00 - 0.58 -0.75 0.95 1.00 -0.68 -0.73 0.95 1.00 -0.58 -0.75 0.91 1.00
I1LQS4 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.62 -0.07 0.99 1.00 - 0.60 -0.76 0.95 1.00 -0.68 -0.73 0.95 1.00 -0.26 -0.37 0.88 1.00
C6T250 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.25 0.02 0 .98 1.00 -0.58 -0.74 0.95 1.00 -0.66 -0.73 0.95 1.00 -0.24 -0.34 0.88 1.00
K7LDT9 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.69 -0.07 0.99 1.00 - 0.59 -0.74 0.94 1.00 -0.68 -0.72 0.95 1.00 0.19 0.30 1.00 0.91
I1J7C7 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.76 0.07 0 .99 1.00 0.14 0.26 1.00 0.97 -0.53 -0.72 0.95 1.00 -0.61 -0.92 0.94 0.97
C6SZ18 Uncharacterized protein
OS=Glycine max PE=4 SV=1 3.09 0.34 0 .88 1.00 -0.92 -1.25 1.00 0.87 -0.52 -0.71 0.95 1.00 -0.92 -1.25 0.98 0.89
I1JR88 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.00 0.10 0 .99 1.00 -0.57 -0.72 0.94 1.00 -0.66 -0.71 0.95 1.00 -0.57 -0.72 0.90 1.00
I1LAA7 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.20 0.02 0 .98 1.00 -0.56 -0.70 0.94 1.00 -0.67 -0.70 0.95 1.00 -0.56 -0.70 0.90 1.00
I1KXC2 Unch aracterized protein
OS=Glycine max PE=4 SV=1 0.09 0.01 0 .98 1.00 0.16 0.96 1.00 0.97 -0.14 -0.66 0.95 1.00 -0.11 -0.63 0.89 1.00

S36
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
I1NEH6 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.78 -0.08 1.00 1.00 - 0.30 -0.68 0.93 1.00 -0.33 -0.65 0.95 1.00 -0.73 -1.44 1.00 0.82
I1J8H1 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.17 -0.02 0.98 1.00 - 0.06 -0.29 0.89 1.00 -0.13 -0.61 0.94 1.00 -0.18 -0.89 0.93 0.98
I1L0Q8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.68 -0.16 1.00 0.88 - 0.33 -1.07 0.99 0.95 -0.18 -0.54 0.93 1.00 -1.93 -3.37 0.98 0.13
I1MDY5 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -2.00 -0.19 1.00 0.83 0.0 9 0.30 1.00 0.96 -0.19 -0.53 0.93 1.00 -0.97 -2.41 1.00 0.41
K7N193 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.57 -0.06 0.99 1.00 0.2 1 0.56 1.00 0.95 -0.25 -0.53 0.93 1.00 -0.18 -0.42 0.88 1.00
I1L8R1 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.32 0.03 0 .98 1.00 -0.51 -1.31 1.00 0.85 -0.21 -0.51 0.93 1.00 -0.61 -1.50 1.00 0.80
I1MC13 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.98 0.10 0 .99 1.00 0.17 0.30 1.00 0.96 -0.37 -0.49 0.93 1.00 -0.44 -0.65 0.90 1.00
I1KNH4 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.83 -0.19 1.00 0.83 - 0.34 -0.42 0.90 1.00 -0.45 -0.47 0.93 1.00 -0.34 -0.42 0.88 1.00
I1K8F4 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.92 -0.19 1.00 0.84 - 0.34 -0.41 0.89 1.00 -0.45 -0.47 0.93 1.00 -0.34 -0.41 0.88 1.00
C6SXC3 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.07 -0.01 0.98 1.00 - 0.35 -0.44 0.90 1.00 -0.43 -0.46 0.93 1.00 -0.35 -0.44 0.88 1.00
