Rong-Rong Lu

Jiangnan University · School of Food Science and Technology

Anaplastic thyroid cancer (ATC) is the most aggressive thyroid cancer. Current approaches including surgery, chemotherapy and therapeutic drugs provide limited benefit for ATC patients. Diallyl trisulfide (DATS) has been documented as a promising anti-cancer agent in various carcinomas. However, its role in ATC tumorigenesis remained unclear. Our r...

The objective of our research was to evaluate the molecular mechanism of the anti-inflammatory effects of surface layer protein (Slp) derived from Lactobacillus acidophilus NCFM in lipopolysaccharide-induced RAW264.7 cells. Our results presented that Slp, with an apparent size of 46 kDa, attenuated the production of TNF-α, IL-1β, and reactive oxyge...

This study aimed to elucidate the potential effects of diallyl trisulfide (DATS) on human papillary thyroid carcinoma BCPAP cells and its underling mechanisms. DATS is one of the organosulfur compounds which derived from garlic. In this study, we demonstrated that compared to the solvent control, DATS treatment at concentrations of 5, 10 and 20 μΜ...

Capsaicin (CAP), a potent transient receptor potential vanilloid type 1 (TRPV1) agonist, is a major ingredient of red pepper. Recently, capsaicin has attracted more and more attention owing to its multiple biological activities. However, the anticancer effects of capsaicin against various types of cancers, especially on thyroid carcinoma, have not...

Casein-dextran nanoparticles (CDNs) were prepared from casein-dextran conjugates by heating in a dry/wet state and then adjusting the pH to the isoelectric point of the protein (pH 4.6) to investigate their physicochemical characteristics. The effects of the dry and wet heating methods on the properties of the CDNs were also evaluated. The CDNs wer...

The objective of this study was to explore the antioxidant effect of the surface layer proteins (SLPs) and their mechanism. We investigated four SLPs which were extracted from L. casei zhang, L. rhamnosus, L. gasseri and L. acidophilus NCFM respectively using LiCl. The protective effect of SLPs on H2O2-induced HT-29 cells oxidative injury was inves...

Intestinal pathogens have been proposed to adhere to epithelial cells and cause apoptosis. This study was to investigate the inhibitory effects of surface layer protein (SLP, 46kDa) from Lactobacillus acidophilus NCFM on Escherichia coli and Salmonella-induced apoptosis in HT-29 cells and the mechanism of the inhibition was also studied. The SLP co...

Curcumin, a major active component of Curcuma longa, is a natural polyphenolic antioxidant compound which has a strong potential for cancer prevention and treatment. However, the effect of curcumin on papillary thyroid carcinoma cells has not been investigated. In the present study, we report that curcumin induces cell death of papillary thyroid ca...

The aim of this study was to investigate the murein hydrolase activities of the surface layer proteins (SLPs) from two strains of Lactobacillus acidophilus using zymography. The influence of these hydrolase activities on Escherichia coli ATCC 43893 was also evaluated by analysing their growth curve, cell morphology and physiological state. After th...

Background Maillard reaction is widely used to improve the functional properties or biological activities of food. The purpose of this study was to investigate the effect of Maillard reaction on angiotensin I-converting enzyme (ACE) inhibitory activity in a casein hydrolysate-xylose system.ResultsTwo-step hydrolysis was used to prepare casein ACE i...

High‐pressure processing at mild temperatures ( HPMTs ) could be an alternative to thermal processing for destroying pressure‐resistant bacteria and achieving commercial sterilization. In this study, carrot juice was pressure‐treated at 300, 400 and 500 MPa for 15 min at 40, 50 and 60 C , and afterward stored at 4, 25 and 37 C . The results showed...

Curcumin, traditionally used as food and medicinal purposes, has recently been reported to have protective efficacy against hypoxia. Hypoxia is one of the important reactive factors in tumor metastasis, which is a key problem in clinical thyroid cancer therapy. In present study, we investigate the anti-metastatic effect of curcumin on the K1 papill...

The relationship between hydrophobicity and the protective effect of whey protein hydrolysates (WPHs) against oxidative stress was studied. Whey protein was first hydrolysed by pepsin and trypsin to obtain WPHs. After absorbed by macroporous adsorption resin DA201-C, three fractions named as M20, M40, and M60 were eluted by various concentrations o...

