Safaa Barghash

Desert Research Center | DRC · Animal Health Department

The present study aimed to determine the prevalence and distribution of flea-infested ruminants in Egypt's Northern West Coast (NWC) and South Sinai Governorate (SSG), besides the molecular characterization and genotypes of Nematode and Trematode in the identified flea species. In total, 765 animals of both sexes and ages (471 from NWC and 294 from...

Trypanosoma evansi is the most ubiquitous protozoan parasite with a wide geographical distribution. The current study aimed to investigate if Trypanosoma evansi induces immunosuppression that may interfere with the development of immunity after vaccination in rats against Pasteurella multocida. T. evansi-infected and non-infected Wester Albino rats...

Sarcocystis is a zoonotic worldwide cyst-forming coccidian parasite. The study aimed to determine the frequency of Sarcocystis infection in ruminants in South Sinai, identify Sarcocystis spp. and genotypes that can infect these animals using molecular techniques. A total of 353 blood samples were collected from ruminants to molecular screen for sar...

Background: Babesia and Theileria are the etiological agents of babesiosis and theileriosis. Among them, some species are highly pathogenic and cause serious economic losses to livestock holders, especially in desert areas of Egypt. Objective: The present study aimed to prevalent and phylogeny characterizes three subspecies, Babesia bovis, Babesia...

This study may be the first to detect the genotype of Giardia among ruminants in the South Sinai Governorate. Fresh iodine-faecal stained smears were examined by microscopy, followed by a nested PCR on frozen faecal specimens. The results showed Giardia in 40% (78/195) & 28.72% (56/195) of the examined ruminants by microscopic and nPCR, respectivel...

Toxoplasma gondii is a coccidian parasite known for its heavy toll on people and livestock. It can cause abortion and a variety of congenital diseases. The current study aimed to examine some seroprevalence and molecular attributes of T. gondii obtained from ruminants in the North-West of Egypt. Specimens were random selected from five different lo...

Objective: The present study aimed to evaluate the antitrypanosomal activity of Rosmarinus officinalis L. and Salvia fruticosa essential oils (EOs) against Trypanosoma evansi. Methods: The chemical compositions of these oils were elucidated by GC and GC/MS analysis and assayed for their in vitro and in vivo antitrypanosomal activity. Different conc...

Neither physiological nor pathological changes following treatments explained why trypanosomes in the same group of experimentally treated animals correlated in virulence. Also, they behaved like each other but not similar to other groups despite the same T. evansi injected strain. The current study aims to discuss whether molecular changes might o...

Echinococcus granulosus, hosted by humans and animals, impose serious human health risk and cause significant livestock production loss. It is widely seen endemically in the Middle East countries.The objective of this study was to investigate the disease epidemics in humans and livestock animals (camels, cattle, buffaloes, sheep, goat, pigs and don...

Echinococcus granulosus, hosted by humans and animals, impose serious human health risk and cause significant livestock production loss. It is widely seen endemically in the Middle East countries.The objective of this study was to investigate the disease epidemics in humans and livestock animals (camels, cattle, buffaloes, sheep, goat, pigs and don...

It is the first one had been conducted in Egypt to evaluate the antitrypanosomal activity of human plasma proteins compared with diminazene aceturate (DA) using Albino rats as a biological model. It was also carried out to assess the behavior, hematological, biochemical, and histopathological changes that may be associated with the administration o...

Follow-up surveys of the blood parasites of the livestock and their possible vectors are recommended to assess their distribution and infection rates in different areas in Egypt and to plan control measures against their vectors. By this study, the authors have completed a comprehensive survey of the extent of Trypanosoma evansi infection in North-...

This study aimed to assess the antitrypanosomal activity of leaf ethanolic extracts against Trypanosoma evansi experimental infection. Seven medicinal plants: Camellia sinensis, Thymus vulgaris, Mentha longifolia, Azadirachta indica, Olea europoea, Rosmarinus officinalis and Saliva officinalis, were selected based on information from traditional he...

An epidemiological study was conducted on the occurrence of blood parasites infection in local camels in three selected sites representing northern west coastal zone of Egypt, using Giemsa-stain blood smears (GSBS) and Polymerase chain reaction (PCR). This is the first molecular diagnosis report to give a picture of blood parasites covering these a...

An epidemiological study was conducted on the occurrence of blood parasitic infection in local camels in three selected sites representing Northern West Coastal zone of Egypt using Giemsa-stain blood smears (GSBS) and Polymerase chain reaction (PCR). This is the first molecular diagnosis report, which gives a picture of blood parasites covering thi...

Tick-borne pathogens become healthy important as the incidence of tick-borne diseases increases and the geographic areas in which they are found expand. A relatively little information is available on ticks infesting camels in Matrouh Governorate, Egypt, and the role of ticks as disease vectors. Thus rendering PCR assay the only viable alternative...

Trypanosoma evansi, the agent of trypanosomiasis commonly known as Surra or Guffar, is regarded as one of the most economically important animal parasitic pathogen affecting livestock in Egypt. The current study aims to discuss genetic characterization and phylogenetic analysis of Trypanosoma isolates from local and imported naturally infected came...

In Egypt, two local strains of dromedaries' camels (Sudanese and Maghrabi) are different from each other in some morphological, physiological properties and their origin. Trypanosoma evansi was well studied in imported and local Sudanese camels. No published studies exist on trypanosomosis in Maghrabi camels originating from North Africa, despite s...

A polymerase chain reaction (PCR-based) assay was evaluated for detection of Trypanosoma evansi DNA in experimentally infected mice and naturally infected camels, sheep and goats using the set of primers TBr1 & TBr2 that amplified 164 bp DNA fragment. The results revealed that PCR- based assay was able to detect T. evansi directly from the blood du...

The present study was conducted to evaluate the use of polymerase chain reaction (PCR) using (Rode Trypanozoon antigen type) RoTat 1.2 primers for the genomic DNA of -eight Egyptian strains of T. evansi from camels, goats, sheep, cattle and donkeys from different areas of Egypt. In addition, six previously identified isolates of T. evansi, from Afr...

The present study was conducted to evaluate the use of polymerase chain reaction (PCR) using (Rode Trypanozoon antigen type) RoTat 1.2 primers for the genomic DNA of-eight Egyptian strains of T. evansi from camels, goats, sheep, cattle and donkeys from different areas of Egypt. In addition, six previously identified isolates of T. evansi, from Afri...