K7MRI3 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.11 0.01 0 .98 1.00 -0.35 -0.43 0.90 1.00 -0.44 -0.46 0.93 1.00 -0.35 -0.43 0.88 1.00
I1L2M6 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.12 -0.01 0.98 1.00 0.2 4 0.34 1.00 0.96 -0.43 -0.45 0.93 1.00 -0.34 -0.41 0.87 1.00
I1K7F4 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.52 -0.05 0.98 1.00 0.4 3 0.63 1.00 0.96 -0.44 -0.45 0.93 1.00 -0.34 -0.41 0.87 1.00
I1M0B5 Unch aracterized protein
OS=Glycine max PE=4 SV=1 -0.81 -0.08 1.00 1.00 - 0.34 -0.41 0.89 1.00 -0.43 -0.44 0.93 1.00 -0.34 -0.41 0.88 1.00
I1KZT2 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.56 0.06 0 .99 1.00 -0.10 -0.22 0.89 1.00 -0.23 -0.44 0.93 1.00 -0.47 -0.94 0.94 0.97
I1KM24 Unch aracterized protein
OS=Glycine max PE=4 SV=1 1.28 0.14 0 .98 1.00 -0.35 -0.42 0.89 1.00 -0.43 -0.44 0.93 1.00 -0.35 -0.42 0.88 1.00
I1LFG4 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.06 0.00 0.98 1.00 0.0 1 0.01 0.93 1.00 -0.42 -0.44 0.93 1.00 -0.33 -0.40 0.87 1.00
I1JNC7 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.05 0.00 0.98 1.00 - 0.32 -0.39 0.89 1.00 -0.41 -0.43 0.93 1.00 -0.32 -0.39 0.87 1.00
K7K978 Unchar acterized protein
OS=Glycine max PE=4 SV=1 -0.26 -0.03 0.98 1.00 0.0 1 0.02 0.93 1.00 -0.42 -0.43 0.93 1.00 -0.33 -0.39 0.87 1.00
I1KJ84 Unch aracterized protein
OS=Glycine max PE=4 SV=1 -1.36 -0.15 1.00 0.89 0.1 3 0.24 1.00 0.97 -0.23 -0.36 0.93 1.00 -0.63 -0.96 0.94 0.96
I1JPI7 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.18 0.02 0 .98 1.00 0.25 0.48 1.00 0.95 -0.22 -0.33 0.93 1.00 -0.96 -1.30 0.99 0.87
C6T2W8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.61 -0.06 0.98 1.00 - 0.95 -1.27 1.00 0.86 -0.22 -0.33 0.93 1.00 -0.95 -1.27 0.99 0.88
I1LQL0 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.60 0.17 0 .96 1.00 -0.08 -0.34 0.89 1.00 -0.06 -0.24 0.94 1.00 -0.08 -0.34 0.88 1.00
I1J8M6 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.08 -0.11 1.00 0.97 - 0.01 -0.02 0.92 1.00 -0.08 -0.16 0.95 1.00 -0.49 -0.98 0.95 0.96
I1KH65 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.04 0.00 0.98 1.00 0.5 6 0.72 1.00 0.96 -0.13 -0.12 0.96 1.00 -0.02 -0.02 0.96 0.95

S37
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
I1KDV2 Unch aracterized protein
OS=Glycine max PE=4 SV=1 -2.17 -0.25 1.00 0.84 - 0.03 -0.03 0.92 1.00 -0.11 -0.11 0.96 1.00 -0.03 -0.03 0.95 0.95
K7MZ08 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.78 0.08 0 .99 1.00 -0.02 -0.02 0.92 1.00 -0.10 -0.10 0.97 1.00 -0.02 -0.02 0.96 0.95
K7LID1 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.58 -0.05 0.98 1.00 0.7 7 1.01 1.00 0.97 -0.10 -0.10 0.97 1.00 0.01 0.01 0.97 0.94
I1K547 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.59 -0.06 0.99 1.00 - 0.01 -0.01 0.93 1.00 -0.10 -0.10 0.97 1.00 -0.01 -0.01 0.96 0.95
