ArticlePDF Available

Concentration induced properties of silver nanoparticles and their antibacterial study

Authors:
Emmanuel Ugwoke at University of Nigeria
Emmanuel Ugwoke
Samson O. Aisida at University of Nigeria
Samson O. Aisida
Dr. Ameer Ahmed Mirbahar at Shah Abdul Latif University, Khairpur
Dr. Ameer Ahmed Mirbahar
  • Shah Abdul Latif University, Khairpur
mohamed arshad
mohamed arshad
mohamed arshad
  • This person is not on ResearchGate, or hasn't claimed this research yet.
Show all 7 authorsHide
Download full-text PDFDownload full-text PDF
Read full-text
Download citation

Abstract

Green synthesis of silver nanoparticles (Ag-NPs) using Moringa Oleifera (MO) bloated in flavonoids, tannins, and phenols phytochemical as a capping and reducing agent which reduced the silver ions to Ag-NPs without any external reducing agent for an antibacterial agent is enclave in this work. The effects of the reducing agent in the different concentrations of silver nitrate incubated for 72 h on the structural, morphological and optical properties followed by the antibacterial activity of Ag-NPs were studied. The synthesized Ag-NPs were characterized by scanning electron microscope (SEM), transition electron microscope (TEM), X-ray dispersive spectroscopy (XRD), Uv–visible spectroscopy and Fourier transform infrared spectroscopy (FT-IR) analysis. The TEM analysis gave a spherical morphology with particle size ranged between 4 and 12 nm. The XRD gave a face-centered cubic phase with the crystalline structure of Ag-NPs. The FTIR shows the functional groups responsible for the reducing agent. The UV–visible confirm the formation of Ag-NPs with surface plasmon resonance (SPR) absorbance peak 450 nm. To our knowledge, the biocidal activities of AgNPs have been studied in details but the biosynthesized formulation of Ag-NPs via MO against Coliform bacterial strain compared with Gentamicin (a strong antibacterial drug) has not been reported in the literature. It is noteworthy that the biocidal activities of Ag-NPs show effective and functional susceptibility to the Ag-NPs against the bacterial strains at lower concentrations compared with Gentamicin. Hence, the obtained Ag-NPs via facile and innocuous synthesis could serve as an auspicious and efficient antibacterial agent against human pathogenic strains due to its non-toxicity, biocompatible, eco-friendly, cost-effectiveness, and facile protocols in therapeutic biomedical fields.
Concentration induced properties of silver nanoparticles and their antibacterial study _ Elsevier Enhanced Read
er.pdf
Content uploaded by Samson O. Aisida
Author content
All content in this area was uploaded by Samson O. Aisida on Dec 20, 2019
Content may be subject to copyright.
Concentration induced properties of silver nanoparticles and their antibacterial study _ Elsevier Enhanced Read
er.pdf
Content uploaded by Samson O. Aisida
Author content
All content in this area was uploaded by Samson O. Aisida on Dec 13, 2019
Content may be subject to copyright.
... In the UV-Vis spectrum the maximum peak intensity observed at 466 nm confirms the green synthesis of silver nanoparticles. These finding was is in agreement with data in the UV-Visible spectrum of [24,25]. The broadness of the peak indicates the presence of polydispersed nanoparticles particles as discussed by Ibrahim et al. in (2021) [26] who attributed the broadness of absorption band due to an increasing of a particle size distribution. ...
... Also, Kota et al.in (2017) [13] reported that the presence of reactive N-H and O-H groups that are effective in reducing Ag(I) ions to Ag (0) So, presence of flavones, terpenoids, polysaccharides, proteins and aromatic groups of polyphenols indicating the role of reduction potential of these compounds in bio-reduction, stability and capping of AgNPs, Green synthesized silver nanoparticles showed good antibacterial activities against S. aureus and P. aeruginosa. Also, Prasad and Elumalai in (2011) and Ugwoke et al. in (2020) reported the excellent antibacterial activity of green synthesized AgNPs using Moringa oleifera leaf extract [12,24]. So, ecofriendly green synthesized silver nanoparticles have an effective antibacterial activity against different bacterial strains. ...
... Also, Kota et al.in (2017) [13] reported that the presence of reactive N-H and O-H groups that are effective in reducing Ag(I) ions to Ag (0) So, presence of flavones, terpenoids, polysaccharides, proteins and aromatic groups of polyphenols indicating the role of reduction potential of these compounds in bio-reduction, stability and capping of AgNPs, Green synthesized silver nanoparticles showed good antibacterial activities against S. aureus and P. aeruginosa. Also, Prasad and Elumalai in (2011) and Ugwoke et al. in (2020) reported the excellent antibacterial activity of green synthesized AgNPs using Moringa oleifera leaf extract [12,24]. So, ecofriendly green synthesized silver nanoparticles have an effective antibacterial activity against different bacterial strains. ...
