Ravi S Singh

Bihar Agriculture University · Department of Plant Breeding and Genetics

Arsenic contamination in soil and water poses significant environmental and health risks. This chapter explores biotechnological approaches for arsenic remediation. It begins by discussing arsenic-induced carcinogenesis and the role of oncogenomics in understanding its molecular mechanisms with epidemiology of arsenic contamination on community hea...

Hairy root culture (HRC) is a promising biotechnological tool for the production of plant secondary metabolites under in vitro condition. This has been harnessed for the production of several molecules of medicinal and commercial importance. The hairy root phenotype appears at the site of infection of plants by Rhizobium rhizogenes which possess ch...

Frontiers in Medicinal Chemistry is a book series devoted to reviews on research topics relevant to medicinal chemistry and allied disciplines. Frontiers in Medicinal Chemistry covers developments in rational drug design, bioorganic chemistry, high-throughput screening, combinatorial chemistry, compound diversity measurements, drug absorption, drug...

Advances in biotechnology and genomics have empowered researchers to manipulate the molecular networks involved in various plant processes enabling plants to exploit resources efficiently. For photosynthetic capacity (PC), several approaches are followed to improve the PC involving conventional and molecular genetic improvement such as breeding for...

Non-coding RNAs (ncRNAs) play a crucial role in the regulation of gene expression thereby impacting several biological processes and traits. Among ncRNAs, microRNAs (miRNAs) are the most abundant class of small ncRNAs (~22 nt long transcripts), while long non-coding RNAs (LncRNAs, ≥200 bp transcripts) that do not encode proteins but have been shown...

Sabour Heera Dhan (BRR2110) is maturing in 145-150 days, semi-dwarf (~110 cm) with strong culm (lodging resistant), high tillering ability with high in panicles No./m2 (> 290 / m2), complete exerted awnless long panicles (PL=> 27.0 cm.), medium slender (MS ) grain, lodging tolerant, MR to LB, NB, Sheath blight and tolerant to LF, BPH with HRR>62.%....

Arsenic and chromium have affected wider area in the world including Gangetic plains of India due to its toxicity and carcinogenic characteristics. Entry of As(III) into food chain has also escalated problem. A novel approach has been adopted to develop remediation technique using bacteria and herbs. The bioremediation study showed Bixa orellana as...

RNA interference is an evolutionary highly conserved process of post-transcriptional gene silencing by which double stranded RNA causes sequence-specific degradation of mRNA sequences. It is anewtechnology of gene suppression whose machinery and biological functions are only moderately understood. Old-style plant breeding has been very prosperous i...

Rapid surge in the demands of medicinal plants for crude or refined drug owing to their valuable bioactive molecules has created unbearable pressure on medicinal plants flourishing in nature. Many of medicinal plants are not sufficient to meet the market demand, even not cultivable, can only be conserved in situ. Furthermore, some medicinal plants...

An experiment was conducted for the study of heterosis and combining ability in Indian mustard, (Brassica juncea L.) using 7×7 half diallel mating design along with their parents. Data were recorded for eleven quantitative characters. Analysis of variance (ANOVA) of design of experiment showed significant differences between all the treatments for...

Thirty three accessions including four checks of Indian mustard (Brasssica juncea L.) were evaluated in randomized block design having three replication for 10 quantitative characters to study the genetic diversity and correlation at Oilseed section, Bihar Agricultural University, Sabour, Bhagalpur. Multivariate based diversity analysis was determi...

Chickpea Stunt disease (CpSd) caused by bean (pea) leaf roll virus and transmitted by aphid is an emerging disease of chickpea. In early sown crop aphid population was recorded minimum (2.4) on 1st December and gradually increased upto 41.2 on 9th January than suddenly decreased (5.6) on 8th February. During mid sown condition it was recorded maxim...

Investigations were undertaken to identify the effect of temperature on the expression of a gene involved in melanin biosynthesis (Brn1) in the dark spored fungus Alternaria brassicicola causal agent of black spot of mustard and its association with the biology, virulence, and release of extracellular enzymes such as cellulase and amylase. The expr...

The efforts are being made globally regarding the development of low-cost, eco-friendly, and novel methods of remediation of arsenic from aqueous medium and soil. Biotechnological approaches of bioaccumulation and biosorption have emerged as an important tool in the ongoing research including the latest application of novel CRISPR/Cas9 technology t...

