Saida Boudaoud

Université Paris-Sud 11 | Paris 11

Objective Primary Sjögren's syndrome (pSS) is characterised by chronic hyperactivation of B lymphocytes. Salivary gland epithelial cells (SGECs) could play a role in promoting B-lymphocyte activation within the target tissue. We aimed to study the interactions between SGECs from patients with pSS or controls and B lymphocytes. Methods Patients had...

Background Primary Sjögren’s syndrome (pSS) is an auto-immune disorder characterized by lymphocytic infiltrates and destruction of the salivary glands. Mechanisms leading to B lymphocytes chronic activation remain partially understood and we assumed that salivary gland epithelial cells (SGECs) might play a key role in B lymphocytes activation and d...

Career situation of first and presenting author Student for a master or a PhD. Introduction Primary Sjögren’s syndrome (pSS) is a chronic auto-immune disorder characterized by lymphocytic infiltrates and destruction of the salivary glands (SG). Chronic B cell activation, the secretion of autoantibodies and the critical role of BAFF have been demon...

Background Primary Sjögren’s syndrome (pSS) is a chronic auto-immune disorder characterised by lymphocytic infiltrates and destruction of the salivary glands (SG). Chronic B cell activation, the secretion of autoantibodies and the critical role of BAFF have been demonstrated. However, mechanisms leading to B cells dysregulation remain partially und...

Background Primary Sjögren’s syndrome (pSS) is a chronic autoimmune disorder characterised by lymphocytic infiltrates and destruction of the salivary glands. Several lines of evidence support the hypothesis that salivary gland epithelial cells (SGECs) are not only the target of autoimmunity in pSS patients but may also play a role for its initiatio...

Introduction Patients with rheumatoid arthritis (RA) are exposed to an increased risk of lymphoma and the impact of treatments is difficult to assess. Tofacitinib, an oral Janus Kinase (Jak) 3 and 1 inhibitor that has shown positive results in RA patients, may impair NK-cell function due to its inhibitory action on IL2 and IL15 signalling. Objecti...

Introduction Risk of lymhoma in patients with rheumatoid arthritis (RA) and disease activity is the main risk factor. The impact of treatment, notably of anti-TNF, is unclear: decreasing the risk of lymphoma by controlling activity or alterring anti-tumour immunosurveillance. Anti-TNF are not associated with an increased risk of lymphoma in large e...

Objective MicroRNAs (miRNAs) play an important role in the pathogenesis of autoimmune diseases such as primary Sjögren’s syndrome (pSS). This study is the first to investigate miRNA expression patterns in purified T and B lymphocytes from patients with pSS using a high-throughput quantitative PCR (qPCR) approach. Methods Two independent cohorts of...

Background: An interferon (IFN) signature is involved in the pathogenesis of primary Sjögren's syndrome (pSS), but whether the signature is type 1 or 2 remains controversial. Mouse models and genetic studies suggested the involvement of T helper 1 and type 2 IFN pathways. Likewise, polymorphisms of interleukin 12A gene (IL-12A), which encodes for...

Background An interferon (IFN) signature is involved in the pathogenesis of primary Sjögren's syndrome (pSS), but whether the signature is type 1 or 2 remains controversial. Mouse models and genetic studies suggested the involvement of T helper 1 and type 2 IFN pathways. Likewise, polymorphisms of interleukin 12A gene (IL-12A), which encodes for IL...

Background Risk of lymhoma in patients with rheumatoid arthritis (RA) and disease activity is the main risk factor. The impact of treatment, notably of anti-TNF, is unclear: decreasing the risk of lymphoma by controlling activity or alterring anti-tumor immunosurveillance. Anti-TNF are not associated with an increased risk of lymphoma in large epid...

Objectives: Rheumatoid arthritis (RA) is associated with an increased risk of lymphoma linked to activity of the disease. Immunosuppressive drugs have been suspected to induce an additional risk. Since, NK cells have been recently shown to participate to anti-lymphoma immunosurveillance, we aimed to assess if anti-TNF might impact their anti-lymph...

Objective The relevance of the Th17 pathway in primary SS (pSS) is unclear. Published studies have relied on restimulating circulating CD161⁺ T cells in vitro for quantitation of IL-17–producing cells. While CD161 marks all IL-17⁺ T cells, it is also expressed by other Th subsets. The aim of this study was to directly analyse retinoic acid receptor...

Background Rheumatoid arthritis (RA) is associated with an increased risk of lymphoma linked to activity of the disease. Immunosuppressive drugs have been suspected to induce an additional risk. Since, NK cells have been recently shown to participate to anti–lymphoma immunosurveillance, we aimed to assess if anti-TNF might impact their anti-lymphom...

