S Verma

Indian Council of Medical Research | ICMR · National Institute of Pathology (NIP)

Breast cancer is the most frequently diagnosed disease causing most deaths in women worldwide. Chemotherapy and neo-adjuvant therapy are the standard method of treatment in early stages of breast cancer. However drug resistance in breast cancer limit the use of these methods for treatment. Research focus is now shifted towards identifying natural p...

Book series on Medical Science gives the opportunity to students and doctors from all over the world to publish their research work in a set of Preclinical sciences, Internal medicine, Surgery and Public Health. This book series aim to inspire innovation and promote academic quality through outstanding publications of scientists and doctors. It als...

HER2 is the membrane receptor tyrosine kinase showing overexpression in several human malignancies, particularly breast cancer. HER2 overexpression causes the activation of Ras- MAPK and PI3K/Akt/ NF-κB cellular signal transduction pathways that lead to cancer development and progression. HER2 is, therefore, presumed as one of the key targets for t...

Introduction: Microtubules are intracellular, filamentous, polymeric structures that extend throughout the cytoplasm, composed of α-tubulin and β-tubulin subunits. They regulate many cellular functions including cell polarity, cell shape, mitosis, intracellular transport, cell signaling, gene expression, cell integrity, and are associated with tum...

Aromatase enzyme plays a fundamental role in the development of estrogen receptors, and due to this functionality, the enzyme has gained significant attention as a therapeutic for reproductive disorders and cancer diseases. The currently employed aromatase inhibitors have severe side effects whereas our novel aromatase inhibitor is more selective a...

Background Cyclooxygenase enzyme is frequently overexpressed in various types of cancer and found to play a crucial role in poor prognosis in cancer patients. In current research, we have reported the new COX-2 inhibitors for cancer treatment using computer-aided drug design and experimental validation.MethodsA total of 12,795 compounds from the di...

Aromatase enzyme plays a fundamental role in the development of estrogen receptors and due to this functionality, the enzyme has gained significant attention as a therapeutic for reproductive disorders and cancer diseases. The aromatase inhibitors, currently in clinical use, have such serious side effects that it is crucial to find novel aromatase...

Cyclooxygenase‐2 (COX‐2) is a key aspect of the physiology and pathogenesis of various cancer types. Overexpression of this enzyme is responsible for the elevated prostaglandin production and characteristic feature of breast cancer. Inhibition of COX‐2 derived prostanoids facilitates anti‐inflammatory, analgesic, and antipyretic effects of non‐ster...

Purpose: Proteoglycans (PGs) are negatively charged macromolecules containing a core protein and single or several glycosaminoglycan chains attached by covalent bond. They are distributed in all tissues, including extracellular matrix (ECM), cell surface, and basement membrane. They are involved in major pathways and cell signalling cascades which...

Personalized medicine involves the practice to deliver customized service to the recipients based on some specific factors related to the patients. Knowledge of proper genetic information, lifestyle, and environment may help to select the proper therapy, dose, or regimen. Precision medicine has the potential to modify the treatment procedure as per...

Nature-inspired computing (NIC) is a relatively new concept to design new algorithms for solving complex problems based on natural phenomenon. It is a stochastic search technique that is successfully applied in diverse applications in the medical domain and predominantly provides small to large-scale problem-solving solutions. NIC is an emerging ap...

Abstract Background and Purpose: The aromatase enzyme plays a fundamental role in addressing the development of estrogen receptors and giving attention to the therapy of reproductive disorders and cancer diseases. In clinical use, the objectionable effects found in these target inhibitors are indispensable in finding novel aromatase inhibitors with...

Punicalagin is the most bioactive pomegranate polyphenol with high antioxidant and free-radical scavenging activity and can potentially cure different ailments related to the cardiovascular system. The current research work was envisioned to predict the targeting efficiency of punicalagin (PG) nanoparticles to the macrophages, more specifically to...

Punicalagin is the most bioactive polyphenols of pomegranate with high antioxidant, FRS activity. It has the potential to cure different ailments related to the CVS system. The current research work was envisioned to predict the targeting efficiency of punicalagin (PG) nanoparticles to the macrophages, more specifically to bone marrow macrophages....

Breast cancer is a heterogeneous disease and the most frequent malignancy in women globally each year. Emerging applications of gene expression profiles are important to determine the clinical outcomes for breast cancer. Biomarker dataset from Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), and Oncomine databases are used to analyze...

