Content uploaded by Pradeep kumar Patel
Author content
All content in this area was uploaded by Pradeep kumar Patel
Content may be subject to copyright.
Antioxidant defence system in Chickpea (Cicer arietinum L.): Influence by Drought Stress Implemented at Pre- and Post- Anthesis Stage. Pradeep Kumar Patel and A. Hemantaranjan 2012 American Journal of Plant Physiology Vol.7 (4):154-163.
... Chickpea is the second largest cultivated grain legume in the world, grown in about 11.5 million hectares (Varshney et al., 2013) and first in the Mediterranean basin and South Asia that frequently experiences water stress during pod set and seed filling stage (terminal drought) in India and the Mediterranean basin, leading to a substantial yield loss (Turner et al., 2001). As there is reduction in the translocation of carbohydrates during drought stress, this leads to a change in source-sink relationship (Patel and Hemantaranjan, 2012). Over past several years, there has been a great interest in research investigating the antioxidant defence system in plant leaves, as leaf is the principle plant part which acts as one of the early sensor for the drought stress. ...
... It has been demonstrated that the antioxidative response is correlated with the tolerance of individual crop cultivars to drought (Kolarovic et al., 2009). Most of the studies reported in the literature either focussed on seedlings or if in the field then on one particular stage (Patel and Hemantaranjan, 2012;Kaur et al., 2012;Devi et al., 2012;Battana and Ghanta, 2014). The present study was conducted to study the antioxidant defence response at different developmental stages i.e. vegetative stages, pre-flowering and seed filling stages of the crop to see if there is any differential development specific response of tolerant genotypes to drought. ...
Leaf is one of the early sensors for the drought stress and is important to study drought tolerance mechanism. Activities of antioxidative enzymes and status of malondialdehyde (MDA), hydrogen peroxide (H2O2), proline and total phenols were studied in leaves of drought tolerant (PDG 3 and PDG 4) and susceptible (PBG 1, GPF 2, PBG 5, L 550 and BG1053) chickpea cultivars under irrigated and rainfed conditions at different development stages. In general, with the age of plant, the activities of superoxide dismutase (SOD) and catalase (CAT) increased but the activities of glutathione reductase (GR), ascorbate peroxidase (APX) and peroxidase (POX) decreased in leaves. With some exceptions, in general, higher status of APX and POX in leaves at vegetative stage I (30 days after sowing) and II (60 days after sowing); GR at vegetative stage II and pre-flowering stage and SOD and CAT at seed filling stages in tolerant cultivars under drought stress reflected stage specific upregulation of antioxidant defence system in them. The relatively lower activities of APX and POX in old leaves during seed filling stage make them more prone to enhanced oxidative injury than the young leaves. Lower content of hydrogen peroxide and malondialdehyde in leaves of tolerant cultivars during seed filling reflects the impact of antioxidant defence system operative at that time. The higher accumulation of proline and total phenol in leaves of tolerant cultivars might be playing important role in drought stress tolerance. These results indicated the importance of upregulation of different antioxidant enzymes at variable stages of leaf development.
... These results suggest that the activities of SOD, POD and CAT, and contents of H 2 O 2 and MDA are the most important traits for plants' ability to survive under drought stress (Zhang et al., 2011). Similar increase in SOD, POD, CAT, and APX activities plant leaves under drought conditions has also been reported by Patel and Hemantaranjan (2012). ...
... Our findings showed an increase in MDA and H 2 O 2 in both well-watered and drought-stressed plants though increase was less evident in the PGPR-treated strawberry plants. Thus, the prevention of membrane damage is related to the induction of antioxidant responses which protect the plants from oxidative damage (Patel and Hemantaranjan, 2012). ...