I1N3A6 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.89 0.09 0 .99 1.00 0.00 0.00 0.93 1.00 -0.10 -0.10 0.97 1.00 0.00 0.00 0.97 0.95
I1MMH5 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.07 0.01 0 .98 1.00 0.00 0.00 0.93 1.00 -0.10 -0.10 0.97 1.00 0.00 0.00 0.97 0.95
I1NBD9 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.08 0.01 0 .98 1.00 0.01 0.01 0.93 1.00 -0.10 -0.10 0.97 1.00 0.01 0.01 0.97 0.95
I1KMQ9 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.56 0.15 0 .97 1.00 0.00 0.00 0.93 1.00 -0.09 -0.09 0.97 1.00 0.00 0.00 0.97 0.95
K7ME55 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.64 0.07 0 .99 1.00 0.01 0.01 0.93 1.00 -0.09 -0.09 0.97 1.00 0.01 0.01 0.97 0.94
K7MPQ1 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.03 0.00 0 .98 1.00 0.00 0.00 0.93 1.00 -0.09 -0.09 0.97 0.99 0.00 0.00 0.97 0.95
I1J8V9 Unch aracterized protein
OS=Glycine max PE=4 SV=1 0.96 0.10 0 .99 1.00 0.01 0.01 0.93 1.00 -0.09 -0.09 0.97 0.99 0.01 0.01 0.97 0.94
I1K5F0 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.39 0.04 0 .98 1.00 0.01 0.01 0.93 1.00 -0.09 -0.09 0.97 0.99 0.01 0.01 0.97 0.94
I1K565 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.15 0.11 0 .99 1.00 0.00 0.00 0.93 1.00 -0.09 -0.09 0.97 0.99 0.00 0.00 0.97 0.95
I1KQE8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.01 0.00 0 .98 1.00 -0.01 -0.01 0.92 1.00 -0.09 -0.09 0.97 0.99 -0.01 -0.01 0.96 0.95
K7M3E3 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.40 0.05 0 .98 1.00 0.01 0.01 0.93 1.00 -0.09 -0.09 0.97 0.99 0.01 0.01 0.97 0.94
K7KCS1 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.05 0.00 0 .98 1.00 0.59 0.73 1.00 0.96 -0.09 -0.08 0.97 0.99 0.01 0.01 0.97 0.94
I1KWC6 Uncharacterized protein
OS=Glycine max PE=4 SV=1 2.64 0.25 0 .87 1.00 0.01 0.01 0.93 1.00 -0.08 -0.08 0.97 0.99 0.01 0.01 0.97 0.94
I1LVQ5 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.01 0.00 0 .98 1.00 0.00 0.00 0.93 1.00 -0.08 -0.08 0.97 0.99 0.00 0.00 0.97 0.95
I1K1C9 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.98 -0.10 1.00 0.98 0.0 1 0.01 0.93 1.00 -0.08 -0.08 0.97 0.99 0.01 0.01 0.97 0.94
C6SV85 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.70 0.20 0 .94 1.00 0.01 0.01 0.93 1.00 -0.08 -0.08 0.97 0.99 0.01 0.01 0.97 0.94
K7M2P3 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.52 0.15 0 .97 1.00 0.00 0.00 0.93 1.00 -0.08 -0.08 0.97 0.99 0.00 0.00 0.97 0.95
I1LRQ4 Unch aracterized protein
OS=Glycine max PE=4 SV=1 0.41 0.04 0 .98 1.00 0.02 0.02 0.94 1.00 -0.08 -0.08 0.97 0.99 0.02 0.02 0.98 0.94
K7MZR8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.44 0.14 0 .98 1.00 0.00 0.00 0.93 1.00 -0.07 -0.07 0.97 0.99 0.00 0.00 0.97 0.95