Mastitis is the one of the most prevalent and costly disease of dairy industry. Mastitis is multi etiological and complex in nature. Among bacterial mastitis, Staphylococcal mastitis is the predominant type. Pathogenic potential of Staphylococcus depends on the numbers and combination of virulence genes and also on the geographical distribution. Th...

we report A. rhizogenes-induced hairy root formation in S. bryopteris, a medicinally and commercially important plant. A. rhizogenes strain LBA1334 co-cultivated with explants (root, rhizophore, stem portion near the root, and stem with intact fronds) for 24 and 48 h after transformation for induction of hairy roots. The induction of hairy root was...

Molecular markers are useful for plant genome analysis and have now become an important tool in crop improvement. The improvement and application of molecular markers for the detection of DNA polymorphism is one of the best significant developments in the field of molecular genetics. In modern biotechnology permit to modernize significantly the tra...

The rice (Oryza sativa L.) productivity is often adversely disturbed by several abiotic and biotic stresses such as drought, submergence, fungal, bacterial, and nematode oriented biotic diseases and pest like brown plant hopper (BPH) and stem borer (SB). The major biotic stresses such as bacterial leaf blight (BLB), sheath blight (ShB), blast, brow...

The rice (Oryza sativa L.) productivity is often adversely disturbed by several abiotic and biotic stresses such as drought, submergence, fungal, bacterial, and nematode oriented biotic diseases and pest like brown plant hopper (BPH) and stem borer (SB). The major biotic stresses such as bacterial leaf blight (BLB), sheath blight (ShB), blast, brow...

Bamboo Rice though not true rice that we eat as staple food, is aromatic, short-grain white rice from bamboo seeds. It has pale green seeds having a sticky texture and bamboo leafy flavor that is enriched several health promoting photochemicals of nature such as antioxidants, vitamins and minerals. It has several health benefits and known to contro...

Nowadays viewing the present scenario of food adulteration Sequenced Characterized Amplified Regions (SCAR) markers had emerged out as authentic reliable tool for easy authenticity of food adulterants. Spices are most subjected to adulteration and even legal bodies are relying on the test conducted via DNA profiling by SCAR marker. Furthermore, res...

Zeugodacus caudatus (Fabricius) is a pest of cucurbit plants. The present study was conducted to draw the relationship among Indian Z. caudatus populations with the other defined genetic lineage of the species. A total of 18 individuals’ mtCOI gene sequences from 3 populations of India were analysed along with 34 individuals’ mtCOI gene sequences f...

To re-confirm the tolerance and susceptibility of tolerant genotypes of Brassica juncea, IC-385686, IC- 491089, IC-312545, IC-312553 and susceptible genotypes IC-399802, IC-264131, IC-426392, Laxmi were grown in Randomized Block Design (RBD) with three replications in the field conditions and the observations was recorded for days to 50% flowering,...

Hairy root culture (HRC)-based in vitro production system has become a promising biotechnological approach in recent years. The hairy root formation is the result of Agrobacterium rhizogenes-induced pathogenesis in plants, characterized by high growth rate, growth in hormone-free media besides genetic stability. These roots can imitate intact plant...

In the present study, de novo transcriptome analysis of Selaginella bryopteris in frond and root was performed to understand the regulation of flavonoid (FL) biosynthesis. High-quality data of 5.84 and 5.86 Gb was generated for frond and root, respectively, that assembled into 94,713 and 81,567 transcripts. A total of 87,471 and 73,395 unigenes wer...

The effects of elicitors [methyl jasmonate (MeJ), salicylic acid (SA) and sodium nitropruside (SNP)] on expression of three MYB transcription factor genes (SbMYB1, SbMYB2 and SbMYB3) and flavonoid content was studied in Selaginella bryopteris. Gene expression analysis showed that SbMYB2 was responsive to MeJ as its expression increased (1.6-2.36 fo...

Secondary metabolites are the bioactive compounds of plants which have no role in the development process, but are needed for defense purposes. These are the unique sources of phytoceuticals, food additives, flavors, and other industrial materials. This secondary metabolite synthesized from the primary metabolism of the plant. These bioactive compo...

The field experiment was carried out at the research farm of Horticulture Garden, Bihar Agricultural College , Sabour, Bhagalpur during 2014-15 for assessment of genetic variability among different genotypes of cape gooseberry in India. The experiment was laid out in Randomized Block Design with three replications having twelve genotypes. Analysis...