Background Breast cancer ranks first in women and the second most common type of cancer overall. It is the most important barrier to the rise of life expectancy, globally affecting disease modalities. Cyclooxygenase-2 (COX-2) has become a prominent hallmark as inhibition target for breast cancer, and this therapeutic target for anti-inflammatory dr...

Computational intelligence (CI) approaches are multi-layered neural networks having robust reasoning, logical, and self-regulating capability to learn that highly simulate the human cognitive process. CI technologies such as machine learning (ML), deep learning (DL), and artificial intelligence (AI) algorithms appeared to be the promising solution...

Cancer is a highly morbid disease affecting 20% of the world population. The third most common type of cancer is oral cancer which develops in the tissues of the mouth and neck and has different survival rates in men and women. Oral squamous cell carcinomas (OSCC), are the most common type of oral cancer and its early detection is key to longevity,...

Computational Intelligence (CI) methods are being widely used in different fields of studies including medical sciences and oncology research. CI methods are applied in different facets of oncology including tumor classification, early prediction and diagnosis, survival prediction, modeling and simulation of cell growth, therapy optimization, and o...

Human EGFR (Epidermal Growth Factor Receptor) family of tyrosine kinase receptors consists of four members, ErbB1-4. Abnormalities in the ErbB family characterize a variety of human cancers, including breast cancer. Tyrosine kinase is recruited by the activated EGFR cell surface receptor, which transmits signals from the receptor to interact with i...

Shedding of infectious coronavirus disease (COVID-19) is affecting 215 countries and territories; quickly circulates continuously in worldwide. The vital scientific communities are rigorously looking at these public health challenges, global crisis and finding new ways to deal with this pandemic disease. Currently, there is no specific effective ap...

In pancytopenia, the number of cells reaches to white blood cell count (WBC) < 3500 cells/mm³, hemoglobin (Hb) < 11 g/dL and platelet count <150000 cells/mm³. Nanomedicine is the specific area of medicine that comprises of tools and knowledge of nanotechnology in the prevention, diagnosis, control and treatment of life-threatening disease. It paved...

Objective In this research work, we hypothesized to predict the nanoparticulate system, best suited for targeted delivery of filgrastim. Significance: Targeted delivery of filgrastim to bone marrow is required to decrease the incidence of neutropenia/febrile neutropenia. This is achieved by nanoparticulate systems, duly designed by bioinformatics a...

Health problems are increasing worldwide pertaining to cancer modalities. Cyclooxygenase enzyme is known to be involved in cancer biology, neurological disorders, cardiovascular and other diseases. It has been a promising target for developing novel anti-inflammatory drugs in breast cancer treatment. Hence, a computer-aided drug design strategy was...

Background: The regulatory T cell (Treg) is essential for prevention of autoimmunity. In a preliminary study, we showed significant deficiency of Tregs (CD4CD25 T cells) in rheumatic heart disease (RHD) patients (an autoimmune disease), but the markers used could not reliably differentiate Treg from nonregulatory conventional T cells (Tcon). The s...

Nasopharyngeal carcinoma (NPC) is an epithelial tumour with a distinctive racial and geographical distribution. High incidence of NPC has been reported from China, Southeast Asia, and northeast (NE) region of India. The immune mechanism plays incredibly role in pathogenesis of NPC. Tumour necrosis factors (TNFs) and heat shock protein 70 (HSP 70) c...

High incidence of nasopharyngeal carcinoma (NPC) has been reported from China, Southeast Asia and Northeast (NE) region of India. Populations at geographic regions having higher incidence of NPC display human leukocyte antigen (HLA) distribution patterns different from areas having low incidence. The current study has investigated the contribution...

Autoimmunity plays an essential role in the pathogenesis of rheumatic heart disease (RHD). The CD4+CD25+ T cell (Tregs) is the chief regulator of autoimmunity, and is essential for the induction and maintenance of self-tolerance and prevention of autoimmunity. To date, the levels of Tregs in RHD have not been investigated. Hence, the study aim was...

Tulsi (Ocimum sanctum Linn.) is considered as a sacred herb and traditionally it is believed that consumption of Tulsi leaf on empty stomach increases immunity. Experimental studies have shown that alcoholic extract of Tulsi modulates immunity. The present study was designed to evaluate the immunomodulatory effects of ethanolic extract of Tulsi lea...