K7L2I5 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.20 0.02 0 .98 1.00 0.37 0.45 1.00 0.96 -0.07 -0.07 0.97 0.99 0.03 0.03 0.99 0.94
I1JGR4 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.12 0.12 0 .99 1.00 0.61 0.77 1.00 0.96 -0.07 -0.07 0.97 0.99 0.36 0.45 1.00 0.91

S38
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
I1J7G5 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -2.60 -0.25 1.00 0.84 - 0.90 -1.41 1.00 0.80 -0.04 -0.07 0.98 0.99 -0.45 -0.82 0.92 0.99
K7MKC3 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.42 0.04 0 .98 1.00 -0.13 -0.26 0.89 1.00 -0.03 -0.06 0.98 0.99 -0.13 -0.26 0.88 0.99
I1L5K0 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.23 -0.03 0.98 1.00 0.3 6 0.45 1.00 0.96 -0.06 -0.06 0.98 0.99 0.02 0.03 0.98 0.94
I1N696 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.42 -0.15 1.00 0.89 0.4 4 0.80 1.00 0.96 -0.04 -0.06 0.98 0.99 -0.20 -0.30 0.88 0.99
K7LJS8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.09 0.11 0 .99 1.00 0.03 0.03 0.94 1.00 -0.06 -0.05 0.98 0.99 0.03 0.03 0.98 0.94
K7L730 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -1.85 -0.20 1.00 0.83 - 0.05 -0.16 0.89 1.00 -0.02 -0.05 0.98 0.99 -0.15 -0.48 0.88 1.00
K7MUF4 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.97 0.22 0 .91 1.00 -0.01 -0.01 0.92 1.00 -0.03 -0.05 0.98 0.99 -0.01 -0.01 0.96 0.95
I1LMP2 Unch aracterized protein
OS=Glycine max PE=4 SV=1 1.71 0.18 0 .96 1.00 -0.11 -0.23 0.89 1.00 -0.01 -0.02 0.99 0.99 0.01 0.02 0.98 0.94
I1LBD0 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.75 0.17 0 .96 1.00 -0.09 -0.20 0.89 1.00 0.04 0.07 1.00 0.97 -0.09 -0.20 0.89 0.98
I1MBR7 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.88 0.09 0 .99 1.00 -0.05 -0.25 0.89 1.00 0.05 0.24 1.00 0.97 -0.24 -1.29 0.99 0.87
C6T0M2 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.24 0.02 0 .98 1.00 -0.29 -0.74 0.94 1.00 0.11 0.29 1.00 0.97 -0.21 -0.56 0.88 1.00
I1JSF3 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.71 -0.08 0.99 1.00 0.0 0 -0.01 0.93 1.00 0.20 0.31 1.00 0.97 0. 00 -0.01 0.96 0.95
K7LKJ0 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.81 0.08 0 .99 1.00 -0.16 -0.29 0.89 1.00 0.25 0.43 1.00 0.97 -0.16 -0.29 0.88 0.99
I1N484 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.47 -0.04 0.98 1.00 0.5 7 0.73 1.00 0.96 0.40 0.45 1.00 0.97 0.32 0.40 1.00 0.91
K7LJB3 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.36 -0.04 0.98 1.00 0.3 6 0.43 1.00 0.96 0.42 0.46 1.00 0.97 0.02 0.02 0.98 0.94
K7K5A8 Uncharacterized protein
OS=Glycine max PE=4 SV=1 2.81 0.28 0 .83 1.00 0.02 0.02 0.93 1.00 0.42 0.47 1.00 0.97 0.02 0.02 0.98 0.94
I1NF29 Uncharacterized protein
OS=Glycine max PE=4 SV=1 -0.10 -0.01 0.98 1.00 0.3 4 0.42 1.00 0.96 0.43 0.49 1.00 0.97 0.34 0.42 1.00 0.91