The field experiment was carried out at the research farm of Horticulture Garden, Bihar Agricultural College , Sabour, Bhagalpur during 2014-15 for assessment of genetic variability among different genotypes of cape gooseberry in India. The experiment was laid out in Randomized Block Design with three replications having twelve genotypes. Analysis...

The edible legumes are major dietary protein in vegetarian diets. However, in most of the legumes, the biologically active secondary metabolites (SMs) such as flavonoids, tannins, alkaloids, saponins, trypsin (protease) inhibitors, phytates, hemagluttinins (lectins), oxalates, cyanogenic glycosides, cardiac glycosides, coumarins, gossypol, and othe...

Secondary metabolites showed a range of biological activity such as antioxidant, antiproliferative, immunosuppressant, anti-infective, cholesterol lowering and growth promoting also termed as bioactive molecules. Higher organisms in the process of evolution have faced a diverse kind of biotic and abiotic stresses. Various genetic consequences have...

Hydrilla verticillata (L.f.) Royle, an aquatic plant, best documented example of an inducible C4 photosynthetic system, which concentrates CO2 in the chloroplasts without enzymatic compartmentation in mesophyll and bundle sheath cells. H. verticillata is a facultative C4 plant, which shifts from C3 to C4 photosynthesis under certain conditions, but...

Kharchia Local wheat variety is an Indian salt tolerant land race known for its tolerance to salinity. However, there is a lack of detailed information regarding molecular mechanism imparting tolerance to high salinity in this bread wheat. In the present study, differential root transcriptome analysis identifying salt stress responsive gene network...

It is bactocera caudata gene sequence submitted in NCBI

Genetic divergence of 12 Cape gooseberry Indian genotypes for morphological parameters was estimated using Mahanalobis D2 -analysis. The genotypes were grouped into four clusters, the cluster-II was the largest with 5 genotypes followed by cluster I and cluster IV with 3 genotypes, and cluster III with 1 genotype. Clustering pattern indicated no as...

Genetic divergence of 12 Cape gooseberry Indian genotypes for morphological parameters was estimated using Mahanalobis D2-analysis. The genotypes were grouped into four clusters, the cluster-II was the largest with 5 genotypes followed by cluster I and cluster IV with 3 genotypes, and cluster III with 1 genotype. Clustering pattern indicated no ass...

Banana is a nutritionally and economically important crop, produced and consumed particularly in the developing countries, which often faces numerous biotic and abiotic challenges that further compounded by changing adverse climatic conditions. Conventional breeding methods contributed to some extent for the banana improvement in the past, due to l...

Banana is a nutritionally and economically important crop, produced and consumed particularly in the developing countries, which often faces numerous biotic and abiotic challenges that further compounded by changing adverse climatic conditions. Conventional breeding methods contributed to some extent for the banana improvement in the past, due to l...

Soil salinity is a major abiotic stress and salt overly sensitive (SOS) pathway plays an important role in imparting tolerance to salinity by reinstating cellular ionic equilibrium. Salt overly sensitive 1 (SOS1) gene of SOS pathway has been implicated in increasing salt tolerance in plants. In this study, a 734 bp fragment of SOS1 promoter (SbUSOS...

This work developed a protocol to remove colored metabolites and other interfering substances to facilitate RNA isolation. These metabolites otherwise hinder RNA isolation and downstream applications. The developed protocol used sodium dodecyl sulphate, ethylenediamine tetra-acetic acid, and ethanol in a definite ratio that removed the said metabol...

The present invention relates to a method of cloning stable stress tolerant superoxide dismutase using universal primers

Secondary metabolites are reported to interfere with the isolation of RNA particularly with the recipes that use guanidinium-based salt. Such interference was observed in isolation of RNA with medicinal plants rheum (Rheum australe) and arnebia (Arnebia euchroma). A rapid and less cumbersome system for isolation of RNA was essential to facilitate a...

Inventor(s): BHARDWAJ PARDEEP KUMAR [IN]; KUMAR ARUN [IN]; KISHORE AMIT [IN]; GHAWANA SANJAY [IN]; RANI ARTI [IN]; SINGH KASHMIR [IN]; SINGH HARSHARAN [IN]; SINGH RAVI SHANKAR [IN]; KUMAR HITESH [IN]; SOOD PAYAL [IN]; DUTT SOM [IN]; KUMAR SANJAY [IN]; AHUJA PARAMVIR SINGH [IN] + (BHARDWAJ, Abstract not available for EP2268661 (A2) Abstract of corre...