A study was conducted during 2011 and 2012 years to evaluate the effect of 1-aminocyclopropane-1-carboxylate (ACC) deaminase-containing, N2-fixing, and P-solubilizing plant growth-promoting bacteria on the yield and quality of strawberry
(Fragaria x ananassa Duch cv. Aromas), macro- and micro-nutrient concentrations, phytohormone content and the activities of the antioxidant enzymes under four watering regimes. Water-deficit resulted in a significant decline in chlorophyll contents, fresh
weight per berry, total soluble solids and titratable acidity, while berries’ number per plant and pH value increased. The diminishing water supply caused a gradual decrease in the plant growth, accompanied by increasing activities of drought stress markers such as
total phenolics content (TPC), antioxidant capacity (TEAC), malondialdehyde content (MDA), hydrogen peroxide (H2O2),
glutathione reductase (GR), glutathione S-transferase (GST), glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and ascorbate peroxidase (APX) in the
leaves of strawberry. Inoculations with PGPR enhanced plant growth under drought stress. The bacteria also increased TPC, TEAC, anti-oxidant enzymes activity (GR, GST, CAT, POD, SOD and APX), phytohormone (GA, SA and IAA), and the contents of N, P, K, Ca, Mg, Fe, Mn, Zn and Cu but decreased MDA and H2O2 contents which may contribute in part to activation of processes involved in the alleviation of the effect of water stress.
... Chakraborty and Pradhan (2012) have observed an initial increase in the activities of SOD and CAT in four wheat varieties under drought stress and a decline in the activity observed during the period of stress, in contrast to our results. The increase of SOD (Patel and Hemantaranjan 2012) and CAT (Patel and others 2011) activity is higher in the post-anthesis than pre-anthesis period in chickpeas under drought stress conditions. By contrast, maximum POX activity occurs during the vegetative stage in chickpeas under drought stress (Oberoi and others 2014). ...
Mutation breeding is an alternative method for developing agriculturally important crops. A large set of Sagittario bread wheat seeds (Triticum aestivum L. cv.) were exposed to gamma ray irradiation (200 Gy) to obtain drought-tolerant mutant lines. To study drought tolerance, polyethylene glycol (PEG) 6000 was applied to the M2 and M3 individuals under in vitro conditions. Except in well-watered (100%) control plants, drought stress was triggered by a 50% decrease in the irrigation water applied to M3 and M4 plants in a greenhouse. Afterwards, 11 candidate drought-tolerant lines were obtained at the M4 stage and subjected to molecular analysis. The highest percentage of polymorphisms (72.4%) was detected with Retrotransposons Microsatellite Amplified Polymorphism (REMAP) markers followed by Inter-Simple Sequence Repeat (ISSR; 62.07%) and Inter-Retrotransposon Amplified Polymorphism (IRAP) markers (52.94%). A dendrogram tree and a principal coordinate analysis (PCoA) plot classified the experimental samples into three distinctive groups. Additionally, the activities of several antioxidant enzymes were evaluated in both vegetative and flowering stages, and mutant lines showing the highest biochemical performance under stress were detected in the same group through phylogenetic analysis. Gamma ray irradiation was used to improve drought-tolerant wheat lines for forward/reverse genomic studies and marker-assisted selection in crops.
... Chakraborty and Pradhan (2012) have observed an initial increase in the activities of SOD and CAT in four wheat varieties under drought stress and a decline in the activity observed during the period of stress, in contrast to our results. The increase of SOD (Patel and Hemantaranjan 2012) and CAT (Patel and others 2011) activity is higher in the post-anthesis than pre-anthesis period in chickpeas under drought stress conditions. By contrast, maximum POX activity occurs during the vegetative stage in chickpeas under drought stress (Oberoi and others 2014). ...
As result of world population's rapid increase, avaliable agricultural lands became insufficient to meet nutritional needs and it became quite important to conduct studies on increasing productivity of plants per unit area. Especially, in recent years, it's aimed to produce plants which are more resistant to abiotic stress factors and have high nutritional value, productivity.
In this study, it's aimed to induce salt stress tolerant mutants of wheat (Triticum aestivum L.) which is one the most important agricultural products in the world, by gamma radiation. For this purpose, appropriate mutation doses determined by irradiating Guadalupe wheat seeds with various gamma radiation doses and then seeds irradiated by mutation dose and control planted to experimental land for 3 years to generate M2 and M3 generations.