C6T0L2 Uncharacterized protein
OS=Glycine max PE=4 SV=1 2.17 0.20 0 .94 1.00 0.31 0.87 1.00 0.97 0.24 0.60 1.00 0.97 -0.08 -0.20 0.89 0.98
K7LYD7 Uncharacterized protein
OS=Glycine max PE=4 SV=1 1.98 0.22 0 .91 1.00 0.00 0.01 0.93 1.00 0.17 0.61 1.00 0.98 0.04 0.14 1.00 0.93
I1K5E5 Uncharacterized protein
OS=Glycine max PE=4 SV=1 0.82 0.09 0 .99 1.00 -0.25 -0.34 0.89 1.00 0.41 0.62 1.00 0.98 0.20 0.31 1.00 0.91
I1LZ82 Unch aracterized protein
OS=Glycine max PE=4 SV=1 1.69 0.17 0 .96 1.00 -0.05 -0.30 0.89 1.00 0.19 1.11 1.00 0.99 -0.26 -1.52 1.00 0.79
I1KCD7 Uncharacterized protein
OS=Glycine max PE=4 SV=2 1.75 0.19 0 .94 1.00 -0.54 -2.05 1.00 0.48 -0.89 -2.55 1.00 0.32 -0.85 -2.87 1.00 0.26
I1K6C9 Uncharacterized protein
OS=Glycine max PE=4 SV=2 1.75 0.18 0 .96 1.00 -0.67 -2.03 1.00 0.49 -0.85 -2.07 1.00 0.54 -0.67 -2.03 1.00 0.57
I1JXQ5 Uncharacterized protein
OS=Glycine max PE=4 SV=2 -1.19 -0.12 1.00 0.96 - 0.37 -0.60 0.92 1.00 -1.04 -1.17 1.00 0.94 -0.37 -0.60 0.89 1.00
I1K4F3 Uncharacterized protein
OS=Glycine max PE=4 SV=2 -1.78 -0.18 1.00 0.85 - 0.59 -0.76 0.95 1.00 -0.68 -0.74 0.95 1.00 -0.59 -0.76 0.91 1.00

S39
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
I1L362 Uncharacterized protein
OS=Glycine max PE=4 SV=2 1.98 0.19 0 .95 1.00 0.02 0.03 0.94 1.00 -0.37 -0.49 0.93 1.00 -0.77 -1.01 0.95 0.95
I1JAY8 Uncharacterized protein
OS=Glycine max PE=4 SV=2 -1.95 -0.19 1.00 0.83 - 0.33 -0.41 0.89 1.00 -0.43 -0.46 0.93 1.00 -0.33 -0.41 0.88 1.00
I1LK14 Uncharacterized protein
OS=Glycine max PE=4 SV=2 -1.00 -0.10 1.00 0.99 0.4 6 0.66 1.00 0.96 -0.42 -0.44 0.93 1.00 -0.34 -0.41 0.87 1.00
I1J6K4 Unch aracterized protein
OS=Glycine max PE=4 SV=2 0.38 0.04 0 .98 1.00 0.00 0.00 0.93 1.00 -0.41 -0.42 0.93 1.00 -0.33 -0.40 0.87 1.00
I1JY87 Unch aracterized protein
OS=Glycine max PE=4 SV=2 -1.53 -0.15 1.00 0.88 - 0.24 -0.48 0.90 1.00 -0.15 -0.27 0.93 1.00 -0.75 -1.30 0.99 0.87
I1JF86 Uncharacterized protein
OS=Glycine max PE=4 SV=2 1.23 0.12 0 .99 1.00 0.00 -0.01 0.93 1.00 -0.07 -0.18 0.95 1.00 - 0.06 -0.17 0.90 0.97
I1K5S7 Uncharacterized protein
OS=Glycine max PE=4 SV=2 -0.16 -0.02 0.98 1.00 0.9 3 1.28 1.00 0.98 -0.12 -0.12 0.96 1.00 1.31 1.89 1.00 0.95
I1JTT1 Unch aracterized protein
OS=Glycine max PE=4 SV=2 -0.38 -0.03 0.98 1.00 - 0.01 -0.01 0.92 1.00 -0.11 -0.10 0.97 1.00 -0.01 -0.01 0.96 0.95
I1KN71 Uncharacterized protein
OS=Glycine max PE=4 SV=2 1.32 0.13 0 .98 1.00 -0.01 -0.01 0.93 1.00 -0.10 -0.10 0.97 1.00 -0.01 -0.01 0.96 0.95
I1JCE8 Uncharacterized protein
OS=Glycine max PE=4 SV=2 -0.40 -0.04 0.98 1.00 0.0 1 0.02 0.93 1.00 -0.10 -0.09 0.97 1.00 0.01 0.02 0.98 0.94
I1MQT7 Uncharacterized protein
OS=Glycine max PE=4 SV=2 -2.10 -0.23 1.00 0.83 0.0 0 0.00 0.93 1.00 -0.09 -0.08 0.97 0.99 0.00 0.00 0.96 0.95