Antibacterial Potential of Bacterial Cellulose Impregnated with Green Synthesized Silver Nanoparticle Against S. aureus and P. aeruginosa
Article
Full-text available
  • Jan 2023
  • CURR MICROBIOL
In this study, bacterial cellulose (BC) impregnated with green synthesized silver nanoparticles (AgNPs) is evaluated as an antimicrobial membrane for wound-healing treatment. Green synthesized silver nanoparticles using Moringa oleifera leaf extract were characterized using UV‒visible spectroscopy, FTIR, X-ray diffraction, and transmission electron microscopy. The results confirmed that the resulted particles were Ag 2 O and metallic Ag in nanoscale with an average size ranged from 24 to 40 nm. The green synthesized nanoparticles incorporated within both bacterial cellulose and filter paper discs showed excellent antibacterial activities against Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 9027. There was no significant difference noticed between bacterial cellulose and filter paper holding capacity to nanoparticles and there was lack of interaction between bacterial cellulose and impregnated nanoparticles as elaborated by Fourier transform infrared spectral analyses. Scanning electron microscopy investigation showed major distortions effects of green synthesized silver nanoparticles on bacterial cell morphology.
View
... Nanoparticle biosynthesis is an ecological, green, and non-toxic method of processing that involves microorganisms. It is particularly used in the synthesis of iron oxide, silver, nickel oxide, copper oxide nanomaterials, etc. [19][20][21]. The significant progress in recent years is linked on the one hand to the development and refinement of nanoparticle synthesis methods, and on the other hand, to the development of more complex characterization techniques and specific and efficient analysis methodologies. ...
Advances of Nanoparticles and Thin Films
Article
Full-text available
  • Aug 2022
Nanoparticles and thin films are currently among the most active research fields in materials sciences for technological applications [...]
View
View
Innovative utilization of harvested mushroom substrate for green synthesis of silver nanoparticles: A Multi–Response optimization approach
Article
  • Jan 2024
  • ENVIRON RES
optimization study for the green synthesis of silver nanoparticles (AgNPs). A multiple response central composite design with three parameters: pH of the reaction mixture, temperature, and incubation period at three distinct levels was employed in the optimization study. The particle size of AgNPs, UV absorbance, and the percentage of Ag/Cl elemental ratio were considered as the response parameters. For each response variable examined the model used was found to be significant (P < 0.05). The ideal conditions were: pH 8.9, a temperature of 59.4 °C, and an incubation period of 48.5 h. The UV–visible spectra of AgNPs indicated that the absorption maxima for AgNP–3 were 414 nm, 420 for AgNPs–2, and 457 for AgNPs-1. The XRD analysis of AgNPs-3 and AgNPs-2 show a large diffraction peak at ∼38.2°, ∼44.2°, ∼64.4°, and ∼77.4°, respectively, which relate to the planes of polycrystalline face-centered cubic (fcc) silver. Additionally, the XRD result of AgNPs–1, reveals diffraction characteristics of AgCl planes (111, 200, 220, 311, 222, and 400). The TEM investigations indicated that the smallest particles were synthesized at pH 9 with average diameters of 35±6 nm (AgNPs–3). The zeta potentials of the AgNPs are −36 (AgNPs–3), −28 (AgNPs–2), and −19 (AgNPs–1) mV, respectively. The distinct IR peak at 3400, 1634, and 1383 cm−1 indicated the typical vibration of phenols, proteins, and alkaloids, respectively. The AgNPs were further evaluated against gram (+) strain Bacillus subtilis (MTCC 736) and gram (−) strain Escherichia coli (MTCC 68). All of the NPs tested positive for antibacterial activity against both bacterial strains. The study makes a sustainable alternative to disposing of HMS to achieve the Sustainable Development Goals (SDGs).