The objective of this study was the characterisation of the surface layer proteins (SLPs) and their functional role in the probiotic activity of L. helveticus fb213, L. acidophilus fb116 and L. acidophilus fb214. SLPs were extracted and identified by SDS-PAGE, circular dichroism spectra and LC-MS analysis. The results revealed that the molecular mas...

Whey protein hydrolysates (WPHs) were prepared with pepsin and trypsin. A PC12 cell model was built to observe the protective effect of WPHs against H2O2-induced oxidative stress. The results indicated that WPHs reduced apoptosis by 14% and increased antioxidant enzyme activities. Flow cytometry was used to assess the accumulation of reactive oxyge...

An exopolysaccharide (EPS-3) was isolated from the culture of Lactobacillus planterum LP6 and purified by ion exchange and gel chromatography. The concentrations required to scavenge 50% of the initial radical for DPPH, OH and O2(-) radicals were 1.38, 3.43 and 0.11mg/mL, respectively. The reducing power (A700nm) was 0.632 at 5mg/mL. The cell viabi...

Curcumin, the active constituent of dietary spice turmeric, possesses a strong potential for cancer prevention and treatment. However, there is no study to address the effects of curcumin on invasion and metastasis of thyroid cancers. Thyroid cancer is the most common malignancy of endocrine organs, and its incidence rates have steadily increased o...

To isolate and identify antioxidant peptides from enzymatically hydrolysed whey protein, whey protein isolate was hydrolysed by different protease (trypsin, pepsin, alcalase 2·4L, promatex, flavourzyme, protease N). The hydrolysate generated by alcalase 2·4L had the highest antioxidant activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, su...

The anti-metastatic effect of curcumin on papillary thyroid cancer K1 cells and its underlying mechanisms were investigated. Curcumin at 12.5, 25 and 50 μM promoted mesenchymal-epithelial transition and decreased the migration rate of K1 cells by 24-87 %. Its mechanism may involve the up-regulation of E-cadherin expression levels and down-regulatio...

The protective effect of whey protein hydrolysates (WPHs) against H(2)O(2)-induced oxidative damage on rat pheochromocytoma line 12 (PC12) cells was studied. Whey protein was hydrolyzed by pepsin and trypsin and purified by macrospore absorption resins. PC12 cells were pretreated with WPHs (from 369 to 1,980 Da) at different concentrations for 2 h,...

Recently, many studies on health benefits associated with curcumin have been reported. In this study, the effects of curcumin on apoptosis of papillary thyroid cancer cell line K1 and its potential mechanisms were investigated. Curcumin was found to significantly inhibit cell viability and promoted cell apoptosis in a dose-dependent manner. Moreove...

Curcumin has been traditionally used in China and India for food and medicinal purposes. It has been shown to possess potent antioxidative activity both in vitro and in vivo. In the present study, the neuroprotective effects and the potential mechanisms of curcumin against H2O2-induced oxidative stress in mouse neuroblastoma Neuro-2A cells were inv...

Hempseeds have not been utilised to any significant extent by the industrial food markets. A novel antioxidant peptide derived from hempseed by-product was investigated. Hempseed protein isolate was prepared by alkaline extraction and subsequent isoelectric precipitation. Its enzymatic hydrolysate, obtained by Alcalase® treatment, was subjected to...

Bioactive peptides can be defined as protein fragments with potential biological activities. Milk proteins are precursors of many different biologically active peptides. Bioactive peptides from milk proteins are considered potential modulators of various regulatory processes in the body. They mediate physiological functions in cardiovascular, nervo...

  Alpha-lactalbumin (α-La), a globular protein found in all mammalian milk, has been used as an ingredient in infant formulas. The protein can be isolated from milk using chromatography/gel filtration, membrane separation, enzyme hydrolysis, and precipitation/aggregation technologies. α-La is appreciated as a source of peptides with antitumor and a...

The water-insoluble pentosan (WIP) and gluten were extracted, and the interactions of WIP and gluten at different water level and ionic strength were measured by small amplitude oscillation tests and atomic force microscopy (AFM), and visualized by scanning electron micrographs (SEM). The results showed that the viscoelasticity of gluten and gluten...

Lactoferrin (LF) was isolated from bovine colostrum by ultrafiltration and then purified with a fast flow strong cation exchange chromatography system in a production scale. A two-step ultrafiltration process was performed with membranes of nominal molecular weight cut-offs of 100 kDa for ultrafiltration (UF) step 1 (UF-1) and 10 kDa in the UF step...