Geranyl pyrophosphate (GPP) and p-hydroxybenzoate (PHB) are the basic precursors involved in shikonins biosynthesis. GPP is derived from mevalonate (MVA) and/or 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway(s), depending upon the metabolite and the plant system under consideration. PHB, however, is synthesized by only phenylpropanoid (PP) pathw...

Sequence alignment (ClustalW2; http://www.ebi.ac.uk/) of the deduced amino acid sequences of arnebia cDNAs. Sequence alignment (ClustalW2; http://www.ebi.ac.uk/) of the deduced amino acid sequences of arnebia cDNAs with the respective reported sequences from other plants: (a) AeACTH (GenBank:AAU95618.1Nicotiana tabacum, GenBank:ABC74567.1Picrorhiza...

Domain and protein families in the deduced amino acid sequences of arnebia cDNAs. Domain and protein families in the deduced amino acid sequences of AeACTH, AeHMGS, AeHMGR, AeMVK, AePMVK, AeMVDD, AeGDPS, AeIPPI, AePGT, AePAL, AeC4H, and Ae4-CL.

Prediction of secondary structure of deduced amino acid sequences. Prediction of secondary structure of deduced amino acid sequences of AeACTH, AeHMGS, AeHMGR, AeMVK, AePMVK, AeMVDD, AeGDPS, AeIPPI, AePGT, AePAL, AeC4H and Ae4-CL by SOPMA. Helices, sheets, turns and coils are indicated by the longest, the second longest, the second shortest and the...

Bar diagram indicating intensities (integrated density value; IDV) of the amplicons of Figure 2b at day 4 and day 8 (panel b) as measured using Alpha DigiDoc 1000 software. Bar diagram indicating intensities (integrated density value; IDV) of the amplicons of Figure 2b at day 4 and day 8 (panel b) as measured using Alpha DigiDoc 1000 software. Erro...

Primer sequences and PCR conditions used in the present work for amplifying the desired gene from arnebia. Degenerate primer sequences and PCR conditions used for amplifying the genes; AeACTH, AeHMGS, AeMVK, AePMVK, AeMVDD, AeGDPS, AeIPPI, AePAL, AeC4H, and Ae4-CL from arnebia.

Bar diagram indicating intensities (integrated density value; IDV) of the amplicons of Figure 3a and 3b as measured using Alpha DigiDoc 1000 software. Bar diagram indicating intensities (integrated density value; IDV) of the amplicons of Figure 3a and 3b as measured using Alpha DigiDoc 1000 software. Error bar shows standard deviation of three sepa...

Size of cDNAs (partial, full-length), BLAST analysis and Domain search in deduced amino acid sequences. Size of cDNAs (partial, full-length), BLAST analysis and Domain search in deduced amino acid sequences of cDNAs of AeACTH, AeHMGS, AeHMGR, AeMVK, AePMVK, AeMVDD, AeGDPS, AeIPPI, AePGT, AePAL, AeC4H, and Ae4-CL from arnebia.

Nucleotide and deduced amino acid sequence of the genes cloned from arnebia. Nucleotide and deduced amino acid sequence of the (a) AeACTH, (b) AeHMGS, (c) AeHMGR, (d) AeMVK, (e) AePMVK, (f) AeMVDD, (g) AeGDPS, (h) AeIPPI, (i) AePGT, (j) AePAL, (k) AeC4H and (l) Ae4-CL. The amino acid sequence is represented by single-letter code under each codon. S...

Primer sequences and PCR conditions used in semi-quantitative RT-PCR-based expression analysis. Primer sequences and PCR conditions used in semi-quantitative RT-PCR-based expression analysis of AeACTH, AeHMGS, AeHMGR, AeMVK, AePMVK, AeMVDD, AeGDPS, AeIPPI, AePGT, AePAL, AeC4H, and Ae4-CL in arnebia.

Primer sequences and PCR conditions used in RACE reactions. Primer sequences and PCR conditions used in RACE reactions for cloning of full-length cDNAs of AeACTH, AeHMGS, AeHMGR, AePMVK, AeMVDD, AeGDPS, AeIPPI, AePGT, AePAL, AeC4H and Ae4-CL.