Background Primary Sjögren Syndrome (pSS) is an autoimmune disease mediated by B-cells as evidenced by the occurrence of non-Hodgkin lymphoma mainly involving salivary glands and increased B-cell activation markers in the serum of pSS patients. A type I interferon response is also observed among some patients. However, some mice models and large ge...

Background: Mass cytometry has recently emerged as a promising tool for clinical research. However, few studies have demonstrated its benefit for patient stratification and biomarker identification. Primary Sjögren's syndrome (pSS) is a prototype of chronic autoimmune disease, the pathogenesis of which remains unclear and for which treatment does...

Rheumatoid arthritis (RA) is the most common chronic inflammatory rheumatic condition over the world. RA is potentially disabling because chronic inflammation of the joints leads to joint destruction. To date, the best predictor of radiographic progression for patients with early RA is the presence of radiographic erosions at baseline, but a limite...

The recent interest for interleukin-33 (IL-33) in primary Sjogren syndrome (pSS) pathophysiology is due to three studies,1–3 which have shown an increase in IL-33 serum levels in patients with pSS compared with controls. IL-33 is a member of the IL-1 family which is released in case of cellular damage and acts as an alarmin; however, its role in pS...

A germline and coding polymorphism (rs2230926) of TNFAIP3 (A20), a central gatekeeper of nuclear factor-kappa B (NF-kB) activation, was recently found associated with primary Sjögren's syndrome (pSS)-associated lymphoma in a French cohort. We aimed to replicate this association. The rs2230926 polymorphism was genotyped in cases and controls of Euro...

Objectives: To investigate DKK-1 and SOST serum levels among patients with recent inflammatory back pain (IBP) fulfilling ASAS criteria for SpA and associated factors. Methods: The DESIR cohort is a prospective, multicenter French cohort of 708 patients with early IBP (duration >3 months and <3 years) suggestive of AxSpA. DKK-1 and SOST serum le...

Objectives: Non-Hodgkin lymphoma (NHL) is a severe complication of primary Sjogren's syndrome (pSS). Salivary glands ectopic germinal centers (GC) are predictors of NHL occurrence. Given the association between CCL11 and CXCL13 and ectopic GC, we assessed the link between these chemokines and NHL in pSS patients and the association between these c...

Beyond genetics, epigenetics alterations and especially those related to DNA methylation, play key roles in the pathogenesis of autoimmune diseases such as primary Sjögren's syndrome (pSS) and systemic lupus erythematosus. This study aimed to assess the role of methylation deregulation in pSS pathogeny through a genome-wide methylation approach. 26...

Background The cellular characterization of the immune infiltrate in exocrine glands from patients with primary Sjögren's syndrome (pSS) has relied so far on methods with low dimensionality. As a result, our knowledge of cellular events taking place in the exocrine glands of these patients is limited. Objectives In order to quantify cellular varia...

There is growing interest in the role of Dickkopf-1 (DKK-1), an inhibitor of the Wnt signalling pathway, in subchondral bone erosions in rheumatoid arthritis (RA). de Rooy et al1 have previously reported that polymorphisms within the DKK-1 locus might contribute to RA structural severity. In fact, rs1896368 was significantly associated with an incr...

Background: HCV chronic infection is associated with an increased risk of non-Hodgkin lymphoma (NHL) occurrence. HCV-associated NHL share homologies with primary Sjögren Syndrome (pSS)-associated NHL, and especially an association with chronic antigenic stimulation and with auto-antibody presence, especially rheumatoid factor (RF) and mixed cryoglo...

Background The pathophysiology of lymphomas in auto immune disease (AID) involves persistent inflammation and activation of autoimmune B cells leading to NF-kB activation. The TNFAIP3 gene encodes the A20 protein, a central gatekeeper of NF-kB activation. Germinal abnormalities in TNFAIP3 have been associated with different AID and somatic mutation...

Several autoimmune diseases, including primary Sjögren's syndrome (pSS), are associated with an increased risk for lymphoma. Polymorphisms of TNFAIP3, which encodes the A20 protein that plays a key role in controlling nuclear factor κB activation, have been associated with several autoimmune diseases. Somatic mutations of TNFAIP3 have been observed...

Background Autoimmune result from the interaction between genetic and environmental factors. Previous studies have demonstrated the implication of DNA methylation alterations in many autoimmune diseases.1 Sjögren’s Syndrome (SS) is a prototypic systemic autoimmune disease that can be primary or associated with other systemic connective tissue disea...