I1J8K1 Unch aracterized protein
OS=Glycine max PE=4 SV=2 0.11 0.01 0 .98 1.00 0.01 0.01 0.93 1.00 -0.02 -0.04 0.99 0.99 -0.26 -0.50 0.88 1.00
I1LJ01 Uncharacterized protein
OS=Glycine max PE=4 SV=2 -0.17 -0.02 0.98 1.00 0.1 2 0.43 1.00 0.96 0.11 0.36 1.00 0.97 0.00 0.01 0.97 0.94
I1LXQ1 Uncharacterized protein
OS=Glycine max PE=4 SV=2 -0.33 -0.03 0.98 1.00 0.1 0 0.23 1.00 0.97 0.30 0.63 1.00 0.98 0.10 0.23 1.00 0.92
T2DNM1
Uncharacterized protein
OS=Phaseolus vulgaris PE=2
SV=1
1.81 0.19 0.95 1.00 0.00 0.00 0.93 1.00 -0.40 -0.42 0.93 1.00 -0.32 -0.39 0.87 1.00
T2DLE1
Uncharacterized protein
OS=Phaseolus vulgaris PE=2
SV=1
-2.42 -0.23 1.00 0.83 -0.03 -0. 03 0.92 1.00 0.72 0.87 1.00 0.98 -0.03 -0.03 0.95 0.95
L0LIZ4
Uncharacterized protein
OS=Rhizobium tropici CIAT 899
GN=RTCIAT899_CH10025 PE=4
SV=1
0.02 0.00 0.98 1.00 -1.33 -1.84 1.00 0.59 -1.42 -1 .62 1.00 0.76 -1.33 -1.84 1.00 0.65
T2DMU1
Vacuolar-sorting receptor
OS=Phaseolus vulgaris PE=2
SV=1
3.31 0.35 0.89 1.00 -0.59 -1.38 1.00 0.81 -0.46 -0 .98 0.98 1.00 -1.03 -2.04 1.00 0.57
D7EYG6 V-H(+)-ATPase subunit A
OS=Glycine max PE=2 SV=1 2.46 0.25 0 .87 1.00 0.00 0.01 0.93 1.00 -0.10 -0.26 0.93 1.00 -0.05 -0.15 0.91 0.97
A7L5M4
Voltage-dependent anion channel
(Fragment) OS=Phaseolus vulgaris
PE=2 SV=1
0.04 0.00 0.98 1.00 -1.15 -2.50 1.00 0.26 -1.69 -2 .55 1.00 0.32 -1.50 -2.88 1.00 0.26
I1MSU1 Xylose isomerase OS=Glycine
max PE=3 SV=1 0.51 0.06 0.98 1.00 0.64 1.10 1.00 0.97 0.62 0.97 1.00 0.98 0.21 0.32 1.00 0.91
T2DP67 Xylose isomerase OS=Phaseolus
vulgaris PE=2 SV=1 -1.56 -0.14 1.00 0.90 0.22 0.48 1.00 0.95 -0.02 -0.03 0.99 0.99 0.02 0.03 0.99 0.94
L0LRX3
XylR-type repressor
OS=Rhizobium tropici CIAT 899
GN=RTCIAT899_PB00875 PE=4
2.72 0.25 0.86 1.00 0.01 0.01 0.93 1.00 -0.07 -0.07 0.98 0.99 0.01 0.01 0.97 0.94

S40
Protein ID Description
62.5 mg/kg
n
ano
-
CeO
2
125 mg/kg
n
ano
-
CeO
2
250
mg/kg
n
ano
-
CeO
2
500 mg/kg
n
ano
-
CeO
2
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
Log fold
change
a
Zstatistic
b
FDR
up
c
FDR
down
d
SV=1
T2DM51
Zinc finger CCCH domain-
containing protein OS=Phaseolus
vulgaris PE=2 SV=1
-0.10 -0.01 0.98 1.00 -0.10 -0. 30 0.89 1.00 0.30 0.95 1.00 0.98 -0.35 -1.02 0.95 0.95
a LogFold: Log fold change of treatment vs control
b Z-statistic: The significance statistic for differential expression
c FDR: Local false discovery rate
d FDRrup: False discovery rate for the over-expression in treatment, proteins with an FDR ≤ 0.1 are highlighted in green
e FDRdown: False discovery rate for the under-expression in treatment, proteins with an FDR ≤ 0.1 are highlighted in red

S41
Table S7. List of proteins exclusively identified in nano-CeO
2
exposures at all concentration
and their gene ontology.