Non muscle invasive bladder cancers recur frequently and identification of biomarkers for predicting recurrence are necessary. The present study evaluated the individual and synergistic effects of tumor suppressor (p53/p21waf1) and angiogenesis [vascular endothelial growth factor (VEGF)/endoglin (CD105)] markers. The study included 90 cases of non...

Combination therapy with intravesical bacillus Calmette-Guerin (BCG) plus interferon-alpha2b (IFN-alpha2b) for superficial transitional cell carcinoma (TCC) seems to be immune-dependent and activation of Th1 immune response is required for clinical efficacy. The present study evaluates circulating serum cytokine profiles (Th1/Th2 cytokines IFN-gamm...

Acute myeloid leukemia (AML) with normal karyotype represents a clinically and molecularly heterogeneous disease. Molecular markers with prognostic significance have been examined to improve risk profile characterization of this group. Activating mutations on FLT3 receptor are one of the most common genetic alterations reported. However, the preval...

Occurrence of aberrant phenotypes in childhood and adult acute leukemia (AL) differs considerably in independent studies and their association with prognostic factors is still controversial. In the present study, 214 patients with AL (106 children and 108 adults) were evaluated for the aberrant expression of CD33 in ALL (B cell and T cell) and CD3,...

Global ischemia was induced in gerbil by bilateral occlusion of the common carotid arteries for 5 min. Sodium ionophore monensin or sodium channel blocker tetrodotoxin (TTX) was administered at doses of 10 micorg/kg, i.p., 30 min before ischemia induction; the dose was repeated after 22 hr. Subsequently, brain infarct occurred, determined at 24 hr...

The antioxidant activity of C.oil in cerebral stroke has been reported earlier. We have attempted here to clarify the mechanisms underlying the neuroprotection against experimental cerebral ischemia by Curcuma oil (C.oil), isolated from the rhizomes of Curcuma longa. C.oil (250 mg/kg i.p.) was given 30 min before focal ischemia in rats caused by oc...

Turmeric is a source of numerous aromatic compounds isolated from powdered rhizomes of Curcuma longa Linn. The constituents are present as volatile oil, the Curcuma oil (C.oil), semi-solid oleoresins and non-volatile compounds such as curcumin. A rapidly expanding body of data provides evidence of the anti-cancer action of Curcumin, and most import...

Evaluation of in vitro spontaneous apoptosis of acute leukemic blast cells after incubating for different time period and its correlation with clinical outcome is well documented in the literature. However, there is insufficient information available on the flowcytometric determination of cell viability immediately after separating blast cells and...

Malignant nodular hidradenomas are rare variants of sweat gland carcinomas characterized by aggressive clinical behaviour. We report a case of 42-year-old female with 3 x 2 x 1 cm nodule on her foot followed by cutaneous and regional lymph node metastasis. Flow cytometry showed S-phase fraction of 72.6% and 15.8% of aneuploid cells corroborating wi...

Recent report from our laboratory showed that A549 cells representing alveolar epithelial cells produce chemokine interleukin-8 and nitric oxide (NO) when challenged with Mycobacterium tuberculosis. Interferon-gamma (IFN-gamma) played a critical role in priming these cells to generate NO in vitro. In the present study, we report that M. tuberculosi...

Transitional cell carcinoma (TCC) is the commonest cancer of the bladder. Although majority of TCC can be diagnosed at an early stage and removed easily by transurethral resection of tumor (TURT), the management of this carcinoma is complicated due to frequent recurrences usually within 6 months to one-year period. An imbalance between the Th1 and...

Human genital infection caused by Chlamydia trachomatis is thought to be immunologically mediated, resulting in local recruitment of lymphocyte subsets and inducing the production of cytokines. Little information is available about the role of lymphocyte recruitment and the regulation of cytokine production in the genital tract of C. trachomatis po...

To investigate changes in cell-mediated immunophenotypes by flow cytometry in endocervical secretions and peripheral blood in women with Chlamydia trachomatis infection. Fifty women attending the gynaecology outpatient department of Safdarjang Hospital, New Delhi, India, with signs and symptoms of cervicitis were enrolled. All patients underwent en...

FlowCytometric DNA analysis and cytological diagnosis of pleural effusions. Veena kashayap and S.Verma

Objectives: To analyse the functional significance of Nitric oxide (NO) in cardiovascular regulation from rostral ventral medulla in cats using nitric oxide precursor, donor and guanylate cyclase inhibitor, methylene blue. Methods: Arterial pressure was recorded from femoral artery of chloralose anaesthetized cats through statham P23 DC transducer...