View
View
View
Comparison of antibacterial activity and cytotoxicity of silver nanoparticles and silver-loaded montmorillonite and saponite Author links open overlay panel
Article
  • May 2023
  • APPL CLAY SCI
Although silver nanoparticles are known for their antibacterial activity, little research has been carried out on what synthesis method provides the most effective particles. In this study, silver nanoparticles were synthesised via chemical reduction by using silver nitrate as the silver precursor, ascorbic acid as the reducing agent and sodium citrate as the stabilising agent. The solutions were adjusted to several pH values employing sodium hydroxide, citric acid or nitric acid. Dynamic light scattering and absorption spectra in the ultraviolet/visible region characterisation revealed that employing nitric acid to adjust the pH produced more varied and larger silver particle sizes. Then, silver nanoparticles were supported on montmorillonite and saponite through wet impregnation or ion exchange methods. Scanning electron microscopy, energy-dispersive X-ray spectroscopy and transmission electron microscopy characterisation confirmed that silver nanoparticles were successfully loaded onto the clay minerals. Next, the antibacterial activity of the samples was evaluated against Escherichia coli and Staphylococcus aureus by determining their minimum inhibitory concentrations and minimum bactericidal concentrations. The free silver nanoparticles did not show any antibacterial activity at 125 mg/L. In contrast, the silver-loaded samples obtained by wet impregnation and with a higher silver content displayed the strongest antibacterial effect. Finally, the cytotoxicity of the samples was determined in GM07492-A cell line by using an XTT colorimetric assay. The calculated IC50 values revealed that the supported silver nanoparticles were barely toxic. Thus, the silver-loaded clay minerals obtained here are promising antibacterial materials with a high-grade safety profile.
View
View
Cesium lead bromide (CsPbBr3) perovskite nanocrystals for sensing applications
Chapter
  • Aug 2022
Metal halide perovskite nanomaterials have attracted much attention in recent years for diverse applications. Especially, excellent materials properties associated with the metal halide perovskites such as large absorption coefficient, higher photoluminescent quantum yield, long carrier lifetime, long diffusion coefficient have stimulated huge interest among the researchers for the optoelectronic applications. With the general formula AMX3 (where M = RNH3⁺, Cs⁺; M = Pb2 +, Sn2 +, Ge2 +, etc. X = Cl, Br, I), multi-direction research on the all-inorganic perovskite nanomaterials is intensively focused by several research groups. There are wide range of possibilities in the detection of several toxic gases, explosives for the military purpose by these materials. With high sensitivity and selectivity of analytes, perovskites are showing outstanding results in fabricating sensors in several promising fields. Because of the high stability and excellent optical properties, cesium lead bromide perovskite NCs (CsPbBr3 NCs) are highly preferred and intensively researched for sensing purpose. In this scenario, this chapter is discussing about the current developments and future perspectives of cesium lead bromide perovskite nanocrystals for sensor applications.
View
Green synthesized silver nanoparticles for the sensing of pathogens
Chapter
  • Jan 2022
The indiscriminate and continuous use of chemical substances to control pathogens negatively impacts the environment and human health. In this sense, silver nanoparticles are promising materials to be used as sensors for pathogen detection or applied as a biocide to inhibit microbial growth in plant crops. The main characteristics of silver nanoparticles for pathogen detection are high surface area, sensitivity, specificity, and precision. Therefore, these properties provide the versatility of the detection of nanobiosensor-based methods such as colorimetric, chemosensor, fluorescent, electrochemical, and optical. The synthesis of nanoparticles from biological sources is a green alternative to traditional chemical means of synthesis. Among the various green sources explored for the production of nanoparticles, microalgae have gained prominence in recent years. Microalgae are photosynthetic microorganisms that synthesize silver nanoparticles in their culture by reducing silver through enzymes, biochemical compounds with reducing or antioxidant properties. Thus, this chapter presents the green and simultaneous production of silver nanoparticles and biomass from microalgae technology. Besides, it explored the antimicrobial properties of microalgal biomass and silver nanoparticles produced by microalgae and their ability to act as a biocide. The chapter also addresses the use of silver nanoparticles for pathogen sensors and the challenges regarding the nanoparticles synthesized by microalgae as an ecological and sustainable processes.