Mature embryos of seeds belonging to M3 generation experimental group which were harvested from experimental land. Then we have been focusing on selecting putative salt tolerant mutant under tissue culture conditions and characterizing them using ISSR markers.
... SOD provides the first line of defense against the toxic effects of elevated levels of ROS (Gill and Tuteja, 2010). SOD activity was found in pea (Moran et al., 1994) and chickpea (Patel and Hemantaranjan, 2012) under drought stress. Several studies on various species indicated that drought stress alters the level and the activities of the enzymes involved in scavenging ROS (Ünyayar et al., 2005;Masoumi et al., 2010;Selote and Khanna-Chopra, 2010;Bhardwaj and Yadav, 2012). ...
Bedding plants are important elements in landscape construction, and their cultivation is limited by
drought stress. Improving the cultivation medium of these flowers with organic materials such as biochar can be a sustainable and environmentally friendly solution to increase their tolerance to
drought stress. For this purpose, a greenhouse research was conducted to mitigate the effects of
drought on morpho
-physiological and biochemical characteristics of African marigold by application
of rice husk biochar. The factorial experiment was conducted with two factors of irrigation treatment
at three levels of 100, 50 and 25% of the field capacity (FC) and biochar at three levels of 0, 3.5 and
7%
(w/w
) in a completely randomized design with 5 replications. The results showed that drought
decrease
d the morphological factors of shoot height, shoot dry weight, number of leaves, number of
lateral branches, number of flowers, and length and diameter of flower
s, so their lowest value were
obtained in 25% FC. Furthermore, the use of biochar improved
the morphological traits mentioned
under drought conditions. The activity of peroxidase and superoxide dismutase enzymes, hydrogen
peroxide content, proline, malondialdehyde, soluble carbohydrates, chlorophyll a, chlorophyll b, total
chlorophyll and carotenoid increased under drought conditions. The use of biochar reduced these
biochemical traits under drought conditions. Furthermore, relative water content of leaf, cell
membrane stability index and soluble proteins showed a decreasing trend under drought conditions,
and the use of biochar led to the improvement of these traits. In general, 7% biochar was more
effective than 3.5% in reducing the adverse effects of drought stress. Therefore, 7% rice husk biochar
can be recommended as a suitable soil improver to increase tolerance to drought stress in bedding
plants for landscape purposes, although more investigations are needed both under field cultivation
and at other applied amounts.
This research was carried out in order to biomolecular investigate drought tension tolerance in two wheat cultivars inoculated with plant growth promoting rhizobacteria (PGPR) in the research greenhouse of Islamic Azad University, Karaj Branch in 2013-14 cropping year. The experiment design was in the split factorial arrangement based on a randomized complete blocks design with three replications. The main plot included two levels of desirable irrigation and drought tension (deficit irrigation in the pollination stage until the end of full maturity) and sub plots consisted the PGPR bacteria at four levels: 1) inoculation seed with Azotobacter chroococcum 2) inoculation seed with Azospirillum lipoferum 3) simultaneous use of both bacteria Azotobacter + Azospirillum 4) non-application of bacteria (control) and two bread wheat cultivars consisted of Sirvan and Marvdasht. Experiment traits included the activity of superoxide dismutase antioxidant enzyme (SOD), the activity of biochemical biomarker Malondialdehyde (MDA) amount of chlorophyll a, b and a + b. Results of the experiment showed that simple effect of irrigation, cultivar and bacteria treatments was significant on all the studied traits (01 / 0≥P). The activity of superoxide dismutase enzyme (SOD) in drought tension treatment was higher than full irrigation (normal). Sirwan and Marvdasht cultivars with 3.6 and 2.7 units per gram of protein, showed the higher and lower SOD enzyme activity, respectively. Sirvan cultivar compared to Marvdasht cultivar had less amount of biomarker MDA activity (27.424 milligram on gram fresh weight of tissue) and the higher content of chlorophyll a, b and a+b with 3.255, 1.578 and 4.811 (milligram on gram fresh weight of tissue), respectively.
ResearchGate has not been able to resolve any references for this publication.