Protein ID Description
Gene Ontology
Biological process Molecular
function
Cellular
component
C6SW24 Uncharacterized
protein
Proteolysis involved in Cellular protein
catabolic process; threo nine-type
endopeptidase activity
Threonine-type
endopeptidase
activity
Proteasome
core
complex
C6SZN4 Ribosomal
protein L37 Translation
Metal ion binding;
rRNA binding;
structural
constituent of
ribosome
Ribosome
I1JGR4 Uncharacterized
protein
RNA binding
I1NF29 Uncharacterized
protein Serine-type endo/exopeptidase activity
Serine-type
endo/exopeptidase
activity
L0LS48 Chemotaxis
protein CheW Chemotaxis; signal transducer activity Signal transducer
activity Intracellular
O24323 Cysteine
proteinase Cysteine-type peptidase activity Cysteine-type
peptidase activity

S42
Table S8. Gene ontology of the proteins regulated by nano-CeO
2
(62.5- 500 mg/kg)
exposure to kidney bean plants.
Protein
ID
Protein Name
Gene ontology
nano-CeO2 (mg/kg soil)
Cellular
component
Biological
process
Molecular
function 62.5 125 250 500
UP-REGULATION
O24319 Purple acid
phosphatase
dephosphorylation,
metabolic
processes
acid phosphatase
activity, hydrolase
activity, metal ion
binding
F8QXP9 Defensin defense response
DOWN-REGULATION
Q43632 Phaseolin nutrient reservoir
activity
P84869 Antifungal lectin PVAP defense response
I1MC31 Uncharacterized
protein
protein folding ATP binding
-
Q9M7M4 Mannose lectin FRIL carboh ydrate
binding
-
P07219 Phaseolin, alpha-type nutrient reservoir
activity
- -
Q41115 Alpha-phaseolin nutrient reservoir
activity
- -
P24459 ATP synthase subunit
alpha
Mitochondria ATP synthesis
coupled proton
transport
-
-
P02853 Phaseolin, beta-type nutrient reservoir
activity
- -
I1KPN3 Uncharacterized
protein
ATP binding
- -
F8QXP7 Legumin nutrient reservoir
activity
- -
C6TGA8 Putative
uncharacterized
protein
carbohydrate
metabolic process
hydrolase activity,
hydrolyzing O-
glycosyl
compounds
- -
Q41119 Peptidyl-prolyl cis-
trans isomerase
protein folding peptidyl-prolyl cis-
trans isomerase
activity
- -
P05087 Leucoagglutinating
phytohemagglutinin
defense response mannose binding
- -
Q43628 Phytohemagglutinin carbohydrate
binding
- -
C6TAB7 Uncharacterized
protein
intramolecular
transferase activity;
cellulose
biosynthetic
process
-4.87 - - -

S43
Protein
ID
Protein Name
Gene ontology
nano-CeO2 (mg/kg soil)
Cellular
component
Biological
process
Molecular
function 62.5 125 250 500
I1L0N6 Uncharacteri zed
protein
- -
-
Q9FQF9 Lipoxygenase fatty acid
biosynthetic
process, oxidation
reduction
iron ion binding,
oxidoreductase
activity
- - -
T2DNN1 Steroid binding protein heme binding
- - -
B3TDK6 Lipoxygenase fatty acid
biosynthetic
process, oxidation
reduction
iron ion binding,
oxidoreductase
activity
- - -
A7L3U9 Elongation factor 1-
alpha
translation GTP binding
- - -
Q01899 Heat shock protein protein folding stress response ATP binding
- - -
Green boxes indicate up-regulation and the red boxes indicate down-regulation at FDR ≤ 0.1.

S44
References
1. Majumdar, S.; Peralta-Videa, J. R.; Bandyopadhyay, S.; Castillo-Michel, H.; Hernandez-
Viezcas, J.-A.; Sahi, S.; Gardea-Torresdey, J. L., Exposure of cerium oxide nanoparticles to
kidney bean shows disturbance in the plant defense mechanisms. J. Hazard. Mater. 2014,
278, 279-287.
2. Packer, A. P.; Lariviere D Fau - Li, C.; Li C Fau - Chen, M.; Chen M Fau - Fawcett, A.;
Fawcett A Fau - Nielsen, K.; Nielsen K Fau - Mattson, K.; Mattson K Fau - Chatt, A.; Chatt
A Fau - Scriver, C.; Scriver C Fau - Erhardt, L. S.; Erhardt, L. S. Validation of an inductively
coupled plasma mass spectrometry (ICP-MS) method for the determination of cerium,
strontium, and titanium in ceramic materials used in radiological dispersal devices (RDDs).
Anal. Chim. Acta 2007, 588,166-172.
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