View
Incubation period induced biogenic synthesis of PEG enhanced Moringa oleifera silver nanocapsules and its antibacterial activity
Article
Full-text available
  • Aug 2019
Human pathogenic diseases are on the rage in the list of enfeebling diseases globally. The endless quest to salvage these drug-resistant pathogens ravaging our system through various therapies still posts serious challenge. This study engaged a biogenic synthesis that is benign, facile, biocompatible, cost-effective and eco-friendly to synthesized silver nanocapsule (AgNCs) via Moringa oleifera aqueous extract under incubation control. The flavonoid-kaempferol, phenolic-chlorogenic acid and tannin components of MO acted as the potential stabilizing and reducing agent in the formation of AgNCs. The formulated AgNCs was further functionalized with PVA, PVP and PEG for biocompatibility and dispersion enhancement. Various characterization techniques were used to determine the properties of AgNCs formulated. The absorbance due to the color change was observed by the UV-Visible spectroscopy with surface plasmons resonance peak between 425 and 455 nm. The Fourier transform infrared spectroscopy (FTIR) shows the various functional group responsible for the biogenic synthesis of AgNCs. The X-ray spectroscopy analysis shows a single phase cubic structure of AgNCs formed. The Scanning electron microscopy (SEM) image shows a rod-like nanocapsule of uniform grains. The antibacterial potency of AgNCs was proven against gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Coliform). The AgNCs inhibited the growth of the three human pathogens with Coliform showing the highest activity to the AgNCs with a minimum inhibitory dose of 15 μg/mL. It is noteworthy that the bacterial strains show functional susceptibility to the AgNCs at lower concentrations compared to the conventional antibacterial drugs. Consequently, AgNCs serve as an enhanced substitute for the conventional antibacterial drugs in therapeutic biomedical field sequel to its pharmacodynamics against the bacterial strains.
View
Biosynthesis of iron oxide nanoparticles via a composite of Psidium guavaja-Moringa oleifera and their antibacterial and photocatalytic study
Article
Full-text available
  • Aug 2019
Human pathogenic diseases are on the rampage in the list of debilitating diseases globally. The endless quest to salvage this menace through various therapies via innocuous agents is essential to overcome these drug-resistant pathogens. This study engaged a benign, facile, biocompatible, cost-effective and eco-friendly approach to synthesized iron oxide nanoparticles (FeO-NPs) via a composite of Psidium guavaja-Moringa oleifera (PMC) leaf extract to address six most debilitating bacterial strain in vitro as an antibacterial agent. Physicochemical analysis of PMC formed nanoparticles (PMC_NPs) was effectuated through Fourier Transform Infrared Spectroscopy (FT-IR), UV-Visible Spectroscopy, X-ray Diffraction Spectroscopy (XRD), Transmission Electron Microscopy (TEM), and Vibrating Sample Magnetometer (VSM). The PMC_NPs inhibited the growth of six human pathogens with higher activity at lower concentrations. It is noteworthy from our observations that, the bacterial strains show functional susceptibility to the PMC_NPs at lower concentrations compared to the orthodox antibacterial drugs. Photocatalytic degradation was observed with a decrease in the absorbance of Methylene blue dyes with the help of PMC_NPs apropos irradiation time under visible light irradiation. Consequently, PMC_NPs serve as an enhanced substitute for the orthodox antibacterial drugs in therapeutic biomedical field sequel to its pharmacodynamics against the bacterial strains at lower concentrations and also serves as a good component for water purification.
View
Biogenic synthesis and antibacterial activity of controlled silver nanoparticles using an extract of Gongronema Latifolium
Article
Full-text available
  • Jul 2019
  • MATER CHEM PHYS
Biogenic synthesis of silver nanoparticles (AgNPs) was conducted via an aqueous extract of fresh leaf of Gongronema Latifolium (FLGL) an herbaceous flowering plant as a fuel agent. The effects of different AgNO3 concentration with the fixed volume of FLGL on the properties of the formulated AgNPs were investigated. Also, the antibacterial activity AgNPs formed was observed through the solid agar plates supplemented with different concentrations of nano-sized AgNPs on Gram-negative (Coliform, Escherichia coli), and Gram-positive (Staphylococcus Aurea) bacterial strain. We observed from the various characterizations of AgNPs that the variation in the concentration of AgNO3 coupled with the incubation period played a major role in controlling the particle sizes and dispersions. The UV–visible confirmed the formation of AgNPs with the surface Plasmon resonance (SPR) peak value at 430 nm in the visible region showing the type as well as the nature of the particles in the colloidal solution. The Fourier transform infrared spectroscopy revealed the functional groups liable for the biogenic process of AgNPs. The X-ray diffraction pattern shows a single-phase crystalline structures in size range of 9–31 nm for the three concentrations. The Scanning and transmission electron microscopies results indicate the spherical shape of the formed AgNPs with proper coating from the bioactive compounds and a diameter of 19 nm for the highest concentration. The energy-dispersive X-ray spectroscopy analysis shows a strong signal for silver. It is noteworthy that the colloidal solutions obtained show effective therapeutic ability of the AgNPs against the bacterial strains using FLGL as a reducing agent for the first time. A bactericidal was observed through the inhibitory zone, against the bacterial strains, with a higher activity above Ciprofloxacin (the positive control). Hence, the biocompatible, nontoxicity and eco-friendly formulated AgNPs is auspicious and efficient as a therapeutic tool for antibacterial activities against human pathogens.
View